CN105167795A - PET/CT macroscopical digital information and pathological microscopic information matching method - Google Patents
PET/CT macroscopical digital information and pathological microscopic information matching method Download PDFInfo
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- CN105167795A CN105167795A CN201510563799.XA CN201510563799A CN105167795A CN 105167795 A CN105167795 A CN 105167795A CN 201510563799 A CN201510563799 A CN 201510563799A CN 105167795 A CN105167795 A CN 105167795A
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Abstract
The invention provides a PET/CT (Positron Emission Tomography/Computed Tomography) macroscopical digital information and pathological microscopic information matching method. A postoperative sample is reduced into an in-vivo state and is soaked into formalin; a cross section is formed between the tracer highest-ingestion layer surface in a PET/CT image and an adjacent anatomic landmark; the sample is cut apart from the middle in the cross section direction, and is soaked into the formalin; HE (Hematoxylin-Eosin) staining pathological sections are manufactured; a paraffin block region of an immune tissue chemical staining region in each HE staining pathological section is determined; the selected paraffin block region is fixed, and the paraffin section cutting is performed; and the parameter of the tracer high-ingestion layer surface in each layer of PET/CT image and the corresponding layer immunohistochemistry expression are analyzed. The PET/CT image information and immunohistochemistry information matching accuracy is improved; a tracer high-ingestion region and a histology region are accurately registered; and after the matching, the accuracy of the PET/CT on the detection of the in-vivo oncobiology information can be verified.
Description
Technical field
The present invention relates to functional image image analysis technology field, particularly relate to the method that a kind of PET/CT macroscopic view digital information is mated with pathology microscopic information.
Background technology
PET/CT (positronemissiontomography/computedtomography) full name is positron emission tomography/x-ray computer tomography instrument, a kind of novel image documentation equipment that PET (functional metabolism video picture) and CT (anatomical structure video picture) two kinds of advanced image technologies are organically combined. it is in human body by the positron radionuclide tracer injection of trace, then special external survey meter (PET) is adopted to detect the distribution situation of each internal organs of these positron radionuclide human bodies, by the physiological metabolism of the major organs of the method display human body of computerized tomograph, apply CT technology accurately to locate for these Nuclear analysis situations simultaneously, make this machine have the advantage of PET and CT simultaneously, respective sharpest edges are given play to.PET/CT has merged the advantage of functional image and anatomical map, has feature that is non-invasive, repeated, quantitative and timely monitor, can ocular and clear in the stereo biological characteristic of body tumor, as the metabolism, propagation, weary oxygen, gene expression etc. of tumor.At present, large slice is only confined to row HE and dyes, cannot the detection of row tumor endogenous markers SABC, therefore it is the difficult point studied at present that the human body how detected by PET/CT carries out mating with histology's pathological diagnosis information in the biological function information of body tumor, limits the development of PET/CT in malignant tumor diagnosis and treatment.
Summary of the invention
Object of the present invention is exactly to solve the problem, a kind of PET/CT macroscopic view method that digital information is mated with pathology microscopic information is provided, the grand design of PET/CT image and histopathology phenotype micro image are carried out post processing and accuracy registration, be conducive to the heterogeneity accurately detected further in tumor, distinguish regions different to radiation-sensitive in tumor.
To achieve these goals, the present invention adopts following technical scheme:
The method that PET/CT macroscopic view digital information is mated with pathology microscopic information, comprises the following steps:
Step one: be reduced to by Postoperative Specimen in body state, then immerses in formalin;
Step 2: form transverse section between tracer uptake highest level and adjacent anatomic landmark in PET/CT image, cuts specimen from centre along this cross-sectional direction, then immerses in formalin;
Step 3: the HE dyeing pathological section making specimen;
Step 4: the wax stone region determining immunohistochemical staining region in every a slice HE dyeing pathological section;
Step 5: wax stone region paraformaldehyde selected in above-mentioned steps four is fixed, then carries out paraffin section, recycle the endogenous markers immunohistochemical staining that this paraffin section carries out detecting, and analyze.
Step 6: the parameter in tracer height picked-up region in every one deck PET/CT image is carried out correlation analysis with the expression of corresponding aspect SABC.
The concrete grammar of described step one is that the tissue intending excision is carried out labelling X under body naturalness, Y, Z, forms specimen after tissue is in vitro, by specimen according in body three-dimensional location, is reduced to specimen in body state.
In described step one, immerse 16-24 hour in formalin.
In described step 2, immerse 8-14 hour in formalin.
In described step one and step 2 formalin used to be concentration of formaldehyde be 35% to 40% formalin.
In described step 2 and step 3, specimen is all cut cutting in specimen plate.
The concrete grammar of described step 3 obtains multiple lamellar specimen for specimen being cut by the continuous parallel lamellar of PET/CT thickness, measures the actual (real) thickness of each lamellar specimen and number consecutively, lamellar specimen is made HE and to dye pathological section.The thickness of each lamellar specimen is corresponding with the thickness of PET/CT, is 4-5mm.
The concrete grammar of described step 4 is determine that HE dyes region corresponding with tracer region of interest in PET/CT image on pathological section under the microscope, will HE cut into slices in region within tumor boundaries and in tracer region of interest be defined as the wax stone region in immunohistochemical staining region.
Paraformaldehyde in described step 5 is the paraformaldehyde of 4%.
Beneficial effect of the present invention:
Invention increases the accuracy of PET/CT image information and SABC information matches, by PET/CT tracer height picked-up region and Immunohistochemical Expression region exact matching, accurate registration is carried out in the region of tracer height picked-up and histological region, can verify after coupling that PET/CT detects the accuracy in body oncobiology information, be conducive to the heterogeneity accurately detected further in tumor, distinguish regions different to radiation-sensitive in tumor, be conducive to selecting different dose irradiation, prerequisite and the basis of individuation radiotherapy, it is the direction of accurate radiotherapy development.
Accompanying drawing explanation
The lung tissue of Fig. 1 (a) for excising in the embodiment of the present invention, lung tissue is reduced to the picture in body state by Fig. 1 (b);
Fig. 2 (a) is for incision aspect is at the schematic diagram of three-dimensional, and Fig. 2 (b) cuts picture for the maximum aspect of tracer uptake, and Fig. 2 (c) be the maximum aspect incision picture of the tracer uptake of band scale;
Fig. 3 (a) is respectively CT window image, mediastinum window image, PET/CT fusion image, the PET image of tracer height picked-up region typical case's aspect, and Fig. 3 (b) is the tissue slice corresponding with this aspect;
Fig. 4 (a) is all Serial tissue sections, and Fig. 4 (b) is the histopathologic slide made continuously by PET/CT thickness;
Fig. 5 (a) is the region of intralesional PET/CT tracer height picked-up, b () HE colored graph picture absorbs corresponding region, region with tracer height, Fig. 5 (c) is the selected zone planning to implement the block sections that SABC detects that the region of dyeing according to tumor boundaries and HE is determined.
Detailed description of the invention
Below in conjunction with accompanying drawing and embodiment, the invention will be further described, is described in the present embodiment for lung tissue.In order to by PET/CT tracer height picked-up region and Immunohistochemical Expression region exact matching, we have invented following methods.
Embodiment one
The method that PET/CT macroscopic view digital information is mated with pathology microscopic information, comprises the following steps:
Step one: as shown in Fig. 1 (a) He Fig. 1 (b), is reduced to Postoperative Specimen in body state, then to immerse in formalin 24 hours; Concrete grammar is in art in thoracic surgery implementation process, and the lung tissue intending excision is carried out labelling X under body naturalness, Y, Z, and lung tissue forms specimen in vitro afterwards, by specimen according in body three-dimensional location, specimen is reduced in body state;
Step 2: as shown in Fig. 2 (a)-Fig. 2 (c), read preoperative PET/CT image again, forming transverse section in PET/CT image between tracer uptake highest level and knuckle (is lung inner disease foci in the present embodiment, so selection is knuckle; As being intracerebral lesion scanning, then select the blood vessel that shows of specimen), specimen is cut from centre along this cross-sectional direction cutting in specimen plate, then to immerse in formalin 14 hours; As Fig. 3 (a) and Fig. 3 (b) is depicted as pathology aspect corresponding to PET/CT;
Step 3: shown in Fig. 4 (a) He Fig. 4 (b), makes the HE dyeing pathological section of specimen; Concrete grammar, for specimen is obtained multiple lamellar specimen cutting specimen plate cuts by the continuous parallel lamellar of PET/CT thickness, measures the actual (real) thickness of each lamellar specimen and number consecutively, by lamellar specimen conveniently pathological process make HE and to dye pathological section; The thickness of each lamellar specimen is 5mm, corresponding with the thickness of PET/CT;
Step 4: the wax stone region determining immunohistochemical staining region in HE dyeing pathological section under the microscope; As shown in Fig. 5 (a) He Fig. 5 (b), concrete grammar is determine region corresponding with PET/CT tracer region of interest on HE dyeing pathological section, region in being cut into slices by HE within tumor boundaries and in tracer region of interest is defined as the wax stone region in immunohistochemical staining region, as shown in Fig. 5 (c);
Step 5: each aspect paraformaldehyde in wax stone region selected in described step 4 is fixed, then carries out paraffin section, recycle this paraffin section and carry out immunohistochemical staining and the analysis of weary oxygen label;
Step 6: the parameter in tracer height picked-up region in every one deck PET/CT image is carried out correlation analysis with the expression of corresponding aspect SABC.
In described step one and step 2 formalin used to be concentration of formaldehyde be 37% formalin.
Paraformaldehyde in described step 5 is the paraformaldehyde of 4% of stamen bio tech ltd, Shanghai hundred.
Embodiment two
The method that PET/CT macroscopic view digital information is mated with pathology microscopic information, comprises the following steps:
Step one: as shown in Fig. 1 (a) He Fig. 1 (b), is reduced to Postoperative Specimen in body state, then to immerse in formalin 16 hours; Concrete grammar is in art in thoracic surgery implementation process, and the lung tissue intending excision is carried out labelling X under body naturalness, Y, Z, and lung tissue forms specimen in vitro afterwards, by specimen according in body three-dimensional location, specimen is reduced in body state;
Step 2: as shown in Fig. 2 (a)-Fig. 2 (c), read preoperative PET/CT image again, forming transverse section in PET/CT image between tracer uptake highest level and knuckle (is lung inner disease foci in the present embodiment, so selection is knuckle; As being intracerebral lesion scanning, then select the blood vessel that shows of specimen), specimen is cut from centre along this cross-sectional direction cutting in specimen plate, then to immerse in formalin 8 hours; As Fig. 3 (a) and Fig. 3 (b) is depicted as pathology aspect corresponding to PET/CT;
Step 3: shown in Fig. 4 (a) He Fig. 4 (b), makes the HE dyeing pathological section of specimen; Concrete grammar, for specimen is obtained multiple lamellar specimen cutting specimen plate cuts by the continuous parallel lamellar of PET/CT thickness, measures the actual (real) thickness of each lamellar specimen and number consecutively, by lamellar specimen conveniently pathological process make HE and to dye pathological section; The thickness of each lamellar specimen is 4.5mm, corresponding with the thickness of PET/CT;
Step 4: the wax stone region determining immunohistochemical staining region in HE dyeing pathological section under the microscope; As shown in Fig. 5 (a) He Fig. 5 (b), concrete grammar is determine region corresponding with PET/CT tracer region of interest on HE dyeing pathological section, region in being cut into slices by HE within tumor boundaries and in tracer region of interest is defined as the wax stone region in immunohistochemical staining region, as shown in Fig. 5 (c);
Step 5: each aspect paraformaldehyde in wax stone region selected in described step 4 is fixed, then carries out paraffin section, recycle this paraffin section and carry out immunohistochemical staining and the analysis of weary oxygen label;
Step 6: the parameter in tracer height picked-up region in every one deck PET/CT image is carried out correlation analysis with the expression of corresponding aspect SABC.
In described step one and step 2 formalin used to be concentration of formaldehyde be 37% formalin.
Paraformaldehyde in described step 5 is the paraformaldehyde of 4% of stamen bio tech ltd, Shanghai hundred.
The present invention can guarantee the accuracy of the weary oxygen scope of pulmonary carcinoma that tracer PET/CT measures, degree and locus and Immunohistochemical Expression image registration.Adopt the method for image-accurate comparative study of specimens from pri-pathological examination, the grand design of PET/CT image and histopathology endogenous markers phenotype micro image are carried out post processing and accuracy registration.Then may be used for all kinds of parameter of oncobiology characteristic and the dependency of digital pathological image SABC 3 D stereo quantitative parameter of analyzing PET/CT detection.
By reference to the accompanying drawings the specific embodiment of the present invention is described although above-mentioned; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various amendment or distortion that creative work can make still within protection scope of the present invention.
Claims (10)
1. a PET/CT macroscopic view digital information method of mating with pathology microscopic information, is characterized in that, comprise the following steps:
Step one: be reduced to by Postoperative Specimen in body state, then immerses in formalin;
Step 2: form transverse section between tracer uptake highest level and adjacent anatomic landmark in PET/CT image, cuts specimen from centre along this cross-sectional direction, then immerses in formalin;
Step 3: the HE dyeing pathological section making specimen;
Step 4: the wax stone region determining immunohistochemical staining region in every a slice HE dyeing pathological section;
Step 5: wax stone region paraformaldehyde selected in above-mentioned steps four is fixed, then carries out paraffin section, recycle the endogenous markers immunohistochemical staining that this paraffin section carries out detecting;
Step 6: the parameter in tracer height picked-up region in every one deck PET/CT image is carried out correlation analysis with the expression of corresponding aspect SABC.
2. a kind of PET/CT macroscopic view digital information method of mating with pathology microscopic information as claimed in claim 1, it is characterized in that, the concrete grammar of described step one is that the tissue intending excision is carried out labelling X under body naturalness, Y, Z, form specimen after tissue is in vitro, by specimen according in body three-dimensional location, specimen is reduced in body state.
3. a kind of PET/CT macroscopic view digital information method of mating with pathology microscopic information as claimed in claim 1, is characterized in that, in described step one, and 16-24 hour in immersion formalin.
4. a kind of PET/CT macroscopic view digital information method of mating with pathology microscopic information as claimed in claim 1, is characterized in that, in described step 2, and 8-14 hour in immersion formalin.
5. a kind of PET/CT macroscopic view digital information method of mating with pathology microscopic information as claimed in claim 1, is characterized in that, in described step one and step 2 formalin used to be concentration of formaldehyde be 35% to 40% formalin.
6. a kind of PET/CT macroscopic view digital information method of mating with pathology microscopic information as claimed in claim 1, is characterized in that, in described step 2 and step 3, specimen is all cut cutting in specimen plate.
7. a kind of PET/CT macroscopic view digital information method of mating with pathology microscopic information as claimed in claim 1, it is characterized in that, the concrete grammar of described step 3 obtains multiple lamellar specimen for specimen being cut by the continuous parallel lamellar of PET/CT thickness, measure the actual (real) thickness of each lamellar specimen and number consecutively, lamellar specimen is made HE and to dye pathological section.
8. a kind of PET/CT macroscopic view digital information method of mating with pathology microscopic information as claimed in claim 7, it is characterized in that, the thickness of each lamellar specimen is corresponding with the thickness of PET/CT, is 4-5mm.
9. a kind of PET/CT macroscopic view digital information method of mating with pathology microscopic information as claimed in claim 1, it is characterized in that, the concrete grammar of described step 4 is determine that HE dyes region corresponding with tracer region of interest in PET/CT image on pathological section under the microscope, will HE cut into slices in region within tumor boundaries and in tracer region of interest be defined as the wax stone region in immunohistochemical staining region.
10. a kind of PET/CT macroscopic view digital information method of mating with pathology microscopic information as claimed in claim 1, it is characterized in that, the paraformaldehyde in described step 5 is the paraformaldehyde of 4%.
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Cited By (5)
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| CN106198597A (en) * | 2016-07-04 | 2016-12-07 | 中国科学院自动化研究所 | Calculating neuro anatomy processing method based on histology and super high field mr techniques |
| WO2018205691A1 (en) * | 2017-05-09 | 2018-11-15 | 南昌德漫多科技有限公司 | System for processing histopathological specimen |
| CN111122230A (en) * | 2019-12-04 | 2020-05-08 | 广州金域医学检验中心有限公司 | Pathological section sampling method, device, equipment and storage medium |
| CN112734710A (en) * | 2020-12-30 | 2021-04-30 | 上海睿刀医疗科技有限公司 | Device and system for constructing focus recognition model based on historical pathological information |
| CN114820432A (en) * | 2022-03-08 | 2022-07-29 | 安徽慧软科技有限公司 | Radiotherapy effect evaluation method based on PET and CT elastic registration technology |
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| CN106198597A (en) * | 2016-07-04 | 2016-12-07 | 中国科学院自动化研究所 | Calculating neuro anatomy processing method based on histology and super high field mr techniques |
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