CN112284848B - Full-automatic paraffin section making devices - Google Patents

Full-automatic paraffin section making devices Download PDF

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Publication number
CN112284848B
CN112284848B CN202011127933.9A CN202011127933A CN112284848B CN 112284848 B CN112284848 B CN 112284848B CN 202011127933 A CN202011127933 A CN 202011127933A CN 112284848 B CN112284848 B CN 112284848B
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automatic
component
dehydration
plate
liquid
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CN112284848A (en
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温静静
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36Embedding or analogous mounting of samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873Cutting or cleaving
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36Embedding or analogous mounting of samples
    • G01N2001/366Moulds; Demoulding

Abstract

The invention relates to the technical field of clinical pathological sections, and discloses a full-automatic paraffin section manufacturing device which comprises an automatic dehydration component capable of dehydrating and coating paraffin, an automatic section component capable of completing paraffin section, a storage component arranged in the automatic dehydration component and used for storing tissue materials, a control mechanical arm for placing the storage component on the automatic section component from the automatic dehydration component, and a control component electrically connected with the automatic dehydration component, the automatic section component, the storage component and the control mechanical arm. The device can realize the dehydration, the wax coating and the slicing of tissue materials, the automatic and integrated operation of the whole process can reduce the workload of medical personnel and effectively improve the manufacturing efficiency of paraffin slices.

Description

Full-automatic paraffin section making devices
Technical Field
The invention relates to the technical field of clinical pathological sections, in particular to a full-automatic paraffin section manufacturing device.
Background
The pathology department is one of indispensable departments of large-scale comprehensive hospitals, and the main task of the pathology department is to undertake pathological diagnosis work in the medical process, wherein the pathological diagnosis work comprises biopsy, falling and fine needle puncture cytology examination and autopsy examination, so that clear pathological diagnosis is provided for clinic, the nature of diseases is determined, and the death cause is found out; at present, dehydration and slicing of pathological tissues in hospitals are routine steps in pathological examination; the pathological section is one kind of pathological specimen, and is prepared through treating partial pathological tissue or organ with various chemicals and embedding method to make it fixed and hardened, slicing in slicer, adhering to glass sheet, dyeing in different colors, and examination under microscope to observe pathological change and make pathological diagnosis.
There are solitary automatic dehydration, automatic section device among the current device in existing market, automatic dehydration device need be manual to wax stone processing after accomplishing wax packing, is cut into the square, has increased medical personnel's work load, and has restricted pathological section's preparation efficiency greatly.
Disclosure of Invention
The technical problem solved by the invention is as follows: the device can realize the dehydration, the wax coating and the slicing of tissue materials, the automatic and integrated operation of the whole process can reduce the workload of medical personnel and effectively improve the manufacturing efficiency of paraffin slices.
The technical scheme of the invention is as follows: a full-automatic paraffin section manufacturing device comprises an automatic dehydration assembly capable of completing dehydration and wax wrapping, an automatic section assembly capable of completing wax block section, a storage assembly movably arranged in the automatic dehydration assembly and used for storing tissue materials, a control mechanical arm used for placing the storage assembly on the automatic section assembly from the automatic dehydration assembly, and a control assembly electrically connected with the automatic dehydration assembly, the automatic section assembly, the storage assembly and the control mechanical arm;
the automatic slicing assembly comprises a slicing assembly and a fixing assembly arranged on one side of the slicing assembly; the fixing assembly comprises a support frame, a tray bracket movably arranged on the support frame, a linear motor arranged on the support frame and used for controlling the tray bracket, a rotating motor arranged on the tray bracket, and a clamping lock arranged on the rotating motor;
the storage assembly comprises a circular tray, a plurality of tissue storage boxes which are arranged on the circular tray and can be used for independently storing pathological tissue materials, a positioning triangular cone arranged on the upper surface of the circular tray, and a fixing groove which is arranged on the lower surface of the circular tray and can be connected with a clamping lock;
the control mechanical arm comprises a mechanical arm mounting frame, six robots arranged on the mechanical arm mounting frame, and grabbing components which are arranged on the six robots and can grab the circular tray;
the tissue storage boxes comprise square wax block fixing plates arranged on the circular tray, connecting rods clamped at four corners of the wax block fixing plates, stripper plates made of iron-containing materials and fixedly arranged at the upper ends of the connecting rods, and liquid exchange plates which are fixedly surrounded on the periphery of the connecting rods and are provided with circular small holes;
the grabbing component comprises a grabbing plate, a positioner arranged on the grabbing plate and capable of being occluded with a positioning triangular cone, a fixing claw movably arranged on the grabbing plate and capable of clamping a circular tray, a scraper capable of cutting redundant paraffin around the liquid changing plate and an electromagnetic chuck arranged on the grabbing plate and capable of attracting the stripper plate.
Furthermore, aluminum foils for reducing friction force are arranged on the surfaces of the connecting rod, the demoulding plate and the liquid changing plate, and bulges for increasing paraffin attachment surfaces are arranged on the surface of the wax block fixing plate; the setting of aluminium foil can reduce the frictional force of paraffin piece and connecting rod, stripper plate, trade the liquid board when the drawing of patterns, and bellied setting can strengthen the adhesive force of paraffin piece on the wax piece fixed plate, and this is favorable to guaranteeing the smooth drawing of patterns of wax piece, avoids damaging inside tissue material.
Furthermore, the fixed claw comprises 3 telescopic rods uniformly distributed at the center of the grabbing disc, and claw hooks which are slidably arranged on the telescopic rods and can grab the circular tray; the distance between the tissue storage box and the scraper can be shortened after the circular tray is grabbed through the telescopic rod, so that the clean cutting of redundant paraffin on the side surface of the tissue storage box is realized, and the slicing quality is favorably improved.
Furthermore, the slicing assembly comprises a knife rest, a blade and a motor assembly, wherein the knife rest is movably arranged on the support frame through a high-precision rolling shaft lead screw and is positioned on one side of the fixing assembly; through the removal of the control knife rest of high accuracy roller lead screw, can realize the accurate control to the blade, ensure sliced validity, accurate control section thickness.
Furthermore, the automatic dehydration component adopts an automatic pathological dehydrator, and a replenishing liquid component for replenishing fixed liquid, dehydrated liquid and transparent liquid to the dehydration cylinder group is arranged on the automatic pathological dehydrator; the liquid supplementing assembly comprises a liquid storage cylinder group, a negative pressure pump group and a circulating pipe, wherein the negative pressure pump group is used for communicating the liquid storage cylinder group with the dehydration cylinder group, and the circulating pipe is used for communicating the upper end of the side surface of the dehydration cylinder group with the liquid storage cylinder group; the liquid supplementing assembly can compensate the medicament solution in the automatic pathological dehydrator and enable the medicament solution to flow in the first cylinder group, so that the speed of fixing and dehydrating cells in the tissue material can be increased, and the treatment efficiency of the tissue material is improved.
Furthermore, the dehydration cylinder groups of the automatic pathological dehydrator are movably provided with sealing covers, and linear motors capable of controlling the sealing covers to open and close are fixed on the dehydration cylinder groups; the sealing cover is provided with a sealing strip for preventing harmful gas from escaping; the sealing cover is provided with a sealing strip for preventing harmful gas from escaping; the setting of sealed lid, sealing strip can tentatively alleviate harmful gas's diffusion, reduces the harm to medical personnel, and linear electric motor's setting can make sealed lid realize electric control, just operates through the touch screen with medical personnel.
Furthermore, a protective box body is arranged outside the automatic dehydration component, and a waste gas treatment component is arranged on the protective box body; the waste gas treatment component comprises a sterilization component capable of ionizing harmful gas and an activated carbon filtering component; the arrangement of the protective box body can isolate harmful gas which cannot be blocked by the sealing cover and the sealing strip, and prevent the harmful gas from diffusing to the outside; the sterilizing component for ionized harmful gas and the active carbon filtering component can treat the harmful gas in the protective box body and finally discharge harmless gas.
The invention has the beneficial effects that: the device can realize dehydration, wax coating and slicing of tissue materials, and the whole process is automated and integrated, so that the workload of medical personnel is reduced, and the manufacturing efficiency of paraffin slicing is effectively improved; according to the invention, the six-axis robot is provided with the grabbing component, and the storage component is connected with the automatic pathological dehydrator and the automatic pathological microtome; the grabbing component is provided with the scraper, so that redundant paraffin on the tissue storage box can be cut off, and the paraffin block can be separated from the stripper plate and the liquid changing plate through the electromagnetic chuck, so that the paraffin block forms a neat square shape, and the automatic slicing component is favorable for processing; through set up a plurality of tissue material case on circular tray, can realize once handling the section of a plurality of pathological tissue materials, improve the sliced efficiency of preparation greatly.
Drawings
FIG. 1 is a schematic structural view of embodiment 1 of the present invention;
FIG. 2 is a schematic view showing the structure of the depositing unit and the grasping unit in the embodiment 1 of the present invention;
FIG. 3 is a schematic structural view of a tissue storage cassette according to embodiment 1 of the present invention;
FIG. 4 is a schematic view of the automatic slicing assembly according to embodiment 1 of the present invention;
wherein, 1-an automatic dehydration component, 11-a liquid storage cylinder group, 12-a negative pressure pump group, 13-a circulating pipe, 2-an automatic slicing component, 21-a slicing component, 211-a knife rest, 22-a fixing component, 221-a tray bracket, 222-a clamping lock, 3-a storage component, 31-a circular tray, 32-an organization storage box, 321-a wax block fixing plate, 322-a connecting rod, 323-demoulding plate, 324-liquid changing plate, 33-positioning triangular cone, 34-fixing groove, 4-control mechanical arm, 41-mechanical arm mounting rack, 42-six-axis robot, 43-grabbing component, 431-grabbing plate, 432-positioner, 433-fixing claw, 434-scraper, 435-electromagnetic sucker and 5-control component.
Detailed Description
Example 1: as shown in fig. 1, 2, 3, 4, a fully automatic paraffin section making apparatus comprises an automatic dehydration module 1 capable of dehydrating and coating paraffin, an automatic section module 2 capable of completing wax block section, a storage module 3 movably disposed in the automatic dehydration module 1 and storing tissue material, a control mechanical arm 4 for placing the storage module 3 from the automatic dehydration module 1 to the automatic section module 2, and a control module 5 electrically connected to the automatic dehydration module 1, the automatic section module 2, the storage module 3, and the control mechanical arm 4;
the automatic slicing assembly 2 comprises a slicing assembly 21, a fixing assembly 22 arranged on one side of the slicing assembly 21; the fixing assembly 22 comprises a support frame, a tray bracket 221 movably arranged on the support frame, a linear motor arranged on the support frame and used for controlling the tray bracket 221, a rotating motor arranged on the tray bracket 221, and a clamping lock 222 arranged on the rotating motor;
the storage assembly 3 comprises a circular tray 31, a plurality of tissue storage boxes 32 which are arranged on the circular tray 31 and can be used for independently storing pathological tissue materials, a positioning triangular cone 33 arranged on the upper surface of the circular tray 31, and a fixing groove 34 which is arranged on the lower surface of the circular tray 31 and can be connected with a clamping lock 222;
the control mechanical arm 4 comprises a mechanical arm mounting frame 41, a six-axis robot 42 arranged on the mechanical arm mounting frame 41, and a grabbing component 43 which is arranged on the six-axis robot 42 and can grab the circular tray 31;
the tissue storage boxes 32 respectively comprise a square wax block fixing plate 321 arranged on the circular tray 31, connecting rods 322 clamped at four corners of the wax block fixing plate 321, a stripping plate 323 fixed at the upper end of the connecting rods 322 and made of iron-containing materials, and liquid exchange plates 324 fixedly surrounded on the periphery of the connecting rods 322 and provided with circular small holes;
the grabbing component 43 comprises a grabbing plate 431, a positioner 432 which is arranged on the grabbing plate 431 and can be meshed with the positioning triangular cone 33, a fixed claw 433 which is movably arranged on the grabbing plate 431 and can clamp the circular tray 31, a scraper 434 which can cut off redundant paraffin around the liquid changing plate 324, and an electromagnetic suction cup 435 which is arranged on the grabbing plate 431 and can suck the demoulding plate 323.
As shown in fig. 1, the automatic dehydration assembly 1 adopts an automatic pathological dehydrator, and a replenishment liquid assembly for replenishing fixed liquid, dehydration liquid and transparent liquid to a dehydration cylinder group is arranged on the automatic pathological dehydrator; the liquid supplementing assembly comprises a liquid storage cylinder group 11, a negative pressure pump group 12 for communicating the liquid storage cylinder group 11 with the dewatering cylinder group, and a circulating pipe 13 for communicating the upper end of the side surface of the dewatering cylinder group with the liquid storage cylinder group 11.
The surfaces of the connecting rod 322, the demoulding plate 323 and the liquid exchange plate 324 are all provided with aluminum foils for reducing friction force, and the surface of the wax block fixing plate 321 is provided with a bulge for increasing a paraffin attachment surface.
As shown in fig. 2, the fixed claw 433 includes 3 telescopic rods uniformly arranged around the center of the grabbing plate 431, and a claw hook slidably mounted on the telescopic rods and capable of grabbing the circular tray 31.
As shown in fig. 4, the slicing assembly 21 includes a knife rest 211 movably disposed on the supporting frame through a high-precision roller screw and located on one side of the fixing assembly 22, a blade disposed on the knife rest 211, and a motor assembly disposed on the knife rest 211 for controlling the blade to slice the wax block.
Wherein, the control component 5 adopts a PLC controller; the automatic pathological dehydrator, the rotating motor, the linear motor, the high-precision roller lead screw, the motor assembly, the positioner 432 and the electromagnetic chuck 435 are all commercially available products.
The working steps are as follows: firstly, medical staff respectively put pathological tissue materials into the tissue storage boxes 32 and clamp the connecting rods 322 with the wax block fixing plates 321; the fixed claws 433 on the grabbing component 43 grab the circular tray 31, and the circular tray 31 is sequentially placed into the automatic dehydration component under the control of the six-axis robot 42 to be sequentially fixed, dehydrated, transparent, wax-coated and the like; after the paraffin coating is finished, the six-axis robot controls the grabbing component 43 to take out the circular tray 31, and then the scraper 434 cuts off redundant paraffin on the tissue storage box; finally, the grabbing component 43 connects the fixing groove 34 on the circular tray 31 with the clamping lock 222 on the fixing component 22, the fixing claw 433 releases the circular tray 31, the electromagnetic suction disc 435 sucks and separates the stripping plate 323 from the paraffin block, the tissue material wrapping the paraffin is exposed, and the slicing component 21 performs slicing.
Example 2: different from the embodiment 1, the dewatering cylinder groups of the automatic pathological dewatering machine are movably provided with sealing covers, and linear motors capable of controlling the sealing covers to open and close are fixed on the sealing cylinder groups; the sealing cover is provided with a sealing strip for preventing harmful gas from escaping.
A protective box body is arranged outside the automatic dehydration component 1, and a waste gas treatment component is arranged on the protective box body; the waste gas treatment component comprises a sterilizing component capable of ionizing harmful gas and an activated carbon filtering component.
Wherein, the sterilization component adopts a commercially available high-voltage electrostatic air purification device, and the active carbon filtering component adopts a commercially available active carbon filter screen.

Claims (6)

1. A full-automatic paraffin section manufacturing device for clinical pathology department is characterized by comprising an automatic dehydration component (1) capable of dehydrating and coating paraffin, an automatic section component (2) capable of completing wax block slicing, a storage component (3) movably arranged in the automatic dehydration component (1) and used for storing tissue materials, a control mechanical arm (4) for placing the storage component (3) in the automatic section component (2) from the automatic dehydration component (1), and a control component (5) electrically connected with the automatic dehydration component (1), the automatic section component (2), the storage component (3) and the control mechanical arm (4);
the automatic slicing assembly (2) comprises a slicing assembly (21) and a fixing assembly (22) arranged on one side of the slicing assembly (21); the fixing assembly (22) comprises a support frame, a tray support (221) movably arranged on the support frame, a linear motor arranged on the support frame and used for controlling the tray support (221), a rotating motor arranged on the tray support (221), and a clamping lock (222) arranged on the rotating motor;
the storage assembly (3) comprises a circular tray (31), a plurality of tissue storage boxes (32) which are arranged on the circular tray (31) and can be used for independently storing pathological tissue materials, a positioning triangular cone (33) arranged on the upper surface of the circular tray (31), and a fixing groove (34) which is arranged on the lower surface of the circular tray (31) and can be connected with a clamping lock (222);
the control mechanical arm (4) comprises a mechanical arm mounting frame (41), a six-axis robot (42) arranged on the mechanical arm mounting frame (41), and a grabbing component (43) which is arranged on the six-axis robot (42) and can grab the circular tray (31);
the tissue storage boxes (32) respectively comprise a square wax block fixing plate (321) arranged on the circular tray (31), connecting rods (322) clamped at four corners of the wax block fixing plate (321), a stripper plate (323) fixed at the upper end of the connecting rods (322) and made of iron-containing materials, and liquid changing plates (324) fixedly surrounded on the periphery of the connecting rods (322) and provided with circular small holes;
the grabbing component (43) comprises a grabbing plate (431), a positioner (432) which is arranged on the grabbing plate (431) and can be occluded with the positioning triangular cone (33), a fixed claw (433) which is movably arranged on the grabbing plate (431) and can clamp the circular tray (31), a scraper (434) which can cut off the redundant paraffin on the periphery of the liquid changing plate (324), and an electromagnetic suction cup (435) which is arranged on the grabbing plate (431) and can suck the demoulding plate (323);
the surface of the connecting rod (322), the surface of the stripping plate (323) and the surface of the liquid exchange plate (324) are all provided with aluminum foils for reducing friction force, and the surface of the wax block fixing plate (321) is provided with bulges for increasing paraffin attachment surfaces.
2. The fully-automatic paraffin section making device for clinical pathology department according to claim 1, wherein the fixed claw (433) comprises 3 telescopic rods uniformly arranged on the outer side wall of the grabbing plate (431), and claw hooks slidably mounted on the telescopic rods and capable of grabbing the circular tray (31).
3. The fully automatic paraffin section making device for clinical pathology department according to claim 1, characterized in that, the section subassembly (21) includes a knife rest (211) movably arranged on the supporting frame through a high precision roller lead screw and located on one side of the fixed subassembly (22), a blade arranged on the knife rest (211), and a motor subassembly arranged on the knife rest (211) for controlling the blade to slice the wax block.
4. The full-automatic paraffin section manufacturing device for clinical pathology department according to claim 1, characterized in that the automatic dehydration component (1) adopts an automatic pathology dehydrator, and the automatic pathology dehydrator is provided with a liquid supplementing component for supplementing a dehydration cylinder group with stationary liquid, dehydration liquid and transparent liquid; the liquid supplementing assembly comprises a liquid storage cylinder group (11), a negative pressure pump group (12) communicated with the liquid storage cylinder group (11) and the dewatering cylinder group, and a circulating pipe (13) communicated with the liquid storage cylinder group (11) and arranged at the upper end of the side surface of the dewatering cylinder group.
5. The fully automatic paraffin section making device for clinical pathology department according to claim 4, wherein the dehydration cylinders of the automatic pathology dehydrator are movably provided with sealing covers, and linear motors capable of controlling the sealing covers to open and close are fixed on the dehydration cylinders; and the sealing cover is provided with a sealing strip for preventing harmful gas from escaping.
6. The full-automatic paraffin section manufacturing device for clinical pathology department according to claim 5, characterized in that a protection box body is arranged outside the automatic dehydration component (1), and an exhaust gas treatment component is arranged on the protection box body; the waste gas treatment component comprises a sterilization component capable of ionizing harmful gas and an activated carbon filtering component.
CN202011127933.9A 2020-10-20 2020-10-20 Full-automatic paraffin section making devices Active CN112284848B (en)

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Application Number Priority Date Filing Date Title
CN202011127933.9A CN112284848B (en) 2020-10-20 2020-10-20 Full-automatic paraffin section making devices

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Application Number Priority Date Filing Date Title
CN202011127933.9A CN112284848B (en) 2020-10-20 2020-10-20 Full-automatic paraffin section making devices

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CN112284848B true CN112284848B (en) 2022-07-22

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