CN117367923B - Device and method for processing oral cancer in-vitro tumor tissue specimens - Google Patents
Device and method for processing oral cancer in-vitro tumor tissue specimens Download PDFInfo
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- CN117367923B CN117367923B CN202311681966.1A CN202311681966A CN117367923B CN 117367923 B CN117367923 B CN 117367923B CN 202311681966 A CN202311681966 A CN 202311681966A CN 117367923 B CN117367923 B CN 117367923B
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- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 47
- 208000003445 Mouth Neoplasms Diseases 0.000 title claims abstract description 29
- 208000012987 lip and oral cavity carcinoma Diseases 0.000 title claims abstract description 29
- 238000000338 in vitro Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 117
- 238000004140 cleaning Methods 0.000 claims abstract description 99
- 238000004043 dyeing Methods 0.000 claims abstract description 88
- 238000005192 partition Methods 0.000 claims abstract description 62
- 238000000926 separation method Methods 0.000 claims abstract description 11
- 239000007921 spray Substances 0.000 claims description 25
- 238000007789 sealing Methods 0.000 claims description 9
- 238000005507 spraying Methods 0.000 claims description 8
- 230000037452 priming Effects 0.000 claims description 5
- 230000005611 electricity Effects 0.000 claims description 4
- 238000004061 bleaching Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 230000002055 immunohistochemical effect Effects 0.000 claims description 3
- 230000001575 pathological effect Effects 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 3
- 210000001519 tissue Anatomy 0.000 description 32
- 238000009434 installation Methods 0.000 description 7
- 238000002791 soaking Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000003466 welding Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 206010041823 squamous cell carcinoma Diseases 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000003116 impacting effect Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
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- Physics & Mathematics (AREA)
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Abstract
The invention is suitable for the technical field of tissue specimen detection, and provides an in-vitro tumor tissue specimen treatment device and a treatment method for oral cancer, wherein the treatment device comprises a box body, a partition plate, a dip dyeing cavity, a cleaning cavity, a mounting plate, a sector gear, a rack, a net frame and a moving mechanism; after the dip dyeing treatment, the separation plate is driven to move towards one side of the cleaning cavity through the moving mechanism, one end of the mounting plate, which is positioned in the cleaning cavity, descends into the cleaning liquid, the screen frame slides into the cleaning cavity, and enters the cleaning liquid, so that the transfer of a tumor tissue specimen from the dip dyeing treatment to the cleaning treatment is automatically and rapidly completed, the transfer is not required to be manually operated, the working efficiency is improved, the dip dyeing cavity and the cleaning cavity are separated, and the mutual influence between the dip dyeing treatment and the cleaning treatment is reduced.
Description
Technical Field
The invention belongs to the technical field of tissue specimen detection, and particularly relates to an isolated tumor tissue specimen treatment device and method for oral cancer.
Background
Oral cancer is a generic term for malignant tumors occurring in the oral cavity, mostly belonging to squamous cell carcinoma, so-called mucosal variant; the oral cancer needs to carry out pathological diagnosis process, and a tumor tissue specimen needs to be obtained and then treated;
according to the search, the patent application with the publication number of CN113820193A discloses an in-vitro tumor tissue sample treatment device for oral squamous carcinoma, which comprises a bearing component, a turnover component, a spraying component and a soaking box, wherein the bearing component is rotatably arranged in the soaking box through the turnover component and is close to the box opening of the soaking box, and the spraying component is fixedly arranged above the bearing component; the bearing assembly comprises a cross clamping rod, a bearing clamping box and a driving assembly, wherein the bearing clamping box comprises two groups of hollow shells which are arranged in a hemispherical shape, the two groups of hollow shells are respectively and symmetrically arranged at the top of the cross clamping rod, and small holes are annularly and equidistantly arranged in the hollow shells;
among the above-mentioned prior art, through setting up soaking box, upset subassembly and spray the subassembly, carry out tissue specimen and handle, on the one hand, whole device is in full open state, and cross influence takes place easily with the external world, volatilizees, external dirt's entering like liquid, probably produces adverse effect, on the other hand, sprays and soaks the intercommunication, and the liquid of spraying directly flows into soaking box, takes place to mix, and follow-up if need continue to handle then can influence the treatment effect.
Disclosure of Invention
The invention provides an isolated tumor tissue sample processing device for oral cancer, which aims to solve the problems.
The invention is realized in this way, a kind of oral cancer tumor tissue sample processing unit that isolated, comprising:
a case;
the separation plate is arranged in the box body and is used for separating the inner cavity of the box body into a dip dyeing cavity and a cleaning cavity, a plurality of horizontal guide rods are arranged on the edge of the separation plate in a penetrating manner, and the guide rods are fixedly connected with the side wall of the box body;
the mounting plate penetrates through the partition plate, the middle part of the mounting plate is rotationally connected with the partition plate through a rotating shaft, two ends of the mounting plate are respectively positioned in the dip dyeing cavity and the cleaning cavity, one end of the rotating shaft extends to the outside of the box body and is fixedly provided with a sector gear, a rack is fixed on the outer wall of the box body, the rack is positioned at the lower side of the sector gear and meshed with the sector gear, and a strip groove for the rotation shaft to move is formed in the outer wall of the box body;
the screen frame is slidably arranged on the lower surface of the mounting plate and used for placing tumor tissue specimens, and the partition plate is provided with through holes for the screen frame to pass through;
and the moving mechanism is arranged on the box body and used for driving the partition plate to move horizontally.
Preferably, sealing strips are arranged on the bottom of the partition plate and the front and rear side walls, liquid inlet pipes and liquid outlet pipes are respectively connected to the two side walls of the box body, and valves are arranged on the liquid inlet pipes and the liquid outlet pipes.
Preferably, the top of the net frame is hinged with a sliding seat, the sliding seat is in sliding connection with a sliding groove formed in the lower surface of the mounting plate, and two ends of the sliding groove are closed.
Preferably, the net frame is formed by detachable surrounding of two half frames, one ends of the two half frames are rotationally connected, and the other ends of the two half frames are connected through buckles.
Preferably, the moving mechanism includes:
the screw rod is horizontally rotatably arranged at the top of the box body;
the first stepping motor is fixed on the box body and is used for driving the screw rod to rotate;
the mounting seat is in threaded connection with the screw rod, the mounting seat is in driving connection with the partition plate, and the mounting seat is in sliding connection with the top of the box body.
Preferably, the mounting seat is connected with the partition plate through a driving mechanism, and the driving mechanism comprises:
the second stepping motor is fixed on the mounting seat;
a turntable fixed on the output shaft of the second stepping motor;
the driving pin is eccentrically arranged on the turntable, and the vertical movable groove for the driving pin to be inserted and move is formed in the partition plate.
Preferably, the rotary table is rotatably provided with a threaded rod, the threaded rod is radially arranged along the rotary table, the threaded rod is connected with an internal thread sleeve in a threaded manner, the internal thread sleeve is in sliding connection with the rotary table, and the driving pin is fixed on the internal thread sleeve.
Preferably, a plurality of spouting pieces are installed from top to bottom at intervals on the two side walls of the partition plate, the spouting pieces comprise a mounting rod and a spray head, one end of the mounting rod is hinged to the side wall of the partition plate, the spray head is installed at the other end of the mounting rod and used for spraying liquid to the net frame, a liquid supply assembly is arranged in the box body and used for supplying liquid to the spray head, a power mechanism used for driving the mounting rod to swing up and down is arranged in the box body, and the power mechanism comprises:
a connecting rod rotatably mounted on the drive pin;
the driving rods are fixed at two ends of the connecting rod, the two driving rods are respectively connected with the mounting rods on two side walls of the partition plate in a matched mode, the driving rods are vertically arranged and are in sliding connection with the side walls of the partition plate, a plurality of pin shafts which are in one-to-one correspondence with the mounting rods are fixed on the driving rods, and through grooves for the pin shafts to be inserted and move are formed in the mounting rods.
Preferably, the liquid supply assembly comprises:
the self-priming pump is fixedly arranged on the partition plate and positioned below the mounting plate;
two liquid suction pipes connected to the inlet of the self-priming pump, wherein the two liquid suction pipes extend to the lower parts of the dip dyeing cavity and the cleaning cavity respectively, and electromagnetic valves are arranged on the two liquid suction pipes respectively;
two liquid supply pipes connected to the outlet of the self-priming pump, wherein the two liquid supply pipes are respectively communicated with the spray heads in the dip dyeing cavity and the cleaning cavity, and electromagnetic valves are respectively arranged on the two liquid supply pipes;
the left and right sides symmetry is installed on the division board and is located two trigger switch of mounting panel downside, two trigger switch's bottom is connected with the division board rotation and is connected with reset torsion spring between the division board, two trigger switch's top extends to the division board downside to both sides, and two trigger switch are connected with the self priming pump electricity respectively, can control the start-stop of self priming pump, and every trigger switch is connected with the solenoid valve electricity on the pipette and the feed pipe of homonymy, can control the switching of solenoid valve.
The invention also provides a processing method based on the oral cancer in-vitro tumor tissue sample processing device, which comprises the following steps:
obtaining an in-vitro tumor tissue sample of the oral cancer, and placing the in-vitro tumor tissue sample in a net frame;
adding a dip dyeing liquid into the dip dyeing cavity, and adding a cleaning liquid into the cleaning cavity;
the partition plate is driven to move to one side of the dip dyeing cavity by the moving mechanism, the sector gear and the rack move relatively, the sector gear rotates anticlockwise, the sector gear drives the mounting plate to rotate anticlockwise by the rotating shaft, one end of the mounting plate positioned in the dip dyeing cavity and the net frame move downwards, and the mounting plate enters into dip dyeing liquid to be dip dyed for 1-2h;
after dip dyeing treatment, the partition plate is driven to move to one side of the cleaning cavity by the moving mechanism, meanwhile, the sector gear rotates clockwise, the sector gear drives the mounting plate to rotate clockwise by the rotating shaft, one end of the mounting plate, which is positioned in the cleaning cavity, descends into the cleaning liquid, and in the rotating process of the mounting plate, the screen frame slides along the mounting plate from the dip dyeing cavity into the cleaning cavity, enters into the cleaning liquid and is cleaned for 2-4min;
after the cleaning treatment, slicing and bleaching a tumor tissue specimen to obtain a specimen slice;
and acquiring images of the specimen slices through an image scanning acquisition unit, and performing pathological section examination and immunohistochemical examination.
Compared with the prior art, the embodiment of the application has the following main beneficial effects:
according to the device for treating the oral cancer in-vitro tumor tissue specimens, provided by the invention, a box body, a separation plate, a dip dyeing cavity, a cleaning cavity, a mounting plate, a sector gear, a rack, a net frame and a moving mechanism are arranged, the separation plate is driven to move to one side of the dip dyeing cavity by the moving mechanism, meanwhile, the sector gear and the rack are relatively moved, the sector gear is rotated anticlockwise, the sector gear drives the mounting plate to rotate anticlockwise by a rotating shaft, and one end of the mounting plate, which is positioned in the dip dyeing cavity, and the net frame move downwards and enter into dip dyeing liquid; after the dip dyeing treatment, the partition plate is driven to move towards one side of the cleaning cavity through the moving mechanism, meanwhile, the sector gear rotates clockwise, the sector gear drives the mounting plate to rotate clockwise through the rotating shaft, one end of the mounting plate, which is positioned in the cleaning cavity, descends into the cleaning fluid, in the process of rotating the mounting plate, the net frame slides along the mounting plate from the dip dyeing cavity to the cleaning cavity, enters the cleaning fluid, automatically and quickly completes the transfer of a tumor tissue specimen from the dip dyeing treatment to the cleaning treatment, the transfer is not required to be manually operated, the working efficiency is improved, the dip dyeing cavity and the cleaning cavity are separated, and the mutual influence between the dip dyeing treatment and the cleaning treatment is reduced.
Drawings
FIG. 1 is a schematic diagram of a device for processing an isolated tumor tissue sample of an oral cancer;
FIG. 2 is a side view of FIG. 1;
FIG. 3 is a view showing the fit intention of a sector gear and a rack in an in-vitro tumor tissue sample processing device for oral cancer;
fig. 4 is an enlarged view at a in fig. 1;
fig. 5 is an enlarged view at B in fig. 1;
fig. 6 is a flowchart of a method for processing an isolated tumor tissue sample of oral cancer according to embodiment 2 of the present invention.
Reference numerals annotate: 1. a case; 2. cleaning the cavity; 3. a mounting plate; 4. a screw rod; 5. a guide rod; 6. a mounting base; 7. a partition plate; 8. a turntable; 9. a connecting rod; 10. a threaded rod; 11. a driving rod; 12. triggering a switch; 13. a self priming pump; 14. a spray head; 15. a mounting rod; 16. a liquid supply pipe; 17. a pipette; 18. a sealing strip; 19. a rotating shaft; 20. a slide; 21. a screen frame; 22. a first stepping motor; 23. a dip dyeing cavity; 24. a sector gear; 25. a rack; 26. an internal thread sleeve; 27. a drive pin; 28. a second stepping motor; 29. a vertical movable groove.
Detailed Description
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the description of the applications herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having" and any variations thereof in the description and claims of the present application and in the description of the figures above are intended to cover non-exclusive inclusions. The terms first, second and the like in the description and in the claims or in the above-described figures, are used for distinguishing between different objects and not necessarily for describing a sequential or chronological order.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
Example 1
The embodiment of the invention provides an in-vitro tumor tissue sample processing device for oral cancer, which is shown in fig. 1-5 and comprises:
the box body 1 is provided with a box door, can be installed through a hinge and a hasp, and can be symmetrically and uniformly provided with self-locking rollers at the bottom of the box body 1, so that the box is convenient to move;
the separation plate 7 is arranged in the box body 1 and is used for separating the inner cavity of the box body 1 into a dip dyeing cavity 23 and a cleaning cavity 2, a plurality of horizontal guide rods 5 are penetrated at the edge of the separation plate 7, the guide rods 5 are fixedly connected with the side wall of the box body 1 and can be welded and fixed, and it is to be explained that the dip dyeing cavity 23 is used for containing dip dyeing liquid which can be Lugol iodine liquid, the cleaning cavity 2 is used for containing cleaning liquid which can be PBS buffer liquid, of course, other prior art can be adopted, and no repeated description is needed, preferably, sealing strips 18 are arranged at the bottom, front and rear side walls of the separation plate 7, and a liquid inlet pipe and a liquid outlet pipe are respectively connected on the two side walls of the box body 1, and valves are respectively arranged on the liquid inlet pipe and the liquid outlet pipe, so that the dip dyeing liquid and the cleaning liquid can be conveniently added and discharged;
the installation plate 3 penetrating through the partition plate 7, the middle part of the installation plate 3 is rotationally connected with the partition plate 7 through a rotating shaft 19, two ends of the installation plate 3 are respectively positioned in the dip dyeing cavity 23 and the cleaning cavity 2, one end of the rotating shaft 19 extends to the outside of the box body 1 and is fixedly provided with a sector gear 24, a rack 25 is fixedly arranged on the outer wall of the box body 1 and can be welded and fixed, the rack 25 is positioned at the lower side of the sector gear 24 and is meshed with the sector gear 24, a strip groove for the rotating shaft 19 to move is formed in the outer wall of the box body 1, a flexible or elastic layer is arranged at the outer side of the strip groove, two ends of the flexible or elastic layer are adhered with the box body, the rotating shaft 19 penetrates through the flexible or elastic layer, the flexible or elastic layer can not interfere with the movement of the strip groove when the rotating shaft 19 moves, and can seal the strip groove, and reduce the communication with the outside, of course, other modes can be adopted, such as sliding installation of a sealing plate at the outer side of the strip groove, the rotating shaft 19 penetrates, the sealing plate moves when the sealing plate moves, and the sealing plate is long, and the sealing plate is always sealed;
the screen frame 21 is slidably arranged on the lower surface of the mounting plate 3 and is used for placing tumor tissue specimens, the partition plate 7 is provided with a through hole for the screen frame 21 to pass through, the top of the screen frame 21 can be hinged with the slide seat 20, the slide seat 20 is slidably connected with a slide groove formed on the lower surface of the mounting plate 3, two ends of the slide groove are closed, the screen frame 21 is formed by detachably encircling two half frames, one ends of the two half frames can be rotationally connected, the other ends of the two half frames are connected through a buckle, the screen frame 21 can be spherical, of course, other shapes can be adopted, and excessive limitation is not carried out;
the moving mechanism is arranged on the box body 1 and used for driving the partition plate 7 to move horizontally;
initially, as shown in fig. 1, the partition plate 7 is located at the middle position of the inner cavity of the box body 1, the box door is opened, a tumor tissue specimen is placed in the screen frame 21, the partition plate 7 is driven by the moving mechanism to move towards one side of the dip dyeing cavity 23, the liquid level in the dip dyeing cavity 23 rises, meanwhile, the sector gear 24 and the rack 25 move relatively, so that the sector gear 24 rotates anticlockwise, the sector gear 24 drives the mounting plate 3 to rotate anticlockwise through the rotating shaft 19, one end of the mounting plate 3 located in the dip dyeing cavity 23 and the screen frame 21 move downwards, the tumor tissue specimen enters the dip dyeing liquid, after the dip dyeing treatment is finished, the partition plate 7 is driven by the moving mechanism to move towards one side of the cleaning cavity 2, the liquid level in the cleaning cavity 2 rises, meanwhile, the sector gear 24 rotates clockwise, the sector gear 24 drives the mounting plate 3 to rotate clockwise through the rotating shaft 19, one end of the mounting plate 3 located in the cleaning cavity 2 descends into the cleaning liquid, the screen frame 21 does not need to slide from the dyeing cavity 23 to the cleaning cavity 2 in the process of the rotation of the mounting plate 3, the cleaning liquid is cleaned, the tumor tissue is automatically completed, the transfer from the dip dyeing treatment to the cleaning cavity is carried out, the manual transfer of the dip dyeing treatment is carried out, the work efficiency is reduced, the dip dyeing treatment is reduced, the mutual influence is reduced, the dip dyeing treatment efficiency is lowered, and the work is lowered, and the cleaning efficiency is lowered, and the mutually, and the between the cleaning treatment and the cleaning treatment is done.
Wherein, the mobile mechanism includes:
the screw rod 4 is horizontally rotatably arranged at the top of the box body 1 and can be rotatably arranged through a bearing;
the first stepping motor 22 is fixed on the box body 1 and is used for driving the screw rod 4 to rotate, the screw rod 4 can be fixed through a bolt, the first stepping motor 22 is a forward and reverse rotating motor, and an output shaft of the first stepping motor 22 can be fixedly connected with one end of the screw rod 4 through a coupler;
the installation seat 6 is in threaded connection with the screw rod 4, the installation seat 6 is in driving connection with the partition plate 7, and the installation seat 6 can be in sliding connection with the top of the box body 1;
it can be understood that the first stepper motor 22 is started, the first stepper motor 22 drives the screw rod 4 to rotate, the screw rod 4 drives the mounting seat 6 to move, and the mounting seat 6 drives the partition plate 7 to move;
specifically, the mounting seat 6 is connected with the partition plate 7 through a driving mechanism, and the driving mechanism comprises:
a second stepping motor 28 fixed to the mounting base 6, which can be fixed by bolts;
the turntable 8 fixed on the output shaft of the second stepping motor 28 can be fixed by welding;
the driving pin 27 is eccentrically arranged on the turntable 8, and the partition plate 7 is provided with a vertical movable groove 29 for the driving pin 27 to be inserted and movable;
the second stepping motor 28 is started, the second stepping motor 28 drives the turntable 8 to rotate, the turntable 8 drives the driving pin 27 to revolve, the driving pin 27 drives the partition plate 7 to reciprocate left and right, the partition plate 7 pushes the liquid in the dip dyeing cavity 23 and the cleaning cavity 2 to continuously flow, thereby continuously impacting the tumor tissue specimens in the net frame 21, being beneficial to improving dip dyeing and cleaning efficiency, meanwhile, the partition plate 7 drives the mounting plate 3 and the net frame 21 on the mounting plate to reciprocate left and right, and under the cooperation of the sector gear 24 and the rack 25, the mounting plate 3 reciprocates to drive the net frame 21 to reciprocate, the tumor tissue specimens in the net frame 21 continuously move in the liquid, further improving dip dyeing and cleaning efficiency, and meeting the requirement that the liquid can not flow to the other side through the through holes and not excessively limiting.
In this embodiment, the rotary table 8 is rotatably provided with a threaded rod 10, the threaded rod 10 is radially disposed along the rotary table 8, the threaded rod 10 is in threaded connection with an internal thread sleeve 26, the internal thread sleeve 26 is slidably connected with the rotary table 8, the driving pin 27 is fixed on the internal thread sleeve 26, a welding fixing manner can be adopted, by rotating the threaded rod 10, the threaded rod 10 drives the internal thread sleeve 26 to radially move along the rotary table 8, the internal thread sleeve 26 drives the driving pin 27 to move to adjust the position of the driving pin on the rotary table 8, the movement range of the driving pin is adjusted, and then the reciprocating movement range of the separation plate 7 is adjusted, so as to adjust the liquid flow effect;
in a specific implementation, a plurality of ejection pieces are installed on two side walls of the partition plate 7 from top to bottom at intervals, each ejection piece comprises a mounting rod 15 and a spray head 14, one end of each mounting rod 15 is hinged to the side wall of the partition plate 7, the other end of each mounting rod 15 is provided with the spray head 14 for spraying liquid to the screen frame 21, the spray heads 14 are installed through screws, a liquid supply assembly is arranged in the box body 1 for supplying liquid to the spray heads 14, and preferably, a power mechanism for driving the mounting rods 15 to swing up and down is arranged in the box body 1 and comprises:
a connecting rod 9 rotatably mounted on the driving pin 27;
the driving rods 11 fixed at the two ends of the connecting rod 9 can be fixed through welding, the two driving rods 11 are respectively matched and connected with the mounting rods 15 on the two side walls of the partition plate 7, the driving rods 11 are vertically arranged and are in sliding connection with the side walls of the partition plate 7, a plurality of pin shafts corresponding to the mounting rods 15 one by one are fixed on the driving rods 11 in a sliding connection mode of sliding blocks and sliding grooves, and through grooves for the pin shafts to be inserted and move are formed in the mounting rods 15;
it can be understood that when the turntable 8 drives the driving pin 27 to rotate, the driving pin 27 drives the driving rod 11 to move up and down through the connecting rod 9, the driving rod 11 drives the mounting rod 15 to swing up and down through the pin shaft, the mounting rod 15 drives the spray head 14 to swing up and down, and liquid can be sprayed out in a multi-angle and large range, so that the liquid is fully contacted and impacted with the tumor tissue specimen in the screen frame 21, and the dip dyeing and cleaning effects are improved;
in this embodiment, the liquid supply assembly includes:
the self-priming pump 13 which is fixedly arranged on the partition plate and positioned below the mounting plate 3 can be arranged through bolts;
two liquid suction pipes 17 connected to the inlet of the self-priming pump 13, wherein the two liquid suction pipes 17 extend to the lower parts of the dip dyeing cavity 23 and the cleaning cavity 2 respectively, and electromagnetic valves are respectively arranged on the two liquid suction pipes 17;
the two liquid supply pipes 16 are connected to the outlet of the self-priming pump 13, the two liquid supply pipes 16 are respectively communicated with the dip dyeing cavity 23 and the spray head 14 in the cleaning cavity 2, and electromagnetic valves are respectively arranged on the two liquid supply pipes 16;
the two trigger switches 12 are symmetrically arranged on the partition plate 7 and positioned at the lower side of the mounting plate 3, in the embodiment, the trigger switches 12 are L-shaped, the bottom ends of the two trigger switches 12 are rotationally connected with the partition plate 7, a reset torsion spring is connected between the two trigger switches 12 and the partition plate 7, the top ends of the two trigger switches 12 extend to the lower side of the partition plate 7 towards two sides, preferably, the distance between the top ends of the trigger switches 12 and the lower surface of the partition plate 7 is 1cm, the two trigger switches 12 are respectively electrically connected with the self-priming pump 13, the start and stop of the self-priming pump 13 can be controlled, and each trigger switch 12 is electrically connected with a liquid suction pipe 17 at the same side and an electromagnetic valve on the liquid supply pipe 16, and the opening and closing of the electromagnetic valve can be controlled;
it should be explained that, when the left end of the mounting plate 3 rotates downwards to enter the dip-dyeing cavity 23, the mounting plate 3 pushes the left trigger switch 12 to push the self-priming pump 13 to rotate, meanwhile, the solenoid valve on the liquid suction pipe 17 and the liquid supply pipe 16 in the dip-dyeing cavity 23 is opened, the self-priming pump 13 sucks the dip-dyeing liquid from the liquid suction pipe 17 and the liquid supply pipe 16 in the dip-dyeing cavity 23, then the dip-dyeing liquid is pumped into the liquid supply pipe 16 in the dip-dyeing cavity 23, the spray head 14 in the dip-dyeing cavity 23 sprays the dip-dyeing liquid, after the dip-dyeing treatment is finished, the mounting plate 3 rotates to reset and is separated from the left trigger switch 12, the self-priming pump 13 stops, the liquid suction pipe 17 and the solenoid valve on the liquid supply pipe 16 in the dip-dyeing cavity 23 are closed, then the right end of the mounting plate 3 rotates downwards to enter the cleaning cavity 2, the self-priming pump 12 pushes the self-priming pump 13 to rotate, the self-priming pump 12 is controlled to start, meanwhile, the solenoid valve on the liquid suction pipe 17 and the liquid supply pipe 16 in the cleaning cavity 2 is opened, the self-priming pump 13 is pumped into the liquid supply pipe 16 from the liquid suction pipe 17 in the cleaning cavity 2, the liquid suction pipe 17 in the cleaning cavity 2 is reset and the spray head 14 in the cleaning cavity 2 is driven to the cleaning liquid in the reset cavity 2, and the cleaning mechanism is driven to be in a horizontal state, and the cleaning liquid is washed by the spray head mechanism is driven to be in the cleaning cavity 3, and after the cleaning mechanism is in the cleaning cavity 2 and the cleaning cavity and has been cleaned.
In summary, the embodiment of the invention provides an oral cancer in-vitro tumor tissue sample processing device, which has the following working principle:
opening a box door, placing a tumor tissue specimen into a screen frame 21, driving a partition plate 7 to move towards one side of a dip dyeing cavity 23 through a moving mechanism, enabling the liquid level in the dip dyeing cavity 23 to rise, enabling a sector gear 24 and a rack 25 to move relatively, enabling the sector gear 24 to rotate anticlockwise, enabling the sector gear 24 to drive a mounting plate 3 to rotate anticlockwise through a rotating shaft 19, enabling one end of the mounting plate 3 positioned in the dip dyeing cavity 23 and the screen frame 21 to move downwards, entering into dip dyeing liquid, enabling the mounting plate 3 to press a left trigger switch 12 to push the left trigger switch to rotate, enabling the left trigger switch 12 to control a self-priming pump 13 to start, enabling a liquid suction pipe 17 in the dip dyeing cavity 23 and an electromagnetic valve on a liquid supply pipe 16 to open, enabling the self-priming pump 13 to suck the dip dyeing liquid from the liquid suction pipe 17 in the dip dyeing cavity 23, enabling the dip dyeing liquid to be pumped into the liquid supply pipe 16 in the dip dyeing cavity 23, enabling a spray head 14 in the dip dyeing cavity 23 to spray the dyeing liquid, after the dip dyeing treatment is finished, the partition plate 7 is driven to move to one side of the cleaning cavity 2 by the moving mechanism, the liquid level in the cleaning cavity 2 rises, meanwhile, the sector gear 24 rotates clockwise, the sector gear 24 drives the mounting plate 3 to rotate clockwise through the rotating shaft 19, one end of the mounting plate 3 positioned in the cleaning cavity 2 descends into cleaning liquid, during the rotation process of the mounting plate 3, the net frame 21 slides from the dip dyeing cavity 23 into the cleaning cavity 2 along the mounting plate 3 and enters the cleaning liquid, meanwhile, the mounting plate 3 presses down the right trigger switch 12 to push the right trigger switch 12 to rotate, the right trigger switch 12 controls the self-sucking pump 13 to start, simultaneously, the liquid suction pipe 17 in the cleaning cavity 2 and the electromagnetic valve on the liquid supply pipe 16 are opened, the self-sucking pump 13 sucks the cleaning liquid from the liquid suction pipe 17 in the cleaning cavity 2, then pumps the cleaning liquid into the liquid supply pipe 16 in the cleaning cavity 2, so that the spray head 14 in the cleaning cavity 2 sprays the cleaning liquid, after the cleaning is completed, the moving mechanism drives the partition plate 7 to reset, the mounting plate 3 is reset to a horizontal state, the box door is opened, and the tumor tissue specimen in the net frame 21 is taken out for detection.
Example 2
The embodiment of the invention provides a treatment method based on the oral cancer in-vitro tumor tissue sample treatment device in the embodiment 1, as shown in fig. 6, comprising the following steps:
an isolated tumor tissue specimen (primary focus specimen and lymph node specimen) of the oral cancer is obtained and placed in a screen frame 21;
adding a dip dyeing liquid into the dip dyeing cavity 23, and adding a cleaning liquid into the cleaning cavity 2;
the partition plate 7 is driven to move to one side of the dip dyeing cavity 23 through the moving mechanism, meanwhile, the sector gear 24 and the rack 25 move relatively, the sector gear 24 rotates anticlockwise, the sector gear 24 drives the mounting plate 3 to rotate anticlockwise through the rotating shaft 19, one end of the mounting plate 3 positioned in the dip dyeing cavity 23 and the screen frame 21 move downwards, enter the dip dyeing liquid and dip dye for 1-2h;
after dip dyeing treatment, the partition plate 7 is driven to move to one side of the cleaning cavity 2 by the moving mechanism, meanwhile, the sector gear 24 rotates clockwise, the sector gear 24 drives the mounting plate 3 to rotate clockwise by the rotating shaft 19, one end of the mounting plate 3 positioned in the cleaning cavity 2 descends into cleaning liquid, and in the rotating process of the mounting plate 3, the net frame 21 slides along the mounting plate 3 from the dip dyeing cavity 23 into the cleaning cavity 2 and enters into the cleaning liquid for cleaning for 2-4min;
after the cleaning treatment, slicing and bleaching a tumor tissue specimen to obtain a specimen slice;
the image scanning and collecting unit is used for collecting images of the specimen slices and carrying out pathological section examination and immunohistochemical examination, and particularly, the image scanning and collecting unit can adopt equipment in the prior art.
It should be noted that, for simplicity of description, the foregoing embodiments are all illustrated as a series of acts, but it should be understood by those skilled in the art that the present invention is not limited by the order of acts, as some steps may be performed in other order or concurrently in accordance with the present invention. Further, those skilled in the art will also appreciate that the embodiments described in the specification are all preferred embodiments, and that the acts and modules referred to are not necessarily required for the present invention.
The above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention. It will be apparent that the described embodiments are merely some, but not all, embodiments of the invention. Based on these embodiments, all other embodiments that may be obtained by one of ordinary skill in the art without inventive effort are within the scope of the invention.
Claims (10)
1. An oral cancer in vitro tumor tissue sample processing device, comprising:
a case;
the separation plate is arranged in the box body and is used for separating the inner cavity of the box body into a dip dyeing cavity and a cleaning cavity, a plurality of horizontal guide rods are arranged on the edge of the separation plate in a penetrating manner, and the guide rods are fixedly connected with the side wall of the box body;
the mounting plate penetrates through the partition plate, the middle part of the mounting plate is rotationally connected with the partition plate through a rotating shaft, two ends of the mounting plate are respectively positioned in the dip dyeing cavity and the cleaning cavity, one end of the rotating shaft extends to the outside of the box body and is fixedly provided with a sector gear, a rack is fixed on the outer wall of the box body, the rack is positioned at the lower side of the sector gear and meshed with the sector gear, and a strip groove for the rotation shaft to move is formed in the outer wall of the box body;
the screen frame is slidably arranged on the lower surface of the mounting plate and used for placing tumor tissue specimens, and the partition plate is provided with through holes for the screen frame to pass through;
and the moving mechanism is arranged on the box body and used for driving the partition plate to move horizontally.
2. The device for processing the oral cancer in-vitro tumor tissue specimens according to claim 1, wherein sealing strips are arranged on the bottom, the front side wall and the rear side wall of the partition plate, a liquid inlet pipe and a liquid outlet pipe are respectively connected to the two side walls of the box body, and valves are arranged on the liquid inlet pipe and the liquid outlet pipe.
3. The device for processing the tissue specimens of the in-vitro tumor of the oral cancer according to claim 1, wherein the top of the net frame is hinged with a sliding seat, the sliding seat is in sliding connection with a sliding groove formed in the lower surface of the mounting plate, and two ends of the sliding groove are closed.
4. The oral cancer in-vitro tumor tissue specimen processing device according to claim 1, wherein the screen frame is formed by detachably encircling two half frames, one ends of the two half frames are rotationally connected, and the other ends of the two half frames are connected through buckles.
5. The oral cancer ex vivo tumor tissue sample processing device of claim 1, wherein the movement mechanism comprises:
the screw rod is horizontally rotatably arranged at the top of the box body;
the first stepping motor is fixed on the box body and is used for driving the screw rod to rotate;
the mounting seat is in threaded connection with the screw rod, the mounting seat is in driving connection with the partition plate, and the mounting seat is in sliding connection with the top of the box body.
6. The oral cancer in vitro tumor tissue sample processing device according to claim 5, wherein the mounting base and the partition plate are connected by a driving mechanism, the driving mechanism comprising:
the second stepping motor is fixed on the mounting seat;
a turntable fixed on the output shaft of the second stepping motor;
the driving pin is eccentrically arranged on the turntable, and the vertical movable groove for the driving pin to be inserted and move is formed in the partition plate.
7. The oral cancer in-vitro tumor tissue sample processing device according to claim 6, wherein a threaded rod is rotatably mounted on the turntable, the threaded rod is radially arranged along the turntable, an internal thread sleeve is in threaded connection with the threaded rod, the internal thread sleeve is in sliding connection with the turntable, and the driving pin is fixed on the internal thread sleeve.
8. The device for processing the tissue specimens of the in-vitro tumor of the oral cancer according to claim 6, wherein a plurality of spraying pieces are arranged on two side walls of the partition plate from top to bottom at intervals, each spraying piece comprises a mounting rod and a spray head, one end of each mounting rod is hinged with the side wall of the partition plate, the spray head is arranged at the other end of each mounting rod and used for spraying liquid to the screen frame, a liquid supply assembly is arranged in the box body and used for supplying liquid to the spray head, and a power mechanism used for driving the mounting rod to swing up and down is arranged in the box body and comprises:
a connecting rod rotatably mounted on the drive pin;
the driving rods are fixed at two ends of the connecting rod, the two driving rods are respectively connected with the mounting rods on two side walls of the partition plate in a matched mode, the driving rods are vertically arranged and are in sliding connection with the side walls of the partition plate, a plurality of pin shafts which are in one-to-one correspondence with the mounting rods are fixed on the driving rods, and through grooves for the pin shafts to be inserted and move are formed in the mounting rods.
9. The oral cancer ex vivo tumor tissue sample processing device of claim 8, wherein the fluid supply assembly comprises:
the self-priming pump is fixedly arranged on the partition plate and positioned below the mounting plate;
two liquid suction pipes connected to the inlet of the self-priming pump, wherein the two liquid suction pipes extend to the lower parts of the dip dyeing cavity and the cleaning cavity respectively, and electromagnetic valves are arranged on the two liquid suction pipes respectively;
two liquid supply pipes connected to the outlet of the self-priming pump, wherein the two liquid supply pipes are respectively communicated with the spray heads in the dip dyeing cavity and the cleaning cavity, and electromagnetic valves are respectively arranged on the two liquid supply pipes;
the left and right sides symmetry is installed on the division board and is located two trigger switch of mounting panel downside, two trigger switch's bottom is connected with the division board rotation and is connected with reset torsion spring between the division board, two trigger switch's top extends to the division board downside to both sides, and two trigger switch are connected with the self priming pump electricity respectively, can control the start-stop of self priming pump, and every trigger switch is connected with the solenoid valve electricity on the pipette and the feed pipe of homonymy, can control the switching of solenoid valve.
10. A method for processing an oral cancer in vitro tumor tissue sample processing device according to any one of claims 1 to 9, comprising the steps of:
obtaining an in-vitro tumor tissue sample of the oral cancer, and placing the in-vitro tumor tissue sample in a net frame;
adding a dip dyeing liquid into the dip dyeing cavity, and adding a cleaning liquid into the cleaning cavity;
the partition plate is driven to move to one side of the dip dyeing cavity by the moving mechanism, the sector gear and the rack move relatively, the sector gear rotates anticlockwise, the sector gear drives the mounting plate to rotate anticlockwise by the rotating shaft, one end of the mounting plate positioned in the dip dyeing cavity and the net frame move downwards, and the mounting plate enters into dip dyeing liquid to be dip dyed for 1-2h;
after dip dyeing treatment, the partition plate is driven to move to one side of the cleaning cavity by the moving mechanism, meanwhile, the sector gear rotates clockwise, the sector gear drives the mounting plate to rotate clockwise by the rotating shaft, one end of the mounting plate, which is positioned in the cleaning cavity, descends into the cleaning liquid, and in the rotating process of the mounting plate, the screen frame slides along the mounting plate from the dip dyeing cavity into the cleaning cavity, enters into the cleaning liquid and is cleaned for 2-4min;
after the cleaning treatment, slicing and bleaching a tumor tissue specimen to obtain a specimen slice;
and acquiring images of the specimen slices through an image scanning acquisition unit, and performing pathological section examination and immunohistochemical examination.
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| CN202311681966.1A CN117367923B (en) | 2023-12-08 | 2023-12-08 | Device and method for processing oral cancer in-vitro tumor tissue specimens |
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1408320A2 (en) * | 2002-10-09 | 2004-04-14 | S.A. Francisco Soria Melguizo | Programmable automatic system for microbiological, histological and cytological stainings |
| US6899848B1 (en) * | 2001-02-27 | 2005-05-31 | Hamilton Company | Automated sample treatment system: apparatus and method |
| CN207222426U (en) * | 2017-04-21 | 2018-04-13 | 江苏东宇墙纸有限公司 | A kind of cleaning device |
| WO2018205691A1 (en) * | 2017-05-09 | 2018-11-15 | 南昌德漫多科技有限公司 | System for processing histopathological specimen |
| CN212379142U (en) * | 2020-06-16 | 2021-01-19 | 湖北欣立达科技有限公司 | Cell staining machine with staining slide washes function |
| CN112323306A (en) * | 2020-11-23 | 2021-02-05 | 邓倩 | Textile cloth dip-dyeing process |
| CN112893253A (en) * | 2021-01-18 | 2021-06-04 | 丁亚宁 | Medical instrument cleaning and drying equipment for surgical operation |
| CN114152495A (en) * | 2021-11-19 | 2022-03-08 | 安徽医科大学第一附属医院 | Combined device for early diagnosis of oral cancer by fluorescent dye enrichment |
| CN114279798A (en) * | 2021-04-25 | 2022-04-05 | 福州安林生物科技有限公司 | Full-automatic immunohistochemical staining instrument and staining method thereof |
| CN114717769A (en) * | 2022-03-24 | 2022-07-08 | 江苏臻善纺织品有限公司 | Dyed yarn dip-dyeing equipment with carding structure and dip-dyeing method thereof |
| CN217466409U (en) * | 2022-01-10 | 2022-09-20 | 上海羿岑生物科技有限公司 | Liver disease sample check out test set |
| KR20230050917A (en) * | 2021-10-08 | 2023-04-17 | 서울대학교병원 | Kit for staining tissue for on-site evaluation |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2877855T3 (en) * | 2013-12-13 | 2021-11-17 | Ventana Med Syst Inc | Staining reagents and other liquids for histological processing of biological samples and associated technology |
-
2023
- 2023-12-08 CN CN202311681966.1A patent/CN117367923B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6899848B1 (en) * | 2001-02-27 | 2005-05-31 | Hamilton Company | Automated sample treatment system: apparatus and method |
| EP1408320A2 (en) * | 2002-10-09 | 2004-04-14 | S.A. Francisco Soria Melguizo | Programmable automatic system for microbiological, histological and cytological stainings |
| CN207222426U (en) * | 2017-04-21 | 2018-04-13 | 江苏东宇墙纸有限公司 | A kind of cleaning device |
| WO2018205691A1 (en) * | 2017-05-09 | 2018-11-15 | 南昌德漫多科技有限公司 | System for processing histopathological specimen |
| CN212379142U (en) * | 2020-06-16 | 2021-01-19 | 湖北欣立达科技有限公司 | Cell staining machine with staining slide washes function |
| CN112323306A (en) * | 2020-11-23 | 2021-02-05 | 邓倩 | Textile cloth dip-dyeing process |
| CN112893253A (en) * | 2021-01-18 | 2021-06-04 | 丁亚宁 | Medical instrument cleaning and drying equipment for surgical operation |
| CN114279798A (en) * | 2021-04-25 | 2022-04-05 | 福州安林生物科技有限公司 | Full-automatic immunohistochemical staining instrument and staining method thereof |
| KR20230050917A (en) * | 2021-10-08 | 2023-04-17 | 서울대학교병원 | Kit for staining tissue for on-site evaluation |
| CN114152495A (en) * | 2021-11-19 | 2022-03-08 | 安徽医科大学第一附属医院 | Combined device for early diagnosis of oral cancer by fluorescent dye enrichment |
| CN217466409U (en) * | 2022-01-10 | 2022-09-20 | 上海羿岑生物科技有限公司 | Liver disease sample check out test set |
| CN114717769A (en) * | 2022-03-24 | 2022-07-08 | 江苏臻善纺织品有限公司 | Dyed yarn dip-dyeing equipment with carding structure and dip-dyeing method thereof |
Non-Patent Citations (2)
| Title |
|---|
| Tiziana Tenuta等.Elution of Labile Fluorescent Dye from Nanoparticles during Biological Use.2011,第6卷(第10期),1-6. * |
| 涤棉分散染料微胶囊/活性染料一浴法染色;王晓文;罗艳;杜鹃;陈水林;;印染;20060930(第18期);17-19 * |
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|---|---|
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