CN108663252A - A kind of method of quick carry out Rui Shi Giemsa stainings - Google Patents
A kind of method of quick carry out Rui Shi Giemsa stainings Download PDFInfo
- Publication number
- CN108663252A CN108663252A CN201810921425.4A CN201810921425A CN108663252A CN 108663252 A CN108663252 A CN 108663252A CN 201810921425 A CN201810921425 A CN 201810921425A CN 108663252 A CN108663252 A CN 108663252A
- Authority
- CN
- China
- Prior art keywords
- rui shi
- liquid
- carry out
- stainings
- giemsa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002738 Giemsa staining Methods 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 37
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000004043 dyeing Methods 0.000 claims abstract description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000000975 dye Substances 0.000 claims abstract description 10
- 235000011187 glycerol Nutrition 0.000 claims abstract description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 4
- 229910000397 disodium phosphate Inorganic materials 0.000 claims abstract description 4
- 235000019800 disodium phosphate Nutrition 0.000 claims abstract description 4
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000001488 sodium phosphate Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000010186 staining Methods 0.000 claims description 3
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 2
- 238000007605 air drying Methods 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 2
- YOWZJZJLXUQHGF-UHFFFAOYSA-M [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;dimethyl-[7-(methylamino)phenothiazin-3-ylidene]azanium;dichloride Chemical compound [Cl-].[Cl-].C1=CC(=[N+](C)C)C=C2SC3=CC(NC)=CC=C3N=C21.C1=CC(=[N+](C)C)C=C2SC3=CC(N(C)C)=CC=C3N=C21 YOWZJZJLXUQHGF-UHFFFAOYSA-M 0.000 abstract description 3
- 238000000386 microscopy Methods 0.000 abstract description 3
- 239000002671 adjuvant Substances 0.000 abstract description 2
- 238000003759 clinical diagnosis Methods 0.000 abstract description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 4
- 229960000907 methylthioninium chloride Drugs 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000008187 granular material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- NALREUIWICQLPS-UHFFFAOYSA-N 7-imino-n,n-dimethylphenothiazin-3-amine;hydrochloride Chemical compound [Cl-].C1=C(N)C=C2SC3=CC(=[N+](C)C)C=CC3=NC2=C1 NALREUIWICQLPS-UHFFFAOYSA-N 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 244000178870 Lavandula angustifolia Species 0.000 description 1
- 235000010663 Lavandula angustifolia Nutrition 0.000 description 1
- SJEYSFABYSGQBG-UHFFFAOYSA-M Patent blue Chemical compound [Na+].C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=CC=1)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1 SJEYSFABYSGQBG-UHFFFAOYSA-M 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000980 acid dye Substances 0.000 description 1
- 239000000981 basic dye Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000001102 lavandula vera Substances 0.000 description 1
- 235000018219 lavender Nutrition 0.000 description 1
- 239000012263 liquid product Substances 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000001665 trituration Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of method of quick carry out Rui Shi Giemsa stainings of Rui Shi Jim Sa technical fields, Rui Shi Giemsa staining liquid A liquid：Weigh Rui Shi dyestuffs 10g, Jim Sa dyestuff 1g, azure II 1g, glycerine 30ml, polysorbas20 15ml, methanol 500ml；Rui Shi Giemsa staining liquid B liquid：Anhydrous potassium dihydrogenphosphate 0.3g, Anhydrous Disodium Phosphate 0.2g, distilled water to 1000ml；This method dyeing time is short, and cooperation overflow dyeing machine uses, and can carry out rapid dyeing, microscopy, adjuvant clinical diagnosis in batches.
Description
Technical field
The present invention relates to Rui Shi Jim Sa technical fields, specially a kind of method of quick carry out Rui Shi Giemsa stainings.
Background technology
Wright's staining is the most frequently used at present and simplest colouring method, acid Yihong and alkaline methylene blue in Wright's stain
Mixing becomes Yihong methylene blue of neutrality after chemical action, and oxidized and contain reddish black after being long placed in, this three kinds of dyestuffs are distinguished
With in nucleus and slurry NH3+ and the combinations such as COO-, so that nucleus and endochylema is coloured, due to being neutral dye, and have buffer solution
Adjust acid-base value, so after cell is contaminted, the colors such as Lan Hong are all more moderate, chromatin, endochylema and wherein particle colour developing more
Clear, Giemsa stain is by reddish black, and Yihong composition, dyeing theory and result and Wright Stain are essentially identical；Acidophilic granule
It for alkaline protein, is combined with acid dyes Yihong, dye pink, referred to as acidophilia substance；Nuclear extract and lymphocyte
Endochylema is acidity, with basic dye methylene blue or reddish black combination, contaminates hyacinthine, referred to as basophilla substance；Neutrophil granule is in equal electric shape
State and Yihong and methylene blue are combinable, contaminate lavender, referred to as neutral substance；To take into account the length of the two, compound staining can be used, but
Traditional flow dyeing time is longer, and dyeing about needs 4min to complete, and need to lead to dye liquor A and dye liquor B on slide in dyeing course
Piping and druming mixing is crossed, it is comparatively laborious, for this purpose, a kind of it is proposed that method of quick carry out Rui Shi Giemsa stainings.
Invention content
The purpose of the present invention is to provide a kind of methods of quick carry out Rui Shi Giemsa stainings, to solve above-mentioned background skill
The problem of being proposed in art.
To achieve the above object, the present invention provides the following technical solutions：A kind of side of quick carry out Rui Shi Giemsa stainings
Method：
Rui Shi Giemsa staining liquid A liquid：
Weigh Rui Shi dyestuffs 10g,
Jim Sa dyestuff 1g,
Azure II 1g,
Glycerine 30ml,
Tween-20 15ml,
Methanol 500ml；
Rui Shi Giemsa staining liquid B liquid：
Anhydrous potassium dihydrogenphosphate 0.3g,
Anhydrous Disodium Phosphate 0.2g,
Distilled water is to 1000ml.
Compared with prior art, the beneficial effects of the invention are as follows：This method dyeing time is short, and cooperation overflow dyeing machine uses, can
To carry out rapid dyeing, microscopy, adjuvant clinical diagnosis in batches.
Specific implementation mode
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment：
A kind of method of quick carry out Rui Shi Giemsa stainings, which includes such as
Lower step：
Rui Shi Giemsa staining liquid A liquid：
A：Weigh Rui Shi dyestuffs 10g, Jim Sa dyestuff 1g, azure II 1g, glycerine 30ml, polysorbas20 15ml and methanol
500ml；
B：After weighing each Rui Shi dyestuffs, Jim Sa dyestuff and azure II number, it is put into mortar and glycerine and tween is added, grinding
1~2h is gradually added methanol dissolving, stands a moment, supernatant liquid is transferred in brown bottle, then adds methanol trituration after finely ground,
Then plus 2 times of filters it is repeated several times, until mortar inner dye and methanol use until exhausted, shakes up, seal bottleneck, when use first filters,
The methanol dilution of liquid product；
Rui Shi Giemsa staining liquid B liquid：
C：Anhydrous potassium dihydrogenphosphate 0.3g and Anhydrous Disodium Phosphate 0.2g are weighed, distilled water is added to make its whole to 1000ml
Dissolving adjusts pH to 6.4~6.8.
Wherein, the sample need to uniformly be applied to slide, and slide needs wiped clean before use；On the slide
Sample needs natural air drying；Sample, and Rui Shi Giemsa staining liquid A liquid pair need to be completely covered in the Rui Shi Giemsa stainings liquid A liquid
Sample contaminates 30 seconds, and sample need to be completely covered in the Rui Shi-Giemsa staining liquid B liquid, and Rui Shi Giemsa staining liquid B liquid is to sample
Dyeing 60 seconds；The sample of the slide needs to wash after working process, sucks moisture, so that sample reaches drying regime；And
The method of the quick carry out Rui Shi Giemsa stainings includes the following steps：
S1：Sample is spread evenly across on clean slide, is spontaneously dried；
S2：Add Rui Shi Giemsa staining liquid A liquid, cover sample, contaminates 30s, discard A liquid；
S3：Add equivalent Rui Shi Giemsa staining liquid B liquid, contaminates 60s；
S4：Dyeing liquor is discarded, washes, sucks moisture, dry, microscopy.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of method of quick carry out Rui Shi Giemsa stainings, it is characterised in that：
Rui Shi Giemsa staining liquid A liquid：
Weigh Rui Shi dyestuffs 10g,
Jim Sa dyestuff 1g,
Azure II 1g,
Glycerine 30ml,
Tween-20 15ml,
Methanol 500ml；
Rui Shi Giemsa staining liquid B liquid：
Anhydrous potassium dihydrogenphosphate 0.3g,
Anhydrous Disodium Phosphate 0.2g,
Distilled water is to 1000ml.
2. a kind of method of quick carry out Rui Shi Giemsa stainings according to claim 1, it is characterised in that：The sample
It need to be uniformly applied to slide, and slide needs wiped clean before use.
3. a kind of method of quick carry out Rui Shi Giemsa stainings according to claim 1, it is characterised in that：The slide
On sample need natural air drying.
4. a kind of method of quick carry out Rui Shi Giemsa stainings according to claim 1, it is characterised in that：The Rui Shi
Sample need to be completely covered in Giemsa staining liquid A liquid, and Rui Shi Giemsa stainings liquid A liquid contaminates sample 30 seconds, the Rui Shi-Jim
Sa dyeing liquor B liquid need to be completely covered sample, and Rui Shi Giemsa stainings liquid B liquid was to specimen staining 60 seconds.
5. a kind of method of quick carry out Rui Shi Giemsa stainings according to claim 1, it is characterised in that：The slide
Sample need to wash after working process, moisture is sucked, so that sample reaches drying regime.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810921425.4A CN108663252A (en) | 2018-08-14 | 2018-08-14 | A kind of method of quick carry out Rui Shi Giemsa stainings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810921425.4A CN108663252A (en) | 2018-08-14 | 2018-08-14 | A kind of method of quick carry out Rui Shi Giemsa stainings |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108663252A true CN108663252A (en) | 2018-10-16 |
Family
ID=63789677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810921425.4A Pending CN108663252A (en) | 2018-08-14 | 2018-08-14 | A kind of method of quick carry out Rui Shi Giemsa stainings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108663252A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109799123A (en) * | 2018-12-29 | 2019-05-24 | 广州和能生物科技有限公司 | A kind of method of quick carry out Rui Shi Giemsa staining |
| CN109813588A (en) * | 2019-02-19 | 2019-05-28 | 湖北泰康医疗设备有限公司 | A kind of helminth dyeing liquor |
| CN109971212A (en) * | 2019-03-05 | 2019-07-05 | 桂林优利特医疗电子有限公司 | A kind of Rui Shi-Jim Sa compound stain solution and preparation method thereof |
| CN110631882A (en) * | 2019-10-22 | 2019-12-31 | 青岛澜澈生物科技有限公司 | Method for online real-time dye liquor mixing in Giemsa reye dyeing |
| CN112840195A (en) * | 2019-06-27 | 2021-05-25 | 深圳迈瑞生物医疗电子股份有限公司 | Sample staining method, smear preparation equipment and staining solution combination |
| CN114295453A (en) * | 2022-01-18 | 2022-04-08 | 黄玉兰 | Giemsa rapae dye and rapid dyeing method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109799123A (en) * | 2018-12-29 | 2019-05-24 | 广州和能生物科技有限公司 | A kind of method of quick carry out Rui Shi Giemsa staining |
| CN109813588A (en) * | 2019-02-19 | 2019-05-28 | 湖北泰康医疗设备有限公司 | A kind of helminth dyeing liquor |
| CN109971212A (en) * | 2019-03-05 | 2019-07-05 | 桂林优利特医疗电子有限公司 | A kind of Rui Shi-Jim Sa compound stain solution and preparation method thereof |
| CN112840195A (en) * | 2019-06-27 | 2021-05-25 | 深圳迈瑞生物医疗电子股份有限公司 | Sample staining method, smear preparation equipment and staining solution combination |
| CN112840195B (en) * | 2019-06-27 | 2024-05-07 | 深圳迈瑞生物医疗电子股份有限公司 | Sample dyeing method, smear preparation equipment and dyeing liquid combination |
| CN110631882A (en) * | 2019-10-22 | 2019-12-31 | 青岛澜澈生物科技有限公司 | Method for online real-time dye liquor mixing in Giemsa reye dyeing |
| CN110631882B (en) * | 2019-10-22 | 2022-01-28 | 青岛澜澈生物科技有限公司 | Method for online real-time dye liquor mixing in Giemsa reye dyeing |
| CN114295453A (en) * | 2022-01-18 | 2022-04-08 | 黄玉兰 | Giemsa rapae dye and rapid dyeing method thereof |
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Application publication date: 20181016 |