WO2012050011A1 - 単一発光粒子検出を用いた粒子の拡散特性値の測定方法 - Google Patents
単一発光粒子検出を用いた粒子の拡散特性値の測定方法 Download PDFInfo
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Definitions
- the present invention uses an optical system capable of detecting light from a minute region in a solution, such as an optical system of a confocal microscope or a multiphoton microscope, and uses atoms, molecules or aggregates thereof dispersed or dissolved in a solution ( These are hereinafter referred to as “particles”), for example, biomolecules such as proteins, peptides, nucleic acids, lipids, sugar chains, amino acids or aggregates thereof, particulate objects such as viruses and cells, or non-
- the present invention relates to an optical analysis method capable of detecting light from biological particles and obtaining useful information in analysis or analysis of their states (interactions, binding / dissociation states, etc.).
- the present invention relates to a method that enables various optical analyzes by individually detecting light from a single light emitting particle using the optical system as described above.
- a particle that emits light (hereinafter referred to as “luminescent particle”) is either a particle that emits light itself, or a particle to which an arbitrary luminescent label or luminescent probe is added.
- the light emitted from the luminescent particles may be fluorescence, phosphorescence, chemiluminescence, bioluminescence, scattered light, or the like.
- the average residence time (translational diffusion time) of the fluorescent molecules and the like in the minute region determined from the value of the autocorrelation function of the measured fluorescence intensity, and the average number of staying molecules Based on this, acquisition of information such as the speed or size of movement of fluorescent molecules, concentration, concentration, molecular structure or size change, molecular binding / dissociation reaction, dispersion / aggregation, etc.
- fluorescence intensity distribution analysis Fluorescence-Intensity Distribution Analysis: FIDA.
- Patent Document 4 Non-Patent Document 4 and Photon Counting Histogram (PCH.
- Patent Document 5 are measured in the same manner as FCS.
- a histogram of the fluorescence intensity of the fluorescent molecules entering and exiting the confocal volume generated is generated, and by fitting a statistical model formula to the distribution of the histogram, the average of the intrinsic brightness of the fluorescent molecules etc.
- the average value of the value and the number of molecules staying in the confocal volume is calculated, and based on this information, changes in the structure or size of the molecule, binding / dissociation state, dispersion / aggregation state, etc. are estimated. It will be.
- Patent Documents 6 and 7 propose a method for detecting a fluorescent substance based on the passage of time of a fluorescence signal of a sample solution measured using an optical system of a confocal microscope.
- Patent Document 8 describes the presence of fluorescent fine particles in a flow or on a substrate by measuring weak light from fluorescent fine particles distributed in a flow cytometer or fluorescent fine particles fixed on a substrate using a photon counting technique. A signal arithmetic processing technique for detecting the signal is proposed.
- the sample required for measurement has an extremely low concentration compared with the conventional method. It can be very small (the amount used in one measurement is about several tens of ⁇ L), and the measurement time is greatly shortened (measurement of time on the order of seconds is repeated several times in one measurement). . Therefore, these technologies are particularly useful for analyzing rare or expensive samples often used in the field of medical and biological research and development, for clinical diagnosis of diseases, screening for physiologically active substances, etc. When the number is large, it is expected to be a powerful tool capable of performing experiments or inspections at a lower cost or faster than conventional biochemical methods.
- the light to be measured is light emitted from one or several fluorescent molecules.
- statistical processing such as calculation of fluctuation of fluorescence intensity such as calculation of autocorrelation function of fluorescence intensity data measured in time series or fitting to histogram is executed, and light signals from individual fluorescent molecules etc. are processed. It is not individually referenced or analyzed. That is, in these photoanalysis techniques, light signals from a plurality of fluorescent molecules and the like are statistically processed, and statistical average characteristics of the fluorescent molecules and the like are detected.
- the concentration or number density of the fluorescent molecules or the like to be observed in the sample solution is equal to one second in the equilibrium state.
- the number of fluorescent molecules that can be statistically processed within the measurement time of the order length enters and exits the micro area, and preferably there is always about one fluorescent molecule in the micro area.
- the concentration of fluorescent molecules or the like in the sample solution used in the above optical analysis technique is typically about 1 nM or more. When it is significantly lower than 1 nM, a time when the fluorescent molecule or the like is not present in the confocal volume occurs, and a statistically significant analysis result cannot be obtained.
- the methods for detecting fluorescent molecules described in Patent Documents 6 to 8 do not include statistical calculation processing of fluctuations in fluorescence intensity, and even if the fluorescent molecules in the sample solution are less than 1 nM, the fluorescent molecules However, it has not been achieved to quantitatively calculate the concentration or number density of fluorescent molecules that are moving randomly in the solution.
- the applicant of the present application deals with the optical analysis technique including the statistical processing such as FCS, FIDA, etc. in the concentration or number density of the luminescent particles to be observed in Japanese Patent Application No. 2010-044714 and PCT / JP2011 / 53481.
- An optical analysis technique based on a novel principle that makes it possible to quantitatively observe the state or characteristics of luminescent particles in a sample solution below a certain level is proposed.
- an optical system capable of detecting light from a minute region in a solution such as an optical system of a confocal microscope or a multiphoton microscope, like FCS, FIDA and the like.
- the photodetection region When used, while moving the position of a minute region (hereinafter referred to as “light detection region”) that is a light detection region in the sample solution, that is, while scanning the sample solution by the light detection region,
- the photodetection region includes luminescent particles that are dispersed in the sample solution and move randomly, the light emitted from the luminescent particles is detected, and each luminescent particle in the sample solution is individually detected
- the sample required for measurement is a very small amount (for example, about several tens of ⁇ L) as in the optical analysis techniques such as FCS and FIDA.
- the measurement time is short, and the presence of luminescent particles having a lower concentration or number density is detected as compared with the case of optical analysis techniques such as FCS and FIDA, and the concentration, number density or Other characteristics can be detected quantitatively.
- the photodetection region is typically moved so as to circulate through a predetermined path such as, for example, a circle or an ellipse.
- a predetermined path such as, for example, a circle or an ellipse.
- the main object of the present invention is to use the detection method of the luminescent particles of the scanning molecule counting method to indicate an index value indicating the ease of movement of the luminescent particles due to Brownian motion, typically the diffusion coefficient of the luminescent particles. It is to provide a new method of measuring
- Another object of the present invention is to facilitate movement of luminescent particles by Brownian motion in a sample solution having a concentration lower than the concentration of luminescent particles that can be measured with good accuracy by optical analysis technology such as FCS. It is to provide a method for measuring the index value representing the thickness or the diffusion coefficient of the luminescent particles.
- the above-described problem is a method for measuring the diffusion characteristic value of luminescent particles dispersed and moving randomly in a sample solution using an optical system of a confocal microscope or a multiphoton microscope,
- the process of periodically moving the position of the light detection area of the optical system along the predetermined path in the sample solution by changing the optical path of the optical system, and the position of the light detection area in the sample solution The process of generating the light intensity data by measuring the light intensity from the light detection area while moving the light, the process of individually detecting the signal representing the light of the luminescent particles in the light intensity data, and the detected Based on the process of extracting a plurality of signals corresponding to the same luminescent particle among the signals representing the light of the luminescent particles, and the deviation time from the moving period of the light detection region in the interval of the generation time of the extracted signals
- the signal corresponding to the extracted signal It is achieved by a method which comprises a step of calculating the diffusion characteristic values of the particle.
- a light-emitting particle dispersed in a sample solution and moving randomly is a particle that emits light, such as atoms, molecules, or aggregates thereof dispersed or dissolved in a sample solution. Any particle may be used as long as it is not fixed to the substrate or the like and freely moves in the solution in Brownian motion.
- luminescent particles are typically fluorescent particles, but may be particles that emit light by phosphorescence, chemiluminescence, bioluminescence, light scattering, or the like.
- the “light detection area” of the optical system of a confocal microscope or multiphoton microscope is a minute area in which light is detected in those microscopes.
- the illumination light is Corresponds to the focused region (in the confocal microscope, it is determined in particular by the positional relationship between the objective lens and the pinhole.
- the luminescent particles emit light without illumination light, for example, chemiluminescence or biological In the case of particles that emit light by light emission, illumination light is not required in the microscope.
- the “diffusion characteristic value” may be an arbitrary index value representing the ease of movement of particles due to Brownian motion, and is typically a diffusion coefficient of particles, but other physical quantities, for example, It may be a function of translational diffusion time, arbitrary diffusion coefficient.
- the term “signal” refers to a signal representing light from the luminescent particles unless otherwise specified.
- the position of the photodetection region is moved in the sample solution, that is, the sample solution is scanned by the photodetection region. Sequentially, light detection is performed. Then, when the moving light detection region includes randomly moving luminescent particles, light from the luminescent particles is detected, and thereby the presence of one luminescent particle is detected.
- the light detection region when the light detection region periodically moves along a predetermined path, the light detection region wraps around the predetermined path after the light detection region includes a certain light emitting particle at a certain position, and the light emitting particle If the luminescent particle does not deviate from the predetermined path of the light detection region while reaching the vicinity of the position including the light, the luminescent particle is again included in the light detection region and the light is detected. . However, since the position of the luminescent particle is moved by Brownian motion while the light detection region makes a round of the predetermined path, the interval between the time when the luminescent particle is first detected and the time when the luminescent particle is detected next is detected.
- the interval of the generation time of the signal of the luminescent particles does not completely coincide with the movement period of the light detection region, and deviates from the movement period of the light detection region. It is expected that the ease of movement of the luminous particles due to the Brownian motion is reflected. Therefore, in the present invention, as described above, a plurality of signals corresponding to the same luminescent particles are extracted from the signals representing the light of the luminescent particles detected on the light intensity data, and these extracted signals are extracted. The diffusion characteristic value of the luminescent particles corresponding to the extracted signal is calculated based on the shift time from the movement period of the light detection region in the generation time interval.
- the diffusion characteristic value can be individually measured. Therefore, for calculating the fluctuation of the fluorescence intensity in the optical analysis technology such as FCS.
- Statistical processing is not required, and it is advantageous in that the diffusion characteristic value of the luminescent particles can be obtained even when the concentration of the luminescent particles in the sample solution is lower than the level necessary for obtaining a good measurement result by FCS or the like.
- the diffusion characteristic value is calculated based on the signal generation time on the light intensity data (one-dimensional data), the calculation load is not so large. It is advantageous.
- the displacement of the luminescent particles detected by the above-described method is the difference between the deviation time from the period of the light detection region and the light in the generation time interval of the signal extracted as the same luminescent particle signal on the light intensity data. It is calculated from the moving speed of the detection area. Therefore, in the above calculation of the diffusion characteristic value, the displacement of the luminescent particles may be calculated first, and the diffusion characteristic value of the luminescent particles may be calculated based on the displacement of the luminescent particles.
- the interval between the generation times of the signals extracted as signals of the same luminescent particles on the light intensity data is not necessarily a difference in the generation times of adjacent signals in time, but a plurality of consecutive extracted signals. It should be understood that it may be the time difference between the occurrence times of any two of the signals.
- the time interval between the generation of the two signals is the diffusion characteristic value or diffusion. It may be used to calculate the coefficient. That is, when n signals are extracted, n (n ⁇ 1) / 2 signal generation time interval values are obtained, and the generation characteristic interval value or the generation time interval value is obtained using these generation time interval values.
- a diffusion coefficient may be calculated. This method is advantageous in that many displacement values can be obtained in a short measurement time, and a highly reliable calculation result can be obtained, compared with the case of sequentially measuring the displacement of particles from the difference in generation time of adjacent signals. It is.
- the time at which one signal out of the signals representing the light of the detected luminescent particles is generated.
- the signal of the same luminescent particle as the luminescent particle corresponding to the one signal is generated within the time width determined based on the size and moving speed of the light detection region from the time obtained by adding the period of the light detection region. It may be determined that As described above, in the method of the present invention, the same light emitting particles are periodically detected every time the light detection region goes around a predetermined path (rather than continuously tracking one light emitting particle). Then, the diffusion characteristic value or diffusion coefficient is calculated from the interval or displacement of the signal generation time.
- the diffusion characteristic value or the diffusion coefficient is so large that the (average) displacement of the luminescent particles exceeds the size of the light detection region in the time (period) in which the light detection region circulates, the same light emission
- the particle signal may not be detected periodically. That is, conversely, when the displacement of the luminescent particles in the time that the photodetection region circulates is smaller than the size of the photodetection region, the signal interval of the luminescent particles is the period of the photodetection region,
- the size of the photodetection area falls within a range of values obtained by adding or subtracting half the time width required for the photodetection area to move.
- the time width required for the light detection region to move in the size of the light detection region is determined based on the size and movement speed of the light detection region, a certain signal and the signal are generated. Thereafter, the signal generated within the range of a value obtained by adding or subtracting half of the time width determined based on the size and moving speed of the light detection region to the period of the light detection region is a signal of the same luminescent particles. It is believed that there is.
- the signal generated within the time width determined based on the size and moving speed of the light detection region centered on the time obtained by adding the period of the light detection region to the generation time of one signal is
- the signal of the same luminescent particles can be extracted by determining that the signal is the same luminescent particle signal as the luminescent particle corresponding to one signal.
- the diffusion characteristics are separately obtained for each of the plurality of luminescent particles.
- a value or diffusion coefficient may be calculated.
- the diffusion characteristic value or the diffusion coefficient can be calculated for each.
- the moving speed of the position of the light detection region in the sample solution is based on the characteristics of the luminescent particles or the number density or concentration in the sample solution. May be changed as appropriate.
- the manner of light detected from the luminescent particles can vary depending on its properties or number density or concentration in the sample solution.
- the moving speed of the light detection region increases, the amount of light obtained from one light emitting particle decreases, so that the light from one light emitting particle can be measured with high accuracy or sensitivity.
- the moving speed is preferably changed as appropriate.
- the movement speed of the position of the light detection region in the sample solution is preferably the diffusion movement speed of the luminescent particles (the average movement speed of the particles due to Brownian motion). ) Is set higher.
- the luminescent particles are individually detected.
- a signal (representing its presence) is detected multiple times from one luminescent particle. It is difficult to make the detected signal correspond to the presence of one luminescent particle.
- the moving speed of the light detection region is set to be higher than the diffusion moving speed of the light emitting particles, so that one light emitting particle can be obtained (while the light detection region goes around a predetermined path) It is possible to correspond to one signal. Since the diffusion movement speed varies depending on the luminescent particles, it is preferable that the movement speed of the light detection region is appropriately changed according to the characteristics of the luminescent particles (particularly the diffusion characteristic value or diffusion coefficient) as described above.
- the change of the optical path of the optical system for moving the position of the light detection area may be performed by an arbitrary method.
- the position of the light detection region may be changed by changing the optical path using a galvanometer mirror employed in a laser scanning optical microscope.
- the movement path of the position of the light detection region may be arbitrarily set, and may be selected from, for example, a circle, an ellipse, a rectangle, a straight line, and a curve.
- the optical path of the optical system since the optical path of the optical system is changed to move the position of the light detection region, the light detection region can be moved quickly and in the sample solution.
- the method of the present invention is typically performed in a solution of a biological molecule such as a protein, peptide, nucleic acid, lipid, sugar chain, amino acid or aggregate thereof, or a particulate biological object such as a virus or cell.
- a biological molecule such as a protein, peptide, nucleic acid, lipid, sugar chain, amino acid or aggregate thereof, or a particulate biological object such as a virus or cell.
- a biological molecule such as a protein, peptide, nucleic acid, lipid, sugar chain, amino acid or aggregate thereof, or a particulate biological object such as a virus or cell.
- non-biological particles eg, atoms, molecules, micelles, metal colloids, etc.
- the presence of the luminescent particles is individually detected by scanning the sample solution with the photodetection region.
- the diffusion characteristic value or diffusion coefficient can be measured. Since the diffusion characteristic value or diffusion coefficient is a value reflecting the size and shape of the particles, the particle identification, the size and shape of the particles, the detection of their changes, or the measurement by the method of the present invention, or It is possible to detect and analyze various phenomena such as particle binding / dissociation reaction or dispersion / aggregation.
- the measurement of the diffusion characteristic value or the diffusion coefficient in the above-described method of the present invention is based on a novel principle, and has some characteristics different from the conventional measurement or estimation method of the diffusion coefficient.
- FCS it is possible to calculate the translational diffusion time of luminous particles dispersed and moving randomly in a sample solution, and to estimate the diffusion coefficient of luminous particles from the translational diffusion time.
- the diffusion time is a value obtained by first calculating the autocorrelation function of the fluorescence intensity during the measurement time, and the diffusion coefficient calculated therefrom is an average of a large number of luminescent particles in the sample solution. Value. Therefore, when different types of luminescent particles are present in the sample solution, the calculation process becomes complicated.
- a luminescent probe is added to the particles to be observed, and the particles are measured by FCS.
- a purification process may be necessary to remove the luminescent probe that is not bound to the particles to be observed.
- the concentration of the luminescent particles in the sample solution is a level at which one or more luminescent particles are always present during the measurement time. Need to be.
- the method of the present invention it is possible to individually detect the presence and position of luminescent particles and calculate individual diffusion characteristic values or diffusion coefficients for each particle.
- the concentration of the luminescent particles in the sample solution that can be measured with good accuracy may be much lower than in the case of FCS, and if the type of luminescent particles can be identified by the signal characteristics, etc.
- the presence or absence of different types of luminescent particles in the sample solution does not greatly affect the difficulty of calculating the diffusion characteristic value or diffusion coefficient, so even when measuring particles with a luminescent probe, There is an advantage that the removal of the luminescent probe is not essential.
- Non-Patent Document 5 As another example that can calculate the diffusion coefficient of particles in the prior art, according to SMT (Single Molecule tracking) and RICS (Raster Imaging Correlation Spectroscopy) (Non-Patent Document 5), an image taken under an optical microscope is used. However, it is possible to track the Brownian motion of luminescent particles in solution and estimate the diffusion coefficient from the motion of the two-dimensional particles. In these cases, analysis of the two-dimensional data (image data) is required. In addition, it is difficult to capture the fast movement of the luminescent particles (the time resolution of the particle displacement is limited by the video rate, for example). On the other hand, in the method of the present invention, the diffusion characteristic value or the diffusion coefficient is calculated using one-dimensional data (time-series light intensity data). The computational load is light, and the diffusion coefficient of fast-moving particles (as compared to SMT and RICS) is adjusted for each luminescent particle by appropriately adjusting the moving speed of the light detection region and / or the path length.
- the conventional method for measuring or estimating the diffusion characteristic value or diffusion coefficient there are several advantages not available.
- the luminescent particles are individually detected, the luminescent particles have a relatively low concentration in the sample solution, and the light is emitted from other luminescent particles in the conventional method. Even luminescent particles that are buried in light can be detected and their diffusion characteristic values or diffusion coefficients can be measured.
- FIG. 1A is a schematic diagram of the internal structure of an optical analyzer that performs the method of the present invention.
- FIG. 1B is a schematic diagram of a confocal volume (observation region of a confocal microscope).
- FIG. 1C is a schematic diagram of a mechanism for changing the direction of the mirror 7 to move the position of the light detection region in the sample solution.
- FIGS. 2A and 2B are a schematic diagram illustrating the principle of light detection in the scanning molecule counting method constituting a part of the method of the present invention, and a schematic diagram of a time change of measured light intensity, respectively.
- FIG. FIG. 3 is a diagram for explaining the principle of measurement of the diffusion coefficient of luminescent particles by the method of the present invention.
- (A) is a schematic perspective view of a spatial region included by movement along a predetermined path in the sample solution in the light detection region CV of the microscope.
- (B) is a graph schematically showing the intensity of light from the luminescent particles detected when the luminescent particles hardly move when the photodetection region circulates through a predetermined path with respect to time.
- (C) is a schematic perspective view of a part of the passage space region of the light detection region CV that circulates through a predetermined path, and shows the relationship between the moving distance of the luminescent particles and the time at which the luminescent particles are detected. Yes.
- FIG. 4 is a flowchart showing the procedure of the diffusion coefficient measurement executed according to the method of the present invention.
- 5A and 5B show the case where the luminescent particles traverse the light detection region while performing Brownian motion, and the position of the light detection region in the sample solution at a speed faster than the diffusion movement speed of the luminescent particles. It is a model figure showing the mode of movement of particles when luminous particles cross a photodetection region by moving.
- FIG. 6A shows a signal processing process of a detection signal in a processing procedure for detecting the presence of luminescent particles from measured time-series light intensity data (time change of photon count) according to the scanning molecule counting method. It is a figure explaining an example.
- FIG. 6B shows an actual measurement example (bar graph) of measured photon count data, a curve (dotted line) obtained by smoothing the data, and a Gaussian function (solid line) fitted in the pulse existence region. Yes. In the figure, a signal labeled “noise” is ignored as it is a signal due to noise or foreign matter.
- FIG. 6B shows an actual measurement example (bar graph) of measured photon count data, a curve (dotted line) obtained by smoothing the data, and a Gaussian function (solid line) fitted in the pulse existence region.
- a signal labeled “noise” is ignored as it is a signal due to noise or foreign matter.
- FIG. 7A is a diagram for explaining an example of processing for extracting the same luminescent particle ( ⁇ or ⁇ ) signal from the luminescent particle signals detected on the time-series light intensity data.
- FIG. 7B is a diagram for explaining the generation time intervals of the signals referred to in the extracted signals ((i) to (v)) of the same luminescent particles.
- FIG. 7A is a diagram for explaining an example of processing for extracting the same luminescent particle ( ⁇ or ⁇ ) signal from the luminescent particle signals detected on the time-series light intensity data.
- FIG. 7B is a diagram for explaining the generation time intervals of the
- FIG. 8A is an actual measurement example of a part of time-series light intensity data (photon count data) in which a periodic strong pulse signal is observed in the first embodiment, and FIG. These show the photon count data expanded on the time axis of the signal 4 and its fitting curve.
- FIG. 8C shows the time (number of periods) of the mean square displacement of the luminescent particles calculated based on the deviation time from the movement period of the light detection region in the signal generation time interval in (A). It is a graph which shows the plot with respect to * moving period) and a fitting straight line.
- FIG. 9 shows the entire time series light intensity data (photon count data) measured in Example 1 (2 seconds).
- FIG. 10 shows an example of the time change of photon count (light intensity) obtained in the conventional optical analysis technique for calculating the fluctuation of fluorescence intensity.
- FIG. 10A shows that the concentration of particles in the sample is sufficient. This is a case where measurement accuracy is given, and (B) is a case where the concentration of particles in the sample is significantly lower than in (A).
- an optical analyzer 1 includes optical systems 2 to 17 and a computer 18 for controlling the operation of each part of the optical system and acquiring and analyzing data.
- the optical system of the optical analyzer 1 may be the same as the optical system of a normal confocal microscope, in which the laser light (Ex) emitted from the light source 2 and propagated through the single mode fiber 3 is a fiber.
- the light is emitted as a divergent light at an angle determined by a specific NA at the outgoing end of the light, becomes parallel light by the collimator 4, is reflected by the dichroic mirror 5, the reflection mirrors 6, 7, and the objective lens 8. Is incident on.
- a microplate 9 in which a sample container or well 10 into which a sample solution of 1 to several tens of ⁇ L is dispensed is typically arranged is emitted from the objective lens 8.
- the laser light is focused in the sample solution in the sample container or well 10 to form a region with high light intensity (excitation region).
- luminescent particles that are the object of observation typically molecules to which a luminescent label such as a fluorescent dye has been added are dispersed or dissolved, and when the luminescent particles enter the excitation region, they emit light during that time. The particles are excited and light is emitted.
- the emitted light (Em) passes through the objective lens 8 and the dichroic mirror 5, is reflected by the mirror 11, is collected by the condenser lens 12, and passes through the pinhole 13.
- the pinhole 13 is disposed at a position conjugate with the focal position of the objective lens 8, and is schematically shown in FIG.
- the focal region of the laser light illustrated in FIG. 1B is a light detection region in the present optical analyzer having an effective volume of about 1 to 10 fL, and is referred to as a confocal volume.
- the light intensity is typically a Gaussian or Lorentzian distribution with the center of the region at the apex, and the effective volume is bounded by the surface where the light intensity is 1 / e 2.
- the light passing through the pinhole 13 passes through the barrier filter 14 via the dichroic mirror 14a (here, only the light component in a specific wavelength band is selected) and is introduced into the multimode fiber 15.
- the light reaches the corresponding photodetector 16 and is converted into a time-series electrical signal, which is then input to the computer 18 and processed for optical analysis in a manner described later.
- the photodetector 16 an ultrasensitive photodetector that can be used for photon counting is preferably used, so that light from one luminescent particle, for example, one or several fluorescent dye molecules can be used. The weak light can be detected.
- the optical path of the optical system is further changed, and the sample solution is scanned by the light detection region, that is, the focal region (ie, the light detection in the sample solution).
- a mechanism is provided for moving the position of the region.
- a mirror deflector 17 that changes the direction of the reflection mirror 7 may be employed as schematically illustrated in FIG.
- Such a mirror deflector 17 may be the same as a galvanometer mirror device provided in a normal laser scanning microscope.
- the mirror deflector 17 is driven in cooperation with light detection by the light detector 16 under the control of the computer 18.
- the movement path of the position of the light detection region may be arbitrarily selected from a circle, an ellipse, a rectangle, a straight line, a curve, or a combination thereof (so that various movement patterns can be selected by the program in the computer 18). It may be.)
- the position of the light detection region may be moved in the vertical direction by moving the objective lens 8 up and down.
- mechanical vibrations and hydrodynamic actions are substantially caused in the sample solution. It is possible to eliminate the influence of the dynamic action on the observation object and to achieve stable measurement.
- a stage (not shown) of the microscope is provided with a stage position changing device 17a for moving the horizontal position of the microplate 9 in order to change the well 10 to be observed. Good.
- the operation of the stage position changing device 17a may be controlled by the computer 18.
- the above optical system is used as a multiphoton microscope. In that case, since there is light emission only in the focal region (light detection region) of the excitation light, the pinhole 13 may be removed.
- the optical system of the confocal microscope is used as it is.
- the optical systems 2 to 5 for generating excitation light may be omitted.
- a plurality of excitation light sources 2 may be provided, and the wavelength of the excitation light can be appropriately selected according to the wavelength of the light for exciting the luminescent particles. Good.
- a plurality of photodetectors 16 may be provided, and when a plurality of types of luminescent particles having different wavelengths are included in the sample, light from them can be detected separately according to the wavelength. It's okay.
- the position of the light detection region of the confocal microscope or the multiphoton microscope is determined in the sample solution.
- the movement of the photodetection area in the interval of the generation time of the signal of the luminescent particles that reflects the deviation of the position of the luminescent particles while the photodetection area goes around the predetermined path Based on the deviation time from the cycle, the ease of movement of the luminescent particles, that is, the diffusion characteristic value or diffusion coefficient of the luminescent particles is calculated. According to this configuration, each of the luminescent particles in the sample solution is individually detected, and the diffusion characteristic value or diffusion coefficient is individually measured.
- the concentration of the luminescent particles in the sample solution is a fluorescence such as FCS. Even when the concentration is lower than a concentration that can be measured satisfactorily by a spectroscopic technique that requires statistical processing for calculating the magnitude of fluctuation, the diffusion characteristic value or diffusion coefficient of the luminescent particles can be measured.
- FCS fluorescence
- the principle of the scanning molecule counting method and the method of measuring the diffusion characteristic value or diffusion coefficient according to the present invention will be described.
- the spectroscopic analysis technology such as FCS is superior to the conventional biochemical analysis technology in that the required amount of sample is extremely small and the inspection can be performed quickly.
- the characteristics of the luminescent particles are calculated based on fluctuations in fluorescence intensity. Therefore, in order to obtain accurate measurement results, the luminescent particles in the sample solution
- the concentration or number density is a level at which about one luminescent particle is always present in the light detection region CV during the measurement of the fluorescence intensity. As shown on the right side, it is required that a significant light intensity (photon count) is always detected during the measurement time.
- the concentration or number density of the luminescent particles is lower than that, for example, as shown in FIG. 10B, if the luminescent particles are at a level that only occasionally enters the light detection region CV, As illustrated on the right side of the figure, a significant light intensity signal (photon count) appears only during a part of the measurement time, making it difficult to accurately calculate the fluctuation of the light intensity. Also, if the concentration of the luminescent particles is much lower than the level at which about one luminescent particle is always present in the light detection area during measurement, the light intensity fluctuation is affected by the background. The measurement time is long in order to easily obtain significant light intensity data sufficient for calculation.
- a mechanism (mirror deflector 17) for moving the position of the light detection region is driven to change the optical path, and the pattern is schematically shown in FIG.
- the light detection is performed while moving the position of the light detection region CV in the sample solution, that is, while scanning the sample solution by the light detection region CV.
- FIG. 2A when the light detection region CV moves (time to to t2 in the figure) and passes through the region where one luminescent particle exists (t1), Light is emitted from the luminescent particles, and a pulse-like signal having a significant light intensity (Em) appears on the time-series light intensity data as illustrated in FIG.
- the movement of the position of the light detection region CV and the light detection are executed, and pulse-like signals (significant light intensity) appearing in the meantime illustrated in FIG. 2B are detected one by one.
- the luminescent particles are individually detected, and information on the characteristics of the luminescent particles can be acquired.
- statistical calculation processing such as calculation of fluctuations in fluorescence intensity is not performed, and luminescent particles are detected one by one. Therefore, FCS, FIDA, etc. have sufficient accuracy. Information on the characteristics of the particles can be obtained even in a sample solution in which the concentration of particles to be observed is so low that analysis is impossible.
- the light detection region (CV) is in the sample solution. , It is circulated so as to pass through a predetermined path (for example, a ring with a radius R). During the movement of the light detection region, the position of the luminescent particles moves due to Brownian motion. Once the luminescent particles are detected (the luminescent particles once included in the light detection region), the light detection region has a predetermined path. If the light detection region does not deviate from the space region through which the light detection region passes, the luminescent particles will be detected again.
- a predetermined path for example, a ring with a radius R.
- the luminescent particles once detected will be included in the light detection region for each lap of the light detection region for a while.
- the signal representing the light of the luminescent particles is approximately periodic every time (movement period) tcycle in which the light detection region makes a round of a predetermined path. Detected.
- the interval between the generation times of such periodically detected signals does not completely coincide with the movement period of the light detection region, and the emission particles in the time that the light detection region circulates a predetermined path Depending on the movement of the position due to the Brownian motion, the movement period of the light detection region increases or decreases, that is, a time difference from the movement period occurs. Therefore, in the present invention, the movement of the luminescent particles due to the Brownian motion is facilitated based on the deviation time from the movement period of the light detection region in the generation time interval of the periodically detected signal. That is, the calculation of the diffusion characteristic value is attempted.
- the relationship between the particle diffusion coefficient D and the one-dimensional displacement x (t) of the particle at time t is obtained from the Einstein-Smoluchowski equation.
- ⁇ X (t)> 2 2Dt (5)
- the displacement x (k ⁇ of the position of the luminescent particle from the shift time from the moving period of the light detection region in the generation time interval of the periodically detected signal. tcycle) is calculated, and the diffusion coefficient D can be calculated from the displacement using Equation (5).
- the diffusion coefficient is so large that the (average) displacement of the luminescent particles in the movement cycle tcycle of a certain photodetection region exceeds the size of the photodetection region, the signal of the same luminescent particle is photodetected. It becomes difficult to capture every round of the area. This is because, if the moving direction of the luminescent particles once included in the light detection region coincides with the passage region (predetermined path) of the light detection region, it is included again in the light detection region after the circulation of the light detection region.
- the luminescent particles move in a random direction, when the (average) displacement of the luminescent particles in one period of the photodetection region is large enough to exceed the size of the photodetection region, the luminescent particles are This is because, after being included by the light detection region, it is highly likely that the light detection region deviates from the passage region of the light detection region and is not included again by the light detection region after the light detection region circulates. Therefore, in order to capture a periodic signal in order to reliably achieve the above calculation of the diffusion coefficient D, as illustrated in FIG. 3D, the luminescent particles in the light detection region moving period tcycle are illustrated.
- the moving period tcycle of the light detection region may be adjusted so that the condition of the above formula (6) is satisfied in the expected diffusion coefficient of the luminescent particles to be inspected. .
- FIG. 4 shows a processing process in the present embodiment expressed in the form of a flowchart.
- the particles to be observed are any molecules such as dissolved molecules that are dispersed in the sample solution and randomly move in the solution.
- biomolecules such as proteins, peptides, nucleic acids, lipids, sugar chains, amino acids or aggregates thereof, viruses, cells, metal colloids, other non-biological particles, etc.
- the sample solution is typically an aqueous solution, but is not limited thereto, and may be an organic solvent or any other liquid.
- the particles to be observed may be particles that emit light themselves, or particles to which a light-emitting label (fluorescent molecule, phosphorescent molecule, chemical / bioluminescent molecule) is added in any manner. It may be.
- the mirror deflector 17 is driven to move the position of the light detection region in the sample solution (sample The light intensity is measured while scanning the solution) (step 100 in FIG. 4).
- the operation process typically, after injecting the sample solution into the well 10 of the microplate 9 and placing it on the stage of the microscope, the user inputs an instruction to start measurement to the computer 18. Then, the computer 18 stores a program stored in a storage device (not shown) (a procedure for changing the optical path to move the position of the light detection region in the sample solution, and during the movement of the position of the light detection region).
- irradiation of excitation light and measurement of light intensity in the light detection region in the sample solution are started.
- the measurement is started, first, under the control of the processing operation according to the program of the computer 18, light having the excitation wavelength of the luminescent particles in the sample solution is emitted from the light source 2, and the mirror deflector 17 is moved to the mirror 7. (Galvano mirror) is driven to move the position of the light detection region in the well 10, and at the same time, the light detector 16 sequentially converts the detected light into an electric signal to generate a computer.
- the computer 18 generates and stores time-series light intensity data from the transmitted signal in an arbitrary manner.
- the photodetector 16 is an ultra-sensitive photodetector that can detect the arrival of one photon, so that the light detection is performed sequentially over a predetermined period of time at a predetermined unit time (BIN).
- TIME for example, photon counting executed in a mode in which the number of photons arriving at the photodetector every 10 ⁇ sec is measured, and the time-series light intensity data is time-series photon count data. It's okay.
- the moving speed of the position of the light detection region in the scanning molecule counting method, in general, in order to carry out individual detection of luminescent particles from measured time-series light intensity data quantitatively and accurately.
- the moving speed of the position of the light detection region during the measurement of the light intensity is set to a value faster than the random movement of the luminescent particles, that is, the moving speed due to the Brownian movement.
- the moving speed of the position of the light detection region is slower than the movement of the particle due to Brownian motion, the particle moves randomly within the region as schematically illustrated in FIG.
- the light intensity changes randomly (the excitation light intensity in the light detection region decreases outward with the center of the region as the apex), and significant light intensity changes corresponding to individual light emitting particles (light emission) It is difficult to specify the signal representing the light from the particles. Therefore, preferably, as shown in FIG. 5B, the particles cross the light detection region in a substantially straight line, so that the light intensity corresponding to each particle in the time-series light intensity data. (When the particle passes through the light detection region substantially linearly, the profile of the light intensity change is substantially the same as the excitation light intensity distribution. See the upper part of FIG. 6A.) .), So that the correspondence between individual luminous particles and light intensity can be easily identified, the moving speed of the position of the light detection region is set faster than the average moving speed (diffuse moving speed) due to the Brownian motion of the particles.
- the moving speed of the position of the light detection region may be set to 15 mm / s, which is 10 times or more.
- various movement speeds of the position of the light detection region are set, and the profile of the change in light intensity is expected to be expected (typically, the excitation light intensity distribution and Preliminary experiments for finding conditions that are substantially the same) may be repeatedly performed to determine a suitable moving speed of the position of the light detection region.
- the moving period tcycle of the light detection region is preferably set to satisfy the condition of the expression (6) with respect to the diffusion coefficient D of the luminescent particles to be inspected.
- the locus of one luminescent particle passing through the light detection region is a substantially straight line as shown in FIG.
- the light intensity change in the signal corresponding to the particle has a substantially bell-shaped profile reflecting the light intensity distribution in the light detection region (determined by the optical system) (FIG. 8 ( B)). Therefore, in the scanning molecule counting method, basically, when the time duration in which the light intensity exceeds an appropriately set threshold value is within a predetermined range, a signal having the light intensity profile is detected as one particle. It may be determined that it corresponds to having passed through the region, and one luminescent particle may be detected.
- a signal whose duration of light intensity exceeding the threshold is not within a predetermined range is determined as a noise or foreign matter signal.
- the intensity A and the width a calculated by fitting the expression (12) to a significant light intensity profile are within a predetermined range.
- a signal whose intensity A and width a are outside the predetermined range is determined as a noise or foreign object signal and may be ignored in the subsequent analysis or the like.
- time-series light intensity data (FIG. 6A, uppermost “detection result (unprocessed)”)
- a smoothing (smoothing) process is performed (step 110 in FIG. 4 and “smoothing” in FIG. 6A).
- the light emitted by the luminescent particles is probabilistically emitted, and data values may be lost in a very short time. Therefore, such a data value loss can be ignored by the smoothing process.
- the smoothing process may be performed by, for example, a moving average method.
- the parameters for executing the smoothing process are the moving speed (scanning of the position of the light detection region at the time of acquiring the light intensity data).
- Speed scanning of the position of the light detection region at the time of acquiring the light intensity data.
- BIN TIME may be set as appropriate.
- a smoothing process is performed in order to detect a time region (pulse existing region) where a significant pulse-like signal (hereinafter referred to as “pulse signal”) exists.
- a first derivative value with respect to time of the subsequent time-series light intensity data is calculated (step 120).
- the time differential value of the time-series light intensity data has a large change in value at the time of change of the signal value, as illustrated in the lower “time differential” in FIG. By reference, significant signal start and end points can be advantageously determined.
- a significant pulse signal is sequentially detected on the time-series light intensity data, and it is determined whether or not the detected signal is a signal corresponding to the luminescent particles. Specifically, first, on the time-series time differential value data of the time-series light intensity data, with reference to the time differential value sequentially, the start point and the end point of one pulse signal are searched and determined, A pulse presence region is identified (step 130). When one pulse existence area is specified, a bell-shaped function fitting is performed on the smoothed time-series light intensity data in the pulse existence area (see FIG. 6A, “bottom-shaped function fitting”).
- the bell-shaped function to be fitted is typically a Gaussian function, but may be a Lorentz-type function. Whether or not the calculated bell-shaped function parameter is within a range assumed for the bell-shaped profile parameter drawn by the pulse signal detected when one luminescent particle passes through the light detection region, That is, it is determined whether or not the peak intensity, pulse width, and correlation coefficient of the pulse are within predetermined ranges (step 150). Thus, as shown on the left of FIG.
- the signal for which the calculated bell-shaped function parameter is determined to be within the range assumed in the signal corresponding to one luminescent particle is It is determined that the signal corresponds to one luminescent particle, and thus one luminescent particle is detected.
- a pulse signal whose calculated bell-shaped function parameter is not within the assumed range is ignored as noise.
- the search and determination of the pulse signal in the processing of steps 130 to 150 may be repeatedly executed over the entire area of the time-series light intensity data (step 160).
- grains individually from time series light intensity data is not restricted to said procedure, You may perform with arbitrary methods.
- the light detection region of the luminescent particles to be observed is detected.
- the movement period of the light detection region is adjusted so that the (average) displacement for each turn does not exceed the size of the light detection region (see Expression (6)).
- it is expected that the position of the luminescent particles once detected after the light detection region circulates is within the range of the light detection region. That is, it corresponds to the time for the light detection region to move in the direction of movement (diameter d) around the time when one period of the light detection region elapses after the signal of a certain luminescent particle is detected.
- Time span, ⁇ T d / v (2) It can be determined that the signal generated within the range is the signal of the same luminescent particle as the one luminescent particle. Therefore, as one of the methods for extracting the signal of the same luminescent particle, the time centered on the time obtained by adding the period of the light detection region to the generation time of the signal of one luminescent particle in the time-series light intensity data.
- An algorithm may be employed that determines that the signal generated within the range of the width ⁇ T is a signal of the same luminescent particles as the luminescent particles corresponding to the one signal. More specifically, as schematically illustrated in FIG.
- the generation time of the selected signal ⁇ (i) If the signal ⁇ (ii) exists within the time width ⁇ T centering on the time when one period of the light detection region elapses from (may be at the peak time), the signal ⁇ (ii) It is selected as the signal of the same luminescent particles as the signal ⁇ (i). After that, when the signal ⁇ (iii) exists within the range of the time width ⁇ T, centering on the time (tcycle) in which one cycle of the light detection region elapses from the generation time of the selected signal ⁇ (ii).
- the signal ⁇ (iii) is selected as the signal of the same luminescent particles as the already selected signals ⁇ (i) and ⁇ (ii).
- the process of selecting a signal existing within the time width ⁇ T around the time when one cycle of the light detection region elapses from the generation time of the selected signal is sequentially performed.
- the process of selecting a signal existing within the time width ⁇ T around the time when one cycle of the light detection region elapses from the generation time of the selected signal is sequentially performed.
- a series of signals (signal group) of the same luminescent particles on the time-series light intensity data By executing it repeatedly, it is possible to extract a series of signals (signal group) of the same luminescent particles on the time-series light intensity data.
- the signal may be selected as the same luminescent particle signal as the initially selected signal.
- the signals may be sequentially extracted in accordance with an algorithm similar to the above for another group of signals that appear periodically. That is, when there are a plurality of periodically appearing signal groups on one time-series light intensity data, each of these signal groups may be extracted individually. In the actual extraction process, for example, in the time-series light intensity data, a certain signal ( ⁇ (i)) is first selected, and the above-described mode is set based on the one signal.
- one periodic signal group ( ⁇ as described above is used as a reference with one signal ( ⁇ (i)) not selected as the extracted signal group. (Ii), ...) are extracted.
- a plurality of signal groups may be extracted on one time-series light intensity data by repeating such processing.
- the signal extraction method described above is advantageously used when a signal is automatically extracted by a computer, particularly when a large number of luminescent particle signals are detected on the time-series light intensity data.
- D x 2 / (2 k ⁇ tcycle) (14)
- the average value of the calculated D may be used as the diffusion coefficient of the luminescent particles.
- the “diffusion coefficient” of the luminescent particles can be “measured”.
- the diffusion coefficient is a physical quantity that reflects the size and shape of the particle. Therefore, according to the present invention, a luminescent particle can be formed by attaching a luminescent label to any particle to be observed, and the diffusion of such luminescent particles By measuring the coefficient, it is possible to obtain information on the size, structure or change of particles to be observed, or various intermolecular interactions.
- the size of the measurable diffusion coefficient can be varied, so that the diffusion coefficient of a relatively wide range of types of particles can be measured. It is expected.
- the movement period of the light detection region may be adjusted to an appropriate value through preliminary experiments so that a periodic signal of the observation target particles can be obtained.
- SYTOX Orange (Invitrogen, Cat. No. S-11368) is contained in a phosphate buffer solution (containing 0.05% Tween 20) at 10 nM. A solution was prepared by dissolving Cat. No. 3035) to a concentration of 1 pM. SYTOX Orange is a fluorescent dye whose fluorescence intensity increases by about 500 times when bound to DNA (plasmid).
- a single molecule fluorescence measurement device MF20 (Olympus Corporation) equipped with an optical system of a confocal fluorescence microscope and a photon counting system is used as an optical analysis device, and the above-mentioned “(2) Sample solution
- time-series light intensity data photon count data
- laser light of 633 nm was used as excitation light
- light in a wavelength band of 660 to 710 nm was measured using a bandpass filter to generate time-series light intensity data.
- the light detection region in the sample solution was circulated through a circular path (radius of about 23.9 ⁇ m) with a moving period of 10 msec and a moving speed of 15 mm / sec.
- BIN TIME was set to 10 ⁇ s, and the measurement time was set to 2 seconds.
- the time-series light intensity data obtained for the sample solution is subjected to smoothing according to the processing procedure described in “(3) (i) Detection of signal corresponding to luminescent particles” above, and smoothing is performed.
- FIG. 8A shows about (in the light detection region moving period) about 1.5 to 1.6 seconds of light intensity data obtained by the measurement for 2 seconds.
- Examples of signals 1 to 5 representing light of luminescent particles periodically appearing in 10 milliseconds are shown.
- the peak time of each signal was as follows (unit: m seconds).
- Signal 1 1510.741 Signal 2 1520.726 Signal 3 1530.738 Signal 4 1540.767 Signal 5 1550.825 The period of these series of signals is substantially equal to the movement period of the light detection region, and is considered to be the signal of the same luminescent particles.
- FIG. 8C is a diagram in which the square value (x 2 ) of the displacement x thus calculated is plotted against time (number of periods ⁇ movement period) (each point represents the displacement x at each time).
- Equation (5) is established between the average value and time of the square value x 2 of the displacement x, the in moving cycle interval of generation time of the signal It shows that the displacement x of the luminescent particles due to the Brownian motion can be calculated based on the deviation time ⁇ t from the corresponding time.
- the signal sets corresponding to the same luminescent particles are separately extracted from the luminescent particle signals in the whole light intensity data (FIG. 9) obtained by the above-mentioned measurement for 2 seconds, and the luminescent particles The diffusion coefficient was calculated every time.
- the signal extraction as described in “(ii) Extracting the signal of the same luminescent particle”, the signal of one luminescent particle is first selected on the light intensity data, and then the selected signal is selected.
- the signal existing within the range of the time ⁇ T given by the equation (2) around the time when the movement period (tcycle) of the light detection region elapses from the peak occurrence time of the detected signal is the same as the first selected signal.
- the total number of luminescent particle signals on the light intensity data in FIG. 9 is 7188, and the number of signal sets generated periodically two or more times for each movement period of the light detection region is 1708. (The total number of signals generated periodically was 4156). Since the number of such signal pairs corresponds to the number of luminescent particles, in this embodiment, the value of the diffusion coefficient of 1708 luminescent particles can be obtained in one light intensity data measured for 2 seconds. It will be. Thus, for each of the detected 1708 signal groups, a deviation time ⁇ t from the time corresponding to the movement period in the signal generation time interval is calculated according to the equation (13), and then according to the equation (4).
- the theoretically calculated diffusion coefficient is 4.2 ⁇ 10 ⁇ 12 [m 2 / s] (correction according to Equation (8) of Non-Patent Document 6 was used).
- the result value of the diffusion coefficient in the above-mentioned embodiment is almost in order with the FCS method and the theoretical value, which means that the particle diffusion coefficient can be measured by the method of the present invention. It is shown that.
- the diffusion coefficient of the luminescent particles can be measured by the scanning molecule counting method.
- the method of the present invention is configured to individually detect periodically generated luminescent particle signals in the light intensity data and calculate the diffusion coefficient based on the generation time of the signals.
- it is possible to measure the diffusion coefficient of luminescent particles even when the concentration of luminescent particles in the sample solution is lower than the concentration range required by optical analysis techniques such as FCS. May be advantageous when analyzing rare or expensive samples often used in the field of medical and biological research and development.
- the diffusion coefficient is calculated based on the generation time of the signal of the periodically generated luminescent particles on the one-dimensional light intensity data, the calculation load is relatively low, and one It is expected that the calculation amount or time required for calculating the diffusion coefficient of the luminescent particles is reduced as compared with a method using image processing such as SMT.
- the diffusion coefficient is measured using the scanning molecule counting method.
- the time difference from the movement period of the light detection region in the signal generation time interval is described.
- a diffusion characteristic value other than the diffusion coefficient can be calculated based on this, and such a case also belongs to the scope of the present invention.
- the slope of the plot of the square value of the displacement of the particle with respect to the time calculated based on “the shift time from the movement period of the light detection region in the signal generation time interval”, the translational diffusion time is calculated, It may be used for evaluation of the size or structural change of and the interaction between molecules.
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Abstract
Description
<x(t)>2=2Dt …(1)
(ここで、<x(t)>は、変位の平均)
により定義されるので、かかる式(1)の関係を用いて、発光粒子の変位に基づいて算定されてよい。
ΔT=d/v …(2)
により与えられてよい。
2…光源
3…シングルモードオプティカルファイバー
4…コリメータレンズ
5…ダイクロイックミラー
6、7、11…反射ミラー
8…対物レンズ
9…マイクロプレート
10…ウェル(試料溶液容器)
12…コンデンサーレンズ
13…ピンホール
14…バリアフィルター
15…マルチモードオプティカルファイバー
16…光検出器
17…ミラー偏向器
17a…ステージ位置変更装置
18…コンピュータ
本発明による方法は、基本的な構成に於いて、図1(A)に模式的に例示されている如き、FCS、FIDA等が実行可能な共焦点顕微鏡の光学系と光検出器とを組み合わせてなる光分析装置により実現可能である。図1(A)を参照して、光分析装置1は、光学系2~17と、光学系の各部の作動を制御すると共にデータを取得し解析するためのコンピュータ18とから構成される。光分析装置1の光学系は、通常の共焦点顕微鏡の光学系と同様であってよく、そこに於いて、光源2から放射されシングルモードファイバー3内を伝播したレーザー光(Ex)が、ファイバーの出射端に於いて固有のNAにて決まった角度にて発散する光となって放射され、コリメーター4によって平行光となり、ダイクロイックミラー5、反射ミラー6、7にて反射され、対物レンズ8へ入射される。対物レンズ8の上方には、典型的には、1~数十μLの試料溶液が分注される試料容器又はウェル10が配列されたマイクロプレート9が配置されており、対物レンズ8から出射したレーザー光は、試料容器又はウェル10内の試料溶液中で焦点を結び、光強度の強い領域(励起領域)が形成される。試料溶液中には、観測対象物である発光粒子、典型的には、蛍光色素等の発光標識が付加された分子が分散又は溶解されており、発光粒子が励起領域に進入すると、その間、発光粒子が励起され光が放出される。放出された光(Em)は、対物レンズ8、ダイクロイックミラー5を通過し、ミラー11にて反射してコンデンサーレンズ12にて集光され、ピンホール13を通過する。なお、当業者に於いて知られている如く、ピンホール13は、対物レンズ8の焦点位置と共役の位置に配置されており、これにより、図1(B)に模式的に示されている如きレーザー光の焦点領域、即ち、励起領域内から発せられた光のみがピンホール13を通過し、焦点面以外からの光は遮断される。図1(B)に例示されたレーザー光の焦点領域は、通常、1~10fL程度の実効体積を有する本光分析装置に於ける光検出領域であり、コンフォーカル・ボリュームと称される。コンフォーカル・ボリュームに於いては、典型的には、光強度が領域の中心を頂点とするガウス型分布又はローレンツ型分布となり、その実効体積は、光強度が1/e2となる面を境界とする略楕円球体の体積である。かくして、ピンホール13を通過した光は、ダイクロイックミラー14aを経て、バリアフィルター14を透過して(ここで、特定の波長帯域の光成分のみが選択される。)、マルチモードファイバー15に導入されて、対応する光検出器16に到達し、時系列の電気信号に変換された後、コンピュータ18へ入力され、後に説明される態様にて光分析のための処理が為される。光検出器16としては、好適には、フォトンカウンティングに使用可能な超高感度の光検出器が用いられ、これにより、1つの発光粒子からの光、例えば、一個又は数個の蛍光色素分子からの微弱光が検出可能となる。
「発明の概要」の欄に記載されている如く、本発明の方法によれば、端的に述べれば、共焦点顕微鏡又は多光子顕微鏡の光検出領域の位置を試料溶液内にて所定の経路に沿って周期的に移動しながら、試料溶液中にて分散する発光粒子が光検出領域内に包含される際に放出する光を検出して発光粒子の存在を個別に検知する「走査分子計数法」に於いて、光検出領域が所定の経路を周回する間の発光粒子の位置のずれが反映される発光粒子の信号の発生時間の間隔に於ける光検出領域の移動周期からのずれ時間に基づいてその発光粒子の移動し易さ、即ち、発光粒子の拡散特性値又は拡散係数が算出される。かかる構成によれば、試料溶液中の発光粒子の各々を個別に検出し、その拡散特性値又は拡散係数が個別に測定されることになるので、試料溶液中の発光粒子濃度がFCS等の蛍光ゆらぎの大きさの算出のための統計的処理を要する分光分析技術で良好に測定可能な濃度よりも低い場合でも、発光粒子の拡散特性値又は拡散係数の測定が可能となる。以下、走査分子計数法及び本発明による拡散特性値又は拡散係数の測定方法の原理について説明する。
FCS等の分光分析技術は、従前の生化学的な分析技術に比して、必要な試料量が極めて少なく、且つ、迅速に検査が実行できる点で優れている。しかしながら、FCS等の分光分析技術では、原理的に、発光粒子の特性は、蛍光強度のゆらぎに基づいて算定されるので、精度のよい測定結果を得るためには、試料溶液中の発光粒子の濃度又は数密度が、図10(A)に模式的に描かれているように、蛍光強度の計測中に常に一個程度の発光粒子が光検出領域CV内に存在するレベルであり、同図の右側に示されている如く、計測時間中に常に有意な光強度(フォトンカウント)が検出されることが要求される。もし発光粒子の濃度又は数密度がそれよりも低い場合、例えば、図10(B)に描かれているように、発光粒子がたまにしか光検出領域CV内へ進入しないレベルである場合には、同図の右側に例示されている如く、有意な光強度の信号(フォトンカウント)が、計測時間の一部にしか現れないこととなり、精度のよい光強度のゆらぎの算定が困難となる。また、計測中に常に一個程度の発光粒子が光検出領域内に存在するレベルよりも発光粒子の濃度が大幅に低い場合には、光強度のゆらぎの演算に於いて、バックグラウンドの影響を受けやすく、演算に十分な量の有意な光強度データを得るために計測時間が長くなる。
上記の走査分子計数法に於いて、図3(A)に模式的に描かれている如く、光検出領域(CV)は、試料溶液中で、所定の経路(例えば、半径Rの円環)を通過するよう循環される。かかる光検出領域の移動の間、発光粒子は、ブラウン運動により、位置が移動するところ、一度検出された発光粒子(一度光検出領域に包含された発光粒子)が、光検出領域が所定の経路を周回する間に、光検出領域の通過する空間領域から逸脱しない場合には、その発光粒子は再び検出されることとなる。特に、発光粒子のブラウン運動の速度が比較的低いときには、一度検出された発光粒子は、しばらくの間、光検出領域の周回毎に光検出領域に包含されることとなるので、光強度データ上に於いて、発光粒子の光を表す信号が、図3(B)に模式的に例示されている如く、概ね、光検出領域が所定の経路を一周する時間(移動周期)tcycle毎に周期的に検出される。しかしながら、かかる周期的に検出される信号の発生時間の間隔は、光検出領域の移動周期に完全には一致しておらず、光検出領域が所定の経路を周回する時間に於ける発光粒子のブラウン運動による位置の移動に依存して光検出領域の移動周期に対して増減する、即ち、移動周期からのずれ時間が生ずることとなる。そこで、本発明に於いては、上記の周期的に検出される信号の発生時間の間隔に於ける光検出領域の移動周期からのずれ時間に基づいて、発光粒子のブラウン運動による移動のし易さ、即ち、拡散特性値の算出が試みられる。なお、以下、拡散特性値として、拡散係数を算出する例について説明されるが、その他の拡散特性値についても信号の発生時間の間隔に於ける移動周期からのずれ時間に基づいて適宜算出できることは理解されるべきであり、そのような場合も本発明の範囲に属することは理解されるべきである。
t=tk=to+k・tcycle+Δt …(3)
により与えられる。ここで、Δtは、発光粒子の位置の移動による発光粒子が光検出領域に包含される時刻のずれ、即ち、二つの信号の発生時間の間隔に於ける光検出領域の移動周期からのずれ時間である(Δtは、正又は負で有り得る。)。従って、k・tcycle(k周期)に於ける光検出領域の移動方向に沿った発光粒子の位置の変位x(k・tcycle)は、光検出領域の移動速度vを用いて、
x(k・tcycle)=vΔt …(4)
により与えられる。ところで、粒子の拡散係数Dと時間tに於ける粒子の一次元の変位x(t)の関係は、アインシュタイン-スモルコフスキー(Einstein-Smoluchowski)の式より、
<x(t)>2=2Dt …(5)
により与えられる。かくして、式(3)、(4)を用いて、周期的に検出される信号の発生時間の間隔に於ける光検出領域の移動周期からのずれ時間から発光粒子の位置の変位x(k・tcycle)を算出し、それらの変位を用いて、式(5)により、拡散係数Dを算定することが可能となる。
(2r)2>2δ・D・tcycle …(6)
(ここで、δは、次元であり、ここでは、δ=3である。)
を満たすよう調整される。なお、実際の測定に於いては、検査されるべき発光粒子の予想される拡散係数に於いて、上記の式(6)の条件が成立するよう光検出領域の移動周期tcycleが調整されてよい。
図1(A)に例示の光分析装置1を用いた本発明の方法による発光粒子の拡散係数の測定方法の実施形態に於いては、具体的には、(1)発光粒子を含む試料溶液の調製過程、(2)試料溶液の光強度の測定処理過程、及び(3)測定された光強度の分析処理過程が実行される。図4は、フローチャートの形式にて表した本実施形態に於ける処理過程を示している。
本発明の方法に於いて観測対象となる粒子は、溶解された分子等の、試料溶液中にて分散し溶液中にてランダムに運動する粒子であれば、任意のものであってよく、例えば、タンパク質、ペプチド、核酸、脂質、糖鎖、アミノ酸若しくはこれらの凝集体などの生体分子、ウイルス、細胞、或いは、金属コロイド、その他の非生物学的粒子などであってよい(試料溶液は、典型的には水溶液であるが、これに限定されず、有機溶媒その他の任意の液体であってよい。)。また、観測対象となる粒子は、それ自体が発光する粒子であってもよく、或いは、発光標識(蛍光分子、りん光分子、化学・生物発光分子)が任意の態様にて付加された粒子であってよい。
本実施形態の走査分子計数法による光強度の測定処理過程では、ミラー偏向器17を駆動して、試料溶液内での光検出領域の位置の移動(試料溶液内の走査)を行いながら、光強度の測定が為される(図4-ステップ100)。操作処理に於いて、典型的には、マイクロプレート9のウェル10に試料溶液を注入して顕微鏡のステージ上に載置した後、使用者がコンピュータ18に対して、測定の開始の指示を入力すると、コンピュータ18は、記憶装置(図示せず)に記憶されたプログラム(試料溶液内に於いて光検出領域の位置を移動するべく光路を変更する手順と、光検出領域の位置の移動中に光検出領域からの光を検出する手順)に従って、試料溶液内の光検出領域に於ける励起光の照射及び光強度の計測が開始される。計測が開始されると、まず、コンピュータ18のプログラムに従った処理動作の制御下、光源2から、試料溶液中の発光粒子の励起波長の光が出射されると共に、ミラー偏向器17がミラー7(ガルバノミラー)を駆動して、ウェル10内に於いて光検出領域の位置の移動を実行し、これと同時に光検出器16は、逐次的に検出された光を電気信号に変換してコンピュータ18へ送信し、コンピュータ18は、任意の態様にて、送信された信号から時系列の光強度データを生成して保存する。典型的には、光検出器16は、一光子の到来を検出できる超高感度光検出器であるので、光の検出は、所定時間に亘って、逐次的に、所定の単位時間毎(BIN TIME)に、例えば、10μ秒毎に光検出器に到来するフォトンの数を計測する態様にて実行されるフォトンカウンティングであり、時系列の光強度のデータは、時系列のフォトンカウントデータであってよい。
(2r)2=6D・Δτ …(7)
から、
Δτ=(2r)2/6D …(8)
となるので、発光粒子がブラウン運動により移動する速度(拡散移動速度)Vdifは、概ね、
Vdif=2r/Δτ=3D/r …(9)
となる。そこで、光検出領域の位置の移動速度は、かかるVdifを参照して、それよりも十分に早い値に設定されてよい。例えば、発光粒子の拡散係数が、D=2.0×10-10m2/s程度であると予想される場合には、rが、0.62μm程度だとすると、Vdifは、1.0×10-3m/sとなるので、光検出領域の位置の移動速度は、その10倍以上の15mm/sと設定されてよい。なお、発光粒子の拡散係数が未知の場合には、光検出領域の位置の移動速度を種々設定して光強度の変化のプロファイルが、予想されるプロファイル(典型的には、励起光強度分布と略同様)となる条件を見つけるための予備実験を繰り返し実行して、好適な光検出領域の位置の移動速度が決定されてよい。
2πR=v・tcycle …(10)
により与えられるので、式(6)から、光検出領域の移動速度vは、
v>(3πR/r2)・D …(11)
も成立するよう設定される。
上記の処理により試料溶液中の発光粒子の時系列の光強度データが得られると、コンピュータ18に於いて、記憶装置に記憶されたプログラムに従った処理により、光強度データ上に於ける発光粒子からの光に対応する信号の検出、同一の発光粒子の信号の抽出、拡散係数の算出が実行される。
時系列の光強度データに於いて、一つの発光粒子の光検出領域を通過する際の軌跡が、図5(B)に示されている如く略直線状である場合、その粒子に対応する信号に於ける光強度の変化は、(光学系により決定される)光検出領域内の光強度分布を反映した略釣鐘状のプロファイルを有する(図8(B)参照)。従って、走査分子計数法では、基本的には、適宜設定される閾値を超える光強度が継続する時間幅が所定の範囲にあるとき、その光強度のプロファイルを有する信号が一つの粒子が光検出領域を通過したことに対応すると判定され、一つの発光粒子の検出が為されるようになっていてよい。そして、閾値を超える光強度が継続する時間幅が所定の範囲にない信号は、ノイズ又は異物の信号として判定される。また、光検出領域の光強度分布が、ガウス分布:
I=A・exp(-2t2/a2) …(12)
であると仮定できるときには、有意な光強度のプロファイル(バックグラウンドでないと明らかに判断できるプロファイル)に対して式(12)をフィッティングして算出された強度A及び幅aが所定の範囲内にあるとき、その光強度のプロファイルが一つの粒子が光検出領域を通過したことに対応すると判定され、一つの発光粒子の検出が為されてよい。(強度A及び幅aが所定の範囲外にある信号は、ノイズ又は異物の信号として判定され、その後の分析等に於いて無視されてよい。)
時系列光強度データ上に於ける発光粒子のパルス信号の検出が為されると、それらのパルス信号のうちから、同一の発光粒子の信号の抽出が為される。上記までの説明から理解される如く、同一の発光粒子の信号は、光検出領域の移動周期に(完全に一致しないが)概ね等しい周期にて連続的に出現する。そこで、同一の発光粒子の信号の抽出は、光検出領域の移動周期に概ね等しい周期にて連続的に出現する信号を任意の手法又はアルゴリズムにより選択することにより為されてよい。例えば、最も単純には、実験者が時系列光強度データ上に於いて光検出領域の移動周期に概ね等しい周期にて連続的に出現する信号を目視によって特定することにより、同一の発光粒子の信号が抽出されてよい。
ΔT=d/v …(2)
の範囲内に発生した信号は、前記の一つの発光粒子と同一の発光粒子の信号であると判断できる。そこで、同一の発光粒子の信号の抽出方法の一つとして、時系列光強度データ上に於いて、1つの発光粒子の信号の発生時間に光検出領域の周期を加算した時間を中心とする時間幅ΔTの範囲内に発生した信号が、前記の1つの信号に対応する発光粒子と同一の発光粒子の信号であると判定するアルゴリズムが採用されてよい。より具体的には、図7(A)に模式的に例示されている如く、例えば、或る発光粒子の信号α(i)を選択した後、その選択された信号α(i)の発生時刻(ピーク時であってよい。)から光検出領域の一周期が経過する時間を中心として、時間幅ΔTの範囲内に信号α(ii)が存在する場合には、その信号α(ii)が信号α(i)と同一の発光粒子の信号として選択される。しかる後、更に、選択された信号α(ii)の発生時刻から光検出領域の一周期が経過する時間(tcycle)を中心として、時間幅ΔTの範囲内に信号α(iii)が存在する場合には、その信号α(iii)が既に選択された信号α(i)、α(ii)と同一の発光粒子の信号として選択される。かくして、新たに信号が選択される毎に、その選択された信号の発生時刻から光検出領域の一周期が経過する時間を中心として時間幅ΔTの範囲内に存在する信号を選択する処理を順に反復して実行することにより、時系列光強度データ上に於いて、同一の発光粒子の一連の信号(信号群)を抽出することが可能となる。(なお、最初に選択された信号の発生時刻を基準として、光検出領域の移動周期(tcycle)が経過する毎に、各周期が終了する時間を中心として、時間幅ΔTの範囲内に存在する信号が最初に選択された信号と同一の発光粒子の信号として選択されるようになっていてもよい。)
上記の如く(発光粒子毎に)同一の発光粒子の信号群が抽出されると、各信号の発生時間の間隔に於ける光検出領域の移動周期からのずれ時間に基づいて発光粒子の変位が見積もられる。既に触れたように、光検出領域が所定の経路をk周する時間(k・tcycle)の発光粒子の変位は、k回の光検出領域の周回に於ける最初の信号の発生時間toと最後の信号の発生時間tkとの間に於けるk周期に相当する時間k・tcycleからのずれ時間
Δt=tk-to-k・tcycle …(13)
を用いて、式(4)にて算出される。従って、発光粒子の変位の算出に於いては、典型的には、抽出された信号群に於ける信号のうちから二つの信号の組合せの全てについて、式(13)により、ずれ時間Δtが算出され、算出されたΔtに光検出領域の移動速度vが乗ぜられて(式(4)により)、各信号の発生時間の間隔に於ける発光粒子の変位xが算出されてよい。例えば、図7(B)の如く、5個の信号が略周期的な信号が検出されているとすると、光検出領域の移動の1周期、2周期、3周期及び4周期に相当する時間に於けるずれ時間(粒子の変位)を与える信号の組合せ数は、それぞれ、4組(1、5、8、10)、3組(2、6、9)、2組(3、7)及び1組(4)となり、全部で、10個のずれ時間及び粒子の変位のデータが算出されることとなる。そして、発光粒子の信号群の信号数がn個であるときには、1~n-1周期に相当する時間の各々に対して、ずれ時間及び粒子の変位のデータが得られ、データの総数は、n(n-1)/2個となる。
かくして、上記のステップ180に於ける処理により、一つの発光粒子の、光検出領域の移動時間(周回数×移動周期)の各々に於ける粒子の変位が算出されると、式(5)の関係を用いて、その発光粒子の拡散係数Dが算出される。例えば、一つの態様に於いては、図7(C)に模式的に例示されている如く、時間(周回数×移動周期)に対するステップ180で得られた粒子の変位の二乗値x2のプロットに対して、最小二乗法等により直線をフィッティングし、その直線の傾き(=2D)から、拡散係数Dが算出されてよい(直線のフィッティングに於いては、粒子の変位の二乗値x2(図中、×)に対して直線をフィッティングが為されるか、周期毎の粒子の変位の二乗値x2の平均値(図中、●)に対して直線をフィッティングが為されてよい。)。また、それぞれの粒子の変位xについて、
D=x2/(2k・tcycle) …(14)
を算出し、算出されたDの平均値をその発光粒子の拡散係数としてもよい。
本発明の方法により発光粒子の拡散係数が測定されることを検証した。
20μ秒<パルス幅<400μ秒
ピーク強度>1.0[pc/10μs] …(A)
相関係数>0.95
を満たすパルス信号のみを発光粒子に対応する信号であると判定する一方、上記の条件を満たさないパルス信号はノイズとして無視した。
信号1 1510.741
信号2 1520.726
信号3 1530.738
信号4 1540.767
信号5 1550.825
これらの一連の信号の周期は、光検出領域の移動周期に略等しく、同一の発光粒子の信号であると考えられる。そこで、上記の信号のピーク時刻を用いて、「(iii)発光粒子の変位の算出」に記載されている要領にて、5つの信号のうちの二つの信号の組合せの全てについて、式(13)により、信号の発生時間の間隔に於ける移動周期に相当する時間(周期数×移動周期)からのずれ時間Δtを算出し、更に、式(4)により発光粒子の変位xを算出した。図8(C)は、かくして算出された変位xの二乗値(x2)を時間(周期数×移動周期)に対してプロットした図である(各点は、各時間に於ける変位xの二乗値の平均値である。)。図から理解される如く、変位xの二乗値x2の平均値は、時間に対して略比例した。このことは、変位xの二乗値x2の平均値と時間との間に式(5)にて表される関係が成立しており、上記の信号の発生時間の間隔に於ける移動周期に相当する時間からのずれ時間Δtに基づいて、ブラウン運動による発光粒子の変位xが算定できることを示している。なお、図8(C)のプロットに対して、最小二乗法により直線をフィッティングして得られた直線の傾き(=2D)から得られた拡散係数Dは、3.89×10-11[m2/s]であった。
Claims (7)
- 共焦点顕微鏡又は多光子顕微鏡の光学系を用いて試料溶液中にて分散しランダムに運動する発光粒子の拡散特性値を測定する方法であって、
前記光学系の光路を変更することにより前記試料溶液内に於いて前記光学系の光検出領域の位置を所定の経路に沿って周期的に移動する過程と、
前記試料溶液内に於いて前記光検出領域の位置を移動させながら前記光検出領域からの光の強度を測定して光強度データを生成する過程と、
前記光強度データ上に於いて発光粒子の光を表す信号を個別に検出する過程と、
前記検出された発光粒子の光を表す信号のうち、同一の発光粒子に対応する複数の信号を抽出する過程と、
前記抽出された信号の発生時間の間隔に於ける前記光検出領域の移動周期からのずれ時間に基づいて前記抽出された信号に対応する発光粒子の拡散特性値を算出する過程と
を含むことを特徴とする方法。 - 請求項1の方法であって、前記拡散特性値を算出する過程に於いて、前記抽出された信号の発生時間の間隔に於ける前記光検出領域の移動周期からのずれ時間と前記光検出領域の移動速度とから算出される前記抽出された信号に対応する発光粒子の変位に基づいて前記拡散特性値が算出されることを特徴とする方法。
- 請求項1の方法であって、前記抽出された信号の発生時間の間隔が複数の連続した前記抽出された信号のうちのいずれか二つの信号の発生時間の時間差であることを特徴する方法。
- 請求項1の方法であって、前記同一の発光粒子に対応する複数の信号を抽出する過程に於いて、前記検出された発光粒子の光を表す信号のうちの1つの信号の発生時間に前記光検出領域の周期を加算した時間を中心とする前記光検出領域の寸法と移動速度とに基づいて決定される時間幅内に発生した信号が前記1つの信号に対応する発光粒子と同一の発光粒子の信号であると判定されることを特徴とする方法。
- 請求項1の方法であって、前記光強度データ上に於いて複数の発光粒子からの光を表す信号が存在している場合には、前記複数の発光粒子の各々について別々に前記拡散特性値が算出されることを特徴とする方法。
- 請求項1乃至5のいずれかの方法であって、前記拡散特性値が拡散係数であることを特徴とする方法。
- 請求項1乃至6のいずれかの方法であって、前記光検出領域の位置が前記試料溶液中の発光粒子の拡散移動速度よりも速い速度にて移動されることを特徴とする方法。
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Also Published As
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CN103154708B (zh) | 2015-01-14 |
JPWO2012050011A1 (ja) | 2014-02-24 |
US20130314705A1 (en) | 2013-11-28 |
EP2615445A1 (en) | 2013-07-17 |
EP2615445B1 (en) | 2014-05-21 |
JP5904947B2 (ja) | 2016-04-20 |
EP2615445A4 (en) | 2013-07-24 |
CN103154708A (zh) | 2013-06-12 |
US8681332B2 (en) | 2014-03-25 |
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