NO306684B1 - FremgangsmÕte for fremstilling av nitrilhydratase og anvendelse av nevnte nitrilhydratase og anvendelse av transformant inneholdende en rekombinant DNA som koder for et polypeptid med nitrilhydrataseaktivitet - Google Patents
FremgangsmÕte for fremstilling av nitrilhydratase og anvendelse av nevnte nitrilhydratase og anvendelse av transformant inneholdende en rekombinant DNA som koder for et polypeptid med nitrilhydrataseaktivitet Download PDFInfo
- Publication number
- NO306684B1 NO306684B1 NO910797A NO910797A NO306684B1 NO 306684 B1 NO306684 B1 NO 306684B1 NO 910797 A NO910797 A NO 910797A NO 910797 A NO910797 A NO 910797A NO 306684 B1 NO306684 B1 NO 306684B1
- Authority
- NO
- Norway
- Prior art keywords
- nitrile hydratase
- dna
- transformant
- subunit
- added
- Prior art date
Links
- 108010024026 Nitrile hydratase Proteins 0.000 title claims abstract description 59
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 13
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 13
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 13
- 230000000694 effects Effects 0.000 title claims abstract description 11
- 108020004511 Recombinant DNA Proteins 0.000 title claims abstract description 8
- 238000000034 method Methods 0.000 title claims description 14
- 238000002360 preparation method Methods 0.000 title claims description 5
- 108020004414 DNA Proteins 0.000 claims abstract description 49
- 239000012634 fragment Substances 0.000 claims abstract description 43
- 150000001408 amides Chemical class 0.000 claims abstract description 13
- 150000002825 nitriles Chemical class 0.000 claims abstract description 13
- 230000001580 bacterial effect Effects 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 6
- 239000013598 vector Substances 0.000 claims description 11
- 239000002773 nucleotide Substances 0.000 claims description 9
- 125000003729 nucleotide group Chemical group 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 8
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- 238000006703 hydration reaction Methods 0.000 claims description 4
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- 102000004190 Enzymes Human genes 0.000 abstract description 9
- 108090000790 Enzymes Proteins 0.000 abstract description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract 2
- 230000000887 hydrating effect Effects 0.000 abstract 2
- 239000000203 mixture Substances 0.000 description 34
- 239000013612 plasmid Substances 0.000 description 17
- 210000004027 cell Anatomy 0.000 description 14
- 241000187693 Rhodococcus rhodochrous Species 0.000 description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 150000001413 amino acids Chemical class 0.000 description 11
- 239000000523 sample Substances 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 9
- 239000000725 suspension Substances 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
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- KXDAEFPNCMNJSK-UHFFFAOYSA-N Benzamide Chemical compound NC(=O)C1=CC=CC=C1 KXDAEFPNCMNJSK-UHFFFAOYSA-N 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
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- 229960000723 ampicillin Drugs 0.000 description 6
- JFDZBHWFFUWGJE-UHFFFAOYSA-N benzonitrile Chemical compound N#CC1=CC=CC=C1 JFDZBHWFFUWGJE-UHFFFAOYSA-N 0.000 description 6
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- 238000002105 Southern blotting Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
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- 108020003215 DNA Probes Proteins 0.000 description 4
- 239000003298 DNA probe Substances 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
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- 229960005542 ethidium bromide Drugs 0.000 description 4
- -1 hydrates nitriles Chemical class 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 239000011541 reaction mixture Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
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- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 3
- 102000016943 Muramidase Human genes 0.000 description 3
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- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
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- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
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- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- BNBRIFIJRKJGEI-UHFFFAOYSA-N 2,6-difluorobenzonitrile Chemical compound FC1=CC=CC(F)=C1C#N BNBRIFIJRKJGEI-UHFFFAOYSA-N 0.000 description 1
- FFNVQNRYTPFDDP-UHFFFAOYSA-N 2-cyanopyridine Chemical class N#CC1=CC=CC=N1 FFNVQNRYTPFDDP-UHFFFAOYSA-N 0.000 description 1
- YXDXXGXWFJCXEB-UHFFFAOYSA-N 2-furonitrile Chemical compound N#CC1=CC=CO1 YXDXXGXWFJCXEB-UHFFFAOYSA-N 0.000 description 1
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- GUPXYSSGJWIURR-UHFFFAOYSA-N 3-octoxypropane-1,2-diol Chemical compound CCCCCCCCOCC(O)CO GUPXYSSGJWIURR-UHFFFAOYSA-N 0.000 description 1
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
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- 239000004471 Glycine Substances 0.000 description 1
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- 241001467578 Microbacterium Species 0.000 description 1
- 241000192041 Micrococcus Species 0.000 description 1
- 108010033272 Nitrilase Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
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- 239000001888 Peptone Substances 0.000 description 1
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- 239000008272 agar Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
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- 125000003118 aryl group Chemical group 0.000 description 1
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- PMSVVUSIPKHUMT-UHFFFAOYSA-N cyanopyrazine Chemical compound N#CC1=CN=CC=N1 PMSVVUSIPKHUMT-UHFFFAOYSA-N 0.000 description 1
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- 229920001220 nitrocellulos Polymers 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4807890 | 1990-02-28 | ||
| US08/028,463 US5731176A (en) | 1990-02-28 | 1993-03-09 | DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO910797D0 NO910797D0 (no) | 1991-02-27 |
| NO910797L NO910797L (no) | 1991-08-29 |
| NO306684B1 true NO306684B1 (no) | 1999-12-06 |
Family
ID=26388301
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO910797A NO306684B1 (no) | 1990-02-28 | 1991-02-27 | FremgangsmÕte for fremstilling av nitrilhydratase og anvendelse av nevnte nitrilhydratase og anvendelse av transformant inneholdende en rekombinant DNA som koder for et polypeptid med nitrilhydrataseaktivitet |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US5731176A (zh) |
| EP (1) | EP0445646B1 (zh) |
| JP (1) | JP3162091B2 (zh) |
| KR (1) | KR950002004B1 (zh) |
| CN (1) | CN1054639C (zh) |
| AT (1) | ATE138101T1 (zh) |
| AU (1) | AU627648B2 (zh) |
| CA (1) | CA2037291C (zh) |
| CZ (2) | CZ284048B6 (zh) |
| DE (1) | DE69119454T2 (zh) |
| DK (1) | DK0445646T3 (zh) |
| ES (1) | ES2089044T3 (zh) |
| FI (1) | FI102539B1 (zh) |
| MX (1) | MX193790B (zh) |
| NO (1) | NO306684B1 (zh) |
| PL (1) | PL166201B1 (zh) |
| RU (1) | RU2081173C1 (zh) |
| SK (1) | SK280199B6 (zh) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2907479B2 (ja) * | 1990-02-28 | 1999-06-21 | 輝彦 別府 | ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法 |
| EP0502476B1 (en) * | 1991-03-04 | 2001-07-18 | Mitsubishi Rayon Co., Ltd. | Hybrid plasmid vectors containing genes encoding nitrile degrading enzymes and methods of producing amides and acids |
| AU3692593A (en) * | 1992-04-28 | 1993-11-04 | Mitsubishi Kasei Corporation | Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene |
| JP3828590B2 (ja) * | 1994-10-03 | 2006-10-04 | 昌 清水 | ニトリルヒドラターゼ遺伝子発現のための調節遺伝子 |
| JP3154633B2 (ja) * | 1994-12-28 | 2001-04-09 | 三菱レイヨン株式会社 | ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子 |
| AU7514896A (en) * | 1995-10-06 | 1997-04-28 | E.I. Du Pont De Nemours And Company | Nucleic acid fragments encoding stereospecific nitrile hydratase and amidase enzymes and recombinant microorganisms expressing those enzymes useful for the production of chiral amides and acides |
| CN1101470C (zh) * | 1996-02-14 | 2003-02-12 | 三井东压化学株式会社 | 新型腈水合酶 |
| US5866379A (en) * | 1997-01-28 | 1999-02-02 | Novus International | Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha. |
| FR2770214B1 (fr) * | 1997-10-24 | 1999-12-31 | Rhodia Chimie Sa | Procede de preparation d'un benzamide halogene |
| JP2000050866A (ja) * | 1998-08-06 | 2000-02-22 | Rikagaku Kenkyusho | ニトリルヒドラターゼの生産方法 |
| JP4185607B2 (ja) | 1998-12-15 | 2008-11-26 | ダイセル化学工業株式会社 | 新規な微生物及びアミド化合物の製造方法 |
| CN1340101A (zh) * | 1999-10-26 | 2002-03-13 | 昭和电工株式会社 | 新型红球菌属细菌、源自于红球菌属细菌的腈水解酶基因、腈水合酶基因和酰胺酶基因,以及利用它们生产羧酸的方法 |
| RU2288270C2 (ru) | 2000-12-20 | 2006-11-27 | Диа-Нитрикс Ко., Лтд. | Способ производства амидного соединения с применением микробного катализатора |
| PT1352050E (pt) * | 2001-01-09 | 2009-03-24 | Lonza Ag | Processo microbiológico para a produção de amidas |
| JP2002325587A (ja) * | 2001-03-02 | 2002-11-12 | Daicel Chem Ind Ltd | ニトリルヒドラターゼ、およびアミドの製造方法 |
| JP2004194588A (ja) | 2002-12-19 | 2004-07-15 | Mitsui Chemicals Inc | 新規なニトリルヒドラターゼ |
| WO2004067738A2 (en) * | 2003-01-27 | 2004-08-12 | Degussa Ag | Nitrile hydratases from rhodococcus erythropolis and their application |
| SG148992A1 (en) | 2003-12-02 | 2009-01-29 | Ciba Spec Chem Water Treat Ltd | Strain of rhodococcus rhodochrous ncimb 41164 and its use as producer of nitrile hydratase |
| CN103146670B (zh) * | 2004-05-26 | 2014-11-26 | Dia-Nitrix株式会社 | 改良型腈水合酶 |
| JP2006187257A (ja) | 2005-01-07 | 2006-07-20 | Daiyanitorikkusu Kk | アミド化合物の製造方法およびアクリルアミド系ポリマー |
| EP1842907A1 (en) | 2006-04-07 | 2007-10-10 | B.R.A.I.N. Ag | A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity |
| WO2008004473A1 (fr) * | 2006-07-06 | 2008-01-10 | University Of Tsukuba | Nouveau complexe de protéines, procédé de maturation de nitrile hydratase à faible poids moléculaire de type au cobalt utilisant le complexe de protéines, nitrile hydratase correspondante et procédé l'utilisant |
| WO2008056827A1 (en) | 2006-11-09 | 2008-05-15 | Mitsubishi Rayon Co., Ltd. | Process for production of betaine |
| AU2012264058B2 (en) | 2011-05-31 | 2015-10-22 | Mitsubishi Chemical Corporation | Improved nitrile hydratase |
| US9193966B2 (en) | 2011-06-07 | 2015-11-24 | Mitsubishi Rayon Co., Ltd. | Nitrile hydratase |
| FR3002542B1 (fr) * | 2013-02-28 | 2016-01-22 | Servier Lab | Procede de synthese enzymatique de l'acide (7s) 3,4-dimethoxybicyclo[4.2.0]octa-1,3,5-triene 7-carboxylique et application a la synthese de l'ivabradine et de ses sels |
| KR101995103B1 (ko) * | 2014-06-06 | 2019-07-03 | 미쯔비시 케미컬 주식회사 | 개량형 니트릴 히드라타제 |
| EP3535386A4 (en) * | 2016-11-04 | 2020-04-15 | Georgia State University Research Foundation, Inc. | ENDOTOXIN-FREE ASPARAGINASE |
| CN116888269A (zh) | 2021-02-10 | 2023-10-13 | 三菱化学株式会社 | 用醛提高腈水合酶的反应性 |
| CN114934006A (zh) * | 2022-06-02 | 2022-08-23 | 无锡新晨宇生物工程有限公司 | 一种腈水合酶催化乙腈生成乙酰胺的应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD274631A5 (de) * | 1987-09-18 | 1989-12-27 | Kk | Verfahren zur biologischen herstellung von amiden |
| JP2840253B2 (ja) * | 1988-07-06 | 1998-12-24 | 輝彦 別府 | ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体によるニトリル類からアミド類の製造法 |
-
1991
- 1991-02-22 AU AU71293/91A patent/AU627648B2/en not_active Expired
- 1991-02-27 ES ES91102937T patent/ES2089044T3/es not_active Expired - Lifetime
- 1991-02-27 AT AT91102937T patent/ATE138101T1/de not_active IP Right Cessation
- 1991-02-27 EP EP91102937A patent/EP0445646B1/en not_active Expired - Lifetime
- 1991-02-27 JP JP03323691A patent/JP3162091B2/ja not_active Expired - Lifetime
- 1991-02-27 RU SU914894871A patent/RU2081173C1/ru active
- 1991-02-27 DE DE69119454T patent/DE69119454T2/de not_active Expired - Lifetime
- 1991-02-27 DK DK91102937.9T patent/DK0445646T3/da active
- 1991-02-27 FI FI910960A patent/FI102539B1/fi active
- 1991-02-27 MX MX9124722A patent/MX193790B/es unknown
- 1991-02-27 NO NO910797A patent/NO306684B1/no not_active IP Right Cessation
- 1991-02-28 CA CA002037291A patent/CA2037291C/en not_active Expired - Lifetime
- 1991-02-28 SK SK531-91A patent/SK280199B6/sk not_active IP Right Cessation
- 1991-02-28 KR KR1019910003258A patent/KR950002004B1/ko not_active IP Right Cessation
- 1991-02-28 CZ CS91531A patent/CZ284048B6/cs not_active IP Right Cessation
- 1991-02-28 CZ CZ943170A patent/CZ284017B6/cs not_active IP Right Cessation
- 1991-02-28 PL PL91289239A patent/PL166201B1/pl unknown
- 1991-02-28 CN CN91101323A patent/CN1054639C/zh not_active Expired - Lifetime
-
1993
- 1993-03-09 US US08/028,463 patent/US5731176A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CZ284017B6 (cs) | 1998-07-15 |
| CN1054639C (zh) | 2000-07-19 |
| JPH04211379A (ja) | 1992-08-03 |
| ATE138101T1 (de) | 1996-06-15 |
| DK0445646T3 (da) | 1996-09-30 |
| MX193790B (en) | 1999-10-19 |
| CS9100531A2 (en) | 1991-10-15 |
| KR910021473A (ko) | 1991-12-20 |
| RU2081173C1 (ru) | 1997-06-10 |
| NO910797D0 (no) | 1991-02-27 |
| SK280199B6 (sk) | 1999-09-10 |
| CZ284048B6 (cs) | 1998-07-15 |
| CZ317094A3 (cs) | 1998-05-13 |
| EP0445646A2 (en) | 1991-09-11 |
| KR950002004B1 (ko) | 1995-03-08 |
| CA2037291C (en) | 2001-07-24 |
| PL289239A1 (en) | 1992-03-23 |
| FI910960A (fi) | 1991-08-29 |
| AU7129391A (en) | 1991-09-12 |
| AU627648B2 (en) | 1992-08-27 |
| FI102539B (fi) | 1998-12-31 |
| CA2037291A1 (en) | 1991-08-29 |
| DE69119454T2 (de) | 1996-09-26 |
| CN1054616A (zh) | 1991-09-18 |
| ES2089044T3 (es) | 1996-10-01 |
| PL166201B1 (pl) | 1995-04-28 |
| JP3162091B2 (ja) | 2001-04-25 |
| NO910797L (no) | 1991-08-29 |
| EP0445646B1 (en) | 1996-05-15 |
| EP0445646A3 (en) | 1992-01-08 |
| DE69119454D1 (de) | 1996-06-20 |
| US5731176A (en) | 1998-03-24 |
| FI910960A0 (fi) | 1991-02-27 |
| FI102539B1 (fi) | 1998-12-31 |
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