CN219837882U - Film cutting device for Western blot experiment - Google Patents
Film cutting device for Western blot experiment Download PDFInfo
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- CN219837882U CN219837882U CN202320599075.0U CN202320599075U CN219837882U CN 219837882 U CN219837882 U CN 219837882U CN 202320599075 U CN202320599075 U CN 202320599075U CN 219837882 U CN219837882 U CN 219837882U
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- cutting
- film
- bottom plate
- western blot
- cutting device
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- 238000005520 cutting process Methods 0.000 title claims abstract description 103
- 238000002474 experimental method Methods 0.000 title claims abstract description 27
- 238000001262 western blot Methods 0.000 title claims abstract description 27
- 108010025899 gelatin film Proteins 0.000 claims abstract description 13
- 239000012528 membrane Substances 0.000 claims description 17
- 108090000623 proteins and genes Proteins 0.000 abstract description 12
- 102000004169 proteins and genes Human genes 0.000 abstract description 12
- 238000000034 method Methods 0.000 description 10
- 238000010586 diagram Methods 0.000 description 4
- 239000002033 PVDF binder Substances 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Abstract
The utility model provides a film cutting device for a Westernblot experiment, and belongs to the technical field of medical experiments. The film cutting device for the Westernblot experiment comprises a base structure and a cutting structure, wherein the base structure comprises a cutting bottom plate, an inclined caliper, a clamp scale, a rectangular concave area and a flat bottom micro magnet. According to the utility model, the film cutting device for the Westernblot experiment is convenient for determining the position of the target protein to the greatest extent, preventing the target protein from being cut off, fixing the gel film and fine adjusting the position of the gel film, and simultaneously, cutting transversely and longitudinally, multiple purposes are achieved, a plurality of blades can be arranged on a slideway at one time, and the accuracy and efficiency are greatly improved.
Description
Technical Field
The utility model relates to the field of medical experiments, in particular to a membrane cutting device for Western blot experiments.
Background
Western blotting (Western Blot) is a commonly used experimental technique in molecular biology, and the principle is that a target protein is transferred from gel to a solid support PVDF membrane (polyvinylidene fluoride) or NC membrane (nitrocellulose) through electrophoresis separation, hybridized with a known protein antibody, and finally subjected to qualitative and quantitative analysis through a chromogenic reaction of an enzyme system. The Western Blot comprises five main steps of electrophoresis, transfer, blocking, hybridization and color development, wherein SDS-PAGE gel is required to be transferred to membrane after protein electrophoresis separation, namely, target protein is transferred to PVDF membrane or NC membrane with proper size for blocking and antibody incubation, and simultaneously, the corresponding membrane segment of the target protein is required to be cut and transferred after membrane transfer is finished.
Among the current film cutting device, the operating tool that scientific researchers adopted is mostly the simple and easy bottom plate of homemade, and scalpel and stainless steel chi are unfavorable for fixing, measuring and cutting the gel membrane, need the operator to practice repeatedly and just can master the cutting skill, and very easy misoperation leads to the experiment failure.
The Chinese patent application number is CN202021651733.9, a film cutting plate for a protein imprinting method is disclosed, the film cutting plate comprises a bottom plate and a folding transparent PE film for wrapping a film to be cut, the folding transparent PE film is of a hollow structure with four-end openings, the openings at the top and one side of the folding transparent PE film are respectively sealed by two different wide seals, and then the film to be cut can be plugged into the hollow inner side of the folding transparent PE film through the opening at the other side or the bottom of the folding transparent PE film. According to the western blotting method, the film to be cut can be prevented from being exposed to the air and dried too fast through the matched use of the folding transparent PE film, the bottom plate, the wide seal, the L-shaped pressing plate and the clamping piece, the film to be cut can be prevented from being scratched when the surface of the film to be cut is operated improperly by a user, the film to be cut is placed into the folding transparent PE film, and then is placed into the top of the bottom plate for cutting, so that damage to a tabletop or experimental objects can be effectively avoided in the cutting process.
In the scheme use process, the following defects are still present, when fixing the membrane, the pressurizing assembly is needed to be used, the operation is relatively complicated, and meanwhile, the cutting knife is not arranged, so that the cutting efficiency is low, and the practicability is reduced.
Disclosure of Invention
In order to make up for the defects, the utility model provides a film cutting device for Western blot experiments, which aims to solve the problems of low cutting efficiency and complex operation.
The embodiment of the utility model provides a film cutting device for Western blot experiments, which comprises a base structure and a cutting structure, wherein the base structure comprises a cutting bottom plate, an inclined plane caliper, a clamp type scale, a rectangular concave area and a flat bottom micro magnet, the inclined plane caliper is in sliding connection with the cutting bottom plate, the clamp type scale is in sliding connection with the cutting bottom plate, one side of the cutting bottom plate is provided with the rectangular concave area for placing a gel film, the rectangular concave area is provided with the micro magnet, the cutting structure comprises a connecting block and a cutting knife, the connecting block is in sliding connection with the cutting bottom plate, the cutting knife is in rotating connection with the connecting block, and the cutting knife is cut on the gel film.
In a specific embodiment, a first sliding block is arranged at the bottom of the bevel caliper, a first sliding groove is formed in one side of the cutting bottom plate, and the first sliding block is in sliding connection with the first sliding groove.
In the implementation process, through the sliding connection of the first sliding block and the first sliding groove, the sliding connection of the bevel caliper and the cutting bottom plate can be facilitated, and the sliding positioning is facilitated.
In a specific embodiment, a second sliding block is arranged at the bottom of the clamping type scale, a second sliding groove is formed in one side of the cutting bottom plate, and the second sliding block is in sliding connection with the second sliding groove.
In the implementation process, the second sliding block is in sliding connection with the second sliding groove, so that the sliding connection of the clamping type scale and the cutting bottom plate is facilitated, and the sliding positioning is facilitated.
In a specific embodiment, the rectangular recessed region has micromagnetism, and the flat bottom micromagnet is attracted to the rectangular recessed region.
In the implementation process, through the mutual attraction of the flat bottom micro magnet and the rectangular concave area, the gel film can be conveniently fixed, and meanwhile, the position of the gel film can be conveniently fine-tuned.
In a specific embodiment, grid lines are disposed within the rectangular recessed areas.
In the implementation process, the grid lines are arranged in the rectangular concave area, so that the parallel verification of the scale and the cutting knife can be facilitated, and the distance can be controlled.
In a specific embodiment, the two sides of the cutting bottom plate are provided with slide ways, and the connecting block is in sliding connection with the slide ways.
In the implementation process, through the connecting block and the slide sliding connection, flexible and convenient transverse and longitudinal cutting can be performed under the condition of one cutting knife, and one or more cutting knives can be arranged in the slide simultaneously to assist in cutting in different directions.
In a specific embodiment, the cutter is rotatably connected to the connection block by a pin and a nut.
In the implementation process, the cutting knife can be conveniently disassembled, assembled and replaced through the arrangement of the pin shaft and the nut.
In a specific embodiment, the cutting blade top is provided with a rubber handrail.
In the implementation process, the rubber handrail is arranged at the top of the cutting knife, so that a user can conveniently hold the blade and press the blade downwards with force.
Compared with the prior art, the utility model has the beneficial effects that: through cutting bottom plate, inclined plane slide caliper rule, card formula scale, the sunken region of rectangle and flat micro-magnet's setting, can make things convenient for the position of the furthest clear purpose albumen, prevent that the purpose albumen from being cut off, also can make things convenient for fixing the gel membrane and make things convenient for the position fine setting to the gel membrane simultaneously, through the setting of connecting block and cutting knife, can make things convenient for horizontal, fore-and-aft cutting, a sword is multi-purpose, also can set up a plurality of blades on the slide simultaneously, cuts many products of waiting to cut, has improved accuracy and efficiency greatly.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a membrane cutting device for Western blot experiments according to an embodiment of the utility model;
FIG. 2 is a schematic diagram of a side view structure of a film cutting device for Western blot experiments according to the embodiment of the utility model;
FIG. 3 is a schematic diagram of a front view structure of a film cutting device for Western blot experiments according to the embodiment of the utility model;
fig. 4 is a schematic diagram of a top view structure of a film cutting device for Western blot experiments according to an embodiment of the present utility model.
In the figure: 10-a base structure; 110-cutting the bottom plate; 120-bevel callipers; 130-card scale; 140-rectangular recessed areas; 150-a flat bottom micro magnet; 160-a first slider; 170-a second slider; 180-slideway; 20-cutting structure; 210-connecting blocks; 220-cutting knife; 230-rubber handrail.
Detailed Description
The technical solutions in the embodiments of the present utility model will be described below with reference to the accompanying drawings in the embodiments of the present utility model.
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments. All other embodiments, based on the embodiments of the utility model, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the utility model.
Examples
Referring to fig. 1, the present utility model provides a film cutting device for Western blot experiment, which comprises a base structure 10 and a cutting structure 20.
Specifically, the position of the target protein is conveniently and definitely determined to the greatest extent by the base structure 10, the target protein is prevented from being cut off, the gel film can be conveniently fixed, meanwhile, the position of the gel film is conveniently and finely adjusted, the cutting structure 20 can conveniently and transversely cut, one knife is multipurpose, a plurality of blades can be arranged on the slideway 180 at one time, a plurality of products to be cut are cut, and the accuracy and the efficiency are greatly improved.
Referring to fig. 1, 2, 3 and 4, the base structure 10 includes a cutting base 110, a bevel caliper 120, a clamp scale 130, a rectangular concave area 140 and a flat bottom micro-magnet 150, the bevel caliper 120 is slidably connected with the cutting base 110, the clamp scale 130 is slidably connected with the cutting base 110, the rectangular concave area 140 for placing a gel film is provided on one side of the cutting base 110, and the rectangular concave area 140 is provided with the micro-magnet.
When specifically setting up, inclined plane slide caliper 120 bottom is provided with first slider 160, first spout has been seted up to cutting bottom plate 110 one side, first slider 160 and first spout sliding connection, wherein, through first slider 160 and first spout sliding connection can make things convenient for inclined plane slide caliper 120 and cutting bottom plate 110's sliding connection, makes things convenient for the slip location.
When specifically setting up, card formula scale 130 bottom is provided with second slider 170, the second spout has been seted up to cutting bottom plate 110 one side, second slider 170 and second spout sliding connection, wherein, through second slider 170 and second spout sliding connection can make things convenient for card formula scale 130 and cutting bottom plate 110's sliding connection, makes things convenient for the slip location.
When specifically set up, the rectangular recessed area 140 has micromagnetism, flat bottom micro-magnet 150 with rectangular recessed area 140 attracts each other, wherein, through flat bottom micro-magnet 150 with rectangular recessed area 140 attracts each other, can conveniently fix the gel membrane and make things convenient for the position fine setting to the gel membrane simultaneously.
Referring to fig. 1, 2, 3 and 4, the cutting structure 20 includes a connection block 210 and a cutting blade 220, the connection block 210 is slidably connected with the cutting base 110, the cutting blade 220 is rotatably connected with the connection block 210, and the cutting blade 220 cuts the gel film.
In a specific setting, grid lines are disposed in the rectangular concave area 140, where the distance can be controlled by providing the grid lines in the rectangular concave area 140, so that the parallel verification of the scale and the cutter 220 can be facilitated.
When the cutting machine is specifically configured, the two sides of the cutting bottom plate 110 are provided with the slide ways 180, the connecting blocks 210 are slidably connected with the slide ways 180, wherein the flexible and convenient transverse and longitudinal cutting can be performed under the condition of one cutting knife 220 through the sliding connection of the connecting blocks 210 with the slide ways 180, and meanwhile, one or more cutting knives 220 can be arranged in the slide ways 180 to assist in cutting in different directions.
When specifically set up, the cutting knife 220 is connected with the rotation of the connecting block 210 through the pin shaft and the nut, wherein, through the setting of the pin shaft and the nut, the cutting knife 220 can be conveniently disassembled, assembled and replaced.
When the cutter blade 220 is specifically arranged, the rubber handrail 230 is arranged at the top of the cutter blade 220, wherein the rubber handrail 230 is arranged at the top of the cutter blade 220, so that a user can conveniently hold the cutter blade and press the cutter blade downwards with force.
The working principle of the film cutting device for Western blot experiments is as follows: the film cutting device for Western blot experiments is used, through the mutual attraction of the flat bottom micro magnet 150 and the rectangular concave area 140, the gel film can be conveniently fixed and simultaneously the position of the gel film can be conveniently fine-tuned, the sliding connection of the inclined plane caliper 120 and the clamp type scale 130 and the cutting bottom plate 110 is convenient for sliding positioning, the position of a target protein can be conveniently and maximally clear, the target protein is prevented from being cut off, the transverse and longitudinal cutting can be conveniently realized through the arrangement of the connecting block 210 and the cutting knife 220, the cutter is multipurpose, a plurality of blades can be also arranged on the slideway 180 at one time, and a plurality of products to be cut are cut, so that the accuracy and the efficiency are greatly improved.
It should be noted that, the specific model specification of the flat bottom micro-magnet 150 needs to be determined by selecting a model according to the actual specification of the device, and the specific model selection calculation method adopts the prior art in the field, so detailed description is omitted.
The foregoing is merely illustrative of the present utility model, and the present utility model is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present utility model. Therefore, the protection scope of the present utility model shall be subject to the protection scope of the claims.
Claims (8)
1. A cut membrane device for Western blot experiment, its characterized in that includes
The base structure (10), the base structure (10) comprises a cutting bottom plate (110), an inclined caliper (120), a clamp type scale (130), a rectangular concave area (140) and a flat bottom micro magnet (150), the inclined caliper (120) is in sliding connection with the cutting bottom plate (110), the clamp type scale (130) is in sliding connection with the cutting bottom plate (110), the rectangular concave area (140) for placing a gel film is formed in one side of the cutting bottom plate (110), and the rectangular concave area (140) is provided with the micro magnet;
the cutting structure (20), the cutting structure (20) includes connecting block (210) and cutting knife (220), connecting block (210) with cutting bottom plate (110) sliding connection, cutting knife (220) with connecting block (210) rotate to be connected, cutting knife (220) cuts in the gel membrane.
2. The film cutting device for Western blot experiments according to claim 1, wherein a first sliding block (160) is arranged at the bottom of the bevel callipers (120), a first sliding groove is formed in one side of the cutting bottom plate (110), and the first sliding block (160) is in sliding connection with the first sliding groove.
3. The film cutting device for Western blot experiments according to claim 1, wherein a second sliding block (170) is arranged at the bottom of the clamp type scale (130), a second sliding groove is formed in one side of the cutting bottom plate (110), and the second sliding block (170) is in sliding connection with the second sliding groove.
4. The film cutting device for Western blot experiments according to claim 1, wherein the rectangular recessed region (140) has micromagnetism, and the flat bottom micromagnet (150) is attracted to the rectangular recessed region (140).
5. The film cutting device for Western blot experiments according to claim 1, wherein grid lines are arranged in the rectangular recessed area (140).
6. The film cutting device for Western blot experiments according to claim 1, wherein the two sides of the cutting bottom plate (110) are provided with slide ways (180), and the connecting block (210) is in sliding connection with the slide ways (180).
7. The film cutting device for Western blot experiments according to claim 1, wherein the cutter (220) is in rotational connection with the connection block (210) through a pin and a nut.
8. The film cutting device for Western blot experiments according to claim 1, wherein a rubber handrail (230) is arranged at the top of the cutter (220).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320599075.0U CN219837882U (en) | 2023-03-24 | 2023-03-24 | Film cutting device for Western blot experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320599075.0U CN219837882U (en) | 2023-03-24 | 2023-03-24 | Film cutting device for Western blot experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219837882U true CN219837882U (en) | 2023-10-17 |
Family
ID=88303154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320599075.0U Active CN219837882U (en) | 2023-03-24 | 2023-03-24 | Film cutting device for Western blot experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219837882U (en) |
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2023
- 2023-03-24 CN CN202320599075.0U patent/CN219837882U/en active Active
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