Classifications

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  • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
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  • C04B35/00—Shaped ceramic products characterised by their composition; Ceramics compositions; Processing powders of inorganic compounds preparatory to the manufacturing of ceramic products
  • C04B35/622—Forming processes; Processing powders of inorganic compounds preparatory to the manufacturing of ceramic products
  • C04B35/626—Preparing or treating the powders individually or as batches ; preparing or treating macroscopic reinforcing agents for ceramic products, e.g. fibres; mechanical aspects section B
  • C04B35/63—Preparing or treating the powders individually or as batches ; preparing or treating macroscopic reinforcing agents for ceramic products, e.g. fibres; mechanical aspects section B using additives specially adapted for forming the products, e.g.. binder binders
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  • C07C311/30—Sulfonamides, the carbon skeleton of the acid part being further substituted by singly-bound nitrogen atoms, not being part of nitro or nitroso groups
  • C07C311/45—Sulfonamides, the carbon skeleton of the acid part being further substituted by singly-bound nitrogen atoms, not being part of nitro or nitroso groups at least one of the singly-bound nitrogen atoms being part of any of the groups, X being a hetero atom, Y being any atom, e.g. N-acylaminosulfonamides
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  • C07D209/80—[b, c]- or [b, d]-condensed
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  • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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  • C07D231/14—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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Definitions

• the present invention relates to compounds useful as inhibitors of voltage-gated sodium channels and calcium channels.
• the invention also provides pharmaceutically acceptable compositions comprising the compounds of the invention and methods of using the compositions in the treatment of various disorders.
• Na channels are central to the generation of action potentials in all excitable cells such as neurons and myocytes . They play key roles in excitable tissue including brain, smooth muscles of the gastrointestinal tract, skeletal muscle, the peripheral nervous system, spinal cord and airway. As such they play key roles in a variety of disease states such as epilepsy (See, Moulard, B. and D. Bertrand (2002) "Epilepsy and sodium channel blockers” Expert Opin. Ther. Patents 12(1): 85-91)), pain (See, axman, S. G., S. Dib-Hajj, et al .
• Voltage gated Na channels comprise a gene family consisting of 9 different subtypes (NaVl .1-NaVl .9) . As shown in Table 1, these subtypes show tissue specific localization and functional differences (See, Goldin, A. L. (2001) "Resurgence of sodium channel research” Annu Rev Physiol 63: 871-94). Three members of the gene family (NaVl .8 , 1.9, 1.5) are resistant to block by the well-known Na channel blocker TTX, demonstrating subtype specificity within this gene family. Mutational analysis has identified glutamate 387 as a critical residue for TTX binding (See, Noda, M. , H. Suzuki, et al . (1989) "A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II" FEBS Lett 259(1): 213-6).
• CNS central nervous system
• PNS peripheral nervous sytem
• DRG dorsal root ganglion
• TG Trigeminal ganglion
• NaVs voltage-gated sodium channels
• Antagonists of NaV channels can attenuate these pain signals and are useful for treating a variety of pain conditions, including but not limited to acute, chronic, inflammatory, and neuropathic pain.
• Known NaV antagonists such as TTX, lidocaine (See, Mao, J. and L. L. Chen (2000) "Systemic lidocaine for neuropathic pain relief” Pain 87(1): 7-17.) bupivacaine, phenytoin (See, Jensen, T. S.
• TTX-resistant currents are insensitive to micromolar concentrations of tetrodotoxin, and displays slow activation and inactivation kinetics and a more depolarized activation threshold when compared to other voltage-gated sodium channels.
• TTX-resistant sodium currents are primarily restricted to a subpopulation of sensory neurons likely to be involved in nociception. Specifically, TTX- resistant sodium currents are expressed almost exclusively in neurons that have a small cell-body diameter; and give rise to small-diameter slow-conducting axons and that are responsive to capsaicin.
• a large body of experimental evidence demonstrates that TTX-resistant sodium channels are expressed on C-fibers and are important in the transmission of nociceptive information to the spinal cord.
• NaVl .8 protein is upregulated along uninjured C-fibers adjacent to the nerve injury. Antisense treatment prevents the redistribution of NaVl .8 along the nerve and reverses neuropathic pain. Taken together the gene-knockout and antisense data support a role for NaVl .8 in the detection and transmission of inflammatory and neuropathic pain.
• expression of NaVl .8 and NaVl .9 are greatly reduced whereas expression of the TTX sensitive subunit NaVl .3 is significantly upregulated in animal models of neuropathic pain (See, Dib-Hajj, S. D., J. Fjell, et al . (1999) "Plasticity of sodium channel expression in DRG neurons in the chronic constriction injury model of neuropathic pain.” Pain 83(3): 591-600 and Kim, C.H., Youngsuk, O. , et al .
• NaVl .3 channel proteins are detectable in injured but not uninjured human nerves indicating that NaVl .3 plays important physiological roles under pathophysiological conditions in humans as well.
• inhibitors of NaVl .3 channels, and in particular selective ones might therefore provide efficacious therapeutic agents with less-severe side effects than nonselective Na_ + channel inhibitors in the treatment of painful neuropathies.
• NaVl .3 overexpression may also be associated with increased epipleptic neuronal activity as it is significantly upregulated in hippocampal pyramidal neurons of epileptic humans (See, Whitaker, W.R.J., Faull, M. , et al..(2001) "Changes in the mRNAs encoding voltage-gated sodium channel types II and III in human epileptic hippocampus.” Neurosci . 106(2): 275-285.); inhibitors with some selectivity against Navl.3 could also be particularly attractive anticonvulsants and neuroprotectants .
• NaVl .9 is similar to NaVl .8 as it is selectively localized to small sensory neurons of the dorsal root ganglion and trigeminal ganglion (See, Fang, X., L. Djouhri, et al . (2002) . "The presence and role of the tetrodotoxin-resistant sodium channel Na (v) 1.9 (NaN) in nociceptive primary afferent neurons.” J Neurosci 22(17) : 7425-33.) . It has a slow rate of inactivation and left-shifted voltage dependence for activation (See, Dib-Hajj, S., J. A. Black, et al .
• NaVl .9 a sodium channel with unique properties
• Trends Neurosci 25(5) : 253-9. These two biophysical properties allow NaVl .9 to play a role in establishing the resting membrane potential of nociceptive neurons.
• the resting membrane potential of NaVl .9 expressing cells is in the - 55 to -50mV range compared to -65mV for most other peripheral and central neurons.
• This persistent depolarization is in large part due to the sustained low-level activation of NaVl .9 channels. This depolarization allows the neurons to more easily reach the threshold for firing action potentials in response to nociceptive stimuli.
• Compounds that block the NaVl .9 channel may play an important role in establishing the set point for detection of painful stimuli.
• NaVl .8 and NaVl .7 are termed neuromas and the primary Na channels expressed in them.
• NaVl .8 and NaVl .7 See, Kretschmer, T., L. T. Happel, et al . (2002) "Accumulation of PN1 and PN3 sodium channels in painful human neuroma- evidence from immunocytochemistry" Acta Neurochir (Wien) 144(8): 803-10; discussion 810.).
• NaVl .6 and NaVl .7 are also expressed in dorsal root ganglion neurons and contribute to the small TTX sensitive component seen in these cells.
• Antagonists for NaVl .5 have been developed and used to treat cardiac arrhythmias.
• a gene defect in NaVl .5 that produces a larger noninactivating component to the current has been linked to long QT in man and the orally available local anesthetic mexilitine has been used to treat this condition (See, Wang, D. W. , K. Yazawa, et al . (1997) "Pharmacological targeting of long QT mutant sodium channels.” J Clin Invest 99(7): 1714-20).
• Na channel blockers are currently used or being tested in the clinic to treat epilepsy (See, Moulard, B. and D. Bertrand (2002) “Epilepsy and sodium channel blockers” Expert Opin. Ther.
• Patents 12(1): 85-91. acute (See, Wiffen, P., S. Collins, et al . (2000) "Anticonvulsant drugs for acute and chronic pain” Cochrane Database Syst Rev 3), chronic (See, Wiffen, P., S. Collins, et al . (2000) “Anticonvulsant drugs for acute and chronic pain” Cochrane Database Syst Rev 3, and Guay, D. R. (2001) "Adjunctive agents in the management of chronic pain” Pharmacotherapy 21(9): 1070-81), inflammatory (See, Gold, M. S.
• Narasimhan (1997) “Sodium channels and therapy of central nervous system diseases” Adv Pharmacol 39: 47-98) and as anesthetics (See, Strichartz, G. R. , Z. Zhou, et al . (2002) "Therapeutic concentrations of local anaesthetics unveil the potential role of sodium channels in neuropathic pain.” Novartis Found Symp 241: 189-201).
• Voltage-gated calcium channels are. membrane-spanning, multi-subunit proteins that open in response to membrane depolarization, allowing Ca entry from the extracellular milieu. Calcium channels were initially classified based on the time and volt ge-dependence of channel opening and on the sensitivity to pharmacological block. The categories were low-voltage activated
• the a. ⁇ subunit is the primary determinant of the pharmacological properties and contains the channel pore and voltage sensor (Hockerman, G. H. , et . al . (1997) Annu. Rev. Pharmacol . Toxicol . 37: 361-96; Striessnig, J. (1999) Cell. Physiol. Biochem. 9: 242-69).
• Ten isoforms of the a. ⁇ subunit are known, as indicated in Table I.
• the ⁇ subunit consists of two disulfide linked subunits, ⁇ 2 , which is primarily extracellular and a transmembrane ⁇ subunit .
• the ⁇ subunit is a non-glycosylated cytoplasmic protein that binds to the ⁇ i subunit .
• Four isoforms are known, termed ⁇ ]to ⁇ 4 .
• the ⁇ subunit is a transmembrane protein that has been biochemically isolated as a component of Ca v l and Ca v 2 channels. At least 8 isoforms are known ( ⁇ i to ⁇ 8 ) (Kang, M.G. and K. P. Campbell (2003) J. Biol. Chem. 278: 21315-8).
• voltage-gated calcium channels is based upon the content of the a ⁇ subunit, as indicated in -Table I.
• Each type of ⁇ subunit can associate with a variety of ⁇ , ⁇ 2 ⁇ or ⁇ subunits, so that each Ca v type corresponds to many different combinations of subunits.
• Ca v 2 currents are found almost exclusively in the central and peripheral nervous system and in neuroendocrine cells and constitute the predominant forms of presynaptic voltage-gated calcium current. Presynaptic action potentials cause channel opening and neurotransmitter release is steeply dependent upon the subsequent calcium entry. Thus, Ca v 2 channels play a central role in mediating neurotransmitter release.
• Ca v 2.1 and Ca v 2.2 contain high affinity binding sites for the peptide toxins ⁇ -conotoxin-MVIIC and ⁇ -conotoxin-GVIA, respectively, and these peptides have been used to determine the distribution and function of each channel type.
• Ca v 2.2 is highly expressed at the presynaptic nerve terminals of neurons from the dorsal root ganglion and neurons of lamina I and II of the dorsal horn (Westenbroek, R. E ., , et al . (1998) J. Neurosci. 18: 6319-30; Cizkova, D, et al . (2002) Exp. Brain Res. 147: 456-63). Ca v 2.2 channels are also found in presynaptic terminals between second and third order interneurons in the spinal cord. Both sites of neurotransmission are very important in relaying pain information to the brain.
• Acute pain serves an important protective function in keeping the organism safe from stimuli that may produce tissue damage. Severe thermal, mechanical, or chemical inputs have the potential to cause severe damage to the organism if unheeded.
• Acute pain serves to quickly remove the individual from the damaging environment . Acute pain by its very nature generally is short lasting and intense. Inflammatory pain, on the other hand, may last for much longer periods of time and its intensity is more graded. Inflammation may occur for many reasons including tissue damage, autoimmune response, and pathogen invasion.
• Inflammatory pain is mediated by a variety of agents that are released during inflammation, including substance P, histamines, acid, prostaglandin, bradykinin, CGRP, cytokines, ATP, and other agents (Julius, D. and A. I. Basbaum (2001) Nature 413 (6852): 203-10).
• the third class of pain is neuropathic and involves nerve damage arising from nerve injury or viral infection and results in reorganization of neuronal proteins and circuits yielding a pathologic "sensitized" state that can produce chronic pain lasting for years. This type of pain provides no adaptive benefit and is particularly difficult to treat with existing therapies.
• Pain, particularly neuropathic and. intractable pain is a large unmet medical need. Millions of individuals suffer from severe pain that is not well controlled by current therapeutics.
• the current drugs used to treat pain include NSAIDS, COX-2 inhibitors, opioids, tricyclic antidepressants , and anticonvulsants.
• Neuropathic pain has been particularly difficult to treat as it does not respond well to opioids until high doses are reached.
• Gabapentin is currently the most widely used ⁇ therapeutic for the treatment of neuropathic pain, although it works in only 60% of patients and has modest efficacy. The drug is generally safe, although sedation is an issue at higher doses.
• the toxin has an 85% success rate for the treatment of pain in humans with a greater potency than morphine.
• An orally available antagonist of Ca v 2.2 should have similar efficacy without the need for intrathecal infusion.
• Ca v 2.1 and Ca v 2.3 are also in neurons of nociceptive pathways and antagonists of these channels could be used to treat pain.
• Antagonists of Ca v 2.1 , Ca v 2.2 or Ca v 2.3 should also be useful for treating other pathologies of the central nervous system that apparently involve excessive calcium entry. Cerebral ischaemia and stroke are associated with excessive calcium entry due to depolarization of neurons.
• the Ca v 2.2 antagonist ziconotide is effective in reducing infarct size in a focal ischemia model using laboratory animals, suggesting that Ca v 2.2 antagonists could be used for the treatment of stroke.
• reducing excessive calcium influx into neurons may be useful for the treatment of epilepsy, traumatic brain injury, Alzheimer's disease, multi-infarct dementia and other classes of dementia, amyotrophic lateral sclerosis, amnesia, or neuronal damage caused by poison or other toxic substances.
• Ca v 2.2 also mediates release of neurotransmitters from neurons of the sympathetic nervous system and antagonists could be used to treat cardiovascular diseases such as hypertension, cardiac arrhythmia, angina pectoris, myocardial infarction, and congestive heart failure.
• R5; R 3 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring optionally substituted with up to 3 substituents, independently selected from R 1 , R 2 , R 4 or R 5 ;
• R 4 is OR 5 , OR 6 , OC(0)R 6 , 0C(0)R 5 , OC(0)OR 6 , OC(0)OR 5 , OC(0)N(R 6 ) 2 , 0C(0)N(R 5 ) 2 , OC (0) N (R 6 R 5 ) , OP(0) (OR 6 ) 2 , OP(0) (OR 5 ) 2 , OP (O) (OR 6 ) (OR 5 ) , SR 6 , SR 5 , S(0)R 6 , S(0)R 5 , S0 2 R 6 , S0 2 R 5 .
• R 5 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring optionally substituted with up to 3 R 1 substituents
• R 6 is H or aliphatic, wherein R 6 is optionally substituted with a R 7 substituent
• R 7 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring and each R 7 is optionally substituted with up to 2 substituents independently chosen from H, aliphatic, or (CH 2 .
• n ⁇ Z ' Z' is selected from halo, CN, N0 , C(halo) 3 , CH(halo)2. CH 2 (halo) , -OC(halo) 3 , -OCH(halo) 2 . -OCH 2 (halo) ,OH, S-aliphatic, S (0) -aliphatic, S ⁇ 2 ⁇ aliphatic, NH 2 NH-aliphatic, N (aliphatic) 2.
• N (aliphatic) R 8 COOH, C (0) 0 (-aliphatic) , or O-aliphatic; and R 8 is an amino protecting group.
• These compounds and pharmaceutically acceptable compositions thereof are useful for treating or lessening the severity of a variety of diseases, disorders, or conditions, including, but not limited to, acute, chronic, neuropathic, or inflammatory pain, arthritis, migraine, cluster headaches, trigeminal neuralgia, herpetic neuralgia, general neuralgias, epilepsy or epilepsy conditions, neurodegenerative disorders, psychiatric disorders such as anxiety and depression, myotonia, arrythmia, movement disorders, neuroendocrine disorders, ataxia, multiple sclerosis, irritable bowel syndrome, and incontinence.
• diseases, disorders, or conditions including, but not limited to, acute, chronic, neuropathic, or inflammatory pain, arthritis, migraine, cluster headaches, trigeminal neuralgia, herpetic neuralgia, general neuralgias, epilepsy or epilepsy conditions, neurodegenerative disorders, psychiatric disorders such as anxiety and depression, myotonia, arrythmia, movement disorders
• FIGURE 1 (FIGURE 1-1 to FIGURE 1-122) depicts the structures of the compounds of the present invention.
• the present invention provides compounds of formula (I) useful in inhibiting a sodium and/or calcium channel : T L,—A L 2 —Z (I) . or a pharmaceutically acceptable salt thereof; wherein: Li is -(X ⁇ )p- (X 2 )q-R y -; wherein: X! is 0, S, or NR X p is 0 or 1; q is 0 or 1; R x is H or R 2 ; X 2 is R 2 ; Ry is -C(0)-NR 2 -; or L2 and Ry are independently selected from OC(O), C(0)0, S (O) ,
• N(OR 6 ) Z is hydrogen, cycloaliphatic, heterocyclic, aryl, or heteroaryl ring; T is aliphatic, cycloaliphatic, aryl, heteroaryl, or heterocyclic ring; A is aryl or heteroaryl ring; wherein each of T, A, and Z is optionally substituted with up to 4 suitable substituents independently selected from R 1 , R 2 , R 3 ,
• R 4 or R 5 ;
• Y is halo, CN, N0 2 , CF 3 , 0CF 3 , OH, SR 6 , S(0)R 6 , S0 2 R 6 , NH 2 ,
• R 2 is aliphatic, wherein each R 2 is optionally substituted with up to 2 substituents independently selected from R 1 , R 4 , or
• R 3 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring is optionally substituted with up to 3 substituents, independently selected from R 1 , R 2 , R 4 or R 5 ;
• R 4 is OR 5 , OR 6 , OC(0)R 6 , 0C(0)R 5 , OC(0)OR 6 , OC(0)OR 5 , OC(0)N(R 6 ) 2 , OC(0)N(R 5 ) 2 , OC (0) N (R 6 R 5 ) , OP(0) (OR 6 ) 2 , OP(0) (OR 5 ) 2 , OP(0) (OR 6 ) (OR 5 ) , OP (0) (OR 6 ) (OR 5 ) , SR 6 , SR 5 , S(0)R 6 , S(0)R 5 , S0 2 R 6 .
• N (aliphatic) R 8 COOH, C (O) O (-aliphatic) , or O-aliphatic; and R 8 is an amino protecting group.
• the present invention provides compounds of formula I ' :
• I' or a pharmaceutically acceptable salt thereof, wherein: l is 0, S, or NR X . p is 0 or 1; q is 0 or 1; R x is H or R 2 ; ⁇ 2 is a bond R 2 ; L 2 is selected from OC (0) , C(0)0, S (0) , S0 2 , N(R 5 )S0 2 ,
• Z is cycloaliphatic, heterocyclic, aryl, or heteroaryl ring
• T is aliphatic, cycloaliphatic, aryl, heteroaryl, or heterocyclic ring
• A is aryl or heteroaryl ring; wherein each of T, A, and Z is optionally substituted with up to 4 suitable substituents independently selected from R 1 , R 2 , R 3 ,
• R 4 or R 5 ;
• Y is halo, CN, N0 2 , CF 3 , 0CF 3 , OH, SR 6 , S(0)R 6 , S0 2 R 6 , NH 2 ,
• R 2 is aliphatic, wherein each R 2 is optionally substituted with up to 2 substituents independently selected from R 1 , R 4 , or
• R 5 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring, optionally substituted with up to 3 substituents, independently selected from R 1 , R 2 , R 4 or R 5 ;
• R 4 is OR 5 , OR 6 , 0C(0)R 6 , OC(0)R 5 , OC(0)OR 6 , OC(0)OR 5 , OC(0)N(R 6 ) 2 , OC(0)N(R 5 ) 2 , OC (O) N (R 6 R 5 ) , OP(0) (OR 6 ) 2 , OP(0) (OR 5 ) 2 , OP(0) (OR 6 ) (OR 5 ) , SR 6 , SR 5 , S(0)R 6 , S(0)R 5 , S0 2 R 6 , S0 2 R 5 , S0 2 N(R 6 ) 2 , S0 2 N(R 5 ) 2 , S0 2 NR 5 R 6 , SO3R 6 , SO
• N (aliphatic) R 8 COOH, C (0) O (-aliphatic) , or O-aliphatic; and R 8 is an amino protecting group.
• compounds of the invention may optionally be substituted with one or more substituents, such as are illustrated generally above, or as exemplified by particular classes, subclasses, and species of the invention. It will be appreciated that the phrase “optionally substituted” is used interchangeably with the phrase “substituted or unsubstituted.” In general, the term “substituted”, whether preceded by the term “optionally” or not, refers to the replacement of hydrogen radicals in a given structure with the radical of a specified substituent.
• an optionally substituted group may have a substituent at each substitutable (i.e., having the requisite valency available for a given substituent) position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position.
• Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds.
• the term "stable”, as used herein, refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, and preferably their recovery, purification, and use for one or more of the purposes disclosed herein.
• a stable compound or chemically feasible compound is one that is not substantially altered when kept at a temperature of 40°C or less, in the absence of moisture or other chemically reactive conditions, for at least a week.
• aliphatic or "aliphatic group”, as used herein, means a straight-chain (i.e., unbranched) or branched, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation. Unless otherwise specified, aliphatic groups contain 1-20 aliphatic carbon atoms. In some embodiments, aliphatic groups contain 1-10 aliphatic carbon atoms. In other embodiments, aliphatic groups contain 1-8 aliphatic carbon atoms. In still other embodiments, aliphatic groups contain 1-6 aliphatic carbon atoms, and in yet other embodiments aliphatic groups contain 1-4 aliphatic carbon atoms.
• cycloaliphatic refers to a monocyclic C 3 -C 8 hydrocarbon or bicyclic C 8 -C 12 hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule wherein any individual ring in said bicyclic ring system has 3-7 members.
• heterocycle means non-aromatic, monocyclic, bicyclic, or tricyclic ring systems in which one or more ring atoms in one or more ring members is an independently selected heteroatom.
• heteroatom means oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3 , 4-dihydro-2H-pyrrolyl) , NH (as in pyrrolidinyl) or NR + (as in N-substituted pyrrolidinyl) ) .
• unsaturated means that a moiety has one or more units of unsaturation.
• aryl used alone or as part of a larger moiety as in “aralkyl”, “aralkoxy”, or “aryloxyalkyl” , refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring carbon atoms, wherein at least one ring in the system is aromatic and wherein each ring in the system contains 3 to 7 ring carbon atoms.
• aryl may be used interchangeably with the term “aryl ring” .
• heteroaryl used alone or as part of a larger moiety as in “heteroaralkyl” or “heteroarylalkoxy”, refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic, at least one ring in the system contains one or more heteroatoms, and wherein each ring in the system contains 3 to 7 ring members.
• heteroaryl may be used interchangeably with the term “heteroaryl ring” or the term
• alkylidene chain refers to a straight or branched carbon chain that may be fully saturated or have one or more units of unsaturation and has two points of attachment to the rest of the molecule.
• structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers. Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, and geometric (or conformational) mixtures of the present compounds are within the scope of the invention. Unless otherwise stated, all tautomeric forms of the compounds of the invention are within the scope of the invention.
• structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms.
• compounds having the present structures except for the replacement of hydrogen by deuterium or tritium, or the replacement of a carbon by a 13 C- or 14 C-enriched carbon are within the scope of this invention.
• Such compounds are useful, for example, as analytical tools or probes in biological assays.
• p is 0, and q is 0.
• p is 0, and q is 1.
• p is 1, and q is 0.
• p is 1 and q is 1.
• X 2 is a straight or branched (C1-C6) alkyl or (C2-C6) alkenyl or alkynyl, optionally substituted with up to two substituents independently selected from Ri and R5. More preferably, X 2 is a straight or branched (Cl-
• alkyl optionally substituted with up to two substituents independently selected from R ⁇ and R5.
• Preferred X 2 include Cl-4 alkyl, such as, -CH 2 -, CH 2 CH 2 , or -CH 2 CH 2 CH 2 - .
• R 2 is straight or branched (C1-C6) alkyl or (C2-C6) alkenyl or alkynyl, optionally substituted with up to two substituents independently selected from R]_ and R 5 . More preferably, Ry is -C (O) -NH- .
• L 2 is selected from N(R 5 )S0 2 , N(R 6 )S0 2 ,
• L 2 is selected from N (R 6 ) S0 2 ,
• R s is hydrogen
• L 2 is selected from NHS0 2 , SO2NH,
• Z is cycloaliphatic, heterocyclic, aryl, or heteroaryl ring.
• Z is aryl or heteroaryl. More preferably, Z is phenyl or napthyl .
• Z is heteroaryl. More preferably, Z is selected from thiazole, isothiazole, thiadiazole, thiaphene, furan, oxazole, isooxazole, oxadiazole, triazole, imidazole, pyrazole, pyridine, pyrimidine, pyrazine, pyridazine, triazine, or pyrrolyl .
• A is aryl. More preferably, A is phenyl or naphthyl. Most preferably,
• A is phenyl .
• A is heteroaryl. More preferably, A is a monocyclic aromatic ring containing 1 to 3 heteroatoms. More preferably, A is pyridyl, pyrazyl, triazinyl, furanyl , pyrrolyl, thiophenyl, oxazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, imidazolyl, triazolyl, thiadiazolyl , or pyrimidinyl . According to another preferred embodiment of formula (I) , A is a bicyclic ring system with at least one aromatic ring, wherein said ring system contains 1-5 heteroatoms.
• A is quinolinyl, isoquinolinyl, benzofuranyl, benzothiophenyl, quinolinyl, isoquinolinyl, benzofuranyl, benzothiophenyl, indolizinyl, indolyl, isoindolyl, indolinyl, indazolyl, benzimidazolyl, benzothiazolyl , purinyl, cinnolinyl, phthalazine, quinazolinyl , quinaoxalinyl, naphthylirinyl, or pteridinyl .
• A is a tricyclic ring system with at least one aromatic ring, wherein said ring system contains 1-5 heteroatoms. More preferably, A is dibenzofuranyl , carbazolyl, acridinyl, phenazinyl, phenothiazinyl, or phenoxazinyl .
• T is aliphatic or cycloaliphatic.
• T is aliphatic; more preferably, (C1-C6) straight or branched alkyl; yet more preferably, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, or t-butyl.
• T is cycloaliphatic; more preferably, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, norbornyl, or adamantyl.
• T is cyclopropyl, cyclohenxyl, norbornyl, or adamantyl .
• T is an aryl ring; more preferably, phenyl, napthyl , or anthracenyl . Yet more preferably, T is phenyl or napthyl. According to another preferred embodiment, T is a heteroaryl ring; more preferably, thiophenyl, benzothiophenyl, pyridyl, furanyl, benzofuranyl, oxazolyl, quinolinyl, thiophenyl, benzothiophenyl, pyridiyl, furanyl, benzofuranyl, oxazolyl, quinolinyl, pyrrolyl, thiazolyl, imidazolyl, pyrazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, triazolyl, thiadiazolyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazin
• T is optionally substituted with up to three substituents independently selected from phenyl optionally substituted with R 1 , halo, cyano, trifluoromethyl, OH, C ⁇ _ 4 alkyl, C 2 - 4 alkenyl, C x - 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C ⁇ _ 4 alkyl), N(C x - 4 alkyl) 2 , NHC(0)d- 4 alkyl, or C(0)C!-4 alkyl.
• T is a heterocyclic ring; preferably, tetrahydrofuranyl , pyrrolidinyl, piperidinyl, morpholinyl, dithianyl, thiomorpholinyl, piperazinyl, quinuclidinyl, tetrahydrofuranyl , pyrrolidinyl, piperidinyl, morpholinyl, dithianyl, thiomorpholinyl, piperazinyl, quinuclidinyl, dioxoianyl, imidazolidinyl , pyrazolidinyl, dioxanyl , piperazinyl, or trithianyl .
• R 1 is oxo.
• R 1 is R 6 or (CH 2 ) ⁇ _-Y; more preferably, R 1 is Y (i.e., n is 0).
• R 2 is a straight or branched (C1-C6) alkyl or (C2-C6) alkenyl or alkynyl, optionally substituted with up to two R 1 substitutions.
• R 1 is (CH ) n -Y.
• R 1 is Y.
• Preferred Y includes halo, CN, N0 2 , CF3 , OCF 3 , OH, SH, S(Cl-4 aliphatic), S(0) (Cl-4 aliphatic), S0 2 (Cl-4 aliphatic), NH 2 , NH(C1-
• R 1 is selected from halo, cyano, trifluoromethyl, OH, C ⁇ - 4 alkyl, C 2 - 4 alkenyl, C 1 - 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C ⁇ _ 4 alkyl), N(C ⁇ -. 4 alkyl) 2 , NHC(0)C ⁇ - 4 alkyl, 1-pyrrolidinyl , 1-piperidinyl, 1- morpholinyl, or C (O) C ⁇ _ 4 alkyl.
• Z is thiazol-2-yl ;
• A is phenyl, pyridyl, pyrazinyl, pyridazinyl, pyrimidinyl, triazinyl, or tetrazinyl;
• L X is -(X ⁇ )p-(X 2 )q-Ry-; wherein: Xl is O, S, or NR X p is 0 or 1; q is 0 or 1 ; R x is H or R 2 ; X 2 is R 2 ; R y is -C(0)-NH-; and L 2 is S0 2 N(R 5 ) or S0 2 N(R 6 ).
• the present invention provides a compound of formula I-A:
• X x is 0, S, or NR X p is 0 or 1;
• R x is H or R 2 ;
• R N is hydrogen or Cl-4 aliphatic optionally substituted with up to two substituents selected from R 1 , R 4 , or R 5 ;
• X 2 is C ⁇ _ 3 aliphatic, optionally substituted with up to 2 substituents independently selected from R 1 , R 4 , or R 5 ;
• Z is a 5-7 membered unsaturated or aromatic ring having 1-4 heteroatoms selected from 0, S, SO, S0 2 , N, or NH;
• T is a 8-14 membered aromatic or non-aromatic bicyclic or tricyclic ring, having 0-5 heteroatoms selected from O, S, N, NH, S (O) or S0 2 ; wherein each of Z and T is optionally substituted with up to
• R 5 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring is optionally substituted with up to 3 substituents, independently selected from R 1 , R 2 , R 4 or R 5 ;
• R 4 is OR 5 , OR 6 , OC(0)R 6 , OC(0)R 5 , OC(0)OR 6 , OC(0)OR 5 ,
• NR 5 S0 2 NR 5 R 6 NR 5 S0 2 (R 5 ) 2 .
• N(0R 6 )R 6 N(0R 6 )R 5 ,
• R 5 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring optionally is optionally substituted with up to 3 R 1 substituents
• R 6 is H or aliphatic, wherein R 6 is optionally substituted with a R 7 substituent
• R 7 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring and each R 7 is optionally substituted with up to 2 substituents independently chosen from H, aliphatic, or (CH ) n -z ⁇ Z' is selected from halo, CN, N0 2 , C (halo) 3 , CH(halo) 2 ,
• compounds of formula I or formula I-A exclude the following: a) when both R N are hydrogen, and T is isoindol-1, 3-dione-2-yl optionally substituted with up to 4 halo atoms, then Z is not pyridyl, thiazol-2-yl , 4- (4-methoxyphenyl) thiazol-2-yl , 2-ethyl- 1,3 ,4-thiadiazol-5-yl, optionally substituted pyrimidin-2-yl, 5- methyl-isoxazolyl, 3 , 4-dimethyl-isoxazoly, or 2-methyl-isoxazolyl ;
• R N when both R N are hydrogen, and T is , optionally substituted with up to 4 halo atoms, wherein R mm is phenyl optionally substituted with C X - 4 alkyl or hydrogen, then Z is not optionally substituted pyrimidin-2-yl, 2-pyridyl, or thiazol-2-yl ; c) when both R N are hydrogen, X 2 is _-CH 2 -, p is 1, Xi is S,
• z is not thiazol- 2 -yl , 2, 6-dimethyl-pyrimidin-4-yl, or 3 , 4-dimethyl-isoxazol-5-yl ; e) when both R N are hydrogen, X 2 is -CH 2 -, Xi is 0 or S, and T
• R 1111 is hydrogen or halo, then Z is not thiazol-2-yl , 4-methyl-pyrimidin-2-yl , 4 , 6-dimethylpyri idin-2-yl , pyrimidin-2-yl, or 5-methyl-isoxazol-3-yl ;
• each structure fragment denotes the carbon atom attached to the remainder of the molecule; e.g., the fragment — * denotes an ethyl group, wherein the second atom of that ethyl group is attached to the remainder of the molecule.
• T is attached to X x or to X 2 (when Xx is absent) through a carbon ring atom in T.
• Z is an optionally substituted ring selected from:
• Z is selected from:
• Z has up to two substituents selected from R 1 , R 2 , or R 5 .
• Z is selected from: i-a i-b or i-c.
• Z is formula i-a.
• Z is selected from:
• Z is selected from:
• Z is selected from:
• Z is selected from:
• Z is selected from: vi-a vii-a or vii-b .
• Z is selected from:
• Z is selected from:
• Z is selected from :
• Z is selected from: ix-a ix-b or ix-c .
• R N is hydrogen. Or, R N is unsubstituted Cl-4 alkyl.
• p is 1 and X x is 0.
• p is 1, and X x is S.
• p is 1, and x is NR N .
• R N is hydrogen
• T is naphthyl, tetralinyl, decalinyl, or 6,7,8,9- tetrahydro-5H-benzo [7] annulenyl, optionally substituted with up to 3 substituents independently selected from halo, cyano, trifluoromethyl, OH, C ⁇ - 4 alkyl, C 2 - 4 alkenyl, C ⁇ - 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C ⁇ _ 4 alkyl), N(C ⁇ _ 4 alkyl) 2 , NHC(0)C ⁇ - 4 alkyl, 1-pyrrolidinyl, 1-piperidinyl , 1- morpholinyl, or C(0)C ⁇ - 4 alkyl.
• substituents independently selected from halo, cyano, trifluoromethyl, OH, C ⁇ - 4 alkyl, C 2 - 4 alkenyl, C ⁇ - 4 alkoxy, trifluoromethoxy, C(0)NH 2
• T is optionally substituted napthyl.
• T is selected from:
• T is optionally substituted with up to three substituents independently selected from halo, cyano, trifluoromethyl, OH, C 1-4 alkyl, C 2 - 4 alkenyl, C ⁇ - 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C_.- 4 alkyl), N(d- alkyl) 2 , NHC(0)C 1 . 4 alkyl, or C(0)C 1 . 4 alkyl.
• T is a_ 5-membered ring having up to 4 heteroatoms selected from 0, S, N, or NH, optinally fused to a phenyl ring, wherein said phenyl ring is unsubstituted or substituted with up to 4 substituents selected from R 1 or R 2 .
• Preferred 5-membered rings in such embodiments of T include formula i through xxiii defined above for ring Z that are capable of being fused to a phenyl ring .
• T is selected from:
• T is optionally substituted with up to three substituents independently selected from halo, cyano, trifluoromethyl, OH, C ⁇ - 4 alkyl, C 2 - 4 alkenyl, C ⁇ - 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C ⁇ . 4 alkyl), N (C ⁇ , 4 alkyl) 2 , NHC(0)C 1-4 alkyl, or C(0)C_- 4 alkyl.
• substituents independently selected from halo, cyano, trifluoromethyl, OH, C ⁇ - 4 alkyl, C 2 - 4 alkenyl, C ⁇ - 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C ⁇ . 4 alkyl), N (C ⁇ , 4 alkyl) 2 , NHC(0)C 1-4 alkyl, or C(0)C_- 4 alkyl.
• the phenylene ring attached to the sulfonyl group is optionally substituted with up two substituents selected from halo, cyano, trifluoromethyl , OH, C ⁇ - 4 alkyl, C 2 _ 4 alkenyl, C ⁇ - 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(Ci- 4 alkyl), N(Ci- 4 alkyl) 2 , NHC (0) C_.- alkyl, or C (O) C ⁇ - 4 alkyl .
• the present invention provides compounds wherein: a. Z is thiazol-2-yl; b.
• R N is hydrogen; c. X 2 is absent or is Cl-4 alkylene optionally substituted with phenyl; d. X x is absent or is O or S; e. T is selected from quinolin-4-yl, benzofuran-2-yl, benzothiophen-3-yl, phenyl, tetralin-2-yl, tetralin-6- yl, phenyl, indol-2-yl, chroman-3-yl , quinolin-3-yl , benzo [1,3] oxathiol-2-one-6-yl , benzothiophen-2-yl, 1,2, 3,4-tetrazol-5-yl, furan-5-yl, quinolin-5-yl, benzothiazol-5-yl, or 5, 6 , 7 , 8-tetrahydroquinolin-2-yl, optionally substituted with up to three substituents selected from trifluoromethyl, halo,
• the present invention provides compounds wherein: a. Z is thiazol-2-yl; b. R N is hydrogen; c. X 2 is absent or is Cl-4 alkylene optionally substituted with phenyl; d. i is absent or is O or S; e.
• T is selected from 8-trifluoromethyl-quinolin-4-yl , benzofuran-2-yl, benzothiophen-3-yl , 3-fluoro-4- chloro-phenyl, 8-methoxy-tetralin-2-yl, tetralin-6-yl, 4-piperidinylsulfonylphenyl, 2 , 4-dichlorophenyl, 5- fluoroindol-2-yl, 4 , 6-dichloroindol-2-yl , chroman-3- yl, 2-methyl-6-fluoro-quinolin-4-yl , 2 , 7-dimethyl- quinolin-3-yl, 4-trifluoromethylphenyl, 2-fluoro-4- chloro-phenyl, benzo [1, 3] oxathiol-2-one-6-yl, 5- chloro-benzothiophen-2-yl, 1-phenyl-l, 2,3, 4-tetrazol-
• the present invention provides compounds wherein: a. Z is thiazol-2-yl or 1, 2 , 4-thiadiazol-5-yl ; b. R N is hydrogen; c. X 2 is absent or Cl-4 alkylene; d. Xi is absent or O; e.
• T is selected from phenyl, benzo [1, 3] oxathiol-2- one-5-yl, benzothiophen-2-yl, benzofuran-2-yl , quinolin-4-yl, indolin-2-yl, 1, 2 , 3 , 4-tetrazol-5-yl, 5,6,7, 8-tetrahydroquinolin-2-yl, indol-2-yl, norbornyl, furan-2-yl, 2-naphthyl, benzothiophen-3-yl, phenyl, quinolin-7-yl, tetralin-6-yl , benzothiophen-3- yl, tetralin-2-yl, chroman-3-yl, benzo [1,2,5] oxadiazol-5-yl, quinolin-5-yl , benzothiazol-5-yl, indol-5-yl, quinolin-3-yl, 1,2,3,4-
• the present invention provides compounds wherein: a. Z is thiazol-2-yl or 1, 2 , 4-thiadiazol-5-yl ; b. R N is hydrogen; c. X 2 is absent or Cl-4 alkylene; d. X x is absent or 0; e.
• T is selected from 4-trifluoromethylphenyl, 3- fluoro-4-chlorophenyl, 2-chloro-4-cyanophenyl, 2,3- dichlorophenyl , benzo [1, 3] oxathiol-2-one-5-yl, 5- fluorobenzothiophen-2-yl, 3, 4-dichlorophenyl , benzofuran-2-yl, 8 -trifluoromethyl-quinolin-4-yl, 2- chloro-4-cyanophenyl, l-acyl-indolin-2-yl, 1-phenyl- 1, 2, 3 , 4-tetrazol-5-yl, 2-fluoro-3-chlorophenyl , 2- methyl-4-fluorophenyl, 2, 3-difluorophenyl, 3 -cyano- 5, 6, 7, 8-tetrahydroquinolin-2-yl, 2-chlorophenyl, 5- fluoro-indol-2-yl, 2 , 4-d
• the present invention provides compounds wherein: a. Z is thiazol-2-yl, oxazol-2-yl, 1, 3 , 4-thiadiazol- 2-yl, 1, 2,4-thiadiazol-5-yl, wherein Z is optionally substituted with CF 3 , Cl-4 alkyl, or Cl-4 alkyl substituted with phenyl having 0-3 halo substituents.
• Z is thiazol-2-yl, 5-benzyl-thiazol-2-yl, 5- (4 ' -chlorobenzyl) -oxazol-2-yl , 5-trifluoromethyl- l,3,4-thiadiazol-2-yl, 5- (2 ' -chlorobenzyl) -1,3,4- thiadiazol-2-yl, 5-cyclopropyl-l, 3 ,4-thiadiazol-2-yl, 3-ethyl-l,2,4-thiadiazol-2-yl, or 5-(2',3'- dichlorobenzyl) -thiazol-2-yl; b.
• R N is hydrogen; c.
• X 2 is Cl-3 alkylene; d. Xi is O or is absent; and e. T is phenyl or 3-methyl-l, 2, 3, 4-tetrahydro- isoquinolin-2-yl, wherein T has up to 2 substituents selected from halo, cyano, trifluoromethyl, OH, C..- 4 alkyl, C 2 . 4 alkenyl, C ⁇ - 4 alkoxy, trifluoromethoxy, C(0)NH, NH 2 , NH(Ci- 4 alkyl), N(C ⁇ - 4 alkyl) 2 , NHC (O) C 1- alkyl, or C (O) C ⁇ - 4 alkyl.
• T is 2,4- dichlorophenyl or 3-methyl-l, 2, 3 , 4-tetrahydro- isoquinolin-2-yl .
• the present invention provides compounds wherein: a. Z is selected from thiazol-2-yl, 1, 2 , 4-thiadiazol- 5-yl, 2-pyrazol-3-yl, 1 , 3 , 4-thiadiazol-2-yl , 1,2,5- thiadiazol-4-yl, or 1, 2, 3 ,4-thiatriazol-5-yl, optionally substituted with up to two substituents selected from Cl-4 alkyl, phenyl, or halo.
• Preferred Z includes 3-isopropyl-l, 2 , 4-thiadiazol-5-yl , thiazol- 2-yl, 2, 5-dimethyl-l, 2-pyrazol-3-yl, 5-phenyl-l, 3 , 4- thiadiazol-2-yl, 1, 2 , 5-thiadiazol-4-yl , 5-ethyl-l, 3 , 4- thiadiazol-2-yl, 2-methyl-l, 2-pyrazol-3-yl, 1,2,3,4- thiatriazol-5-yl; b.
• R N is hydrogen;
• X 2 is absent or is Cl-3 alkylene;
• d. i is absent or is O; and e.
• T is selected from quinolinyl, preferably, quinolin-7-yl, dihalo-substituted phenyl, preferably dichlorophenyl, or naphthyl, preferably, 1-naphthyl.
• the present invention provides compounds wherein: a. Z is selected from thiazol-2-yl, 1, 3 , -thiadiazol- 2-yl, pyrimidin-2 -yl, pyrimidin-2 -yl, 1, 2 , 4-triazol-3- yl, or 3-t-butyl-l,2-pyrazol-5-yl, optionally substituted with Cl-4 alkyl, or benzyl; b. R N is hydrogen; c. X 2 is absent or Cl-4 alkylene or alkenylene; d. Xi is absent or O; e.
• T is selected from phenyl, naphthyl, 2,2,- difluoro-benzo [1, 3] dioxol-5-yl, norbornyl, indol-2-yl, benzothiophen-3 -yl, benzo [1,3] oxathiol-2-one-5-yl, benzo [1, 2, 5] oxadiazol-5-yl, quinolinyl, or 1,2,3,4- tetralin-5-yl, optionally substituted with up to 3 substituents selected from halo, cyano, trifluoromethyl, C 1 - 4 alkyl, C 2 -4 alkenyl, C_._ 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH(C ⁇ _ 4 alkyl), N(C_- 4 alkyl) 2 , NHC(0)C 1 . 4 alkyl, C(0)d- 4 alkyl, or 1- piperidyl .
• the present invention provides compounds wherein: a. Z is selected from thiazol-2-yl , 5-methyl-l, 3 ,4- thiadiazol-2-yl, pyrimidin-2 -yl , 4-methyl -pyrimidin-2 - yl, l,2,4-triazol-3-yl, or l-benzyl-3-t-butyl-l, 2- pyrazol-5-yl; b. R N is hydrogen; c. X 2 is absent or is Cl-4 straight or branched alkylene or alkenylene, optionally substituted with phenyl ; d. Xi is absent or is 0; and e.
• T is selected from phenyl, 2 , 2, -difluoro- benzo [1, 3] dioxol-5-yl, norbornyl, indol-2-yl, benzothiophen-3-yl, benzo [1, 3] oxathiol-2-one-5-yl, benzo [1, 2 , 5] oxadiazol-5-yl, quinolinyl, or 1,2,3,4- tetralin-5-yl, optionally substituted with up to 3 substituents selected from halo, cyano, trifluoromethyl, C ⁇ _ alkyl, C 2 - 4 alkenyl, d- 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH(d- 4 alkyl), N(C ⁇ _ 4 alkyl) 2 , NHC (O) d-4 alkyl, or C (O) d- 4 alkyl.
• the present invention provides compounds wherein: a. Z is selected from thiazol-2-yl, 5-methyl-l, 3 , 4- thiadiazol-2-yl , pyrimidin-2 -yl , 4-methyl-pyrimidin-2- yl, or 1, 2 , 4-triazol-3-yl; b. R N is hydrogen; c. X 2 is absent; or X 2 is Cl-4 straight or branched alkyl ; d. Xx is absent; or Xi is O; e.
• T is 2 , 6-dichlorophenyl, 3-diethyaminophenyl , 2- methyl , 4-fluorophenyl , 2-cyanopheny1 , 2-ethoxyphenyl , 2-chlorophenyl, 4-cyanopheny1 , 1-naphthyl, 5- methoxybenzofuran-2-yl, 6-chlorobenzofuran-2-yl, 2- methyl-5, 7-dichloro-quinolin-8-yl, 2-piperidinyl- phenyl, 1, 2 , 3 , 4-tetralin-6-yl, 2-dimethyl-4 , 7- dimethyl-1, 2, 3, 4-tetrahydroquinolin-l-yl, 2,6- difluorophenyl, 3 -fluorophenyl, 2-fluoro-3- chlorophenyl , 2 , 5-dimethylphenyl , 2 , 4-dichlorophenyl , 4-chlorophenyl,
• the present invention provides a compound of formula III:
• Z N is a 5-7 membered monocyclic, unsaturated or aromatic, heterocyclic ring, having up to 4 heteroatoms independently selected from 0, N, NH, S, SO, or S0 2 ; each R N is is independently hydrogen or Cl-4 aliphatic optionally substituted with up to two substituents selected from RI, R , or R5; X2 is d- 3 aliphatic, optionally substituted with up to
• R 6 R 7 NN (R 6 R 7 ) , R 6 or (CH 2 ) n -Y ; n is 0 , 1 or 2 ; Y is halo, CN, N0 2 , CF 3 , OCF 3 , OH, SR 6 , S(0)R 6 , S0 2 R 6 ,
• R 2 is aliphatic, wherein each R 2 is optionally substituted with up to 2 substituents independently selected from R 1 , R 4 , or R 5 ;
• R 3 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring is optionally substituted with up to 3 substituents, independently selected from R 1 , R 2 , R 4 or R 5 ;
• R 4 is OR 5 , OR 6 , OC(0)R 6 , OC(0)R 5 , OC(0)OR 6 , OC(0)OR 5 , 0C(0)N(R 6 ) 2 , OC(0)N(R 5 ) 2 , OC (O) N (R 6 R 5 ) , OP(0) (OR 6 ) 2
• R 5 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring optionally is optionally substituted with up to 3 R 1 substituents;
• R 6 is H or aliphatic, wherein R 6 is optionally substituted with a R 7 substituent;
• R 7 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring and each R 7 is optionally substituted with up to 2 substituents independently chosen from H, aliphatic, or (CH2.
• n -Z ⁇ - Z' is selected from halo, CN, NO2, C (halo) 3 , CH(halo) 2 . CH 2 (halo) , -0C(halo) 3 , -0CH(halo) 2 , - OCH2 (halo) ,0H, S-aliphatic, S (0) -aliphatic, S ⁇ 2-aliphatic,
• T N is not: (i) 1, 3-dione-isoindol-2-yl, 1 , 3-dione-isoindol- 2-yl substituted with up to 4 halo substituents;
• Z N is selected from:
• Z N is selected from: i - a i-b or l-c .
• Z N is formula i-a.
• Z N is selected from:
• Z N is selected from: v-a v-b or v-c ,
• Z N is selected from: ii-a ii-b or iii-a.
• Z N is selected from: vi -a vii-a or vii-b.
• Z N is selected from:
• Z is selected from
• Z N is as defined above for Z.
• R is hydrogen.
• R N is unsubstituted Cl-4 alkyl.
• X 2 is selected from -CH 2 -, -CH 2 -
• X 2 is selected from -CH 2 -, -CH(Me)-, -CH 2 -
• X 2 is -CH 2 - .
• T N is an -optionally substituted, saturated, unsaturated, or aromatic 8-12 membered bicyclic ring.
• T N is selected from 1-pyrrolyl, 2, 3-dihydro-lH-pyrrol-l-yl, 1-pyrazolyl, 1-imidazolyl, 1- pyrrolidinyl, 1 , 2 , 3 , 4-tetrahydropyrid-l-yl, 1,2,3,6- tetrahydropyrid-1-yl, 1-piperidinyl, 1-piperazinyl, 1- morpholinyl, 1-azepinyl, 1-azepanyl, 1-indolyl, 1-indolinyl, 1, 2, 3, 4-tetrahydroquinolin-l-yl, 1,2, 3 , 4-tetrahydroisoquinolin- 2-yl, wherein said ring is optionally substituted with up to 3 substituents.
• T N is optionally substituted with up to 3 substituents.
• T N is
• T N is formula i or ii above, optionally substituted as provided above. Or, T N is formula v or vi above, optionally substituted as provided above Or, T N is formula vii, optionally substituted as provided above. [00268] According to another embodiment, T N is an optionally substituted ring selected from: lll IX xi ll
• T N is any of the above rings viii to xxiii, optionally fused to an optionally substituted phenyl ring.
• T N is any of the above rings viii to xxiii, optionally fused to an optionally substituted 6-membered aromatic heterocyclic ring having up to 3 nitrogen atoms. Preferred such 6-membered rings include pyridyl, pyrimidinyl, pyrazyl, or pyridazinyl .
• T is an optionally substituted ring selected from:
• T N is any of the above rings xxiii to xxx, optionally fused to an optionally substituted 6-membered aromatic heterocyclic ring having up to 3 nitrogen atoms.
• 6-membered rings include pyridyl, pyrimidinyl, pyrazyl, or pyridazinyl.
• Preferred substituents on T N are independently selected from halo, cyano, trifluoromethyl, OH, C ⁇ - 4 alkyl, C 2 - 4 alkenyl,
• C ⁇ - 4 alkoxy trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C ⁇ -4 alkyl), N(C 1-4 alkyl) 2 , NHC(0)d- 4 alkyl, or C(0)C ⁇ - 4 alkyl.
• the phenylene ring attached to the sulfonyl group is optionally substituted with up two substituents selected from halo, cyano, trifluoromethyl, OH, C ⁇ - 4 alkyl, C 2 - 4 alkenyl, C ⁇ - 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 ,
• the present invention provides compounds wherein: a. Z N is thiazol-2-yl; b. R N is hydrogen; c. X 2 is Cl-4 alkylene, preferably, -CH 2 - or -CH 2 -CH 2 -; and d.
• T N is selected from indol-1-yl, 1,2,3,4- tetrahydroquinolin-1-yl , indolin-1-yl, 1,2,3,4- tetrahydroisoquinolin-2-yl , or 5-benzylidene-thiazolidin- 2 , 4-dione-3-yl, optionally substituted with up to three substituents independently selected from Cl-4 alkyl, Cl-4 alkoxy, halo, trifluoromethyl, or cyano.
• the present invention provides compounds wherein: a. Z N is thiazol-2-yl ; b. R N is hydrogen; c.
• the present invention provides compounds wherein: a. Z N is thiazol-2-yl; b. R N is hydrogen; c. X 2 is Cl-3 alkylene, preferably -CH 2 -; d.
• T N is selected from indol-1-yl, 1,2,3,4- tetrahydroisoquinolin-2-yl, 5-methyl-indol-1-yl , 6- chloroindolin-1-yl, 6-chloro-indol-l-yl, 6-fluoro-indol-1- yl, 6-chloro-1, 2 , 3 , 4-tetrahydroquinolin-l-yl, 4-fluoro- indol-1-yl, 5-fluoro-indol-1-yl , 4, 4-difluoropiperidinyl, 5-cyano-indol -1-yl, 5-ethyl-indol-1-yl, 1,2,3,4- tetrahydroquinolin-1-yl, 6-trifluoromethyl-indol -1-yl , 5,6- dimethoxy-indol-1-yl, 6-fluoro-1, 2, 3 , 4-tetra
• the present invention provides a compound of formula IV:
• R 1 and R 2 wherein Z M and T M each is independently and optionally substituted with up to 4 substituents independently selected from R 1 , R 2 , R 3 , R 4 , or R 5 ;
• Y is halo, CN, N0 2 , CF 3 , OCF3 , OH, SR 6 , S(0)R 6 , S0 2 R 6 , NH 2 ,
• R 2 is aliphatic, wherein each R 2 is optionally substituted with up to 2 substituents independently selected from R-*-, R 4 , or 5 ;
• R 3 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring is optionally substituted with up to 3 substituents, independently selected from R 1 , R 2 , R 4 or R 5 ;
• R 4 is OR 5 , OR 6 , OC(0)R 6 , OC(0)R 5 , OC(0)OR 6 , OC(0)OR 5 , OC(0)N(R 6 ) 2 , OC(0)N(R 5 ) 2 , OC (O) N (R 6 R 5 ) , OP(0) (OR 6 ) 2 , OP (O)
• NR 6 S0 2 R 6 NR 6 S0 2 R 5 , NR 5 S0 2 R 5 , NR 6 S0 2 N(R 6 ) 2 , NR 6 S0 2 NR 5 R 6 , NR 6 S0 2 N(R 5 ) 2 , NR 5 S0 2 NR 5 R 6 , NR 5 S0 2 N (R 5 ) 2 , N(OR 6 )R 6 , N(OR 6 )R 5 , N(OR 5 )'R 5 , N(OR 5 )R 6 , P(O) (OR 6 )N(R 6 ) 2 , P (0) (OR 6 ) N (R 5 R 6 ) , P(O) (OR 6 )N(R 5 ) 2 .
• R 5 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring is optionally substituted with up to 3 R ⁇ - substituents
• R 6 is H or aliphatic, wherein R 6 is optionally substituted with a R 7 substituent
• R 7 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring and each R 7 is optionally substituted with up to 2 substituents independently chosen from H, aliphatic, or (CH 2 )n ⁇
• Z' is selected from halo, CN, N0 2 , C (halo) 3, CH(halo) 2 ,
• N (aliphatic) R 8 COOH, C (0) O ( -aliphatic) , or O-aliphatic; and R 8 is an amino protecting group.
• R 8 is an amino protecting group.
• the following compounds are excluded: (a) when Z is optionally substituted pyrimidinyl or thiazolyl, both R 6 are hydrogen, and XI is NH, then T is not optionally substituted adamantyl; (b) when Z is optionally substituted pyridyl, pyrimidinyl, isoxazolyl, or thiazolyl, both R 6 are hydrogen, and X x is NH,
• T is not optionally substituted with up to two halo atoms; (c) when both R 6 are hydrogen, and Xi is NH, then T is not 1-naphthyl, 2 -naphthyl, or 7-hydroxynaphth-l-yl; (d) when Z is pyrimidinyl, 5-methylisoxazolyl, or pyridyl, both R 6 are hydrogen, and Xi is NH, then T is not subtituted purinyl ; and (e) when Z is thiazol-2-yl, both R 6 are hydrogen, and Xi is NH, then T is not substituted 3H-isobenzofuran-l-one-7-yl .
• X 1 is 0.
• X 1 is S.
• XI is
• each R N is independently hydrogen.
• Z M is selected from:
• Z M is selected from : i-a i-b or i-c .
• Z M is formula i-a.
• Z M is selected from:
• Z M is selected from: v-a v-b or v-c .
• Z M is selected from: ii-a ii-b or iii-a .
• Z M is selected from: vi-a vii-a or vii-b .
• Z M is selected from:
• Z M is selected from: ix-a ix-b or ix-c .
• Z M is as defined above for Z.
• R N is hydrogen. Or, R N is unsubstituted Cl-4 alkyl.
• Z M is an optionally substituted
• Xi is NH.
• X x is O.
• T M is phenyl or naphthyl, optionally substituted with up to 3 substituents independently selected from halo, cyano, trifluoromethyl, OH, C ⁇ _ 4 alkyl, C 2 - 4 alkenyl, C ⁇ _ 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C ⁇ -4 alkyl), N(C ⁇ - 4 alkyl) 2 , NHC (O) d-4 alkyl, 1-pyrrolidinyl , 1- piperidinyl, 1-morpholinyl, or C(0)C ⁇ - 4 alkyl.
• T M is selected from:
• T M is optionally substituted with up to three substituents independently selected from halo, cyano, trifluoromethyl, OH, C ⁇ _ 4 alkyl, C 2 - alkenyl, C ⁇ _ 4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C ⁇ _ 4 alkyl), N(d- 4 alkyl) 2 , NHC (O) d-4 alkyl, or C (O) C_._ alkyl. [00298] Or , T is selected from:
• T M is optionally substituted with up to three substituents independently selected from halo, cyano, trifluoromethyl, OH, C. 4 alkyl, C 2 - 4 alkenyl, C ⁇ -4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH 2 , NH(C 1 _ 4 alkyl), N(C 1 . 4 alkyl) 2 ,
• NHC (0) C ⁇ - 4 alkyl or C (0) C ⁇ - 4 alkyl .
• the substituents are independently selected from oxo, halo, cyano, trifluoromethyl,
• X 2 is -CH 2 -; -CH 2 -CH 2 - or -CH 2 CH 2 CH 2 -; b. Xi is 0 or S; and c.
• T is selected from 8-tri luoromethylquinolin-4-yl, 3- chloro-4-fluorophenyl, 1-naphthyl, 4 -chloro-3 -fluorophenyl , 6-fluoro-2-methyl-quinolin-4-yl, 2 , 4-dichlorophenyl, 4- chlorophenyl, 2 , 3 -difluorophenyl, 2 -chloro-4 -methoxyphenyl , 4-trifluoromethylpehnyl , 4-chloro-2 -fluorophenyl , benzo [1, 3] oxathiol-2-one-6-yl, l-phenyl-tetrazol-5-yl, benzo [1,2,5] oxadiazol-5-yl, 3-cyano-5, 6,7,8- tetrahydroquinolin-2-yl, quinolin-2-yl , isoquinolin-5-yl, quinolin
• T is selected from benzo [b] thiophen-3-yl, 5-chloro- benzo [b] thiophen-2-yl, 5-chloro-2 , 3-dihydro-lH-indol-1-yl , 5-fluoro-2,3-dihydro-IH-indol-1-yl , 8-methoxy-1,2,3,4- tetrahydronaphth-2-yl, 1,2,3 , 4-tetrahydroquinolin-l-yl, 6 , 7-dimethoxy-l , 2 , 3 , 4-tetrahydroisoquinolin-2-yl , 2,3- dihydro-IH-indol-1-yl, 1,2,3,4-tetrahydroisoquinolin-2-yl , 2-methyl-2 , 3 -dihydro-IH-indol-1-yl, 6-methoxy-1,2,3,4- tetrahydroquinol
• T is selected from 4, 6-dichloroindol-2-yl, benzofuran-2-yl, 1-naphthyl, 2- methyl-6-fluoroquinolin-4-yl, 5-fluoro-indol-2-yl, 5- chlorothiophen-2-yl, benzopyran-3-yl, 3-bromo-4-methylphenyl, 2- (furan-2-yl) -quinolin-4-yl, N-methyl-5-trifluoromethoxy-indol-2- yl, benzothiophen-3 -yl, 5-fluoro-benzothiophen-2-yl, 2-methyl- quinolin-4-yl, 6-chloro-indol-2-yl, 6-bromo-indol-2-yl , 2- phenyl-5-methyl-l, 2-oxazol-3-yl, N, 6-dimethyl-indol
• X 2 is CH 2 , -CH2CH2, or CH 2 CH 2 CH 2 ; c. T is phenyl optionally substituted with up to three substituents selected from halo, cyano, trifluoromethyl, OH, C ⁇ - 4 alkyl, C1-4 alkoxy, trifluoromethoxy, C(0)NH 2 , NH2 , NH(Cl-4 alkyl), N(Cl-4 alkyl)2, NHC(0)Cl-4 alkyl, 1- pyrrolidinyl, 1-piperidinyl, 1-morpholinyl, or C (O) C 1 - 4 alkyl .
• Xi is O.
• Xi is S.
• X x is
• X 2 is CH 2 / -CH 2 CH 2 , or CH 2 CH 2 CH 2 ; b . X x is O, S , or NH; and c.
• T is quinolin-4-yl, quinolin-5-yl, quinolin-6-yl, quinolin-7-yl, quinolin-8-yl , isoquinolin-1-yl , 1-naphthyl, 2- naphthyl, 5a, 6,7,8,9, 9a-hexahydro-dibenzofuran-2-yl, benzo [1, 3] dioxol-6-yl, benzothiazol-5-yl , indan-l-one-4-yl, benzo [1, 2, 5] oxadiazol-4-yl, indol-4-yl, 4-methyl-chromen-2-one- 7-yl, indol-5-yl, benzo- [1, 2 , 3] -triazin-4-yl , or benzimidazol-2- yl, wherein T is optionally substituted with up to three substituents selected from halo, cyano, trifluoromethyl , OH
• X 2 is CH 2 , -CH 2 CH , or CH 2 CHCH 2 ;
• T is quinolin-5-yl, 2-naphthyl, 5a, 6, 7 , 8 , 9, 9a- hexahydro-dibenzofuran-2-yl, benzo [1, 3] dioxol-6-yl , 8- fluoroquinolin-4-yl , 2-methyl-benzothiazol-5-yl, 7- trifluoromethyl-quinolin- -yl , indan-1-one-4-yl , benzo [1, 2 , 5] oxadiazol-4-yl , isoquinolin-1-yl , indol-4-yl, 5, 7-dichloro-2-methylquinolin-8-yl , 7-chloro-quinolin-4-yl , 4-methyl-chromen-2-one-7-
• the present invention provides a compound having formula (V) : T 1 --L 11 —A--L 2 —Z; wherein: Ti is a 8-14 membered aromatic or non-aromatic bicyclic or tricyclic ring, having 0-5 heteroatoms selected from O, S, N, NH, S(O) or S0 2 ; LU is -(X 1 ) p -(CHRl) r -(X 2 )-Ry; wherein: p is 0 or 1; r is 0 or 1; Xl is 0, S, or NRx, wherein R x is H or R 2 ; X 2 is R 2 ; Ry is -C(0) -NR 2 -; L 22 is 0C(0), C(0)0, S(0), S0 2 , N(R 5 )S0 2 , N(R 6 )S0 2 ,
• A is a 5-7 membered monocyclic aromatic ring, having 0-4 heteroatoms
• Z is 2 -thiazolyl; wherein each of T x , A, and Z is optionally substituted with up to 4 suitable substituents independently selected from R 1 , R 2 , R 3 , R 4 , or R 5 ;
• Y is halo, CN, N0 2 , CF 3 , OCF 3 , OH, SR 6 , S(0)R 6 , S0 2 R 6 , NH 2 ,
• R 2 is aliphatic, wherein each R 2 is optionally substituted with up to 2 substituents independently selected from R ⁇ , R 4 , or
• R 3 is a cycloaliphatic, aryl, heterocyclic, or heteroaryl ring is optionally substituted with up to 3 substituents, independently selected from R 1 , R 2 , R 4 or R 5 ;
• R 4 is OR 5 , OR 6 , OC(0)R 6 , OC(0)R 5 , OC(0)OR 6 , OC(0)OR 5 , OC(0)N(R 6 ) 2 .
• L 22 is S0 2 , N(R 5 )S0 2 , N(R 6 )S0 2 , S0 2 N(R 5 ), S0 2 N(R 6 ),
• u , L 22 , R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , and R 8 in formula (V) are as described above for formula (I) .
• Ry is -C(0)-NR 2 -.
• T]_ is a 8-14 membered aromatic or non-aromatic bicyclic or tricyclic ring, having 0 heteroatoms. More preferably, ] _ is naphthyl. Or, T j _ is anthracenyl . According to an alternate more preferred embodiment, T ⁇ is tetralinyl or decalinyl.
• Ti is a 8-14 membered non-aromatic tricyclic ring, having up to 5 heteroatoms.
• Ti is a 8-14 membered aromatic tricyclic ring, having up to 5 heteroatoms.
• Exemplary tricyclic rings include dibenzofuranyl , carbazolyl, acridinyl, phenazinyl, phenothiazinyl , phenoxazinyl, acridinyl, phenazinyl, phenothiazinyl, phenoxainyl, or carbazolyl.
• A is phenyl .
• A is a 5-6 membered monocyclic aromatic ring having 1-4 heteroatoms. More preferably, A is 5-6 membered monocyclic aromatic ring having 1-3 heteroatoms.
• Exemplary rings include thiazolyl, isothiazolyl, thiadiazolyl, thiaphenyl, furanyl, oxazolyl, isooxazolyl, oxadiazolyl, triazolyl, imidazolyl, pyrazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, or pyrrolyl.
• FIGURE 1 recites exemplary compounds of the present invention.
• step a The coupling of i with anilines (step a) using CDI and DMA under refluxing conditions, or HATU and TEA in pyridine under mircrowave conditions at 200°C, or isobutyl chlorocarbonate and TEA in DCM yields ii.
• step b Reaction of intermediate ii with C1S0 3 H (step b) under refluxing conditions gives iv.
• step c Reaction of ii C1S0 3 H at 0°C (step c) gives intermediate iii.
• Coupling of intermediate i with aminosulfonic acids (step d) using HATU and TEA in pyridine under mircrowave conditions at 200°C, or BOP and TEA in CH 3 CN at rt yields iii.
• reaction of intermediate iii with S0 2 C1 yields iv.
• reaction of intermediate iii with cyanuric chloride and TEA in acetone under microwave conditions at 120°C provides iv.
• reaction of iv with various amines in pyridine at room temperature yields IA.
• Reaction of i and ii (step a) in pyridine and DCM at rt yields iv.
• the coupling of i and iii (step b) using CDI and DMA under refluxing conditions, or HATU and TEA in pyridine under mircrowave conditions at 200°C, or BOP and TEA in CH 3 CN at rt yields iv.
• the reaction of iv and v (step c) under alkylation conditions provides IIA. These alkylation conditions include NaH and K 2 C0 3 as bases, DMF, DMSO, and THF as solvents, under rt, microwave, and reflux conditions.
• Scheme F Scheme F provides useful intermediates for Scheme A-C, E.
• the reaction of i and ii (step a) under alkylation conditions provides intermediate iii.
• These alkylation conditions include NaH and K 2 C0 3 as bases, and Nal can be added.
• Solvents include DMF, DMSO, and THF, and reaction conditions include rt , microwave, and refluxing conditions.
• the reaction of i and ii (step c) in H 2 0 and NaOH provides intermediate iv.
• the reaction of intermediate iii (step b) using 2N NaOH, or H 2 0 in DMA under microwave conditions yields iv.
• Treatment of iv with oxalyl chloride or thionyl chloride provides v.
• Scheme G provides a synthesis to compounds of Formula IIB.
• step a The coupling of i and ii (step a) using CDI and DMA under refluxing conditions, or HATU and TEA in pyridine under mircrowave conditions at 200°C, or BOP and TEA in CH 3 CN at rt yields IIB. Reaction of i and iii (step a) in pyridine and DCM at rt yields IIB.
• Scheme H provides an alternative synthesis to compounds of Formula IIB.
• step a The coupling of i with anilines (step a) using CDI and DMA under refluxing conditions, or HATU and TEA in pyridine under mircrowave conditions at 200°C, or isobutyl chlorocarbonate and TEA in DCM yields ii.
• step b Reaction of intermediate ii with C1S0 3 H (step b) under refluxing conditions gives iv.
• step c Reaction of ii CISO 3 H at 0°C (step c) gives intermediate iii.
• step d Coupling of intermediate i with aminosulfonic acids (step d) using HATU and TEA in pyridine under mircrowave conditions at 200°C, or BOP and TEA in CH 3 CN at rt yields iii.
• reaction of intermediate iii with S0 2 C1 yields iv.
• reaction of intermediate iii with cyanuric chloride and TEA in acetone under microwave conditions at 120°C provides iv.
• reaction of iv with various amines step f) in pyridine at room temperature yields IIB.
• step a The coupling of i and ii (step a) using CDI and DMA under refluxing conditions, or HATU and TEA in pyridine under mircrowave conditions at 200°C, or BOP and TEA in CH 3 CN at rt yields IIC. Reaction of i and iii (step a) in pyridine and DCM at rt yields IIC.
• Scheme J provides an alternative synthesis for compounds of Formula IIC.
• step a The coupling of i with anilines (step a) using CDI and DMA under refluxing conditions, or HATU and TEA in pyridine under mircrowave conditions at 200°C, or isobutyl chlorocarbonate and TEA in DCM yields ii .
• step b Reaction of intermediate ii with ClS0 3 H (step b) under refluxing conditions gives iv.
• step c Reaction of ii CISO 3 H at 0°C (step c) gives intermediate iii.
• step d Coupling of intermediate i with aminosulfonic acids (step d) using HATU and TEA in pyridine under mircrowave conditions at 200°C, or BOP and TEA in CH 3 CN at rt yields iii.
• step e Reaction of intermediate iii with S0 2 C1 (step e) yields iv.
• reaction of intermediate iii with cyanuric chloride and TEA in acetone under microwave conditions at 120°C (step e) provides iv. _ Reaction of iv with various amines (step f) in pyridine at room temperature yields IIB.
• Scheme K provides a synthesis for compounds of Formula IID.
• step a The reaction of intermediate i with 20% diphosgene and TEA (step a) in PhCH 3 with heating provides ii.
• step b The treatment of ii with iii (step b) yields IID.
• step a The reaction of intermediate i with ii in TEA/CH 3 CN (step a) provides compounds IID.
• reaction of i and ii under alkylation conditions provides III.
• alkylation conditions include NaH and K 2 C0 3 as bases, and Nal can be added.
• Solvents include DMF, DMSO, and
• reaction conditions include rt, microwave, and refluxing conditions .
• compositions comprising any of the compounds as described herein, and optionally comprise a pharmaceutically acceptable carrier, adjuvant or vehicle.
• these compositions optionally further comprise one or more additional therapeutic agents.
• a pharmaceutically acceptable derivative includes, but is not limited to, pharmaceutically acceptable salts, esters, salts of such esters, or any other adduct or derivative which upon administration to a patient in need is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite or residue thereof.
• the term “pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgement, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio.
• a “pharmaceutically acceptable salt” means any non-toxic salt or salt of .an ester of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof.
• the term “inhibitorily active metabolite or residue thereof” means that a metabolite or residue thereof is also an inhibitor of a voltage-gated sodium ion channel .
• any conventional carrier medium is incompatible with the compounds of the invention, such as by producing any undesirable biological effect or otherwise interacting in a deleterious manner with any other component (s) of the pharmaceutically acceptable composition, its use is contemplated to be within the scope of this invention.
• compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops) , bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated.
• the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect .
• the injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile , water or other sterile injectable medium prior to use.
• sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile , water or other sterile injectable medium prior to use.
• compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating ' excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
• suitable non-irritating ' excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
• Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
• the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar- -agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonit
• Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
• the active compounds can also be in microencapsulated form with one or more excipients as noted above.
• the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art.
• the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch.
• Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches.
• the active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required.
• Ophthalmic formulation, eardrops, and eye drops are also contemplated as being within the scope of this invention.
• the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body.
• Such dosage forms are prepared by dissolving or dispensing the compound in a proper medium.
• Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel .
• the compounds of the invention are useful as inhibitors of voltage-gated sodium ion channels or calcium channels, preferably N-type calcium channels.
• the compounds and compositions of the invention are inhibitors of one or more of NaVl.l, NaVl .2 , NaVl.3, NaVl.4, NaVl .5 , NaVl .6 , NaVl .7 , NaVl .8 , NaVl .9 , or CaV2.2, and thus, without wishing to be bound by any particular theory, the compounds and compositions are particularly useful for treating or lessening the severity of a disease, condition, or disorder where activation or hyperactivity of one or more of NaVl.l, NaVl.2, NaVl .3 , NaVl .4 , NaVl.5, NaVl.6, NaVl.7, NaVl.8, NaVl.9, or CaV2.2 is implicated in the disease, condition, or disorder.
• the present invention provides a method for treating or lessening the severity of a disease, condition, or disorder where activation or hyperactivity of one or more of NaVl.l, NaVl.2, NaVl .3 , NaVl .4 , NaVl.5, NaVl.6, NaVl.7, NaVl.8 , NaVl.9, or CaV2.2 is implicated in the disease state .
• a compound utilized in this invention as an inhibitor of NaVl.l, NaVl.2, NaVl .3 , NaVl .4 , NaVl.5, NaVl.6, NaVl.7, NaVl.8, NaVl.9, or CaV2.2 may be assayed according to methods described generally in the Examples herein, or according to methods available to one of ordinary skill in the art.
• compounds of the invention are useful as inhibitors of NaVl .8.
• compounds of the invention are useful as inhibitors of NaVl .8 and CaV2.2.
• compounds of the invention are useful as inhibitors of CaV2.2.
• additional therapeutic agents that are normally administered to treat or prevent a particular disease, or condition, are known as "appropriate for the disease, or condition, being treated".
• the amount of additional therapeutic agent present in the compositions of this invention will be no more than the amount that would normally be administered in a composition comprising that therapeutic agent as the only active agent.
• the amount of additional therapeutic agent in the presently disclosed compositions will range from about 50% to 100% of the amount normally present in a composition comprising that agent as the only therapeutically active agent.
• compositions for coating an implantable medical device such as prostheses, artificial valves, vascular grafts, stents and catheters.
• the present invention in another aspect, includes a composition for coating an implantable device comprising a compound of the present invention as described generally above, and in classes and subclasses herein, and a carrier suitable for coating said implantable device.
• Glacial acetic acid (1.4 mL) was added to ⁇ pH 4 resulting in precipitation of product. After stirring the suspension for 6 h, the solid was collected by vacuum filtration, rinsed with water, and dryed in a vacuum dessicator over CaCl . The yield of white solid was 0.56 g (87%) .
• N' - (2-thiazolyl) sulfanilamide (0.47 g, 1.0 equiv) was added followed by pyridine (0.30 mL, 2.0 equiv) . The mixture was allowed to warm to rt overnight. The solid was collected and rinsed with fresh DCM. Further purification was effected by suspending the solid in 20 mL methanol, stirring vigorously for 4 h, and filtration. After drying under vacuum, white solid 0.65 g (69%) was obtained.
• Method A To a solution of sulfathiazole (102 mg, 0.40 mmol) and N, N-diisopropylethylamine (0.17 mL, 0.95 mmol) in acetonitrile (10 mL) was added a 20% phosgene solution in toluene (20% w/w in toluene, 1 mL) . The reaction mixture was stirred under rflux for 2 h. The excess of phosgene and solvent were evaporated in vacuo and coevaporated with acetonitrile (5 mL) .
• Compounds of the invention are useful as antagonists of voltage-gated sodium ion channels.
• /Antagonist properties of test compounds were assessed as follows. Cells expressing the NaV of interest were placed into microtiter plates. After an incubation period, the cells were stained with fluorescent dyes sensitive to the transmembrane potential . The test compounds were added to the microtiter plate. The cells were stimulated with either a chemical or electrical means to evoke a NaV dependent membrane potential change from unblocked channels, which was detected and measured with transmembrane potential-sensitive dyes. Antagonists were detected as a decreased membrane potential response to the stimulus.
• the optical membrane potential assay utilized voltage-sensitive FRET sensors described by Gonzalez and Tsien (See, Gonzalez, J. E.
• VIPR ® optical membrane potential assay method with chemical stimulation [00329] Cell Handling and Dye Loading [00330] 24 hours before the assay on VIPR, CHO cells endogenously expressing a NaVl .2 type voltage-gated NaV are seeded in 96-well poly-lysine coated plates at 60,000 cells per well. Other subtypes are performed in an analogous mode in a cell line expressing the NaV of interest.
• a 15 uM CC2-DMPE solution is prepared by mixing 5 mM coumarin stock solution with 10% Pluronic 127 1:1 and then dissolving the mix in the appropriate volume of BS#2.
• the cells are loaded with 80 ⁇ L of the CC2-DMPE solution. Plates are incubated in the dark for 30 minutes at room temperature .
• CC2-DMPE is removed and the cells are washed twice with 225 ⁇ L of BS#2. As before, the residual volume should be 40 ⁇ L.
• the data is further reduced by calculating the initial (Ri) and final (Rf) ratios. These are the average ratio values during part or all of the pre-stimulation period, and during sample points during the stimulation period.
• the response to the stimulus R . R f /Ri is then calculated.
• baseline 2-7 sec and final response is sampled at 15-24 sec .
• DiSBAC 2 (3) prepared as a 12 mM stock in DMSO and stored at -20°C
• ABSC1 prepared as a 200 mM stock in distilled H 2 0 and stored at room temperature
• Pluronic F-127 100 mg/mL Pluronic F-127 (Sigma #P2443) , in dry DMSO 10 mM DiSBAC 2 (3) (Aurora #00-100-010) in dry DMSO 10 mM CC2-DMPE (Aurora #00-100-008) in dry DMSO 200 mM ABSC1 in H 2 0
• Assay buffer #1 140 mM NaCl, .5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM HEPES, 10 mM glucose, pH 7.40, 330 mOsm
• Pluronic stock (1000X) 100 mg/mL pluronic 127 in dry DMSO Oxonol stock (3333X) : 10 mM DiSBAC 2 (3) in dry DMSO Coumarin stock (100OX) : 10 mM CC2-DMPE in dry DMSO ABSC1 stock (400X) : 200 mM ABSC1 in water
• Extracellular solution (in mM-) : NaCl (138) , CaCl 2 (1.26), KCl (5.33), KH 2 P0 4 (0.44), MgCl 2 (0.5), MgS0 4 (0.41), NaHC0 3 (4), Na 2 HP0 4 (0.3), glucose (5.6), HEPES (10), CdCl2 (0.4 ), NiCl2 (0.1), TTX (0.25 x 10 "3 ) .
• HEK293 cells stably expressing CaV2.2 are plated into.96-well microtiter plates. After an appropriate incubation period, the cells are stained with the voltage sensitive dyes CC2-DMPE/DiSBAC2 (3) as follows. Reagents :
• 2X CC2-DMPE 20 ⁇ M CC2-DMPE: 10 mM CC2-DMPE is vortexed with an equivalent volume of 10% pluronic, followed by vortexing in required amount of HBSS containing 10 mM HEPES. Each cell plate will require 5 mL of 2X CC2-DMPE. 50 ⁇ L of 2X CC2-DMPE is added to wells containing washed cells, resulting in a 10 ⁇ M final staining concentration. The cells are stained for 30 minutes in the dark at RT.
• 2X CC2DMPE & DISBAC S (3) 8 ⁇ M CC2DMPE & 2.
• 5X AY17 750 ⁇ M AY17 with 15mM BaCl 2 : Add Acid Yellow 17 to vessel containing Bath X. Mix well. Allow solution to sit for 10 minutes. Slowly mix in 370mM BaCl 2 . This solution can be used to solvate compound plates. Note that compound plates are made at 1.5X drug concentration and not the usual 2X. Wash CC2 stained plate, again, leaving residual volume of 50 ⁇ L. Add 100 uL/well of the AY17 solution. Stain forl5 minutes in the dark at RT. Run plate on the optical reader.
• the electrical stimulation instrument and methods of use are described in ION Channel Assay Methods PCT/USOl/21652, herein incorporated by reference.
• the instrument comprises a microtiter plate handler, an optical system for exciting the coumarin dye while simultaneously recording the coumarin and oxonol emissions, a waveform generator, a current- or voltage-controlled amplifier, and a device for inserting electrodes in well . Under integrated computer control, this instrument passes user- programmed electrical stimulus protocols to cells within the wells of the microtiter plate.
• [00377] Use the current-controlled amplifier to deliver stimulation wave pulses for 3-5 s. Two seconds of pre- stimulus recording are performed to obtain the un- stimulated intensities. Five seconds of post-stimulation recording are performed to examine the relaxation to the resting state. [00378] Data Analysis [00379] Data are analyzed and reported as normalized ratios of background-subtracted emission intensities measured in the 460 nm and 580 nm channels. Background intensities are then subtracted from each assay channel . Background intensities are obtained by measuring the emission intensities during the same time periods from identically treated assay wells in which there are no cells. The response as a function of time is then reported as the ratios obtained using the following formula:
• the data is further reduced by calculating the initial (Ri) and final (Rf) ratios. These are the average ratio values during part or all of the pre-stimulation period, and during sample points during the stimulation period.
• the response to the stimulus R . R f /Ri is then calculated.
• Control responses are obtained by performing assays in the presence of a compound with the desired properties (positive control), such as mibefradii, and in the absence of pharmacological agents (negative control) .
• Positive control such as mibefradii
• negative control negative control
• Responses to the negative (N) and positive (P) controls are calculated as above.
• the compound antagonist activity A is defined as : R — P
• CaV2.2 calcium currents were recorded from HEK293 cells using the whole-cell variation of the patch clamp technique. Recordings were made at room temperature (-22° C) with thick walled borosilicate glass electrodes (WPI ; resistance 3-4 M. using an Axopatch 200B amplifier (Axon Instruments) . After establishing the whole-cell configuration, approximately 15 minutes were allowed for the pipette solution to equilibrate within the cell before beginning recording. Currents were lowpass filtered between 2-5 kHz and digitally sampled at 10 kHz. Series resistance was compensated 60-70% and was monitored continuously throughout the experiment.
• Extracellular solution (in mM) : NaCl (138), BaCl 2 (10), KCl (5.33), KH 2 P0 4 (0.44), MgCl 2 (0.5), MgS0 4 (0.41), NaHC0 3 (4), Na 2 HP0 4 (0.3), glucose (5.6), HEPES (10).

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