CN108456710A - A kind of Brucella method for detecting - Google Patents
A kind of Brucella method for detecting Download PDFInfo
- Publication number
- CN108456710A CN108456710A CN201810051701.6A CN201810051701A CN108456710A CN 108456710 A CN108456710 A CN 108456710A CN 201810051701 A CN201810051701 A CN 201810051701A CN 108456710 A CN108456710 A CN 108456710A
- Authority
- CN
- China
- Prior art keywords
- brucella
- blood
- nurse
- dye liquor
- rui shi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/23—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Brucella (G)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides a kind of Brucella method for detecting, take the blood of patient to carry out being separately cultured for Brucella, Brucella is detected using Rui Shi Ji's nurse Sa decoration method after being separately cultured.The Brucella that the present invention is used to detect in blood can be such that result is more clear, as the detection method of diagnosis brucellosis, recall rate higher.
Description
Technical field
The present invention relates to biotechnologies, specifically, being to be related to a kind of Brucella method for detecting.
Background technology
The positive bottle detection bacterium conventional coloring method of blood culture is Gram's staining, and detection Brucella is similarly with leather
Blue Albert'stain Albert.But Brucella is because be gram-Negative bacillus, and since thalline is especially small, so what blood culture came out
Brucella is under Gram-staining process and bad discovery, in order to preferably differentiate Brucella, so with Wright's staining into
Row Bacterial stain.
Invention content
It is an object of the invention to overcome the shortcoming of above-mentioned traditional technology, a kind of Brucella method for detecting is provided,
The recall rate of Brucella can be improved.
The purpose of the present invention is what is reached by following technical measures：A kind of Brucella method for detecting, uses Rui Shi-
Ji's nurse Sa decoration method dyes Brucella and detects.
Further, it takes the blood of patient to carry out being separately cultured for Brucella, Rui Shi-Ji's nurse Sa is used after being separately cultured
Decoration method detects Brucella.
Further, blood film is made after being separately cultured, and is fixed before being dyed to blood film, is prevented cell from dyeing
It falls off in journey.
Further, included the following steps using Rui Shi-Ji's nurse Sa decoration method after fixing,
(1) pencil is marked, and blood film is lain on staining rack, wax crayon scribing line can liquidproof it is excessive.
(2) after blood film spontaneously dries, drop Rui Shi-few drops of Ji's nurse Sa dye liquor (A liquid) covers Blood piece, contaminates 1 minute.
(3) buffer solution of slightly more volumes is added dropwise（B liquid）, it is blown to even, dye 5 minutes with rubber pipette bulb with dye liquor.
(4) slide is slowly shaken, then washing away dye liquor from the side of slide with thin originally flow (not remove dye liquor first
Wash by water again), after Blood piece natural drying (or being blotted with filter paper), you can microscopy.
By adopting the above-described technical solution, compared with prior art, it is an advantage of the invention that：
Being separately cultured for Brucella is carried out with the blood of patient, Rui Shi-Ji's nurse Sa decoration method is separately cultured cloth in identification blood
Family name's bacillus method is brucellosis diagnosis " goldstandard "；
Carrying out the detection of Brucella to blood film with Rui Shi-Ji's nurse Sa decoration method more can intuitively judge brucellosis
It whether there is, be not easy to distinguish relative to Brucella color distinction compared with background is smaller under Gram staining Electronic Speculum, Rui Shi-
The result that Ji's nurse Sa decoration method obtains, which can be more clear, to be observed, and color is distinguished obviously, is readily viewed under Electronic Speculum；
The recall rate for improving brucellosis, the foundation as brucellosis diagnosis.
Present invention will be further explained below with reference to the attached drawings and specific embodiments.
Description of the drawings
Attached drawing 1 is the electron microscopic picture under the Brucella visual field that Zhong Ruishi of the present invention-Ji's nurse Sa decoration method obtains.
Attached drawing 2 is the electron microscopic picture under the Brucella visual field that Gram staining obtains in the present invention.
Specific implementation mode
It is smaller not relative to Brucella color distinction compared with background under Gram staining Electronic Speculum as shown in attached drawing 1-2
It is easy to distinguish, the result that Rui Shi-Ji's nurse Sa decoration method obtains, which can be more clear, to be observed, and Brucella is dyed to royal purple
Color is readily viewed under Electronic Speculum.
It takes the blood of patient to carry out being separately cultured for Brucella, is examined using Rui Shi-Ji's nurse Sa decoration method after being separately cultured
Go out Brucella, blood film is made after being separately cultured, be fixed before being dyed to blood film, prevents cell from being taken off in dyeing course
It falls.
Included the following steps using Rui Shi-Ji's nurse Sa decoration method after fixation,
(1) pencil is marked, and blood film is lain on staining rack, wax crayon scribing line can liquidproof it is excessive.
(2) after blood film spontaneously dries, drop Rui Shi-few drops of Ji's nurse Sa dye liquor (A liquid) covers Blood piece, contaminates 1 minute.
(3) buffer solution of slightly more volumes is added dropwise（B liquid）, it is blown to even, dye 5 minutes with rubber pipette bulb with dye liquor.
(4) slide is slowly shaken, then washing away dye liquor from the side of slide with thin originally flow (not remove dye liquor first
Wash by water again), after Blood piece natural drying (or being blotted with filter paper), you can microscopy.
There are following points for attention when making blood film,
(1) it answers flowing water to rinse when rinsing, dye liquor cannot be first outwelled, to prevent dyestuff calmness on blood film.Washing time cannot mistake
Long, with Anti-fade.As there is dye granule deposition on blood film, methanol can be added dropwise, rinsed immediately after with flowing water.
(2) dyed it is light can redye, then plus dye liquor redye Shi Yingxian and add buffer solution,.Hyperchromasia can be rushed with flowing water
It washes or impregnates, it is also possible to which methanol decolourizes.
(3) Wright's staining | liquid is made of Rui Shi dyestuffs, methanol (AR grades or more) and glycerine, and B liquid is phosphate buffer
(pH6.4~ pH6.8)
It detected Brucella in this way, be clinically the doubtful Brucella of report, clinical patients blood culture report is three days positive
After above, positive bottle reservation is sent Disease Control and Prevention Center to be PCR and is made a definite diagnosis.
Claims (4)
1. a kind of Brucella method for detecting, it is characterised in that：Brucella is dyed using Rui Shi-Ji's nurse Sa decoration method and is examined
Go out.
2. a kind of Brucella method for detecting according to claim 1, it is characterised in that：The blood of patient is taken to carry out Bu Shi
Bacillus is separately cultured, and Brucella is detected using Rui Shi-Ji's nurse Sa decoration method after being separately cultured.
3. a kind of Brucella method for detecting according to claim 2, it is characterised in that：Blood is made after being separately cultured to apply
Piece is fixed before being dyed to blood film, prevents cell from falling off in dyeing course.
4. a kind of Brucella method for detecting according to claim 3, it is characterised in that：Rui Shi-Ji's nurse is used after fixation
Sa decoration method includes the following steps,
(1) pencil is marked, and blood film is lain on staining rack, wax crayon scribing line can liquidproof it is excessive；
(2) after blood film spontaneously dries, drop Rui Shi-few drops of Ji's nurse Sa dye liquor (A liquid) covers Blood piece, contaminates 1 minute；
(3) buffer solution of slightly more volumes is added dropwise（B liquid）, it is blown to even, dye 5 minutes with rubber pipette bulb with dye liquor；
(4) slide is slowly shaken, then washing away dye liquor from the side of slide with thin originally flow (not go first dye liquor to rush again
Water), after Blood piece natural drying (or being blotted with filter paper), you can microscopy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810051701.6A CN108456710A (en) | 2018-01-19 | 2018-01-19 | A kind of Brucella method for detecting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810051701.6A CN108456710A (en) | 2018-01-19 | 2018-01-19 | A kind of Brucella method for detecting |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108456710A true CN108456710A (en) | 2018-08-28 |
Family
ID=63220937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810051701.6A Withdrawn CN108456710A (en) | 2018-01-19 | 2018-01-19 | A kind of Brucella method for detecting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108456710A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300772A (en) * | 2015-09-30 | 2016-02-03 | 成都华西海圻医药科技有限公司 | Wright-Giemsa compound staining solution and preparation method thereof |
| CN107475347A (en) * | 2017-10-12 | 2017-12-15 | 天康生物股份有限公司 | A kind of method of quick analysis Brucella sp strain variation rate |
-
2018
- 2018-01-19 CN CN201810051701.6A patent/CN108456710A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105300772A (en) * | 2015-09-30 | 2016-02-03 | 成都华西海圻医药科技有限公司 | Wright-Giemsa compound staining solution and preparation method thereof |
| CN107475347A (en) * | 2017-10-12 | 2017-12-15 | 天康生物股份有限公司 | A kind of method of quick analysis Brucella sp strain variation rate |
Non-Patent Citations (4)
| Title |
|---|
| MST. MINARA KHATUN等: "Hematological profiles during acute and subacute brucellosis in Sprague-Dawley rats experimentally infected with B. abortus biotype 1.", 《INTERNATIONAL JOURNAL OF HEMATOLOGY RESEARCH》 * |
| 中国疾病预防控制中心: "病原", 《HTTP://WWW.CHINACDC.CN/JKZT/CRB/ZL/BLSJB/ZSTD/200509/T20050908_42824.HTML》 * |
| 徐功立等: "《当代血液病的诊治和实验室检查技术》", 31 July 2001 * |
| 徐雷等: "瑞氏-姬姆萨复合染色法在分泌物涂片中的应用价值", 《中国基层医药》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4336337A (en) | Detection of bacteria | |
| CN107250346B (en) | The manufacturing method of cell-preservation liquid and its utilization and cell-preservation liquid | |
| JP2010520487A (en) | Automatic staining method and apparatus for biological materials | |
| CN106970224B (en) | A kind of kit and its application using CD45 immunofluorescences joint CEP probe identification circulating tumor cells | |
| CN106980018B (en) | A kind of kit and its application using CD45 immunofluorescences joint CEP17 probe identification circulating tumor cells | |
| EP2624753A2 (en) | Diagnostic apparatus and diagnostic method using the same | |
| CN106996976B (en) | CTC protein parting kits based on microflow control technique | |
| CN106970225A (en) | A kind of kit of application CD45 immunofluorescences joint probe identification circulating tumor cells of CEP 8 and its application | |
| CN113945440A (en) | Papanicolaou staining kit, preparation method thereof and staining method | |
| CN102260731A (en) | Enzyme-labeling-liquid-based cytology staining kit for screening bladder cancer | |
| CN104711315A (en) | Capsule staining method for streptococcus pneumoniae | |
| CN111004838A (en) | Application of bone marrow smear fluorescence in situ hybridization technology in multiple myeloma | |
| CN108456710A (en) | A kind of Brucella method for detecting | |
| CN108441477A (en) | A method of capture cancer of pancreas circulating tumor cell is enriched with based on CSV antibody | |
| CN108319815A (en) | A kind of method and its system virtually dyed for cell | |
| CN205067292U (en) | Circulation tumor cells detect reagent box | |
| CN107475356A (en) | Osteosarcoma circulating tumor cell identification kit | |
| CN111575239A (en) | Method and device for enriching circulating tumor cells | |
| CN108195653A (en) | One kind redyes staining kit and preparation and application | |
| WO2017096714A1 (en) | Circulating tumor cell typing identification kit | |
| CN109917132A (en) | For the primer pair of 18 genotype of HPV 16 and HPV, dual lateral flow chromatograph test strip and detection method | |
| CN106868104A (en) | Lung cancer circulating tumor cell Classification Identification kit | |
| CN114457139A (en) | Cell detection method and kit | |
| JP2001149099A (en) | Preparation of specimen of nucleus-containing cell for analysis of chromosome abnormality | |
| CN113804880A (en) | Combined detection kit and detection method for measles, mumps and rubella virus IgM antibody |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180828 |