CN112840195A

CN112840195A - Sample staining method, smear preparation equipment and staining solution combination

Info

Publication number: CN112840195A
Application number: CN201980066960.9A
Authority: CN
Inventors: 李学荣; 余珊
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2019-06-27
Filing date: 2019-06-27
Publication date: 2021-05-25
Anticipated expiration: 2039-06-27
Also published as: CN112840195B; WO2020258169A1

Abstract

Relates to a sample staining method, smear preparation equipment and a staining solution combination. The dyeing liquid combination comprises a first dyeing liquid and a second dyeing liquid, wherein the first dyeing liquid is a mixture of a first biological dye and a second biological dye, the first biological dye can dye acidophilic substances in a sample, and the second biological dye can dye alkalophilic substances in the sample. The second dye solution is different from the first dye solution, the second dye solution is a mixture of a third biological dye and a buffer solution, and the third biological dye can carry out redyeing on alkalophilic substances or acidophilic substances in the sample to enhance the dyeing effect. The sample dyeing method comprises the steps of soaking and dyeing a sample in a first dye solution, and then soaking and dyeing the sample in a second dye solution, wherein in the whole soaking process, the first dye solution and the second dye solution cannot lose efficacy in a short period, so that the first dye solution and the second dye solution can be used repeatedly, and the dyeing cost is saved.

Description

Sample staining method, smear preparation equipment and staining solution combination

Technical Field

The application relates to the field of medical detection, in particular to a sample dyeing mode.

Background

Sample staining is a very important step in the process of sample detection and analysis. Taking a slide as an example, the traditional slide staining method is basically completed by hands, and the efficiency is low. Therefore, many automated staining instruments are currently available. The automatic staining instruments generally adopt a vertical dip staining mode, the staining process basically imitates manual operation, usually in a small container, firstly pure staining solution is used for pre-fixing a blood membrane, and then mixed solution of the staining solution and buffer solution is prepared according to a certain proportion to soak a slide. After a period of time, the mixture was aspirated away and discarded, and then rinsed with deionized water.

Summary of The Invention

Technical problem

However, the mixed liquor of the dye liquor and the buffer liquor is ineffective within a short time, for example, about ten minutes, so that precipitation is generated, the dyeing effect is not provided any more, the mixed liquor cannot be recycled, and great waste is caused. Although small and medium containers in existing automated staining instruments are usually made into thin sheets to reduce the volume, the volume of the containers is still significantly larger than the amount of manual methods due to the influence of the area of a slide, so that the staining cost is still very high.

Solution to the problem

Technical solution

The application mainly provides a novel sample staining method, smear preparation equipment and a staining solution combination.

In one embodiment, the present application provides a sample staining method, including:

selecting: selecting a slide coated with a sample;

and (3) dyeing: taking out a sample after soaking in a staining tank, wherein the staining tank stores a first staining solution, the first staining solution is a mixture of a first biological dye and a second biological dye, the first biological dye can stain acidophilic substances in the sample, and the second biological dye can stain alkalophilic substances in the sample;

a secondary dyeing step: taking out a sample after soaking in a re-dyeing tank, wherein a second dyeing solution is stored in the re-dyeing tank, the second dyeing solution is different from the first dyeing solution, the second dyeing solution is a mixture of a third biological dye and a buffer solution, and the third biological dye can dye alkalophilic substances or acidophilic substances in the sample;

the method further comprises the following steps: and selecting the next slide coated with the sample, and repeating the staining step to the re-staining step.

In one embodiment, the method further comprises the step of dyeing promotion: soaking the sample treated by the dyeing step in a dyeing promotion tank, and then taking out the sample, wherein a buffer solution is stored in the dyeing promotion tank;

the dyeing promotion step is located after the dyeing step and before the counterdyeing step.

In one embodiment, in the step of accelerating, the sample is soaked in the buffer solution for 1-5 minutes.

In one embodiment, the method further comprises a cleaning step before the counterdyeing: and taking out the sample treated by the dyeing promotion step after soaking in a cleaning tank, wherein cleaning liquid is stored in the cleaning tank.

In one embodiment, the method further comprises a cleaning step before the counterdyeing: washing the sample with a cleaning liquid, the cleaning liquid being stored in a washing tank;

the pre-counterdyeing washing step is located after the dyeing step and before the counterdyeing step.

In one embodiment, in the pre-counterstaining washing step, the sample washing time is 1-5 minutes.

In one embodiment, the second dye liquor further comprises a first biological dye, the third biological dye is a basic biological dye, and the volume ratio of the first biological dye is far smaller than that of the third biological dye.

In one embodiment, the first biological dye is an eosin dye, the second biological dye is a methylene blue dye, and the third biological dye is an azure dye.

In one embodiment, the first dye liquor is a Ruhl-Giemsa dye liquor, the second dye liquor is a mixture of a Giemsa dye liquor and a buffer solution, or the second dye liquor is a mixture of a Liu's B dye liquor and a buffer solution.

In one embodiment, in the second dye solution, the volume ratio a of the giemsa dye solution or the liu's B dye solution to the buffer solution is 1/30-1/10.

In one embodiment, in the counterstaining step, the sample soaking time is 5 to 15 minutes.

In one embodiment, in the staining step, the sample soaking time is 1 to 5 minutes.

In one embodiment, the method further comprises a second cleaning step: and cleaning the sample treated by the counterstaining step.

In one embodiment, the method further comprises:

when the control unit judges that no sample needs to be dyed or receives a first dye liquor recovery instruction sent by a user, recovering the first dye liquor in the dyeing tank to a first dye liquor sealed container for storage, and waiting for next use;

and when the control unit judges that a sample needs to be dyed or receives a first dye liquor feeding instruction sent by a user, the first dye liquor stored in the first dye liquor sealed container is sent to the dyeing tank again so as to recycle the first dye liquor.

In one embodiment, the method further comprises:

when the control unit judges that no sample needs to be dyed or receives a first dye solution sealing instruction sent by a user, sealing the first dye solution in the dyeing tank for the next use;

and when the control unit judges that a sample needs to be dyed or receives a first dye solution opening instruction sent by a user, the control unit releases the sealing cover of the dyeing tank so as to recycle the first dye solution.

In one embodiment, the manner for the control unit to determine that no sample needs to be subjected to the staining step includes: no sample and no sample waiting to be fed into the staining bath, and/or the idle time of the staining bath exceeds a set value.

In one embodiment, the first dye liquor is discharged after the control unit judges that the first dye liquor reaches a set discharge requirement or receives a first dye liquor discharge instruction sent by a user.

In one embodiment, said discharge requirement comprises a ratio of the number of stains on the sample to the volume of the first stain reaching 2/ml or more and/or a usage time of said first stain reaching 2 days or more.

In one embodiment, the method further comprises:

when the control unit judges that no sample needs to be subjected to the re-dyeing step or receives a second dye liquor recovery instruction sent by a user, recovering the second dye liquor in the re-dyeing tank to a second dye liquor recovery container for storage, and waiting for next use;

and after the control unit judges that a sample needs to be subjected to the re-dyeing step or receives a second dye liquor feeding instruction sent by a user, re-sending the second dye liquor stored in the second dye liquor recovery container to a re-dyeing tank so as to recycle the second dye liquor.

In one embodiment, the method further comprises:

when the control unit judges that no sample needs to be subjected to the re-dyeing step or receives a second dyeing liquid sealing instruction sent by a user, sealing and covering the second dyeing liquid in the re-dyeing tank for the next use;

and when the control unit judges that a sample needs to be subjected to the secondary dyeing step or receives a second dyeing liquid opening instruction sent by a user, the control unit releases the sealing cover of the secondary dyeing tank so as to recycle the second dyeing liquid.

In one embodiment, the manner for the control unit to determine that no sample needs to be counterstained includes: the re-dyeing tank and the dyeing tank have no sample and no sample to be sent, and/or the idle time of the re-dyeing tank exceeds a set value.

In one embodiment, the second dye liquor is discharged when the control unit judges that the second dye liquor reaches a set discharge requirement or receives a second dye liquor discharge instruction sent by a user.

In one embodiment, the method further comprises:

when the control unit judges that no sample needs to be subjected to the cleaning step before counterstaining or receives a sealing cover cleaning liquid instruction sent by a user, sealing the cleaning liquid in the cleaning tank, and waiting for the next use;

and when the control unit judges that a sample needs to be subjected to the cleaning step before the counterstaining or receives a cleaning liquid opening instruction sent by a user, the control unit removes the cover of the cleaning tank so as to recycle the cleaning liquid.

In one embodiment, the method further comprises:

when the control unit judges that no sample needs to be subjected to the dyeing promotion step or receives a cover-sealing dyeing promotion liquid instruction sent by a user, the buffer solution in the dyeing promotion groove is covered for the next use;

and when the control unit judges that the sample needs to be subjected to the dyeing promotion step or receives a dyeing promotion liquid opening instruction sent by a user, the control unit releases the sealing cover of the dyeing promotion groove so as to recycle the buffer solution.

The application provides in an embodiment a smear preparation facility, including storage tank, transport mechanism and the control unit, the storage tank includes:

a staining bath for storing a first staining solution, the first staining solution being a mixture of a first biological dye and a second biological dye, the first biological dye being capable of staining eosinophilic substances in the sample, the second biological dye being capable of staining basophilic substances in the sample, the staining bath having a first opening through which a slide coated with the sample can pass;

a counterstaining bath for holding a second staining solution different from the first staining solution, the second staining solution being a mixture of a third biological dye and a buffer, the third biological dye being capable of staining an alkalophilic substance or an alkalophilic substance in the specimen, the counterstaining bath having a second opening through which the slide can pass;

the conveying mechanism is used for picking up the slide and driving the slide to move;

the control unit outputs a control signal to control the conveying mechanism to drive the sample to be soaked in the dyeing tank for dyeing firstly, and then soaked in the dyeing tank for dyeing again.

In one embodiment, the dyeing apparatus further comprises a first dye liquor path control system and a first dye liquor sealed container, wherein the first dye liquor path control system is respectively communicated with the first dye liquor sealed container and the dyeing tank, and is used for recycling the corresponding first dye liquor in the dyeing tank to the first dye liquor sealed container and discharging the first dye liquor in the first dye liquor sealed container to the dyeing tank again.

In one embodiment, the first dye liquor path control system comprises a first dye liquor recovery system and a first dye liquor inlet system, wherein the first dye liquor recovery system is respectively communicated with the first dye liquor sealed container and the dyeing tank and is used for recovering the corresponding first dye liquor in the dyeing tank to the first dye liquor sealed container; and the first dye liquor inlet system is respectively communicated with the first dye liquor sealed container and the dyeing tank and is used for discharging the first dye liquor in the first dye liquor sealed container into the dyeing tank again.

In one embodiment, the first dye liquor recovery system comprises a first dye liquor recovery pipeline and a first dye liquor recovery pressure source, the first dye liquor recovery pipeline is respectively communicated with the dyeing tank and the first dye liquor sealed container, and the first dye liquor recovery pressure source pumps the first dye liquor in the dyeing tank into the first dye liquor sealed container;

the first dye liquor inlet system comprises a first dye liquor inlet pipeline and a first dye liquor inlet pressure source, the first dye liquor inlet pipeline is respectively communicated with the dyeing tank and the first dye liquor sealed container, and the first dye liquor in the first dye liquor sealed container is discharged into the dyeing tank by the first dye liquor inlet pressure source.

In one embodiment, the first dye liquor recovery pipeline and the first dye liquor inlet pipeline are the same pipeline, and/or the first dye liquor inlet pressure source and the first dye liquor recovery pressure source are the same pressure source.

In one embodiment, after the control unit determines that no sample needs to be dyed or receives a first dye liquor recovery instruction sent by a user, the first dye liquor path control system recovers a corresponding first dye liquor in the dyeing tank to a first dye liquor sealed container; and when the control unit judges that a sample needs to be dyed or receives a first dye liquor inlet instruction sent by a user, the first dye liquor path control system discharges the first dye liquor in the first dye liquor sealed container into the dyeing tank again.

In one embodiment, the first dye liquor sealed container is a reagent bottle, which is arranged inside the smear preparation apparatus.

In one embodiment, the dyeing apparatus further comprises a first dye liquor cover, and after the control unit judges that no sample needs to be dyed or receives a first dye liquor instruction sent by a user for covering the dyeing tank, the first dye liquor cover shields a corresponding opening on the dyeing tank, and the dyeing tank is reopened when the dyeing tank needs to be used.

In one embodiment, the manner of determining that no sample needs to be stained by the control unit includes: the staining bath has no sample and no sample to be stained is fed in, and/or the staining bath idle time exceeds a set value.

In one embodiment, the dye liquor discharge system further comprises a first dye liquor discharge system, the first dye liquor discharge system is communicated with the dye vat or the first dye liquor sealed container, and when the control unit judges that the first dye liquor meets the discharge requirement or receives a first dye liquor discharge instruction sent by a user, the first dye liquor discharge system discharges the first dye liquor in the dye vat or the first dye liquor sealed container.

In one embodiment, the emission requirements include: the ratio of the dyeing times of the sample to the volume of the first dye liquor reaches more than 2 times/ml and/or the using time of the first dye liquor reaches more than 2 days.

In one embodiment, the system further comprises a second dye liquor path control system and a second dye liquor recovery container, wherein the second dye liquor path control system is communicated with the second dye liquor sealing container and the re-dyeing tank and is used for recovering the corresponding second dye liquor in the re-dyeing tank to the second dye liquor recovery container and discharging the second dye liquor in the second dye liquor recovery container to the re-dyeing tank again.

In one embodiment, the second dye liquor path control system comprises a second dye liquor recovery system and a second dye liquor inlet system, wherein the second dye liquor recovery system is communicated with the second dye liquor recovery container and the re-dyeing tank and is used for recovering the corresponding second dye liquor in the re-dyeing tank to the second dye liquor recovery container; and the second dye liquor inlet system is communicated with the second dye liquor recovery container and the re-dyeing tank and is used for discharging the second dye liquor in the second dye liquor recovery container into the re-dyeing tank again.

In one embodiment, the second dye liquor recovery system comprises a second dye liquor recovery pipeline and a second dye liquor recovery pressure source, the second dye liquor recovery pipeline is respectively communicated with the secondary dye vat and the second dye liquor recovery container, and the second dye liquor recovery pressure source pumps the second dye liquor in the secondary dye vat into the second dye liquor recovery container;

the second dye liquor inlet system comprises a second dye liquor inlet pipeline and a second dye liquor inlet pressure source, the second dye liquor inlet pipeline is respectively communicated with the secondary dyeing tank and the second dye liquor recovery container, and the second dye liquor in the second dye liquor recovery container is discharged into the secondary dyeing tank by the second dye liquor inlet pressure source.

In one embodiment, the second dye liquor recovery pipeline and the second dye liquor feeding pipeline are the same pipeline, and/or the second dye liquor feeding pressure source and the second dye liquor recovery pressure source are the same pressure source.

In one embodiment, after the control unit determines that no sample needs to be subjected to the re-dyeing work or receives a second dye liquor recovery instruction sent by a user, the second dye liquor path control system recovers a corresponding second dye liquor in the re-dyeing tank to a second dye liquor recovery container;

and when the control unit judges that a sample needs to be subjected to the re-dyeing work or receives a second dye liquor inlet instruction sent by a user, the second dye liquor path control system discharges the second dye liquor in the second dye liquor recovery container into the re-dyeing tank again.

In one embodiment, the system further comprises a second dye liquor cover, and after the control unit judges that no sample needs to be subjected to re-dyeing work or receives a second dye liquor covering instruction sent by a user, the control unit controls the second dye liquor cover to shield the re-dyeing tank; and when the control unit judges that a sample needs to be subjected to the re-dyeing work or receives a second dye liquor opening instruction sent by a user, controlling the second dye liquor cover to open the re-dyeing tank.

In one embodiment, the containing groove further comprises a dye promotion groove for storing buffer solution, and the dye promotion groove is provided with a third opening for the slide to pass through; the control unit outputs a control signal to control the conveying mechanism to drive the sample to sequentially move into the dyeing tank for soaking and dyeing, the dye promotion tank for dyeing promotion and the dyeing tank for dyeing reduplication.

In one embodiment, the containing groove further comprises a cleaning groove for storing cleaning liquid, and the cleaning groove is provided with a fourth opening for the slide to pass through; after the sample is dyed in the dyeing promotion tank and before the sample is dyed in the dyeing promotion tank again, the control unit outputs a control signal to control the conveying mechanism to drive the sample to move into the cleaning tank for cleaning.

In one embodiment, the dyeing tank, the dyeing promotion tank, the cleaning tank and the re-dyeing tank are sequentially arranged in a queue.

In one embodiment, the containing groove further comprises a cleaning groove for storing cleaning liquid, and the cleaning groove is provided with a fourth opening for the slide to pass through; the control unit outputs a control signal to control the conveying mechanism to drive the sample to sequentially move to the dyeing tank for soaking and dyeing, the cleaning tank for cleaning and the dyeing tank for dyeing again.

In one embodiment, the washing device further comprises a cleaning liquid cover, and after the control unit judges that no sample needs to be washed before counterstaining or receives a cover sealing cleaning liquid command sent by a user, the cleaning liquid cover is controlled to shield a corresponding opening on the washing tank, and the washing tank is reopened when the washing device needs to be used.

In one embodiment, the dyeing promoting device further comprises a dyeing promoting liquid cover, and after the control unit judges that no sample needs to be subjected to dyeing promoting work or receives a cover sealing dyeing promoting liquid instruction sent by a user, the control unit controls the dyeing promoting liquid cover to shield a corresponding opening on the dyeing promoting tank and reopens the dyeing promoting tank when the dyeing promoting tank needs to be used.

In one embodiment, the device further comprises a mounting seat and a first driving assembly, wherein the accommodating groove is mounted on the mounting seat, the mounting seat is in transmission connection with the first driving assembly, and the accommodating groove on the mounting seat moves under the driving of the first driving assembly.

In one embodiment, the container further comprises an integral outer cover, and the integral outer cover is used for shielding the corresponding opening on the containing groove when the containing groove is in the non-use state.

In one embodiment, the portable electronic device further comprises a mounting seat and a first driving assembly, wherein the accommodating groove is mounted on the mounting seat, and any one of the integral outer cover and the mounting seat is in transmission connection with the first driving assembly, so that the integral outer cover and the accommodating groove on the mounting seat move relatively under the driving of the first driving assembly, and the corresponding opening on each accommodating groove is shielded.

In one embodiment, the liquid injection device further comprises a liquid inlet system, and a liquid outlet of the liquid inlet system is communicated with the accommodating groove and used for injecting corresponding liquid into the accommodating groove.

The application provides a dyeing liquor combination for sample dyeing in an embodiment, including first dye liquor and second dye liquor, first dye liquor is the mixture of first biological dye and second biological dye, first biological dye can dye acidophilic substance in the sample, second biological dye can dye alkalophilic substance in the sample, the second dye liquor is the mixture of third biological dye and buffer, third biological dye can dye alkalophilic substance or acidophilic substance in the sample.

In one embodiment, the second and third biological dyes are the same biological dye.

According to the above embodiment, a first dye solution and a second dye solution are provided, the first dye solution is a mixture of a first biological dye and a second biological dye, the first biological dye can dye acidophilic substances in a sample, and the second biological dye can dye alkalophilic substances in the sample. The second dye solution is different from the first dye solution, the second dye solution is a mixture of a third biological dye and a buffer solution, and the third biological dye can carry out redyeing on alkalophilic substances or acidophilic substances in the sample to enhance the dyeing effect. Firstly, a sample is soaked and dyed in a first dye solution, and then the sample is soaked and dyed in a second dye solution, so that the first dye solution and the second dye solution cannot lose efficacy in a short period in the whole soaking process, the first dye solution and the second dye solution can be reused, and the dyeing cost is saved.

Advantageous effects of the invention

Brief description of the drawings

Drawings

FIGS. 1 and 2 are schematic views showing the construction of a smear preparation apparatus according to an embodiment of the present application;

FIG. 3 is a schematic illustration of a process for preparing a smear in one embodiment of the present application;

FIGS. 4-7 are schematic illustrations of steps of several different embodiments of the sample staining methods of the present application;

fig. 8 is a schematic structural view of a receiving groove according to an embodiment of the present application;

FIG. 9 is a schematic view of a sample staining apparatus according to an embodiment of the present application;

fig. 10 is a schematic structural diagram of a sample staining apparatus according to another embodiment of the present application.

Examples of the invention

Modes for carrying out the invention

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The inventor of the invention has conducted intensive analysis and research on the reason that the existing dye liquor generates the precipitate, and finally finds that different dyes in the existing dye liquor can generate chemical reaction in a buffer solution environment, and further generate the precipitate. Taking the dyeing liquor of Rui's Giemsa as an example, the dyeing liquor of Rui's Giemsa contains two main dyes of eosin and methylene blue. After the dyeing liquor of Reishi Giemsa and the buffer liquor are mixed, in the buffer liquor system, the eosin dye is in negative charge, the methylene blue dye is in positive charge, the above-mentioned two dyes can produce reaction and produce precipitation, and said process is irreversible, so that the mixed liquor of Reishi Giemsa and buffer liquor can not be stored for a long time, and can be discarded after once use, so that it can result in large waste.

In this regard, the present embodiment provides a sample staining method for staining a sample. The sample includes, but is not limited to, microorganisms, blood, body fluids, and bone marrow fluid. In order to express the inventive concept of the present application more clearly, blood (slide) and a smear preparation apparatus for making the slide are described below as examples.

Referring to fig. 1 and 2 in combination, in one embodiment, a smear preparation apparatus is provided for smear preparation of samples such as, but not limited to, microorganisms, blood, body fluids, and bone marrow fluids. The smear preparation apparatus includes a sampling mechanism 1 for taking a sample, a slide loading mechanism 2 for moving a slide to a working line, a dropping mechanism 3 for loading the sample to the slide, a pushing mechanism 4 for smoothing the sample on the slide, a drying mechanism (not shown) for drying a blood film on the slide, and a staining mechanism 5 for staining the slide.

Referring to fig. 1 to 3, in an embodiment, when the sampling mechanism 1 performs sample extraction, the sample is first mixed, and then a sampling device (e.g. a sampling needle 31) in the sampling mechanism 1 is used to suck the sample, according to the difference of the sample container, the sample sucking may be a puncture sample sucking (the sample container has a cover, and the sampling device passes through the cover of the sample container), or an open sample sucking (the sample container is open, and the sampling device directly sucks the sample from the open portion). Blood sample information detection can be performed to obtain information and comparison information, if necessary. In some embodiments, a micro-sampling mechanism 6 is further included, and the micro-sampling mechanism 6 can move the test tube placed by the operator directly towards the sampling device, or the sampling device can move the test tube placed by the operator towards the sampling device. In other implementation manners, the micro-sampling mechanism 6 can also directly move the test tube to the direction of the sample dropping mechanism 3, or the sample dropping mechanism 3 can also move to the direction of the test tube placed by the operator, and sample loading is directly carried out after the blood sample is sucked by the sample dropping mechanism 3 (for example, a blood dropping needle), so that the requirement of the blood sample can be reduced due to the fact that the blood is not required to be extracted by the sampling mechanism 1, and micro-sampling and preferential sampling are realized. When sampling is completed, the blood is ready to be dropped onto the slide via the dripping mechanism 3.

Accordingly, the slide loading mechanism 2 extracts the slide and loads the slide to the corresponding position to facilitate the blood dropping operation. In some embodiments, as shown in fig. 3, after the slide extraction operation is completed, operations such as slide left-right detection and slide cleaning may be performed, and then the slide is loaded to a set position. The loaded slide can be printed with relevant information, and the front and back detection of the slide and the like can be carried out at the same time.

Referring to fig. 1 to 3, after the blood dropping needle of the dropping mechanism 3 drops the sample onto the slide, the slide pushing mechanism 4 pushes the blood into a blood film shape on the slide. Generally, after the slide pushing operation is completed, the blood film on the slide is dried to stabilize the shape. As shown in fig. 3, in some embodiments, the slide may be driven to turn over before drying the blood membrane to meet the corresponding requirements. In some embodiments, the dried slide can be further subjected to a drying test to determine the drying effect of the blood film. In some embodiments, the dried slide may also be subjected to blood film development test to determine whether the blood film is developed and whether the development status meets the requirements.

Continuing with fig. 1-3, after the slide is pushed, the slide (blood smear) can be stained (which can be done by staining mechanism 5) or directly output (e.g., placed in slide basket 7 for output).

In view of the above-described smear preparation apparatus or other devices associated with sample staining, one embodiment of the present application provides a method for staining a sample.

Referring to fig. 4, 9 and 10, the sample staining method includes:

dyeing step S10: the sample is taken out after being immersed in the staining bath 110, and the staining bath 110 stores the first staining solution. The first dye solution is a mixture of a first biological dye and a second biological dye, the first biological dye can dye acidophilic substances in the sample, and the second biological dye can dye alkalophilic substances in the sample.

Counterdyeing step S30: the sample is taken out after being soaked in the counterstain groove 120, and the counterstain groove 120 stores the second dye solution. The second dye liquor is different from the first dye liquor, and the second dye liquor is a mixture of a third biological dye and a buffer solution. The third biological dye can stain alkalophilic or acidophilic substances in the sample. Wherein the third biological dye may be the same as or different from the first biological dye or the second biological dye.

The staining step S10 may stain the cytoplasm and nucleus of the specimen, respectively, to produce the desired effect. In one embodiment, in the staining step S10, the sample immersion time is 1-5 minutes (including 1 minute and 5 minutes, both inclusive referring to the time range). The soaking time may be set to 1-3 minutes in consideration of the comprehensive factors of the work efficiency and dyeing effect.

In the dyeing step S10, the first dye liquor is a mixture of a first biological dye and a second biological dye, which means that the first dye liquor mainly comprises the two dyes of the first biological dye and the second biological dye, and at the same time, auxiliary materials which are commonly used in the field can be added according to needs, and the auxiliary materials are conventional means in the field. Here, the first dye solution is not limited to contain only two components, i.e., the first biological dye and the second biological dye.

It will be understood by those skilled in the art that the first biological dye capable of staining the acidophilic substance in the sample means that the first biological dye can bind to the acidophilic substance in the sample to change the color. For example, the eosinophilic substance includes hemoglobin and eosinophilic granules, etc., and the first biological dye may be eosin dye, which binds to the eosinophilic substance to turn red. Similarly, the fact that the second biological dye can stain the alkalophilic substance in the sample means that the second biological dye can be combined with the alkalophilic substance in the sample to change the color. For example, the alkalophilic substance includes lymphocyte cytoplasm, alkalophilic particle, etc., and the second biological dye may be methylene blue dye capable of binding with the alkalophilic substance to become bluish-violet.

The nucleus mainly comprises DNA and alkaline protein, the alkaline protein is easy to combine with eosin to dye red, meanwhile, the nucleus contains a small amount of weakly acidic substances which combine with methylene blue to dye blue, but the nucleus is dyed to be purple red due to the low content and weak blue reaction. Azure, an oxide of methylene blue, may also play a similar role to methylene blue.

The specimen is immersed in a first staining solution, and a carrier (hereinafter, a slide glass is described as an example of a blood-carrying slide) with the specimen is immersed in the first staining solution for a certain period of time. The counterstaining step S30 can be used for staining the cell nucleus or the acidophilic substance or the alkalophilic substance in other positions of the sample again, and the addition of the buffer solution is favorable for improving the staining effect of the third dye on the acidophilic substance or the alkalophilic substance, so that the subsequent detection and observation are facilitated. In one embodiment, in the counterstaining step S30, the sample immersion time is 5-15 minutes. For example, the time can be controlled to about 10 minutes.

In the re-dyeing step S30, the second dye liquor is a mixture of a third biological dye and a buffer, which means that the second dye liquor mainly comprises two components of the third biological dye and the buffer, and at the same time, auxiliary materials which are commonly used in the field can be added according to needs, and are conventional in the field. Here, the second dye liquor is not limited to contain only the third biological dye and the buffer. The buffer solution refers to a liquid capable of providing an ionization environment, such as phosphate solution, and may even include an aqueous liquid capable of forming an ionization environment.

The counterdyeing step is used to reinforce the dyeing of the basophilic or acidophilic substance, the third biological dye can be chosen according to the actual needs, and can be, for example, the same as the first biological dye or the second biological dye, of course, the third biological dye can also be chosen from dyes different from the first biological dye and the second biological dye, for example, the first biological dye is eosin dye, the second biological dye is methylene blue dye, the third biological dye can be azure dye which can be oxidized from methylene blue dye and is used for dyeing the basophilic substance.

The dyeing method provides two kinds of dye solutions, a sample is soaked and dyed in a first dye solution, at the moment, no buffer solution is added into the first dye solution, and the first biological dye and the second biological dye do not have chemical reaction, so that no precipitation is generated. After the dyeing step is finished, the sample is soaked and dyed in a second dyeing solution, and at the moment, only one acid or alkaline biological dye (third biological dye) is added into the second dyeing solution and is used for dyeing alkalophilic substances or acidophilic substances in the sample. The third biological dye alone cannot form a precipitate in a buffer environment. In the whole dyeing process, the first dye liquor and the second dye liquor cannot lose efficacy in a short time, so that the first dye liquor and the second dye liquor can be used repeatedly, and the dyeing cost is saved. After the dyeing step S10, the carrier of the sample may carry a portion of the first bio-dye into the second dye solution, but the portion of the first bio-dye and the portion of the second bio-dye are very small and are not enough to form too much precipitate with the third bio-dye, and have a small effect on the second dye solution.

Although the number of the dye solutions is increased by adopting the first dye solution and the second dye solution for dyeing compared with the existing method, the first dye solution and the second dye solution can be repeatedly used for soaking a plurality of glass slides, and the timeliness is very long. On average, the cost of staining per slide is greatly reduced.

In the method, because the first dye liquor and the second dye liquor can be repeatedly used for a long time, one embodiment further comprises the following steps: and selecting the next slide coated with the sample, and repeating the dyeing step to the re-dyeing step. This step can be repeated for multiple uses.

Further, referring to fig. 5, 9 and 10, in an embodiment, the dyeing method further includes a dyeing promotion step S21: soaking the sample processed in the dyeing step S10 in a dye promotion tank 130, and then taking out the sample, wherein a buffer solution is stored in the dye promotion tank 130;

the dyeing promotion step S21 is located after the dyeing step S10 and before the counterdyeing step S30.

In the dyeing promotion step S21, the buffer solution can further promote the dyeing efficiency of the first and second biological dyes in the sample on the sample, and improve the dyeing effect. Meanwhile, the buffer solution plays a role in promoting the dyeing and also plays a role in cleaning. The slide glass after the dyeing promotion step S21 has fewer attached first and second biological dyes, which is beneficial to reducing the chemical reaction of the first and second biological dyes in the subsequent counterdyeing step S30.

In one embodiment, the sample is soaked in the buffer solution for 1-5 minutes, for example, about 2 minutes in the staining promotion step S21.

Further, referring to fig. 6, 9 and 10, in an embodiment, a cleaning step S22 before counterdyeing is further included: taking out the sample processed in the dyeing promoting step S21 after being soaked in the cleaning tank 140, wherein the cleaning liquid is stored in the cleaning tank 140;

the pre-counterdyeing washing step S22 is located after the promotion step S21 and before the counterdyeing step S30.

In this step, the cleaning liquid may be a buffer solution, a cleaning liquid, or the like, so as to soak or clean the sample. In the cleaning step S22 before counterstaining, the sample is cleaned with a buffer solution or a cleaning solution, so as to further reduce the amount of the first and second biological dyes carried on the slide, and after the treatment in the step S30, the slide hardly brings the first and second biological dyes into the second staining solution, thereby avoiding the chemical reaction of the first, second and third biological dyes to generate a precipitate, so that the second staining solution can be used for a longer time, and further reducing the staining cost. During cleaning, the sample can be soaked in a cleaning solution or a buffer solution, and the sample can also be washed by the cleaning solution or the buffer solution.

In the pre-counterstaining washing step S22, the sample may be washed with a buffer solution or a washing solution one or more times, depending on the washing effect and the requirement. The cleaning solution may be deionized water or other cleaning solutions commonly used in the art. Of course, buffers may be used to wash the sample and may further facilitate staining of the sample with dyes.

The washing time may be determined according to the washing effect and the purpose to be achieved, and in one embodiment, in the washing step S22 before counterstaining, the sample washing time is 1-5 minutes.

In another embodiment, as shown in fig. 7, 9 and 10, the dyeing promotion step S21 may not be performed, but the pre-counterdyeing washing step S22 may be performed after the dyeing step S10 and before the counterdyeing step S30. At this time, in the pre-counterstaining washing step S22, the sample is washed with a washing liquid, and the washing liquid is stored in the washing tank 140, and the washing liquid may be selected from a washing liquid and a buffer liquid (in this case, the pre-counterstaining washing step S22 corresponds to the aforementioned accelerating step S21).

The cleaning step S22 before counterstaining can clean the first and second biological dyes carried on the slide glass, and prevent the first and second biological dyes from being carried into the second staining solution and precipitating with the third biological dye.

Similarly, the washing time may be determined according to the washing effect and the objective to be achieved, and in one embodiment, in the washing step S22 before counterstaining, the sample washing time is 1-5 minutes.

As a more specific example, the third biological dye is a basic biological dye, which can stain alkalophilic substances in the sample, such as methylene blue or azure dye. The second dye liquor also comprises a first biological dye, and the volume ratio of the first biological dye is far smaller than that of the third biological dye, so that the two are prevented from generating precipitation due to chemical reaction.

Specifically, the first biological dye is an eosin dye, and the second biological dye is a methylene blue dye. Of course, the first and second biological dyes may be replaced by other dyes capable of staining both alkaliphilic and acidophilic substances, respectively.

Further, in order to satisfy the most clinical practice, in one embodiment, the first dye liquor is a morse-giemsa dye liquor, which includes eosin dye and methylene blue dye. The second dye liquor is formed by mixing Giemsa dye liquor and buffer liquor, and the Giemsa dye liquor contains methylene blue dye as third biological dye (namely the third biological dye is the same as the second biological dye).

The swiss dyeing liquid is prepared by mixing basic dye Methylene Blue (Methylene Blue) and acid dye Eosin (Eosin Y), and is also called Eosin-Methylene Blue dye. Wherein the colored portion of eosin is an anion, the colorless portion is a cation, and the colored portion is acidic, so that eosin is known as an acidic dye. Methylene blue is generally basic in chloride salt, with the colored portion being cationic and the colorless portion being anionic, as opposed to just eosin.

After a long time of dyeing liquor of Rue's, the methylene blue in it contains azure because of oxidation, and methylene blue, azure and eosin can dye the cell nucleus to be purple red, but can not dye the cytoplasm to be blue, and the redundant methylene blue can dye the cytoplasm to be blue.

The slide dyed by the method is very close to the traditional hand dyeing method of the Rui's-Giemsa dyeing liquid, has high doctor acceptance degree, does not change the habit of a microscopic doctor, and is easy to popularize.

The second dye liquor can be replaced by a mixture of other dye liquors with methylene blue dye and buffer solutions, such as Liu's B dye liquor.

Further, in order to improve the dyeing effect in the counterdyeing step S30, in the second dyeing solution, the volume ratio a of the Giemsa dyeing solution or the Liu' S B dyeing solution to the buffer solution is 1/30-1/10.

Preferably, in one embodiment, the volume ratio a of the giemsa stain or the liu B stain to the buffer solution in the second stain is 1/25.

Further, referring to fig. 4-7, in one embodiment, the method further includes a second cleaning step: the sample treated in the counterstaining step S30 was washed.

Similarly, the washing of the sample in this step may be performed using a washing solution or a buffer. The cleaning method may be a common cleaning method such as soaking or rinsing.

By adopting the dyeing method, the use cost of dyeing of a user can be greatly reduced, and the reagent in each step can be repeatedly used, so that the timeliness is very long, the reagent can be stored in the instrument for a long time without moving, and the liquid path structure is also very simple. In the limit condition, even the liquid path support is not needed, only the user can fill the reagent into the dyeing bath, the manufacturing cost of the whole instrument becomes very low, and the popularization in middle and low-end customers is facilitated.

The sample treated by the dyeing method can be further processed by drying and outputting, and these processes can be realized by the prior art, which is not described herein.

Further, since the present embodiment provides two kinds of dye solutions, both the first dye solution and the second dye solution are not deteriorated in a short period of time during the entire dyeing process, and the first dye solution and the second dye solution are repeatedly used.

In one embodiment, please refer to fig. 10, which further includes:

when the control unit judges that no sample needs to be dyed or receives a first dye liquor recovery instruction sent by a user, the first dye liquor in the dyeing tank 110 is recovered to the first dye liquor sealed container 710 for storage, and the next use is waited;

when the control unit determines that a sample needs to be dyed or receives a first dye liquor feeding instruction sent by a user, the first dye liquor stored in the first dye liquor sealed container 710 is sent to the dyeing tank 110 again, so that the first dye liquor can be reused.

Since the first dye liquor is usually methanol of high purity as a solvent, it has very strong volatility and will evaporate quickly when exposed to air. The first dye solution can be recovered, sealed and stored when not used in the steps so as to avoid volatilization and prolong the service life of the dye solution.

Of course, the above-mentioned steps are also implemented by other means besides recycling the dye liquor into the first dye liquor sealed container 710 for sealed preservation when not in use.

For example, in one embodiment, the method further comprises:

when the control unit judges that no sample needs to be dyed or receives a first dye solution sealing instruction sent by a user, sealing the first dye solution in the dyeing tank 110 for the next use;

when the control unit determines that a sample needs to be dyed or receives a first dye solution opening instruction sent by a user, the control unit releases the cover of the dyeing tank 110 to reuse the first dye solution.

The closure may be actuated by a corresponding actuating structure, either of which will be described below.

The above two ways are only an example of the sealed preservation of the first dye liquor, and the two ways can be selected and used according to the actual structure and the requirement. The two modes can reduce the volatilization of the first dye liquor, and can also avoid impurities in the equipment from falling into the first dye liquor, thereby avoiding the pollution of the first dye liquor.

For the timing of recycling or capping, in one embodiment, the manner of determining that no sample needs to be subjected to the staining procedure by the control unit includes, but is not limited to: no sample and no sample waiting to be fed into the staining bath 110, and/or the staining bath 110 idle time exceeds a set value.

The first dye liquor of the present application, because it can be reused, is much less frequently exchanged, in the simplest way, it can be exchanged manually by the user when it needs to be exchanged. Of course, in order to improve the working efficiency and further realize automation, in an embodiment, after the control unit judges that the first dye liquor reaches the set discharge requirement or receives a first dye liquor discharge instruction sent by a user, the first dye liquor is discharged. This discharge can be effected by means of a corresponding first dye liquor discharge system, which will be described further below. The discharged first dye liquor can be collected and treated uniformly.

The discharge requirement of the first dye liquor can be set according to actual requirements, and in one embodiment, the discharge requirement includes but is not limited to: the ratio of the dyeing times of the sample to the volume of the first dye liquor reaches more than 2 times/ml and/or the using time of the first dye liquor reaches more than 2 days. For example, when 50 ml of the first dye solution is stored in the dyeing tank, the first dye solution can be discharged after the sample is subjected to the dyeing operation in the first dye solution for 100 times or more. The dyeing times are not absolutely equal to the number of dyed samples, and when the sample completes one complete dyeing movement in the first dyeing solution as one dyeing, the same sample may be dyed in the first dyeing solution for many times due to different dyeing requirements or dyeing effects. When the first dye liquor meets the requirements, the first dye liquor can be discharged and replaced manually or automatically by a user.

Furthermore, the second dye solution, the cleaning solution, the dye promotion solution and the like can be respectively provided with a recovery system or a capping means to realize recovery and utilization.

In one embodiment, please refer to fig. 10, which further includes:

when the control unit judges that no sample needs to be subjected to the re-dyeing step or receives a second dye liquor recovery instruction sent by a user, the second dye liquor in the re-dyeing tank 120 is recovered to a second dye liquor recovery container 720 for storage, and the next use is waited;

when the control unit judges that a sample needs to be subjected to a re-dyeing step or receives a second dye liquor feeding instruction sent by a user, the second dye liquor stored in the second dye liquor recovery container 720 is sent to the re-dyeing tank 120 again so as to recycle the second dye liquor.

In addition, in one embodiment, the method may also include:

when the control unit judges that no sample needs to be subjected to the re-dyeing step or receives a second dyeing liquid covering instruction sent by a user, covering the second dyeing liquid in the re-dyeing tank 120 for the next use;

when the control unit judges that a sample needs to be subjected to the secondary dyeing step or receives a second dyeing liquid opening instruction sent by a user, the cover of the secondary dyeing groove 120 is removed, so that the second dyeing liquid is reused.

Through the mode, can avoid volatilizing of second dye liquor with second dye liquor recycle, can also avoid in some debris fall the first dye liquor in the equipment simultaneously, avoid causing the pollution of first dye liquor to improve the life of second dye liquor.

In one embodiment, the manner for the control unit to determine that no sample needs to be counterstained includes, but is not limited to: neither the resale tank 120 nor the staining tank 110 has samples and no samples are to be fed in, and/or the idle time of the resale tank 120 exceeds a set value.

In the same way, for the second dye liquor, the frequency of replacement is greatly reduced because the second dye liquor can be reused, and in the simplest mode, the second dye liquor can be manually taken and placed by a user when the second dye liquor needs to be replaced. Of course, in order to improve the working efficiency and further realize automation, in an embodiment, after the control unit judges that the second dye liquor reaches the set discharge requirement or receives a second dye liquor discharge instruction sent by a user, the second dye liquor is discharged.

The discharge requirement may be referred to the discharge requirement of the first dye liquor, for example, determined from the ratio of the number of stains on the sample to the volume of the second dye liquor and/or the number of days of use of the second dye liquor. Of course, other emission requirements may be set.

Similarly, referring to fig. 10, an embodiment of the cleaning liquid and the buffer liquid as the cleaning liquid further includes:

when the control unit judges that no sample needs to be cleaned before counterstaining or receives a sealing cover cleaning liquid instruction sent by a user, sealing the cleaning liquid in the cleaning tank 140 for the next use;

when the control unit determines that there is a sample to be washed before the counterstaining or receives a cleaning liquid opening command from a user, the control unit removes the cover of the washing tank 140 to reuse the cleaning liquid.

For the buffer used in the accelerating step, in one embodiment, the buffer further comprises:

when the control unit judges that no sample needs to be subjected to the dyeing promotion step or receives a capping dyeing promotion liquid instruction sent by a user, capping the buffer liquid in the dyeing promotion tank 130 for the next use;

when the control unit judges that a sample needs to be subjected to a dyeing promotion step or receives a dyeing promotion liquid opening instruction sent by a user, the sealing cover of the dyeing promotion groove 130 is removed, so that the buffer liquid is recycled.

Since the volatility of the cleaning solution and the buffer solution is lower than that of the first dyeing solution and the second dyeing solution, the above recovery of the cleaning solution and the buffer solution can be selected as appropriate. In addition, the discharge and replacement of the cleaning and buffer solutions can also be carried out manually by the user or automatically by the apparatus, with reference to the treatment methods of the first and second dyeing liquors as described above.

Referring to fig. 8-10, an embodiment of the present application further provides a sample staining apparatus applied to a smear preparation apparatus, which includes a receiving slot 100, a conveying mechanism 200, and a control unit (not shown). The sample staining apparatus can be used as a sample staining solution module of a smear preparation device or other related devices.

The receiving tank 100 includes a dyeing tank 110 for receiving a first dyeing solution and a re-dyeing tank 120 for receiving a second dyeing solution.

The first dye liquor may be the first dye liquor as hereinbefore defined. The staining bath 110 has a first opening through which a carrier with a sample can pass. The second dye liquor may also be the second dye liquor as defined above. The transfection tank 120 has a second opening through which the carrier can pass.

The transport mechanism 200 is used to pick up the slide 500 with the sample and move the slide 500. The control unit outputs a control signal to control the transport mechanism to drive the sample to be soaked in the dyeing tank 110 for dyeing (after the dyeing step S10 is completed), and then soaked in the dyeing tank 120 for dyeing again (after the dyeing step S30 is completed).

The conveying mechanism 200 can use various power sources, such as a motor, a cylinder, a hydraulic cylinder, an electromagnetic drive, etc., as a power source, so as to move the slide 500. In one embodiment, referring to fig. 9 and 10, the transfer mechanism 200 includes a pick assembly 210 for picking up a slide 500 and a first drive assembly 220 (the pick assembly 210 and the first drive assembly 220 are shown in a simplified schematic view in fig. 9), the first drive assembly 220 is in driving connection with the pick assembly 210 to drive the pick assembly 210 to move.

As described above, the first driving assembly 220 may be driven by a motor, an air cylinder, a hydraulic cylinder, an electromagnetic drive, or the like. The pick assembly 210 can employ various types of robot configurations that can pick and release the slides 500. The first driving assembly 220 drives the picking assembly to move, for example, as shown in fig. 9, the first driving assembly 220 drives the picking assembly 210 to move along the y-axis and the z-axis, so as to drive the slide 500 to change positions in the two directions, so as to move to the position of each accommodating groove 100 for soaking. Alternatively, as shown in fig. 10, the first driving assembly 220 drives the picking assembly 210 to move along three directions of the x-axis, the y-axis and the z-axis.

Further, referring to fig. 9 and 10, in an embodiment, the containing groove 100 further includes a dye promotion groove 130 for storing a buffer solution, and the dye promotion groove 130 has a third opening for the slide 500 to pass through. After the sample is dyed in the dyeing tank 110 and before the sample is counterdyed in the counterdyeing tank 120, the control unit outputs a control signal to control the conveying mechanism to drive the sample to move to the dyeing promotion tank 130 for dyeing promotion and cleaning (the dyeing promotion step S21 is completed).

Further, referring to fig. 9 and 10, in an embodiment, the containing groove 100 further includes a washing groove 140 for storing a washing liquid or a buffer liquid, the washing groove 140 has a fourth opening for the slide 500 to pass through; after the sample is dyed and cleaned in the dyeing promoting tank 130 and before the sample is re-dyed in the re-dyeing tank 120, the control unit outputs a control signal to control the conveying mechanism 200 to drive the sample to move into the cleaning tank 140 for cleaning (step S22 of cleaning before re-dyeing is completed).

In order to improve the convenience of operation and the dyeing efficiency, as shown in fig. 9 and 10, in one embodiment, the dyeing bath 110, the dyeing promotion bath 130, the cleaning bath 140 and the dyeing reissue bath 120 are arranged in a row, for example, along the y-axis. The picking assembly 210 may sequentially perform corresponding operations through the receiving slots 100, and sequentially complete the steps. This arrangement allows the sample staining apparatus to be more compact, and also allows the movement of the pick-up assembly 210 to be shortened, thereby increasing staining efficiency. It is understood that the dyeing bath 110, the dye promotion bath 130, the wash bath 140 and the dye revival bath 120 may be integrally formed or may be separately provided.

In yet another embodiment, the dye promotion tank 130 may not be present. The containing groove 100 further comprises a cleaning tank 140 for storing cleaning solution, the cleaning tank 140 has a fourth opening for the slide 500 to pass through; after the sample is dyed in the dyeing tank 110 and before the sample is re-dyed in the re-dyeing tank 120, the control unit outputs a control signal to control the conveying mechanism 200 to drive the sample to move into the cleaning tank 140 for cleaning (step S22 of cleaning before the re-dyeing is completed). The wash tank 140 may be disposed directly between the dyeing tank 110 and the counterdyeing tank 120.

In the above embodiments, the washing tank 140 can be configured as one or more than one, so that the sample can be washed by using the buffer solution or the washing solution at one or more times, which depends on the washing effect and the requirement.

Further, the receiving groove 100 further includes a second receiving groove 150 for receiving a cleaning solution or a buffer solution, and the second receiving groove 150 has a fifth opening through which the slide 500 passes. After the sample is counterstained in the counterstaining tank 120, the control unit outputs a control signal to control the conveying mechanism 200 to drive the sample to move into the cleaning tank 140 for cleaning (completing the second cleaning step S50).

Further, referring to fig. 8, in an embodiment, a part or all of the accommodating tank 100 (including the dyeing tank 110, the re-dyeing tank 120, the dyeing promoting tank 130, the cleaning tank 140 and the second cleaning tank 150) may adopt the structure shown in fig. 8. The receiving chamber 100 stores therein a corresponding liquid (e.g., a first dye solution, a second dye solution, a buffer solution or a cleaning solution) and can dip the slide 500 coated with the sample. The receiving groove 100 includes at least one first mounting part 101, and the first mounting part 101 forms an insertion area 102 for inserting the slide. The corresponding (e.g., staining) process is performed by immersing the slide glass 500 mounted on the first mounting member 101 in a liquid (e.g., a staining solution) for a certain time in the receiving tank 100.

Further, referring to fig. 9 and 10, in one embodiment, the dyeing tank 110, the counterdyeing tank 120, the dyeing promotion tank 130, the cleaning tank 140 and the second cleaning tank 150 are sequentially arranged. The number of the picking assemblies 210 is more than one. For example, as shown in fig. 10, two picking assemblies 210 can work simultaneously, thereby improving the work efficiency.

The dyeing bath 110, the counterdyeing bath 120, the dyeing promotion bath 130, the washing bath 140 and the second washing bath 150 may be separated, i.e., all or part of them may be independently installed and moved (if having a moving function). In addition, as shown in fig. 9 and 10, the grooves can be fixedly assembled into a whole, and the grooves can be integrally movable.

In this embodiment, as shown in fig. 10, the picking assembly 210 can be driven by the first driving assembly 220 (including the X-axis motor 221, the Y-axis motor 222 and the Z-axis motor 223) to move in the three XYZ axes directions, so as to move, insert and take in and out the slide 500. Here, the width direction of the slide glass 500 inserted into the receiving groove 100 (i.e., the width direction of the insertion region) is the X direction, the arrangement direction of the slide glass 500 inserted into the receiving groove 100 (i.e., the thickness direction of the insertion region) is the Y direction, and the insertion direction of the slide glass 100, i.e., the up-down direction, is the Z direction.

Further, in one embodiment, the transport mechanism 200 includes Y-axis rails 2221 and Y-axis sliders 2222 in the Y-direction, X-axis rails 2211 and X-axis sliders 2212 in the X-direction, a Y-axis motor 222 and an X-axis motor 221. The X-axis motor 221 and the Y-axis motor 222 may be, for example, stepping motors or servo motors.

The Y-axis rail 2221 extends linearly in the Y direction and is fixed to the lower surface of the support member 230. The support member 230 may be a top wall portion of a cabinet of the sample staining apparatus or a beam member for support, etc. The Y-axis slider 2222 is attached to the lower surface side of the Y-axis rail 2221 so as to be movable along the Y-axis rail 2221. The Y-axis motor 222 moves the Y-axis slider 2222 in the Y direction through a corresponding transmission mechanism. The transmission mechanism may be, for example, a belt-pulley mechanism, a rack-and-pinion mechanism, or the like.

The X-axis rail 2211 extends linearly in the X direction and is fixed to the lower surface of the Y-axis slider 2222. The X-axis slider 2212 is attached to a lower surface side of the X-axis rail 2211 and is movable along the X-axis rail 2211. The X-axis motor 221 moves the X-axis slider 2212 in the X direction through a corresponding transmission mechanism.

A pair of Y-axis slider 2222, X-axis rail 2211, X-axis slider 2212, X-axis motor 221, and Y-axis motor 222 are provided, respectively. A pair of X-axis sliders 2212 have pickup assembly 210 mounted to one side of the underside thereof. Thus, the pick assemblies 210 can move independently in the X direction along the respective X-axis rails 2211. In addition, the picking assembly 210 can also move independently in the Y direction along the common Y-axis track 2221.

Further, in one embodiment, first drive assembly 220 includes a Z-axis motor 223 for picking assembly 210 (e.g., gripper 2232) and a drive mechanism 2231. The Z-axis motor 223 lifts and lowers the holder 2232 through the transmission mechanism 2231. The transmission mechanism 2231 may be a belt pulley mechanism, a rack and pinion mechanism, or the like.

In addition, some embodiments may further include a drying tub 160 and an air blowing unit 610. The air blowing unit 610 may generate an air flow for drying to blow the slide glass 500 located in the drying bath 160, thereby achieving drying of the slide glass 500. The air blowing unit 610 includes, for example, an electric fan that can forcibly blow air into an air passage inside the drying tub 160. The slide glass 500 is set on the second mounting part 161 inside the drying bath 160, and the air blowing unit 610 can dry the slide glass 500 by blowing air to the slide glass 500 continuously for a certain time. By providing the drying tank 160 and the air blowing unit 610, the slide 500 after the staining process can be dried quickly, and the time required for the staining process can be shortened. Similarly, the slide 500 can be inserted into and removed from the second mounting member 51 by the transfer mechanism 200, and thus the apparatus configuration can be simplified.

In one embodiment, a heater 620 is further provided to heat the air blown from the air blowing unit 610. The heater 620 is positioned between the air blowing unit 610 and the drying tub 160. The air sent from the air blowing unit 610 is heated by the heater 620 and then sent to the air passage in the drying tub 160 in a warm air state in which the temperature thereof is increased. This makes it possible to more rapidly dry the slide 500 after the staining process, and thus the time required for the staining process can be further shortened.

In the whole dyeing process, the first dye liquor and the second dye liquor cannot lose efficacy in a short time, and the first dye liquor and the second dye liquor are repeatedly used. In view of the volatility of the first dye solution, referring to fig. 10, an embodiment further includes a first dye solution path control system (not shown in detail, only schematically represented by corresponding lines, and the related dye solution path control systems described below are all represented in this manner) and a first dye solution sealed container 710, wherein the first dye solution path control system is respectively communicated with the first dye solution sealed container 710 and the dyeing bath 110, and is used for recycling the corresponding first dye solution in the dyeing bath 110 to the first dye solution sealed container 710 and discharging the first dye solution in the first dye solution sealed container 710 to the dyeing bath 110 again.

The first dye liquor path control system may include a first dye liquor recovery system and a first dye liquor inlet system, wherein the first dye liquor recovery system is respectively communicated with the first dye liquor sealed container 710 and the dyeing tank 110, and is configured to recover the corresponding first dye liquor in the dyeing tank 110 to the first dye liquor sealed container 710. The first dye liquor inlet system is respectively communicated with the first sealed dye liquor container 710 and the dyeing tank 110, and is used for discharging the first dye liquor in the first sealed dye liquor container 710 into the dyeing tank 110 again.

In the above embodiment, the first dye liquor recovery system and the first dye liquor feeding system are respectively used for realizing recovery and feeding control. In contrast, in other embodiments, the first dye liquor path control system may be an integrated system, which has not only the recycling function but also the liquor feeding function.

In one embodiment, the first dye liquor recovery system comprises a first dye liquor recovery pipeline in communication with the dyeing bath 110 and the first sealed dye liquor container 710, respectively, and a first dye liquor recovery pressure source in direct or indirect communication with the first dye liquor recovery pipeline. The first dye liquor recovery pressure source draws the first dye liquor in the dye vat 110 into the first sealed dye liquor container 710.

In one embodiment, the first dye liquor inlet system comprises a first dye liquor inlet pipeline and a first dye liquor inlet pressure source, the first dye liquor inlet pipeline is respectively communicated with the dyeing bath 110 and the first sealed dye liquor container 710, and the first dye liquor inlet pressure source can be directly or indirectly communicated with the first dye liquor inlet pipeline. The first dye liquor feed pressure source discharges the first dye liquor in the first sealed dye liquor container 710 into the dyeing bath 110.

The first dye liquor recovery pressure source and the first dye liquor feed pressure source can both use air pressure to drive the flow of liquor, although other ways of applying pressure are possible. In one embodiment, the first liquor recovery line and the first liquor feed line are the same line, and/or the first liquor feed pressure source and the first liquor recovery pressure source are the same pressure source. For example, the first dye liquor recovery pipeline and the first dye liquor inlet pipeline are the same pipeline, and the recovery and liquor inlet functions are realized by connecting with different pressure sources and matching with the open-close design of the pipelines.

In the recycling and liquor feeding time, in an embodiment, after the control unit determines that no sample needs to be dyed or receives a first dye liquor recycling instruction sent by a user, the first dye liquor path control system recycles the corresponding first dye liquor in the dyeing tank 110 to the first dye liquor sealed container 710.

When the control unit determines that a sample needs to be dyed or receives a first dye liquor inlet command sent by a user, the first dye liquor path control system discharges the first dye liquor in the first dye liquor sealed container 710 into the dyeing tank 110 again.

Because the first dye liquor can be reused, the first dye liquor sealed container 710 for storing the first dye liquor does not need to be too large, and only needs to basically meet the requirement of the use amount of each dyeing at least. In this case, the first sealed container 710 can be a reagent bottle with a small volume, and a large recycling bin or other containers are not needed. The reagent bottle may even be a primary reagent bottle for the first dye liquor (if the primary reagent bottle is present in the first dye liquor), so that the reagent bottle can be directly replaced when the first dye liquor needs to be replaced, without any further discharge operation. When the reagent bottle is used as the first dye liquor sealed container 710, the reagent bottle is small in size, in one embodiment, the reagent bottle can be arranged inside the smear preparation equipment, the first dye liquor sealed container 710 can be protected, and meanwhile, the compactness of the whole equipment can be still ensured due to the small size of the reagent bottle. And the structure such as the recovery bucket that adopts great, then be unfavorable for setting up it in equipment, will increase the volume of equipment.

Since the first dye liquor is usually methanol of high purity as a solvent, it has very strong volatility and will evaporate quickly when exposed to air. The structure can recover, seal and store the first dye solution when not in use, so as to avoid volatilization and prolong the service life of the dye solution.

In one embodiment, the dyeing apparatus further comprises a first dye liquor cover for shielding a corresponding opening of the dyeing bath 110 when the dyeing bath 110 is not in use, and for reopening the dyeing bath 110 when it is needed to be used.

The first dye liquor cover can be controlled independently, or can be controlled together with the cover body of other containing grooves (such as the dyeing groove 120). When not in use, the first dye liquor cover can close the dyeing tank 110, thereby avoiding volatilization of the first dye liquor.

For the timing of recycling or capping, in one embodiment, the manner of determining that no sample needs to be stained by the control unit includes, but is not limited to: the staining bath 110 has no sample and no sample to be stained is fed in, and/or the staining bath 110 is idle for more than a set value.

Further, for the first dye liquor, the frequency of replacement is greatly reduced, frequent replacement is not needed, and the labor intensity of operators can be reduced. In the simplest way, the first dye solution can be taken and placed manually by the user when it needs to be replaced.

Certainly, in order to improve the working efficiency and further realize automation, in an embodiment, the dyeing system further includes a first dye liquor discharging system, the first dye liquor discharging system is communicated with the dyeing tank 110 or the first dye liquor sealed container 710, and when the control unit determines that the first dye liquor meets the discharging requirement or receives a first dye liquor discharging instruction sent by a user, the first dye liquor discharging system discharges the first dye liquor in the dyeing tank 110 or the first dye liquor sealed container 710.

The discharge requirement of the first dye liquor can be set according to actual requirements, and in one embodiment, the discharge requirement includes but is not limited to: the ratio of the dyeing times of the sample to the volume of the first dye liquor reaches more than 2 times/ml and/or the using time of the first dye liquor reaches more than 2 days. For example, when 50 ml of the first dye solution is stored in the dyeing tank 110, the first dye solution can be discharged after the sample is subjected to dyeing operation in the first dye solution for 100 times or more. The dyeing times are not absolutely equal to the number of dyed samples, and when the sample completes one complete dyeing movement in the first dyeing solution as one dyeing, the same sample may be dyed in the first dyeing solution for many times due to different dyeing requirements or dyeing effects. When the first dye liquor meets the requirements, the first dye liquor can be discharged and replaced manually or automatically by a user. Because the dye liquor is generally high in cost, the cost can be greatly reduced by recycling and reusing the dye liquor.

Similar to the recycling of the first dye solution, in an embodiment, the system further includes a second dye solution path control system and a second dye solution recycling container 720, the second dye solution path control system is communicated with the second dye solution sealed container and the re-dyeing tank 120, and is used for recycling the corresponding second dye solution in the re-dyeing tank 120 to the second dye solution recycling container 720, and discharging the second dye solution in the second dye solution recycling container 720 to the re-dyeing tank 120.

Similarly, the second dye liquor path control system may include a second dye liquor recovery system and a second dye liquor inlet system, the second dye liquor recovery system is communicated with the second dye liquor recovery container 720 and the re-dyeing tank 120, so as to recover the corresponding second dye liquor in the re-dyeing tank 120 to the second dye liquor recovery container 720; the second dye liquor inlet system is communicated with the second dye liquor recovery container 720 and the re-dyeing tank 120, and is used for discharging the second dye liquor in the second dye liquor recovery container 720 into the re-dyeing tank 120 again.

Of course, in some embodiments, the second dye liquor path control system can also be an integrated system, which not only has the recycling function, but also has the liquor feeding function.

In one embodiment, the second dye liquor recovery system comprises a second dye liquor recovery pipeline and a second dye liquor recovery pressure source, the second dye liquor recovery pipeline is respectively communicated with the re-dyeing tank 120 and the second dye liquor recovery container 720, and the second dye liquor recovery pressure source is directly or indirectly communicated with the second dye liquor recovery pipeline. The second dye liquor recovery pressure source sucks the second dye liquor in the counterdyeing tank 120 into the second dye liquor recovery vessel 720.

The second dye liquor inlet system comprises a second dye liquor inlet pipeline and a second dye liquor inlet pressure source, the second dye liquor inlet pipeline is respectively communicated with the re-dyeing tank 120 and the second dye liquor recovery container 720, and the second dye liquor in the second dye liquor recovery container 720 is discharged into the re-dyeing tank 120 by the second dye liquor inlet pressure source.

The second source of dye liquor recovery pressure and the second source of dye liquor feed pressure may refer to the settings described above for the first source of dye liquor recovery pressure and the first source of dye liquor feed pressure. In one embodiment, the second dye liquor recovery pipeline and the second dye liquor feed pipeline are the same pipeline, and/or the second dye liquor feed pressure source and the second dye liquor recovery pressure source are the same pressure source.

The timing of the recovery and feeding of the second dye liquor may include, but is not limited to, the following:

when the control unit determines that no sample needs to be re-dyed or receives a second dye liquor recycling instruction sent by a user, the second dye liquor path control system recycles the corresponding second dye liquor in the re-dyeing tank 120 to the second dye liquor recycling container 720.

When the control unit judges that a sample needs to be subjected to the re-dyeing work or receives a second dye liquor inlet instruction sent by a user, the second dye liquor path control system discharges the second dye liquor in the second dye liquor recovery container 720 into the re-dyeing tank 120 again.

Similarly, in addition to the recovery, sealing and preservation, in an embodiment, a second dye solution cover may be further included, and when the control unit determines that no sample needs to be subjected to the re-dyeing work or receives a second dye solution instruction for capping, which is sent by a user, the second dye solution cover is controlled to shield the re-dyeing tank 120; and when the control unit judges that the sample needs to be subjected to the re-dyeing work or receives a second dye liquor opening instruction sent by a user, controlling a second dye liquor cover to open the re-dyeing tank 120.

In one embodiment, the method for the control unit to determine that no sample needs to be counterstained includes, but is not limited to: neither the resale tank 120 nor the staining tank 110 has samples and no samples are to be fed in, and/or the idle time of the resale tank 120 exceeds a set value.

Likewise, for the dye promotion tank 130, the rinse tank, and the second rinse tank 150, the recovery and intake of the liquid therein can also be achieved with reference to the recovery and intake system described above.

The recovery and feed systems corresponding to the dye promotion tank 130, the cleaning tank 140 and the second cleaning tank 150 can be realized by the same structure as that of the first dye liquor path control system and the first dye liquor sealed container 710. For example, the dye promotion tank 130 is communicated with the dye promotion liquid recovery container 730 through a dye promotion liquid path control system to realize recovery and liquid feeding. The cleaning tank 140 is communicated with the cleaning solution recovery container 740 through a cleaning solution path control system to realize recovery and liquid feeding. The second cleaning tank 150 is communicated with the second cleaning solution recovery container 750 through a second cleaning solution path control system, so as to realize recovery and liquid feeding.

Of course, the dye promotion tank 130, the cleaning tank 140 and the second cleaning tank 150 may be sealed by corresponding cover bodies.

In one embodiment, the dyeing promoting liquid cover is further included, and after the control unit determines that no sample needs to be subjected to dyeing promoting work or receives a cover closing dyeing promoting liquid instruction sent by a user, the control unit controls the dyeing promoting liquid cover to shield a corresponding opening on the dyeing promoting tank 130, and reopens the dyeing promoting tank 130 when the dyeing promoting tank needs to be used.

In one embodiment, the washing machine further comprises a cleaning liquid cover, and when the control unit determines that no sample needs to be washed before counterstaining or receives a cover-closing cleaning liquid command sent by a user, the control unit controls the cleaning liquid cover to shield a corresponding opening on the washing tank 140, and reopens the washing tank 140 when the washing machine needs to be used.

In one embodiment, the second cleaning tank 150 may also be provided with a corresponding second cleaning tank cover, and the second cleaning tank 150 is sealed under the control of the control unit.

The above recycling and liquid feeding systems and corresponding covers corresponding to the respective receiving grooves may be selected to be used according to actual requirements, and usually, the first dye solution and the second dye solution are preferentially recycled to reduce the dyeing cost.

The liquid feeding system and the recycling system corresponding to the dyeing tank 110, the re-dyeing tank 120, the dyeing promotion tank 130, the washing tank 140 and the second washing tank 150 may be used either or both. The pressure source for recovering and the pressure source for feeding liquid can be set separately, or the same pressure source can be used as the pressure source for sucking and feeding liquid.

In the case of the recovery system, each liquid may be recovered and stored after the dyeing work is completed every day (or every batch of samples), and then released into the corresponding receiving groove 100 when the sample is used next time. Of course, after the continuous use for a period of time, the liquid can be recovered and stored, so that the volatilization amount of the reagent is reduced and the pollution of the reagent is avoided. The reagents in the respective containers 100 may be replaced periodically or after staining a predetermined amount of the sample.

Further, referring to fig. 9, in addition to providing an independent cover body, in an embodiment, the sample staining apparatus further includes an integral cover 300, and the integral cover 300 is used for shielding the corresponding opening of each containing groove 100 when the containing grooves 100 are not in use. The non-use state may refer to a non-working state, that is, when the staining is not performed, or when the slide is not placed in one of the receiving grooves 100. The integral cover 300 can cover all the accommodating grooves 100 uniformly, for example, when a slide is not placed in one accommodating groove 100, or when the integral cover 300 covers the corresponding accommodating groove as mentioned above, the accommodating groove 100 can be covered by the integral cover 300.

Of course, the entire cover 300 may be manually covered on the receiving groove 100 by a worker, or may be designed to have a transmission structure to automatically cover the receiving groove 100.

In an embodiment, referring to fig. 9, the sample staining apparatus further includes a mounting base 400 and a second driving assembly (not shown), wherein the receiving groove 100 is mounted on the mounting base 400, and either one of the integral cover 300 and the mounting base 400 is in transmission connection with the second driving assembly, so that the integral cover 300 and the receiving groove 100 on the mounting base 400 move relatively under the driving of the second driving assembly, and the opening corresponding to each receiving groove 100 is shielded.

In one embodiment, the receiving cavity 100 is moved such that the integral cover 300 covers the receiving cavity 100. Wherein, each groove body in the containing groove 100 can move independently or move integrally.

In fig. 9, the second driving assembly is used to drive the mounting seat 400, and the entire cover 300 can be kept different and is located above the corresponding opening of each receiving groove 100. The second drive assembly may drive mount 400 to move along the x-axis. In operation, the integral cover 300 opens the corresponding opening of the container 100. When the receiving groove 100 needs to be shielded, the second driving assembly drives the mounting seat 400 to communicate with the receiving groove 100 and move toward the integral cover 300 until the integral cover 300 covers the corresponding opening of the receiving groove 100.

The sample staining apparatus can be used to realize the steps of the staining method, and the staining method is not limited to the apparatus shown in the present embodiment.

In another aspect, the present application further provides a staining solution combination for staining a sample, which includes a first staining solution and a second staining solution, wherein the first staining solution is a mixture of a first biological dye and a second biological dye, the first biological dye can stain an acidophilic substance in the sample, and the second biological dye can stain an alkalophilic substance in the sample. The second dye solution is a mixture of a third biological dye and a buffer solution, namely, the buffer solution and the third biological dye are mainly used as active ingredients in the second dye solution. The third biological dye is capable of staining for alkalophilic or eosinophilic substances in the sample, which may be the same as or different from the first biological dye or the second biological dye. The first, second and third biological dyes may be selected from the dyes described above.

In some embodiments, the second dye liquor may be a mixture of the first biological dye, the third biological dye and a buffer. For example, Giemsa dye liquor consists of azure (formed by oxidation of methylene blue) as the third biological dye, and eosin as the first biological dye, but the amount of azure is significantly greater than the amount of eosin. In which case the volume fraction of the first bio-dye is much smaller than the volume fraction of the third bio-dye, for example the ratio of the first bio-dye to the third bio-dye may be less than 1/3.

The staining solution combination can be used in, but not limited to, the staining method and the sample staining device, and can also be used in other staining methods and sample staining devices.

As a more specific example, the first biological dye is an eosin dye, the second biological dye is a methylene blue dye, and the third biological dye is an azure dye. Of course, the first, second and third biological dyes may be replaced by other dyes capable of staining acidophilic and alkaliphilic substances, respectively.

Further, to meet the most common usage habits of clinical departments, in one embodiment, the first dye liquor is a Reid-Giemsa dye liquor comprising an eosin dye and a methylene blue dye. The second dye liquor is a mixture of Giemsa dye liquor and buffer liquor, and the Giemsa dye liquor contains methylene blue dye as a third biological dye.

The present application has been described with reference to specific examples, which are provided only to aid understanding of the present application and are not intended to limit the present application. Variations of the above-described embodiments may occur to those of ordinary skill in the art in light of the teachings of this application.

Claims

A method of staining a sample, comprising:

selecting: selecting a slide coated with a sample;

and (3) dyeing: taking out a sample after soaking in a staining tank, wherein the staining tank stores a first staining solution, the first staining solution is a mixture of a first biological dye and a second biological dye, the first biological dye can stain acidophilic substances in the sample, and the second biological dye can stain alkalophilic substances in the sample;

a secondary dyeing step: taking out a sample after soaking in a re-dyeing tank, wherein a second dyeing solution is stored in the re-dyeing tank, the second dyeing solution is different from the first dyeing solution, the second dyeing solution is a mixture of a third biological dye and a buffer solution, and the third biological dye can dye alkalophilic substances or acidophilic substances in the sample;

the method further comprises the following steps: and selecting the next slide coated with the sample, and repeating the staining step to the re-staining step.
The method for staining a specimen according to claim 1, further comprising a step of accelerating the staining: soaking the sample treated by the dyeing step in a dyeing promotion tank, and then taking out the sample, wherein a buffer solution is stored in the dyeing promotion tank;

the dyeing promotion step is located after the dyeing step and before the counterdyeing step.
The method for staining a specimen according to claim 2, wherein in the step of accelerating, the specimen is soaked in the buffer for 1 to 5 minutes.
A method of staining a specimen according to claim 2 or 3, further comprising a washing step prior to counterstaining: and taking out the sample treated by the dyeing promotion step after soaking in a cleaning tank, wherein cleaning liquid is stored in the cleaning tank.
The method of staining a specimen according to claim 1, further comprising a pre-counterstaining washing step of: washing the sample with a cleaning liquid, the cleaning liquid being stored in a washing tank;

the pre-counterdyeing washing step is located after the dyeing step and before the counterdyeing step.
The method for staining a specimen according to claim 4 or 5, wherein in the washing before counterstaining step, the specimen washing time is 1 to 5 minutes.
The method for staining a specimen according to any of claims 1 to 6, further comprising a first biological dye in the second staining solution, wherein the third biological dye is a basic biological dye, and wherein the volume fraction of the first biological dye is much smaller than the volume fraction of the third biological dye.
The method for staining a specimen according to any one of claims 1 to 7, wherein the first biological dye is an eosin dye, the second biological dye is a methylene blue dye, and the third biological dye is an azure dye.
The method for staining a specimen according to any one of claims 1 to 6, wherein the first staining solution is a Reishi-Giemsa staining solution, the second staining solution is a mixture of a Giemsa staining solution and a buffer solution, or the second staining solution is a mixture of a Liu's B staining solution and a buffer solution.
The method for staining a specimen according to claim 9, wherein the volume ratio a of the giemsa or liu's B stain to the buffer in the second stain is 1/30 ≤ a ≤ 1/10.
The method for staining a specimen according to any one of claims 1 to 10, wherein, in the counterstaining step, the specimen is soaked for 5 to 15 minutes.
The method for staining a specimen according to any one of claims 1 to 11, wherein in the staining step, the specimen is soaked for 1 to 5 minutes.
The method for staining a specimen according to any one of claims 1 to 12, further comprising a second washing step of: and cleaning the sample treated by the counterstaining step.
The method for staining a specimen according to any one of claims 1 to 13, further comprising:

when the control unit judges that no sample needs to be dyed or receives a first dye liquor recovery instruction sent by a user, recovering the first dye liquor in the dyeing tank to a first dye liquor sealed container for storage, and waiting for next use;

and when the control unit judges that a sample needs to be dyed or receives a first dye liquor feeding instruction sent by a user, the first dye liquor stored in the first dye liquor sealed container is sent to the dyeing tank again so as to recycle the first dye liquor.
The method for staining a specimen according to any one of claims 1 to 14, further comprising:

when the control unit judges that no sample needs to be dyed or receives a first dye solution sealing instruction sent by a user, sealing the first dye solution in the dyeing tank for the next use;

and when the control unit judges that a sample needs to be dyed or receives a first dye solution opening instruction sent by a user, the control unit releases the sealing cover of the dyeing tank so as to recycle the first dye solution.
The method for staining a sample according to claim 14 or 15, wherein the manner in which the control unit determines that no sample needs to be subjected to the staining step comprises: no sample and no sample waiting to be fed into the staining bath, and/or the idle time of the staining bath exceeds a set value.
The specimen staining method according to any one of claims 1 to 16, wherein the first staining solution is drained when the control unit determines that the first staining solution meets a set draining requirement or receives a first staining solution draining instruction from a user.
The method for staining a specimen according to claim 17, wherein the discharge requirement comprises a number of staining times per ml of the first staining solution of 2 or more and/or a time of use of the first staining solution of 2 or more days.
The method for staining a specimen according to any one of claims 1 to 18, further comprising:

when the control unit judges that no sample needs to be subjected to the re-dyeing step or receives a second dye liquor recovery instruction sent by a user, recovering the second dye liquor in the re-dyeing tank to a second dye liquor recovery container for storage, and waiting for next use;

and after the control unit judges that a sample needs to be subjected to the re-dyeing step or receives a second dye liquor feeding instruction sent by a user, re-sending the second dye liquor stored in the second dye liquor recovery container to a re-dyeing tank so as to recycle the second dye liquor.
The method for staining a specimen according to any one of claims 1 to 19, further comprising:

when the control unit judges that no sample needs to be subjected to the re-dyeing step or receives a second dye liquor sealing instruction sent by a user, sealing and covering the second dye liquor in the re-dyeing tank for next use;

and when the control unit judges that a sample needs to be subjected to the secondary dyeing step or receives a second dyeing liquid opening instruction sent by a user, the control unit releases the sealing cover of the secondary dyeing tank so as to recycle the second dyeing liquid.
The method for staining a sample according to claim 19 or 20, wherein the control unit determines that no sample needs to be subjected to the counterstaining step by: the re-dyeing tank and the dyeing tank have no sample and no sample to be sent, and/or the idle time of the re-dyeing tank exceeds a set value.
The specimen staining method according to any one of claims 1 to 21, wherein the second staining solution is drained after the control unit determines that the second staining solution meets a set draining requirement or receives a second staining solution draining instruction from a user.
The method for staining a sample according to claim 4 or 5, further comprising:

when the control unit judges that no sample needs to be subjected to the cleaning step before counterstaining or receives a sealing cover cleaning liquid instruction sent by a user, sealing the cleaning liquid in the cleaning tank, and waiting for the next use;

and when the control unit judges that a sample needs to be subjected to the cleaning step before the counterstaining or receives a cleaning liquid opening instruction sent by a user, the control unit removes the cover of the cleaning tank so as to recycle the cleaning liquid.
The method of staining a specimen according to claim 2, further comprising:

when the control unit judges that no sample needs to be subjected to the dyeing promotion step or receives a cover-sealing dyeing promotion liquid instruction sent by a user, the buffer solution in the dyeing promotion groove is covered for the next use;

and when the control unit judges that the sample needs to be subjected to the dyeing promotion step or receives a dyeing promotion liquid opening instruction sent by a user, the control unit releases the sealing cover of the dyeing promotion groove so as to recycle the buffer solution.
The utility model provides a smear preparation equipment, its characterized in that, includes storage tank, transport mechanism and the control unit, the storage tank includes:

a staining bath for storing a first staining solution, the first staining solution being a mixture of a first biological dye and a second biological dye, the first biological dye being capable of staining eosinophilic substances in the sample, the second biological dye being capable of staining basophilic substances in the sample, the staining bath having a first opening through which a slide coated with the sample can pass;

a counterstaining bath for holding a second staining solution different from the first staining solution, the second staining solution being a mixture of a third biological dye and a buffer, the third biological dye being capable of staining an alkalophilic substance or an alkalophilic substance in the specimen, the counterstaining bath having a second opening through which the slide can pass;

the conveying mechanism is used for picking up the slide and driving the slide to move;

the control unit outputs a control signal to control the conveying mechanism to drive the sample to be soaked in the dyeing tank for dyeing firstly, and then soaked in the dyeing tank for dyeing again.
The smear preparation apparatus of claim 25, further comprising a first dye liquor path control system and a first dye liquor sealed container, wherein the first dye liquor path control system is respectively communicated with the first dye liquor sealed container and the dyeing bath for recycling the corresponding first dye liquor in the dyeing bath to the first dye liquor sealed container and discharging the first dye liquor in the first dye liquor sealed container to the dyeing bath again.
The smear preparation apparatus of claim 26, wherein the first dye solution path control system comprises a first dye solution recovery system and a first dye solution inlet system, the first dye solution recovery system is respectively communicated with the first dye solution sealed container and the dye vat for recovering the corresponding first dye solution in the dye vat to the first dye solution sealed container; and the first dye liquor inlet system is respectively communicated with the first dye liquor sealed container and the dyeing tank and is used for discharging the first dye liquor in the first dye liquor sealed container into the dyeing tank again.
The smear preparation apparatus of claim 27 wherein the first stain recovery system comprises a first stain recovery line in communication with the stain bath and the first stain containment vessel, respectively, and a first stain recovery pressure source for drawing the first stain in the stain bath into the first stain containment vessel;

the first dye liquor inlet system comprises a first dye liquor inlet pipeline and a first dye liquor inlet pressure source, the first dye liquor inlet pipeline is respectively communicated with the dyeing tank and the first dye liquor sealed container, and the first dye liquor in the first dye liquor sealed container is discharged into the dyeing tank by the first dye liquor inlet pressure source.
The smear preparation apparatus of claim 28 wherein the first stain recovery conduit is the same conduit as the first stain inlet conduit and/or the first stain inlet pressure source and the first stain recovery pressure source are the same pressure source.
The smear preparation apparatus of any one of claims 26-29, wherein the first dye solution path control system recovers the corresponding first dye solution in the dye bath to the first dye solution sealed container after the control unit determines that no sample needs to be performed for dyeing work or receives a first dye solution recovery instruction from a user; and when the control unit judges that a sample needs to be dyed or receives a first dye liquor inlet instruction sent by a user, the first dye liquor path control system discharges the first dye liquor in the first dye liquor sealed container into the dyeing tank again.
The smear preparation apparatus of claim 30 wherein the first stain sealed container is a reagent bottle disposed inside the smear preparation apparatus.
The smear preparation apparatus of any one of claims 25-31, further comprising a first dye liquor cover, wherein the first dye liquor cover shields a corresponding opening on the dye vat and reopens the dye vat when needed after the control unit determines that no sample needs to be stained or receives a user instruction to cover the first dye liquor.
The smear preparation apparatus of any one of claims 30-32 wherein the means by which the control unit determines that no sample requires staining comprises: the staining bath has no sample and no sample to be stained is fed in, and/or the staining bath idle time exceeds a set value.
The smear preparation apparatus of claim 26, further comprising a first dye liquor draining system, the first dye liquor draining system being in communication with the dye vat or the first dye liquor sealed container, the first dye liquor draining system draining the first dye liquor in the dye vat or the first dye liquor sealed container when the control unit determines that the first dye liquor meets the draining requirement or receives a first dye liquor draining instruction from a user.
The smear preparation apparatus of claim 34 wherein the discharge requirements include: dyeing is carried out at least 2 times per milliliter of the first dye liquor and/or the first dye liquor is used for more than 2 days.
The smear preparation apparatus of claim 25, further comprising a second stain path control system and a second stain recovery container, the second stain path control system communicating with the second stain sealed container and the re-staining bath for recovering the corresponding second stain in the re-staining bath to the second stain recovery container and re-discharging the second stain in the second stain recovery container to the re-staining bath.
The smear preparation apparatus of claim 36, wherein the second stain path control system comprises a second stain recovery system and a second stain inlet system, the second stain recovery system being in communication with the second stain recovery container and the re-staining bath for recovering the corresponding second stain in the re-staining bath to the second stain recovery container; and the second dye liquor inlet system is communicated with the second dye liquor recovery container and the re-dyeing tank and is used for discharging the second dye liquor in the second dye liquor recovery container into the re-dyeing tank again.
The smear preparation apparatus of claim 37 wherein the second stain recovery system includes a second stain recovery line in communication with the counterstain bath and a second stain recovery vessel, respectively, and a second stain recovery pressure source for drawing the second stain in the counterstain bath into the second stain recovery vessel;

the second dye liquor inlet system comprises a second dye liquor inlet pipeline and a second dye liquor inlet pressure source, the second dye liquor inlet pipeline is respectively communicated with the secondary dyeing tank and the second dye liquor recovery container, and the second dye liquor in the second dye liquor recovery container is discharged into the secondary dyeing tank by the second dye liquor inlet pressure source.
The smear preparation apparatus of claim 38, wherein the second stain recovery conduit is the same conduit as the second stain inlet conduit and/or the second stain inlet pressure source and the second stain recovery pressure source are the same pressure source.
The smear preparation apparatus of any one of claims 36-39, wherein the second dye liquor path control system recovers the corresponding second dye liquor in the re-dyeing bath to a second dye liquor recovery container after the control unit determines that no sample needs to be re-dyed or receives a second dye liquor recovery instruction from the user;

and when the control unit judges that a sample needs to be subjected to re-dyeing work or receives a second dye liquor feeding instruction sent by a user, the second dye liquor path control system discharges the second dye liquor in the second dye liquor recovery container into a re-dyeing tank again.
The smear preparation apparatus of any one of claims 25-40, further comprising a second dye liquor cover, wherein the second dye liquor cover is controlled to shield the re-dyeing bath after the control unit determines that no sample needs to be re-dyed or receives a second dye liquor instruction for covering the re-dyeing bath from the user; and when the control unit judges that a sample needs to be subjected to the re-dyeing work or receives a second dye liquor opening instruction sent by a user, controlling the second dye liquor cover to open the re-dyeing tank.
The smear preparation apparatus of any one of claims 25-41, wherein the receiving bath further comprises a stain promotion bath for holding a buffer, the stain promotion bath having a third opening through which the slide passes; the control unit outputs a control signal to control the conveying mechanism to drive the sample to sequentially move into the dyeing tank for soaking and dyeing, the dye promotion tank for dyeing promotion and the dyeing tank for dyeing reduplication.
The smear preparation apparatus of claim 42, wherein the reservoir further comprises a cleaning bath for holding a cleaning liquid, the cleaning bath having a fourth opening through which the slide passes; after the sample is dyed in the dyeing promotion tank and before the sample is dyed in the dyeing promotion tank again, the control unit outputs a control signal to control the conveying mechanism to drive the sample to move into the cleaning tank for cleaning.
The smear preparation apparatus of claim 43, wherein the staining bath, the stain promoting bath, the washing bath, and the counterstaining bath are arranged in a queue in sequence.
The smear preparation apparatus of any one of claims 25-41, wherein the reservoir further comprises a cleaning bath for holding a cleaning liquid, the cleaning bath having a fourth opening through which the slide passes; the control unit outputs a control signal to control the conveying mechanism to drive the sample to sequentially move to the dyeing tank for soaking and dyeing, the cleaning tank for cleaning and the dyeing tank for dyeing again.
The smear preparation apparatus of claim 43 or 45, further comprising a cleaning liquid cover, wherein the cleaning liquid cover is controlled to shield a corresponding opening of the cleaning bath and reopen the cleaning bath when needed after the control unit determines that no sample needs to be subjected to pre-counterstaining cleaning work or receives a cover-sealing cleaning liquid command from a user.
The smear preparation apparatus of claim 42, further comprising a stain promotion liquid cover, wherein after the control unit judges that no sample needs to be subjected to stain promotion work or receives a cover-sealing stain promotion liquid instruction from a user, the stain promotion liquid cover is controlled to shield a corresponding opening on the stain promotion tank and reopen the stain promotion tank when needed.
The smear preparation apparatus of any one of claims 25-47, further comprising a mounting block and a first drive assembly, wherein the receiving slot is mounted on the mounting block, the mounting block is in driving connection with the first drive assembly, and the receiving slot on the mounting block is moved by the first drive assembly.
The smear preparation apparatus of claim 25, further comprising an integral cover for covering the corresponding opening in the receiving slot when the receiving slot is not in use.
The smear preparation apparatus of claim 49, further comprising a mounting base and a first drive assembly, wherein the receiving slot is mounted on the mounting base, and either of the integral cover and the mounting base is drivingly connected to the first drive assembly for moving the integral cover and the receiving slot on the mounting base relative to each other to shield the corresponding opening on each receiving slot under the drive of the first drive assembly.
The smear preparation apparatus of any one of claims 25-50, further comprising an inlet system, wherein a liquid outlet of the inlet system is in communication with the receiving chamber for injecting a corresponding liquid into the receiving chamber.
A staining solution combination for sample staining, comprising a first staining solution and a second staining solution, wherein the first staining solution is a mixture of a first biological dye and a second biological dye, the first biological dye can stain acidophilic substances in a sample, the second biological dye can stain alkalophilic substances in the sample, the second staining solution is a mixture of a third biological dye and a buffer solution, and the third biological dye can stain alkalophilic substances or acidophilic substances in the sample.
The staining solution combination of claim 52, wherein the first biological dye is an eosin dye, the second biological dye is a methylene blue dye, and the third biological dye is an azure dye.
The dyeing bath combination according to claim 53, wherein the first bath is a Reed-Giemsa bath, the second bath is a mixture of Giemsa bath and buffer, or the second bath is a mixture of Liu's B bath and buffer.
The dyeing bath combination according to any one of claims 52 to 54, wherein the volume ratio a of Giemsa or Liu's B bath to buffer in the second bath is 1/30 ≦ a ≦ 1/10.
The staining solution combination of claim 52, wherein the second biological dye and the third biological dye are the same biological dye.