US20040082528A1 - Use of hederagenin 3-O-alpha-L-rhamnopyranosyl((1-2)-[Beta-D-glucopyranosyl(1-4)]-alpha-L-arabinopyranoside or an extract from pulsatillae radix containing the same as a therapeutic agent for solid tumors - Google Patents

Use of hederagenin 3-O-alpha-L-rhamnopyranosyl((1-2)-[Beta-D-glucopyranosyl(1-4)]-alpha-L-arabinopyranoside or an extract from pulsatillae radix containing the same as a therapeutic agent for solid tumors Download PDF

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US20040082528A1
US20040082528A1 US10/620,709 US62070903A US2004082528A1 US 20040082528 A1 US20040082528 A1 US 20040082528A1 US 62070903 A US62070903 A US 62070903A US 2004082528 A1 US2004082528 A1 US 2004082528A1
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fraction
pulsatillae radix
hederagenin
arabinopyranoside
rhamnopyranosyl
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Song-Bae Kim
Byung-Zun Ahn
Yong Kim
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Publication of US20040082528A1 publication Critical patent/US20040082528A1/en
Priority to US11/107,665 priority Critical patent/US7682638B2/en
Priority to US12/709,907 priority patent/US8075923B2/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
    • A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
    • A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
    • A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/24—Condensed ring systems having three or more rings
    • C07H15/256—Polyterpene radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
    • C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids

Definitions

  • This invention relates to a use of hederagenin 3-O- ⁇ -L-rhamnopyranosyl((1 ⁇ 2)-[ ⁇ -D-glucopyranosyl(1 ⁇ 4)]- ⁇ -L-arabinopyranoside as represented by the following
  • Pulsatillae radix is a dried root of Pulsatilla species belonging to the Ranunculaceae family (Ki Hwan Bae, Korean Medicinal Herbs, 1999). According to the Chinese medicine, Pulsatillae radix is known to have effects of removing heat from the blood and detoxifying. It has also been used as anti-inflammatory, astringent, hemostatic and antidiarrhea agents, and for the treatment of hematochezia, malaria, nosebleed, and bleeding from tooth. Its flower is called as Pulsatillae Flos, and used for the treatment of malaria, or smallpox. Its leaf is called as Pulsatillae Folium, and used for treatment of waist pain, edema, or heart pain. In addition, decoction of Pulsatillae radix was reported to have an antibacterial effect against amoebic dysentery, and a pesticidal effect against Trichomonas.
  • Pulsatillae radix contains about 9% of saponins, and such ingredients as protoanemonin, anemonin, ranunculin, hederagenin, betulinic acid, and oleanolic acid derivatives and their glycosides have been isolated therefrom by now as represented by the following formula (II):
  • the present inventors isolated deoxypodophyllotoxin from medicinal herbs including Anthriscus sylvestris Hoffman, Pulsatillae radix, etc., and found that this substance inhibited the growth of solid tumor cells by inhibiting angiogenesis, and obtained a Korean patent (Korean Patent Number 315,200) for the same.
  • the present inventors carried out extensive studies to develop an antitumor agent from medicinal herbs. As a result, they obtained a fraction which is poorly soluble in an organic solvent, but is readily soluble in water from Pulsatillae radix, and isolated an antitumor compound from the fraction, and so completed the present invention.
  • the purpose of the present invention is to provide a therapeutic agent for solid tumors comprising an antitumor compound isolated from Pulsatillae radix or a fraction from Pulsatillae radix containing the-same as an active ingredient.
  • One aspect of the present invention provides a therapeutic agent for solid tumors comprising a Pulsatillae radix extract containing hederagenin 3-O- ⁇ -L-rhamnopyranosyl(1 ⁇ 2)-[ ⁇ -D-glucopyranosyl(1 ⁇ 4)]- ⁇ -L-arabinopyranoside as an active ingredient.
  • Solid tumors refer to any mass tumor except blood cancers, a representative example of which is lung tumor.
  • the Pulsatillae radix extract containing hederagenin 3-O- ⁇ -L-rhamnopyranosyl(1 ⁇ 2)-[ ⁇ -D-glucopyranosyl(1 ⁇ 4)]- ⁇ -L-arabinopyranoside can be obtained by extracting Pulsatillae radix with an aqueous solution of ethanol, and forming precipitates by adding acetone thereto to obtain a water-soluble fraction (WT).
  • WT water-soluble fraction
  • Another aspect of the present invention provides a therapeutic agent for solid tumors comprising hederagenin 3-O- ⁇ -L-rhamnopyranosyl(1 ⁇ 2)-[ ⁇ -D-glucopyranosyl(1 ⁇ 4)]- ⁇ -L-arabinopyranoside as an active ingredient.
  • This compound is hederagenin 3-O- ⁇ -L-rhamnopyranosyl(1 ⁇ 2)-[ ⁇ -D-glucopyranosyl(1 ⁇ 4)]- ⁇ -L-arabinopyranoside, and exhibits higher antitumor activity against solid tumors formed with mouse lung tumor cells, LLC (Lewis Lung Carcinoma) cells, or human lung tumor cells, NC1-H23 cells, than a clinical drug, adriamycin.
  • the present process for preparing an antitumor fraction from Pulsatillae radix and isolating an antitumor substance therefrom is as follows.
  • Pulsatillae radix powder was extracted with 50% aqueous solution of ethanol, and dried under reduced pressure. To the obtained dried material was added acetone at 5 to 10-fold amount. The mixture was shaken, centrifuged at 3,000 rpm, and the supernatant was decanted therefrom to obtain an insoluble part. The above process was repeated twice. The remaining insoluble part was readily soluble in water, and so designated as “fraction WT”. This fraction showed relatively high antitumor activity against BDF1 mice transplanted with LLC cells and nude mice transplanted with NCIH23 cells.
  • fraction WT A given amount of fraction WT is dissolved in a given amount of aqueous solution of methanol at various concentrations, and then fractionated on Sephadex LH20 column stabilized with the same solvent.
  • the best isolation is achieved with employing 80% aqueous solution of methanol, and the suitable size of the filled column was 60 ⁇ 4 cm for 500 mg of fraction WT.
  • fraction SPX1 test tube numbers 26-66
  • SPX2 test tube numbers 66-91
  • SPX3 test tube numbers 91-111
  • SPX4 test tube numbers 111-138
  • the fraction SPX3 When spraying sulfuric acid onto the fractions developed on a silica gel plate and heating the plate, the fraction SPX3 developed red color at first, and blue color with the lapse of time, and contains a spotted compound having R f of 0.48 to 0.50 as a main ingredient. It was shown to have high antitumor activity on BDF1 mice transplanted with LLC cells and nude mice transplanted with NCI-H23 cells.
  • SB365 an antitumor substance from the fraction SPX3 showing antitumor activity
  • HPLC HPLC was carried out to obtain pure compound, SB365.
  • SB365 Lieberman-Burchard reaction, IR, 1 H-NMR, 13 C-NMR, and ethanol/sulfuric acid hydrolysis were carried out.
  • SB365 was confirmed as hederagenin 3-O- ⁇ -L-rhamnopyranosyl(1 ⁇ 2)-[ ⁇ -D-glucopyranosyl(1 ⁇ 4)]- ⁇ -L-arabinopyranoside, which is a saponin ingredient that had already been isolated from Pulsatillae radix.
  • Pulsatillae radix extracts according to the present invention or pure SB365 compound isolated therefrom have a weak cytotoxicity against solid tumor cells, but unexpectedly showed excellent antitumor activity in animal experiments. Thus, they can improve problems caused by previous antitumor agents on clinical use, e.g. reducing immune responses due to decreasing blood cells. It is also anticipated that they show low toxicity on rapidly dividing cells like hematopoietic cells, etc.
  • Pulsatillae radix fractions and SB365 according to the present invention may be combined with pharmaceutically acceptable carriers that are conventionally used, and manufactured into various formulations that are conventional in the pharmaceutical field, for example, orally administrable formulations like solutions, suspensions, etc.; injectable formulations like injectable solution or suspension, ready-to-use injectable dry powder, etc.; and topically administrable formulations like ointments, creams, and solutions.
  • pharmaceutically acceptable carriers that are conventionally used, and manufactured into various formulations that are conventional in the pharmaceutical field, for example, orally administrable formulations like solutions, suspensions, etc.; injectable formulations like injectable solution or suspension, ready-to-use injectable dry powder, etc.; and topically administrable formulations like ointments, creams, and solutions.
  • the active ingredient of the present invention is soluble in water, and may be dissolved in various solutions such as physiological saline, Ringer's solution, and nutrient solution, etc.
  • Such pharmaceutical formulations may be intra
  • a recommended dosage of the active ingredient of the present invention to human beings is 3.5 ⁇ 8.0 mg/kg body weight in case of SB365, 20 ⁇ 40 mg/kg body weight in case of fraction SPX3, or 200 ⁇ 300 mg/kg in case of fraction WT.
  • the optimal dosage is 6.5 mg/kg body weight in case of SB365, 25 mg/kg body weight in case of fraction SPX3, or 250 mg/kg body weight in case of fraction WT.
  • such dosage may be appropriately adjusted depending on age, body weight, health, severity of disease of patients.
  • FIG. 1 shows silica gel TLC patterns of fraction WT
  • FIG. 2 shows silica gel TLC patterns of fraction SPX3 purified on a Sephadex LH20 column
  • FIG. 3 is an HPLC chromatogram of fraction SPX3;
  • FIG. 4 is an HPLC chromatogram of SB365.
  • Pulsatillae radix powder of 50 g was extracted three times with 500 ml of 50% aqueous solution of ethanol, and the extract was dried under reduced pressure to obtain 22 g of dried materials.
  • To this dried materials was added 300 ml of acetone, and the mixture was shaken and centrifuged at 3,000 rpm. The supernatant was removed therefrom to give a precipitate.
  • the acetone treatment was repeated twice. The acetone layer was discarded, and an insoluble part was dried to obtain 17.8 g of dried materials (fraction WT).
  • fraction WT was subjected to silica gel TLC (developing solvent:butanol:acetic acid:water in the ratio of 4:1:1, color reaction: sulfuric acid-spraying followed by heating).
  • silica gel TLC developing solvent:butanol:acetic acid:water in the ratio of 4:1:1, color reaction: sulfuric acid-spraying followed by heating.
  • FIG. 1 a blue spot having the R f in the range of 0.48 to 0.50 corresponds to the active ingredient of the present invention as described below.
  • fraction WT showed relatively high antitumor activity (inhibition rate of tumor growth: 57%) on BDF1 mice transplanted with LLC cells.
  • Fraction WT of 560 mg was further fractionated on Sephadex LH20 column (200 g, 60 ⁇ 4 cm) using a mixed solution of methanol and water (80:20) with the flow rate of 1 ml/min, and the fraction volume of 0.5 ml/tube. These fractions were spotted on a silica gel thin layer in order, and developed to obtain factions (developing solvent:butanol:acetic acid:water in the ratio of 4:1:1, color reaction: sulfuric acid-spraying followed by heating). The result is shown in FIG. 2. In FIG.
  • SPX1 (139 mg, 24.8%) was obtained by collecting test tube numbers 26 to 66, and consisted of 4 major spots, lower one of which developed yellow color upon reacting with sulfuric acid.
  • SPX2 (344 mg, 61.4%) was obtained by collecting test tube numbers 66 to 91, and consisted of 2 major spots.
  • SPX3 (61 mg, 10.9%) was obtained by collecting test tube numbers 91 to 111, and developed red color at first, and then blue color with the lapse of time, upon spraying sulfuric acid followed by heating.
  • Fraction SPX3 contained a spot having the R f value in the range of 0.48 to 0.50 as its major ingredient.
  • SPX4 (15.7 mg, 2.8%) was obtained by collecting test tube numbers 111 to 138. Fractions SPX3 and SPX4 had relatively high purity showing one spot on the thin layer.
  • SPX3 exhibited 60% of the inhibition rate of tumor growth on 15 days from its administration.
  • SPX1, SPX2, and SPX4 did not exhibit any action, and so it could be assumed that the substance developing blue color against sulfuric acid was an ingredient with antitumor activity.
  • This SPX3 fraction may be used as an antitumor agent in itself.
  • Reverse-phased silica gel (RP-C 18 , 250 ⁇ 10 mm, manufactured by Metachem) was used as the fixed phase, and a mixed solution of methanol and water (80:20) was used as the mobile phase.
  • the detection wavelength was 210 nm, and the flow rate was 1 ml/min.
  • FIG. 3 As shown in FIG. 3, SPX3 consisted of 3 major substances. From the obtained fractionated amounts, peaks at R t of 8.5 min and 10.4 min contained small amounts of ingredients, and a peak at R t of 23.3 min contained the major ingredient. Thus, it was assumed that the latter would have antitumor activity.
  • SB365 exhibited 81% and 82.1% of the inhibition rate of tumor growth on BDF1 mice transplanted with LLC cells and nude mice transplanted with NC1-H23 cells, respectively, which could be said to be excellent antitumor activities.
  • SB365 isolated in the above was the-white amorphous form with m.p. 239 ⁇ 241° C. and [ ⁇ ] D +23.6° (c, 0.2, MeOH), and was positive in Liebermann-Buchard reaction, and so confirmed as a glycoside.
  • IR cm ⁇ 1
  • peaks were observed at 3400 (br, —OH), 2940 (br, C—H), 1695 (C ⁇ O), 1455, and 1040 (C—O). It was also assumed as a glycoside from the absorption peaks in the ranges of 1000-1100 and 3000-3400.
  • the compound was hydrolyzed in ethanol/sulfuric acid to identify its sugar groups and the structure of aglycone.
  • SB365 was confirmed as hederagenin after comparing physicochemical data of the hydrolysis product, aglycone, 13 C-NMR, and 1 H-NMR data. Further, the hydrolyzed sugars were confirmed as rhamnose, arabinose, and glucose by comparative TLC.
  • SB365 was confirmed as hederagenin 3-O- ⁇ -L-rhamnopyranosyl((1 ⁇ 2)-[ ⁇ -D-glucopyranosyl(1 ⁇ 4)]- ⁇ -L-arabinopyranoside.
  • the mouse species used in this experiment was BDF1, and healthy male mice with the body weight of 18 ⁇ 25 g were used. These animals were supplied with water and foods ad libitum at a place of controlled temperature in the range of 23 ⁇ 24° C., and were bred with an antibiotic-free mouse feed. LLC cells were subcutaneously cultured in C57BL/6 mice for 14 days. A LLC cell-containing tissue was taken and thereto was added sterilized cold physiological saline water (5 ml/g tissue) to prepare a cell suspension. The cell suspension of 0.2 ml was subcutaneously transplanted to the groin region of BDF1 mouse.
  • mice From 24 hours after transplantation, the above mice were divided into several groups consisting of 5 mice. Then, samples, fractions WT and SPX fractions, and SB365, were dissolved in physiological saline, and were injected intraperitoneally at each concentration of 280 mg/kg (WT), 70 mg/kg (SPX1), 171 mg/kg (SPX2), 30.5 mg/kg (SPX3), 8.1 mg/kg (SPX4), and 6.4 mg/kg (SB365). To the negative control group was injected only the physiological saline, and to the positive control group was injected adriamycin (0.5 mg/kg). The injection was scheduled from 24 hours after tumor transplantation to administer the samples successively once a day for 7 days, and stopped for one day, and then, was carried out for 6 more consecutive days.
  • WT weight/kg
  • SPX1 70 mg/kg
  • SPX2 171 mg/kg
  • SPX3 30.5 mg/kg
  • SPX4 8.1 mg/kg
  • SB365
  • mice were weighed twice a week. Antitumor activity was calculated after measuring tumor volume of the control and test groups on 14th and 15th day after sample administration as follows:
  • Tumor volume( mm 3 ) length( mm ) ⁇ width 2 ( mm 2 )/2
  • Inhibition rate of tumor growth(%) ( C ⁇ T ) ⁇ 100 /C
  • fractions WT and SPX3 showed the inhibition rate of tumor growth of 55% and 60%, respectively, and SB365 showed the inhibition rate of tumor growth of 79%, higher than adriamycin of 64% on 15th day from transplantation of tumor cells.
  • mice of the age of 5 weeks weighing 16 ⁇ 25 g obtained from Harlan Co. (USA) were used as experimental animals in this experiment.
  • the mice were used after acclimation for 1 week in an aseptic animal room.
  • the animal room maintained the temperature of 22 ⁇ 2° C., the humidity of 55 ⁇ 5%, and the light and darkness cycle of 12 hours, which was automatically controlled.
  • Solid feed for experimental animals was radiosterilized, and drinking water was sterilized in an autoclave.
  • the animals were supplied with feed and drinking water ad libitum.
  • a human tumor cell line provided by National Cancer Institute (NCI), USA, and preserved in the Korean Research Institute of Bioscience and Biotechnology (KRIBB), Korea, was used.
  • Lung tumor cells NC1-H23 cells, among the human tumor cells, were transplanted to the nude mice.
  • the tumor cells of 3 ⁇ 10 7 cells/ml were subcutaneously transplanted to the mice at a volume of 0.3 ml/20 g body weight.
  • the samples were intraperitoneally injected to the mice every day for 13 days, that is, from 1 day to 14th day except 8th day after tumor cells transplantation.
  • the size of tumor formed during the injection was measured in each animal, and any change in its body weight was also measured.
  • the nude mice were sacrificed, and the tumor was separated and weighed.
  • SB365 of 6.4 mg/kg showed a high inhibition rate of tumor growth, 82.1%, on 16th day after tumor cells transplantation.
  • Tumor cells A549, SK-MEL-2, and MCF-7 were obtained from the KRIBB, and used in this experiment.
  • a culture medium was prepared by adding one pack of L-glutamine-containing RPM11640 medium, 100 ml of fetal bovine serum (FBS) inactivated by heating at a water bath of 50° C. for 30 minutes, 2 g of NaHCO 3 , 100,000 units of penicillin, and 100 mg of streptomycin, to a sterilized distilled water for injection, adjusting the pH of the mixture with 0.1 N HCl to a total volume of 1 l, and disinfecting the mixture with filtration, and stored at 4° C. before use.
  • the cells were maintained by propagation once every three days, and a solution containing 0.5% trypsin and 2% EDTA in physiological buffered saline (PBS) was used to detach the cells from wells.
  • PBS physiological buffered saline
  • SRB Sulforhodamine-B
  • the cells were detached from wells with 0.5% trypsin-EDTA solution and then, 3 ⁇ 5 ⁇ 10 4 cells/ml of cell suspension was prepared. Then, the cell suspension (180 ⁇ l/well) was added to 96-well plate, and the plate was incubated in an incubator of 37° C., 5% CO 2 for 24 hours.
  • the sample was dissolved in dimethylsulfoxide (DMSO) and diluted with the culture medium or tertiary distilled water to obtain required concentrations for experiment, and serially diluted to a final concentration of DMSO of 0.2% or less.
  • DMSO dimethylsulfoxide
  • To each well of 96-well plate were added 20 l of the serially diluted sample solutions, and then, the plate was incubated in an incubator of 37° C., 5% CO 2 for 48 hours. At the point of time to add the sample solution, T z (Time zero) plate was collected. Medium was removed from T z plate and from each plate after completing the incubation, and to the plates was added 10% trichloroacetic acid (TCA) (50 ⁇ l/well).
  • TCA trichloroacetic acid
  • the resulting plates were allowed to stand for 1 hour at 4° C. to immobilize the cells on the bottom of the plates. After completing the cell immobilization, the plates were washed 5 ⁇ 6 times with water to completely remove the remaining TCA solution, and the resulting plates were dried at room temperature to contain no moisture.
  • ED 50 value of the sample on tumor cells [50% effective dose (ng/ml): a concentration at which tumor cell growth is inhibited by 50%] was calculated as follows.
  • T z value was defined as OD value at the time of starting the incubation after adding the sample, C (control) value as OD value of the well not treated with the sample, and T (test) value as OD value of the well treated with the sample. From the values T z , C, and T, cytotoxicity of the agent was measured by the following formula:
  • WT fraction of 250 mg obtained in Example 1 was dissolved in. 10 ml of physiological saline to prepare an injectable solution.
  • SPX 3 fraction of 25 mg obtained in Example 2 was dissolved in 10 ml of Ringer's solution, sterilized and then, freeze-dried to prepare ready-to-use injectable dry powder. This powder would be re-constituted with distilled water for injection before use.
  • SB365 of 6.5 mg obtained in Example 3 was dissolved in 10 ml of Ringer's solution and sterilized to prepare an injectable solution.
  • Pulsatillae radix factions WT and SPX3, and SB365, hederagenin 3-O- ⁇ -L-rhamnopyranosyl((1 ⁇ 2)-[ ⁇ -D-glucopyranosyl(1 ⁇ 4)]- ⁇ -L-arabinopyranoside isolated from the fractions according to the present invention, not only have a high inhibition rate of tumor growth on solid tumor cells, but also can be conveniently used by dissolving in various solutions including physiological saline, Ringer's solution, or nutrient solution because it is readily soluble in water, and has low cytotoxicity enough to ameliorate side effects of previously developed anti-tumor agents. Therefore, it is anticipated to be very useful as a therapeutic agent for solid tumors.

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US10/620,709 2002-07-22 2003-07-15 Use of hederagenin 3-O-alpha-L-rhamnopyranosyl((1-2)-[Beta-D-glucopyranosyl(1-4)]-alpha-L-arabinopyranoside or an extract from pulsatillae radix containing the same as a therapeutic agent for solid tumors Abandoned US20040082528A1 (en)

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US11/107,665 US7682638B2 (en) 2002-07-22 2005-04-15 Use of hederagenin 3-O-α-L-rhamnopyranosyl(1-2)-[β-D-glucopyranosyl(1-4)]-α-L-arabinopyranoside or an extract from pulsatillae radix containing the same as a therapeutic agent for solid tumors
US12/709,907 US8075923B2 (en) 2002-07-22 2010-02-22 Use of hederagenin 3-O-α-L-rhamnopyranosyl(1→2)-(β-D-glucopyranosyl(1→4)-α-L-arabinopyranoside or an extract from pulsatillae radix containing the same as a therapeutic agent for solid tumors

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KR1020020043016A KR100568607B1 (ko) 2002-07-22 2002-07-22 헤데라게닌3-Ｏ-α-Ｌ-람노피라노실(1→2)-[β-Ｄ-글루코피라노실(1→4)]-α-Ｌ-아라비노피라노사이드 또는 그를 함유하는백두옹 추출물의 고형암 치료제로서의 용도
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CN102199184B (zh) * 2011-04-07 2013-03-06 江西本草天工科技有限责任公司 一种常春藤皂苷类衍生物、其盐的制备方法及其抗肿瘤的用途
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