CN103522324A - Gel special cutter - Google Patents
Gel special cutter Download PDFInfo
- Publication number
- CN103522324A CN103522324A CN201310494375.3A CN201310494375A CN103522324A CN 103522324 A CN103522324 A CN 103522324A CN 201310494375 A CN201310494375 A CN 201310494375A CN 103522324 A CN103522324 A CN 103522324A
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- China
- Prior art keywords
- gel
- push plate
- push pedal
- lower push
- connecting rod
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Abstract
The invention discloses a gel special cutter. According to the technical scheme of the gel special cutter, an upper push plate is connected with a connecting rod and a lower push plate in sequence, crochet hook holes are formed in the lower push plate, and crochet hooks are arranged in the crochet hook holes. The upper push plate, the connecting rod and the lower push plate are integrally arranged in a box body. The periphery of the lower end of the box body is provided with cutting blades. The upper push plate, the connecting rod, the lower push plate and the box body are all made of transparent materials. Through the gel special cutter, a target strip can be accurately selected and then be accurately cut off. Meanwhile, a gel block cut off can be observed at multiple angles so that redundant gel can be cut off and the recovery rate of DNA can be improved. Through the crochet hooks arranged on the lower push plate, the problem that the cutter can not be inserted into the polyacrylamide gel due to thin, soft and smooth colloid of the polyacrylamide gel is solved, the gel block cut off can be directly taken away from a cutting table, the time of the DNA exposed to ultraviolet light is greatly shortened, and the degradation rate of the DNA is effectively reduced. The gel special cutter is simple in structure, easy to operate and special in effect.
Description
Technical field
The present invention relates to a kind of Special cutting knife, specifically a kind of for cutting the Special cutting knife of Ago-Gel or polyacrylamide gel, belong to special tool technical field.
Background technology
In bioscience, gel electrophoresis technology is realized the separated of the materials such as protein, nucleic acid and amino acid and identifies according to the difference of the electric charge of charging cpd, molecular size and shape.Ago-Gel and polyacrylamide gel are the electrophoresis solid supports being most widely used at present.For realizing the separation to target material, particularly, when reclaiming target DNA band, need to cut gel, and reclaim target dna fragment.For improving the rate of recovery of DNA, while cutting glue, to try one's best unnecessary gel is cut away, and cut glue action and want fast, if ultraviolet irradiation time is long, will cause DNA Partial digestion.
The most frequently used gel cutting cutter is blade or the scalpel that a kind of common metal is made at present, and operating personnel hold blade or scalpel handle of a knife all around cuts at four sides along object fragment.This cutter are wasted time and energy, and when cutting be not easy to grasp direction, easily there is the phenomenon of cutting more or cutting less, thus the rate of recovery of reduction DNA.State Intellectual Property Office discloses a kind of Ago-Gel cutter (application number: 201320044963) in 2013, this cutter comprises housing and handle, housing is the cube cavity that there is no upper base and go to the bottom, the side of cube cavity is surrounded by four rectangular blades, and handle is fixedly connected with the top of described blade.Operating personnel hold handle, and the gel that housing is aimed at need to cut is also cut down, by upper base or go to the bottom gel is pushed.Because this cutter blade is that metal material is light tight, can only observe from top to bottom to confine target dna band, whether have not containing the gel of DNA, limited the further finishing to blob of viscose if after having cut, cannot observe from the side blob of viscose top and the bottom.In addition concerning conventionally very thin polyacrylamide gel, though these cutter can cut target stripe, but because film matter is thin, soft, smooth, can not be embedded in and in cutter, take away cutting bed, need with aid, the band cutting be chosen from cutting bed again, as cut a plurality of bands on a swimming lane, this will increase the irradiation time of ultraviolet ray to DNA, is easy to occur chaotic.
Summary of the invention
For overcoming the existing defect of existing gel cutting cutter, realize accurate, fly-cutting to Ago-Gel and polyacrylamide gel, the invention discloses a kind of gel Special cutting knife.
The present invention is achieved by the following technical solutions:
Upper push pedal connects connecting rod and lower push pedal successively, in lower push pedal, have crochet hook hole, crochet hook is equipped with crochet hook in hole, and upper push pedal, connecting rod and lower push pedal integral body are placed in casing, under casing, end periphery is equipped with cutting blade, and upper push pedal, connecting rod, lower push pedal, casing all adopt transparent material to make.
The present invention is owing to all adopting transparent material to make, so accurate select target band and accurately cuts it, the blob of viscose simultaneously also can multi-angle observation cutting, to cut away unnecessary gel, improves the rate of recovery of DNA.By the crochet hook being arranged in lower push pedal, solved because of thin, soft, the smooth problem that can not embed cutter of polyacrylamide gel matter simultaneously, can directly the blob of viscose cutting be taken away to cutting bed, greatly reduce DNA and be exposed to the ultraviolet time, effectively reduced the degradation rate of DNA.The present invention is simple in structure, easily operation, and effect is peculiar.
Accompanying drawing explanation
Accompanying drawing 1 is main TV structure generalized section of the present invention.
Accompanying drawing 2 is upward views of accompanying drawing 1 of the present invention.
Description of reference numerals is as follows: upper push pedal 1, connecting rod 2, lower push pedal 3, casing 4, cutting blade 5, crochet hook hole 6, crochet hook 7.
The specific embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail:
Utilize the present invention when cutting polyacrylamide gel, hold casing upper end, on gel imaging instrument, make cutting blade aim at the gel that will cut and firmly gel be cut downwards, then gently press in the gel piece that push pedal thrusts crochet hook to cut, hold casing cutter are extracted, put into centrifuge tube, touch push pedal gel piece is released, on centrifugal tube wall, flick, gel piece is fallen in centrifuge tube.
Utilize the present invention when cutting Ago-Gel, crochet hook can be taken off, hold casing upper end, on gel imaging instrument, make cutting blade aim at the gel that will cut and firmly gel be cut downwards, hold casing cutter are extracted, the gel piece from multi-angle observation casing, is confirmed whether to repair the gel piece cutting, if need to, touch push pedal gel piece is released, cut away unnecessary gel; If do not needed, directly gel piece is pushed in centrifuge tube.
Claims (2)
1. a gel Special cutting knife, it is characterized in that being achieved through the following technical solutions: upper push pedal (1) connects connecting rod (2) and lower push pedal (3) successively, in lower push pedal (3), have crochet hook hole (6), crochet hook (7) is housed in crochet hook hole (6), upper push pedal (1), connecting rod (2) are placed in casing (4) with lower push pedal (3) integral body, the lower end periphery of casing (4) is equipped with cutting blade (5), and upper push pedal (1), connecting rod (2), lower push pedal (3), casing (4) all adopt transparent material to make.
2. a kind of gel Special cutting knife according to claim 1, is characterized in that connecting rod (2) and the total height of lower push pedal (3) are equal to or greater than the total height of casing (4) and cutting blade (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310494375.3A CN103522324A (en) | 2013-10-21 | 2013-10-21 | Gel special cutter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310494375.3A CN103522324A (en) | 2013-10-21 | 2013-10-21 | Gel special cutter |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103522324A true CN103522324A (en) | 2014-01-22 |
Family
ID=49924851
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310494375.3A Pending CN103522324A (en) | 2013-10-21 | 2013-10-21 | Gel special cutter |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103522324A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104960006A (en) * | 2015-06-04 | 2015-10-07 | 上海海洋大学 | Automatic anti-toxin gel cutting machine, imager and gel cutting method of anti-toxin automatic gel cutting machine |
| CN106078811A (en) * | 2016-08-24 | 2016-11-09 | 西南医科大学 | A kind of adjustable gel cutting device |
| CN106313175A (en) * | 2016-09-09 | 2017-01-11 | 天津建昌环保股份有限公司 | Hot melting perforation device for perforating square hole in PE pipe |
| CN108858293A (en) * | 2018-05-24 | 2018-11-23 | 福建农林大学 | Visual row's formula running gel rubber knife and its application method |
| CN114986610A (en) * | 2022-06-17 | 2022-09-02 | 中国农业大学 | Portable matrix bag perforating device |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4843716A (en) * | 1980-11-24 | 1989-07-04 | L.K. Manufacturing Corp. | Canape maker |
| WO2001094526A2 (en) * | 2000-06-08 | 2001-12-13 | Elchrom Scientific Ag | A gel cutting and recovering device |
| US6393926B1 (en) * | 2000-05-19 | 2002-05-28 | Accutrol Co., Inc. | Front-loading precision material sampler with interchangeable retracting chamber |
| CN2589981Y (en) * | 2002-09-16 | 2003-12-03 | 中国农业科学院哈尔滨兽医研究所 | Gelose punch |
| US20060102481A1 (en) * | 2004-11-16 | 2006-05-18 | Chen Stephen L | Open frame gel casting in submarine gel electrophoresis |
| CN201331441Y (en) * | 2008-09-28 | 2009-10-21 | 天根生化科技（北京）有限公司 | Gelatin cutting device |
| CN202517508U (en) * | 2012-04-13 | 2012-11-07 | 中国农业科学院兰州畜牧与兽药研究所 | Spot clipped machine of gel |
| CN203003933U (en) * | 2013-01-21 | 2013-06-19 | 齐齐哈尔医学院 | Electrophoresis gel cutting knife |
| CN203045770U (en) * | 2013-01-28 | 2013-07-10 | 邯郸市康业生物科技有限公司 | Syringe type gel cutter |
-
2013
- 2013-10-21 CN CN201310494375.3A patent/CN103522324A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4843716A (en) * | 1980-11-24 | 1989-07-04 | L.K. Manufacturing Corp. | Canape maker |
| US6393926B1 (en) * | 2000-05-19 | 2002-05-28 | Accutrol Co., Inc. | Front-loading precision material sampler with interchangeable retracting chamber |
| WO2001094526A2 (en) * | 2000-06-08 | 2001-12-13 | Elchrom Scientific Ag | A gel cutting and recovering device |
| US6565728B1 (en) * | 2000-06-08 | 2003-05-20 | Elchrom Scientific | Gel cutting and recovering device |
| CN2589981Y (en) * | 2002-09-16 | 2003-12-03 | 中国农业科学院哈尔滨兽医研究所 | Gelose punch |
| US20060102481A1 (en) * | 2004-11-16 | 2006-05-18 | Chen Stephen L | Open frame gel casting in submarine gel electrophoresis |
| CN201331441Y (en) * | 2008-09-28 | 2009-10-21 | 天根生化科技（北京）有限公司 | Gelatin cutting device |
| CN202517508U (en) * | 2012-04-13 | 2012-11-07 | 中国农业科学院兰州畜牧与兽药研究所 | Spot clipped machine of gel |
| CN203003933U (en) * | 2013-01-21 | 2013-06-19 | 齐齐哈尔医学院 | Electrophoresis gel cutting knife |
| CN203045770U (en) * | 2013-01-28 | 2013-07-10 | 邯郸市康业生物科技有限公司 | Syringe type gel cutter |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104960006A (en) * | 2015-06-04 | 2015-10-07 | 上海海洋大学 | Automatic anti-toxin gel cutting machine, imager and gel cutting method of anti-toxin automatic gel cutting machine |
| CN106078811A (en) * | 2016-08-24 | 2016-11-09 | 西南医科大学 | A kind of adjustable gel cutting device |
| CN106313175A (en) * | 2016-09-09 | 2017-01-11 | 天津建昌环保股份有限公司 | Hot melting perforation device for perforating square hole in PE pipe |
| CN106313175B (en) * | 2016-09-09 | 2019-01-29 | 天津建昌环保股份有限公司 | A kind of PE pipe opens the hot melt boring device of square hole |
| CN108858293A (en) * | 2018-05-24 | 2018-11-23 | 福建农林大学 | Visual row's formula running gel rubber knife and its application method |
| CN114986610A (en) * | 2022-06-17 | 2022-09-02 | 中国农业大学 | Portable matrix bag perforating device |
| CN114986610B (en) * | 2022-06-17 | 2022-11-18 | 中国农业大学 | Portable matrix bag perforating device |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140122 |