CN220542458U

CN220542458U - Pathological section machine

Info

Publication number: CN220542458U
Application number: CN202321560499.2U
Authority: CN
Inventors: 黄爱本; 杨毅斌; 黄宗涌; 黄燚彬; 黄斐一
Original assignee: Xiamen Maternal And Child Health Hospital Xiamen Eugenics Service Center Xiamen University Affiliated Women's And Children's Hospital Xiamen Linqiaozhi Women's And Children's Hospital
Current assignee: Xiamen Maternal And Child Health Hospital Xiamen Eugenics Service Center Xiamen University Affiliated Women's And Children's Hospital Xiamen Linqiaozhi Women's And Children's Hospital
Priority date: 2023-06-19
Filing date: 2023-06-19
Publication date: 2024-02-27
Anticipated expiration: 2033-06-19

Abstract

The utility model relates to the technical field of medical equipment, in particular to a pathological section machine. The slicer includes a slicer body including a slicing mechanism for performing a slicing operation; the transmission mechanism is provided with a receiving end and a transmission end opposite to the receiving end, and the receiving end is positioned below the slicing mechanism so as to transmit the sliced tissue sample to the transmission end; a collection trough positioned below the transmission end to collect the tissue sample conveyed by the transmission mechanism, and comprising a first trough wall formed by surrounding and a first trough bottom positioned at the bottom of the first trough wall; the water receiving tank comprises a second tank wall and a second tank bottom, the second tank wall is arranged on the outer wall of the first tank wall of the water collecting tank in a surrounding mode, the second tank bottom is positioned at the bottom of the second tank wall, and the top surface of the first tank wall is higher than the top surface of the second tank wall; the top of the first groove wall is provided with a notch, and the notch is provided with a flow speed regulating and controlling component which is used for controlling the flow speed of water flowing into the receiving groove from the collecting groove. The slicer provided by the utility model is convenient to operate and high in working efficiency.

Description

Pathological section machine

Technical Field

The utility model relates to the technical field of medical equipment, in particular to a pathological section machine.

Background

Tissue preparation is a common method for observing and researching normal morphology and pathological changes of tissues and cells in the disciplines of histology, embryology, pathology, biology and the like. The basic principle is to fix tissues, cells and various subcellular organelles with fixatives (usually paraffin), keep the microstructure of the tissues, make slices, increase the chromatic aberration of each part by different staining methods, observe the morphological structure of the tissues and cells under a microscope, or display certain chemical components in the tissues and cells by chemical or physical methods, and perform quantitative analysis of the morphology and chemical components.

Paraffin is insoluble in water and soluble in organic solvents such as xylene, so that the fixed tissue is dehydrated and transparent by removing water from the tissue with dehydrating agents such as ethanol, acetone, n-butanol, and the like, and then replacing ethanol with a transparent agent such as xylene. And then the paraffin is used for penetrating into the tissue block, and the tissue block is hardened after condensation, namely, the tissue block is immersed in the paraffin and embedded, so that the tissue block can be sliced on a slicing machine.

Tissue tableting typically includes the following steps:

1. fixing

After the cells and tissues are isolated, the cells and tissues are quickly fixed by the corresponding fixative, so that proteins are precipitated, denatured and coagulated, and the original morphological structure and antigenicity of the tissues are maintained. Paraformaldehyde, FAA, acetone or Carnoy are typically used and allowed to set overnight at 4 ℃.

2. Dehydration and transparency

The fixed tissue must be dehydrated stepwise from low to high concentration ethanol, typically 50%, 70%, 85%, 95%, and 100% (3 times).

3. Wax infiltration and embedding

Paraffin penetration is the process of replacing the transparentizing agent in the tissue mass with paraffin generally having a melting point of 52-60 ℃. The transparent tissue block is infiltrated overnight at 37 ℃ in 1/1 volume of xylene and paraffin, then the temperature is adjusted to 58 ℃ and the pure paraffin is replaced for continuous infiltration, and the pure paraffin is replaced at intervals of 3 hours generally until no xylene exists.

4. Slicing and expanding sheet

Excess paraffin around the tissue block is trimmed by a blade, and the paraffin with the length of about 2mm is generally reserved and trimmed into a trapezoid tissue block, so that the paraffin tape can be separated during the sheet spreading. The blade is heated firstly, then the trimmed tissue block is quickly adhered to a square small wood block, and slicing can be performed after condensation.

The thickness of the tissue slice is 6-12um, the slice speed is preferably 40-50 times/min, a wax belt is supported by a writing brush, the wax belt is orderly placed on a clean glass slide after being divided, the smooth surface of the wax belt is placed downwards, and then a proper amount of distilled water is dripped on a slice spreading table to bake slices at 37-40 ℃ overnight.

5. Dyeing, dewatering and sealing sheet

Staining is generally double or single, safranin and fast green are common double staining methods, while hematoxylin is the most excellent nuclear stain. After dyeing, the required tissue slice is obtained after dehydration and slice sealing treatment.

After one-time slicing is completed, the sliced tissue sample is required to be spread, and the sample is easily polluted and wasted due to falling in the process of transferring the tissue sample on the slicing machine to the tissue spreading machine; in addition, after one-time sheet spreading is completed in the tissue sheet spreading machine, a large amount of tissue cells, paraffin and other pollutants are often floated on the surface of the aqueous solution in the tissue sheet spreading machine, and the sheet spreading water tank is exposed to the air for a long time because of frequent use, so that dust in the air is easy to fall on the water surface, and the sheet spreading operation is influenced. Before the next spreading, the pollutants and dust must be cleaned up to avoid polluting the subsequent spreading.

Referring to fig. 1, a conventional method for cleaning contaminants floating on the surface 1 of an aqueous solution in a microtome is shown. At present, wax sheets floating on the liquid level (shown by a dotted line in fig. 1) are cleaned, and mainly, water absorbing paper (shown by the dotted line in fig. 1) is used for scraping or absorbing floating matters on the liquid level. According to the cleaning method, the absorbent paper is easy to break, so that the absorbent paper is not cleaned cleanly, is very difficult to control, and has low working efficiency. The present utility model has been made in view of such a background.

Disclosure of Invention

The utility model aims to overcome the defects in the prior art and provide a pathological section machine.

In order to solve the technical problems, the technical scheme provided by the utility model is as follows:

a pathological microtome, comprising:

a microtome body including a slicing mechanism for performing a slicing operation;

a transport mechanism having a receiving end and a transport end opposite the receiving end, the receiving end of the transport mechanism being positioned below the sectioning mechanism for transporting sectioned tissue samples to the transport end;

the collecting water tank is positioned below the transmission end and used for collecting the tissue samples conveyed by the transmission mechanism, and comprises a first tank wall formed by surrounding and a first tank bottom positioned at the bottom of the first tank wall;

the water collecting tank comprises a water collecting tank and a water collecting tank, wherein the water collecting tank comprises a second tank wall and a second tank bottom, the second tank wall is arranged on the outer wall of a first tank wall of the water collecting tank in a surrounding mode, the second tank bottom is arranged at the bottom of the second tank wall, and the top surface of the first tank wall is higher than the top surface of the second tank wall;

the top of the first groove wall is provided with a notch, the notch is provided with a flow speed regulating and controlling component, and the flow speed regulating and controlling component is used for controlling the flow speed of water flowing into the water receiving groove from the water collecting groove.

In a more preferred embodiment, a buffer water tank is arranged on the first tank wall, so that water in the collecting water tank flows into the buffer water tank and the receiving water tank sequentially through the notch.

In a more preferred embodiment, the flow rate regulating assembly comprises a slide rail arranged in the notch and two water baffles which are respectively arranged along the slide rail in a reciprocating sliding way from two ends to the center.

In a preferred embodiment, the splash guard has an operating lever thereon that projects outwardly toward the splash guard surface.

In a more preferred embodiment, the buffer tank includes a water outlet section tank wall, a top surface of the water outlet section tank wall being lower than a top surface of the first tank wall, and a top surface of the water outlet section tank wall being higher than a top surface of the second tank wall.

In a further preferred embodiment, the pathological microtome further comprises: the circulating assembly is arranged at the bottoms of the collecting water tank and the receiving water tank;

the circulating assembly is respectively communicated with the collecting water tank and the water receiving tank, and water in the water receiving tank is sent into the collecting water tank from the bottom of the collecting water tank.

In a preferred embodiment, the second tank bottom is provided with a plurality of openings so that water flowing from the collecting tank to the receiving tank flows into the circulating assembly through the openings.

In a more preferred embodiment, a filter assembly is disposed within the circulation assembly for filtering water flowing through the circulation assembly.

In a more preferred embodiment, the height of the receiving end is higher than the height of the transmitting end.

In summary, the present application includes at least one of the following beneficial technical effects:

the pathological section machine provided by the utility model is convenient to operate and high in working efficiency.

Additional features and advantages of the utility model will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model. The objectives and other advantages of the utility model will be realized and attained by the structure particularly pointed out in the written description and claims hereof as well as the appended drawings.

Drawings

For a clearer description of embodiments of the utility model or of the solutions of the prior art, the drawings that are needed in the description of the embodiments or of the prior art will be briefly described, it being obvious that the drawings in the description below are some embodiments of the utility model, and that other drawings can be obtained from them without inventive effort for a person skilled in the art; the positional relationships described in the drawings in the following description are based on the orientation of the elements shown in the drawings unless otherwise specified.

FIG. 1 is a schematic diagram of a conventional pathological microtome;

fig. 2 is a schematic structural diagram of an ash removal sheet-spreading machine according to an embodiment of the present utility model;

FIG. 3 is a schematic top view of an ash removal sheet spreader according to an embodiment of the present utility model;

FIG. 4 is a schematic view of a water tank structure according to an embodiment of the present utility model;

FIG. 5 is a schematic top view of a sink structure according to an embodiment of the present utility model;

FIG. 6 is a schematic view of another view angle structure of a sink structure according to an embodiment of the present utility model;

FIG. 7 is an enlarged schematic view of the structure within the circle of the embodiment shown in FIG. 6.

Reference numerals:

10. a slicer body; 11. a slicing mechanism; 20. a transmission mechanism; 21. a receiving end; 22. a transmission end; 30. a collecting tank; 31. a first groove wall; 32. a first groove bottom; 32a, a water outlet; 33. a notch; 34. a flow rate regulation assembly; 341. a slideway; 342. a water baffle; 343. an operation lever; 40. a buffer water tank; 41. a water outlet section groove wall; 50. a water receiving tank; 51. a second groove wall; 52. a second groove bottom; 52a, opening holes; 60. and (3) a circulating assembly.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more clear, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model; the technical features designed in the different embodiments of the utility model described below can be combined with each other as long as they do not conflict with each other; all other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

In the description of the present utility model, it should be noted that all terms used in the present utility model (including technical terms and scientific terms) have the same meaning as commonly understood by one of ordinary skill in the art to which the present utility model belongs and are not to be construed as limiting the present utility model; it will be further understood that terms used herein should be interpreted as having a meaning that is consistent with their meaning in the context of this specification and the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "provided," "connected," and the like are to be construed broadly, and may be fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

The technical scheme of the utility model is further illustrated and described through specific examples. The scope of the utility model is not limited in this respect. The different embodiments disclosed below may reuse the same reference symbols and/or labels. These repetition are for the purpose of simplicity and clarity and do not in itself dictate a particular relationship between the various embodiments and/or configurations discussed.

Referring to fig. 2-7, an embodiment of the present utility model provides a pathological microtome, comprising: a slicer body 10, a transfer mechanism 20, a collection trough 30, a buffer trough 40, a receiving trough 50, and a circulation assembly 60;

a slicer body 10, the slicer body 10 including a slicing mechanism 11 for performing a slicing operation;

a transport mechanism 20, the transport mechanism 20 having a receiving end 21 and a transport end 22 opposite the receiving end 21, the receiving end 21 of the transport mechanism 20 being positioned below the microtome 11 for transporting the sectioned tissue sample to the transport end 22, the transport mechanism 20 may be a conveyor belt or the like. Preferably, the receiving end 21 has a height greater than the height of the delivery end 22, which may facilitate downward transport of the tissue sample.

A collection trough 30, see fig. 4-7, wherein the collection trough 30 is located below the conveying end 22 for collecting the tissue samples conveyed by the conveying mechanism 20, and the collection trough 30 includes a first trough wall 31 formed by surrounding and a first trough bottom 32 located at the bottom of the first trough wall 31;

the top of the first tank wall 31 has a notch 33, the notch 33 is provided with a flow rate regulating component 34, the flow rate regulating component 34 is used for controlling the flow rate of water flowing from the collecting tank into the receiving tank, and the notch 33 on the top is provided with the notch 33 on the top of the first tank wall 31 based on the flow rate regulating component 34, so that the flow rate of water can be effectively regulated by the flow rate regulating component 34 on one hand, water in the collecting tank 30 is discharged, especially after the circulating component 60 is matched, water circulation in a pathological slicing machine can be realized, more importantly, a notch 33 is selectively formed on the top of the first tank wall 31, the notch 33 on the top is different from the notch 33 arranged on the first tank wall 31, and pollutants or useless wax sheets floating on the water can also flow out together and are not blocked, so that the pollutants or useless wax sheets needing to be scraped off or adsorbed by an operator by using water absorbing paper (shown by a dotted line in fig. 1) can be reduced, the water absorbing paper is broken easily, the water absorbing liquid level is difficult to clean, and the cleaning efficiency is difficult to control under the conditions. In a specific embodiment, the flow rate regulating assembly 34 includes a slide way 341 disposed in the notch, two water baffles 342 disposed along the slide way 341 and sliding reciprocally from two ends to the center, and the opening size of the top notch 33 can be regulated very conveniently by setting the water baffles 342, so as to control the flow rate of water flow; preferably, as shown in fig. 7, the dam 342 has an operation lever 343 formed to protrude outward toward the surface of the dam so as to facilitate adjustment of the position of the dam 342.

The buffer water tank 40 is arranged on the first tank wall 31, so that water in the collecting water tank 30 flows into the buffer water tank 40 and the receiving water tank 50 in sequence through the notch 33, and through the design of the buffer water tank 40, the situation that the notch 33 is opened too much due to improper operation, a large amount of water flows into the receiving water tank 50 from the collecting water tank 30, so that water flow splashes and the like are caused, and the environmental cleaning of surrounding experiment tables is effectively kept; the buffer water tank 40 includes a water outlet section tank wall 41, the top surface of the water outlet section tank wall 41 is lower than the top surface of the first tank wall 31, and the top surface of the water outlet section tank wall 41 is higher than the top surface of the second tank wall 41, so that a stepped water tank structure is formed, water in the collecting water tank 30 can more conveniently flow into the buffer water tank 40 via the notch 33, and the buffer water tank 40 and the receiving water tank 50 have the same height difference, so that water flows in each stage have a certain buffer flow space.

The water receiving tank 50, the water receiving tank 50 includes a second tank wall 51 surrounding the outer wall of the first tank wall 31 of the water collecting tank 30 and a second tank bottom 52 located at the bottom of the second tank wall 51, the top surface of the first tank wall 31 is higher than the top surface of the second tank wall 51, and by the design of the water receiving tank 50, water flowing out of the water collecting tank 50 can be effectively guaranteed not to flow onto the operation table top.

In a preferred embodiment, the pathological microtome further comprises: a circulation assembly 60 provided at the bottom of the collecting tank 30 and the receiving tank 50; specifically, the circulation assembly 60 is respectively connected to the collecting tank 30 and the receiving tank 50, and sends the water in the receiving tank 50 into the collecting tank 30 from the bottom of the collecting tank 30, as shown in fig. 5, the first tank bottom 32 is provided with a water outlet 32a, and the circulation assembly 60 may have a structure known to those skilled in the art, such as a water pump, etc., for sending the water in the receiving tank 50 into the collecting tank 30.

Preferably, the second tank bottom 52 is provided with a plurality of openings 52a, so that the water flowing from the collecting tank 30 to the receiving tank 50 flows into the circulating assembly 60 through the openings 52a, and the water flowing from the receiving tank 50 into the circulating assembly 60 can be dispersed more smoothly and uniformly by the design of the plurality of openings 52 a.

Preferably, a filtering component is disposed in the circulating component 60, so as to filter the water flowing through the circulating component 60, further ensure the cleaning of the water flowing into the collecting tank 30, and avoid the waste of water when the circulating component is recycled.

In addition, it should be understood by those skilled in the art that although many problems exist in the prior art, each embodiment or technical solution of the present utility model may be modified in only one or several respects, without having to solve all technical problems listed in the prior art or the background art at the same time. Those skilled in the art will understand that nothing in one claim should be taken as a limitation on that claim.

Although terms such as a microtome body, a microtome mechanism, a transfer mechanism, a receiving end, a transfer end, a collection sink, a first sink wall, a first sink bottom, a water outlet, a notch, a flow rate regulating assembly, a slide, a water deflector, a lever, a buffer sink, a water outlet section sink wall, a receiving sink, a second sink wall, a second sink bottom, an aperture, a circulation assembly, etc., are used more herein, the possibility of using other terms is not precluded. These terms are used merely for convenience in describing and explaining the nature of the utility model; they are to be interpreted as any additional limitation that is not inconsistent with the spirit of the present utility model; the terms first, second, and the like in the description and in the claims of embodiments of the utility model and in the above-described figures, if any, are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the same; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the utility model.

Claims

1. A pathological microtome, comprising:

2. The pathological microtome according to claim 1, wherein: and a buffer water tank is arranged on the first tank wall, so that water in the collecting water tank flows into the buffer water tank and the receiving water tank in sequence through the notch.

3. The pathological microtome according to claim 2, wherein: the flow speed regulating and controlling assembly comprises a slide way arranged in the notch and two water baffles which are respectively arranged along the slide way in a reciprocating sliding way from two ends to the center.

4. A pathological microtome according to claim 3, wherein: the water baffle is provided with an operating rod which protrudes outwards towards the surface of the water baffle.

5. The pathological microtome according to claim 2, wherein: the buffer water tank comprises a water outlet section tank wall, the top surface of the water outlet section tank wall is lower than the top surface of the first tank wall, and the top surface of the water outlet section tank wall is higher than the top surface of the second tank wall.

6. The pathological microtome according to claim 1, further comprising: the circulating assembly is arranged at the bottoms of the collecting water tank and the receiving water tank;

7. The pathological microtome according to claim 6, wherein: and a plurality of openings are formed in the bottom of the second tank, so that water flowing from the collecting water tank to the receiving water tank flows into the circulating assembly through the openings.

8. The pathological microtome according to claim 6, wherein: the circulating assembly is internally provided with a filtering assembly for filtering water flowing through the circulating assembly.

9. The pathological microtome according to claim 1, wherein: the height of the receiving end is higher than the height of the transmitting end.