MX166885B - Metodos de purificacion y reactivacion de proteinas heterologas precipitadas - Google Patents

Metodos de purificacion y reactivacion de proteinas heterologas precipitadas

Info

Publication number
MX166885B
MX166885B MX199852A MX19985283A MX166885B MX 166885 B MX166885 B MX 166885B MX 199852 A MX199852 A MX 199852A MX 19985283 A MX19985283 A MX 19985283A MX 166885 B MX166885 B MX 166885B
Authority
MX
Mexico
Prior art keywords
host cell
refractile
protein
reactivation
precipitated
Prior art date
Application number
MX199852A
Other languages
English (en)
Inventor
Kenneth Charles Olson
Rong-Chang Pai
Steven Jacob Shire
Ronald Burnell Wetzel
Andrew Julian Stuart Jones
Stuart Elliot Builder
Norm Shin Chsiang Lin
John Robert Ogez
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27575443&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX166885(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX166885B publication Critical patent/MX166885B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

La presente invención se refiere a un método para tratar un producto de expresión heterólogo a partir de un cultivo de células huéspedes, el cual comprende poner en contacto un material refráctil, que contiene dicho producto, con una solución de desnaturalización. Un método para extraer material refráctil que incluye proteína heteróloga de material de células huéspedes, el cual comprende: i) suspender el material en una solución tamponada; ii) romper el material celular a manera de liberar el material refráctil; y iii) llevar a cabo una centrifugación a baja velocidad para formar un nódulo de material refráctil. Un método para reactivar una proteína formada como un producto de expresión heterólogo en una célula huésped, el cual comprende tratar una solución débilmente desnaturalizante de la proteína con un compuesto sulfhídrilo, en presencia de una cantidad más pequeña de su disulfuro correspondiente.
MX199852A 1982-12-22 1983-12-21 Metodos de purificacion y reactivacion de proteinas heterologas precipitadas MX166885B (es)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US45235582A 1982-12-22 1982-12-22
US45225282A 1982-12-22 1982-12-22
US45235682A 1982-12-22 1982-12-22
US45218782A 1982-12-22 1982-12-22
US45234482A 1982-12-22 1982-12-22
US45236382A 1982-12-22 1982-12-22
US45235782A 1982-12-22 1982-12-22
US45225382A 1982-12-22 1982-12-22

Publications (1)

Publication Number Publication Date
MX166885B true MX166885B (es) 1993-02-10

Family

ID=27575443

Family Applications (1)

Application Number Title Priority Date Filing Date
MX199852A MX166885B (es) 1982-12-22 1983-12-21 Metodos de purificacion y reactivacion de proteinas heterologas precipitadas

Country Status (12)

Country Link
EP (1) EP0114506B1 (es)
JP (1) JP2575604B2 (es)
AU (1) AU568109B2 (es)
BR (1) BR8307086A (es)
CA (1) CA1219234A (es)
DE (1) DE3380850D1 (es)
GR (1) GR79124B (es)
HU (1) HU197357B (es)
IL (1) IL70474A (es)
MX (1) MX166885B (es)
NZ (1) NZ206615A (es)
PT (1) PT77866B (es)

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Also Published As

Publication number Publication date
AU2259483A (en) 1984-06-28
JPS59161321A (ja) 1984-09-12
JP2575604B2 (ja) 1997-01-29
EP0114506A1 (en) 1984-08-01
PT77866B (en) 1986-04-09
GR79124B (es) 1984-10-02
HU197357B (en) 1989-03-28
AU568109B2 (en) 1987-12-17
PT77866A (en) 1984-01-01
IL70474A (en) 1989-09-28
EP0114506B1 (en) 1989-11-15
IL70474A0 (en) 1984-03-30
DE3380850D1 (en) 1989-12-21
CA1219234A (en) 1987-03-17
BR8307086A (pt) 1985-02-12
NZ206615A (en) 1988-03-30

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