CN214025854U - Gel excision device for DNA synthesis - Google Patents
Gel excision device for DNA synthesis Download PDFInfo
- Publication number
- CN214025854U CN214025854U CN202022746017.5U CN202022746017U CN214025854U CN 214025854 U CN214025854 U CN 214025854U CN 202022746017 U CN202022746017 U CN 202022746017U CN 214025854 U CN214025854 U CN 214025854U
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- Prior art keywords
- fixedly connected
- motor
- plate
- gel
- dna synthesis
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- 230000006820 DNA synthesis Effects 0.000 title claims description 24
- 239000006096 absorbing agent Substances 0.000 claims 1
- 238000005520 cutting process Methods 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 3
- 238000002271 resection Methods 0.000 abstract description 3
- 239000012634 fragment Substances 0.000 description 8
- UQMRAFJOBWOFNS-UHFFFAOYSA-N butyl 2-(2,4-dichlorophenoxy)acetate Chemical compound CCCCOC(=O)COC1=CC=C(Cl)C=C1Cl UQMRAFJOBWOFNS-UHFFFAOYSA-N 0.000 description 5
- 238000010586 diagram Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a gel resection device for DNA is synthetic, including base, first motor, second motor, first electric putter and second electric putter, two connecting plates of last fixed surface of base, the equal joint of opposite face of two connecting plates have first bearing, and the internal surface of two first bearings all is provided with first pivot, two relative one ends of first pivot respectively with the both ends fixed connection of first screw post, the surperficial threaded connection of first screw post has first screw cap. The utility model discloses owing to set up and place board, cutting knife, first motor, second motor, first electric putter, conical shell, laser lamp, three-way pipe and second electric putter, make synthetic gel resection device of DNA can be more accurate advance the excision to synthetic gel of DNA, owing to do not need people directly to operate through the hand at the in-process of excision, avoided receiving the pollution at the synthetic gel of in-process DNA of excision.
Description
Technical Field
The utility model relates to a DNA synthesis's gel technical field specifically is a gel resection device for DNA synthesis.
Background
The gel recovery of DNA fragments is a basic operation of molecular biology experiments, which is to cut and recover the gel at the position of the whole target fragment, and the most common gel cutting method at present is to cut the target band as thin as possible by using a scalpel, but the efficiency is very low, and the NDA synthetic gel fragments needing to be cut cannot be accurately cut by manual operation, and most of the DNA synthetic gel may be polluted by manual operation in the cutting process.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a gel excision device for DNA synthesis to solve the problems in the background technology.
In order to achieve the above object, the utility model provides a following technical scheme:
a gel cutting device for DNA synthesis comprises a base, a first motor, a second motor, a first electric push rod and a second electric push rod, wherein the upper surface of the base is fixedly connected with two connecting plates, the opposite surfaces of the two connecting plates are respectively clamped with a first bearing, the inner surfaces of the two first bearings are respectively provided with a first rotating shaft, the opposite ends of the two first rotating shafts are respectively fixedly connected with the two ends of a first threaded column, the surface of the first threaded column is in threaded connection with a first threaded cap, the first threaded cap is clamped on the front surface of a first movable plate, the upper surface of the first movable plate is fixedly connected with the lower surface of a placing plate, and the front end of the first rotating shaft positioned on the front side is fixedly connected with an output shaft of the first motor; the upper surface of the base is fixedly connected with two fixed plates, the opposite surfaces of the two fixed plates are respectively clamped with a second bearing, the inner surfaces of the two second bearings are respectively sleeved with a second rotating shaft, the opposite ends of the two second rotating shafts are respectively fixedly connected with the left end and the right end of a second threaded column, the right end of the second rotating shaft positioned on the right side is fixedly connected with an output shaft of a second motor, the surface of the second threaded column is in threaded connection with a second threaded cap, the second threaded cap is clamped on the right side surface of a second movable plate, and the lower surface of the second movable plate is fixedly connected with a first transverse plate;
the lower surface of the first transverse plate is fixedly connected with the upper surface of the second transverse plate through a first electric push rod, the upper surface of the second transverse plate is fixedly connected with a third slide bar, the surface of the third slide bar is sleeved with a third slide sleeve, the third slide sleeve is clamped on the upper surface of the first transverse plate, the top end of the third slide bar is fixedly connected with the lower surface of the limiting block, the lower surface of the second transverse plate is clamped with a conical shell, a piston is arranged in the conical shell, the upper surface of the piston is fixedly connected with a fourth slide bar, the surface of the third slide bar is sleeved with a fourth slide sleeve, the fourth slide sleeve is clamped on the upper surface of the conical shell, the top end of the third slide bar is fixedly connected with the lower surface of the supporting plate, and the lower surface of the supporting plate is fixedly connected with the upper surface of the conical shell through a second electric push rod; the lower surface of the conical shell is provided with a drainage pipe, the bottom end of the drainage pipe is provided with a three-way pipe, the three-way pipe is provided with a first one-way valve and a second one-way valve, one end of the three-way pipe is communicated with a through hole, the through hole is formed in the upper surface of the fixing block, and the upper surface of the fixing block is provided with a connecting shell;
the inner surface of the connecting shell is provided with internal threads, the surface of one end of the three-way pipe is provided with external threads, the internal threads are connected with the external threads, and the front surface, the back surface, the left side surface and the right side surface of the fixing block are provided with laser lamps; the lower surface fixed connection of fixed plate has four blades, four blades enclose city rectangle.
As a further aspect of the present invention: the front of first fly leaf joint has first sliding sleeve, and the internal surface of first sliding sleeve is provided with first slide bar, the both ends of first slide bar respectively with the opposite face fixed connection of two connecting plates.
As a further aspect of the present invention: the right side face of the second movable plate is connected with a second sliding sleeve in a clamping mode, a second sliding rod is sleeved on the inner surface of the second sliding sleeve, and two ends of the second sliding rod are fixedly connected with the opposite faces of the two fixing plates respectively.
As a further aspect of the present invention: the lower fixed surface of first motor fuselage is connected with the frame, and the lower surface of frame and the upper surface fixed connection of base.
As a further aspect of the present invention: the upper surface and the lower surface of the second motor body are respectively fixedly connected with opposite surfaces of the two fixing rods, and the left ends of the two second fixing rods are fixedly connected with the right side surface of the right side fixing plate.
Compared with the prior art, the utility model has the advantages that the placing plate, the cutting knife, the first motor, the second motor, the first electric push rod, the conical shell, the laser lamp, the three-way pipe and the second electric push rod are arranged, so that the laser lamp can position the cutting position, the first motor and the second motor can respectively drive the placing plate and the blades to move, the DNA synthetic gel to be cut is positioned between the four blades, the first electric push rod can drive the blades to move downwards to cut the DNA synthetic gel, the second electric push rod can drive the piston to move upwards to suck the cut DNA synthetic gel into the conical shell, the DNA synthetic gel can be discharged through the other end of the three-way pipe, the taking out and the collection are convenient, therefore, people do not need to manually cut the DNA synthetic gel, the DNA synthetic gel cutting device can cut the DNA synthetic gel more accurately, and because people do not need to directly put through hands to operate in the cutting process, contamination of the DNA synthesis gel during excision is avoided.
Drawings
FIG. 1 is a schematic sectional view of a gel excision apparatus for DNA synthesis.
FIG. 2 is a schematic diagram of the structure of the first movable plate viewed from the right in a gel excision apparatus for DNA synthesis.
FIG. 3 is a schematic diagram showing an enlarged structure of the A site in a gel excision apparatus for DNA synthesis.
In the figure: 1. a base; 2. a connecting plate; 3. a machine base; 4. a first motor; 5. a first rotating shaft; 6. a first threaded post; 7. a first threaded cap; 8. a first movable plate; 9. a first slide bar; 10. a first sliding sleeve; 11. placing the plate; 12. a fixing plate; 13. fixing the rod; 14. a second motor; 15. a second rotating shaft; 16. a second threaded post; 17. a second threaded cap; 18. a second movable plate; 19. a second sliding sleeve; 20. a second slide bar; 21. a first transverse plate; 22. a first electric push rod; 23. a second transverse plate; 24. a third sliding sleeve; 25. a third slide bar; 26. a conical shell; 27. a piston; 28. a fourth slide bar; 29. a fourth sliding sleeve; 30. drawing and releasing the pipe; 31. a three-way pipe; 32. a first check valve; 33. a second one-way valve; 34. a through hole; 35. a fixed block; 36. a laser light; 37. a blade; 38. a support plate; 39. a second electric push rod; 40. the connecting shell.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Referring to fig. 1-3, a gel cutting device for DNA synthesis includes a base 1, a first motor 4, a second motor 14, a first electric push rod 22 and a second electric push rod 39, wherein the upper surface of the base 1 is fixedly connected with two connecting plates 2, first bearings are respectively clamped on opposite surfaces of the two connecting plates 2, first rotating shafts 5 are respectively arranged on inner surfaces of the two first bearings, opposite ends of the two first rotating shafts 5 are respectively fixedly connected with two ends of a first threaded column 6, a first screw cap 7 is connected on the surface of the first threaded column 6 through screw threads, and the first screw cap 7 is clamped on the front surface of a first movable plate 8.
The front surface of the first movable plate 8 is clamped with a first sliding sleeve 10, the inner surface of the first sliding sleeve 10 is provided with a first sliding rod 9, due to the arrangement of the first sliding rod 9 and the first sliding sleeve 10, the first sliding sleeve 10 is more stable when moving left and right on the surface of the first sliding rod 9, so that the first movable plate 8 is more stable when moving left and right through the first sliding sleeve 10, two ends of the first sliding rod 9 are respectively and fixedly connected with the opposite surfaces of the two connecting plates 2, the upper surface of the first movable plate 8 is fixedly connected with the lower surface of the placing plate 11, and the front end of the first rotating shaft 5 positioned at the front side is fixedly connected with the output shaft of the first motor 4; the lower fixed surface of the first motor 4 fuselage is connected with frame 3, and the lower surface of frame 3 is connected with the upper surface fixed of base 1.
The upper surface of the base 1 is fixedly connected with two fixed plates 12, the opposite surfaces of the two fixed plates 12 are respectively clamped with a second bearing, the inner surfaces of the two second bearings are respectively sleeved with a second rotating shaft 15, the opposite ends of the two second rotating shafts 15 are respectively fixedly connected with the left end and the right end of a second threaded column 16, and the right end of the second rotating shaft 15 positioned on the right side is fixedly connected with an output shaft of a second motor 14; the upper surface and the lower surface of the body of the second motor 14 are respectively fixedly connected with the opposite surfaces of the two fixing rods 13, the left ends of the two second fixing rods 13 are fixedly connected with the right side surface of the right fixing plate 12, the surface thread of the second threaded column 16 is connected with a second threaded cap 17, and the second threaded cap 17 is connected to the right side surface of the second movable plate 18 in a clamping manner.
The right side surface of the second movable plate 18 is clamped with a second sliding sleeve 19, the inner surface of the second sliding sleeve 19 is sleeved with a second sliding rod 20, two ends of the second sliding rod 20 are respectively fixedly connected with the opposite surfaces of the two fixed plates 12, and the lower surface of the second movable plate 18 is fixedly connected with a first transverse plate 21.
The lower surface of the first transverse plate 21 is fixedly connected with the upper surface of the second transverse plate 23 through the first electric push rod 22, the upper surface of the second transverse plate 23 is fixedly connected with a third slide rod 25, a third slide sleeve 24 is sleeved on the surface of the third slide rod 25, the third slide sleeve 24 is connected to the upper surface of the first transverse plate 21 in a clamped mode, the top end of the third slide rod 25 is fixedly connected with the lower surface of the limiting block, a conical shell 26 is connected to the lower surface of the second transverse plate 23 in a clamped mode, a piston 27 is arranged in the conical shell 26, a fourth slide rod 28 is fixedly connected to the upper surface of the piston 27, a fourth slide sleeve 29 is sleeved on the surface of the third slide rod 25, the fourth slide sleeve 29 is connected to the upper surface of the conical shell 26 in a clamped mode, the top end of the third slide rod 25 is fixedly connected with the lower surface of the supporting plate 38, and the lower surface of the supporting plate 38 is fixedly connected with the upper surface of the conical shell 26 through the second electric push rod 39.
The lower surface of the conical shell 26 is provided with a drainage pipe 30, the bottom end of the drainage pipe 30 is provided with a three-way pipe 31, the three-way pipe 31 is provided with a first one-way valve 32 and a second one-way valve 33, the three-way pipe 31, the drainage pipe 30, the first one-way valve 32 and the second one-way valve 33 are arranged, so that the cut DNA synthetic gel fragments can be conveniently sucked into the conical shell 26 and discharged from the conical shell 26, the cut DNA synthetic gel fragments can be conveniently collected by a worker, one end of the three-way pipe 31 is communicated with a through hole 34, the through hole 34 is arranged on the upper surface of a fixing block 35, and the upper surface of the fixing block 35 is provided with a connecting shell 40; the inner surface of the connecting shell 40 is provided with an inner thread, the surface of one end of the three-way pipe 31 is provided with an outer thread, the inner thread is connected with the outer thread, due to the arrangement of the inner thread, the outer thread and the connecting shell 40, when the size of DNA gel fragments needing to be cut changes, the DNA synthetic gel fragments can be better cut by the blades 37 with different sizes, the front, the back, the left side and the right side of the fixing block 35 are provided with the laser lamps 36, and due to the arrangement of the laser lamps 36, the light irradiated by the laser lamps 36 can position the DNA synthetic gel fragments needing to be cut; the lower surface of the fixing plate 12 is fixedly connected with four blades 37, and the four blades 37 enclose a city rectangle.
The utility model discloses a theory of operation is: when the DNA synthesis gel needs to be cut, the DNA synthesis gel needing to be cut is placed on the placing plate 11, the four laser lamps 36 are turned on, the first motor 4 is started again, the first motor 4 is made to drive the first rotating shaft 5 located on the right side to rotate, the first rotating shaft 5 located on the right side is made to drive the first threaded column 6 to rotate, the first threaded column 6 and the first threaded cap 7 are made to generate threaded motion, the first threaded cap 7 is made to drive the placing plate 11 to move through the first movable plate 8, and the placing plate 11 is made to drive the DNA synthesis gel to move to the central position, where the laser light 36 located on the front side and the rear side of the fixing block 35 irradiates laser.
Then the second motor 14 is started, the second motor 14 drives the second rotating shaft 15 on the right side to rotate, the second rotating shaft 15 drives the second threaded column 16 to rotate, the second threaded column 16 and the second threaded cap 17 generate threaded motion, the second threaded cap 17 drives the second movable plate 18 to move, so that the blades 37 move, the four blades 37 move to the position above the position where the DNA synthesis gel needs to be cut, the first electric push rod 22 is started, the first electric push rod 22 drives the second transverse plate 23 to move downwards, the four blades 37 move downwards, the DNA synthesis gel needing to be cut is cut, the second one-way valve 33 is opened, the second electric push rod 39 is started, the second electric push rod 39 drives the support plate 38 to move upwards, the second support plate 38 drives the fourth slide rod 28 to move upwards, and the fourth slide rod 28 drives the piston 27 to move upwards, the cut DNA synthesis gel is sucked into the conical shell 26, when the DNA synthesis gel needs to be taken out, the first valve is opened, the second electric push rod 39 is started, the second electric push rod 39 drives the piston 27 to move downwards through the support plate 38 and the fourth slide bar 28, and the DNA synthesis gel in the conical shell 26 is discharged through the other end of the three-way pipe 31.
When the blade 37 needs to be replaced, the fixing block 35 is screwed, the fixing block 35 drives the connecting shell 40 to rotate, the connecting shell 40 drives the internal thread to rotate, the internal thread and the external thread generate thread movement, and the replaced blade 37 is installed in the same way.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
Although the preferred embodiments of the present patent have been described in detail, the present patent is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present patent within the knowledge of those skilled in the art.
Claims (5)
1. A gel excision device for DNA synthesis comprises a base (1), a first motor (4), a second motor (14), a first electric push rod (22) and a second electric push rod (39), the movable type electric shock absorber is characterized in that the upper surface of the base (1) is fixedly connected with two connecting plates (2), first bearings are clamped on opposite surfaces of the two connecting plates (2), first rotating shafts (5) are arranged on inner surfaces of the two first bearings, opposite ends of the two first rotating shafts (5) are fixedly connected with two ends of a first threaded column (6) respectively, surface threads of the first threaded column (6) are connected with first threaded caps (7), the first threaded caps (7) are clamped on the front surfaces of first movable plates (8), the upper surfaces of the first movable plates (8) are fixedly connected with the lower surface of a placing plate (11), and the front ends of the first rotating shafts (5) on the front side are fixedly connected with output shafts of a first motor (4); the upper surface of the base (1) is fixedly connected with two fixed plates (12), the opposite surfaces of the two fixed plates (12) are respectively clamped with second bearings, the inner surfaces of the two second bearings are respectively sleeved with a second rotating shaft (15), the opposite ends of the two second rotating shafts (15) are respectively fixedly connected with the left end and the right end of a second threaded column (16), the right end of the second rotating shaft (15) positioned on the right side is fixedly connected with an output shaft of a second motor (14), the surface threads of the second threaded column (16) are connected with a second threaded cap (17), the second threaded cap (17) is clamped on the right side surface of a second movable plate (18), and the lower surface of the second movable plate (18) is fixedly connected with a first transverse plate (21);
the lower surface of the first transverse plate (21) is fixedly connected with the upper surface of the second transverse plate (23) through a first electric push rod (22), the upper surface of the second transverse plate (23) is fixedly connected with a third slide bar (25), the surface of the third slide bar (25) is sleeved with a third slide sleeve (24), the third slide sleeve (24) is connected with the upper surface of the first transverse plate (21) in a clamping manner, the top end of the third slide bar (25) is fixedly connected with the lower surface of a limiting block, the lower surface of the second transverse plate (23) is connected with a conical shell (26), a piston (27) is arranged in the conical shell (26), the upper surface of the piston (27) is fixedly connected with a fourth slide bar (28), the surface of the third slide bar (25) is sleeved with a fourth slide sleeve (29), the fourth slide sleeve (29) is connected with the upper surface of the conical shell (26), the top end of the third slide bar (25) is fixedly connected with the lower surface, the lower surface of the supporting plate (38) is fixedly connected with the upper surface of the conical shell (26) through a second electric push rod (39); a drainage pipe (30) is arranged on the lower surface of the conical shell (26), a three-way pipe (31) is arranged at the bottom end of the drainage pipe (30), a first check valve (32) and a second check valve (33) are arranged on the three-way pipe (31), one end of the three-way pipe (31) is communicated with a through hole (34), the through hole (34) is arranged on the upper surface of a fixing block (35), and a connecting shell (40) is arranged on the upper surface of the fixing block (35);
the inner surface of the connecting shell (40) is provided with internal threads, the surface of one end of the three-way pipe (31) is provided with external threads, the internal threads are connected with the external threads, and the front surface, the back surface, the left side surface and the right side surface of the fixing block (35) are provided with laser lamps (36); the lower surface fixed connection of fixed plate (12) has four blades (37), and four blades (37) enclose the city rectangle.
2. The gel excision device for DNA synthesis as claimed in claim 1, wherein the front face of the first movable plate (8) is clamped with a first sliding sleeve (10), the inner surface of the first sliding sleeve (10) is provided with a first sliding rod (9), and the two ends of the first sliding rod (9) are respectively fixedly connected with the opposite faces of the two connecting plates (2).
3. The gel excision device for DNA synthesis as claimed in claim 1, wherein the second movable plate (18) is connected with a second sliding sleeve (19) on its right side, the inner surface of the second sliding sleeve (19) is sleeved with a second sliding rod (20), and the two ends of the second sliding rod (20) are respectively fixedly connected with the opposite surfaces of the two fixed plates (12).
4. The gel excision device for DNA synthesis as claimed in claim 1, wherein the lower surface of the body of the first motor (4) is fixedly connected with the base (3), and the lower surface of the base (3) is fixedly connected with the upper surface of the base (1).
5. The gel excision device for DNA synthesis as claimed in claim 1, wherein the upper and lower surfaces of the second motor (14) body are fixedly connected to the opposite surfaces of the two fixing rods (13), respectively, and the left ends of the two second fixing rods (13) are fixedly connected to the right side surface of the right fixing plate (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022746017.5U CN214025854U (en) | 2020-11-25 | 2020-11-25 | Gel excision device for DNA synthesis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022746017.5U CN214025854U (en) | 2020-11-25 | 2020-11-25 | Gel excision device for DNA synthesis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214025854U true CN214025854U (en) | 2021-08-24 |
Family
ID=77361726
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022746017.5U Expired - Fee Related CN214025854U (en) | 2020-11-25 | 2020-11-25 | Gel excision device for DNA synthesis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214025854U (en) |
-
2020
- 2020-11-25 CN CN202022746017.5U patent/CN214025854U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210824 |
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| CF01 | Termination of patent right due to non-payment of annual fee |