WO2015199387A3 - 가용성 단백질 발현량 및 활성이 증대된 헬리코박터 파일로리 유래 α-1,3 푸코실 전달효소의 유전자와 단백질 및 α-1,3 푸코실올리고당 생산에의 응용 - Google Patents

가용성 단백질 발현량 및 활성이 증대된 헬리코박터 파일로리 유래 α-1,3 푸코실 전달효소의 유전자와 단백질 및 α-1,3 푸코실올리고당 생산에의 응용 Download PDF

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WO2015199387A3
WO2015199387A3 PCT/KR2015/006272 KR2015006272W WO2015199387A3 WO 2015199387 A3 WO2015199387 A3 WO 2015199387A3 KR 2015006272 W KR2015006272 W KR 2015006272W WO 2015199387 A3 WO2015199387 A3 WO 2015199387A3
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fucosyloligosaccharide
production
application
soluble protein
helicobacter pylori
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PCT/KR2015/006272
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French (fr)
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WO2015199387A2 (ko
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김병기
최윤희
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서울대학교산학협력단
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Priority to US15/322,072 priority Critical patent/US10336990B2/en
Publication of WO2015199387A2 publication Critical patent/WO2015199387A2/ko
Publication of WO2015199387A3 publication Critical patent/WO2015199387A3/ko

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12P19/00—Preparation of compounds containing saccharide radicals
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
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    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01065—3-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91091—Glycosyltransferases (2.4)
    • G01N2333/91097—Hexosyltransferases (general) (2.4.1)
    • G01N2333/91102—Hexosyltransferases (general) (2.4.1) with definite EC number (2.4.1.-)

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Abstract

본 발명은 가용성 단백질 발현량 및 활성이 증대된 α-1,3 푸코실 전달효소 변이체, α-1,3 푸코실 전달효소 변이체를 코딩하는 DNA, α-1,3 푸코실 전달효소 변이체를 코딩하는 DNA를 포함하는 재조합 DNA 벡터, 상기 재조합 DNA 벡터로 형질전환된 숙주세포 및 숙주세포의 추출물, 3-푸코실올리고당의 제조 방법, 푸코실 전달효소 변이체 제조방법 및 푸코실 전달효소의 변이체 탐색방법을 제공하는 것으로서, 본 발명의 α-1,3 푸코실 전달효소 변이체는 가용성 단백질의 발현량 및 효소 활성이 획기적으로 향상된 효과가 있다.
PCT/KR2015/006272 2014-06-23 2015-06-22 가용성 단백질 발현량 및 활성이 증대된 헬리코박터 파일로리 유래 α-1,3 푸코실 전달효소의 유전자와 단백질 및 α-1,3 푸코실올리고당 생산에의 응용 WO2015199387A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US15/322,072 US10336990B2 (en) 2014-06-23 2015-06-22 Helicobacter pylori α-1,3 fucosyltransferase gene and protein with improved soluble protein expression and activity, and thereof application for synthesis of α-1,3 fucosyloligosaccharide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2014-0076720 2014-06-23
KR1020140076720A KR101718681B1 (ko) 2014-06-23 2014-06-23 가용성 단백질 발현량 및 활성이 증대된 헬리코박터 파일로리 유래 α-1,3 푸코실 전달효소의 유전자와 단백질 및 α-1,3 푸코실올리고당 생산에의 응용

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WO2015199387A2 WO2015199387A2 (ko) 2015-12-30
WO2015199387A3 true WO2015199387A3 (ko) 2016-03-10

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PCT/KR2015/006272 WO2015199387A2 (ko) 2014-06-23 2015-06-22 가용성 단백질 발현량 및 활성이 증대된 헬리코박터 파일로리 유래 α-1,3 푸코실 전달효소의 유전자와 단백질 및 α-1,3 푸코실올리고당 생산에의 응용

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US (1) US10336990B2 (ko)
KR (1) KR101718681B1 (ko)
WO (1) WO2015199387A2 (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101718681B1 (ko) 2014-06-23 2017-03-22 서울대학교산학협력단 가용성 단백질 발현량 및 활성이 증대된 헬리코박터 파일로리 유래 α-1,3 푸코실 전달효소의 유전자와 단백질 및 α-1,3 푸코실올리고당 생산에의 응용
KR101731263B1 (ko) * 2016-04-25 2017-05-02 서울대학교 산학협력단 코리네박테리움 글루타미쿰을 이용한 2'-푸코실락토오스의 생산방법
US11162133B2 (en) * 2016-06-03 2021-11-02 New England Biolabs, Inc. Detection of an amplification product using pH-sensitive dye
CN108103039B (zh) * 2016-11-25 2021-10-22 上海交通大学 一组岩藻糖基转移酶突变体及其筛选方法和应用
DK180952B1 (en) * 2020-12-22 2022-08-10 Glycom As A dfl-producing strain
JPWO2022168992A1 (ko) 2021-02-08 2022-08-11

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399337B1 (en) 1997-06-06 2002-06-04 The Governors Of The University Of Alberta α1,3-fucosyltransferase
US7214517B2 (en) 2001-08-24 2007-05-08 Kyowa Hakko Kogyo Co., Ltd. α1,2-fucosyltransferase and DNA encoding the same
US7326770B2 (en) 2004-01-22 2008-02-05 Neose Technologies, Inc. H. pylori fucosyltransferases
DK2944690T3 (en) 2010-10-11 2018-04-03 Jennewein Biotechnologie Gmbh UNKNOWN FUCOSYL TRANSFERASES AND THEIR APPLICATIONS
CA2827313C (en) * 2011-02-16 2023-08-22 Glycosyn LLC Biosynthesis of human milk oligosaccharides in engineered bacteria
KR101718681B1 (ko) 2014-06-23 2017-03-22 서울대학교산학협력단 가용성 단백질 발현량 및 활성이 증대된 헬리코박터 파일로리 유래 α-1,3 푸코실 전달효소의 유전자와 단백질 및 α-1,3 푸코실올리고당 생산에의 응용

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ALBESA-JOVE, D. ET AL.: "Structure-function relationships of membrane-associated GT-B glycosyltransferases.", GLYCOBIOLOGY, vol. 24, no. 2, 18 November 2013 (2013-11-18), pages 108 - 124 *
DATABASE NCBI 15 May 2013 (2013-05-15), "fucasyltransferase [Helicobacter pylori", Database accession no. WP_000487428 *
SUN, HY. ET AL.: "Structure and mechanism of Helicobacter pylori fucosyltransferase. A basis for lipopotysaccharide variation and inhibitor design.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 282, no. 13, 30 March 2007 (2007-03-30), pages 9973 - 9982, XP055200014, DOI: doi:10.1074/jbc.M610285200 *

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KR20150146301A (ko) 2015-12-31
US20170247668A1 (en) 2017-08-31
WO2015199387A2 (ko) 2015-12-30
KR101718681B1 (ko) 2017-03-22
US10336990B2 (en) 2019-07-02

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