CN205710707U - A kind of rotary negative-pressure adsorption gel sickle of band ultraviolet light - Google Patents

A kind of rotary negative-pressure adsorption gel sickle of band ultraviolet light Download PDF

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Publication number
CN205710707U
CN205710707U CN201620410903.1U CN201620410903U CN205710707U CN 205710707 U CN205710707 U CN 205710707U CN 201620410903 U CN201620410903 U CN 201620410903U CN 205710707 U CN205710707 U CN 205710707U
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CN
China
Prior art keywords
sickle
cutter head
sleeve
withdrawing
ultraviolet light
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201620410903.1U
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Chinese (zh)
Inventor
李亚琴
赵贵森
戚恒亮
陈炯
艾红伟
詹飞
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Henan University of Science and Technology
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Henan University of Science and Technology
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Publication date
Application filed by Henan University of Science and Technology filed Critical Henan University of Science and Technology
Priority to CN201620410903.1U priority Critical patent/CN205710707U/en
Application granted granted Critical
Publication of CN205710707U publication Critical patent/CN205710707U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

A kind of rotary negative-pressure adsorption gel sickle of band ultraviolet light, including base, the ultraviolet light being arranged in base, the support bar being arranged on base and sickle, sickle is rotated by fixture and is fixed on above object stage, the centre position of object stage is provided with transparent area, the both sides of transparent area are respectively arranged with molding cutter head rest area, gel piece rest area and discarded cutter head rest area, sickle includes that one end has the sleeve of air bag, the withdrawing axle that is slidably arranged in sleeve and be slidably arranged in the cutter shaft in withdrawing axle, one end of cutter shaft protrudes from sleeve and is connected with molding cutter head.This sickle can cut and shift the gel piece of different size with fast and easy, reduce the injury under ultraviolet irradiates of skin and nucleic acid, and the gel piece of cutting can be drawn accurately, utilize the effect of negative pressure gasbag, gel piece is made to be fixed in cutter head, it is to avoid but gel piece is cut the risk being the most disposably transferred.

Description

A kind of rotary negative-pressure adsorption gel sickle of band ultraviolet light
Technical field
This utility model relate to ultraviolet gel reclaim, purification art, a kind of band ultraviolet light rotary Negative-pressure adsorption gel sickle.
Background technology
Agarose gel electrophoresis technology is widely used in separation and the purification of molecular biology nucleic acid.In nucleic acid purification process In, the inevitable operating procedure of laboratory staff cuts glue exactly.At present in most Molecular Biology Lab, work Personnel are repeatedly to cut by blade, then take out gel piece.Carry out under ultra violet lamp owing to taking glue operation, and Taking the glue time at 3 ~ 5 minutes, such long campaigns takes glue operation, stands ultra violet lamp and can damage human skin tissue, And easily make the nucleic acid framework in gel be destroyed.
Utility model content
For solving ultraviolet direct irradiation when staff in prior art directly takes blade to carry out cutting and take out blob of viscose The problem of nucleic acid framework that skin-on-skin causes damage and is easily destroyed in gel, it is purple that this utility model provides a kind of band The rotary negative-pressure adsorption gel sickle of light modulation, this rotary negative-pressure adsorption sickle can cut with fast and easy and shift not The gel piece of same specification, reduces the injury under skin exposure and the destruction of nucleic acid framework, and is capable of the disposable of cutter head Install, cut and draw the gel piece after cutting accurately, and purpose blob of viscose is transferred to gel piece rest area, cutter will be polluted Head is offloaded to discarded cutter head rest area, it is achieved quickly collects purpose blob of viscose and prevents the purpose polluted.Originally glue device is cut at object stage On four regions are set, be molding cutter head rest area, transparent area, gel piece rest area and discarded cutter head rest area respectively, the end of at Seat ultraviolet light installed above, and at ultraviolet light convex lens sheet installed above.During once cutting glue, gel piece is placed on Light district, rotation is fixed on the fixture above support bar and installs suitable cutter head in cutter head district, continues rotary cutter, at transparent area Select purpose blob of viscose, object stage has the open area matched with uviol lamp, convex lens, opens uviol lamp on and off switch, adjust Joint focusing knob, can be clearly observed purpose fragment region, it addition, there is the support of base and object stage so that cut Glue process is safer, quick so that cut glue efficiency increase substantially cut the glue time shorten, ultraviolet light according to the time shorten, target Segment area locking is accurately so that Nucleic acid quality is high, little to human injury, and the operating time shortens, and closes ultraviolet light, shifts blob of viscose To blob of viscose rest area, continue rotary cutting glue device and pollute cutter head to discarded cutter head rest area, unloading, complete a cutting process.
The technical scheme that this utility model is used by the above-mentioned technical problem of solution is: the rotary of a kind of band ultraviolet light is born Pressure attractive gel sickle, the ultraviolet light including base, being arranged in base, the support bar being arranged on base and sickle, Wherein, the upper and lower of support bar is respectively arranged with Turnable fixture and object stage, and described sickle passes through Turnable fixture Being fixed on above object stage, the centre position of object stage is provided with through transparent area, so that the light of ultraviolet light leads in base It is radiated at after crossing a convex lens sheet at transparent area, is respectively arranged with molding cutter head rest area in the both sides of transparent area, gel piece is put Putting district and discarded cutter head rest area, described sickle includes the sleeve that one end has air bag, the withdrawing being slidably arranged in sleeve Axle and be slidably arranged in the cutter shaft in withdrawing axle, one end of cutter shaft protrudes from sleeve and is positioned at air bag, and the other end also highlights Be connected in sleeve and with molding cutter head, in cutter shaft, be provided with communicated air bags and the gas port of molding cutter head, the inwall of withdrawing axle and It is respectively arranged with chuck I and chuck II on outer wall, and chuck I coordinates with the chuck III arranged on cutter shaft outer wall, so that cutter shaft band Dynamic withdrawing axle moves downward or withdrawing axle band movable arbor moves upward, and withdrawing axle can not move downward by band movable arbor;Described card Be provided with back-moving spring on platform II so that after cutter shaft is pressed, withdrawing axle can under back-moving spring elastic force effect drives cutter shaft Setting back, described forming cutter centriciput has the cavity accommodating gel piece, and this cavity is connected with air bag by gas port;Institute Stating and be provided with card pressure button on withdrawing axle, with when pressing card pressure button, card pressure button drives withdrawing axle to move downward along cutter shaft, And molding cutter head is backed off from the end of cutter shaft.
Described cutter shaft is positioned on one end of air bag and is provided with positive stop, after limiting cutter shaft and the pressing of molding cutter head Extreme position.
The distance of described positive stop and sleeve ends is more than the thickness of molding cutter head.
Described fixture is movably arranged on support bar, to realize the regulation of sickle height.
Described transparent area is strip, and is positioned on the line of molding cutter head and convex lens sheet.
On described base, threads turn being provided with focusing sleeve, convex lens sheet is arranged on the top of this focusing sleeve, To realize the focal length by rotating focusing sleeve regulation convex lens sheet.
Between described molding cutter head and the end of sleeve, there is gap.
Object stage in this utility model is made up of four parts, is cutter head rest area, transparent area, gel piece rest area respectively With discarded cutter head rest area, transparent area is strip, and is positioned on the line of molding cutter head and convex lens sheet, and cutter head district is at transparent area Left side, gel rest area, discarded cutter head rest area are on the right side of transparent area.
In this utility model, ultraviolet light, convex lens sheet, object stage transparent area are in same range of observation, and ultraviolet light is fixed In chassis interior, being connected with convex lens sheet by cavity, ultraviolet light is connected with power supply, when needing, turns on the power, and ultraviolet light is bright, The not used time, close power supply, it is also possible to by regulation focusing knob, the light changing ultraviolet light is strong and weak.
Beneficial effect: this utility model compared with prior art, has the advantage that
1 by arranging negative pressure gasbag in sleeve one end, it is possible to ensures disposably cut and draw purpose gel piece, it is to avoid two The secondary glue that takes, reduces nucleic acid structure and destroys, meanwhile, it is capable to utilize gas port to be drawn by gel piece and blowout;
Sickle is fixed on base by 2, equipped with adjustable ultraviolet device below base, it is possible to achieve quickly, accurately Cut blob of viscose, and the infringement of less contact skin;
3 simple in construction, cost of manufacture is low, cuts glue device and fixes flexibly, is convenient for changing and safeguards;
One end of 4 cutter shafts protrudes from sleeve, and threadeds with the employing of molding cutter head, is conducive to changing molding as required Cutter head, it is to avoid pollute;
5 sleeves are connected by back-moving spring with cutter shaft, thus are capable of automatically reseting after by crush-cutting glue;
6 ultraviolet lights are fixed in base, and with convex lens sheet, object stage flexible connectivity, can quickly, accurately adjust and cut glue Scheme, fast searching and change purpose blob of viscose;
The 7 flexible scalable of convex lens sheet, after selected purpose blob of viscose, can reduce the quantity of illumination, alleviate nucleic acid framework destroy and Damage to person.
It is provided with four regions on 8 object stages, is cutter head rest area, transparent area, gel piece rest area and discarded cutter respectively Head rest area, it is possible to realize cutting disposably installing cutter head, unloading cutter head, cut glue, transfer adhesive of glue device by flexible rotating fixture The purpose of block.There is a disposable flow process, time-consuming, it is to avoid to pollute.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation of sickle;
Fig. 3 is the fit structure schematic diagram of cutter shaft and withdrawing axle;
Reference: 1, sleeve, 2, withdrawing axle, 3, cutter shaft, 4, gas port, 5, positive stop, 6, air bag, 7, card pressure presses Button, 8, back-moving spring, 9, molding cutter head, 10, ultraviolet light, 11, focusing sleeve, 12, convex lens sheet, 13, transparent area, 14, loading Platform, 15, fixture, 16, molding cutter head rest area, 17, support bar, 18, gel piece rest area, 19, discarded cutter head rest area, 20, Base.
Detailed description of the invention
As it can be seen, the rotary negative-pressure adsorption gel sickle of a kind of band ultraviolet light, including base 20, it is arranged on base Ultraviolet light 10 in 20, the support bar 17 being arranged on base 20 and sickle, wherein, the upper and lower of support bar 17 is respectively Being provided with Turnable fixture 15 and object stage 14, described sickle is fixed on above object stage 14 by Turnable fixture 15, carries The centre position of thing platform 9 is provided with through transparent area 13, so that the light of ultraviolet light 10 is by a convex lens sheet in base 20 It is radiated at transparent area 13 after 12, is respectively arranged with molding cutter head rest area 16, gel piece rest area in the both sides of transparent area 13 18 and discarded cutter head rest area 19, described sickle includes that one end has the sleeve 1 of air bag 6, is slidably arranged in moving back in sleeve 1 Cutter shaft 2 and the cutter shaft 3 being slidably arranged in withdrawing axle 2, one end of cutter shaft 3 protrudes from sleeve 1 and is positioned at air bag 6, another End also protrudes from sleeve 1 and is connected with molding cutter head 9, is provided with the gas port 5 of communicated air bags 6 and molding cutter head 9 in cutter shaft 3, It is respectively arranged with chuck I 201 and chuck II 202 on the inner and outer wall of withdrawing axle 2, and chuck I 201 sets on cutter shaft 3 outer wall The chuck III 301 put coordinates, so that cutter shaft 3 drives withdrawing axle 2 to move downward or withdrawing axle 2 band movable arbor 3 moves upward, and moves back Cutter shaft 2 can not move downward by band movable arbor 3;It is provided with back-moving spring 8 in described chuck II 202, so that after cutter shaft 3 is pressed, Withdrawing axle 2 can set back by cutter shaft 3 under back-moving spring 8 elastic force effect drives, and has receiving solidifying in the middle part of described molding cutter head 9 The cavity of blob of viscose, and this cavity connected with air bag 6 by gas port 4;Card pressure button 7 it is provided with, to press on described withdrawing axle 2 During pressure card pressure button 7, card pressure button 7 drives withdrawing axle 2 to move downward along cutter shaft 3, and by molding cutter head 9 from the end of cutter shaft 3 Back off.
It is more than basic embodiment of the present utility model, can further be limited, optimize and change on the basis of above Enter:
As, described cutter shaft 3 is positioned on one end of air bag 6 and is provided with positive stop 5, to limit cutter shaft 3 and molding cutter head 9 Extreme position after pressing;
Further, the distance of described positive stop 5 and sleeve 1 end is more than the thickness of molding cutter head 9;
For another example, between described molding cutter head 7 and the end of sleeve 1, there is gap, in order to change molding cutter head 7, molding Cutter head 7 can be customized into variously-shaped and size as required;
For another example, described fixture 15 is movably arranged on support bar 17, to realize the regulation of sickle height;
For another example, described transparent area 13 is strip, and is positioned on the line of molding cutter head 9 and convex lens sheet 12, object stage 14 And between convex lens sheet 12, there is gap;
Finally, described base 20 being provided with focusing sleeve 11 by threads turn, convex lens sheet 12 is arranged on this focusing The top of sleeve 11, to realize regulating the focal length of convex lens sheet 12 by rotating focusing sleeve 11.
Using method of the present utility model is: rotary cutter installs cutter head to cutter head rest area 16, is put in by gel piece Transparent area 13, opens ultraviolet violet light switch, regulation focusing sleeve 11, selects rapidly DNA band to be cut under ultra violet lamp, Holding sleeve 1, thumb is placed on air bag 6, by pressuring gasbag under thumb 6 and cutter shaft 3 top contact, is hung down with gel by molding cutter head 9 Directly, it is placed on above gel, the position at alignment DNA band place, continue lower pressuring gasbag 6, in gel piece meeting insert molding cutter head 9, Unclamping thumb, rolling clamp 15 to gel piece rest area 18, now gel piece is acted on by air negative pressure, in moving process Being difficult to slide, continue lower pressuring gasbag 6, in air bag 6, remaining air arrives gel piece along gas port 4, and gel piece is at air-flow Forming cutter head 9 is skidded off under promotion;Unclamping air bag 6 and cutter shaft 3 top, cutter shaft 3 is homing under the effect of back-moving spring 8, empty Gas is full of air bag 6 along gas port 4, rolling clamp 15 to discarded cutter head rest area 19, pressing card pressure button 7, unloads forming cutter 9.Rotate to molding cutter head rest area 16 installation forming cutter head 9, prepare to cut next time, after the cutting of all purpose fragments Closedown uviol lamp.

Claims (7)

1. the rotary negative-pressure adsorption gel sickle of a band ultraviolet light, it is characterised in that: include base (20), be arranged on the end Ultraviolet light (10), the support bar (17) being arranged on base (20) and sickle in seat (20), wherein, support bar (17) upper Portion and bottom are respectively arranged with Turnable fixture (15) and object stage (14), and described sickle is fixed by Turnable fixture (15) In object stage (14) top, the centre position of object stage (14) is provided with through transparent area (13), so that purple in base (20) The light of light modulation (10) is radiated at transparent area (13) place by after a convex lens sheet (12), sets respectively in the both sides of transparent area (13) Being equipped with molding cutter head rest area (16), gel piece rest area (18) and discarded cutter head rest area (19), described sickle includes one Hold sleeve (1), the withdrawing axle (2) being slidably arranged in sleeve (1) with air bag (6) and be slidably arranged in withdrawing axle (2) In cutter shaft (3), one end of cutter shaft (3) protrudes from sleeve (1) and is positioned at air bag (6), the other end also protrude from sleeve (1) and It is connected with molding cutter head (9), in cutter shaft (3), is provided with the gas port (4) of communicated air bags (6) and molding cutter head (9), withdrawing axle (2) chuck I (201) and chuck II (202) it are respectively arranged with on inner and outer wall, and chuck I (201) and cutter shaft (3) outer wall The chuck III (301) of upper setting coordinates, so that cutter shaft (3) drives withdrawing axle (2) to move downward or withdrawing axle (2) band movable arbor (3) move upward, and withdrawing axle (2) can not move downward by band movable arbor (3);It is provided with reset bullet in described chuck II (202) Spring (8), so that after cutter shaft (3) is pressed, withdrawing axle (2) can recover by cutter shaft (3) under back-moving spring (8) elastic force effect drives In situ, described molding cutter head (9) middle part has the cavity accommodating gel piece, and this cavity is by gas port (4) with air bag (6) even Logical;Being provided with card pressure button (7) on described withdrawing axle (2), with when pressing card pressure button (7), card pressure button (7) drives withdrawing Axle (2) moves downward along cutter shaft (3), and is backed off from the end of cutter shaft (3) by molding cutter head (9).
The rotary negative-pressure adsorption gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterised in that: institute State cutter shaft (3) to be positioned on one end of air bag (6) and be provided with positive stop (5), press limiting cutter shaft (3) and molding cutter head (9) Extreme position after pressure.
The rotary negative-pressure adsorption gel sickle of a kind of band ultraviolet light the most according to claim 2, it is characterised in that: institute State the distance thickness more than molding cutter head (9) of positive stop (5) and sleeve (1) end.
The rotary negative-pressure adsorption gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterised in that: institute State fixture (15) to be movably arranged on support bar (17), to realize the regulation of sickle height.
The rotary negative-pressure adsorption gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterised in that: institute Stating transparent area (13) is strip, and is positioned on the line of molding cutter head (9) and convex lens sheet (12).
The rotary negative-pressure adsorption gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterised in that: institute Stating, on base (20), threads turn is provided with focusing sleeve (11), convex lens sheet (12) is arranged on this focusing sleeve (11) Top, to realize the focal length by rotating focusing sleeve (11) regulation convex lens sheet (12).
The rotary negative-pressure adsorption gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterised in that: institute State, between the end of molding cutter head (9) and sleeve (1), there is gap.
CN201620410903.1U 2016-05-09 2016-05-09 A kind of rotary negative-pressure adsorption gel sickle of band ultraviolet light Expired - Fee Related CN205710707U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201620410903.1U CN205710707U (en) 2016-05-09 2016-05-09 A kind of rotary negative-pressure adsorption gel sickle of band ultraviolet light

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201620410903.1U CN205710707U (en) 2016-05-09 2016-05-09 A kind of rotary negative-pressure adsorption gel sickle of band ultraviolet light

Publications (1)

Publication Number Publication Date
CN205710707U true CN205710707U (en) 2016-11-23

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108330223A (en) * 2018-01-10 2018-07-27 宁波德深机械设备有限公司 A kind of cutter device
CN112432989A (en) * 2020-10-29 2021-03-02 北京擎科生物科技有限公司 Rubber cutting instrument

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108330223A (en) * 2018-01-10 2018-07-27 宁波德深机械设备有限公司 A kind of cutter device
CN112432989A (en) * 2020-10-29 2021-03-02 北京擎科生物科技有限公司 Rubber cutting instrument

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C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20161123

Termination date: 20170509