PL2456864T3 - Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła - Google Patents

Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła

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Publication number
PL2456864T3
PL2456864T3 PL10717013T PL10717013T PL2456864T3 PL 2456864 T3 PL2456864 T3 PL 2456864T3 PL 10717013 T PL10717013 T PL 10717013T PL 10717013 T PL10717013 T PL 10717013T PL 2456864 T3 PL2456864 T3 PL 2456864T3
Authority
PL
Poland
Prior art keywords
light output
enhanced light
oplophorus luciferases
synthetic oplophorus
synthetic
Prior art date
Application number
PL10717013T
Other languages
English (en)
Inventor
Lance P Encell
Keith V Wood
Monika G Wood
Mary Hall
Paul Otto
Gediminas Vidugiris
Kristopher Zimmerman
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of PL2456864T3 publication Critical patent/PL2456864T3/pl

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PL10717013T 2009-05-01 2010-05-03 Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła PL2456864T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US17483809P 2009-05-01 2009-05-01
PCT/US2010/033449 WO2010127368A1 (en) 2009-05-01 2010-05-03 Synthetic oplophorus luciferases with enhanced light output
EP10717013.6A EP2456864B1 (en) 2009-05-01 2010-05-03 Synthetic oplophorus luciferases with enhanced light output

Publications (1)

Publication Number Publication Date
PL2456864T3 true PL2456864T3 (pl) 2016-04-29

Family

ID=42358981

Family Applications (4)

Application Number Title Priority Date Filing Date
PL15186652T PL2990478T3 (pl) 2009-05-01 2010-05-03 Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła
PL18184699T PL3409764T3 (pl) 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło
PL17154777T PL3181687T3 (pl) 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplophorus emitującej bardziej intensywne światło
PL10717013T PL2456864T3 (pl) 2009-05-01 2010-05-03 Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła

Family Applications Before (3)

Application Number Title Priority Date Filing Date
PL15186652T PL2990478T3 (pl) 2009-05-01 2010-05-03 Syntetyczna lucyferaza z oplophorus o wzmocnionej emisji światła
PL18184699T PL3409764T3 (pl) 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło
PL17154777T PL3181687T3 (pl) 2009-05-01 2010-05-03 Syntetyczne lucyferazy z krewetki oplophorus emitującej bardziej intensywne światło

Country Status (16)

Country Link
US (7) US8557970B2 (pl)
EP (5) EP2990478B1 (pl)
JP (2) JP6038649B2 (pl)
KR (1) KR101665352B1 (pl)
CN (2) CN102459579B (pl)
AU (1) AU2010242771B2 (pl)
BR (1) BRPI1010019B1 (pl)
CA (1) CA2758572A1 (pl)
DK (4) DK2990478T3 (pl)
ES (4) ES2691539T3 (pl)
IL (3) IL215697B (pl)
LT (1) LT3409764T (pl)
PL (4) PL2990478T3 (pl)
PT (4) PT3181687T (pl)
SG (3) SG175180A1 (pl)
WO (1) WO2010127368A1 (pl)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2990478T3 (en) 2009-05-01 2017-05-15 Promega Corp SYNTHETIC OPLOPHORUS LUCIFERASES WITH IMPROVED LIGHT EMISSION
US9487520B2 (en) 2010-11-02 2016-11-08 Promega Corporation Coelenterazine derivatives and methods of using same
WO2012061530A2 (en) 2010-11-02 2012-05-10 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
JP5896679B2 (ja) * 2011-03-15 2016-03-30 オリンパス株式会社 オオオバボタル由来ルシフェラーゼ
US9056885B2 (en) 2011-11-21 2015-06-16 Promega Corporation Carboxy X rhodamine analogs
US10519483B2 (en) 2012-02-21 2019-12-31 Laboratory Corporation Of America Holdings Methods and systems for rapid detection of microorganisms using infectious agents
WO2013126584A1 (en) 2012-02-21 2013-08-29 Laboratory Corporation Of America Holdings Methods and systems for detection of microorganisms
WO2014052653A1 (en) * 2012-09-26 2014-04-03 Promega Corporation Real-time monitoring
KR102292431B1 (ko) 2012-12-12 2021-08-20 프로메가 코포레이션 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식
CA2894432C (en) * 2012-12-12 2022-06-07 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
JP6511720B2 (ja) * 2013-02-28 2019-05-15 Jnc株式会社 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法
US10168323B2 (en) * 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
BR112015023394B8 (pt) 2013-03-15 2023-09-26 Promega Corp Sistema e complexo bioluminescente
WO2014151282A1 (en) 2013-03-15 2014-09-25 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
AU2014241191A1 (en) 2013-03-27 2015-10-22 Sample Technologies, Inc. Recombinant phage and bacterial detection methods
JP6040846B2 (ja) 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
US9828625B2 (en) 2013-06-19 2017-11-28 Phage Diagnostics, Inc. Phage-based bacterial detection assay
JP6484987B2 (ja) 2013-10-21 2019-03-20 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
JP6442825B2 (ja) 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
JP6703484B2 (ja) 2014-01-29 2020-06-03 プロメガ コーポレイションPromega Corporation 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションPromega Corporation 生細胞への適用のためのプロ基質
JP6256118B2 (ja) 2014-03-11 2018-01-10 Jnc株式会社 エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法
JP6265020B2 (ja) * 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
EP3191600A1 (en) 2014-09-11 2017-07-19 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
JP6814132B2 (ja) 2014-09-12 2021-01-13 プロメガ コーポレイションPromega Corporation 内部タンパク質タグ
US11365402B2 (en) 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
US10571471B2 (en) 2015-02-05 2020-02-25 Promega Corporation Luciferase-based thermal shift assays
EP3304078B1 (en) 2015-06-05 2022-08-31 Promega Corporation Cell-permeable, cell-compatible, and cleavable linkers for covalent tethering of functional elements
CN107922428B (zh) 2015-06-25 2021-02-05 普洛麦格公司 噻吩并吡咯化合物及其作为刺虾源性荧光素酶的抑制剂的用途
EP3510035B1 (en) 2016-09-09 2021-04-07 Promega Corporation Dual protected pro-coelenterazine substrates
WO2018053542A1 (en) 2016-09-19 2018-03-22 University Of Southern California Non-radioactive cytotoxicity assays
KR102556363B1 (ko) 2016-10-20 2023-07-18 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 보툴리눔 신경독소의 활성을 측정하기 위한 시험관내 및 세포 기반 검정
WO2018085367A1 (en) 2016-11-01 2018-05-11 Promega Corporation Coelenterazine analogues tethered to energy acceptors
DK3548492T3 (en) 2016-12-01 2023-08-07 Promega Corp Cell impermeable coelenterazine analogues
JP7161475B2 (ja) 2016-12-28 2022-10-26 プロメガ コーポレイション 官能化nanoluc阻害剤
US11788068B2 (en) 2017-10-25 2023-10-17 Roche Molecular Systems, Inc. Modified/mutant bacterial luciferases
WO2019110817A1 (en) 2017-12-08 2019-06-13 Centre National De La Recherche Scientifique (Cnrs) Bioluminescent screening test for detecting cell cytolysis
CN111936623B (zh) * 2018-03-30 2023-08-25 英威达纺织(英国)有限公司 用于生物合成制造庚二酸的材料与方法和合成多肽的利用
WO2019213119A1 (en) 2018-05-01 2019-11-07 Promega Corporation Coelenterazine compounds as nanoluc suicide substrates
US20190352674A1 (en) 2018-05-02 2019-11-21 Invista North America S.A.R.L. Materials and methods for controlling limitation conditions in product biosynthesis for non-phb generating species of the genera ralstonia or cupriavidus and organisms related thereto
US11442063B2 (en) 2018-05-30 2022-09-13 Promega Corporation Broad spectrum kinase binding agents
WO2019232384A1 (en) 2018-06-01 2019-12-05 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
KR20210072040A (ko) 2018-10-03 2021-06-16 프로메가 코포레이션 코엘렌테라진 및 이의 유사체 및 유도체를 안정화하기 위한 조성물 및 방법
JP2022509200A (ja) 2018-11-28 2022-01-20 プロメガ コーポレイション 反応性ペプチド標識付け
US20200200765A1 (en) 2018-12-04 2020-06-25 Promega Corporation Broad spectrum gpcr binding agents
WO2020191339A1 (en) 2019-03-20 2020-09-24 Promega Corporation Photoaffinity probes
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
EP3987015A1 (en) 2019-06-21 2022-04-27 Laboratory Corporation of America Holdings Methods for producing mutant bacteriophages for the detection of listeria
WO2020263825A1 (en) 2019-06-24 2020-12-30 Promega Corporation Modified polyamine polymers for delivery of biomolecules into cells
CN114729332A (zh) 2019-08-26 2022-07-08 美国控股实验室公司 用于使用重组繁殖缺陷性指示噬菌体检测微生物的装置和方法
MX2022003092A (es) 2019-09-11 2022-06-08 Laboratory Corp America Holdings Metodos y sistemas para la deteccion rapida de microorganismos utilizando agentes infecciosos recombinantes para expresar una subunidad indicadora.
US20210262941A1 (en) 2019-11-27 2021-08-26 Promega Corporation Multipartite luciferase peptides and polypeptides
WO2021119149A1 (en) 2019-12-10 2021-06-17 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
US20230093697A1 (en) 2020-01-28 2023-03-23 Freeline Therapeutics Limited Improved assay for determining neutralising antibody titre to a viral vector
EP4153770A2 (en) 2020-05-22 2023-03-29 Promega Corporation Enhancement of kinase target engagement
EP4204808A1 (en) 2020-08-28 2023-07-05 Promega Corporation Target engagement assay for ras proteins
EP4257978A1 (en) 2020-12-25 2023-10-11 Chugai Seiyaku Kabushiki Kaisha Method for screening for candidate molecule capable of forming complex in conjunction with plurality of target molecules
CN113025586B (zh) * 2021-04-06 2022-10-04 湖北省中医院 一种经改造的萤光素酶突变体蛋白、生物发光探针、探针组、制备方法和检测方法
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
US20240132859A1 (en) 2022-05-04 2024-04-25 Promega Corporation Modified dehalogenase with extended surface loop regions
WO2023215497A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
US20240060059A1 (en) 2022-05-04 2024-02-22 Promega Corporation Circularly permuted dehalogenase variants
GB202213479D0 (en) 2022-09-14 2022-10-26 Ipsen Biopharm Ltd Cell-free clostridial neurotoxin assays
WO2024059832A1 (en) * 2022-09-15 2024-03-21 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for assessing kinase activity

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8911957D0 (en) 1989-05-24 1989-07-12 Interox Chemicals Ltd Peroxyacid manufacture
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
AU698424C (en) * 1994-01-03 2002-10-10 Promega Corporation Mutant luciferases
JP4537573B2 (ja) * 1997-09-19 2010-09-01 プロメガ・コーポレイシヨン 熱安定ルシフェラーゼと生産方法
JP3694181B2 (ja) 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体dna及び変異型ルシフェラーゼの製造法
AU772485C (en) 1999-10-26 2004-11-18 Promega Corporation Novel enzyme
EP1156103B1 (en) * 2000-04-26 2010-08-11 Chisso Corporation Oplophorus luciferase
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
CA2489804C (en) 2002-06-19 2008-03-25 Medical Instill Technologies, Inc. Sterile filling machine having needle filling station within e-beam chamber
EP1730186A2 (en) * 2004-03-31 2006-12-13 Xencor, Inc. Bmp-7 variants with improved properties
JP3844082B2 (ja) * 2005-04-08 2006-11-08 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体dna及び耐熱性ホタルルシフェラーゼの製造法
JP4999341B2 (ja) * 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
EP2004813B1 (en) * 2006-04-03 2015-06-03 Promega Corporation Permuted and nonpermuted luciferase biosensors binding cyclic nucleotides
US20080248511A1 (en) * 2007-03-26 2008-10-09 Promega Corporation Methods to quench light from optical reactions
CN101849005A (zh) * 2007-11-05 2010-09-29 普罗梅加公司 杂合融合报道子及其应用
ATE542903T1 (de) * 2008-05-19 2012-02-15 Promega Corp Luciferase-biosensoren für camp
WO2010119721A1 (ja) * 2009-04-17 2010-10-21 独立行政法人産業技術総合研究所 超高輝度で安定な人工生物発光酵素
DK2990478T3 (en) 2009-05-01 2017-05-15 Promega Corp SYNTHETIC OPLOPHORUS LUCIFERASES WITH IMPROVED LIGHT EMISSION
WO2012061530A2 (en) * 2010-11-02 2012-05-10 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

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JP6038649B2 (ja) 2016-12-07
EP3409764B1 (en) 2020-04-15
US9777311B2 (en) 2017-10-03
SG175180A1 (en) 2011-11-28
IL265263A (en) 2019-05-30
US20180051318A1 (en) 2018-02-22
AU2010242771B2 (en) 2015-01-22
IL274145A (en) 2020-06-30
LT3409764T (lt) 2020-06-10
US20200248228A1 (en) 2020-08-06
EP3744834A1 (en) 2020-12-02
IL215697A0 (en) 2012-01-31
IL265263B (en) 2020-05-31
US10633690B2 (en) 2020-04-28
US20220389434A1 (en) 2022-12-08
ES2795287T3 (es) 2020-11-23
DK3181687T3 (en) 2018-09-10
EP2990478B1 (en) 2017-04-05
AU2010242771A1 (en) 2011-11-03
CN108192950A (zh) 2018-06-22
PT3409764T (pt) 2020-05-29
US10844422B2 (en) 2020-11-24
PL3181687T3 (pl) 2018-11-30
WO2010127368A1 (en) 2010-11-04
EP3409764A1 (en) 2018-12-05
US11365436B2 (en) 2022-06-21
IL274145B (en) 2021-07-29
US11667950B2 (en) 2023-06-06
EP3181687A1 (en) 2017-06-21
PT3181687T (pt) 2018-10-19
KR101665352B1 (ko) 2016-10-12
IL215697B (en) 2019-03-31
US20140120548A1 (en) 2014-05-01
PL2990478T3 (pl) 2017-08-31
CN108192950B (zh) 2021-09-03
JP6227615B2 (ja) 2017-11-08
PL3409764T3 (pl) 2020-07-13
EP2456864B1 (en) 2015-11-11
BRPI1010019A2 (pt) 2015-08-25
PT2990478T (pt) 2017-05-08
ES2691539T3 (es) 2018-11-27
US20190185908A1 (en) 2019-06-20
DK2990478T3 (en) 2017-05-15
US20100281552A1 (en) 2010-11-04
EP3181687B1 (en) 2018-08-08
CN102459579B (zh) 2018-02-09
US10233485B2 (en) 2019-03-19
CA2758572A1 (en) 2010-11-04
EP2990478A1 (en) 2016-03-02
ES2559505T3 (es) 2016-02-12
DK3409764T3 (da) 2020-05-25
JP2016028599A (ja) 2016-03-03
KR20120088548A (ko) 2012-08-08
US20210032676A1 (en) 2021-02-04
JP2012525819A (ja) 2012-10-25
CN102459579A (zh) 2012-05-16
SG10201601281WA (en) 2016-03-30
US8557970B2 (en) 2013-10-15
EP2456864A1 (en) 2012-05-30
PT2456864E (pt) 2016-01-20
SG10201901453PA (en) 2019-03-28
ES2629390T3 (es) 2017-08-09
DK2456864T3 (en) 2015-12-21
BRPI1010019B1 (pt) 2019-02-05

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