ES2059367T3 - Produccion de proteinas mediante cultivo de celulas. - Google Patents

Produccion de proteinas mediante cultivo de celulas.

Info

Publication number
ES2059367T3
ES2059367T3 ES87302156T ES87302156T ES2059367T3 ES 2059367 T3 ES2059367 T3 ES 2059367T3 ES 87302156 T ES87302156 T ES 87302156T ES 87302156 T ES87302156 T ES 87302156T ES 2059367 T3 ES2059367 T3 ES 2059367T3
Authority
ES
Spain
Prior art keywords
proteins
agents
cell culture
protein production
enhance production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87302156T
Other languages
English (en)
Inventor
Raymond Paul Field
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UCB Celltech Ltd
Original Assignee
Celltech R&D Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10594635&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2059367(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Celltech R&D Ltd filed Critical Celltech R&D Ltd
Application granted granted Critical
Publication of ES2059367T3 publication Critical patent/ES2059367T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE OBTIENEN RENDIMIENTOS AUMENTADOS MEDIANTE CULTIVO POR INGENIERIA GENETICA DE CELULAS DE HIBRIDOMA QUE CONSTITUTIVAMENTE PRODUCEN LAS PROTEINAS, EN PRESENCIA DE AGENTES QUIMICOS QUE AUMENTAN LA PRODUCCION DE PROTEINAS. LOS AGENTES SE EMPLEAN A CONCENTRACIONES QUE AUMENTAN LA PRODUCCION DE LAS PROTEINAS PERO NO DISMINUYEN SUSTANCIALMENTE LA VELOCIDAD DE CRECIMIENTO CELULAR NI REDUCEN SIGNIFICATIVAMENTE LA VIABILIDAD DE LAS CELULAS. LOS AGENTES INCLUYEN ACIDOS ALCANOICOS Y SUS SALES. LAS PROTEINAS INCLUYEN PROTEINAS RECOMBINANTES E INMUNOGLOBULINAS.
ES87302156T 1986-03-14 1987-03-13 Produccion de proteinas mediante cultivo de celulas. Expired - Lifetime ES2059367T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB868606386A GB8606386D0 (en) 1986-03-14 1986-03-14 Production of protein

Publications (1)

Publication Number Publication Date
ES2059367T3 true ES2059367T3 (es) 1994-11-16

Family

ID=10594635

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87302156T Expired - Lifetime ES2059367T3 (es) 1986-03-14 1987-03-13 Produccion de proteinas mediante cultivo de celulas.

Country Status (9)

Country Link
EP (1) EP0239292B1 (es)
JP (1) JP2525436B2 (es)
AT (1) ATE95231T1 (es)
AU (1) AU7126487A (es)
CA (1) CA1341367C (es)
DE (1) DE3787551T2 (es)
ES (1) ES2059367T3 (es)
GB (2) GB8606386D0 (es)
WO (1) WO1987005626A1 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU619226B2 (en) * 1987-09-28 1992-01-23 Children's Hospital Medical Center Human cell lines of epithelial lung adenocarcinoma origin, human proteins and methods
ES2064336T3 (es) * 1987-10-13 1995-02-01 Thomae Gmbh Dr K Procedimiento para la preparacion de proteinas.
AU2943389A (en) * 1988-01-13 1989-08-11 Upjohn Company, The Enhanced production of cellular proteins using butyrate
FR2635008B1 (fr) * 1988-08-05 1990-11-16 Sanofi Sa Agent activateur de la productivite specifique des cellules animales recombinantes a base de polyvinylpyrrolidone et milieu de culture defini le contenant
US5378612A (en) * 1990-05-11 1995-01-03 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Culture medium for production of recombinant protein
FR2674053A1 (fr) * 1991-03-12 1992-09-18 Dassault Electronique Installation pour la securisation de titres de transport, en particulier a piste magnetique.
IL102189A (en) * 1992-06-12 1995-07-31 Univ Ben Gurion Device for growing microorganisms
US5705364A (en) * 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
US6656466B1 (en) 1995-06-06 2003-12-02 Genetech, Inc. Human tumor necrosis factor—immunoglobulin(TNFR1-IgG1) chimera composition
US5721121A (en) * 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
KR100447422B1 (ko) * 1996-11-28 2004-10-28 씨제이 주식회사 포유동물세포유래성당단백질의당쇄화및생산성향상성을위한새로운세포배양배지
GB9828624D0 (en) 1998-12-23 1999-02-17 Glaxo Group Ltd Production of proteins
KR100397791B1 (ko) * 2000-08-30 2003-09-13 한국과학기술원 ＣＨＯ세포에서 ｂｃｌ-2 유전자 과발현 및 배지내 소디움부티레이트 첨가에 의한 인간화된 항체의 생산성을증가시키는 방법
KR101007171B1 (ko) * 2002-04-08 2011-01-12 론자 바이올로직스 피엘씨 세포 배양 배지
GB0208041D0 (en) * 2002-04-08 2002-05-22 Lonza Biologics Plc Method of culturing animal cells
WO2004009782A2 (en) * 2002-07-19 2004-01-29 Morphotek, Inc. Methods for generating enhanced antibody-producing cell lines with improved growth characteristics
EP1620457A2 (en) * 2003-04-25 2006-02-01 Immunex Corporation Inducers of recombinant protein expression
WO2005064002A1 (en) * 2003-12-31 2005-07-14 Samyang Genex Corporation Method for mass production of secondary metabolites in plant cell culture by treatment of an alkanoic acid or salt thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5774093A (en) * 1980-10-27 1982-05-10 Ajinomoto Co Inc Method of increasing interferon production

Also Published As

Publication number Publication date
CA1341367C (en) 2002-06-11
GB8726077D0 (en) 1987-12-09
GB2196636B (en) 1990-09-19
GB8606386D0 (en) 1986-04-23
DE3787551D1 (de) 1993-11-04
EP0239292B1 (en) 1993-09-29
EP0239292A1 (en) 1987-09-30
JPS63503273A (ja) 1988-12-02
WO1987005626A1 (en) 1987-09-24
ATE95231T1 (de) 1993-10-15
GB2196636A (es) 1988-05-05
DE3787551T2 (de) 1994-02-17
JP2525436B2 (ja) 1996-08-21
AU7126487A (en) 1987-10-09

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