JP3162091B2 - ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体 - Google Patents

ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体

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Publication number
JP3162091B2
JP3162091B2 JP03323691A JP3323691A JP3162091B2 JP 3162091 B2 JP3162091 B2 JP 3162091B2 JP 03323691 A JP03323691 A JP 03323691A JP 3323691 A JP3323691 A JP 3323691A JP 3162091 B2 JP3162091 B2 JP 3162091B2
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Japan
Prior art keywords
seq
dna
subunit
sequence
nitrile hydratase
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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JP03323691A
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English (en)
Japanese (ja)
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JPH04211379A (ja
Inventor
輝彦 別府
秀明 山田
真 西山
透 長沢
末治 堀之内
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輝彦 別府
秀明 山田
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Publication of JPH04211379A publication Critical patent/JPH04211379A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP03323691A 1990-02-28 1991-02-27 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体 Expired - Lifetime JP3162091B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP4807890 1990-02-28
JP2-48078 1990-02-28
US08/028,463 US5731176A (en) 1990-02-28 1993-03-09 DNA fragment encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant

Publications (2)

Publication Number Publication Date
JPH04211379A JPH04211379A (ja) 1992-08-03
JP3162091B2 true JP3162091B2 (ja) 2001-04-25

Family

ID=26388301

Family Applications (1)

Application Number Title Priority Date Filing Date
JP03323691A Expired - Lifetime JP3162091B2 (ja) 1990-02-28 1991-02-27 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体

Country Status (18)

Country Link
US (1) US5731176A (zh)
EP (1) EP0445646B1 (zh)
JP (1) JP3162091B2 (zh)
KR (1) KR950002004B1 (zh)
CN (1) CN1054639C (zh)
AT (1) ATE138101T1 (zh)
AU (1) AU627648B2 (zh)
CA (1) CA2037291C (zh)
CZ (2) CZ284048B6 (zh)
DE (1) DE69119454T2 (zh)
DK (1) DK0445646T3 (zh)
ES (1) ES2089044T3 (zh)
FI (1) FI102539B1 (zh)
MX (1) MX193790B (zh)
NO (1) NO306684B1 (zh)
PL (1) PL166201B1 (zh)
RU (1) RU2081173C1 (zh)
SK (1) SK280199B6 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012164933A1 (ja) 2011-05-31 2012-12-06 ダイヤニトリックス株式会社 改良型ニトリルヒドラターゼ
WO2012169203A1 (ja) 2011-06-07 2012-12-13 ダイヤニトリックス株式会社 改良型ニトリルヒドラターゼ
KR20160145183A (ko) 2014-06-06 2016-12-19 미쯔비시 레이온 가부시끼가이샤 개량형 니트릴 히드라타제

Families Citing this family (27)

* Cited by examiner, † Cited by third party
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JP2907479B2 (ja) * 1990-02-28 1999-06-21 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体及びアミド類の製造法
EP0502476B1 (en) * 1991-03-04 2001-07-18 Mitsubishi Rayon Co., Ltd. Hybrid plasmid vectors containing genes encoding nitrile degrading enzymes and methods of producing amides and acids
AU3692593A (en) * 1992-04-28 1993-11-04 Mitsubishi Kasei Corporation Novel protein having nitrile hydratase activity and the gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene
JP3828590B2 (ja) * 1994-10-03 2006-10-04 昌 清水 ニトリルヒドラターゼ遺伝子発現のための調節遺伝子
JP3154633B2 (ja) * 1994-12-28 2001-04-09 三菱レイヨン株式会社 ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子
AU7514896A (en) * 1995-10-06 1997-04-28 E.I. Du Pont De Nemours And Company Nucleic acid fragments encoding stereospecific nitrile hydratase and amidase enzymes and recombinant microorganisms expressing those enzymes useful for the production of chiral amides and acides
CN1260363C (zh) * 1996-02-14 2006-06-21 三井化学株式会社 由腈化合物生产酰胺的方法
US5866379A (en) * 1997-01-28 1999-02-02 Novus International Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha.
FR2770214B1 (fr) * 1997-10-24 1999-12-31 Rhodia Chimie Sa Procede de preparation d'un benzamide halogene
JP2000050866A (ja) * 1998-08-06 2000-02-22 Rikagaku Kenkyusho ニトリルヒドラターゼの生産方法
JP4185607B2 (ja) * 1998-12-15 2008-11-26 ダイセル化学工業株式会社 新規な微生物及びアミド化合物の製造方法
CN1340101A (zh) * 1999-10-26 2002-03-13 昭和电工株式会社 新型红球菌属细菌、源自于红球菌属细菌的腈水解酶基因、腈水合酶基因和酰胺酶基因,以及利用它们生产羧酸的方法
KR100547080B1 (ko) 2000-12-20 2006-01-31 다이야니트릭스 가부시키가이샤 미생물 촉매를 이용한 아미드 화합물의 제조 방법
CA2432060C (en) * 2001-01-09 2013-04-23 Lonza Ag Microbiological method for producing amides
JP2002325587A (ja) * 2001-03-02 2002-11-12 Daicel Chem Ind Ltd ニトリルヒドラターゼ、およびアミドの製造方法
JP2004194588A (ja) 2002-12-19 2004-07-15 Mitsui Chemicals Inc 新規なニトリルヒドラターゼ
WO2004067738A2 (en) * 2003-01-27 2004-08-12 Degussa Ag Nitrile hydratases from rhodococcus erythropolis and their application
BRPI0417244B1 (pt) 2003-12-02 2015-05-26 Ciba Spec Chem Water Treat Ltd Métodos para cultivo e armazenamento de rhodococcus rhodochrous, bem como para melhorar atividade biocatalítica do mesmo, e processo para preparação de amida
CN102604921B (zh) * 2004-05-26 2014-12-03 Dia-Nitrix株式会社 改良型腈水合酶
JP2006187257A (ja) 2005-01-07 2006-07-20 Daiyanitorikkusu Kk アミド化合物の製造方法およびアクリルアミド系ポリマー
EP1842907A1 (en) 2006-04-07 2007-10-10 B.R.A.I.N. Ag A group of novel enantioselective microbial nitrile hydratases with broad substrate specificity
WO2008004473A1 (fr) * 2006-07-06 2008-01-10 University Of Tsukuba Nouveau complexe de protéines, procédé de maturation de nitrile hydratase à faible poids moléculaire de type au cobalt utilisant le complexe de protéines, nitrile hydratase correspondante et procédé l'utilisant
US8334132B2 (en) 2006-11-09 2012-12-18 Mitsubishi Rayon Co., Ltd. Process for production of a betaine such as carnitine
FR3002542B1 (fr) * 2013-02-28 2016-01-22 Servier Lab Procede de synthese enzymatique de l'acide (7s) 3,4-dimethoxybicyclo[4.2.0]octa-1,3,5-triene 7-carboxylique et application a la synthese de l'ivabradine et de ses sels
WO2018085493A1 (en) * 2016-11-04 2018-05-11 Georgia State University Research Foundation, Inc. Endotoxin free asparaginase
WO2022172880A1 (ja) 2021-02-10 2022-08-18 三菱ケミカル株式会社 アルデヒドによるニトリルヒドラターゼの反応性向上
CN114934006A (zh) * 2022-06-02 2022-08-23 无锡新晨宇生物工程有限公司 一种腈水合酶催化乙腈生成乙酰胺的应用

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* Cited by examiner, † Cited by third party
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MX169933B (es) * 1987-09-18 1993-08-02 Hideaki Yamada Procedimiento para la produccion biologica de amidas
JP2840253B2 (ja) * 1988-07-06 1998-12-24 輝彦 別府 ニトリルヒドラターゼ活性を有するポリペプチドをコードする遺伝子ｄｎａ、これを含有する形質転換体によるニトリル類からアミド類の製造法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Ｂｉｏｃｈｅｍ．Ｂｉｏｐｈｙｓ．Ｒｅｓ．Ｃｏｍｍｕｎ．，１５５（２）ｐｐ．１００８−１０１６（１９８８）
Ｅｕｒ．Ｊ．Ｂｉｏｃｈｅｍ．，１８１（３），ｐｐ．５６３−５７０（１９８９）

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012164933A1 (ja) 2011-05-31 2012-12-06 ダイヤニトリックス株式会社 改良型ニトリルヒドラターゼ
US9388403B2 (en) 2011-05-31 2016-07-12 Mitsubishi Rayon Co., Ltd. Nitrile hydratase
US9382528B2 (en) 2011-05-31 2016-07-05 Mitsubishi Rayon Co., Ltd. Nitrile hydratase
KR20150092767A (ko) 2011-06-07 2015-08-13 미쯔비시 레이온 가부시끼가이샤 개량형 니트릴 히드라타제
KR20150092768A (ko) 2011-06-07 2015-08-13 미쯔비시 레이온 가부시끼가이샤 개량형 니트릴 히드라타제
KR20150092770A (ko) 2011-06-07 2015-08-13 미쯔비시 레이온 가부시끼가이샤 개량형 니트릴 히드라타제
KR20150092769A (ko) 2011-06-07 2015-08-13 미쯔비시 레이온 가부시끼가이샤 개량형 니트릴 히드라타제
US9193966B2 (en) 2011-06-07 2015-11-24 Mitsubishi Rayon Co., Ltd. Nitrile hydratase
EP2811021A2 (en) 2011-06-07 2014-12-10 Mitsubishi Rayon Co., Ltd. Improved nitrile hydratase
WO2012169203A1 (ja) 2011-06-07 2012-12-13 ダイヤニトリックス株式会社 改良型ニトリルヒドラターゼ
US9738885B2 (en) 2011-06-07 2017-08-22 Mitsubishi Chemical Corporation Nitrile hydratase
US10066225B2 (en) 2011-06-07 2018-09-04 Mitsubishi Chemical Corporation Nitrile hydratase
US10221410B2 (en) 2011-06-07 2019-03-05 Mitsubishi Chemical Corporation Nitrile hydratase
US10487320B2 (en) 2011-06-07 2019-11-26 Mitsubishi Chemical Corporation Nitrile hydratase
KR20160145183A (ko) 2014-06-06 2016-12-19 미쯔비시 레이온 가부시끼가이샤 개량형 니트릴 히드라타제
US10093912B2 (en) 2014-06-06 2018-10-09 Mitsubishi Chemical Corporation Nitrile hydratase

Also Published As

Publication number Publication date
JPH04211379A (ja) 1992-08-03
EP0445646A3 (en) 1992-01-08
NO306684B1 (no) 1999-12-06
RU2081173C1 (ru) 1997-06-10
KR910021473A (ko) 1991-12-20
KR950002004B1 (ko) 1995-03-08
DE69119454D1 (de) 1996-06-20
CZ317094A3 (cs) 1998-05-13
NO910797L (no) 1991-08-29
CN1054616A (zh) 1991-09-18
PL166201B1 (pl) 1995-04-28
ES2089044T3 (es) 1996-10-01
PL289239A1 (en) 1992-03-23
SK280199B6 (sk) 1999-09-10
EP0445646B1 (en) 1996-05-15
ATE138101T1 (de) 1996-06-15
FI102539B (fi) 1998-12-31
MX193790B (en) 1999-10-19
CZ284048B6 (cs) 1998-07-15
CN1054639C (zh) 2000-07-19
AU627648B2 (en) 1992-08-27
CA2037291A1 (en) 1991-08-29
FI910960A0 (fi) 1991-02-27
FI910960A (fi) 1991-08-29
AU7129391A (en) 1991-09-12
NO910797D0 (no) 1991-02-27
US5731176A (en) 1998-03-24
DK0445646T3 (da) 1996-09-30
CS9100531A2 (en) 1991-10-15
FI102539B1 (fi) 1998-12-31
CZ284017B6 (cs) 1998-07-15
DE69119454T2 (de) 1996-09-26
EP0445646A2 (en) 1991-09-11
CA2037291C (en) 2001-07-24

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