CN209673460U - A kind of automatic exhibition piece machine of Portable paraffin slice - Google Patents
A kind of automatic exhibition piece machine of Portable paraffin slice Download PDFInfo
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- CN209673460U CN209673460U CN201822020676.3U CN201822020676U CN209673460U CN 209673460 U CN209673460 U CN 209673460U CN 201822020676 U CN201822020676 U CN 201822020676U CN 209673460 U CN209673460 U CN 209673460U
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- exhibition piece
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Abstract
A kind of automatic exhibition piece machine of Portable paraffin slice, it include the exhibition piece room of body structure, the guide channel being connected to the exhibition piece chamber interior is extended on the side wall of the exhibition piece room, the guide channel is provided with flow-stopping plate far from one end of exhibition piece room, and the guide channel is arranged along length direction opening；The exhibition piece interior is filled with the liquid for impregnating paraffin section；It cuts the slice formed to flow into exhibition piece interior by guiding groove and be immersed in liquid, improves paraffin section collection efficiency and simultaneously optimize experiment flow, to promote this paraffin section technology.
Description
Technical field
The utility model relates to biology and clinical experiment devices, and in particular to a kind of automatic exhibition of Portable paraffin slice
Piece machine.
Background technique
Paraffin section technology is classical in Histological research and most common technology, paraffin section technology combine various biologies
Chemical technology, can qualitative positioning study the presence and distribution of certain substance, be widely used in Neurobiology, group
Knit the research in the fields such as morphology.
But the step of prior paraffin microtomy, is very complicated, needs to be dehydrated by fixation, embed, slice opens up piece,
Fish out a series of processes such as piece.Although the autotechnicon for occurring some business now can simplify early period, tissue is fixed,
Dehydration, the operating process (patent No.: ZL 201410574514.8) of embedding, but in the next steps, the paraffin piece that will be cut out
Still need from the process that knife rest is transferred to tissue exhibition piece machine (patent No.: ZL 201520924730.0) exhibition piece and fishes out piece artificial
Processing.However, the technology is more demanding for the qualification of operator, and especially in air dried environment, paraffin item
Band is extremely easy to happen curling and breakage because of factors such as electrostatic.We queried relevant product on the market and have announced special
Benefit discovery, contrast piece 1 (patent No.: ZL 93108158.0) are related to fluid and drive to flatten the device of paraffin section, can be larger
The reduction slice curling of degree and damaged situation.But the aufbauprinciple and sink volume because of contrast piece 1 are limited, and are relatively suitble to
The a small number of slices of cutting, if needing to biological sample serial section, because waiting the continuous pause caused by opening up piece to will affect slice
Efficiency.For this defect, the volume that contrast piece 2 (patent No.: ZL 2,015 2 0410144.4) increases sink, same
In the case that sample can reduce tissue curling and breakage, serial section can be carried out to sample, the paraffin piece into drift piece room exist
Exhibition piece is completed while slice, reduces pause, further improves the efficiency and quality of paraffin section.But in practical application
During, there is also some defects for contrast piece 2, such as too slow with business drift piece machine heat temperature raising, can not intuitively show water
The actual temperature in face；Its inclined-plane interface and knife rest use glass glue connection, and needs at least time half a day, dress is fully cured in glass cement
It unloads relevant apparatus relatively to waste time, and contrast piece 2 once forms the business paraffin slicing machine for only adapting to single model, it is existing
There are improvements for technology.
Utility model content
In order to further increase the efficiency and optimization experiment flow of paraffin section, so that this paraffin section technology is promoted, this
Utility model proposes a kind of automatic exhibition piece machine of Portable paraffin slice.
In order to achieve the above objectives, the technical solution of the utility model is as follows: a kind of automatic exhibition piece machine of Portable paraffin slice,
Include the exhibition piece room of body structure, the guide channel being connected to the exhibition piece chamber interior be extended on the side wall of the exhibition piece room,
The guide channel is provided with flow-stopping plate far from one end of exhibition piece room, and the guide channel is provided with open setting along length direction
Opening；The exhibition piece interior is filled with the liquid for paraffin section expansion.
The utility model is further arranged to: temperature controlled compartment, setting in the temperature controlled compartment are provided with below the exhibition piece room
There is the silica gel heating plate for fitting in exhibition piece room bottom, the silica gel heating plate is connected with power supply and temperature control device.
The utility model is further arranged to: the exhibition piece interior is provided with temperature sensor, the temperature sensor with
Temperature control device connection.
The utility model is further arranged to: the bottom for stating exhibition piece room is the stainless steel plate for capableing of quick conductive.
The utility model is further arranged to: the bottom of the temperature controlled compartment is provided with the stabilizer blade of adjustment height.
The utility model is further arranged to: the micro- suction glue-line of nanometer is provided on the flow-stopping plate.
In conclusion the utility model has the effect that
1, the micro- material inhaled glue and adsorb smooth object using the small sucker on its surface of nanometer, convenient for portable
Paraffin section open up automatically piece machine be docked to slicer process it is very convenient, need to stay overnight adhesion by original, become with viscous
With with the operating time of experiment is greatly saved；
2, it is quickly increased by silica gel heating plate and stainless steel plate with the use of guarantee water temperature, the time for waiting water temperature to rise
It is obviously shortened, improves conventional efficient；
3, being used cooperatively by temperature sensor and temperature control device, which can make to open up the water in piece case, is maintained at certain stabilization
In section；
4, the adjustable support leg of the setting below temperature controlled compartment can adjust the height of guide channel, to adapt to the business of different model
Paraffin slicing machine expands the use scope of the automatic exhibition piece machine of Portable paraffin slice
Detailed description of the invention
It, below will be to embodiment or the prior art in order to illustrate more clearly of the technical scheme in the embodiment of the utility model
Attached drawing needed in description is briefly described.
Fig. 1 is a kind of overall structure diagram of the automatic exhibition piece machine of Portable paraffin slice.
In figure: 1, opening up piece room；11, stainless steel plate；2, guide channel；21, flow-stopping plate；22, it is open；3, temperature controlled compartment；31, silica gel
Heating plate；32, temperature control device；33, power supply；4, stabilizer blade；5, temperature sensor；6, the micro- suction glue-line of nanometer.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawing.
As shown in Figure 1, a kind of automatic exhibition piece machine of Portable paraffin slice, includes the exhibition piece room 1 of body structure, opens up piece room
The guide channel 2 being connected to inside exhibition piece room 1 is extended on 1 side wall, guide channel 2 is provided with baffle far from one end of exhibition piece room 1
Plate 21, guide channel 2 are provided with the opening 22 of open setting along length direction；Exhibition is filled in piece room 1 for paraffin section
The liquid of expansion.
Further, the lower section for opening up piece room 1 is provided with temperature controlled compartment 3, is provided in temperature controlled compartment 3 and fits in exhibition 1 bottom of piece room
Silica gel heating plate 31, silica gel heating plate 31 is connected with power supply 33 and temperature control device 32.
Further, it opens up in piece room 1 and is provided with temperature sensor 5, temperature sensor 5 is connect with temperature control device 32.
Further, the bottom for opening up piece room 1 is the stainless steel plate 11 for capableing of quick conductive.
Further, the bottom of temperature controlled compartment 3 is provided with the stabilizer blade 4 of adjustment height.
Further, the micro- suction glue-line 6 of nanometer is provided on flow-stopping plate 21.
Specific implementation process:
When applied to paraffin slicing machine, the height of adjustment stabilizer blade 4 first makes to open up piece machine in suitable height, is guaranteeing to keep off
In the case where the micro- suction glue-line 6 of nanometer and holder surface cleaning drying in flowing plate 21, the micro- suction glue-line 6 of nanometer, which is firmly pressed, makes it
It is adsorbed on the knife rest of business paraffin slicing machine；Pure water is injected into guide channel 2 and exhibition piece room 1, makes water surface elevation and business
The blade top edge of paraffin slicing machine is concordant；33 are turned on the power switch to power on, by the temperature setting of temperature control device be 38~
40 DEG C, after the actual water temperature to be seen to temperature sensor 5 reaches setting value, it can start to be sliced；Quotient need to be only rotated when slice
The handle of industry paraffin slicing machine, the paraffin section cut out will swim in automatically on the water surface of guide channel 2, and with slice into
Row, the paraffin band that serial section is cut out can enter exhibition piece room 1 through guide channel 2, after paraffin section is fully deployed on the water surface,
I.e. available glass slide carries out fishing piece.
Application examples:
The complete sequence of various tissue specimens is sliced:
We are with the hippocampus and kidney of adult mice, the brain of embryonic-period mice and the eyeball tissue of Adult Zebrafish
Example selects business paraffin slicing machine Leica MR2235, selects blade SAKURA Accu Edge 4689, to above-mentioned tissue point
Coronal-plane complete sequence serial section, exhibition piece and fishing piece have not been carried out with the present invention and business water-bath booth piece machine.
By Jing Guo paraffin embedding tissue samples (hippocampus and kidney of adult mice, the brain of embryonic-period mice and at
The eyeball tissue of year zebra fish) it is fixed on the specimen holder of business paraffin slicing machine.30 μm are set by slice thickness, is quickly turned
The handle of dynamic business paraffin slicing machine occurs until first with organized paraffin section.Then according to different tissue need
It asks and adjusts slice thickness (hippocampus and kidney of adult mice, 10 μm of the brain of embryonic-period mice, the eyeball group of Adult Zebrafish
Knit 4 μm), uniform rotation business paraffin section cleaner handgrip carries out complete sequence serial section, and the paraffin sector-meeting cut out is swum in automatically
In guide channel 2, and with the progress of slice, enter exhibition piece room 1 through guide channel 2.Etc. be introduced into exhibition piece room 1 paraffin section in water
After being fully deployed on face, stop operating handle, with tweezers by the separation of the paraffin band of suitable length (hippocampus of adult mice and
Kidney, every 4 one group of the brain of embryonic-period mice, every 10 1 group of Adult Zebrafish eyeball), and will be after separation with glass slide
Paraffin segment carefully fishes out piece.It repeats the above process, until fishing out the last a piece of paraffin section with tissue out of exhibition piece room 1
It rises.Glass slide is then put into 42 DEG C of baking ovens to dry glass slide.
In the application example, we cut different tissues with by the improved dicing method of the present invention and tradition respectively
Piece method is compared, and as shown in Table 1, statistical analysis, all improved methods of test sample carry out single tissue complete
Serviceability rate (the intact number of slices of the quality that slice serviceability rate=singulation sample obtains/single mark is sliced after Sequentially continuous slice
Originally the slice sum cut out) it is obviously improved compared with conventional method.In addition, as shown in Table 2, with improved method to all surveys
This progress of sample individually organizes the time spent by complete sequence serial section to be also significantly lower than conventional method.Therefore, through the present invention
Improved paraffin section technology high degree simplifies traditional paraffin section technology, the paraffin for making slice efficiency and cutting out
The quality of piece is obviously improved, generally applicable to various biological tissues.
Portable paraffin section open up automatically piece machine device using temperature control device 32 realize to exhibition 1 water temperature of piece room accurate control
System keeps exhibition piece temperature more stable；It bonds, makes with the micro- knife rest for inhaling glue-line 6 and business paraffin slicing machine of nanometer at guiding groove 2
The disassembly of the invention is more flexible；The stabilizer blade 4 of 3 bottom of temperature controlled compartment can suitably adjust height of the invention, allow the present invention with
Various types of business paraffin slicing machine docking.Therefore, the present invention is greatly improved the practicability of existing apparatus, thus into one
Step improves the quality and efficiency of paraffin section.
Table one is using conventional method compared with through the improved method slice serviceability rate of the invention
| Conventional method | Improved method | P value | |
| Adult mice hippocampus obtains the ratio (n=3) of intact slice | 91.04 ± 1.429% | 97.63 ± 0.4864% | 0.0120<0.05 |
| Adult mice kidney obtains the ratio (n=3) of intact slice | 92.30 ± 0.5689% | 95.99 ± 0.7055% | 0.0153<0.05 |
| Fetal mice brain obtains the ratio (n=3) of intact slice | 90.67 ± 1.549% | 97.01 ± 0.2816% | 0.0158<0.05 |
| Adult Zebrafish eyeball obtains the ratio (n=4) of intact slice | 91.27 ± 0.9734% | 96.32 ± 0.7217% | 0.0059<0.01 |
Table two is using conventional method compared with through the invention improved method complete sequence slice time
| Conventional method | Improved method | P value | |
| Adult mice hippocampus complete sequence is sliced time (min) (n=3) | 126.0±3.606 | 79.67±1.856 | 0.0003<0.001 |
| Adult mice kidney complete sequence is sliced time (min) (n=3) | 66.67±1.764 | 41.33±2.186 | 0.0008<0.001 |
| Fetal mice brain complete sequence is sliced time (min) (n=3) | 99.67±3.480 | 52.33±4.333 | 0.0010<0.01 |
| Adult Zebrafish eyeball complete sequence is sliced time (min) (n=4) | 48.00±2.160 | 30.75±1.797 | 0.0009<0.001 |
It should be pointed out that for those of ordinary skill in the art, before not departing from the concept of the present invention
It puts, various modifications and improvements can be made, and these are all within the protection scope of the present invention.
Claims (6)
1. a kind of automatic exhibition piece machine of Portable paraffin slice, which is characterized in that it include the exhibition piece room (1) of body structure, it is described
The guide channel (2) for being extended on the side wall of piece room (1) and being connected to inside exhibition piece room (1) is opened up, the guide channel (2) is far from exhibition
One end of piece room (1) is provided with flow-stopping plate (21), and the guide channel (2) is provided with the opening of open setting along length direction
(22)；The liquid for paraffin section expansion is filled in exhibition piece room (1).
2. a kind of automatic exhibition piece machine of Portable paraffin slice according to claim 1, which is characterized in that the exhibition piece room
(1) it is provided with below temperature controlled compartment (3), the silica gel heating plate for fitting in exhibition piece room (1) bottom is provided in the temperature controlled compartment (3)
(31), the silica gel heating plate (31) is connected with power supply (33) and temperature control device (32).
3. a kind of automatic exhibition piece machine of Portable paraffin slice according to claim 2, which is characterized in that the exhibition piece room
(1) it is provided with temperature sensor (5) in, the temperature sensor (5) connect with temperature control device (32).
4. a kind of automatic exhibition piece machine of Portable paraffin slice according to claim 2 or 3, which is characterized in that the exhibition piece
The bottom of room (1) is the stainless steel plate (11) for capableing of quick conductive.
5. a kind of automatic exhibition piece machine of Portable paraffin slice according to claim 2, which is characterized in that the temperature controlled compartment
(3) bottom is provided with the stabilizer blade (4) of adjustment height.
6. a kind of automatic exhibition piece machine of Portable paraffin slice according to claim 1, which is characterized in that the flow-stopping plate
(21) the micro- suction glue-line (6) of nanometer is provided on.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822020676.3U CN209673460U (en) | 2018-11-30 | 2018-11-30 | A kind of automatic exhibition piece machine of Portable paraffin slice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822020676.3U CN209673460U (en) | 2018-11-30 | 2018-11-30 | A kind of automatic exhibition piece machine of Portable paraffin slice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209673460U true CN209673460U (en) | 2019-11-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822020676.3U Expired - Fee Related CN209673460U (en) | 2018-11-30 | 2018-11-30 | A kind of automatic exhibition piece machine of Portable paraffin slice |
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| CN (1) | CN209673460U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111307551A (en) * | 2020-03-24 | 2020-06-19 | 宁波海关技术中心 | Multi-functional pathological tissue paraffin section processing apparatus |
-
2018
- 2018-11-30 CN CN201822020676.3U patent/CN209673460U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111307551A (en) * | 2020-03-24 | 2020-06-19 | 宁波海关技术中心 | Multi-functional pathological tissue paraffin section processing apparatus |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191122 Termination date: 20201130 |