Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
  • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
  • C07H15/20—Carbocyclic rings
  • C07H15/24—Condensed ring systems having three or more rings
  • C07H15/252—Naphthacene radicals, e.g. daunomycins, adriamycins
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C43/00—Ethers; Compounds having groups, groups or groups
  • C07C43/30—Compounds having groups
  • C07C43/313—Compounds having groups containing halogen

Definitions

• the invention relates to anthracycline glycosides, to processes for their preparation and to pharmaceutical compositions containing them.
• the invention provides new anthracycline glycosides of general formula A in which the 3'-nitrogen atom is enclosed in a 2-alkoxy-4-morpholinyl ring:
• X represents a linear or branched C 1 -C 6 alkyl group or a benzyl residue -CH 2 C 5 H 5 and which has a (S) or (R) configuration at the 2"-carbon atom, and pharmaceutically acceptable acid addition salts thereof.
• the preferred salt is the hydrochloride salt.
• Morpholino-anthracyclines are well known compounds endowed with promising antitumor activity on experimental murine tumors [see: E.W. Acton in Bioactive Molecules, 55- 101, vol 6, Edited by J.W. Lown, Elveiser 1988].
• the substituted morpholinyl rings are prepared through bis-alkylation of the 3'-amino group of anthracyclines with novel chiral 1,5-diiodo-2- alkoxy or -benzyloxy derivatives that are within the scope of the invention.
• the preferred anthracycline glycosides of general formula A include:
• the compounds may have a (S) or (R) configuration at carbon atom C-2" of the morpholino ring.
• the new anthracycline glycoside antibiotics of the invention i.e. those of general formula A., are prepared by the formation of a substituted morpholinyl ring at C-3' on the sugar moiety of the antitumor anthracycline glycoside doxorubicin (B) :
• the present invention therefore provides a process for the preparation of an anthracycline glycoside of formula A or a pharmaceutically acceptable acid addition salt thereof, which process comprises:
• the alkylation of the C-3' amino group of doxorubicin or the doxorubicin salt is typically performed in step (i) in a polar aprotic solvent and in the presence of a dry organic base such as triethylamine. Reaction is generally carried out at room temperature from eight to twenty four hours.
• the carbon atom C-2 bearing the -OX group in the diiodo compound may have a (S) or (R)
• doxorubicin or its hydrochloride dissolved in a polar aprotic solvent is reacted, at room temperature and in the presence of a dry organic base, with the diiodo compound of general formula C to give the corresponding morpholinyl doxorubicin derivative of formula A which, after purification on a silica gel column using as eluting system methylene chloride-methanol (97:5 v/v), is isolated, by treatment with methanolic anhydrous hydrogen chloride, as its hydrochloride.
• a pure 2"(R)-[(C 1 -C 6 ) alkoxy or benzyloxy]-anthracycline glycoside of formula A or salt thereof, or a pure 2"(S)-[(C 1 -C 6 ) alkoxy or benzyloxy]-anthracycline glycoside of formula A or salt thereof, may therefore be provided according to the present invention.
• the invention also provides a process for the preparation of optically pure diiodo compounds C, starting from sugar precursors such as the compound of general formula S derived from L-arabinose:
• This process comprises:
• the dialdehyde derivatives can be obtained by using
• the resultant dihydro compounds E 1 are sulfonated at the 1- and 5-hydroxyl groups, typically by using ptoluensulfonyl chloride in pyridine at 4°C to give the sulfonyl esters of formula F from which the diiodo
• derivatives C are obtained upon treatment with sodium or potassium iodide in aprotic solvent such as
• compositions comprising a pharmaceutically acceptable carrier or diluent and, as active ingredient, an
• composition may be formulated and administered, for example intravenously, in conventional manner.
• the anthracycline glycosides of formula A and pharmaceutically acceptable acid addition salts thereof are antitumour agents. They may be used to treat a patient with a tumour by administration of a therapeutically effective amount thereof. The compounds can be used to inhibit the growth of a tumour and are non-toxic at therapeutic doses.
• 1-benzyl-,9-L-arabinopyranoside (S1, 3.48 g, 0.0145 mole) was dissolved in water (100 ml) and treated with sodium periodate (5.6 g, 0.026 mole) at 0°C for two hours. Then barium chloride was added and the mixture was brought to pH 7 with barium carbonate, filtered off and washed with water. The aqueous solution was concentrated under reduced pressure to a syrup and extracted with acetonitrile (50 ml). The organic phase was diluted with a mixture of methanol (20 ml) and water (10 ml) and treated with sodium
• R f 0.55 after heating the TLC plate previously sprayed with sulforic acid.
• BIOLOGICAL ACTIVITY OF 3 -deamino-[2"(S)-methoxy-4"- morpholinyl]doxorubicin (A4) and 3'-deamino-[2"(R)- methoxy-4"-morpholinyl]doxorubicin (A5).
• the compounds have been tested in several experimental system in order to ascertain their cytotoxicity and antitumor activity in experimental animals in comparison with parent Doxorubicin.
• the new anthracyclincs result more cytotoxic than the parent drug on LoVo and LoVo Doxorubicin- resistant cell line (LoVo/Dx), Table 1, and are active "in vivo" against doxorubicin-resistant cell lines.

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• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Engineering & Computer Science (AREA)
• Biochemistry (AREA)
• Biotechnology (AREA)
• Genetics & Genomics (AREA)
• Molecular Biology (AREA)
• Chemical Kinetics & Catalysis (AREA)
• General Chemical & Material Sciences (AREA)
• Medicinal Chemistry (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Pharmacology & Pharmacy (AREA)
• Animal Behavior & Ethology (AREA)
• Public Health (AREA)
• Veterinary Medicine (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Saccharide Compounds (AREA)
• Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
• Steroid Compounds (AREA)

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• 1990
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• 1991
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• 1992
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• 1993
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• 1994
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• 1996
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