CN113358440A - Full-automatic liquid-based cell slide making, dyeing, slide sealing and slide reading integrated machine and method - Google Patents
Full-automatic liquid-based cell slide making, dyeing, slide sealing and slide reading integrated machine and method Download PDFInfo
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- G—PHYSICS
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Abstract
The invention provides a full-automatic liquid-based cell slide making, dyeing, slide sealing and slide reading all-in-one machine and a method, wherein the all-in-one machine comprises a pretreatment system, a slide reading system and a slide processing system, wherein the pretreatment system is used for pretreating an acquired sample; the slice making and dyeing system is used for making slices and dyeing the pretreated sample; the mounting system is used for packaging the dyed sample; the scanning and reading system is used for scanning and reading the packaged sample to acquire liquid-based cell information; the grabbing system is used for grabbing the conversion of the sample among the systems; and the control system is used for controlling the pretreatment system, the film making and dyeing system, the film sealing system, the scanning and film reading system and the grabbing system. The all-in-one machine can realize full automation of liquid-based cell slide making, dyeing, slide sealing and slide reading, and does not need manual operation from the sample machine to the whole process of the all-in-one machine, thereby saving manpower and material resources, and having low equipment cost and small space occupancy rate.
Description
Technical Field
The invention relates to the technical field of biological sample analysis and detection, in particular to a full-automatic liquid-based cell slide making, dyeing, slide sealing and slide reading integrated machine and a method.
Background
Exfoliative cytology is a method for diagnosing by using normal exfoliative cells of human tissues, liquid-based cytology is a part of exfoliative cytology, and exfoliative cytology specimens which are difficult to process in a traditional mode are put into a medium liquid to remove interference components which influence diagnosis, such as blood, mucus and the like, so that the aim of improving the diagnosis efficiency is fulfilled. Compared with the traditional cervical smear examination by a cervical scraping smear examination, the liquid-based cytology examination obviously improves the satisfaction degree of the sample and the detection rate of abnormal cervical cells.
The examination of liquid-based cells typically involves: preparing a film, dyeing, packaging, reading the film and the like. The traditional steps of slice making and dyeing and the like are manually carried out, which requires a long time for a slice maker to work and causes the quality of the slide to be low due to the interference of human factors. The traditional film reading method is also used for observing information on a slide by a doctor through a microscope, so that the film reading efficiency is very low, and no information is archived.
There are some semi-automatic slice-making and dyeing machines to meet different use requirements, however, the degree of automation is not high, such as: the centrifugal tube is filled with the separating liquid manually, the centrifugal tube filled with the separating liquid, the pipetting injector and the sample are placed in a sample processor for pretreatment operation, then the centrifugal tube filled with the separating liquid and the sample to be detected is manually placed in a centrifuge for completing centrifugation, and then the centrifugal tube is manually taken out from the centrifuge, and the like.
Some existing slide scanners can scan information on a slide into a high-quality and high-definition image for a doctor to make a diagnosis, but scanning still cannot replace the doctor's reading work.
Disclosure of Invention
The invention aims to provide a full-automatic liquid-based cell slide making, dyeing, slide sealing and slide reading all-in-one machine aiming at the defects in the prior art. The all-in-one machine can realize full automation of liquid-based cell slide making, dyeing, slide sealing and slide reading, and does not need manual operation from the sample machine to the whole process of the all-in-one machine, thereby saving manpower and material resources, and having low equipment cost and small space occupancy rate.
The invention provides a full-automatic liquid-based cell slide making, dyeing, slide sealing and slide reading integrated machine, which comprises:
the pretreatment system is used for pretreating the collected sample; the slice making and dyeing system is used for making slices and dyeing the pretreated sample; the mounting system is used for packaging the dyed sample; the scanning and reading system is used for scanning and reading the packaged sample to acquire liquid-based cell information; the clamping jaw is used for switching the position of the sample among various systems; and the control system is used for controlling the pretreatment system, the film making and dyeing system, the film sealing system, the scanning and film reading system and the clamping jaws.
Preferably, the pretreatment system comprises: the sample code scanning module is used for scanning and archiving the collected samples; the oscillation module is used for oscillating and uniformly mixing the sample; the sampling module is used for transferring the sample into a centrifugal tube; the centrifugal module is used for carrying out centrifugal processing on the sample to obtain a cell solution; and the waste liquid transfer module is used for removing the upper layer waste liquid in the centrifugal tube.
Preferably, the slide staining system comprises: the code spraying module is used for spraying the information acquired from the sample code scanning by the sample code scanning module onto the glass slide; the slide making module is used for enabling the cell nucleus to be adhered to the slide through the sedimentation tube and fixing the cell nucleus; the staining module is used for staining the slide adhered with the cell nucleus; and the air drying module is used for air drying the dyed slide.
Preferably, the mounting system comprises: the adding module is used for adding gum to the dyed and air-dried glass slide and covering a cover glass; and the tabletting module is used for tabletting and packaging the sample added with the gum and covered with the cover slip.
Preferably, the scanning and reading system comprises: the scanning module is used for scanning the packaged sample to obtain a panoramic slide image; and the slide reading module is used for reading the panoramic slide image to acquire liquid-based cell information.
Further preferably, the scanning module includes: the objective table is used for placing the sample after tabletting and packaging; the objective table control unit is used for controlling the movement of the objective table; a dual objective unit for focusing the encapsulated sample; and the acquisition unit is used for acquiring code spraying information and a panoramic slide image on the slide.
The invention also provides a full-automatic liquid-based cell slide making, dyeing, slide sealing and slide reading method, which comprises the following steps: s1, preprocessing a collected sample by a preprocessing system; s2, the film making and dyeing system makes the pretreated sample undergo the processes of film making and dyeing; s3, packaging the dyed sample by using a piece sealing system; and S4, scanning and reading the packaged sample by using a scanning and reading system to obtain liquid-based cell information.
Preferably, step S1 includes: s11, a sample code scanning module scans and archives the collected sample; s12, a vibration module is used for uniformly vibrating and mixing the collected samples; (ii) a S13, a sampling module samples a part of the sample uniformly mixed by oscillation and transfers the part of the sample into a centrifuge tube; s14, centrifuging the sample by using a centrifugal module to obtain a cell solution; s15, the upper layer waste liquid in the centrifugal tube is removed by the waste liquid transfer module.
Preferably, step S2 includes: s21, the code spraying module sprays the information obtained by the sample code scanning module from the sample code scanning onto the glass slide; s22, a sheet making module is used for enabling cell nucleuses to be adhered to the glass sheet through the sedimentation tube and fixing the cell nucleuses; s23, dyeing the slide adhered with the cell nucleus by using a dyeing module; and S24, air-drying the dyed glass slide by an air-drying module.
Preferably, the step S3 includes: adding gum to the dyed and air-dried glass slide by an adding module and covering a cover glass; the tabletting module tablet-encapsulates the gum-added and coverslipped specimen.
Preferably, the step S4 includes: the scanning module scans the packaged sample to obtain a panoramic slide image; and the slide reading module reads the panoramic slide image to acquire liquid-based cell information.
The invention has the beneficial effects that: by means of linkage control of the systems and free switching of samples among the systems by the grabbing system, full automation of liquid-based cell slide making, dyeing, slide sealing and slide reading can be achieved, manual operation is not needed in the whole process from sample installation to the integrated machine, manpower and material resources are saved, and the device is low in cost and small in space occupancy rate.
Drawings
FIG. 1 is a system framework structure diagram of the all-in-one machine in the embodiment of the invention.
Fig. 2 is a schematic perspective view of an all-in-one machine according to an embodiment of the invention.
FIG. 3 is a side view of an embodiment of the kiosk of the present invention.
Fig. 4 is an explosion structure diagram of the all-in-one machine in the embodiment of the invention.
Fig. 5 is a schematic diagram of the automated process steps of the all-in-one machine in the embodiment of the invention.
Fig. 6 is a schematic process diagram of a screening method for cervical cell images used for radiographing in an embodiment of the present invention.
1, an integrated machine, 101 an oscillation module and 102 a centrifugal module; a code spraying module 201, a settling area 202, an air drying module 203 and a slide rack 204; 30 a mounting system; 40 scanning and reading the film system; 60 a grasping system.
Detailed Description
The present invention is described in further detail below with reference to specific embodiments and with reference to the attached drawings, it should be emphasized that the following description is only exemplary and is not intended to limit the scope and application of the present invention.
The embodiment provides a full-automatic liquid-based cytology specimen preparing, staining, sealing and reading all-in-one machine, as shown in fig. 1-4, which comprises a pretreatment system 10 for pretreating a collected sample; a slice-making and staining system 20 for making slices and staining the pretreated sample; a mounting system 30 for mounting the stained specimen; the scanning and reading system 40 is used for scanning and reading the packaged sample to acquire liquid-based cell information; a grasping system 60 for grasping the transition of the sample between the systems; and the control system 50 is used for controlling the pretreatment system 10, the film making and dyeing system 20, the film sealing system 30, the scanning and film reading system 40 and the grabbing system. Wherein the gripping system may be a jaw.
Specifically, the pretreatment system 10 includes: the sample code scanning module is used for scanning and archiving the collected samples; the oscillation module 101 is used for oscillating and uniformly mixing the sample; the sampling module is used for transferring the sample into a centrifugal tube; a centrifugation module 102 for centrifuging the sample to obtain a cell solution; and the waste liquid transfer module is used for removing the upper layer waste liquid in the centrifugal tube.
The slide-making staining system 20 includes: the code spraying module 201 is used for spraying the information obtained by the sample code scanning module from the sample code scanning onto the glass slide; the slide making module is used for enabling the cell nucleus to be adhered to the slide through the sedimentation tube and fixing the cell nucleus; the staining module is used for staining the slide adhered with the cell nucleus; and the air drying module is used for air drying the dyed slide.
The mounting system 30 includes: the adding module is used for adding gum to the dyed and air-dried glass slide and covering a cover glass; and the tabletting module is used for tabletting and packaging the sample added with the gum and covered with the cover slip.
The scanning and film reading system comprises: the scanning module is used for scanning the packaged sample to obtain a panoramic slide image; and the slide reading module is used for reading the panoramic slide image to acquire liquid-based cell information.
Wherein, the scanning module includes: the objective table is used for placing the sample after tabletting and packaging; the objective table control unit is used for controlling the movement of the objective table; a dual objective unit for focusing the encapsulated sample;
and the acquisition unit is used for acquiring code spraying information and a panoramic slide image on the slide.
The method for carrying out the automatic operation flow of the liquid-based cells by utilizing the integrated machine comprises the following steps:
s1, preprocessing a collected sample by a preprocessing system; s2, the film making and dyeing system makes the pretreated sample undergo the processes of film making and dyeing; s3, packaging the dyed sample by using a piece sealing system; and S4, scanning and reading the packaged sample by using a scanning and reading system to obtain liquid-based cell information.
Specifically, as shown in fig. 5, the following are included:
after the system of the all-in-one machine is initialized, firstly, the sample bottles are placed on the sample table one by one, then the clamping jaws clamp the sample bottles, the sample scanning module scans codes, information of each sample is read, and the information is stored in a computer. After the code scanning is finished, the sample bottles are uniformly placed on an oscillator of the oscillation module according to a certain sequence to be oscillated and uniformly mixed. Utilize the straw to absorb the cell separation liquid of fixing on the all-in-one (being worth noting, can carry out self-cleaning after the straw has absorbed each time), pour into appropriate amount cell separation liquid in each centrifuge tube on the centrifugal module, in the sample of reuse straw extraction sample bottle adds the centrifuge tube, sample in the sample bottle takes away the back, and the sample bottle can fall into in the abandonment bucket of oscillator below automatically.
The centrifugal tube is subjected to primary centrifugation operation for a certain time, and after the primary centrifugation is finished, the upper-layer waste liquid in the centrifugal tube is absorbed by the waste liquid transfer module and is discarded; adding a proper amount of cleaning solution into the centrifugal pipe by using the cleaning solution guide pipe, and sucking the cleaning solution by using the waste liquid transfer module and discarding the cleaning solution after cleaning; and pouring cell separation liquid into the centrifugal tube to perform secondary centrifugal operation, and sucking the upper-layer waste liquid by using a waste liquid transfer module and discarding the upper-layer waste liquid after the secondary centrifugal operation. And (3) placing the centrifuge tube in an oscillator after the secondary centrifugation is finished, extracting the buffer solution fixed on the integrated machine by using a suction tube, pouring a proper amount of buffer solution into the centrifuge tube, and oscillating for a certain time by the oscillator to obtain a cell solution for later use.
When the sample bottles are scanned and stored, the empty slide is also placed on the slide rack 204, and the slide pushing device is firstly used for pushing the empty slide to the guide rail slide taking area and then translating the empty slide to the code spraying area of the code spraying module. The information scanned from the sample bottle is printed on the corresponding glass slide by an ink-jet device, then the glass slide is moved out to be close to the clamping jaw area, and a piece of glass slide is placed in the sedimentation area 202 by the clamping jaw to wait for the production of the slide. Meanwhile, the settling tube is placed on the settling area and fixed well and placed horizontally.
Sucking a proper amount of the vibrated cell solution by using a suction pipe and putting the cell solution into a settling tube; care must be taken to ensure that each settling tube is vertical and in the same horizontal line. Because cells are heavier due to enlargement of diseased cell nuclei and maladjustment of nucleus pulposus proportion, the cells can be settled more quickly in liquid, so that in the separated cell liquid, the diseased cells can reach the slide glass with adhesive force for a certain time and then the liquid in the settling tube is sucked by the waste liquid transfer module; and then, an alcohol catheter is used for sucking 95% alcohol into the settling tube for a fixed period of time, and the alcohol in the settling tube is sucked again and is placed for a certain period of time for dyeing.
After a suction pipe is used for sucking staining liquid, the staining liquid is added into a settling tube and is reserved for a certain time, a waste liquid transfer module sucks the staining liquid, fixing liquid is added finally, liquid above the liquid is immediately sucked and is placed for a period of time, the settling tube is slightly moved upwards, a glass slide with a sample is taken out, and the glass slide is placed in an air drying module by using a clamping jaw to be air-dried.
After air drying is completed, the slides are placed on the mounting system using the clamping jaws, the air dried slides are first added with neutral gum by the addition module and covered with a coverslip. And (4) subsequently, tabletting and packaging are carried out by a tabletting module, and no bubbles and gum overflow are ensured after the tabletting is sealed.
After the mounting is finished, a clamping jaw places a slide on an objective table, the objective table is controlled to move by an X/Y axis control module, an A axis control module drives real double objectives to switch, a multiple objective lens needing scanning is selected, firstly, an identification module in a scanning module reads bar code information on the slide and a slide information removing range, then an acquisition module starts to focus and scan slide information, a Z axis drive module is matched with a piezoelectric ceramic control acquisition system to move and acquire hundreds of thousands of small images, and then, the small images are spliced by utilizing an algorithm to form a high-quality high-definition panoramic slide image. The acquisition process comprises the following steps: the power switch is turned on for initialization and the control card sends commands to each driver to drive the respective motor to bring the X, Y, Z axis to the origin of the system settings. At this time the X/Y axis controls the stage ejection for slide placement. In the first step, the control card sends an instruction to the A-axis driving module to switch the objective lens according to the requirement. And the second step is that the control card sends an instruction to the X/Y axis module to move the object stage to the position below the identification module to carry out two-dimensional code identification and determine the scanning range of the slide. At this time the Z-axis is ramped up to confirm that there is sufficient space under the objective lens. And thirdly, after the scanning range is determined, the objective table is moved to the position below the acquisition camera for focusing. When the piezoelectric ceramic is fixed at a first point, the Z axis drives the piezoelectric ceramic and the acquisition module to move up and down for focusing, and after the Z axis is roughly focused to determine a clear point, the single chip microcomputer controls the piezoelectric ceramic to drive the acquisition module to move up and down for precise fine focusing to determine a clearer point. And then, next point focusing is carried out to find the clearest point until the point focusing is completed. And fourthly, scanning after focusing is finished, wherein the scanning mode is to perform S-shaped scanning on a line by a line on the whole image.
And after the panoramic slide image is scanned and obtained, the panoramic slide image is read by using the reading module, so that the liquid-based cell information is obtained. The method for screening cervical cell images is used for reading, and comprises the following steps:
t1, preprocessing an original image containing cervical cells;
t2, establishing a cervical cell image screening model and training;
and T3, inputting the image preprocessed in the step T1 into the established cervical cell model in the step T2 to screen the cervical cell image.
The preprocessing of step T1 includes: t11, performing cell nucleus segmentation on the original image to obtain a cell image block; and T12, performing data enhancement on the cell image block.
In the cervical cell image, the cell nucleus contains rich physiological information, which is one of the key judgment bases for classification. Morphologically, the nucleus of a cell has the characteristics of dense texture, dark color and generally regular round shape. The difficulty of segmenting the nucleus is somewhat lower than segmenting the entire cervical cell. However, dirt, defocusing artifacts and inflammatory cells existing on the glass slide are easy to be confused with cell nuclei, and in order to eliminate the influence of the problems, the cell nuclei are divided into two steps, wherein invalid information is removed in the first step, and the cell image blocks are divided in the second step by using an automatic division network.
Fig. 6 shows a schematic diagram of a process for removing invalid information from an original image, where the invalid information includes dirt, defocus artifacts, inflammatory cells, and the like. Since the dirt is generally grey, these impurities are eliminated by setting a threshold for the absolute channel difference in the RGB color space. For defocus artifacts, the boundaries of poorly focused objects are usually blurred, and therefore the boundaries are removed by thresholding the average gradient of the boundaries using the Sobel operator. Finally, inflammatory cells with small dark nuclei are eliminated by adjusting the thresholded maximum radius Rmax and the mean intensity Imean. The threshold parameters are manually adjusted by experience. The parameters selected can eliminate as many artifacts as possible while retaining nearly all true cervical cells.
And then segmenting the cervical cell image after invalid information is removed. Due to the good effect of the U-Net structure on the segmentation problem of the medical image, the basic idea is still adopted in the part of the nuclear segmentation work.
After obtaining the image without invalid information, taking the center of mass of the cell nucleus as the center, extracting image blocks with the same size (such as 224 × 224) as the segmented cell image blocks. This strategy may embed not only scale/size information of the nucleus, but also contextual cues (e.g., cytoplasm) in the extracted patches. Compared with a sliding window method, more effective training data can be obtained.
Since labeling of cervical cancer cells is dependent on a professional pathologist, the amount of data is limited. In addition, in practical application scenarios, the number of negative cells is far greater than that of positive cells, and positive and negative sample imbalance exists. This example therefore performed data enhancement on positive cells. Because cervical cells have rotational invariance, a rotation operation (the step length is theta degrees) is performed on each cell image block containing positive cells in the segmented cell image, and in addition, considering that the actually detected nuclear center may be inaccurate, each nuclear center is randomly translated for Nt times to obtain an Nt point as a coarse nuclear center, so that the number of positive and negative samples is about 1: 1. Data enhancement increases the number of image samples, improves the accuracy of the network, and reduces overfitting.
In step T2, the present embodiment applies the idea of the transfer learning. For convolutional neural networks, transfer learning is the successful application of the "knowledge" trained on a particular data set to a new domain. The training process comprises (1) completing the pre-training of the convolutional neural network on a data set ImageNet; (2) and training the cervical cell image screening model by using the pre-trained convolutional neural network so as to finely adjust the parameters.
The training of the model is a training database constructed based on huge numbers of liquid-based cell images, and as a slide scanner is used for scanning slides in hospitals and schools, a mature database is provided, and data in the database gives corresponding labels according to clinical knowledge of doctors. Inputting suspected diagnosis into an existing initial model in a model training process, diagnosing cells in a small area by the initial model, judging that a model screening result is correct when the given diagnosis is consistent with the diagnosis judged by a doctor, feeding back information when the given diagnosis is not consistent with the diagnosis judged by the doctor, modifying the model to improve the screening and classifying accuracy, screening and diagnosing by a high-accuracy model, classifying according to the types of the cells, observing the conditions of the cells by the user through the screened classification, and finally giving the diagnosis in a diagnosis report derived by a system.
Although there are many classification networks in the convolutional neural network, the convolutional neural network model in the embodiment selects the VGG convolutional neural network, which has the best performance and the shortest prediction time compared with the convolutional neural networks such as Resnet and densnet. The present embodiment determines the network structure of VGG19, namely: 19 hidden layers (16 convolutional layers and 3 fully-connected layers) were included, all with a small convolution kernel of 3x3, with the convolutional layer step size set to 1. Replacing the convolution kernel of 5x5 or 7x7 with a small convolution kernel of 3x3 can increase nonlinearity while reducing the number of parameters; and the structure is simple, and the performance is excellent.
The present embodiment also verifies the superiority of the block generation method based on the nuclear segmentation over the sliding window method. To prevent the effect of random errors, quintupled cross-validation was used to evaluate the effect of the model, i.e. 4 times in each 5 iterations as training data and the remaining one as validation data, and the final performance value of the model was obtained by averaging the results of the 5 validation sets. Performance assessment indicators include Accuracy (Accuracy), Specificity, Sensitivity (Sensitivity), F1-score, where Accuracy is the global percentage of correctly classified pictures, Specificity measures the proportion of correctly identified normal cells, Sensitivity measures the proportion of correctly identified abnormal cells, and F1-score refers to the harmonic mean of precision and recall. The results of the performance evaluation indexes are as follows:
| Method | rate of accuracy | Rate of accuracy | Sensitivity of the composition | F1 value |
| Nucleus cutting map | 0.983±0.02 | 0.983±0.03 | 0.983±0.02 | 0.983±0.04 |
| Cutting picture by sliding window method | 0.937±0.01 | 0.942±0.02 | 0.937±0.03 | 0.936±0.02 |
Through the all-in-one of this embodiment, the slide of preparing is of high quality, and the panorama slide image that scans out is clear, and need not the manual work and read the piece, and the piece is read to intelligence efficient and the precision height.
The foregoing is a more detailed description of the invention in connection with specific/preferred embodiments and is not intended to limit the practice of the invention to those descriptions. It will be apparent to those skilled in the art that various substitutions and modifications can be made to the described embodiments without departing from the spirit of the invention, and these substitutions and modifications should be considered to fall within the scope of the invention.
Claims (10)
1. The utility model provides a full-automatic liquid-based cell film-making, dyeing, mounting, read piece all-in-one which characterized in that includes:
the pretreatment system is used for pretreating the collected sample;
the slice making and dyeing system is used for making slices and dyeing the pretreated sample;
the mounting system is used for packaging the dyed sample;
the scanning and reading system is used for scanning and reading the packaged sample to acquire liquid-based cell information;
the grabbing system is used for grabbing the conversion of the sample among the systems;
and the control system is used for controlling the pretreatment system, the film making and dyeing system, the film sealing system, the scanning and film reading system and the grabbing system.
2. The all-in-one machine of claim 1, wherein the pre-processing system comprises:
the sample code scanning module is used for scanning and archiving the collected samples;
the oscillation module is used for oscillating and uniformly mixing the sample;
the sampling module is used for transferring the sample into a centrifugal tube;
the centrifugal module is used for carrying out centrifugal processing on the sample to obtain a cell solution;
and the waste liquid transfer module is used for removing the upper layer waste liquid in the centrifugal tube.
3. The integrated machine of claim 1, wherein the production staining system comprises:
the code spraying module is used for spraying the information acquired from the sample code scanning by the sample code scanning module onto the glass slide;
the slide making module is used for enabling the cell nucleus to be adhered to the slide through the sedimentation tube and fixing the cell nucleus;
the staining module is used for staining the slide adhered with the cell nucleus;
and the air drying module is used for air drying the dyed slide.
4. The integrated machine of claim 1, wherein the mounting system comprises:
the adding module is used for adding gum to the dyed and air-dried glass slide and covering a cover glass;
and the tabletting module is used for tabletting and packaging the sample added with the gum and covered with the cover slip.
5. The all-in-one machine of claim 1, wherein the scanning and interpreting system comprises:
the scanning module is used for scanning the packaged sample to obtain a panoramic slide image;
and the slide reading module is used for reading the panoramic slide image to acquire liquid-based cell information.
6. The all-in-one machine of claim 5, wherein the scanning module comprises:
the objective table is used for placing the sample after tabletting and packaging;
the objective table control unit is used for controlling the movement of the objective table;
a dual objective unit for focusing the encapsulated sample;
and the acquisition unit is used for acquiring code spraying information and a panoramic slide image on the slide.
7. A full-automatic liquid-based cell slide making, staining, slide sealing and slide reading method is characterized by comprising the following steps:
s1, preprocessing a collected sample by a preprocessing system;
s2, the film making and dyeing system makes the pretreated sample undergo the processes of film making and dyeing;
s3, packaging the dyed sample by using a piece sealing system;
and S4, scanning and reading the packaged sample by using a scanning and reading system to obtain liquid-based cell information.
8. The method of claim 7, wherein the step S1 includes:
s11, a sample code scanning module scans and archives the collected sample;
s12, a vibration module is used for uniformly vibrating and mixing the collected samples; (ii) a
S13, a sampling module samples a part of the sample uniformly mixed by oscillation and transfers the part of the sample into a centrifuge tube;
s14, centrifuging the sample by using a centrifugal module to obtain a cell solution;
s15, the upper layer waste liquid in the centrifugal tube is removed by the waste liquid transfer module.
9. The method of claim 7, wherein the step S2 includes:
s21, the code spraying module sprays the information obtained by the sample code scanning module from the sample code scanning onto the glass slide;
s22, a sheet making module is used for enabling cell nucleuses to be adhered to the glass sheet through the sedimentation tube and fixing the cell nucleuses;
s23, dyeing the slide adhered with the cell nucleus by using a dyeing module;
and S24, air-drying the dyed glass slide by an air-drying module.
10. The method of claim 7, wherein the step S3 includes:
adding gum to the dyed and air-dried glass slide by an adding module and covering a cover glass;
the tabletting module tablet-encapsulates the gum-added and coverslipped specimen.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113740138A (en) * | 2021-09-11 | 2021-12-03 | 南京泰立瑞信息科技有限公司 | Non-contact low-consumption rapid section staining method and system |
| CN113790940A (en) * | 2021-09-08 | 2021-12-14 | 深圳市森盈生物科技有限公司 | Serial-type waste liquid is clear away and automatic temperature control system |
| WO2023109914A1 (en) * | 2021-12-15 | 2023-06-22 | 深圳市瑞图生物技术有限公司 | Liquid-based slide preparation device and slide preparation method of sample |
| CN116793799A (en) * | 2023-07-17 | 2023-09-22 | 北京华伊智能医疗科技有限公司 | Full-automatic cell slice-making, dyeing and slice-sealing integrated device |
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2021
- 2021-06-28 CN CN202110718954.6A patent/CN113358440A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113790940A (en) * | 2021-09-08 | 2021-12-14 | 深圳市森盈生物科技有限公司 | Serial-type waste liquid is clear away and automatic temperature control system |
| CN113740138A (en) * | 2021-09-11 | 2021-12-03 | 南京泰立瑞信息科技有限公司 | Non-contact low-consumption rapid section staining method and system |
| CN113740138B (en) * | 2021-09-11 | 2024-03-26 | 南京泰立瑞信息科技有限公司 | Non-contact low-consumption rapid slice dyeing method and system |
| WO2023109914A1 (en) * | 2021-12-15 | 2023-06-22 | 深圳市瑞图生物技术有限公司 | Liquid-based slide preparation device and slide preparation method of sample |
| CN116793799A (en) * | 2023-07-17 | 2023-09-22 | 北京华伊智能医疗科技有限公司 | Full-automatic cell slice-making, dyeing and slice-sealing integrated device |
| CN116793799B (en) * | 2023-07-17 | 2024-03-12 | 北京华伊智能医疗科技有限公司 | Full-automatic cell slice-making, dyeing and slice-sealing integrated device |
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