KR920700287A - 신규의 프로테아제 억제물질 및 그것의 제조방법 - Google Patents

신규의 프로테아제 억제물질 및 그것의 제조방법

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Publication number
KR920700287A
KR920700287A KR1019900702307A KR900702307A KR920700287A KR 920700287 A KR920700287 A KR 920700287A KR 1019900702307 A KR1019900702307 A KR 1019900702307A KR 900702307 A KR900702307 A KR 900702307A KR 920700287 A KR920700287 A KR 920700287A
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KR
South Korea
Prior art keywords
gly
ala
cys
lys
pro
Prior art date
Application number
KR1019900702307A
Other languages
English (en)
Inventor
요시마사 이케
노부히코 카투누마
Original Assignee
사와무라 하루오
미츠이 도아츠 가가쿠 가부시기가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 사와무라 하루오, 미츠이 도아츠 가가쿠 가부시기가이샤 filed Critical 사와무라 하루오
Publication of KR920700287A publication Critical patent/KR920700287A/ko

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8135Kazal type inhibitors, e.g. pancreatic secretory inhibitor, ovomucoid
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

신규의 프로테아제 억제물질 및 그것의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 메티오닌 트립스타틴 유전자를 6개 단편으로 절단한 DNA 서열을 도시하며;
제2도는 6개의 합성 단편물의 DNA 서열을 도시하며;
제3도는 메티오닌 트립스타틴 합성 유전자를 수득하기 위한 단편물의 연결반응을 도시함.

Claims (7)

  1. 하기의 아미노산 서열을 코오딩한 메티오닌 트리스타틴.
    Met Ile Ala Ala Cys Asn Leu Pro Ile Val Gln Gly Pro Cys Arg Ala Phe Ala Glu Leu Leu Ala Phe Asp Ala Ala Gln Gly Lys Cys Ile Gln Phe Ile Tyr Gly Gly Cys Lys Gly Asn Asn Asn Lys Gly Tyr Ser Glu Pro Lys Cys Lys Trp Tyr Cys Gly Val Pro Gly Asp Gly Tyr
  2. 하기의 염기 서열을 갖는, 메티오닌 트립스타틴을 코오딩한 서열.
  3. N1, N2및 N3가 C 또는 T를 나타내며 하기의 서열을 갖는 메티오닌 트립스타틴을 코오딩한 DNA 서열.
  4. 제2항 또는 제3항 DNA를 형질발현 벡터에 삽입한 재조합 플라즈미드.
  5. 제4항의 재조합 플라즈미드에 의해 형질전환된 미생물.
  6. 제4항의 미생물을 배양하는 것을 특징으로 하는 메티오닌 트립스타틴 제조방법.
  7. 하기의 아미노산 서열을 포함하는 프로테아제 억제제 메티오닌 트립스타틴.
    Met Ile Ala Ala Cys Asn Leu Pro Ile Val Gln Gly Pro Cys Arg Ala Phe Ala Glu Leu Leu Ala Phe Asp Ala Ala Gln Gly Lys Cys Ile Gln Phe Ile Tyr Gly Gly Cys Lys Gly Asn Asn Asn Lys Gly Tyr Ser Glu Pro Lys Cys Lys Trp Tyr Cys Gly Val Pro Gly Asp Gly Tyr
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900702307A 1989-02-22 1990-02-22 신규의 프로테아제 억제물질 및 그것의 제조방법 KR920700287A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP4017589 1989-02-22
JP1-40175 1989-02-22
PCT/JP1990/000216 WO1990010066A1 (fr) 1989-02-22 1990-02-22 Nouvel inhibiteur de protease et production de ce compose

Publications (1)

Publication Number Publication Date
KR920700287A true KR920700287A (ko) 1992-02-19

Family

ID=12573438

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900702307A KR920700287A (ko) 1989-02-22 1990-02-22 신규의 프로테아제 억제물질 및 그것의 제조방법

Country Status (4)

Country Link
EP (1) EP0426860A4 (ko)
KR (1) KR920700287A (ko)
CA (1) CA2025537A1 (ko)
WO (1) WO1990010066A1 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW492975B (en) * 1993-07-26 2002-07-01 Novartis Ag Tryptase inhibitor
US5633227A (en) * 1994-09-12 1997-05-27 Miles, Inc. Secretory leukocyte protease inhibitor as an inhibitor of tryptase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59125895A (ja) * 1983-01-07 1984-07-20 Rikagaku Kenkyusho ヒト生長ホルモン遺伝子の合成法
GB2188933A (en) * 1986-04-10 1987-10-14 Bayer Ag Expression vectors for production of polypeptides, method for enhanced expression of polypeptides, hosts containing the expression vectors, products manufactured thereby

Also Published As

Publication number Publication date
EP0426860A4 (en) 1991-10-02
EP0426860A1 (en) 1991-05-15
CA2025537A1 (en) 1990-08-23
WO1990010066A1 (fr) 1990-09-07

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