JPS6443185A - Culture medium for propagation - Google Patents
Culture medium for propagationInfo
- Publication number
- JPS6443185A JPS6443185A JP20232087A JP20232087A JPS6443185A JP S6443185 A JPS6443185 A JP S6443185A JP 20232087 A JP20232087 A JP 20232087A JP 20232087 A JP20232087 A JP 20232087A JP S6443185 A JPS6443185 A JP S6443185A
- Authority
- JP
- Japan
- Prior art keywords
- culture medium
- iron
- manganese
- compound
- propagation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE:To enhance bacterium cell concentration of Escherichia coli and improve the productivity of useful protein, by cultivating a recombinant Escherichia coli in a culture medium containing an iron compound or manganese compound. CONSTITUTION:Escherichia coli retaining an useful protein expressing plasmid and transformed is cultivated in a culture medium obtained by adding 1muM-10mM, preferably 3-100muM bivalent iron compound such as iron (II) chloride or iron (II) sulfate and/or 1muM-10mM, preferably 5muM-100muM bivalent manganese compound such as manganese chloride or manganese sulfate to a culture medium for propagation such as LB culture medium containing carbon source, nitrogen source, inorganic salts, etc. and the culture medium is replaced by a producing culture medium limited in an addition amount of iron or manganese and added by 3XM-1mM, preferably 5-500muM zinc compound to provide also useful protein such as human beta type interferon. Cultivation by culture medium for propagation and cultivation for producing culture medium are preferably carried out at 5-8pH and 20-37 deg.C for 1-4 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20232087A JPS6443185A (en) | 1987-08-12 | 1987-08-12 | Culture medium for propagation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20232087A JPS6443185A (en) | 1987-08-12 | 1987-08-12 | Culture medium for propagation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6443185A true JPS6443185A (en) | 1989-02-15 |
Family
ID=16455594
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20232087A Pending JPS6443185A (en) | 1987-08-12 | 1987-08-12 | Culture medium for propagation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6443185A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62175191A (en) * | 1985-04-30 | 1987-07-31 | Takeda Chem Ind Ltd | Yield increase of protein |
-
1987
- 1987-08-12 JP JP20232087A patent/JPS6443185A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62175191A (en) * | 1985-04-30 | 1987-07-31 | Takeda Chem Ind Ltd | Yield increase of protein |
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