CN209793812U - disposable horizontal agarose gel electrophoresis quick gel cutter - Google Patents

Abstract

The utility model discloses a disposable horizontal agarose gel electrophoresis cuts mucilage binding fast ware. The method comprises the following steps: the rubber pressing device comprises a pressing surface (1), a first connecting handle (2), a second connecting handle (3), a handheld surface (4), a rubber pressing plate (5) and a rubber taking hole (6). The method can be used for quickly cutting a target DNA band in gel, is simple and convenient to operate and high in accuracy, and can effectively reduce the ultraviolet radiation hazard of experimental operation.

Description

Disposable horizontal agarose gel electrophoresis quick gel cutter

Technical Field

The utility model belongs to the technical field of biological assay instrument, a quick gel cutter of disposable horizontal agarose gel electrophoresis is related to.

Background

Agarose gel electrophoresis (agarose gel electrophoresis) is an electrophoresis method for separating samples of DNA, RNA, etc. using agarose as a support medium. In genetic engineering experiment operation, the target DNA band on the gel is cut off and recovered by the gel for use in related operations such as vector construction and the like. Traditional gel cutting procedures are typically performed using a scalpel blade under an ultraviolet lamp, resulting in longer exposure of the experimental procedure to ultraviolet radiation due to the need to perform a four-sided cut of the gel. Meanwhile, the manual rubber cutting has the defects of inaccurate rubber cutting surface and unstable rubber cutting amount.

Disclosure of Invention

In order to overcome the problems in the prior art, the invention provides a disposable horizontal agarose gel electrophoresis quick gel cutter which can effectively accelerate the gel cutting speed, improve the gel cutting accuracy and reduce the ultraviolet radiation hazard of experimental operation.

In order to achieve the above purpose, the utility model comprises: according to face (1), first connection handle (2), second connection handle (3), handheld face (4), rubber pressing plate (5), get gluey hole (6) and constitute, face (1) is connected with handheld face (4) by first connection handle (2) according to the pressure, face (1) is connected handle (3) by the second and is linked to each other with rubber pressing plate (5), handheld face (4) with get gluey hole (6) and link to each other, second connection handle (3) lower part is located handheld face (4) and gets gluey hole (6), rubber pressing plate (5) are located and get gluey hole (6).

The utility model discloses an useful part is: after agarose gel electrophoresis is finished, the cutting speed of a target DNA band and the accuracy of operation can be improved, and the ultraviolet radiation hazard of experimental operation is effectively reduced.

Drawings

FIG. 1 is a perspective view of a rubber cutter;

FIG. 2 is a cross-sectional view of the tape cutter (FIGS. 2a and 2b represent cross-sectional views in 2 directions);

Fig. 3 is a schematic diagram of the use of the glue cutter.

Detailed Description

The following describes the embodiments of the present invention with reference to the drawings.

as shown in fig. 1-3, the rubber cutter comprises a pressing surface (1), a first connecting handle (2), a second connecting handle (3), a handheld surface (4), a rubber pressing plate (5) and a rubber taking hole (6), wherein the pressing surface (1) is connected with the handheld surface (4) through the first connecting handle (2), the pressing surface (1) is connected with the rubber pressing plate (5) through the second connecting handle (3), the handheld surface (4) is connected with the rubber taking hole (6), the lower part of the second connecting handle (3) is positioned in the handheld surface (4) and the rubber taking hole (6), and the rubber pressing plate (5) is positioned in the rubber taking hole (6).

After completion of agarose gel electrophoresis, the gel was placed under an ultraviolet lamp. The thumb and the middle finger respectively press the two sides of the hand-held surface (4), and the index finger presses the pressing surface (1). The glue taking hole (6) is aligned with the target DNA strip and vertically cut through the gel, and the whole posture of the thumb, the middle finger and the index finger is kept unchanged in the process. And (3) turning off the ultraviolet lamp, aligning the gel taking hole (6) with the gel to a centrifugal tube for recovering the gel, keeping the posture of the thumb and the middle finger unchanged, and moving the pressing surface (1) downwards by the index finger until the gel is completely removed by the gel pressing plate (5). Since the repeated use of the gel cutter may cause DNA contamination, the gel cutter is designed to be disposable.

Claims (1)

1. The utility model provides a quick gel cutter of disposable horizontal agarose gel electrophoresis which characterized in that: the rubber cutting device is composed of a pressing face (1), a first connecting handle (2), a second connecting handle (3), a handheld face (4), a rubber pressing plate (5) and a rubber taking hole (6), wherein the pressing face (1) is connected with the handheld face (4) through the first connecting handle (2), the pressing face (1) is connected with the rubber pressing plate (5) through the second connecting handle (3), the handheld face (4) is connected with the rubber taking hole (6), the lower portion of the second connecting handle (3) is located in the handheld face (4) and the rubber taking hole (6), and the rubber pressing plate (5) is located in the rubber taking hole (6).