KR910021473A - 니트릴히드라타제 활성을 갖는 폴리헵티드를 코팅하는 dna단편, 이것을 함유하는 형질 전환체 및 이 전환체를 사용하여 아미드류를 제조하는 방법 - Google Patents

니트릴히드라타제 활성을 갖는 폴리헵티드를 코팅하는 dna단편, 이것을 함유하는 형질 전환체 및 이 전환체를 사용하여 아미드류를 제조하는 방법 Download PDF

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KR910021473A
KR910021473A KR1019910003258A KR910003258A KR910021473A KR 910021473 A KR910021473 A KR 910021473A KR 1019910003258 A KR1019910003258 A KR 1019910003258A KR 910003258 A KR910003258 A KR 910003258A KR 910021473 A KR910021473 A KR 910021473A
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nitrile hydratase
sub unit
subunit
transformants
transformant
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KR1019910003258A
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KR950002004B1 (ko
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데루히꼬 베뿌
히데아끼 야마다
도오루 나가사와
스에하루 호리노우찌
마꼬또 니시야마
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데루히꼬 베뿌
이또 타이조
히데아끼 야마다
닛또오 가가꾸 고오교오 가부시끼가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

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  • Plant Pathology (AREA)
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Abstract

내용 없음

Description

니트릴히드라타제 활성을 갖는 폴리헵티드를 코팅하는 DNA단편, 이것을 함유하는 형질 전환체 및 이 전환체를 사용하여 아미드류를 제조하는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 로도코카스(Rhodococcus)로도크로우스(rhodochrous) J-1에 의해서 제조되는 2종류의 니트릴히드라타제의 α 및β서브유니트의 N-말단 아미노산 배열, 제2도는 DNA스포브로서 사용한 로도코카스 SP, N-774의 니트릴히드라타제유전자의 DNA배열, 제3도는 제조합 플라스미드, PNHJ10및 PNHJ20L의 제한 효소지도.

Claims (9)

  1. 하기와 같은-서브 유니트와-서브 유니트의 아미노산 배열을 갖고 니트릴히드라타제활성을 갖는 폴리펩티드를 코딩하는단편-서브 유니트.
    -서브 유니트
  2. 하기와 같은-서브 유니트와-서브 유니트의 아미노산배열을 갖고 니트릴히드라타제활성을 갖는 폴리펩티드를 코딩하는-단편-서브 유니트
    -서브 유니트
  3. 제1항에 있어서,-서브 유니트와-서브 유니트의 다음DNA배열을 갖는- 단편-서브 유니트 DNA배열 :
    -서브 유니트의 DNA배열 :
  4. 제2항에 있어서,-서브 유니트와-서브 유니트의 다음 DNA배열을 갖는-단편-서브 유니트의 DNA배열
    -서브 유니트의 DNA배열 :
  5. 제1∼4항 기재의또는를 백터중에 포함하는 재조합체 DNA.
  6. 제5항 기재의 재조합체 DNA로 형질 전환된 형질전환체.
  7. 제6항 기재의 형질변환체를 배양하는 공정과, 그 배양물로부터 니트릴히드라타제를 회수하는 공정을 포함하는 니트릴히드라타제를 제조하는 방법.
  8. 제6항 기재의 형질전환체의 배양물로 부터 얻어지는 니트릴히드라타제를 사용하여 니트릴류를 수화시키는 공정을 포함하는 아미드류를 제조하는 방법.
  9. 제6항 기재의 형질전환체를 배양하는 공정과 얻어진 배양물로 분리균제, 균제처리물 및 그들의 공정화물을 사용하여 니트릴류를 수화하여 아미드류화는 공정을 포함하는 아미드류를 제조하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910003258A 1990-02-28 1991-02-28 니트릴하이드라타제활성을갖는폴리펩티드를코딩하는dna단편,이것을함유하는형질전환체및이전환체를사용하여아미드류를제조하는방법 KR950002004B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2-48078 1990-02-28
JP02-48078 1990-02-28
JP4807890 1990-02-28
US08/028,463 US5731176A (en) 1990-02-28 1993-03-09 DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant

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KR910021473A true KR910021473A (ko) 1991-12-20
KR950002004B1 KR950002004B1 (ko) 1995-03-08

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KR1019910003258A KR950002004B1 (ko) 1990-02-28 1991-02-28 니트릴하이드라타제활성을갖는폴리펩티드를코딩하는dna단편,이것을함유하는형질전환체및이전환체를사용하여아미드류를제조하는방법

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US (1) US5731176A (ko)
EP (1) EP0445646B1 (ko)
JP (1) JP3162091B2 (ko)
KR (1) KR950002004B1 (ko)
CN (1) CN1054639C (ko)
AT (1) ATE138101T1 (ko)
AU (1) AU627648B2 (ko)
CA (1) CA2037291C (ko)
CZ (2) CZ284048B6 (ko)
DE (1) DE69119454T2 (ko)
DK (1) DK0445646T3 (ko)
ES (1) ES2089044T3 (ko)
FI (1) FI102539B1 (ko)
MX (1) MX193790B (ko)
NO (1) NO306684B1 (ko)
PL (1) PL166201B1 (ko)
RU (1) RU2081173C1 (ko)
SK (1) SK280199B6 (ko)

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EP0502476B1 (en) * 1991-03-04 2001-07-18 Mitsubishi Rayon Co., Ltd. Hybrid plasmid vectors containing genes encoding nitrile degrading enzymes and methods of producing amides and acids
AU3692593A (en) * 1992-04-28 1993-11-04 Mitsubishi Kasei Corporation Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene
JP3828590B2 (ja) * 1994-10-03 2006-10-04 昌 清水 ニトリルヒドラターゼ遺伝子発現のための調節遺伝子
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CN1101470C (zh) * 1996-02-14 2003-02-12 三井东压化学株式会社 新型腈水合酶
US5866379A (en) * 1997-01-28 1999-02-02 Novus International Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha.
FR2770214B1 (fr) * 1997-10-24 1999-12-31 Rhodia Chimie Sa Procede de preparation d'un benzamide halogene
JP2000050866A (ja) * 1998-08-06 2000-02-22 Rikagaku Kenkyusho ニトリルヒドラターゼの生産方法
JP4185607B2 (ja) 1998-12-15 2008-11-26 ダイセル化学工業株式会社 新規な微生物及びアミド化合物の製造方法
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WO2008004473A1 (fr) * 2006-07-06 2008-01-10 University Of Tsukuba Nouveau complexe de protéines, procédé de maturation de nitrile hydratase à faible poids moléculaire de type au cobalt utilisant le complexe de protéines, nitrile hydratase correspondante et procédé l'utilisant
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KR101995103B1 (ko) * 2014-06-06 2019-07-03 미쯔비시 케미컬 주식회사 개량형 니트릴 히드라타제
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JP2840253B2 (ja) * 1988-07-06 1998-12-24 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体によるニトリル類からアミド類の製造法

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CZ284017B6 (cs) 1998-07-15
CN1054639C (zh) 2000-07-19
JPH04211379A (ja) 1992-08-03
ATE138101T1 (de) 1996-06-15
DK0445646T3 (da) 1996-09-30
MX193790B (en) 1999-10-19
CS9100531A2 (en) 1991-10-15
RU2081173C1 (ru) 1997-06-10
NO910797D0 (no) 1991-02-27
SK280199B6 (sk) 1999-09-10
CZ284048B6 (cs) 1998-07-15
CZ317094A3 (cs) 1998-05-13
NO306684B1 (no) 1999-12-06
EP0445646A2 (en) 1991-09-11
KR950002004B1 (ko) 1995-03-08
CA2037291C (en) 2001-07-24
PL289239A1 (en) 1992-03-23
FI910960A (fi) 1991-08-29
AU7129391A (en) 1991-09-12
AU627648B2 (en) 1992-08-27
FI102539B (fi) 1998-12-31
CA2037291A1 (en) 1991-08-29
DE69119454T2 (de) 1996-09-26
CN1054616A (zh) 1991-09-18
ES2089044T3 (es) 1996-10-01
PL166201B1 (pl) 1995-04-28
JP3162091B2 (ja) 2001-04-25
NO910797L (no) 1991-08-29
EP0445646B1 (en) 1996-05-15
EP0445646A3 (en) 1992-01-08
DE69119454D1 (de) 1996-06-20
US5731176A (en) 1998-03-24
FI910960A0 (fi) 1991-02-27
FI102539B1 (fi) 1998-12-31

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