CN105300772A - Wright-Giemsa compound staining solution and preparation method thereof - Google Patents
Wright-Giemsa compound staining solution and preparation method thereof Download PDFInfo
- Publication number
- CN105300772A CN105300772A CN201510640377.8A CN201510640377A CN105300772A CN 105300772 A CN105300772 A CN 105300772A CN 201510640377 A CN201510640377 A CN 201510640377A CN 105300772 A CN105300772 A CN 105300772A
- Authority
- CN
- China
- Prior art keywords
- parts
- volume
- powder
- methyl alcohol
- jim
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a Wright-Giemsa compound staining solution. The Wright-Giemsa compound staining solution is prepared from the following raw materials according to the following proportion: 15 to 17 parts of Wright staining powder in weight, 3 to 4 parts of Giemsa staining powder in weight, 33 to 35 parts of glycerinum in volume and 5000 parts of methanol in volume. The invention further provides a preparation method and application of the compound staining solution. The Wright-Giemsa compound staining solution has the advantages of high staining speed and good staining effect on human sources and animal cells, simple formula, low preparation cost and simplicity in operation during staining and is favorable for popularization.
Description
Technical field
The present invention relates to a kind of biological reagent, particularly a kind of Rui Shi-Jim Sa compound stain solution and its production and use.
Background technology
Cell dyeing is technological means the most basic in histology, embryology, pathology, research and teaching, and good Color is the morphological observation most important condition accurately.Rui Shi (Wright) dye liquor, Jim Sa (Giemsa) dye liquor are the normal dyeing liquid of cytoscopy, but it is all not ideal enough to be used alone effect, wherein, Wright's stain is better to cytoplasmic components dyeing, but poor to nuclear coloring effect; Giemsa stain is better painted to nucleus, but to cytoplasm and cell membrane and the dyeing of neutrophil granule wherein not good enough.
In order to the shortcoming overcoming Wright's stain, Giemsa stain dyeing exists, the compound stain solution formula of software engineering researchers invent simultaneously containing Rui Shi dyestuff and Jim Sa dyestuff, and achieve certain application in clinical practice.But the Rui Shi-Jim Sa compound stain solution still existing defects of report at present, or dyeing time is long, Color is poor; Compound stain solution formula is complicated, and cost is high.Cost of development is cheap, Color good, Rui Shi easy to use-Jim Sa compound stain solution, has important practical significance to clinical, research and teaching.
Summary of the invention
The object of the present invention is to provide a kind of with low cost, Color good, Rui Shi easy to use-Jim Sa compound stain solution.
The invention provides a kind of Rui Shi-Jim Sa compound stain solution, it is prepared from by the raw material of following proportioning:
Rui Shi contaminates powder 15-17 weight portion, Jim Sa dye powder 3-4 weight portion, glycerine 33-35 parts by volume, methyl alcohol 5000 parts by volume.
Wherein, described weight portion/parts by volume is corresponding with g/ml.
Wherein, described methyl alcohol is pure for analyzing.
Wherein, it is prepared from by the raw material of following proportioning:
Rui Shi contaminates powder 15 weight portion, Jim Sa dye powder 3 weight portion, glycerine 33 parts by volume, methyl alcohol 5000 parts by volume.
Wherein, it is prepared from by following method:
A, take the raw material of each proportioning;
B, Rui Shi contaminated powder, Jim Sa dye powder, glycerine mixing, grind 2 hours, then place 12-16 hour for 37 DEG C, grind 15-20 minute;
C, add 500 parts by volume methyl alcohol, continue grinding and place 15-20 minute after 15 minutes, take out upper strata dye liquor;
D, repetition step c many times are 5000 parts by volume to the total consumption of methyl alcohol;
At room temperature, sealing is kept in Dark Place 5 days for e, the upper strata dye liquor that step c, d obtained, each jolting sooner or later 2 minutes every day; Seal the first quarter moon that keeps in Dark Place again, after filtration.
The invention provides a kind of preparation method of compound stain solution, it comprises the steps:
A, take the raw material of following proportioning: Rui Shi contaminates powder 15-17 weight portion, Jim Sa dye powder 3-4 weight portion, glycerine 33-35 parts by volume, methyl alcohol 5000 parts by volume;
B, Rui Shi contaminated powder, Jim Sa dye powder, glycerine mixing, grind 2 hours, then place 12-16 hour for 37 DEG C, grind 15-20 minute;
C, add 500 parts by volume methyl alcohol, continue grinding and place 15-20 minute after 15 minutes, take out upper strata dye liquor;
D, repetition step c many times are 5000 parts by volume to the total consumption of methyl alcohol;
At room temperature, sealing is kept in Dark Place 5 days for e, the upper strata dye liquor that step c, d obtained, each jolting sooner or later 2 minutes every day; Seal the first quarter moon that keeps in Dark Place again, after filtration.
Wherein, in step (a), described methyl alcohol is pure for analyzing.
Wherein, in step (a), described raw material is that Rui Shi contaminates powder 15 weight portion, Jim Sa dye powder 3 weight portion, glycerine 33 parts by volume, methyl alcohol 5000 parts by volume.
Present invention also offers the purposes of above-mentioned compound stain solution in cell dyeing.
Wherein, described cell refers to human archeocyte and/or zooblast.
Wherein, described cell dyeing refers to the dyeing to blood film and bone marrow smear.
Rui Shi of the present invention-Jim Sa compound stain solution, with Rui Shi dyestuff and Jim Sa dyestuff for primary raw material is formulated by grinding, can be effectively applied to cell dyeing, especially be applied to blood and marrow cell coating plate.Use dye liquor of the present invention can present cell dyeing effect clearly, make the kytoplasm of cell, particle, karyon etc. all obtain satisfied Color, the cellular resolution dyed is good; And compound stain solution dyeing of the present invention is quick, formula for dye liquor is simple, preparation cost is low, simple to operate during dyeing, is beneficial to popularization.
Mostly Rui Shi-Jim Sa the compound stain solution of current report is to carry out staining examine to human archeocyte, does not consider zooblast Color.Inventor finds in research process, and commercially available Rui Shi-Giemsa staining liquid (buying from Zhuhai shellfish rope) is although effectively dye to human archeocyte, poor to the painted difficulty of zooblast, Color.
Dye liquor of the present invention not only effectively can dye to human archeocyte, and good to zooblast Color, meets the dyeing demand to marrow and blood smear in animal experiment research, effectively can be applied to clinical, teaching and animal experiment inspection.
Obviously, according to foregoing of the present invention, according to ordinary technical knowledge and the customary means of this area, not departing under the present invention's above-mentioned basic fundamental thought prerequisite, the amendment of other various ways, replacement or change can also be made.
The embodiment of form by the following examples, is described in further detail foregoing of the present invention again.But this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following example.All technology realized based on foregoing of the present invention all belong to scope of the present invention.
Accompanying drawing explanation
The mature index (RA) of Fig. 1 compound stain solution of the present invention
Fig. 2 contrasts the rat marrow smear (1000 times of visuals field) of dye liquor.
Fig. 3 contrasts the dog bone marrow smear (400 times of visuals field) of dye liquor.
The rat marrow smear of Fig. 4 dye liquor of the present invention, normal bone marrow resembles, and 4A is 400 times of visuals field, and 4B is 1000 times of visuals field.
The rat marrow smear of Fig. 5 dye liquor of the present invention, lymthoma bone marrow infiltration (1000 times of visuals field).
Embodiment
Be described further with embodiment below, but the present invention is not limited to these embodiments.
The present invention's reagent used, instrument are as follows:
Rui Shi contaminates powder, Jim Sa dye powder: RS-Sigma;
Methyl alcohol (AR level), glycerine: Chengdu Ke Long chemical reagent factory;
Microscope: Olympus microscope (OLYMPUSBX51).
The preparation of embodiment 1 compound stain solution of the present invention
1, formula for dye liquor
Rui Shi contaminates powder 15g, Jim Sa dye powder 3g/ glycerine 33mL/ methyl alcohol 5000mL.
2, preparation method
Get Rui Shi and contaminate powder 15g, Jim Sa dye powder 3g, glycerine 33mL, puts in clean mortar and grinds 37 DEG C of placements 15.5 hours of spending the night after 2 hours;
After taking-up, grind after 15-20 minute, add 500mL methyl alcohol, grinding places 15-20 minute in a moment, sucking-off upper strata dye liquor;
In mortar, add 500mL methyl alcohol again, continue grinding and place 15-20 minute after 15 minutes, sucking-off upper strata dye liquor; Continuous several times like this shares methyl alcohol (analyzing pure) 5000ml;
The all upper stratas dye liquor obtained is collected in sealing in Brown Glass Brown glass bottles and jars only to preserve, room temperature is placed, each jolting sooner or later 2 minutes every day, totally 5 days, then deposits first quarter moon, can use, can preserve for a long time after preparation after filtration.
3, Testing index
The mature index (RA) of Rui Shi of the present invention-Jim Sa compound stain solution is (A640 ± 10nm/A520 ± 5nm)=1.4 ± 0.1.
The preparation of embodiment 2 compound stain solution of the present invention
1, formula for dye liquor
Rui Shi contaminates powder 17g, Jim Sa dye powder 4g, glycerine 35mL, methyl alcohol 5000mL.
2, preparation method and Testing index are with embodiment 1.
Below by the mode of test example, beneficial effect of the present invention is described:
Test example 1 dye liquor of the present invention is to the dyeing of marrow smear
One, test material
Dye liquor of the present invention: the compound stain solution prepared according to embodiment 1 method;
Contrast dye liquor: commercially available Rui Shi-Giemsa staining liquid (producer: Zhuhai shellfish rope lot number: 415016 terms of validity: 2017-1-29);
Bone marrow smear: rat smear, dog smear.
Two, colouring method
1, I liquid (dye liquor of the present invention or contrast dye liquor) is added: on smear, drip I liquid, cover whole cell membrane for degree with dye liquor, leave standstill 0.5 ~ 1 minute.
2, add II liquid (PBS damping fluid): II liquid dripping equivalent, blowing with ear washing bulb mixes, and dyes.
Three, coloration result
The coloration result of contrast dye liquor to bone marrow smear is shown in Fig. 2-3, and wherein Fig. 2 is the rat marrow smear under 1000 times of visuals field; Fig. 3 is the dog bone marrow smear under 400 times of visuals field.
The coloration result of dye liquor of the present invention to bone marrow smear is shown in Fig. 4-5, and wherein Fig. 4 is the rat marrow smear of normal bone marrow elephant, and Fig. 5 is the rat marrow smear of lymthoma bone marrow infiltration.
From Fig. 2, Fig. 3, contrast dye liquor is to marrow smear staining poor effect: smear cells is by look overall blueing, under causing mirror, identification is difficult, thus affects cell observation, even if the ratio of adjustment dye liquor and phosphate buffer (II liquid) also can not solve; And with cell not easy coloring during the dyeing of contrast dye liquor, lengthen dyeing time and also can not strengthen Color, dyeing course length consuming time, easily cause dyestuff sediment to be attached on smear.
From Fig. 4, Fig. 5, when using dye liquor of the present invention to dye, smear cells can be made at short notice to be subject to look even, it is clear that different colours is differentiated, red blood cell dye pink, and karyocyte caryoplasm is clearly demarcated, nuclei dyeing aubergine, chromatin and karyoplastin clear, thickness degree of tightness can be distinguished, presents cell dyeing effect clearly.And to same smear, contrast dye liquor dyeing 10-20 minute effect is still undesirable, dyes just can reach good Color in 5 minutes with dye liquor of the present invention.
Visible, dye liquor of the present invention is good to the Color of marrow smear, and dye liquor of the present invention can be effective to cell dyeing.
In sum, Rui Shi of the present invention-Jim Sa compound stain solution, quick to the dyeing of people source and zooblast, Color good, and also compound stain solution formula of the present invention is simple, preparation cost is low, and simple to operate during dyeing, be beneficial to popularization.
Claims (10)
1. Rui Shi-Jim Sa compound stain solution, is characterized in that: it is prepared from by the raw material of following proportioning:
Rui Shi contaminates powder 15-17 weight portion, Jim Sa dye powder 3-4 weight portion, glycerine 33-35 parts by volume, methyl alcohol 5000 parts by volume.
2. compound stain solution according to claim 1, is characterized in that: described methyl alcohol is pure for analyzing.
3. compound stain solution according to claim 1 and 2, is characterized in that: it is by following proportioning
Raw material be prepared from:
Rui Shi contaminates powder 15 weight portion, Jim Sa dye powder 3 weight portion, glycerine 33 parts by volume, methyl alcohol 5000 parts by volume.
4. the compound stain solution according to claim 1-3 any one, is characterized in that: it is prepared from by following method:
A, take the raw material of each proportioning;
B, Rui Shi contaminated powder, Jim Sa dye powder, glycerine mixing, grind 2 hours, then place 12-16 hour for 37 DEG C, grind 15-20 minute;
C, add 500 parts by volume methyl alcohol, continue grinding and place 15-20 minute after 15 minutes, take out upper strata dye liquor;
D, repetition step c many times are 5000 parts by volume to the total consumption of methyl alcohol;
At room temperature, sealing is kept in Dark Place 5 days for e, the upper strata dye liquor that step c, d obtained, each jolting sooner or later 2 minutes every day; Seal the first quarter moon that keeps in Dark Place again, after filtration.
5. a preparation method for compound stain solution, is characterized in that: it comprises the steps:
A, take the raw material of following proportioning: Rui Shi contaminates powder 15-17 weight portion, Jim Sa dye powder 3-4 weight portion, glycerine 33-35 parts by volume, methyl alcohol 5000 parts by volume;
B, Rui Shi contaminated powder, Jim Sa dye powder, glycerine mixing, grind 2 hours, then place 12-16 hour for 37 DEG C, grind 15-20 minute;
C, add 500 parts by volume methyl alcohol, continue grinding and place 15-20 minute after 15 minutes, take out upper strata dye liquor;
D, repetition step c many times are 5000 parts by volume to the total consumption of methyl alcohol;
At room temperature, sealing is kept in Dark Place 5 days for e, the upper strata dye liquor that step c, d obtained, each jolting sooner or later 2 minutes every day; Seal the first quarter moon that keeps in Dark Place again, after filtration.
6. preparation method according to claim 5, is characterized in that: in step (a), and described methyl alcohol is pure for analyzing.
7. preparation method according to claim 5, is characterized in that: in step (a), and described raw material is that Rui Shi contaminates powder 15 weight portion, Jim Sa dye powder 3 weight portion, glycerine 33 parts by volume, methyl alcohol 5000 parts by volume.
8. the purposes of the compound stain solution described in claim 1-4 any one in cell dyeing.
9. purposes according to claim 8, is characterized in that: described cell refers to human archeocyte and/or zooblast.
10. purposes according to claim 9, is characterized in that: described cell dyeing refers to the dyeing to blood film and bone marrow smear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510640377.8A CN105300772A (en) | 2015-09-30 | 2015-09-30 | Wright-Giemsa compound staining solution and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510640377.8A CN105300772A (en) | 2015-09-30 | 2015-09-30 | Wright-Giemsa compound staining solution and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105300772A true CN105300772A (en) | 2016-02-03 |
Family
ID=55198286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510640377.8A Pending CN105300772A (en) | 2015-09-30 | 2015-09-30 | Wright-Giemsa compound staining solution and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105300772A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106053167A (en) * | 2016-05-19 | 2016-10-26 | 四川金域医学检验中心有限公司 | Preparation method of marrow fluid smear |
| CN107300495A (en) * | 2017-06-15 | 2017-10-27 | 托克逊县夏乡畜牧业服务中心 | A kind of colouring method of pyriform worm blood film compound stain solution and blood film |
| CN108414327A (en) * | 2017-10-31 | 2018-08-17 | 天津协和华美医学诊断技术有限公司 | A kind of Wright-Giemsa staining reagents and its application method |
| CN108456710A (en) * | 2018-01-19 | 2018-08-28 | 潍坊医学院 | A kind of Brucella method for detecting |
| CN108663252A (en) * | 2018-08-14 | 2018-10-16 | 苏州丰泰医疗用品贸易有限公司 | A kind of method of quick carry out Rui Shi Giemsa stainings |
| CN108676838A (en) * | 2018-04-11 | 2018-10-19 | 马爽 | A kind of cryptococcus capsule stain liquid and its preparation and application |
| CN109971212A (en) * | 2019-03-05 | 2019-07-05 | 桂林优利特医疗电子有限公司 | A kind of Rui Shi-Jim Sa compound stain solution and preparation method thereof |
| CN110907254A (en) * | 2019-11-04 | 2020-03-24 | 成都市第六人民医院 | Wright-Giemsa dyeing reagent and preparation method thereof |
| CN112840195A (en) * | 2019-06-27 | 2021-05-25 | 深圳迈瑞生物医疗电子股份有限公司 | Sample staining method, smear preparation equipment and staining solution combination |
| CN117990471A (en) * | 2024-04-03 | 2024-05-07 | 苏州良辰生物医药科技有限公司 | Cell stain and application thereof in virus TCID50 determination |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1434127A (en) * | 2002-01-22 | 2003-08-06 | 裴芳君 | Staining kit and preparation method, staining method, and use thereof |
| CN1924543A (en) * | 2006-09-02 | 2007-03-07 | 江西特康科技有限公司 | Blood smear leucocyte dye liquor and its preparing process |
| CN201681010U (en) * | 2010-04-13 | 2010-12-22 | 滕文友 | Automation multi-function staining apparatus |
| CN103323313A (en) * | 2012-03-23 | 2013-09-25 | 黄伏生 | Liquid-based cell analyzer staining method of cells exfoliated from serous cavity by using Wright staining method |
| US20140371090A1 (en) * | 2011-12-31 | 2014-12-18 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Method and kit for determining- antibody sensitivity and clone cell strain |
-
2015
- 2015-09-30 CN CN201510640377.8A patent/CN105300772A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1434127A (en) * | 2002-01-22 | 2003-08-06 | 裴芳君 | Staining kit and preparation method, staining method, and use thereof |
| CN1924543A (en) * | 2006-09-02 | 2007-03-07 | 江西特康科技有限公司 | Blood smear leucocyte dye liquor and its preparing process |
| CN201681010U (en) * | 2010-04-13 | 2010-12-22 | 滕文友 | Automation multi-function staining apparatus |
| US20140371090A1 (en) * | 2011-12-31 | 2014-12-18 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Method and kit for determining- antibody sensitivity and clone cell strain |
| CN103323313A (en) * | 2012-03-23 | 2013-09-25 | 黄伏生 | Liquid-based cell analyzer staining method of cells exfoliated from serous cavity by using Wright staining method |
Non-Patent Citations (2)
| Title |
|---|
| 北京雷根生物技术有限公司: "瑞氏-吉姆萨复合染液", 《百度文库》 * |
| 谈以锐 等: "瑞氏姬姆萨染液联合配制的应用", 《临床检验杂志》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106053167A (en) * | 2016-05-19 | 2016-10-26 | 四川金域医学检验中心有限公司 | Preparation method of marrow fluid smear |
| CN107300495A (en) * | 2017-06-15 | 2017-10-27 | 托克逊县夏乡畜牧业服务中心 | A kind of colouring method of pyriform worm blood film compound stain solution and blood film |
| CN108414327A (en) * | 2017-10-31 | 2018-08-17 | 天津协和华美医学诊断技术有限公司 | A kind of Wright-Giemsa staining reagents and its application method |
| CN108456710A (en) * | 2018-01-19 | 2018-08-28 | 潍坊医学院 | A kind of Brucella method for detecting |
| CN108676838B (en) * | 2018-04-11 | 2021-09-24 | 马爽 | Cryptococcus capsular staining solution and preparation and use methods thereof |
| CN108676838A (en) * | 2018-04-11 | 2018-10-19 | 马爽 | A kind of cryptococcus capsule stain liquid and its preparation and application |
| CN108663252A (en) * | 2018-08-14 | 2018-10-16 | 苏州丰泰医疗用品贸易有限公司 | A kind of method of quick carry out Rui Shi Giemsa stainings |
| CN109971212A (en) * | 2019-03-05 | 2019-07-05 | 桂林优利特医疗电子有限公司 | A kind of Rui Shi-Jim Sa compound stain solution and preparation method thereof |
| CN112840195A (en) * | 2019-06-27 | 2021-05-25 | 深圳迈瑞生物医疗电子股份有限公司 | Sample staining method, smear preparation equipment and staining solution combination |
| CN112840195B (en) * | 2019-06-27 | 2024-05-07 | 深圳迈瑞生物医疗电子股份有限公司 | Sample dyeing method, smear preparation equipment and dyeing liquid combination |
| CN110907254A (en) * | 2019-11-04 | 2020-03-24 | 成都市第六人民医院 | Wright-Giemsa dyeing reagent and preparation method thereof |
| CN110907254B (en) * | 2019-11-04 | 2022-10-11 | 成都市第六人民医院 | Wright-Giemsa dyeing reagent and preparation method thereof |
| CN117990471A (en) * | 2024-04-03 | 2024-05-07 | 苏州良辰生物医药科技有限公司 | Cell stain and application thereof in virus TCID50 determination |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105300772A (en) | Wright-Giemsa compound staining solution and preparation method thereof | |
| Agley et al. | Isolation and quantitative immunocytochemical characterization of primary myogenic cells and fibroblasts from human skeletal muscle | |
| CN103827654A (en) | System and kit for preparing a cytological sample for examination | |
| CN105651580A (en) | Hematoxylin-eosin mixed staining solution | |
| CN105842037B (en) | Colouring method that is a kind of while showing mast cell and acidophic cell | |
| CN112213172A (en) | Stable vaginal secretion visible component staining solution and preparation method thereof | |
| Ohno et al. | Calcification process dynamics in coral primary polyps as observed using a calcein incubation method | |
| CN109946278A (en) | Fluorescent dye DAPI carries out cell DNA to dye quantitative screening for cancer and diagnostic method | |
| CN106053167A (en) | Preparation method of marrow fluid smear | |
| CN103776679B (en) | A kind of method for reducing biological sample background fluorescence | |
| Kunz et al. | Multicolor 3D confocal imaging of thick tissue sections | |
| CN104726337A (en) | Method for enriching and purifying cryptosporidium and giardia in alga-containing water, and method for determining content of cryptosporidium and giardia | |
| CN108007754A (en) | A kind of one step decoration method of cast-off cells, dye combinations used and kit | |
| CN110907254B (en) | Wright-Giemsa dyeing reagent and preparation method thereof | |
| CN112798322A (en) | 3D cell microsphere paraffin section method | |
| CN106644632A (en) | Artificial substrate for single-layer cell tiling | |
| Marshall et al. | Staining properties and stability of a standardised Romanowsky stain. | |
| Rahmawati et al. | Utilization of 1% of Methylene Blue in Staining Histopathological Preparations at Anatomic Pathology Laboratory | |
| CN104865377A (en) | Staining kit of micro megakaryocytes of bone marrow smear, and using method of staining kit | |
| KR890004808B1 (en) | Stain for bacterial flagella | |
| CN110079116B (en) | Nuclear pulp synchronous dyeing dye TB-EMB | |
| Villanueva | A modified blood stain, useful in the diagnosis and evaluation of hematologic elements | |
| CN108168984A (en) | A kind of protein PAGE gel electrophoresis rapid dyeing kits and colouring method | |
| Zhang et al. | Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids | |
| CN101825607B (en) | Method for separating and detecting over-sulfated chondroitin sulfate in heparin sodium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160203 |