PL3744834T3 - Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło - Google Patents

Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło

Info

Publication number
PL3744834T3
PL3744834T3 PL20169057.5T PL20169057T PL3744834T3 PL 3744834 T3 PL3744834 T3 PL 3744834T3 PL 20169057 T PL20169057 T PL 20169057T PL 3744834 T3 PL3744834 T3 PL 3744834T3
Authority
PL
Poland
Prior art keywords
light output
enhanced light
oplophorus luciferases
synthetic oplophorus
synthetic
Prior art date
Application number
PL20169057.5T
Other languages
English (en)
Inventor
Lance P. Encell
Keith V. Wood
Monika G. Wood
Mary P. Hall
Paul Otto
Gediminas Vidugiris
Kristopher Zimmerman
Original Assignee
Promega Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corporation filed Critical Promega Corporation
Publication of PL3744834T3 publication Critical patent/PL3744834T3/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PL20169057.5T 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło PL3744834T3 (pl)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US17483809P 2009-05-01 2009-05-01

Publications (1)

Publication Number Publication Date
PL3744834T3 true PL3744834T3 (pl) 2026-01-05

Family

ID=42358981

Family Applications (5)

Application Number Title Priority Date Filing Date
PL20169057.5T PL3744834T3 (pl) 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło
PL15186652T PL2990478T3 (pl) 2009-05-01 2010-05-03 Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła
PL18184699T PL3409764T3 (pl) 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło
PL17154777T PL3181687T3 (pl) 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplophorus emitującej bardziej intensywne światło
PL10717013T PL2456864T3 (pl) 2009-05-01 2010-05-03 Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła

Family Applications After (4)

Application Number Title Priority Date Filing Date
PL15186652T PL2990478T3 (pl) 2009-05-01 2010-05-03 Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła
PL18184699T PL3409764T3 (pl) 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło
PL17154777T PL3181687T3 (pl) 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplophorus emitującej bardziej intensywne światło
PL10717013T PL2456864T3 (pl) 2009-05-01 2010-05-03 Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła

Country Status (16)

Country Link
US (7) US8557970B2 (pl)
EP (5) EP2990478B1 (pl)
JP (2) JP6038649B2 (pl)
KR (1) KR101665352B1 (pl)
CN (2) CN102459579B (pl)
AU (1) AU2010242771B2 (pl)
BR (1) BRPI1010019B1 (pl)
CA (1) CA2758572A1 (pl)
DK (4) DK3409764T3 (pl)
ES (5) ES2795287T3 (pl)
IL (3) IL215697B (pl)
LT (1) LT3409764T (pl)
PL (5) PL3744834T3 (pl)
PT (4) PT3181687T (pl)
SG (3) SG10201601281WA (pl)
WO (1) WO2010127368A1 (pl)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL3744834T3 (pl) 2009-05-01 2026-01-05 Promega Corporation Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło
AU2011323455B2 (en) 2010-11-02 2017-07-27 Promega Corporation Coelenterazine derivatives and methods of using same
EP2635582B1 (en) * 2010-11-02 2016-01-27 Promega Corporation Novel coelenterazine substrates and methods of use
JP5896679B2 (ja) * 2011-03-15 2016-03-30 オリンパス株式会社 オオオバボタル由来ルシフェラーゼ
WO2013078244A1 (en) 2011-11-21 2013-05-30 Promega Corporation Carboxy x rhodamine analogs
EP3536808A1 (en) 2012-02-21 2019-09-11 Laboratory Corporation of America Holdings Methods and systems for detection of microorganisms
US10519483B2 (en) 2012-02-21 2019-12-31 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
JP2015530114A (ja) 2012-09-26 2015-10-15 プロメガ コーポレイションPromega Corporation リアルタイムモニタリング
US9551705B2 (en) 2012-12-12 2017-01-24 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
US10067149B2 (en) * 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
CN104871001B (zh) 2012-12-12 2018-09-11 普洛麦格公司 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合
JP6511720B2 (ja) 2013-02-28 2019-05-15 Jnc株式会社 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法
KR20230079494A (ko) 2013-03-15 2023-06-07 프로메가 코포레이션 구조적 보완에 의한 바이오발광성의 활성화
US10168323B2 (en) 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
JP6604938B2 (ja) 2013-03-15 2019-11-13 プロメガ コーポレイション タンパク質を官能基または固体表面に共有結合的に係留するための基質
JP2016517687A (ja) 2013-03-27 2016-06-20 サンプル テクノロジーズ,インコーポレイティド 組換えファージ及び細菌検出方法
JP6040846B2 (ja) 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
EP3011062A4 (en) 2013-06-19 2017-06-14 Sample6 Technologies Inc. Phage-based bacterial detection assay
JP6484987B2 (ja) 2013-10-21 2019-03-20 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
JP6442825B2 (ja) 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
EP3099691B1 (en) 2014-01-29 2019-11-20 Promega Corporation Pro-substrates for live cell applications
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
JP6256118B2 (ja) 2014-03-11 2018-01-10 Jnc株式会社 エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法
JP6265020B2 (ja) 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
EP3191600A1 (en) 2014-09-11 2017-07-19 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US11365402B2 (en) 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
EP3191601B1 (en) 2014-09-12 2022-11-02 Promega Corporation Internal protein tags
WO2016127100A1 (en) 2015-02-05 2016-08-11 Promega Corporation Luciferase-based thermal shift assays
JP6876002B2 (ja) 2015-06-05 2021-05-26 プロメガ コーポレイションPromega Corporation 機能的要素を共有結合により係留させるための細胞透過性、細胞適合性、かつ開裂可能であるリンカー
WO2016210294A1 (en) 2015-06-25 2016-12-29 Promega Corporation Thienopyrrole compounds and uses thereof as inhibitors of oplophorus-derived luciferases
EP3510035B1 (en) 2016-09-09 2021-04-07 Promega Corporation Dual protected pro-coelenterazine substrates
CA3039406A1 (en) 2016-09-19 2018-03-22 University Of Southern California Non-radioactive cytotoxicity assays
ES2963830T3 (es) 2016-10-20 2024-04-02 Harvard College Ensayos in vitro y basados en células para medir la actividad de las neurotoxinas botulínicas
EP3535270B3 (en) 2016-11-01 2024-05-29 Promega Corporation Coelenterazine analogues tethered to energy acceptors
JP7139330B2 (ja) 2016-12-01 2022-09-20 プロメガ コーポレイション 細胞不透過性セレンテラジンアナログ
JP7161475B2 (ja) 2016-12-28 2022-10-26 プロメガ コーポレイション 官能化nanoluc阻害剤
CN111315875B (zh) * 2017-10-25 2024-02-20 豪夫迈·罗氏有限公司 改进修饰的/突变的细菌萤光素酶
EP3720975A1 (en) 2017-12-08 2020-10-14 Centre National de la Recherche Scientifique - CNRS Bioluminescent screening test for detecting cell cytolysis
CN111886345B (zh) 2018-03-30 2023-09-15 英威达纺织(英国)有限公司 高氢气利用率和气体再循环
EP3775182A1 (en) * 2018-03-30 2021-02-17 INVISTA Textiles (U.K.) Limited Materials and methods for biosynthetic manufacture of pimelic acid and utilization of synthetic polypeptides
CN111836898B (zh) 2018-03-30 2024-02-23 英威达纺织(英国)有限公司 用于在连续有氧发酵中控制溶解氧浓度的方法
JP7419260B2 (ja) 2018-05-01 2024-01-22 プロメガ コーポレイション NanoLuc自殺基質としてのセレンテラジン化合物
US11999943B2 (en) 2018-05-02 2024-06-04 Inv Nylon Chemicals Americas, Llc Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-CoA-TCA balance in species of the genera ralstonia and cupriavidus and organisms related thereto
WO2019213118A1 (en) 2018-05-02 2019-11-07 Invista North America S.A.R.L. Methods for controlling pha biosynthesis in cupriavidus or ralstonia
JP7432529B2 (ja) 2018-05-30 2024-02-16 プロメガ コーポレイション 広域スペクトルキナーゼ結合剤
WO2019232384A1 (en) 2018-06-01 2019-12-05 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
KR20250029277A (ko) 2018-10-03 2025-03-04 프로메가 코포레이션 코엘렌테라진 및 이의 유사체 및 유도체를 안정화하기 위한 조성물 및 방법
EP3887830A2 (en) 2018-11-28 2021-10-06 Promega Corporation Reactive peptide labeling
EP3891503A2 (en) 2018-12-04 2021-10-13 Promega Corporation Broad spectrum gpcr binding agents
JP7584433B2 (ja) 2019-03-20 2024-11-15 プロメガ コーポレイション 光親和性プローブ
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
MX2021015900A (es) 2019-06-21 2022-02-03 Laboratory Corp America Holdings Metodos para producir bacteriofagos mutantes para la deteccion de listeria.
AU2020308448A1 (en) 2019-06-24 2022-02-03 Promega Corporation Modified polyamine polymers for delivery of biomolecules into cells
EP4022045A1 (en) 2019-08-26 2022-07-06 Laboratory Corporation of America Holdings Devices and methods for detecting microorganisms using recombinant reproduction-deficient indicator bacteriophage
EP4028540B1 (en) 2019-09-11 2025-01-29 Laboratory Corporation of America Holdings Methods and systems for the rapid detection of microorganisms using recombinant infectious agents to express an indicator subunit
EP4065699A1 (en) 2019-11-27 2022-10-05 Promega Corporation Multipartite luciferase peptides and polypeptides
WO2021119149A1 (en) 2019-12-10 2021-06-17 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
JP2023512014A (ja) 2020-01-28 2023-03-23 フリーライン セラピューティクス リミテッド ウイルスベクターに対する中和抗体力価を決定するための改善されたアッセイ
EP4153770A2 (en) 2020-05-22 2023-03-29 Promega Corporation Enhancement of kinase target engagement
JP2023540934A (ja) 2020-08-28 2023-09-27 プロメガ コーポレイション Rasタンパク質のターゲットエンゲージメントアッセイ
JPWO2022138892A1 (pl) 2020-12-25 2022-06-30
CN115161296B (zh) * 2021-04-01 2024-08-13 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
CN113025586B (zh) * 2021-04-06 2022-10-04 湖北省中医院 一种经改造的萤光素酶突变体蛋白、生物发光探针、探针组、制备方法和检测方法
WO2023215505A1 (en) 2022-05-04 2023-11-09 Promega Corporation Modified dehalogenase with extended surface loop regions
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
JP2025515176A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 光活性化可能な化合物及びその使用
US20240060059A1 (en) 2022-05-04 2024-02-22 Promega Corporation Circularly permuted dehalogenase variants
GB202213479D0 (en) 2022-09-14 2022-10-26 Ipsen Biopharm Ltd Cell-free clostridial neurotoxin assays
WO2024059832A1 (en) * 2022-09-15 2024-03-21 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for assessing kinase activity
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay
CN119307519B (zh) * 2024-07-22 2025-11-25 苏州剂泰医药科技有限公司 编码荧光素酶的mRNA及其应用
CN119709661B (zh) * 2024-12-30 2025-10-10 佰翱得(无锡)新药开发有限公司 一种荧光素酶OLuc突变体及其应用

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8911957D0 (en) 1989-05-24 1989-07-12 Interox Chemicals Ltd Peroxyacid manufacture
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
AU698424C (en) * 1994-01-03 2002-10-10 Promega Corporation Mutant luciferases
EP2860252A1 (en) * 1997-09-19 2015-04-15 Promega Corporation Thermostable luciferases and methods of production
JP3694181B2 (ja) * 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体dna及び変異型ルシフェラーゼの製造法
RU2251571C2 (ru) * 1999-10-26 2005-05-10 Дзе Секретэри Оф Стейт Фор Дефенс РЕКОМБИНАНТНАЯ ТЕРМОСТАБИЛЬНАЯ ЛЮЦИФЕРАЗА, СПОСОБ ЕЕ ПОЛУЧЕНИЯ, ИЗОЛИРОВАННАЯ НУКЛЕИНОВАЯ КИСЛОТА, ЭКСПРЕССИРУЮЩИЙ ВЕКТОР, НАБОР ДЛЯ ИСПОЛЬЗОВАНИЯ В БИОЛЮМИНЕСЦЕНТНОМ АНАЛИЗЕ, АНАЛИТИЧЕСКИЙ ТЕСТ ДЛЯ ОПРЕДЕЛЕНИЯ ПРИСУТСТВИЯ В ОБРАЗЦЕ СоА
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
EP1156103B1 (en) * 2000-04-26 2010-08-11 Chisso Corporation Oplophorus luciferase
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
EP2433871A3 (en) 2002-06-19 2012-07-11 Medical Instill Technologies, Inc. Sterile filling machine having needle filling station within e-beam chamber
AU2005230854A1 (en) * 2004-03-31 2005-10-20 Xencor, Inc BMP-7 variants with improved properties
JP3844082B2 (ja) * 2005-04-08 2006-11-08 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体dna及び耐熱性ホタルルシフェラーゼの製造法
JP4999341B2 (ja) * 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
EP2327768B1 (en) * 2006-04-03 2015-09-09 Promega Corporation Permuted and nonpermuted luciferase biosensors
WO2008118445A1 (en) 2007-03-26 2008-10-02 Promega Corporation Methods to quench light from optical reactions
WO2009061413A2 (en) 2007-11-05 2009-05-14 Promega Corporation Hybrid fusion reporter and uses thereof
ATE542903T1 (de) * 2008-05-19 2012-02-15 Promega Corp Luciferase-biosensoren für camp
WO2010119721A1 (ja) * 2009-04-17 2010-10-21 独立行政法人産業技術総合研究所 超高輝度で安定な人工生物発光酵素
PL3744834T3 (pl) 2009-05-01 2026-01-05 Promega Corporation Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło
EP2635582B1 (en) * 2010-11-02 2016-01-27 Promega Corporation Novel coelenterazine substrates and methods of use

Also Published As

Publication number Publication date
CN102459579A (zh) 2012-05-16
US20190185908A1 (en) 2019-06-20
US20140120548A1 (en) 2014-05-01
SG175180A1 (en) 2011-11-28
US20200248228A1 (en) 2020-08-06
BRPI1010019A2 (pt) 2015-08-25
IL215697A0 (en) 2012-01-31
IL265263A (en) 2019-05-30
CN108192950B (zh) 2021-09-03
PL3181687T3 (pl) 2018-11-30
US8557970B2 (en) 2013-10-15
AU2010242771B2 (en) 2015-01-22
DK2456864T3 (en) 2015-12-21
IL274145B (en) 2021-07-29
WO2010127368A1 (en) 2010-11-04
US9777311B2 (en) 2017-10-03
ES2629390T3 (es) 2017-08-09
ES2795287T3 (es) 2020-11-23
SG10201601281WA (en) 2016-03-30
EP3181687A1 (en) 2017-06-21
US11667950B2 (en) 2023-06-06
US10233485B2 (en) 2019-03-19
US20100281552A1 (en) 2010-11-04
JP6038649B2 (ja) 2016-12-07
DK2990478T3 (en) 2017-05-15
PT2990478T (pt) 2017-05-08
IL215697B (en) 2019-03-31
US11365436B2 (en) 2022-06-21
US20210032676A1 (en) 2021-02-04
LT3409764T (lt) 2020-06-10
CN108192950A (zh) 2018-06-22
PT3409764T (pt) 2020-05-29
AU2010242771A1 (en) 2011-11-03
CN102459579B (zh) 2018-02-09
EP3744834C0 (en) 2025-09-10
JP2016028599A (ja) 2016-03-03
PL2456864T3 (pl) 2016-04-29
IL265263B (en) 2020-05-31
CA2758572A1 (en) 2010-11-04
IL274145A (en) 2020-06-30
JP2012525819A (ja) 2012-10-25
US10844422B2 (en) 2020-11-24
EP3181687B1 (en) 2018-08-08
SG10201901453PA (en) 2019-03-28
US10633690B2 (en) 2020-04-28
US20220389434A1 (en) 2022-12-08
PT3181687T (pt) 2018-10-19
EP3409764B1 (en) 2020-04-15
EP2456864A1 (en) 2012-05-30
EP3744834A1 (en) 2020-12-02
EP2990478B1 (en) 2017-04-05
EP2456864B1 (en) 2015-11-11
PL2990478T3 (pl) 2017-08-31
JP6227615B2 (ja) 2017-11-08
ES2691539T3 (es) 2018-11-27
ES2559505T3 (es) 2016-02-12
BRPI1010019B1 (pt) 2019-02-05
PL3409764T3 (pl) 2020-07-13
KR101665352B1 (ko) 2016-10-12
EP2990478A1 (en) 2016-03-02
US20180051318A1 (en) 2018-02-22
DK3409764T3 (da) 2020-05-25
KR20120088548A (ko) 2012-08-08
ES3042791T3 (en) 2025-11-24
EP3744834B1 (en) 2025-09-10
PT2456864E (pt) 2016-01-20
DK3181687T3 (en) 2018-09-10
EP3409764A1 (en) 2018-12-05

Similar Documents

Publication Publication Date Title
IL274145B (en) Synthetic oplophorus luciferases that emit a lot of light
EP2334209A4 (en) FILE HAVING HAND FASTENING HAIR Weaving
PT2317881T (pt) Artigo para fumar com secção transparente
GB0801571D0 (en) Fairy light
GB0908027D0 (en) Spetrophotometer with no moving parts
IL219029A0 (en) Pyrazolopyrimidine derivatives
GB2463796B (en) Light fitting
TWM348594U (en) Transportation implement with double back
GB0911076D0 (en) Light fitting arrangement
GB0817940D0 (en) Colouring techniques
GB0914890D0 (en) Toothbrush with education function
TWM387049U (en) Bicycle light
ES1071336Y (es) Sillon provisto de bandeja amovible
HU0900259D0 (en) Light source with improved technical features
GB2457532B (en) Covering article with engagement means
GB2451715B (en) Synthetic fuels
PH32008000938S1 (en) Coaster with beads
PH32008000946S1 (en) Coaster with beads
GB0801128D0 (en) Light fitting
HU0800270D0 (en) Mobilphone with buttery
IL201962A0 (en) Plastic neon-like light source
HU3828U (en) Light source with led assembled in light tube armature
GB0800046D0 (en) Dual power supply with lamp
GB0819199D0 (en) Furniture with detachable arpro
GB0816919D0 (en) Recessed light fitting