FI910960A - Dna-fragment, som kodar en polypeptid med nitrilhydratasaktivitet, transformant innehaollande genen och foerfarande foer framstaellning av amiderar med hjaelp av transformanten. - Google Patents

Dna-fragment, som kodar en polypeptid med nitrilhydratasaktivitet, transformant innehaollande genen och foerfarande foer framstaellning av amiderar med hjaelp av transformanten. Download PDF

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Publication number
FI910960A
FI910960A FI910960A FI910960A FI910960A FI 910960 A FI910960 A FI 910960A FI 910960 A FI910960 A FI 910960A FI 910960 A FI910960 A FI 910960A FI 910960 A FI910960 A FI 910960A
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FI
Finland
Prior art keywords
med
transformant
nitrilhydratasaktivitet
amiderar
transformanten
Prior art date
Application number
FI910960A
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English (en)
Other versions
FI102539B1 (fi
FI910960A0 (fi
FI102539B (fi
Inventor
Teruhiko Beppu
Hideaki Yamada
Toru Nagasawa
Sueharu Horinouchi
Makoto Nishiyama
Original Assignee
Nitto Chemical Industry Co Ltd
Teruhiko Beppu
Hideaki Yamada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nitto Chemical Industry Co Ltd, Teruhiko Beppu, Hideaki Yamada filed Critical Nitto Chemical Industry Co Ltd
Publication of FI910960A0 publication Critical patent/FI910960A0/fi
Publication of FI910960A publication Critical patent/FI910960A/fi
Application granted granted Critical
Publication of FI102539B1 publication Critical patent/FI102539B1/fi
Publication of FI102539B publication Critical patent/FI102539B/fi

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
FI910960A 1990-02-28 1991-02-27 Nitriilihydrataasiaktiivisuutta omaavaa polypeptidiä koodaava DNA-frag mentti, geenin sisältävä transformantti ja menetelmä amidien tuottamis eksi transformanttia käyttäen FI102539B (fi)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP4807890 1990-02-28
JP4807890 1990-02-28
US08/028,463 US5731176A (en) 1990-02-28 1993-03-09 DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant
US2846393 1993-03-09

Publications (4)

Publication Number Publication Date
FI910960A0 FI910960A0 (fi) 1991-02-27
FI910960A true FI910960A (fi) 1991-08-29
FI102539B1 FI102539B1 (fi) 1998-12-31
FI102539B FI102539B (fi) 1998-12-31

Family

ID=26388301

Family Applications (1)

Application Number Title Priority Date Filing Date
FI910960A FI102539B (fi) 1990-02-28 1991-02-27 Nitriilihydrataasiaktiivisuutta omaavaa polypeptidiä koodaava DNA-frag mentti, geenin sisältävä transformantti ja menetelmä amidien tuottamis eksi transformanttia käyttäen

Country Status (18)

Country Link
US (1) US5731176A (fi)
EP (1) EP0445646B1 (fi)
JP (1) JP3162091B2 (fi)
KR (1) KR950002004B1 (fi)
CN (1) CN1054639C (fi)
AT (1) ATE138101T1 (fi)
AU (1) AU627648B2 (fi)
CA (1) CA2037291C (fi)
CZ (2) CZ284048B6 (fi)
DE (1) DE69119454T2 (fi)
DK (1) DK0445646T3 (fi)
ES (1) ES2089044T3 (fi)
FI (1) FI102539B (fi)
MX (1) MX193790B (fi)
NO (1) NO306684B1 (fi)
PL (1) PL166201B1 (fi)
RU (1) RU2081173C1 (fi)
SK (1) SK280199B6 (fi)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2907479B2 (ja) * 1990-02-28 1999-06-21 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法
EP0502476B1 (en) * 1991-03-04 2001-07-18 Mitsubishi Rayon Co., Ltd. Hybrid plasmid vectors containing genes encoding nitrile degrading enzymes and methods of producing amides and acids
AU3692593A (en) * 1992-04-28 1993-11-04 Mitsubishi Kasei Corporation Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene
JP3828590B2 (ja) * 1994-10-03 2006-10-04 昌 清水 ニトリルヒドラターゼ遺伝子発現のための調節遺伝子
JP3154633B2 (ja) * 1994-12-28 2001-04-09 三菱レイヨン株式会社 ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子
WO1997012964A2 (en) * 1995-10-06 1997-04-10 E.I. Du Pont De Nemours And Company Nucleic acid fragments encoding stereospecific nitrile hydratase and amidase enzymes and recombinant microorganisms expressing those enzymes useful for the production of chiral amides and acides
EP0790310B1 (en) * 1996-02-14 2008-10-08 Mitsui Chemicals, Inc. Nitrile hydratase, derivatives thereof and methods of producing compounds
US5866379A (en) * 1997-01-28 1999-02-02 Novus International Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha.
FR2770214B1 (fr) * 1997-10-24 1999-12-31 Rhodia Chimie Sa Procede de preparation d'un benzamide halogene
JP2000050866A (ja) * 1998-08-06 2000-02-22 Rikagaku Kenkyusho ニトリルヒドラターゼの生産方法
JP4185607B2 (ja) 1998-12-15 2008-11-26 ダイセル化学工業株式会社 新規な微生物及びアミド化合物の製造方法
WO2001030994A1 (fr) * 1999-10-26 2001-05-03 Showa Denko K.K. Nouveau rhodocoque, gene de la nitrilase, de la nitrilehydratase et de l'amidase, provenant du rhodocoque, et procede de production d'acides carboxyliques a l'aide de ceux-ci
JP4708677B2 (ja) 2000-12-20 2011-06-22 ダイヤニトリックス株式会社 微生物触媒を用いたアミド化合物の製造方法
WO2002055670A1 (de) * 2001-01-09 2002-07-18 Lonza Ag Mikrobiologisches verfahren zur herstellung von amiden
JP2002325587A (ja) 2001-03-02 2002-11-12 Daicel Chem Ind Ltd ニトリルヒドラターゼ、およびアミドの製造方法
JP2004194588A (ja) 2002-12-19 2004-07-15 Mitsui Chemicals Inc 新規なニトリルヒドラターゼ
WO2004067738A2 (en) * 2003-01-27 2004-08-12 Degussa Ag Nitrile hydratases from rhodococcus erythropolis and their application
AU2004295409B2 (en) 2003-12-02 2010-07-29 Ciba Specialty Chemicals Water Treatments Limited Strain of Rhodococcus rhodochrous NCIMB 41164 and its use as producer of nitrile hydratase
CN102604921B (zh) 2004-05-26 2014-12-03 Dia-Nitrix株式会社 改良型腈水合酶
JP2006187257A (ja) 2005-01-07 2006-07-20 Daiyanitorikkusu Kk アミド化合物の製造方法およびアクリルアミド系ポリマー
EP1842907A1 (en) 2006-04-07 2007-10-10 B.R.A.I.N. Ag A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity
JP5120852B2 (ja) * 2006-07-06 2013-01-16 国立大学法人 筑波大学 新規タンパク質複合体、該タンパク質複合体を用いたコバルト型低分子量ニトリルヒドラターゼ成熟化方法、成熟化コバルト型低分子量ニトリルヒドラターゼ、及び該ニトリルヒドラターゼを用いた方法
WO2008056827A1 (en) 2006-11-09 2008-05-15 Mitsubishi Rayon Co., Ltd. Process for production of betaine
WO2012164933A1 (ja) 2011-05-31 2012-12-06 ダイヤニトリックス株式会社 改良型ニトリルヒドラターゼ
CN103635574B (zh) 2011-06-07 2016-04-13 三菱丽阳株式会社 改良型腈水合酶
FR3002542B1 (fr) * 2013-02-28 2016-01-22 Servier Lab Procede de synthese enzymatique de l'acide (7s) 3,4-dimethoxybicyclo[4.2.0]octa-1,3,5-triene 7-carboxylique et application a la synthese de l'ivabradine et de ses sels
WO2015186298A1 (ja) * 2014-06-06 2015-12-10 三菱レイヨン株式会社 改良型ニトリルヒドラターゼ
WO2018085493A1 (en) * 2016-11-04 2018-05-11 Georgia State University Research Foundation, Inc. Endotoxin free asparaginase
EP4279600A1 (en) 2021-02-10 2023-11-22 Mitsubishi Chemical Corporation Improved nitrile hydratase reactivity using aldehyde
CN114934006A (zh) * 2022-06-02 2022-08-23 无锡新晨宇生物工程有限公司 一种腈水合酶催化乙腈生成乙酰胺的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD274631A5 (de) * 1987-09-18 1989-12-27 Kk Verfahren zur biologischen herstellung von amiden
JP2840253B2 (ja) * 1988-07-06 1998-12-24 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体によるニトリル類からアミド類の製造法

Also Published As

Publication number Publication date
CZ284017B6 (cs) 1998-07-15
FI102539B1 (fi) 1998-12-31
ES2089044T3 (es) 1996-10-01
US5731176A (en) 1998-03-24
DE69119454T2 (de) 1996-09-26
EP0445646B1 (en) 1996-05-15
DK0445646T3 (da) 1996-09-30
EP0445646A3 (en) 1992-01-08
CS9100531A2 (en) 1991-10-15
JP3162091B2 (ja) 2001-04-25
JPH04211379A (ja) 1992-08-03
CA2037291A1 (en) 1991-08-29
EP0445646A2 (en) 1991-09-11
PL289239A1 (en) 1992-03-23
NO910797D0 (no) 1991-02-27
KR910021473A (ko) 1991-12-20
FI910960A0 (fi) 1991-02-27
SK280199B6 (sk) 1999-09-10
CN1054616A (zh) 1991-09-18
NO306684B1 (no) 1999-12-06
MX193790B (en) 1999-10-19
PL166201B1 (pl) 1995-04-28
ATE138101T1 (de) 1996-06-15
AU7129391A (en) 1991-09-12
CA2037291C (en) 2001-07-24
FI102539B (fi) 1998-12-31
DE69119454D1 (de) 1996-06-20
AU627648B2 (en) 1992-08-27
CN1054639C (zh) 2000-07-19
RU2081173C1 (ru) 1997-06-10
NO910797L (no) 1991-08-29
CZ284048B6 (cs) 1998-07-15
KR950002004B1 (ko) 1995-03-08
CZ317094A3 (cs) 1998-05-13

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