KR850004791A - 프로테아제 억제제의 제조방법 - Google Patents
프로테아제 억제제의 제조방법 Download PDFInfo
- Publication number
- KR850004791A KR850004791A KR1019840007283A KR840007283A KR850004791A KR 850004791 A KR850004791 A KR 850004791A KR 1019840007283 A KR1019840007283 A KR 1019840007283A KR 840007283 A KR840007283 A KR 840007283A KR 850004791 A KR850004791 A KR 850004791A
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- KR
- South Korea
- Prior art keywords
- compound
- xiv
- radical
- iglin
- general formula
- Prior art date
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- 238000000034 method Methods 0.000 title claims 11
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 title 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims 16
- 108090000765 processed proteins & peptides Proteins 0.000 claims 7
- 150000003839 salts Chemical class 0.000 claims 5
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 claims 3
- 125000003277 amino group Chemical group 0.000 claims 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 2
- PPQRSMGDOHLTBE-UWVGGRQHSA-N Ser-Phe Chemical compound OC[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 PPQRSMGDOHLTBE-UWVGGRQHSA-N 0.000 claims 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- VLDVBZICYBVQHB-IUCAKERBSA-N His-Val Chemical compound CC(C)[C@@H](C([O-])=O)NC(=O)[C@@H]([NH3+])CC1=CN=CN1 VLDVBZICYBVQHB-IUCAKERBSA-N 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- TUCNEACPLKLKNU-UHFFFAOYSA-N acetyl Chemical compound C[C]=O TUCNEACPLKLKNU-UHFFFAOYSA-N 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000013613 expression plasmid Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 239000012634 fragment Substances 0.000 description 4
- 101150111062 C gene Proteins 0.000 description 2
- 239000013599 cloning vector Substances 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 101150076489 B gene Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/38—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against protease inhibitors of peptide structure
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Animal Behavior & Ethology (AREA)
- General Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Rheumatology (AREA)
- Pain & Pain Management (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 이글린(Eglin) C유전자의 F1(C) 단편 및 F2단편의 합성 도식이다.
제2도는 이글린 C유전자의 F1(C)단편에 대한 클로닝 벡터, 플라즈미드 pML 87의 제조과정이다.
제3도는 이글린 C 또는 이글린 B 유전자의 F2단편을 위한 클로닝 벡터, 플라즈미드 pML 136의 제조과정이다.
Claims (10)
- 발현 조절 서열에 의해서 조절된 이글린-코드화 DNA서열을 함유하는 발현 플라즈미드를 사용하여 형질전환시킨 숙주를 탄소 및 질소동화원을 함유하는 액체영양 배지내에서 배양하고, 숙주 세포로부터 생성물을 유리시키고 그것을 단리하거나, r이 0이고 N-말단의 아미노산이 N-아세틸화된 일반식(ⅩⅣ)의 화합물을 제조하기 위해서는 일반식(ⅩⅣ)의 화합물을 유리 N-말단의 아미노기로 아세틸화하고, 필요하다면 수득할 수 있는 일반식(ⅩⅣ)의 이글린 화합물을 일반식(ⅩⅣ)의 다른 이글린 화합물로 전환시키고, 필요하다면, 이 과정에서 수득한 일반식(ⅩⅣ)의 화합물의 혼합물을 각각의 성분으로 분리하고/하거나, 필요하다면, 생성된 염을 유리 폴리펩티드로 전환시키고 생성된 폴리펩티드를 그의 염으로 전환시킴을 특징으로 하여 일반식(ⅩⅣ)의 이글린 화합물 및 그의 염을 제조하는 방법.상기식에서, B는 직접 결합 또는 천연의 이글린의 N-말단으로부터 1 내지 10개의 아미노산 단위를 함유하는 펩티드라디칼, 예를들어, Serphe, LeuLysSerphe, SerGluLeuLysSerphe, PheGlySerGluLeuLysSerphe 및 ThrGlupheGlySerGluLeuLysSerphe으로부터 선정된 라디칼이며, B'는 펩티드 라디칼이아 니거나 천연의 이글린의 C-말단으로부터 1내지 6개의 아미노산 단위를 함유하는 펩티드라디칼, 예를들어 Hisval, HisvalproHis 또는 HisvalproHisvalGly으로부터 선정된 라디칼이며, W는 Try 또는 His이고, r은 0 또는 1이며, r이 0일때 N-말단 아미노산은 유리형이거나 N-아세틸화된 것이다.
- 제1항에 있어서 B가 LeuLysSerphe, SerGluLeuLysSerphe 및 ThrGlupheGlySerGluLeuLysSerphe으로부터 선정된 펩티드 라디칼이며, B'가 라디칼-HisvalproHisvalGly이며, w가 Tyr이고, r이 0또는 1인 일반식(ⅩⅣ)의 이글화합물의 제조방법 및 B가 펩티드라디칼 ThrGlupheGlySerGluLeuLysSerphe이고, B'가 펩티드라디칼-HisvalproHisvalGly이며, w가 His이고, r이 0 또는 1이며, r이 0DLS 일반식(ⅩⅣ)의 화합물의 N-말단의 아미노산이 유리형 또는 N-아세틸화된 일반식(ⅩⅣ)의 이글린 B화합물 및 일반식(ⅩⅣ)의 화합물의 염을 제조하는 방법.
- 제1항에 있어서, B가 N-아세틸-SerGluLeuLysSerphe, ThrGlupheGlySerGluLeuLys Serphe 또는 N-아세틸-ThrGlupheGlySerGluLeuLysSerphe라디칼이며 B'가-HisvalproHisvalGly라디칼이고, w가 Tyr이며, r이 0인 일반식(ⅩⅣ)의 화합물 및 그의 염의 제조방법.
- 제1항에 있어서, 이글린 C의 제조방법.
- 제1항에 있어서, 이글린 B의 제조방법.
- 제1항에 있어서, N-아세틸-이글린 C의 제조방법.
- 제1항에 있어서, 변형된 이글린 C화합물인 이글린 C' 또는 이글린 C″의 제조방법.
- 제1항에 있어서, 효소적 공정에 의해서 r이 0이고 N-말단의 아미노기가 유리형태인 일반식(ⅩⅣ)의 화합물을 N-말단의 아미노산이 N-아세틸화된 상응하는 일반식(ⅩⅣ)의 화합물로 전환시키는 방법.
- 제1항에 있어서, 수득될 수 있는, N-말단에 메티오닐 라디칼을 갖는 일반식(ⅩⅣ′)의 화합물중의 메티오닐 라디칼을 시아노겐 브로마이드로 제거하는 방법.
- 제1항에 있어서, 수득될 수 있는, N-말단에 아세틸화된 아미노기를 갖는 일반식(ⅩⅣ′)의 화합물의 아세틸 라디칼을 효소적으로 제거하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH642283 | 1983-11-21 | ||
| CH1863/84-6 | 1984-04-13 | ||
| CH6422/83-5 | 1984-04-13 | ||
| CH186384 | 1984-04-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR850004791A true KR850004791A (ko) | 1985-07-27 |
Family
ID=25688872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019840007283A KR850004791A (ko) | 1983-11-21 | 1984-11-21 | 프로테아제 억제제의 제조방법 |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20020142414A1 (ko) |
| EP (1) | EP0146785B1 (ko) |
| JP (1) | JPH0730119B2 (ko) |
| KR (1) | KR850004791A (ko) |
| AR (1) | AR241800A1 (ko) |
| CA (1) | CA1297437C (ko) |
| DE (1) | DE3481320D1 (ko) |
| DK (1) | DK550984A (ko) |
| ES (1) | ES8608580A1 (ko) |
| FI (1) | FI86744C (ko) |
| GR (1) | GR80965B (ko) |
| HU (1) | HU203784B (ko) |
| IE (1) | IE57895B1 (ko) |
| IL (1) | IL73569A (ko) |
| NO (1) | NO172547C (ko) |
| NZ (1) | NZ210267A (ko) |
| PT (1) | PT79519B (ko) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61152695A (ja) * | 1984-12-26 | 1986-07-11 | Nippon Shinyaku Co Ltd | 長鎖ｄｎａの合成法 |
| JPS6339414U (ko) * | 1986-08-29 | 1988-03-14 | ||
| DE3789413T2 (de) | 1986-10-03 | 1994-07-28 | Ciba Geigy Ag | Lymphokin-ähnliche Peptide. |
| ES2063161T3 (es) * | 1988-03-07 | 1995-01-01 | Ciba Geigy Ag | Proteinas modificadas. |
| US5180667A (en) * | 1988-03-07 | 1993-01-19 | Ciba-Geigy Corporation | Genes encoding eglin C mutants |
| DE3939801A1 (de) * | 1989-12-01 | 1991-06-06 | Basf Ag | Neue proteine und ihre herstellung |
| US5604201A (en) * | 1993-01-08 | 1997-02-18 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University, A Non-Profit Organization | Methods and reagents for inhibiting furin endoprotease |
| JP2013192526A (ja) * | 2012-03-22 | 2013-09-30 | Sanyo Chem Ind Ltd | タンパク質溶液、このタンパク質溶液のプロテアーゼ活性の回復方法及びこのタンパク質溶液を含有する洗剤組成物 |
| CN108456708A (zh) * | 2018-06-20 | 2018-08-28 | 齐齐哈尔龙江阜丰生物科技有限公司 | 一种发酵制备苏氨酸的培养基 |
| US20230364158A1 (en) * | 2020-10-06 | 2023-11-16 | Mayo Foundation For Medical Education And Research | Methods and materials for treating gastrointestinal disorders |
| US11187700B1 (en) * | 2021-01-28 | 2021-11-30 | Eckhard Kemmann | Closed system for enlarging viral and bacterial particles for identification by diffraction scanning |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2808396C2 (de) | 1978-02-27 | 1984-04-19 | Plantorgan Werk Heinrich G.E. Christensen, KG, 2903 Bad Zwischenahn | Protease-Inhibitoren |
-
1984
- 1984-11-19 PT PT79519A patent/PT79519B/pt not_active IP Right Cessation
- 1984-11-19 FI FI844545A patent/FI86744C/fi not_active IP Right Cessation
- 1984-11-19 GR GR80965A patent/GR80965B/el unknown
- 1984-11-19 CA CA000468119A patent/CA1297437C/en not_active Expired - Lifetime
- 1984-11-20 HU HU844308A patent/HU203784B/hu not_active IP Right Cessation
- 1984-11-20 AR AR84298668A patent/AR241800A1/es active
- 1984-11-20 IL IL73569A patent/IL73569A/xx not_active IP Right Cessation
- 1984-11-20 DE DE8484114022T patent/DE3481320D1/de not_active Expired - Lifetime
- 1984-11-20 NO NO844617A patent/NO172547C/no not_active IP Right Cessation
- 1984-11-20 DK DK550984A patent/DK550984A/da not_active Application Discontinuation
- 1984-11-20 JP JP24353084A patent/JPH0730119B2/ja not_active Expired - Lifetime
- 1984-11-20 IE IE2966/84A patent/IE57895B1/en not_active IP Right Cessation
- 1984-11-20 NZ NZ210267A patent/NZ210267A/xx unknown
- 1984-11-20 EP EP84114022A patent/EP0146785B1/de not_active Expired - Lifetime
- 1984-11-21 ES ES537829A patent/ES8608580A1/es not_active Expired
- 1984-11-21 KR KR1019840007283A patent/KR850004791A/ko not_active Application Discontinuation
-
2001
- 2001-01-22 US US09/765,287 patent/US20020142414A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| PT79519A (en) | 1984-12-01 |
| IL73569A0 (en) | 1985-02-28 |
| NZ210267A (en) | 1988-11-29 |
| IL73569A (en) | 1991-05-12 |
| AU3571884A (en) | 1985-05-30 |
| DK550984A (da) | 1985-05-22 |
| IE57895B1 (en) | 1993-05-05 |
| NO844617L (no) | 1985-05-22 |
| DE3481320D1 (de) | 1990-03-15 |
| CA1297437C (en) | 1992-03-17 |
| PT79519B (en) | 1986-12-11 |
| AU596347B2 (en) | 1990-05-03 |
| HUT36864A (en) | 1985-10-28 |
| FI86744C (fi) | 1992-10-12 |
| NO172547B (no) | 1993-04-26 |
| ES537829A0 (es) | 1986-06-16 |
| ES8608580A1 (es) | 1986-06-16 |
| EP0146785B1 (de) | 1990-02-07 |
| IE842966L (en) | 1985-05-21 |
| AR241800A1 (es) | 1992-12-30 |
| EP0146785A1 (de) | 1985-07-03 |
| JPS60192592A (ja) | 1985-10-01 |
| DK550984D0 (da) | 1984-11-20 |
| FI86744B (fi) | 1992-06-30 |
| US20020142414A1 (en) | 2002-10-03 |
| FI844545L (fi) | 1985-05-22 |
| NO172547C (no) | 1993-08-04 |
| JPH0730119B2 (ja) | 1995-04-05 |
| FI844545A0 (fi) | 1984-11-19 |
| HU203784B (en) | 1991-09-30 |
| GR80965B (en) | 1985-03-20 |
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