AU2373388A - Method for preparing proteins - Google Patents

Method for preparing proteins

Info

Publication number
AU2373388A
AU2373388A AU23733/88A AU2373388A AU2373388A AU 2373388 A AU2373388 A AU 2373388A AU 23733/88 A AU23733/88 A AU 23733/88A AU 2373388 A AU2373388 A AU 2373388A AU 2373388 A AU2373388 A AU 2373388A
Authority
AU
Australia
Prior art keywords
acid
protein
anhydromevalonolactone
hydroxy
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU23733/88A
Other versions
AU614999B2 (en
Inventor
Andrea Stecher-Schilling
Rolf-Gunter Werner
William Werz
Hans Zahner
Axel Zeeck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH and Co KG
Original Assignee
Dr Karl Thomae GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19873734632 external-priority patent/DE3734632A1/en
Priority claimed from DE19873738649 external-priority patent/DE3738649A1/en
Priority claimed from DE19883801562 external-priority patent/DE3801562A1/en
Application filed by Dr Karl Thomae GmbH filed Critical Dr Karl Thomae GmbH
Publication of AU2373388A publication Critical patent/AU2373388A/en
Application granted granted Critical
Publication of AU614999B2 publication Critical patent/AU614999B2/en
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A method for increasing the production of protein, especially t-PA, in cultures of cells producing this protein, where a substance which induces the production of the protein is added to the cell culture, is characterised in that thioglycolic acid, thiodiglycolic acid, L-cysteine, glutathione, butyrylcholine bromide, butyrylcholine chloride, nonactic acid, furan fatty acid, ascorbic acid, aphidicolin, 6-hydroxy-4,6-dimethyl-3-hepten-2-one, fusaric acid, mevalonic acid, trans-anhydromevalonic acid, anhydromevalonolactone, cis-anhydromevalonolactone, D- alpha -hydroxy-substituted (C3 or C4) aliphatic mono- or dicarboxylic acid or the salts thereof is used as protein-inducing substance.
AU23733/88A 1987-10-13 1988-10-12 Method for preparing proteins Expired AU614999B2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE3734632 1987-10-13
DE19873734632 DE3734632A1 (en) 1987-10-13 1987-10-13 Process for the preparation of t-PA
DE3738649 1987-11-13
DE19873738649 DE3738649A1 (en) 1987-11-13 1987-11-13 Process for the preparation of proteins
DE3801562 1988-01-20
DE19883801562 DE3801562A1 (en) 1988-01-20 1988-01-20 Process for the preparation of proteins

Publications (2)

Publication Number Publication Date
AU2373388A true AU2373388A (en) 1989-05-11
AU614999B2 AU614999B2 (en) 1991-09-19

Family

ID=27196629

Family Applications (1)

Application Number Title Priority Date Filing Date
AU23733/88A Expired AU614999B2 (en) 1987-10-13 1988-10-12 Method for preparing proteins

Country Status (16)

Country Link
EP (1) EP0315782B1 (en)
JP (1) JP2688222B2 (en)
KR (1) KR890006671A (en)
AT (1) ATE112308T1 (en)
AU (1) AU614999B2 (en)
CA (1) CA1341400C (en)
DE (1) DE3851685D1 (en)
DK (1) DK175411B1 (en)
ES (1) ES2064336T3 (en)
FI (1) FI94963C (en)
HU (1) HU205381B (en)
IE (1) IE65986B1 (en)
IL (1) IL88001A (en)
NO (1) NO174778C (en)
NZ (1) NZ226521A (en)
PT (1) PT88751B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE210150T1 (en) * 1991-04-18 2001-12-15 Toray Industries METHOD FOR PRODUCING INTERLEUKIN-6 COMPOSITIONS
DE4113750A1 (en) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh IMPROVEMENT OF RENATURATION IN THE SECRETION OF DISULFID-BRIDGED PROTEINS
CA2286395C (en) * 1997-04-03 2009-06-23 Yoshitomi Pharmaceutical Industries Ltd. Production method of heterologous protein
AU2002354363A1 (en) * 2001-12-04 2003-06-17 Mitsubishi Pharma Corporation Method of activating protein
JPWO2004056872A1 (en) * 2002-12-20 2006-04-20 三菱ウェルファーマ株式会社 Method for protecting thiol groups of proteins
WO2008136398A1 (en) 2007-04-26 2008-11-13 Chugai Seiyaku Kabushiki Kaisha Cell culture method using amino acid-enriched medium

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3479814D1 (en) * 1984-06-05 1989-10-26 Asahi Chemical Ind Process for the preparation of a plasminogen activator
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein

Also Published As

Publication number Publication date
NO884549D0 (en) 1988-10-12
FI884652A (en) 1989-04-14
HU205381B (en) 1992-04-28
DE3851685D1 (en) 1994-11-03
HUT48306A (en) 1989-05-29
DK567888A (en) 1989-04-14
IE883079L (en) 1989-04-13
DK567888D0 (en) 1988-10-12
EP0315782B1 (en) 1994-09-28
IL88001A0 (en) 1989-06-30
IL88001A (en) 1996-01-19
PT88751B (en) 1995-03-01
NZ226521A (en) 1991-04-26
EP0315782A3 (en) 1989-09-20
PT88751A (en) 1989-07-31
EP0315782A2 (en) 1989-05-17
AU614999B2 (en) 1991-09-19
ATE112308T1 (en) 1994-10-15
DK175411B1 (en) 2004-09-27
FI884652A0 (en) 1988-10-11
CA1341400C (en) 2002-11-19
FI94963C (en) 1995-11-27
NO174778B (en) 1994-03-28
ES2064336T3 (en) 1995-02-01
JPH01231887A (en) 1989-09-18
JP2688222B2 (en) 1997-12-08
NO174778C (en) 1994-07-06
IE65986B1 (en) 1995-11-29
FI94963B (en) 1995-08-15
NO884549L (en) 1989-04-14
KR890006671A (en) 1989-06-15

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