DE10128126A1 - Apparatus to fix histological samples has a fluid extraction near the vessel heater to pass fluid through a filter and cleaned fluid is pumped back into the closed vessel, while toxic fumes are extracted - Google Patents

Apparatus to fix histological samples has a fluid extraction near the vessel heater to pass fluid through a filter and cleaned fluid is pumped back into the closed vessel, while toxic fumes are extracted

Info

Publication number
DE10128126A1
DE10128126A1 DE2001128126 DE10128126A DE10128126A1 DE 10128126 A1 DE10128126 A1 DE 10128126A1 DE 2001128126 DE2001128126 DE 2001128126 DE 10128126 A DE10128126 A DE 10128126A DE 10128126 A1 DE10128126 A1 DE 10128126A1
Authority
DE
Germany
Prior art keywords
fluid
vessel
samples
fixing
filter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
DE2001128126
Other languages
German (de)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KÄRCHNER-WEINKAUF, HANNELORE, 96450 COBURG, DE
Original Assignee
KAERCHNER WEINKAUF HANNELORE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KAERCHNER WEINKAUF HANNELORE filed Critical KAERCHNER WEINKAUF HANNELORE
Priority to DE2001128126 priority Critical patent/DE10128126A1/en
Priority to DE20122610U priority patent/DE20122610U1/en
Publication of DE10128126A1 publication Critical patent/DE10128126A1/en
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B08—CLEANING
    • B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B15/00—Preventing escape of dirt or fumes from the area where they are produced; Collecting or removing dirt or fumes from that area
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/185—Means for temperature control using fluid heat transfer medium using a liquid as fluid

Abstract

The apparatus to clean fixer fluids during the fixing of histological samples, together with heating and the extraction of toxic vapors, has a vessel (4) to take the sample, with a closure (1) and a connection (3) connected as required to a vacuum or a pressure source. A heater (5) heats the vessel during fixing. The fixing fluid is extracted from a point near the heater, during fixing, and passed through a filter (7). A pump (6) passes the cleaned fluid into the vessel at its coldest point, to mix through the fluid in the vessel with a consistent heating action to fix the sample.

Description

Stand der TechnikState of the art

Es ist bekannt, daß bei Untersuchungen von biologischen Proben z. B. in der Histologie das gesamte zu untersuchende Gewebematerial als erstes fixiert werden muss z. B. mit Formalin oder Bouin.It is known that when examining biological samples such. B. in histology the entire tissue material to be examined must be fixed first z. B. with formalin or bouin.

Bei diesem Fixiervorgang wird das Durchdringen der Gewebe mit Fixierflüssigkeit angestrebt.This fixation process involves penetrating the tissues with fixation fluid sought.

Um das Durchdringen des Gewebes zu erreichen wird die Fixierflüssigkeit in der sich die Gewebeproben befinden gerührt oder die Gewebeproben werden in der Fixierflüssigkeit bewegt oder der Behälter mit der Fixierflüssigkeit und den Proben kann evakuiert werden mittels einer Vakuumpumpe.
Zusätzlich kann die Fixierflüssigkeit auch erwärmt werden.In order to achieve the penetration of the tissue, the fixing liquid in which the tissue samples are located is stirred or the tissue samples are moved in the fixing liquid or the container with the fixing liquid and the samples can be evacuated by means of a vacuum pump.
In addition, the fixing fluid can also be heated.

Dieser Arbeitsvorgang wird heute einschließlich des fixierens in sogenannten Entwässerungs- Automaten oder Paraffineinbettautomaten durchgeführt.This work process is today including fixing in so-called Drainage machines or paraffin embedding machines performed.

Nach dem heutigen Stand der Wissenschaft ist es zwingend erforderlich, daß histologische Untersuchungen /Diagnosen kurzfristig dem Chirurgen zur Verfügung stehen müssen.According to the current state of science, it is imperative that histological examinations / diagnoses available to the surgeon at short notice have to stand.

Die Automaten-Fixierungs-Methode ist in vielen Fällen nicht ausreichend oder sehr zeitaufwendig, speziell bei sehr fetthaltigen Gewebeproben.
Mangelhafte Fixierung erschweren die anschließende mikroskopische Untersuchung und somit die Diagnose.The automatic fixation method is in many cases insufficient or very time-consuming, especially with very fatty tissue samples.
Inadequate fixation complicates the subsequent microscopic examination and thus the diagnosis.

Bei all diesen beschriebenen Methoden wird die Fixierflüssigkeit durch ausgeschwemmte Fette und Gewebereste verunreinigt und diese verschlechtern die Qualität der Fixierflüssigkeit.With all of the methods described, the fixing fluid is passed through washed-out fats and tissue residues contaminate them and worsen them Fixing liquid quality.

Dadurch wird das Gewebe entsprechend schlecht durchtränkt und die Fixierzeiten verlängern sich erheblich.As a result, the tissue is impregnated accordingly and the fixation times lengthen considerably.

Beim öffnen der Einbettautomaten ist das ausströmen von Formalin oder sonstigen Lösungsmittel Dämpfen nicht zu verhindern und das Bedienungspersonal wird von diesen toxischen Dämpfen belastet. Um das Personal zu schützen, werden diese Automaten in besonders belüfteten Räumen installiert oder unter Abzüge gestellt.
Damit sind die Automaten ortsgebunden. When the automatic embedding machine is opened, the outflow of formalin or other solvent vapors cannot be prevented and the operating personnel are exposed to these toxic vapors. To protect the personnel, these machines are installed in specially ventilated rooms or placed under fume cupboards.
This means that the machines are local.

Beschreibung von Fig. 1Description of Fig. 1st

Vorrichtung zum fixieren von biologischen Proben.Device for fixing biological samples.

Bestehend aus:
Behälter 4, zur Aufnahme der Proben, der mit der Fixierflüssigkeit befüllbar ist. Der Behälter 4 hat einen Verschluß 1, einen Anschluß 3 an dem wechselweise Vakuum oder Druck angeschlossen werden kann.Consisting of:
Container 4 , for holding the samples, which can be filled with the fixing liquid. The container 4 has a closure 1 , a connection 3 to which alternate vacuum or pressure can be connected.

Während des Fixiervorganges kann der Behälter 4 mit der Heizung 5 beheizt werden.During the fixing process, the container 4 can be heated with the heater 5 .

Der Behälter 4 hat eine Öffnung, die mit dem Verschluß 1 mittels der Dichtung 2 verschlossen werden kann.The container 4 has an opening which can be closed with the closure 1 by means of the seal 2 .

Die Fixierflüssigkeit wird während des Fixiervorganges in der Nähe der Heizung 5 abgesaugt, durch eine Filter 7 geleitet und gereinigt somit wird erreicht, daß die erwärmte Fixierflüssigkeit durch diesen Reinigungsvorgang wieder brauchbar wird Mittels der Pumpe 6 wird die gereinigte warme Flüssigkeit an der kältesten Stelle im Behälter 4 zugeführt, dieses bewirkt eine Durchmischung der Fixierflüssigkeit und somit ist eine gleichmäßige Erwärmung für eine optimale Fixierung der Proben gewährleistet.The fixing liquid is sucked off in the vicinity of the heater 5 during the fixing process, passed through a filter 7 and cleaned, so that the heated fixing liquid is usable again by this cleaning process. By means of the pump 6 , the cleaned warm liquid is at the coldest point in the container 4 supplied, this causes a mixing of the fixing liquid and thus a uniform heating is guaranteed for an optimal fixation of the samples.

Zum Ablassen der Fixierflüssigkeit aus dem Behälter 4 ist an der tiefsten Stelle des Behälters 4 eine Abfluß mit einem Ventil 9 angeschlossen.To drain the fixing liquid from the container 4 , a drain is connected to a valve 9 at the lowest point of the container 4 .

In dem Gehäuse in dem der Gewebeprobenbehälter 4 eingebaut ist, ist an der Öffnung des Behälters 4 eine Absaugvorrichtung 11 integriert, die beim Öffnen des Verschlusses 1 automatisch die ausströmenden Dämpfe durch die Öffnung 12 über den Ventilator 8 absaugt und die Dämpfe zur Öffnung 10 weiterleitet an ein zentrales Absaugsystem oder einen Filter.In the housing in which the tissue sample container 4 is installed, a suction device 11 is integrated at the opening of the container 4 , which automatically aspirates the escaping vapors through the opening 12 via the fan 8 when the closure 1 is opened and forwards the vapors to the opening 10 a central suction system or a filter.

Claims (1)

Verfahren zur Reinigung der Fixierflüssigkeit während der Fixierung histologischer Proben bei gleichmäßiger Erwärmung sowie zum absaugen toxischer Dämpfe,
dadurch gekennzeichnet,
daß bei dem hier erläuterten Verfahren, eine Pumpe die Fixierflüssigkeit aus dem Probenbehälter saugt oder drückt und sie diesem an anderer Stelle wieder zuführt. Die Flüssigkeitsentnahme findet am oberen oder unteren Bereich des Probenbehälters statt. Es entsteht ein geschlossener Kreislauf in welchem ein Filter zur Reinigung und Fettabscheidung eingebaut ist, dadurch kann die Fixierflüssigkeit länger verwendet werden und die Umwelt wird weniger belastet.
Ein weiterer Vorteil dieses Kreislauf ist es, daß durch die Zirkulation der Fixierflüssigkeit eine gleichmäßige Wärmeverteilung statt findet und dadurch die Gewebeproben schneller und gleichmäßiger fixiert werden.
Zusätzlich wird wechselweise Über- oder Unterdruck angelegt, um die in den Proben eingeschlossenen Luftblasen zu entfernen. Auch dieses trägt zur schnelleren und gleichmäßigeren Fixierung der Proben bei.
Zum Schutze des Bedienungspersonals sowie zur Entlastung der Umwelt ist eine Absaugvorrichtung am oberen Verschlußrand des Probenbehälters eingebaut die sich automatisch beim öffnen des Verschlusses einschaltet. Die abgesaugten Dämpfe werden nun durch einen angeschlossenen Filter gereinigt, dadurch ist das Gerät nicht ortsgebunden und kann überall im Labor aufgestellt werden.Process for cleaning the fixation liquid during the fixation of histological samples with uniform heating and for sucking off toxic vapors,
characterized by
that in the method explained here, a pump sucks or presses the fixing liquid from the sample container and feeds it again at another point. The liquid is drawn from the upper or lower area of the sample container. This creates a closed circuit in which a filter for cleaning and grease separation is built in, which means that the fixing fluid can be used longer and the environment is less polluted.
Another advantage of this circuit is that the circulation of the fixing liquid results in an even heat distribution and thus the tissue samples are fixed faster and more uniformly.
In addition, positive or negative pressure is alternately applied to remove the air bubbles trapped in the samples. This also helps to fix the samples faster and more evenly.
To protect the operating personnel and to relieve the environment, a suction device is installed on the upper closure edge of the sample container, which switches on automatically when the closure is opened. The extracted vapors are now cleaned by a connected filter, which means that the device is not tied to a specific location and can be set up anywhere in the laboratory.
DE2001128126 2001-06-09 2001-06-09 Apparatus to fix histological samples has a fluid extraction near the vessel heater to pass fluid through a filter and cleaned fluid is pumped back into the closed vessel, while toxic fumes are extracted Ceased DE10128126A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE2001128126 DE10128126A1 (en) 2001-06-09 2001-06-09 Apparatus to fix histological samples has a fluid extraction near the vessel heater to pass fluid through a filter and cleaned fluid is pumped back into the closed vessel, while toxic fumes are extracted
DE20122610U DE20122610U1 (en) 2001-06-09 2001-06-09 Apparatus to fix histological samples has a fluid extraction near the vessel heater to pass fluid through a filter and cleaned fluid is pumped back into the closed vessel, while toxic fumes are extracted

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2001128126 DE10128126A1 (en) 2001-06-09 2001-06-09 Apparatus to fix histological samples has a fluid extraction near the vessel heater to pass fluid through a filter and cleaned fluid is pumped back into the closed vessel, while toxic fumes are extracted

Publications (1)

Publication Number Publication Date
DE10128126A1 true DE10128126A1 (en) 2002-12-12

Family

ID=7687812

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DE2001128126 Ceased DE10128126A1 (en) 2001-06-09 2001-06-09 Apparatus to fix histological samples has a fluid extraction near the vessel heater to pass fluid through a filter and cleaned fluid is pumped back into the closed vessel, while toxic fumes are extracted

Country Status (1)

Country Link
DE (1) DE10128126A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1279444A2 (en) * 2001-07-23 2003-01-29 Thermo Shandon Limited Processor device with fume extraction
DE102008039876A1 (en) 2008-08-27 2010-03-11 Leica Biosystems Nussloch Gmbh A method of controlling a tissue processor and tissue processor
WO2012166776A1 (en) * 2011-06-03 2012-12-06 Preyas Shah Rotary tissue processor with configurable stations

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1279444A2 (en) * 2001-07-23 2003-01-29 Thermo Shandon Limited Processor device with fume extraction
EP1279444A3 (en) * 2001-07-23 2004-02-04 Thermo Shandon Limited Processor device with fume extraction
DE102008039876A1 (en) 2008-08-27 2010-03-11 Leica Biosystems Nussloch Gmbh A method of controlling a tissue processor and tissue processor
US8039262B2 (en) 2008-08-27 2011-10-18 Leica Biosystems Nussloch Gmbh Method for controlling a tissue processor and tissue processor
WO2012166776A1 (en) * 2011-06-03 2012-12-06 Preyas Shah Rotary tissue processor with configurable stations
US8920720B2 (en) 2011-06-03 2014-12-30 Rushabh Instruments, Inc. Rotary tissue processor with configurable stations

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Legal Events

Date Code Title Description
8127 New person/name/address of the applicant

Owner name: KÄRCHNER-WEINKAUF, HANNELORE, 96450 COBURG, DE

8110 Request for examination paragraph 44
8127 New person/name/address of the applicant

Owner name: KäRCHNER-WEINKAUF, HANNELORE, 91301 FORCHHEIM, DE

8131 Rejection