CN201331441Y

CN201331441Y - Gelatin cutting device

Info

Publication number: CN201331441Y
Application number: CNU2008201356282U
Authority: CN
Inventors: 孙克非
Original assignee: Tiangen Biotech Beijing Co Ltd
Current assignee: Tiangen Biotech Beijing Co Ltd
Priority date: 2008-09-28
Filing date: 2008-09-28
Publication date: 2009-10-21
Anticipated expiration: 2018-09-28

Abstract

The utility model belongs to technologies used in the nucleic acid extraction field, and particularly relates to a gelatin cutting device. The device is a hollow plastic device which consists of a swollen closed end at the upper part, a portion with two recessed sides in the middle, and a narrow opening end at the lower part; the swollen closed end at the upper part is pressed to transfer a cut gelatin block to a container for future use; the recessed portion in the middle, which serves as a cushion, can stop the cut gelatin block from being directly absorbed into the swollen portion at the upper part; and the aperture width of the lower part is determined based on the size of an ordinary electrophoretic band. The gelatin cutting device has the characteristics of simple and rapid operation as well as low cost, produces no pollution, effectively guarantees the safety of operators during operation, and avoids the pollution and the potential harm to the operators during the use of blades or scissors for cutting gelatin bands.

Description

The gel cutter sweep

Technical field

The invention belongs to the technology in nucleic acid extraction field, relate in particular to a kind of gel cutting technique.

Background technology

Gel electrophoresis is widely used in separating in the biological technical field biomolecule of target biology, as DNA isolation from biomolecules mixture, RNA or albumen.In the scientific research field or production and quality testing field of separation of biomolecules from gel, wish from glue, to obtain target biomolecule usually and be used for subsequent analysis.And this invention is and a kind ofly can shifts target biomolecule from glue and transfer in the container, as the device in the Eppendorf pipe.

The operation of excision purpose band can be subjected to using scissors or blade operation in the excision process and use tweezers or the influence of the operation when similarly instrument is transferred to receiving vessel from running gel.And one of important target of biotechnology is exactly the high performance reproducibility that guarantees experimental result.Yet the changeability of glue technology is cut in craft, and this just makes that the repeatability that obtains the target biomolecule result has constantly caused influence.

The consistance that lacks the gel cutting can cause the variation of DNA purifying output.In the gel electrophoresis process, big molecular migration forms band, and the migration distance during each band electrophoresis is decided by biomolecule size, type, purity and the electric charge of band.If the target biomolecule migration has formed compact band, help from other molecule, separating better, operation has repeatable preferably.Cut glue if desired, but electrophoretic band is loose can not separate from other biomolecule preferably the time, cutting operation just is difficult to guarantee the unicity of the band of repeated sample.Result's changeability can show in various degree biologically active by biological specimen that contains or different isomerss, but the difference of stripe size also can cause the marked change of ensuing activity analysis.

There is safety issue in the operating process in the common gluing method of cutting, the one operator might be switched to by blade in operating process, and when cutting gel band, if adopt radiolabeled biomolecule also can cause health threat during electrophoresis, in the process of cutting band, perhaps potential hazardous radiation material can be touched on the skin the operator.It two is that the common gluing method of cutting also needs the band that cuts down with utensils such as cutter or scissors is transferred in another container, and cutter or scissors all are recycling usually, so in the transfer process of band, also can cause the cross pollution of bar interband perhaps, influence the repeatability of experimental result.

In sum owing to manual cut many shortcomings that the glue technology exists, be necessary to develop a kind of can cut and the transfer adhesive band cut adhesive dispenser, this device has following advantage: 1) cut and shift big minute subsample at an easy rate, keep the pure of sample; 2) keep maximum repeatability in the band cutting operation process; 3) the protection operator avoids health hazard.

External existing several adhesive dispensers of cutting comprise U.S.Pat.No.6,565,728, U.S.Pat.No.6,393,926, U.S.Pat.No.6,342,143 and U.S.Pat.No.5,587,062 etc., these devices respectively have superiority, but all design is more loaded down with trivial details, complicated operation, for only isolating purpose adhesive tape band and reclaim slightly complicated the experiment from gel, based on existing problem of cutting adhesive dispenser, we have invented this simple and convenient adhesive dispenser of cutting fast.

Present invention is a kind of adhesive dispenser of cutting, and this device can simply fly-cutting adhesive tape band and transfer adhesive band, can not cause the pollution in the transfer process, is applicable to from cutting out pure band the running gel widely.

Summary of the invention

Primary and foremost purpose of the present invention provides a kind of reliable gel cutter sweep, can repeatedly cut pure blob of viscose from gel, is applied to follow-up biomolecule analysis operation.

Another object of the present invention provides cutting glue and shifting the device of blob of viscose of a kind of quick and easy one-handed performance.

A kind of gel cutter sweep of the present invention is characterized in that described gel cutter sweep expands blind end by a top, the hollow plastic device that the thin narrow opening end in middle both sides depressed part and bottom is formed.

This device is simple to operate, with low cost, the size in hole is according to common electrophoretic band size design, and be disposable product, avoided adopting blade or scissors to cut inaccurate and the cross pollution between the band that causes of cutting in the glue process in the past, make also that simultaneously the stripe size of at every turn cutting glue is consistent, strengthened the comparability of repetitive operation test.The operator can wear gloves and pinch and cut Jiao Kou top and expand the position, reduced the danger that adopts blade or scissors to cut contact glue in the glue process, and this device makes can not cause for plastics and adopts that mistake switches to operator's danger in blade or the scissors cut blob of viscose process, thereby improved the security of cutting the glue operation.

In sum, this device has advantage simple to operate, with low cost, safe in utilization, has good practical value and application prospect aspect the gel recycling in the nucleic acid extraction field.

Description of drawings

Fig. 1. gel is cut adhesive dispenser figure, and 1 expands blind end for top, and 2 is middle both sides depressed parts, and 3 is the thin narrow opening end in bottom, and three parts are in conjunction with as a whole hollow devices.

Fig. 2. cut glue operating process synoptic diagram, hold top and expand blind end, will cut the thin narrow opening end in adhesive dispenser bottom and aim at the purpose band and vertically insert in the glue.

Fig. 3. front and back firmly are cut up to the purpose band back and forth.

Fig. 4. from glue, extract cutter sweep out.

Fig. 5. extruding top is expanded blind end the purpose band is transferred in the Eppendorf pipe.

Embodiment

Below in conjunction with accompanying drawing the utility model is further described.

Embodiment 1

According to Fig. 1, this routine gel is cut adhesive dispenser for expanded blind end by a top, and the hollow plastic device that the thin narrow opening end in middle both sides depressed part and bottom is formed is cut the glue experiment with this device, and concrete operation method is as follows:

1) according to Fig. 2, hold top and expand blind end (please don't push), will cut the thin narrow opening end in adhesive dispenser bottom and aim at the purpose band and vertically insert in the glue.

2) according to Fig. 3, exert oneself back and forth in front and back, up to the purpose band being cut down (please don't push top expand blind end).

3), from glue, extract cutter sweep (please don't push top expand blind end) out according to Fig. 4.

4) according to Fig. 5, will cut the thin narrow opening end in adhesive dispenser bottom and aim at the Eppendorf pipe, firmly push top and expand blind end, blob of viscose is transferred in the pipe.

Claims

1. gel cutter sweep is characterized in that described gel cutter sweep expands blind end by a top, the hollow plastic device that the thin narrow opening end in middle both sides depressed part and bottom is formed.