CN106979878A - A kind of histopathology sample processing system - Google Patents
A kind of histopathology sample processing system Download PDFInfo
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- CN106979878A CN106979878A CN201710323633.XA CN201710323633A CN106979878A CN 106979878 A CN106979878 A CN 106979878A CN 201710323633 A CN201710323633 A CN 201710323633A CN 106979878 A CN106979878 A CN 106979878A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
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Abstract
The invention provides a kind of histopathology sample processing system, belong to technical field of medical instruments.Including removable grip unit, dehydration waxdip unit, section unit, slide glass dye unit, image scanning display unit and main control unit.Pass through each unit of the system, can be automatically performed the fixation from the histopathology sample after materials, dehydration, embedding, section, drift piece, dye and this series of processing steps of taking pictures, so as to reduce the amount of labour of technical staff, avoid technical staff from contacting poisonous and harmful substance, shorten the histopathology sample disposal time.
Description
Technical field
The present invention relates to technical field of medical instruments, and in particular to a kind of histopathology sample processing system.
Background technology
The premise of accurate pathological diagnosis is realized, is the processing tissue Pathologic specimen (hereinafter referred to as sample) of science, specification,
Making turns into qualified pathological section, and there is provided to pathologist.The processing procedure of sample, including draw materials, fix, being dehydrated, embedding,
Several key steps such as section, drift piece, dyeing, mounting.Wherein, dyeing can be divided into haematoxylin eosin stains, specific enzymes systematism
Learn dyeing, immunohistochemical staining, in situ hybridization etc..The implementation of these steps using a variety of with volatile, it is necessary to be had
Malicious reagent, has different degrees of harm to the health of technical staff.This is accomplished by a kind of supermatic equipment to reduce
Artificial operation, so that the health of safeguards technique personnel.
Pathological diagnosis is, it is necessary to not only accurate, and in time, more early acquisition pathological diagnosis is more conducive to the disease of patient to examine
Control.Traditional sample disposal process is, it is necessary to undergo 3-5 days, and even more long, its reason is：On the one hand it is each of sample
Step has clear and definite processing time to require, the time will not enough influence chipping qualities, so as to influence diagnosis；On the other hand it is each
Individual step is accomplished into before next step, and the time is wasted because of the factor such as the work handover of technical staff, rest.Cause
This is, it is necessary to a kind of supermatic equipment handles sample, on the premise of ensureing that each enough step process time, section
Save the time being connected between different step, it is to avoid the artificial time wastes, shorten the time completed required for sample disposal, so that
Patient is allowed to obtain pathological diagnosis earlier.
All processing procedures are undergone in sample, are made into after qualified histotomy, to be carried out by pathologist
Read, propose diagnosis.If desired other pathologists carry out remote medical consultation with specialists, then need that histotomy manually is put into digital pathology
IMAQ is carried out in slice scanner, digitized image is obtained, in order to remote transmission.If straight after the completion of microsection manufacture
Connecing scanning turns into digitized image, will save artificial operation, saves the time.
At present, there are substantial amounts of automatic dehydrator, automatic staining machine, automatic mounting machine and Full automatic digital pathological section
The equipment such as scanner are applied in real work, but these equipment only substitute staff and complete wherein some step, it is impossible to even
Continuous property completes to still need manually to be connected between all steps, different step, and some steps at present can only be by manually grasping
Make, such as cut into slices and drift piece.
If can realize that complete dynamicization of the steps such as piece, dyeing and IMAQ is fixed, be dehydrated, embedding, cutting into slices, being floated to sample
Operation, disclosure satisfy that the simpler reliable, requirement of higher efficiency of sample disposal.
The content of the invention
Technical problem of the present invention for solution in above-mentioned can be automatically performed tissue specimen handling process successively there is provided a kind of
Histopathology sample processing system.
The technical solution adopted in the present invention is：
A kind of histopathology sample processing system, including,
- grip unit is may move, for gripping sample and mobile tissue specimen or the carrier for being equipped with sample, including manipulator
With the guide rail moved for the manipulator, the end of the manipulator is rotary part and is provided with clamping jaw；
- dehydration waxdip unit, for being washed, being dehydrated by sample, transparent, waxdip and embedding, realizes and loads sample
In wax stone Riker mount and make and turn into wax stone, including dehydration waxdip mechanism, dehydration fluid cylinder group, for wax mechanism, the 3rd pipettor and
First thermoregulation mechanism；The dehydration waxdip mechanism be provided with some independent clamp dog cavitys, clamp dog cavity be provided with it is some
Wax stone Riker mount；The dehydration fluid cylinder group is used to deposit sample dehydration liquid and provided to dehydration waxdip mechanism, the confession wax machine
Structure is used to deposit liquid paraffin and provided to dehydration waxdip mechanism, and the 3rd pipettor is used to shift tissue specimen dehydration liquid；
The temperature control device is used for the melting and solidification for adjusting paraffin；
- section unit, for piece to be cut into slices and floated to the sample being embedded in wax stone Riker mount, obtains and comes from wax stone
The section of specimen embedding, including slicer pedestal, wax stone Riker mount clamping mechanism, section groove mechanism, the first travel mechanism and the
Two travel mechanisms；The forwardly convex formation boss in slicer pedestal stage casing, and be provided with for the longitudinally reciprocal movement of the boss
The first travel mechanism, the boss surface is provided with the wax stone clamping mechanism；The slicer pedestal bottom is extended forward
Above-mentioned boss section platform formed below, the section platform is gone back provided with section groove mechanism between section groove mechanism and platform surface
The second travel mechanism moved for the section groove mechanism transverse reciprocating is installed；
- slide glass dye unit, for the tissue specimen slide glass that section is completed in above-mentioned section unit to be carried out into roasting piece, dewaxing
And dyeing, including the first dyeing group and the second dyeing group；The first dyeing group includes the first dyeing chamber, second temperature regulation machine
Structure, the first dyeing fluid cylinder group and the first pipettor；The second dyeing group includes the second dyeing chamber, the second dyeing fluid cylinder group and the
Two pipettors；
- image scanning display unit, the image for gathering pathological section；
- Main Control Unit, for controlling the operation with electric device in the above-mentioned each unit of tissue treatment, and and external control
Acquisition terminal connection processed, including Master control chip and outer end connector.
Manipulator, guide rail and jaw shaped in the removable grip unit turn into six axis robot.
The clamping jaw pawl heart of the six axis robot is additionally provided with identification (mrfid) reader.
The wax stone Riker mount includes box body and sample carries groove, and the sample carries groove outwardly convex, by separable sample
Carry groove water jacket and sample carries groove inside groove and constitutes and mutually coincide, the groove face of the two is provided with fine and closely woven through hole, the tissue specimen
Lay and carried in sample between groove water jacket and sample load groove inside groove, and it treats that tangent plane is affixed to the water jacket that sample carries groove.
In the clamp dog cavity of the dehydration waxdip mechanism, axial order is provided with wax stone Riker mount carrier strip, the wax stone mark
This box order is installed on wax stone Riker mount carrier strip surface.
The wax stone Riker mount carrier strip is provided with preceding connection end and rear connection end, the two front frame respectively with the clamp dog cavity
It is rotatably connected with after-frame, and the preceding connection end forms rotating shaft to outer frame is prominent, the rotating shaft is provided with gear, adjacent wax stone mark
The gear intermeshing of this box carrier strip, and be provided with the gear positioned at the first wax stone Riker mount carrier strip for band moving gear
The stepper motor of rotation.
The confession wax mechanism includes, air compressor, electrically-controlled valve, wax storage and wax conveying tube, described in the wax conveying tube UNICOM
The clamp dog cavity and wax storage of waxdip mechanism are dehydrated, liquid paraffin flows between clamp dog cavity and wax storage.
The wax stone clamping mechanism includes clamping mouthful and push rod, and the clamping mouthful side is provided with opening, the wax stone sample
Box is inserted in clamping mouthful through the opening, and the clamping mouthful is longitudinally interference fit with wax stone Riker mount, the clamping mouthful and wax stone
Riker mount laterally coordinates for gap；The opposite side of the clamping mouthful is closing frame, is connected with push rod on the outside of the closing frame, institute
Closing frame is stated to be connected by elastomer with push rod.
The section groove mechanism, its groove face is ramp-like in dipping down, in addition to,
- blade apparatus, the front end upper surface of the section groove mechanism be horizontally arranged with blade and blade upward, simultaneously
The front end of the section groove mechanism is located at the cutter groove for filling blade and taking；
- left cut piece chamber, left cut piece chamber is provided with separable section fragment drawer；
- right cut piece chamber, is separated and separate with the left cut piece chamber by dividing plate, while the right cut piece chamber is divided into epicoele
Separate with cavity of resorption and by dividing plate, be provided with the left of the epicoele on the right side of slide platform, the epicoele and be equipped with slide glass folder, the slide glass
The cavity-like for intraformational bed is pressed from both sides, its front surface is provided with the scanning window for contaminating, its side is provided with the jaws entered for slide；Institute
The jaws for stating slide glass folder is corresponding with the slide platform surface slide lateral attitude；Slide scraper plate is provided with the left of the slide platform, is used
In the slide for being loaded with histotomy being pushed into the right in slide glass folder.- liquid bleeder, including the first flow export and second
Flow export, by solenoid valve control, the first flow export is embedded in the dividing plate between epicoele and cavity of resorption, for the liquid of epicoele to be evacuated to
In cavity of resorption, the second flow export is located at cavity of resorption bottom side, for the liquid of cavity of resorption to be released；
- intracavity liquid EGR, including feed tube, filter, water pump and drain pipe, the feed tube mouth of pipe are located at down
Intracavitary, for by water pump extract cavity of resorption in liquid, the filter be used for will enter feed tube liquid filter, it is described go out liquid
The pipe mouth of pipe is embedded in the dividing plate between epicoele and cavity of resorption, for the liquid extracted in cavity of resorption to be flowed out into epicoele by water pump
In；
- the three thermoregulation mechanism, the heating for liquid in the epicoele；
- slice probing device, including detection mouth, the detection mouthful is above the section groove mechanism.
The top and bottom of the slide scraper plate are fixing end, and its top and bottom is symmetrically connected with slide plate, and slide plate passes through
The slide rail transverse reciprocating movement of bottom；The slide platform bottom is additionally provided with slide shell fragment, for being upspring after top layer slide is pushed open
Next piece of new slide；Pinch roller is provided with above the slide platform, the pinch roller wheel bottom is fitted with slide, for being promoted in slide scraper plate
Slide is avoided to be moved perpendicular to slide direction during slide.
The cutter groove is electromagnet cutter groove.
Rule is provided with dye liquor tank in the first dyeing chamber, and slide glass folder is inserted in staining trough, the second temperature regulation
Mechanism includes calandria, for adjusting the first dyeing intracavity liquid temperature and roasting piece.
Mounting bar is pressed from both sides provided with slide glass in the second dyeing chamber, slide glass folder mounting bar is sequentially with some slide glass folders and installed
Mouthful.
Described image scans display unit, including sweep mechanism and image display.
First pipettor, the second pipettor or the 3rd pipettor, including liquid-transfering gun and liquid relief guide rail, the liquid relief are led
Rail is made up of X-axis liquid relief guide rail and Y-axis liquid relief guide rail.
A kind of histopathology sample processing system of the present invention, tissue specimen handling process can be automatically performed successively, its is excellent
Gesture is：
1st, the fixation from the histopathology sample after materials, dehydration, embedding, section, drift piece, dyeing can be automatically performed and schemed
As gathering this series of processing steps, and present invention omits mounting the step, directly slide glass folder is scanned and obtained
Digital picture, is conducive to the saving time；
2nd, full automation is operated, it is to avoid technical staff contacts poisonous and harmful reagent；
3rd, whole flow process is standardized, it is to avoid the difference between different slicing experiments, improves tissue pathological slice quality；
4th, each flow is closely connected, and shortens the histopathology sample disposal time；
5th, the pathological section digital picture finally obtained scan transfer at once, is easy to remote medical consultation with specialists.
Figure of description
Fig. 1 is the overall structure diagram of present system；
Fig. 2 is the structural representation of six axis robot；
Fig. 3 is the structural representation of dehydration waxdip mechanism；
Fig. 4 is the structural representation of wax stone Riker mount；
Fig. 5 is the structural representation for wax mechanism；
Fig. 6 is the overall structure diagram of section unit；
Fig. 7 is the structural representation of wax stone Riker mount clamping mechanism；
Fig. 8 is the structural representation of section groove mechanism；
Fig. 9 is the structural representation of right cut piece intracavitary liquid circulation；
Figure 10 is the structural representation of the first dyeing chamber；
Figure 11 is the structural representation of the second dyeing chamber；
Figure 12 is the structural representation of first (second, third) pipettor；
Figure 13 is the annexation figure of Main Control Unit.
Illustrate：
In Fig. 1,1-1 six axis robots, 2-1 dehydration waxdips unit, 3-1 sections unit, 4-1 slide glasses dye unit, 5-1 are swept
Retouch mechanism, 5-2 displays, the pipettors of 6-1 first, the pipettors of 6-2 second, the pipettors of 6-3 the 3rd, 7-1 dehydration fluid cylinders group, 7-2
First dyeing fluid cylinder group, the dyeing fluid cylinder groups of 7-3 second；
In Fig. 2,1-1 six axis robots, 1-2 clamping jaws, 1-3 identification (mrfid) readers；
In Fig. 3,2-2 dehydration waxdips mechanism, 2-3 clamp dogs cavity, 2-4 wax stone Riker mounts carrier strip, 2-5 gears, 2-6 steppings electricity
Machine, 2-7 wax stone Riker mounts；
In Fig. 4,2-7 wax stones Riker mount, 2-8 box bodys, 2-9 samples carry groove water jacket, 2-10 samples and carry groove inside groove；
In Fig. 5,2-11 air compressors, 2-12 electrically-controlled valves, 2-13 wax storages, 2-14 wax conveying tubes；
In Fig. 6,3-1 sections unit, 3-2 slicers pedestal, 3-3 wax stone Riker mounts clamping mechanism, the moving machines of 3-4 first
Structure, 3-5 sections groove mechanism, the travel mechanisms of 3-6 second, 3-27 slice probing devices；
In Fig. 7,3-3 wax stone Riker mounts clamping mechanism, 3-7 clampings mouth, 3-8 push rods, 3-9 springs；
In Fig. 8,3-5 sections groove mechanism, 3-10 blades, 3-11 electromagnet cutter groove, 3-12 left cuts piece chamber, 3-13 section fragments
Drawer, 3-14 right cuts piece chamber, 3-15 epicoeles, 3-16 cavity of resorptions, 3-17 slides platform, 3-18 slide glasses folder, 3-19 slides scraper plate, 3-20 pressure
Wheel；
In Fig. 9, the flow exports of 3-21 first, the flow exports of 3-22 second, 3-23 feed tubes, 3-24 filters, 3-25 water pumps, 3-
26 drain pipes；
In Figure 10, the dyeing of 4-2 first chamber, 4-3 dye liquor tanks, 3-18 slide glasses folder；
In Figure 11,4-4 slide glasses folder mounting bar, 4-5 slide glasses folder installing port, 3-18 slide glasses folder；
In Figure 12,6-4 liquid-transfering guns, 6-5X axle liquid reliefs guide rail, 6-6Y axle liquid relief guide rails.
Embodiment
Below in conjunction with the accompanying drawings, invention is further described.
Referring to Fig. 1, Fig. 1 of the present invention provides a kind of histopathology sample processing system, single including removable clamping
Member, dehydration waxdip unit 2-1, section unit 3-1, slide glass dye unit 4-1, image scanning collecting unit and main control list
Member.Removable grip unit is used to grip sample and mobile tissue specimen or the carrier for being equipped with sample, and dehydration waxdip unit 2-1 is used
In sample is washed, be dehydrated, transparent, waxdip and embedding, sample is fitted into wax stone Riker mount 2-7 and made by realization to be turned into
Wax stone.Section unit 3-1 is used for piece that the sample being embedded in wax stone Riker mount 2-7 is cut into slices and floated, and obtains and comes from wax stone
The section of middle specimen embedding.Slide glass dye unit 4-1 enters in by the tissue specimen slide glass that section is completed in above-mentioned section unit 3-1
The roasting piece of row, dewaxing and dyeing.Image scanning collecting unit is used for the image for gathering the histotomy after dyeing.Main control unit is used
The operation of electric device in control and the above-mentioned each unit of tissue treatment, and be connected with outer control acquisition terminal, including master control
Coremaking piece and outer end connector, its control chip and the control annexation of other each units are as shown in figure 13.
Removable grip unit as depicted in figs. 1 and 2, the unit is pressed from both sides using six axis robot 1-1 to section, slide glass
3-18 etc. is clamped and shifted, each position that six axis robot 1-1 may extend in processing system as shown in Figure 1.
Meanwhile, being dehydrated to tissue specimen, waxdip, during embedding, code mark can be identified to forming wax stone Riker mount 2-7
Note, so, in six axis robot 1-1 claw, identification (mrfid) reader 1-3 is additionally provided with, for reading coming for each tissue specimen of identification
The information such as source, are easy to record and the tracking of information.
Be dehydrated waxdip unit, by dehydration waxdip mechanism 2-2, dehydration fluid cylinder group 7-1, for wax mechanism, the 3rd pipettor 6-3 and
First thermoregulation mechanism is constituted.Waxdip mechanism 2-2 is dehydrated in dehydration waxdip mechanism 2-2 as shown in Figure 3, the present embodiment to be provided with
Three independent clamp dog cavity 2-3, adjacent clamp dog cavity 2-3 are separated by dividing plate.In each clamp dog cavity 2-3, axial order
It is provided with wax stone Riker mount carrier strip 2-4, wax stone Riker mount carrier strip 2-4 and is sequentially set with for filling the recessed of wax stone Riker mount 2-7
Fall into, shape and size and the sample of depression carry groove water jacket 2-9 and matched.Wax stone Riker mount carrier strip 2-4 is provided with preceding connection end with after
Connection end, the two is rotatably connected with clamp dog cavity 2-3 front frame and after-frame respectively, and the preceding connection end is to the prominent shape of outer frame
Into rotating shaft, the rotating shaft is provided with gear 2-5, adjacent wax stone Riker mount carrier strip 2-4 gear 2-5 intermeshings, and in positioned at head
Stepper motor 2-6 for being rotated with moving gear 2-5 is installed at the wax stone Riker mount carrier strip 2-4 of position gear 2-5.
Wax stone Riker mount 2-7 as shown in Figure 4, including box body 2-8 and sample carry groove, and wax stone Riker mount 2-7 includes box body
2-8 and sample carry groove, and sample carries groove outwardly convex, carry groove water jacket 2-9 by separable sample and sample carries groove inside groove 2-10 groups
Into and mutually coincide, the groove face of the two is provided with fine and closely woven through hole, and tissue specimen is laid carries groove water jacket 2-9 and sample is carried in sample
Between groove inside groove 2-10, and it treats that tangent plane is affixed to the water jacket that sample carries groove.Wax stone Riker mount 2-7 is made of plastic, in contact
Do not occur obvious deformation after the reagent for handling sample, the blade 3-10 that sample load slot part can be sliced machine is cut off easily；
It is put into after sample, sample carries groove inside groove 2-10 and compliance caves inward, so as to accommodate sample.
Dehydration fluid cylinder group 7-1 is made up of multiple equipped with tissue specimen dehydration with the band mouthful container of liquid, and sequentially proper alignment
Located at dehydration waxdip mechanism 2-2 sides, be dehydrated in tissue specimen, transparent step when, will be accordingly by the 3rd pipettor 6-3
Dehydration liquid suck and be transferred in dehydration waxdip mechanism 2-2 clamp dog cavity 2-3 to submerge each wax stone mark from place container
This box 2-7.
Confession wax mechanism as shown in Figure 5, including air compressor 2-11, electrically-controlled valve 2-12 and wax conveying tube 2-14, wax conveying tube
2-14 UNICOMs dehydration waxdip mechanism 2-2 clamp dog cavity 2-3 and wax storage 2-13, liquid paraffin is in clamp dog cavity 2-3 and storage wax
Flowed between tank 2-13.Liquid paraffin enters to be submerged after the wax stone Riker mount carrier strip 2-4 in clamp dog cavity 2-3, wax stone Riker mount
Carrier strip 2-4 is rotated to horizontal position, so can be after most of paraffin in making clamp dog cavity 2-3 returns to wax storage 2-13, still
There is part paraffin to retain in wax stone Riker mount carrier strip 2-4 depression, be reduced to below the melting point of paraffin, be then recessed when by temperature
In paraffin, so that wax stone is made.Paraffin is recycled, and is manually added in time when paraffin is not enough in wax storage 2-13.
Dehydration and transparent stage in tissue specimen, when wax stone Riker mount carrier strip 2-4 is in horizontal inclination position, install wax
The corresponding depressions of block Riker mount 2-7 will not residual liquid, i.e. each liquid effects in after tissue specimen certain time, it is necessary to complete
Remove entirely, can just add a kind of lower liquid, wax stone Riker mount carrier strip 2-4 is tilted, wax stone Riker mount 2-7 sample carries groove not
A kind of dehydration liquid of addition can be remained.When entering waxdip, embedding stage, wax stone Riker mount carrier strip 2-4 is in horizontal stroke
To horizontal position, the liquid with its volume respective amount can be retained in depression, now pass through and add paraffin to wax stone mark for wax mechanism
This box 2-7 sample is carried in groove, is flow back into wax storage 2-13 making the paraffin in addition to depression leave clamp dog cavity 2-3, is passed through
First thermoregulation mechanism reduction clamp dog cavity 2-3 temperature, solidifies wax, so as to be successfully made the wax containing tissue specimen
Block, then will be molded wax stone by six axis robot 1-1 and clamp and be transferred in the unit 3-1 of cutting into slices.
Section unit 3-1 as shown in figures 1 to 6, by slicer pedestal 3-2, wax stone Riker mount clamping mechanism 3-3, cuts
Piece groove mechanism 3-5, the first travel mechanism 3-4 and the second travel mechanism 3-6 compositions.As shown in fig. 7, in slicer pedestal 3-2
The forwardly convex formation boss of section, the first travel mechanism 3-4 for longitudinal direction can position limiting slide block and sliding surface, be installed on the boss and slicer
Pedestal 3-2 joint, makes boss longitudinally to reciprocatingly move.Boss surface is provided with wax stone clamping mechanism, wax stone clamping machine
Structure includes clamping mouthful 3-7 and push rod 3-8, clamping mouthful 3-7 sides and is provided with opening.During work, by six axis robot 1-1 by wax stone
Riker mount 2-7 is from clamp dog cavity 2-3 moves to opening insertion clamping mouthful 3-7, clamping mouthful 3-7 and wax stone Riker mount 2-7 longitudinal directions
For interference fit, the clamping mouthful 3-7 and wax stone Riker mount 2-7 laterally coordinates for gap, can both make wax stone Riker mount 2-7 upper
It will not be moved during lower section, can also be easy to wax stone Riker mount 2-7 is horizontal to pick and place.The clamping mouthful 3-7 opposite side is closing frame,
Push rod 3-8 is connected with the outside of the closing frame, closing frame is connected by a pair of symmetrical spring 3-9 with push rod 3-8, worked
In, when changing wax stone Riker mount 2-7, six axis robot 1-1 raps push rod 3-8, can be by wax stone Riker mount 2-7 from wax stone clamping
A part is ejected in mechanism, six axis robot 1-1 just easily can take out wax stone box.
Slicer pedestal 3-2 bottom is extended forward to be set on above-mentioned boss section platform formed below, the section platform
Have section groove mechanism 3-5, the second travel mechanism 3-6 for laterally can position limiting slide block and sliding surface, be installed on the section platform and section
Groove mechanism 3-5 joint, makes section groove mechanism 3-5 laterally to reciprocatingly move.Section groove mechanism 3-5 as shown in Figure 8, its
Groove face is slightly tilted down, and the mechanism includes blade 3-10 more changing devices, left cut piece chamber 3-12, right cut piece chamber 3-14, liquid flow
Go out device, intracavity liquid EGR, the 3rd thermoregulation mechanism and slice probing device 3-27.Before section groove mechanism 3-5
End upper surface be horizontally arranged with blade 3-10 and blade upward (one piece of blade of left cut piece chamber and each correspondence of right cut piece chamber), together
When section groove mechanism 3-5 front end be located at the electromagnet cutter groove 3-11 that blade 3-10 is filled and taken.During more allowing blade replacement 3-10, electromagnet
Cutter groove 3-11 powers off degaussing, is taken out old blade 3-10 by six axis robot 1-1, then new blade 3-10 is placed on into electromagnetism
In iron cutter groove 3-11；During work, electromagnet cutter groove 3-11 is powered and sucks blade 3-10, so as to carry out section work.Electromagnetism
Iron powers off degaussing, and manipulator removes the blade of left cut piece chamber, in the cutter groove that the blade of right cut piece chamber is placed on to left cut piece chamber,
Six axis robot takes out new blade in the cutter groove of right cut piece chamber from new blade receiver, now electromagnet energization will
Blade at two with changing holds installation.It is less demanding to blade because left cut piece chamber is used to slightly repair wax stone, and right cut piece chamber is used for
Histotomy is obtained, high is required to blade, it is necessary to sharp, N/D, therefore, in more allowing blade replacement, by the blade of right cut piece chamber
Left cut piece chamber recycling is put into, saving blade effect is played.
Left cut piece chamber 3-12 and right cut piece chamber 3-14 is separated and separate by dividing plate.Wax stone Riker mount 2-7 is loaded
After in section groove mechanism 3-5, before to its formal section, the sample in wax stone Riker mount 2-7 need to be repaired, i.e. wax stone mark
This box 2-7 protrusion outer layer excision, the tissue surface for being switched to tissue specimen is complete, so left cut piece chamber 3-12 be provided with it is separable and
The section fragment drawer 3-13 of replacing, when the sample in wax stone Riker mount 2-7 is repaired, the fragment cut will directly fall
Enter to cut into slices fragment drawer 3-13, section fragment drawer 3-13 cleanings or will be changed in cleaning, does not result in cut broken
It is in disorder scattered, it is difficult to clean out.3-14 points of right cut piece chamber is separated for epicoele 3-15 and cavity of resorption 3-16 and by dividing plate.Epicoele 3-
15 left side, which is provided with the right side of slide platform 3-17, epicoele 3-15, is equipped with slide glass folder 3-18, and slide glass folder is the cavity of intraformational bed
Shape, its front surface, which is provided with, to be used to contaminate the scanning window with IMAQ, and slide glass folder 3-18 uses transparent plastic material or glass
Material, with avoid next into staining procedure when be likely to result in by the effect of dyeing liquor burn into deformation etc., carry intermediate plate
3-18 scanning basis rest of the window towards epicoele, its side be provided with for slide enter jaws, slide glass folder 3-18 jaws with
Slide platform 3-17 surfaces slide lateral attitude correspondence.Slide scraper plate 3-19 is provided with the left of slide platform 3-17, slide scraper plate 3-19's
Top and bottom are fixing end, and its top and bottom is symmetrically connected with slide plate, and slide plate is moved by the slide rail transverse reciprocating of bottom,
Pressed from both sides for the slide for being loaded with histotomy to be pushed into the right into the slide glass in 3-18；Pinch roller 3- is provided with above the slide platform 3-17
20, pinch roller 3-20 are made up of 4 rubber wheels and are arranged with slide corner, and the wheel bottom of each rubber wheel is fitted with surface of glass slide, are used
Slide is avoided to be moved perpendicular to slide direction when in slide scraper plate 3-19 promotion slides；Slide platform 3-17 bottoms are additionally provided with slide
Shell fragment, for next piece of new slide of being upspring after top layer slide is pushed open.
As shown in figure 9, liquid bleeder includes the first flow export 3-21 and the second flow export 3-22, by magnetic valve control
System, the first flow export 3-21 is embedded in the dividing plate between epicoele 3-15 and cavity of resorption 3-16, for epicoele 3-15 liquid to be evacuated to down
In chamber 3-16, the second flow export 3-22 is located at cavity of resorption 3-16 bottom sides, for cavity of resorption 3-16 liquid to be released.Intracavity liquid is circulated
Device includes feed tube 3-23, filter 3-24, water pump 3-25 and drain pipe 3-26, and the feed tube 3-23 mouths of pipe are located at cavity of resorption 3-16
Interior, for extracting liquid in cavity of resorption 3-16 by water pump 3-25, filter 3-24 is used for the liquid mistake by feed tube 3-23 is entered
Filter, the drain pipe 3-26 mouths of pipe are embedded in the dividing plate between epicoele 3-15 and cavity of resorption 3-16, for will be extracted in cavity of resorption 3-16
Liquid is flowed out in epicoele 3-15 by water pump 3-25.The calandria of 3rd thermoregulation mechanism is located in epicoele 3-15.For
For the heating of liquid in epicoele 3-15.Slice probing device 3-27 is used to detecting and judging whether the drift piece of section reaches requirement,
For camera device, it detects mouth and camera above section groove mechanism 3-5, slice picture is shot and transimiison analysis.
During formal section, section groove is moved to left, right cut film trap is located at immediately below wax stone clamping mechanism, and by right cut piece chamber
After 3-14 epicoele 3-15 is full of water (controlling epicoele 3-15 water temperatures at 45 DEG C by the 3rd thermoregulation mechanism), soak liquid level
Do not have blade 3-10 below blade 1mm part.After wax stone Riker mount 2-7 histotomy is cut, water can be directly floated to
In, multiple histotomies of section are joined end to end, and the afterbody of finally cut a histotomy is ined succession blade.The tissue cut
The detected device monitoring of section, when the gauffer for finding histotomy disappears, that is, controls the first flow export 3-21 to make epicoele 3-15's
The water surface slowly declines, and the now histotomy dampening that will have some setbacks flows away, but is attached on slide downwards, then passes through six axis robot 1-
1 promotion slide scraper plate 3-19 pushes away to the right the slide with histotomy, in insertion slide glass folder 3-18, controls six axis robot 1-1
Slide glass folder 3-18 is taken out to be transferred in slide glass dye unit 4-1, and the slide glass more renewed by six axis robot 1-1 presss from both sides 3-
18.Now liquid circulating apparatus in transit chamber, the water conservancy that will transfer to cavity of resorption 3-16 enters feed tube 3-23 simultaneously with water pump 3-25
Come back in epicoele 3-15, now changed by slide shell fragment after filter 3-24 filterings, then by drain pipe 3-26
New slide, repeats above-mentioned section work.
Slide glass dye unit 4-1 is by the first dyeing group and the second dyeing group, wherein the first dyeing group is progress normal dyeing
Step and the dyeing group used, the second dyeing group is need to carry out specific stain (such as special enzyme staining, immuning tissue
Learn dyeing, in situ hybridization etc.) step and the dyeing group that uses.
First dyeing group as shown in Figure 10, including the first dyeing chamber 4-2, second temperature governor motion, the first dyeing liquor
Cylinder group 7-2 and the first pipettor 6-1.Rule is provided with multiple dye liquor tank 4-3 in first dyeing chamber 4-2, slide glass folder 3-18 insert in
In staining trough, second temperature governor motion includes calandria, and fluid temperature and roasting piece in chamber 4-2 are dyed for adjusting first, should
Calandria is the Resistant heating aluminium flake located at the first dyeing chamber 4-2.Slide glass folder 3-18 is gripped and inserted by six axis robot 1-1
In different dye liquor tank 4-3, dyeing liquor draws corresponding dyeing liquor by the first pipettor 6-1 from the first dyeing fluid cylinder group 7-2
Body is simultaneously delivered in corresponding dye liquor tank 4-3 slide glass folder 3-18, is again moved the dyeing liquor after use by first after the completion of conveying
Liquid device 6-1 is sucked back and is delivered to the first dyeing fluid cylinder group 7-2 original place, recycling, and dyeing is sequentially completed in this way.
Second dyeing group as shown in figure 11, including the second dyeing chamber, the second dyeing fluid cylinder group 7-3 and the second pipettor 6-
2.As shown in figure 12, mounting bar 4-4 is pressed from both sides provided with slide glass in the second dyeing chamber, slide glass folder mounting bar 4-4 is sequentially with some loads
Piece press from both sides installing port 4-5, due to the second dyeing group for carry out specific stain when use, general frequency of use with respect to the first dyeing group compared with
It is low, so slide glass folder mounting bar 4-4 slide glass folder 3-18 can be fitted into that quantity can be less than slide glass folder 3-18 in the first dyeing group can
Load quantity.When second dyeing group is dyed, the liquid for being added to slide glass folder progress tissue treatment does not return again to dyeing liquor cylinder group
7-3, but the vacuum suction hole pressed from both sides by being arranged at slide glass on mounting bar 4-4 will complete the liquid sucking-off after staining procedure, flow
To waste liquid cylinder.
Figure 12 is the first pipettor 6-1, the second pipettor 6-2, the 3rd pipettor 6-3 structural representation, by liquid relief
Rifle 6-4 and liquid relief guide rail composition, wherein liquid relief guide rail is made up of X-axis liquid relief guide rail 6-5 and Y-axis liquid relief guide rail 6-6, makes liquid-transfering gun
The top of any position of unit, is picked and placeed to related liquid where 6-4 is movable to.
As shown in figure 1, image scanning display unit, including sweep mechanism 5-1 and image display 5-2, and sweep mechanism
5-1 presss from both sides 3-18 delivery ports provided with slide glass.The slide glass folder 3-18 after dyeing is completed, can be by adding cedar oil in final step, can
The mounting step in conventional procedures is omitted, directly slide glass folder 3-18 is scanned and respective image is obtained, and shown in image
Shown in device 5-2.
Described above only to express the preferred embodiment of the present invention, it describes more specific and in detail, but can not be because
This and be interpreted as the limitation to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art,
On the premise of not departing from present inventive concept, some deformations can also be made, improves and substitutes, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (15)
1. a kind of histopathology sample processing system, it is characterised in that：Including,
- grip unit is may move, for gripping sample and mobile tissue specimen or the carrier for being equipped with sample, including manipulator and confession
The guide rail of manipulator movement, the end of the manipulator is rotary part and is provided with clamping jaw；
- dehydration waxdip unit, for being washed, being dehydrated by sample, transparent, waxdip and embedding, realizes sample loading wax stone
In Riker mount and make and turn into wax stone, including dehydration waxdip mechanism, dehydration fluid cylinder group, for wax mechanism, the 3rd pipettor and first
Thermoregulation mechanism；The dehydration waxdip mechanism, which is provided with some independent clamp dog cavitys, clamp dog cavity, is provided with some wax stones
Riker mount；The dehydration fluid cylinder group is used to deposit sample dehydration liquid and provided to dehydration waxdip mechanism, described for wax mechanism
There is provided in storage liquid paraffin and to dehydration waxdip mechanism, the 3rd pipettor is used to shift tissue specimen dehydration liquid；It is described
Temperature control device is used for the melting and solidification for adjusting paraffin；
- section unit, for piece to be cut into slices and floated to the sample being embedded in wax stone Riker mount, acquisition is embedded from wax stone
The section of sample, including slicer pedestal, wax stone Riker mount clamping mechanism, section groove mechanism, the first travel mechanism and the second shifting
Motivation structure；The forwardly convex formation boss in slicer pedestal stage casing, and be provided with for the longitudinally reciprocal movement of the boss
One travel mechanism, the boss surface is provided with the wax stone clamping mechanism；The slicer pedestal bottom extends forward above-mentioned
Boss section platform formed below, the section platform is also installed provided with section groove mechanism between section groove mechanism and platform surface
There is the second travel mechanism moved for the section groove mechanism transverse reciprocating；
- slide glass dye unit, for the tissue specimen slide glass that section is completed in above-mentioned section unit to be carried out into roasting piece, dewaxing and contaminated
Color, including the first dyeing group and the second dyeing group；The first dyeing group include first dyeing chamber, second temperature governor motion,
First dyeing fluid cylinder group and the first pipettor；The second dyeing group includes the second dyeing chamber, the second dyeing fluid cylinder group and second
Pipettor；
- image scanning display unit, the image for gathering pathological section；
- Main Control Unit, is adopted for controlling the operation with electric device in the above-mentioned each unit of tissue treatment, and with outer control
Collect terminal connection, including Master control chip and outer end connector.
2. a kind of histopathology sample processing system according to claim 1, it is characterised in that：The removable clamping is single
Manipulator, guide rail and jaw shaped in member turn into six axis robot.
3. a kind of histopathology sample processing system according to claim 2, it is characterised in that：The six axis robot
The clamping jaw pawl heart is additionally provided with identification (mrfid) reader.
4. a kind of histopathology sample processing system according to claim 1, it is characterised in that：The wax stone Riker mount bag
Include box body and sample carries groove, the sample carries groove outwardly convex, groove water jacket is carried by separable sample and sample carries groove inside groove group
Into and mutually coincide, the groove face of the two is provided with fine and closely woven through hole, and the tissue specimen is laid carries groove water jacket and sample in sample
Between load groove inside groove, and it treats that tangent plane is affixed to the water jacket that sample carries groove.
5. a kind of histopathology sample processing system according to claim 4, it is characterised in that：The dehydration waxdip mechanism
Clamp dog cavity in, axial order is provided with wax stone Riker mount carrier strip, and the wax stone Riker mount order is installed on wax stone Riker mount
Carrier strip surface.
6. a kind of histopathology sample processing system according to claim 5, it is characterised in that：The wax stone Riker mount is carried
Bar is provided with preceding connection end and rear connection end, and the two is rotatably connected with the front frame and after-frame of the clamp dog cavity respectively, and described
Preceding connection end forms rotating shaft to outer frame is prominent, and the rotating shaft is provided with gear, and the gear of adjacent wax stone Riker mount carrier strip is intermeshed,
And the stepper motor for driving pinion rotation is installed at the gear positioned at the first wax stone Riker mount carrier strip.
7. a kind of histopathology sample processing system according to claim 1, it is characterised in that：The confession wax mechanism bag
Include, air compressor, electrically-controlled valve, wax storage and wax conveying tube, the clamp dog cavity of waxdip mechanism is dehydrated described in the wax conveying tube UNICOM
And wax storage, liquid paraffin flows between clamp dog cavity and wax storage.
8. a kind of histopathology sample processing system according to claim 1, it is characterised in that：The wax stone clamping mechanism
Including clamping mouthful and push rod, the clamping mouthful side is provided with opening, and the wax stone Riker mount is inserted in clamping mouthful through the opening, institute
It is longitudinally interference fit that clamping mouthful, which is stated, with wax stone Riker mount, and the clamping mouthful laterally coordinates with wax stone Riker mount for gap；It is described
The opposite side of clamping mouthful is closing frame, and push rod is connected with the outside of the closing frame, and the closing frame passes through elastomer with push rod
Connection.
9. a kind of histopathology sample processing system according to claim 1, it is characterised in that：The section groove mechanism,
Its groove face is ramp-like in dipping down, in addition to,
- blade apparatus, the front end upper surface of the section groove mechanism be horizontally arranged with blade and blade upward, while described
The front end of section groove mechanism is located at the cutter groove for filling blade and taking；
- left cut piece chamber, left cut piece chamber is provided with separable section fragment drawer；
- right cut piece chamber, is separated and separate with the left cut piece chamber by dividing plate, while the right cut piece chamber is divided into epicoele with
Chamber is simultaneously separated by dividing plate, is provided with the left of the epicoele on the right side of slide platform, the epicoele and is equipped with slide glass folder, and slide glass folder is
The cavity-like of intraformational bed, its front surface, which is provided with, to be used to contaminate the scanning window with IMAQ, and its side is provided with what is entered for slide
Jaws；The jaws of the slide glass folder is corresponding with the slide platform surface slide lateral attitude；Slide is provided with the left of the slide platform
Scraper plate, for the slide for being loaded with histotomy to be pushed into the right in the slide glass folder；
- liquid bleeder, including the first flow export and the second flow export, by solenoid valve control, the first flow export is embedded in
In dividing plate between chamber and cavity of resorption, for the liquid of epicoele to be evacuated in cavity of resorption, the second flow export is located at cavity of resorption bottom side, for by under
The liquid of chamber is released；
- intracavity liquid EGR, including feed tube, filter, water pump and drain pipe, the feed tube mouth of pipe are located at cavity of resorption
It is interior, for by water pump extract cavity of resorption in liquid, the filter be used for will enter feed tube liquid filter, it is described go out liquid
The pipe mouth of pipe is embedded in the dividing plate between epicoele and cavity of resorption, for the liquid extracted in cavity of resorption to be flowed out into epicoele by water pump
In；
- the three thermoregulation mechanism, the heating for liquid in the epicoele；
- slice probing device, including detection mouth, the detection mouthful is above the section groove mechanism.
10. a kind of histopathology sample processing system according to claim 9, it is characterised in that：The slide scraper plate
Top and bottom are fixing end, and its top and bottom is symmetrically connected with slide plate, and slide plate is moved by the slide rail transverse reciprocating of bottom；
The slide platform bottom is additionally provided with slide shell fragment, for next piece of new slide of being upspring after top layer slide is pushed open；The slide
Pinch roller is provided with above platform, the pinch roller wheel bottom is fitted with slide, for avoiding slide perpendicular to glass when slide scraper plate promotes slide
Move in piece direction.
11. a kind of histopathology sample processing system according to claim 9, it is characterised in that：The cutter groove is electromagnetism
Iron cutter groove.
12. a kind of histopathology sample processing system according to claim 1, it is characterised in that：The first dyeing chamber
Middle rule is provided with dye liquor tank, and slide glass folder is inserted in staining trough, and the second temperature governor motion includes calandria, for adjusting
The first dyeing intracavity liquid temperature and roasting piece.
13. a kind of histopathology sample processing system according to claim 1, it is characterised in that：The second dyeing chamber
In press from both sides mounting bar provided with slide glass, slide glass folder mounting bar is sequentially with some slide glasses folder installing ports.
14. a kind of histopathology sample processing system according to claim 1, it is characterised in that：Described image scanning is aobvious
Show unit, including sweep mechanism and image display.
15. a kind of histopathology sample processing system according to claim 1, it is characterised in that：First pipettor,
Second pipettor or the 3rd pipettor, including liquid-transfering gun and liquid relief guide rail, the liquid relief guide rail are moved by X-axis liquid relief guide rail and Y-axis
Liquid guide rail is constituted.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710323633.XA CN106979878B (en) | 2017-05-09 | 2017-05-09 | Histopathological specimen processing system |
| PCT/CN2018/074373 WO2018205691A1 (en) | 2017-05-09 | 2018-01-27 | System for processing histopathological specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710323633.XA CN106979878B (en) | 2017-05-09 | 2017-05-09 | Histopathological specimen processing system |
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| Publication Number | Publication Date |
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| CN106979878A true CN106979878A (en) | 2017-07-25 |
| CN106979878B CN106979878B (en) | 2023-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710323633.XA Active CN106979878B (en) | 2017-05-09 | 2017-05-09 | Histopathological specimen processing system |
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| Country | Link |
|---|---|
| CN (1) | CN106979878B (en) |
| WO (1) | WO2018205691A1 (en) |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1232276A (en) * | 1968-05-22 | 1971-05-19 | ||
| US20010043884A1 (en) * | 1999-12-14 | 2001-11-22 | Ervin Essenfeld | Microwave unit and system for tissue processing |
| CN101004412A (en) * | 2006-01-20 | 2007-07-25 | 美国樱花检验仪器株式会社 | Automated system of processing biological specimens and method |
| US20080113440A1 (en) * | 2006-10-06 | 2008-05-15 | Leica Biosystems Melbourne Pty Ltd | Method and Apparatus for Tissue Sample Processing |
| CN102393321A (en) * | 2011-11-29 | 2012-03-28 | 熊宇杰 | Pregelatinized starch embedding medium used for tissue slices and production method thereof |
| CN202339295U (en) * | 2011-11-10 | 2012-07-18 | 罗东华 | Biological tissue treatment integrated machine |
| CN105628478A (en) * | 2016-01-26 | 2016-06-01 | 南昌德漫多科技有限公司 | Noval slice blanching instrument |
| CN105784991A (en) * | 2016-05-25 | 2016-07-20 | 南昌德漫多科技有限公司 | Device and method for repeated immunostaining of same tissue section |
| CN205981838U (en) * | 2016-08-30 | 2017-02-22 | 练亿香 | Integrated pathological tissue dehydration embedded box |
| CN206862721U (en) * | 2017-05-09 | 2018-01-09 | 南昌德漫多科技有限公司 | A kind of histopathology sample processing system |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5278252B2 (en) * | 2009-08-31 | 2013-09-04 | ソニー株式会社 | Tissue section image acquisition display device, tissue section image acquisition display method, and tissue section image acquisition display program |
| CN101915693B (en) * | 2010-07-07 | 2012-07-25 | 新疆医科大学 | Human embryonic trigeminus based three-dimensional reconstruction method by using slide stainer |
| US8879819B2 (en) * | 2012-12-19 | 2014-11-04 | Definiens Ag | Gleason grading by segmenting and combining co-registered images of differently stained tissue slices |
| US10281534B2 (en) * | 2014-03-20 | 2019-05-07 | Life Services, LLC | Tissue-slice MRI coil and rotation mechanism |
| CN105167795A (en) * | 2015-09-07 | 2015-12-23 | 胡漫 | PET/CT macroscopical digital information and pathological microscopic information matching method |
| CN106979878B (en) * | 2017-05-09 | 2023-12-15 | 贵阳德漫多医疗科技有限公司 | Histopathological specimen processing system |
-
2017
- 2017-05-09 CN CN201710323633.XA patent/CN106979878B/en active Active
-
2018
- 2018-01-27 WO PCT/CN2018/074373 patent/WO2018205691A1/en active Application Filing
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1232276A (en) * | 1968-05-22 | 1971-05-19 | ||
| US20010043884A1 (en) * | 1999-12-14 | 2001-11-22 | Ervin Essenfeld | Microwave unit and system for tissue processing |
| CN101004412A (en) * | 2006-01-20 | 2007-07-25 | 美国樱花检验仪器株式会社 | Automated system of processing biological specimens and method |
| US20080113440A1 (en) * | 2006-10-06 | 2008-05-15 | Leica Biosystems Melbourne Pty Ltd | Method and Apparatus for Tissue Sample Processing |
| CN202339295U (en) * | 2011-11-10 | 2012-07-18 | 罗东华 | Biological tissue treatment integrated machine |
| CN102393321A (en) * | 2011-11-29 | 2012-03-28 | 熊宇杰 | Pregelatinized starch embedding medium used for tissue slices and production method thereof |
| CN105628478A (en) * | 2016-01-26 | 2016-06-01 | 南昌德漫多科技有限公司 | Noval slice blanching instrument |
| CN105784991A (en) * | 2016-05-25 | 2016-07-20 | 南昌德漫多科技有限公司 | Device and method for repeated immunostaining of same tissue section |
| CN205981838U (en) * | 2016-08-30 | 2017-02-22 | 练亿香 | Integrated pathological tissue dehydration embedded box |
| CN206862721U (en) * | 2017-05-09 | 2018-01-09 | 南昌德漫多科技有限公司 | A kind of histopathology sample processing system |
Non-Patent Citations (2)
| Title |
|---|
| MICHAEL T MCCANN: "automated histology analysis：opportunities for signal processing", 《JOURNAL ＆ MAGAZINES》 * |
| 赵兵等: "前列腺针穿活检标本的标准化处理方法", 《临床与实验病理学杂志》 * |
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