CN205616873U - Take negative -pressure adsorption type gel exciser ware of purple light lamp - Google Patents
Take negative -pressure adsorption type gel exciser ware of purple light lamp Download PDFInfo
- Publication number
- CN205616873U CN205616873U CN201620410949.3U CN201620410949U CN205616873U CN 205616873 U CN205616873 U CN 205616873U CN 201620410949 U CN201620410949 U CN 201620410949U CN 205616873 U CN205616873 U CN 205616873U
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- sleeve
- sickle
- ultraviolet light
- cutter head
- negative
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Abstract
The utility model provides a take negative -pressure adsorption type gel exciser ware of purple light lamp, includes the base, sets up purple light lamp, bracing piece and the cutterbar of setting on the base in the base, and the upper portion and the lower part of bracing piece are provided with anchor clamps and objective table respectively, and the cutterbar passes through anchor clamps to be fixed in the objective table top, and the intermediate position of objective table is provided with the light zone that passes through who link up to the light that makes purple light lamp in the base shines after through a convex lens piece and is passing through light zone department, the cutterbar includes that one end has the sleeve and the arbor of setting in the sleeve that slide of gasbag, and the one end salient of arbor is connected in the sleeve and with the shaping tool bit. This cutterbar can make things convenient for the fly -cutting and shift the gel piece of different specifications, reduces skin and the injury of nucleic acid under the ultraviolet irradiation to the gel piece of absorption cutting that can be accurate utilizes the effect of negative pressure gasbag, make the gel piece fix in the tool bit, but it does not have the risk that is once only shifted to avoid the gel piece to be cut.
Description
Technical field
This utility model relates to the recovery of ultraviolet gel, purification art, the negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light.
Background technology
Agarose gel electrophoresis technology is widely used in separation and the purification of molecular biology nucleic acid.During nucleic acid purification, the inevitable operating procedure of laboratory staff cuts glue exactly.At present in most Molecular Biology Lab, staff is repeatedly to cut by blade, then takes out gel piece.Carrying out under ultra violet lamp owing to taking glue operation, and take the glue time at 3 ~ 5 minutes, such long campaigns takes glue operation, stands ultra violet lamp and can damage human skin tissue, and easily makes the nucleic acid framework in gel be destroyed.
Utility model content
The problem of the nucleic acid framework caused damage for ultraviolet direct irradiation skin-on-skin when staff directly carries out cutting and take out blob of viscose by blade in solution prior art and be easily destroyed in gel, this utility model provides the negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light, this negative-pressure adsorption-type sickle can cut and shift the gel piece of different size with fast and easy, reduce the injury under skin exposure and the destruction of nucleic acid framework, and can cut and draw the gel piece after cutting accurately.Originally cut glue device, at submounts, uviol lamp is installed, and at uviol lamp convex lens sheet installed above, glue cut by needs when, gel piece is placed on object stage, have on object stage and uviol lamp, the open area that convex lens matches, open uviol lamp on and off switch, regulation focusing knob, purpose fragment region can be clearly observed, additionally, there is the support of base and object stage, make to cut glue process safer, quickly, make to cut glue efficiency to increase substantially and cut the glue time and shorten, uviol lamp shortened according to the time, the locking of purpose segment area is accurately, make Nucleic acid quality high, little to human injury, operating time shortens.Utilize the effect of negative pressure gasbag, make gel piece be fixed in cutter head, it is to avoid but gel piece is cut and is not disposably shifted and suffer contaminated risk.
nullThe technical scheme that this utility model is used by the above-mentioned technical problem of solution is: the negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light，Including base、The ultraviolet light being arranged in base、The support bar being arranged on base and sickle，Wherein，The upper and lower of support bar is respectively arranged with fixture and object stage，Described sickle is fixed on above object stage by fixture，The centre position of object stage is provided with through transparent area，So that the light of ultraviolet light is radiated at transparent area by after a convex lens sheet in base，Described sickle includes the sleeve that one end has air bag and the cutter shaft being slidably arranged in sleeve，One end of cutter shaft protrudes from sleeve and is positioned at air bag，The other end also protrudes from sleeve and is connected with molding cutter head，Communicated air bags and the gas port of molding cutter head it is provided with in cutter shaft，Cutter shaft is connected in sleeve by a back-moving spring，So that cutter shaft and molding cutter head can set back after being pressed under back-moving spring elastic force effect，Described forming cutter centriciput has the cavity accommodating gel piece，And this cavity is connected with air bag by gas port.
Described cutter shaft is positioned on one end of air bag and is provided with positive stop, with limit cutter shaft and molding cutter head pressing after extreme position.
The distance of described positive stop and sleeve ends is more than the thickness of molding cutter head.
The surface configuration of described sleeve is with handles.
Described cutter shaft is provided with annular boss on one end outer wall of molding cutter head, and described back-moving spring is located on cutter shaft, and one end is fixed on this annular boss, and the other end is fixed on the end of sleeve lining.
Described molding cutter head uses with the end of sleeve threadeds, and has gap between the two, in order to change molding cutter head.
Described fixture is movably arranged on support bar, to realize the regulation of sickle height.
Described transparent area is strip, and is positioned on the line of molding cutter head and convex lens sheet.
In this utility model, ultraviolet light, convex lens sheet, object stage transparent area are in same range of observation, and ultraviolet light is fixed on chassis interior, is connected with convex lens sheet by cavity, ultraviolet light is connected with power supply, when needing, turning on the power, ultraviolet light is bright, the not used time, closedown power supply, it is also possible to by regulation focusing knob, the light changing ultraviolet light is strong and weak.
Beneficial effect: this utility model compared with prior art, has the advantage that
1) by arranging negative pressure gasbag in sleeve one end, it is possible to ensure disposably cut and draw purpose gel piece, it is to avoid secondary takes glue, reduce nucleic acid structure and destroy, meanwhile, it is capable to utilize gas port to be drawn by gel piece and blowout；
2) sickle is fixed on base, equipped with adjustable ultraviolet device below base, it is possible to achieve quickly, accurately cut blob of viscose, and the infringement of less contact skin；
3) simple in construction, cost of manufacture is low, cuts glue device structure and meets organization of human body feature, facilitates taking；
4) one end of cutter shaft protrudes from sleeve, and threadeds with the employing of molding cutter head, is conducive to changing the most as required molding cutter head, it is to avoid pollute；
5) sleeve is connected by back-moving spring with cutter shaft, thus is capable of automatically reseting after by crush-cutting glue；
6) in ultraviolet light is fixed on base, and with convex lens sheet, object stage flexible connectivity, can quickly, accurately adjust glue scheme of cutting, fast searching and change purpose blob of viscose；
7) the flexible scalable of convex lens sheet, after selected purpose blob of viscose, can reduce the quantity of illumination, alleviates nucleic acid framework and destroys and damage to person.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of sickle；
Reference: 1, sleeve, 2, handle, 3, air bag, 4, cutter shaft, 5, gas port, 6, back-moving spring, 7, molding cutter head, 8, positive stop, 9, object stage, 10, convex lens sheet, 11, ultraviolet light, 12, base, 13, support bar, 14, fixture, 15, transparent area.
Detailed description of the invention
nullAs shown in the figure，A kind of negative-pressure adsorption-type gel sickle of band ultraviolet light，Including base 12、The ultraviolet light 11 being arranged in base 12、The support bar 13 being arranged on base 12 and sickle，Wherein，The upper and lower of support bar 13 is respectively arranged with fixture 14 and object stage 9，Described sickle is fixed on above object stage 9 by fixture 14，The centre position of object stage 9 is provided with through transparent area 15，So that the light of ultraviolet light 11 is radiated at transparent area 15 by after a convex lens sheet 10 in base 12，Described sickle includes the sleeve 1 that one end has air bag 3 and the cutter shaft 4 being slidably arranged in sleeve 1，One end of cutter shaft 4 protrudes from sleeve 1 and is positioned at air bag 3，The other end also protrudes from sleeve 1 and is connected with molding cutter head 7，The gas port 5 of communicated air bags 3 and molding cutter head 7 it is provided with in cutter shaft 4，Cutter shaft 4 is connected in sleeve 1 by a back-moving spring 6，So that cutter shaft 4 and molding cutter head 7 can set back after being pressed under back-moving spring 6 elastic force effect，There is in the middle part of described molding cutter head 7 cavity accommodating gel piece，And this cavity is connected with air bag 3 by gas port 5.
It is more than basic embodiment of the present utility model, can further be limited, optimize and improve on the basis of above:
As, described cutter shaft 4 is positioned on one end of air bag 3 and is provided with positive stop 8, with limit cutter shaft 4 and molding cutter head 7 pressing after extreme position；
Further, the distance of described positive stop 8 and sleeve 1 end is more than the thickness of molding cutter head 7；
And for example, the surface configuration with handles 2 of described sleeve 1, to facilitate operator hand-held；
For another example, described cutter shaft 4 is provided with annular boss on one end outer wall of molding cutter head 7, and described back-moving spring 6 is located on cutter shaft 4, and one end is fixed on this annular boss, and the other end is fixed on the end of sleeve 1 inwall；
For another example, described molding cutter head 7 uses with the end of sleeve 1 threadeds, and has gap between the two, in order to changing molding cutter head 7, molding cutter head 7 can be customized into variously-shaped and size as required；
For another example, described fixture 14 is movably arranged on support bar 13, to realize the regulation of sickle height；
Finally, described transparent area 15 is strip, and is positioned on the line of molding cutter head 7 and convex lens sheet 10, has gap between object stage 9 and convex lens sheet 10.
Using method of the present utility model is: be put on object stage by gel piece, open ultraviolet violet light switch, regulation focusing knob, DNA band to be cut is selected rapidly under ultra violet lamp, hold handle 2, thumb is placed on air bag 3, by pressuring gasbag under thumb 3 and cutter shaft 4 top contact, by vertical with gel for molding cutter head 7, it is placed on above gel, the position at alignment DNA band place, continue lower pressuring gasbag 3, in gel piece meeting insert molding cutter head 7, unclamp thumb, gel is moved to target location, now gel piece is acted on by air negative pressure, moving process is difficult to slide, continue lower pressuring gasbag 3, in air bag 3, remaining air arrives gel piece along gas port 5, gel piece skids off forming cutter head 7 under the promotion of air-flow；Unclamping air bag 3 and cutter shaft 4 top, cutter shaft 4 is homing under the effect of back-moving spring 6, and air is full of air bag 3 along gas port 5, prepares to cut next time, closes uviol lamp after the cutting of all purpose fragments.
Claims (8)
- null1. the negative-pressure adsorption-type gel sickle of a band ultraviolet light，It is characterized in that: include base (12)、The ultraviolet light (11) being arranged in base (12)、The support bar (13) being arranged on base (12) and sickle，Wherein，The upper and lower of support bar (13) is respectively arranged with fixture (14) and object stage (9)，Described sickle is fixed on object stage (9) top by fixture (14)，The centre position of object stage (9) is provided with through transparent area (15)，So that the light of base (12) interior ultraviolet light (11) is radiated at transparent area (15) place by after a convex lens sheet (10)，Described sickle includes the sleeve (1) that one end has air bag (3) and the cutter shaft (4) being slidably arranged in sleeve (1)，One end of cutter shaft (4) protrudes from sleeve (1) and is positioned at air bag (3)，The other end also protrudes from sleeve (1) and is connected with molding cutter head (7)，The gas port (5) of communicated air bags (3) and molding cutter head (7) it is provided with in cutter shaft (4)，Cutter shaft (4) is connected in sleeve (1) by a back-moving spring (6)，So that cutter shaft (4) and molding cutter head (7) can set back after being pressed under back-moving spring (6) elastic force effect，Described molding cutter head (7) middle part has the cavity accommodating gel piece，And this cavity is connected with air bag (3) by gas port (5).
- The negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterized in that: described cutter shaft (4) is positioned on one end of air bag (3) and is provided with positive stop (8), with limit cutter shaft (4) and molding cutter head (7) pressing after extreme position.
- The negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light the most according to claim 2, it is characterised in that: the distance of described positive stop (8) and sleeve (1) end is more than the thickness of molding cutter head (7).
- The negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterised in that: the surface configuration of described sleeve (1) is with handles (2).
- The negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterized in that: described cutter shaft (4) is provided with annular boss on one end outer wall of molding cutter head (7), described back-moving spring (6) is located on cutter shaft (4), and one end is fixed on this annular boss, the other end is fixed on the end of sleeve (1) inwall.
- The negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterised in that: described molding cutter head (7) uses with the end of sleeve (1) and threadeds, and has gap between the two, in order to change molding cutter head (7).
- The negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterised in that: described fixture (14) is movably arranged on support bar (13), to realize the regulation of sickle height.
- The negative-pressure adsorption-type gel sickle of a kind of band ultraviolet light the most according to claim 1, it is characterised in that: described transparent area (15) is strip, and is positioned on the line of molding cutter head (7) and convex lens sheet (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620410949.3U CN205616873U (en) | 2016-05-09 | 2016-05-09 | Take negative -pressure adsorption type gel exciser ware of purple light lamp |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620410949.3U CN205616873U (en) | 2016-05-09 | 2016-05-09 | Take negative -pressure adsorption type gel exciser ware of purple light lamp |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205616873U true CN205616873U (en) | 2016-10-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620410949.3U Expired - Fee Related CN205616873U (en) | 2016-05-09 | 2016-05-09 | Take negative -pressure adsorption type gel exciser ware of purple light lamp |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205616873U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106881747A (en) * | 2017-04-05 | 2017-06-23 | 巩红霞 | A kind of agar gel negative pressure forming hole method and perforating device |
| CN107322682A (en) * | 2017-08-07 | 2017-11-07 | 北京工业大学 | A kind of perforating device for micro-fluidic chip PDMS material |
-
2016
- 2016-05-09 CN CN201620410949.3U patent/CN205616873U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106881747A (en) * | 2017-04-05 | 2017-06-23 | 巩红霞 | A kind of agar gel negative pressure forming hole method and perforating device |
| CN107322682A (en) * | 2017-08-07 | 2017-11-07 | 北京工业大学 | A kind of perforating device for micro-fluidic chip PDMS material |
| CN107322682B (en) * | 2017-08-07 | 2019-07-12 | 北京工业大学 | A kind of perforating device for micro-fluidic chip PDMS material |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161005 Termination date: 20170509 |
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| CF01 | Termination of patent right due to non-payment of annual fee |