MX389743B - Proceso mejorado para la producción de oligosacáridos fucosilados. - Google Patents

Proceso mejorado para la producción de oligosacáridos fucosilados.

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Publication number
MX389743B
MX389743B MX2019004867A MX2019004867A MX389743B MX 389743 B MX389743 B MX 389743B MX 2019004867 A MX2019004867 A MX 2019004867A MX 2019004867 A MX2019004867 A MX 2019004867A MX 389743 B MX389743 B MX 389743B
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MX
Mexico
Prior art keywords
host cell
production
fucosylated oligosaccharides
improved process
fucosylated
Prior art date
Application number
MX2019004867A
Other languages
English (en)
Other versions
MX2019004867A (es
Inventor
Dirk Wartenberg
Katja Parschat
Stefan Jennewein
Original Assignee
Chr Hansen Hmo Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=57256077&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX389743(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Chr Hansen Hmo Gmbh filed Critical Chr Hansen Hmo Gmbh
Publication of MX2019004867A publication Critical patent/MX2019004867A/es
Publication of MX389743B publication Critical patent/MX389743B/es

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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01271—GDP-L-fucose synthase (1.1.1.271)
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    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01065—3-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01011—6-Phosphofructokinase (2.7.1.11)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07013—Mannose-1-phosphate guanylyltransferase (2.7.7.13)
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03011—Fructose-bisphosphatase (3.1.3.11)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/02—Aldehyde-lyases (4.1.2)
    • C12Y401/02013—Fructose-bisphosphate aldolase (4.1.2.13)
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    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01047—GDP-mannose 4,6-dehydratase (4.2.1.47), i.e. GMD
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    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02008—Phosphomannomutase (5.4.2.8)
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    • C12Y204/01—Hexosyltransferases (2.4.1)
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    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01152—4-Galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase (2.4.1.152)

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  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

La presente invención se refiere a un método para producir oligosacáridos fucosilados usando una célula hospedera procariota recombinante que se cultiva sobre un sustrato gluconeogénico, y también la célula hospedera y su uso. La célula hospedera es modificada genéticamente para que la actividad de una enzima convertidora de fructosa-6-fosfato sea anulada o reducida, y el transporte del oligosacárido fucosilado producido a través de la membrana celular es facilitado por una proteína de transporte exógena.
MX2019004867A 2016-10-29 2017-10-24 Proceso mejorado para la producción de oligosacáridos fucosilados. MX389743B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP16196486.1A EP3315610B1 (en) 2016-10-29 2016-10-29 Process for the production of fucosylated oligosaccharides
PCT/EP2017/077192 WO2018077892A1 (en) 2016-10-29 2017-10-24 Improved process for the production of fucosylated oligosaccharides

Publications (2)

Publication Number Publication Date
MX2019004867A MX2019004867A (es) 2019-08-14
MX389743B true MX389743B (es) 2025-03-20

Family

ID=57256077

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019004867A MX389743B (es) 2016-10-29 2017-10-24 Proceso mejorado para la producción de oligosacáridos fucosilados.

Country Status (15)

Country Link
US (2) US11898185B2 (es)
EP (2) EP3315610B1 (es)
JP (2) JP2019531752A (es)
KR (1) KR102554781B1 (es)
CN (1) CN109790559A (es)
AU (1) AU2017351657B2 (es)
BR (1) BR112019008585A2 (es)
DK (1) DK3315610T3 (es)
ES (1) ES2856749T3 (es)
MX (1) MX389743B (es)
PH (1) PH12019550056A1 (es)
PL (1) PL3315610T3 (es)
SG (1) SG11201903024YA (es)
TW (1) TWI799395B (es)
WO (1) WO2018077892A1 (es)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL3315610T3 (pl) 2016-10-29 2021-06-14 Jennewein Biotechnologie Gmbh Sposób wytwarzania fukozylowanych oligosacharydów
ES2928859T3 (es) * 2017-03-17 2022-11-23 Chr Hansen Hmo Gmbh Un método para inhibir la isomerización de un sacárido reductor tras el tratamiento térmico
US11512318B2 (en) * 2017-04-21 2022-11-29 Seoul National University R&Db Foundation Method for producing 3-fucosyllactose using Corynebacterium glutamicum
US12077792B2 (en) * 2018-05-15 2024-09-03 The Board Of Trustees Of The University Of Illinois Engineered microorganisms for production of 2′fucosyllactose and l-fucose
EP3830281A1 (en) * 2018-07-27 2021-06-09 Global Bioenergies Method for producing fructose-6-phosphate from dihydroxyacetone phosphate and glyceraldehyde-3-phosphate
EP3620510B1 (en) 2018-09-06 2023-11-01 Chr. Hansen HMO GmbH Fermentative production of oligosaccharides by total fermentation utilizing a mixed feedstock
CN109402158B (zh) * 2018-09-14 2022-01-11 江苏大学 一种产岩藻糖基乳糖的重组表达质粒载体、代谢工程菌及生产方法
JP2022512574A (ja) * 2018-10-02 2022-02-07 ジミテック，インコーポレーテッド オリゴ糖のエクスポートのための基質インポーターの使用
AU2019409833A1 (en) * 2018-12-18 2021-08-05 Inbiose N.V. Production of 3-fucosyllactose and lactose converting alpha-1,3-fucosyltransferase enzymes
EP3908302A1 (en) 2019-01-10 2021-11-17 Expres2ion Biotechnologies ApS Glyco-engineered immunization antigens
CN109749976B (zh) * 2019-01-30 2022-04-01 光明乳业股份有限公司 一种高效合成鸟苷二磷酸岩藻糖的重组枯草芽孢杆菌及其构建方法与应用
CN110172486A (zh) * 2019-05-14 2019-08-27 天津科技大学 一种合成2’-岩藻糖基乳糖的方法
US20220259631A1 (en) * 2019-07-19 2022-08-18 Inbiose N.V. Production of fucosyllactose in host cells
KR102154256B1 (ko) * 2019-07-30 2020-09-10 서울대학교산학협력단 코리네박테리움 글루타미쿰을 이용한 3'-푸코실락토오스의 생산방법
KR102268092B1 (ko) * 2019-09-02 2021-06-25 서울대학교산학협력단 코리네박테리움 글루타미쿰에 푸코실락토오스 수송체 도입과 gdp-l-푸코오스 생합성 경로의 최적화를 통한 2'-푸코실락토오스 생산 증대
BR112022005578A2 (pt) 2019-09-24 2022-09-20 Prolacta Bioscience Inc Composições e métodos para tratamento de doenças inflamatórias e imunes
JP6917434B2 (ja) * 2019-10-25 2021-08-11 長瀬産業株式会社 好気性菌の代謝酵素破壊株およびその培養法
CN110804577B (zh) * 2019-11-28 2021-05-28 江南大学 一种高效生产2’-岩藻糖基乳糖的重组菌的构建方法及其应用
JP7697887B2 (ja) * 2019-12-16 2025-06-24 協和発酵バイオ株式会社 改変されたラクトースパーミアーゼを有する微生物及びラクトース含有オリゴ糖の製造法
AU2020403864A1 (en) * 2019-12-17 2022-08-11 Inbiose N.V. Lactose converting alpha-1,2-fucosyltransferase enzymes
CN114981291B (zh) 2020-01-23 2024-12-06 格礼卡姆股份公司 Hmo产生
JP2023511522A (ja) * 2020-01-23 2023-03-20 グリコム・アクティーゼルスカブ Ｈｍｏの産生
BR112022014414A2 (pt) * 2020-01-23 2022-09-13 Glycom As Produção de hmo
US20230072639A1 (en) 2020-01-23 2023-03-09 Glycom A/S New major facilitator superfamily (mfs) protein (bad) in hmo production
AU2021219964A1 (en) * 2020-02-14 2022-09-29 Inbiose N.V. Production of glycosylated product in host cells
KR20220154158A (ko) 2020-03-12 2022-11-21 글리콤 에이/에스 2'-fl의 결정화
CN111471637A (zh) * 2020-05-08 2020-07-31 江苏华燕集团有限公司 2`-岩藻糖基乳糖高产菌株及其制备方法和用途
CN111575220B (zh) * 2020-05-25 2023-06-02 江南大学 合成2’-岩藻糖基乳糖的重组大肠杆菌及其构建方法与应用
CN111549013A (zh) * 2020-06-09 2020-08-18 中国科学院天津工业生物技术研究所 一种atp依赖的甘露糖激酶及其在岩藻基乳糖合成中的应用
EP3929300A1 (en) * 2020-06-26 2021-12-29 Chr. Hansen HMO GmbH Improved export of oligosaccharides from bacterial cells
CN116234919A (zh) * 2020-08-10 2023-06-06 因比奥斯公司 借由细胞制造中性岩藻糖基化寡糖的混合物
CN116322371A (zh) 2020-08-14 2023-06-23 普罗莱克塔生物科学公司 与细菌疗法一起使用的人乳寡糖组合物
CN114438001B (zh) * 2020-11-05 2022-11-15 中国科学院上海高等研究院 生产3-岩藻糖基乳糖的重组枯草芽孢杆菌及其构建方法和应用
CN112574936A (zh) * 2020-12-21 2021-03-30 中国科学院合肥物质科学研究院 一种重组大肠杆菌及其构建方法与应用
CN112625987B (zh) * 2020-12-21 2023-01-06 南通励成生物工程有限公司 一种同时生产2`-岩藻糖基乳糖和d-阿洛酮糖的方法
DK180952B1 (en) 2020-12-22 2022-08-10 Glycom As A dfl-producing strain
CN112662604B (zh) * 2020-12-29 2023-10-20 量子高科(广东)生物有限公司 一种合成3-岩藻糖基乳糖的重组大肠杆菌及其构建方法
KR102793833B1 (ko) * 2020-12-31 2025-04-11 주식회사 삼양사 푸코오스 전이효소를 발현하는 재조합 미생물 및 이를 이용한 2’-푸코실락토오스 제조방법
KR102633804B1 (ko) * 2020-12-31 2024-02-05 주식회사 삼양사 재조합 바실러스 속 미생물 및 이를 이용한 모유올리고당 제조방법
MX2023008227A (es) 2021-01-12 2023-07-20 Prolacta Bioscience Inc Regimenes de tratamiento simbiotico.
EP4281564A1 (en) 2021-01-22 2023-11-29 Glycom A/S New major facilitator superfamily (mfs) protein (fred) in production of sialylated hmos
US20240110212A1 (en) * 2021-02-08 2024-04-04 Kyowa Hakko Bio Co., Ltd. Protein having fucose-containing sugar transporting activity, and method for producing fucose-containing sugar
US20240301456A1 (en) * 2021-02-08 2024-09-12 Kyowa Hakko Bio Co., Ltd. Protein having 1,3-fucosyltransferase activity, and method for producing fucose-containing sugar
DK181242B1 (en) 2021-05-17 2023-05-30 Dsm Ip Assets Bv GENETICALLY ENGINEERED CELLS COMPRISING A RECOMBINANT NUCLEIC ACID SEQUNCE ENCODING AN α-1,2-FUCOSYLTRANSFERASE CAPABLE OF PRODUCING LNFP-I, NUCLEIC ACID SEQUENCES ENCODING SAME AND METHODS FOR USE OF SAME
KR102477273B1 (ko) * 2021-11-24 2022-12-16 (주)에이피테크놀로지 효소 처리를 통한 2'-푸코실락토오스의 생산성 증대 방법
CN116179449A (zh) 2021-11-26 2023-05-30 虹摹生物科技(上海)有限公司 一种lacZ失活的基因工程菌及其在人乳寡糖生产中的应用
CN114480240B (zh) * 2022-02-22 2024-03-26 江南大学 一种产岩藻糖基乳糖的基因工程菌及生产方法
CN114645007B (zh) * 2022-03-11 2023-09-12 南通励成生物工程有限公司 一种重组菌株及基于此的高产2’-岩藻糖基乳糖的工程菌及工程菌的应用
CN116064346B (zh) * 2022-08-16 2025-08-22 江南大学 一种高效合成乳糖的方法及其应用
AU2023331863A1 (en) 2022-08-29 2025-02-20 Chr. Hansen A/S Fermentative production of oligosaccharides by microbial cells utilizing glycerol
WO2024053959A1 (ko) * 2022-09-06 2024-03-14 고려대학교 산학협력단 콜라닉산으로부터 퓨코실화 올리고당의 생산 방법
WO2024123084A1 (ko) * 2022-12-06 2024-06-13 (주)에이피테크놀로지 베타-갈락토시다아제가 발현되도록 형질전환된 재조합 코리네박테리움 글루타미쿰을 이용한 푸코실락토오스의 생산방법
WO2024130119A2 (en) 2022-12-16 2024-06-20 Prolacta Bioscience, Inc. Synbiotic compositions for short chain fatty acid production
CN116948928B (zh) * 2023-06-02 2024-06-18 虹摹生物科技(上海)有限公司 种子培养基及无抗生素、无iptg诱导剂的2’-岩藻糖基乳糖的发酵生产方法
CN119101639B (zh) * 2023-10-31 2025-10-24 汤臣倍健股份有限公司 一种合成岩藻糖基乳糖的基因工程菌及其制备方法和应用
WO2025113319A1 (zh) * 2023-11-30 2025-06-05 山东恒鲁生物科技有限公司 一种gdp-甘露糖脱水酶多肽及其应用
CN117467594B (zh) * 2023-12-26 2024-04-09 态创生物科技(广州)有限公司 一种生产2'-岩藻糖基乳糖的基因工程菌及其应用
CN118931812B (zh) * 2024-07-25 2025-03-04 合肥中科健康生物产业技术研究院有限公司 一种高产岩藻糖的大肠杆菌及其构建方法和应用
CN119162072B (zh) * 2024-10-14 2025-08-08 合肥中科健康生物产业技术研究院有限公司 一种在岩藻糖合成过程中降低乳糖残留的工程菌及其构建方法和应用
CN119220476B (zh) * 2024-12-03 2025-02-28 天津合生领航生物科技有限公司 一种提高二岩藻糖基乳糖产量的重组大肠杆菌构建及应用

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6821756B2 (en) 1996-09-17 2004-11-23 Kyowa Hakko Kogyo Co., Ltd. Processes for reproducing sugar nucleotides and complex carbohydrates
FR2796082B1 (fr) * 1999-07-07 2003-06-27 Centre Nat Rech Scient Procede de production d'oligosaccharides
DE102005022045A1 (de) 2005-05-09 2006-11-16 Rwth Aachen Fermentationsverfahren und Anordnung zu dessen Durchführung
EP1927316B1 (en) 2006-12-01 2012-10-17 Radi Medical Systems Ab Sensor and guide wire assembly
RU2584599C2 (ru) 2008-12-19 2016-05-20 Дженневейн Биотехнологие Гмбх Синтез фукозилированных соединений
ES2660698T3 (es) 2009-06-08 2018-03-23 Jennewein Biotechnologie Gmbh Síntesis de HMO
MX386398B (es) * 2010-07-12 2025-03-18 Inbiose Nv Organismos metabólicamente diseñados para la producción de bioproductos de valor agregado.
DK2439264T3 (en) 2010-10-11 2015-08-03 Jennewein Biotechnologie Gmbh Novel fucosyltransferases and their use
DK2479263T3 (da) 2011-01-20 2014-02-03 Jennewein Biotechnologie Gmbh Nye fucosyltransferaser og deres anvendelser
US9453230B2 (en) 2011-02-16 2016-09-27 Glycosyn LLC Biosynthesis of human milk oligosaccharides in engineered bacteria
CN103703123B (zh) * 2011-07-12 2016-08-17 财富科学家股份有限公司 用于产生有用代谢物的重组微生物
EP3517631B9 (en) 2011-12-16 2023-10-04 Inbiose N.V. Mutant microorganisms to synthesize ph sensitive molecules and organic acids
US9029136B2 (en) 2012-07-25 2015-05-12 Glycosyn LLC Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides
DK2728009T3 (da) 2012-10-31 2017-11-06 Jennewein Biotechnologie Gmbh Fremgangsmåde til fremstilling af monosaccharider
DK2845905T3 (da) 2013-09-10 2021-06-14 Chr Hansen Hmo Gmbh Fremstilling af oligosaccharider
DK2927316T3 (en) 2014-03-31 2019-03-04 Jennewein Biotechnologie Gmbh Total fermentation of oligosaccharides
CN106460024B (zh) 2014-06-27 2021-10-01 格礼卡姆股份公司 寡糖的制备
CA2960835A1 (en) 2014-09-09 2016-03-17 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides
BR112017005665A2 (pt) * 2014-09-18 2017-12-12 Genomatica Inc organismos microbianos não naturais com eficiência energética melhorada
EP3218509B1 (en) * 2014-11-14 2024-02-28 Inbiose N.V. Mutant microorganisms resistant to lactose killing
KR101544184B1 (ko) 2014-12-19 2015-08-21 서울대학교산학협력단 2-푸코실락토오스 생산 변이 미생물 및 이를 이용한 2-푸코실락토오스의 제조방법
EP3050973A1 (en) 2015-01-30 2016-08-03 Jennewein Biotechnologie GmbH Fermentation process for producing monosaccharides in free form from nucleotide-activated sugars
WO2016153300A1 (ko) * 2015-03-24 2016-09-29 서울대학교 산학협력단 2-푸코실락토오스 생산 변이 미생물 및 이를 이용한 2-푸코실락토오스의 제조방법
PL3315610T3 (pl) 2016-10-29 2021-06-14 Jennewein Biotechnologie Gmbh Sposób wytwarzania fukozylowanych oligosacharydów

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