IL88001A - Method for increasing the production of recombinant proteins produced by cho cells in culture - Google Patents

Method for increasing the production of recombinant proteins produced by cho cells in culture

Info

Publication number
IL88001A
IL88001A IL8800188A IL8800188A IL88001A IL 88001 A IL88001 A IL 88001A IL 8800188 A IL8800188 A IL 8800188A IL 8800188 A IL8800188 A IL 8800188A IL 88001 A IL88001 A IL 88001A
Authority
IL
Israel
Prior art keywords
acid
production
protein
increasing
culture
Prior art date
Application number
IL8800188A
Other versions
IL88001A0 (en
Original Assignee
Thomae Gmbh Dr K
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19873734632 external-priority patent/DE3734632A1/en
Priority claimed from DE19873738649 external-priority patent/DE3738649A1/en
Priority claimed from DE19883801562 external-priority patent/DE3801562A1/en
Application filed by Thomae Gmbh Dr K filed Critical Thomae Gmbh Dr K
Publication of IL88001A0 publication Critical patent/IL88001A0/en
Publication of IL88001A publication Critical patent/IL88001A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A method for increasing the production of protein, especially t-PA, in cultures of cells producing this protein, where a substance which induces the production of the protein is added to the cell culture, is characterised in that thioglycolic acid, thiodiglycolic acid, L-cysteine, glutathione, butyrylcholine bromide, butyrylcholine chloride, nonactic acid, furan fatty acid, ascorbic acid, aphidicolin, 6-hydroxy-4,6-dimethyl-3-hepten-2-one, fusaric acid, mevalonic acid, trans-anhydromevalonic acid, anhydromevalonolactone, cis-anhydromevalonolactone, D- alpha -hydroxy-substituted (C3 or C4) aliphatic mono- or dicarboxylic acid or the salts thereof is used as protein-inducing substance.
IL8800188A 1987-10-13 1988-10-11 Method for increasing the production of recombinant proteins produced by cho cells in culture IL88001A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19873734632 DE3734632A1 (en) 1987-10-13 1987-10-13 Process for the preparation of t-PA
DE19873738649 DE3738649A1 (en) 1987-11-13 1987-11-13 Process for the preparation of proteins
DE19883801562 DE3801562A1 (en) 1988-01-20 1988-01-20 Process for the preparation of proteins

Publications (2)

Publication Number Publication Date
IL88001A0 IL88001A0 (en) 1989-06-30
IL88001A true IL88001A (en) 1996-01-19

Family

ID=27196629

Family Applications (1)

Application Number Title Priority Date Filing Date
IL8800188A IL88001A (en) 1987-10-13 1988-10-11 Method for increasing the production of recombinant proteins produced by cho cells in culture

Country Status (16)

Country Link
EP (1) EP0315782B1 (en)
JP (1) JP2688222B2 (en)
KR (1) KR890006671A (en)
AT (1) ATE112308T1 (en)
AU (1) AU614999B2 (en)
CA (1) CA1341400C (en)
DE (1) DE3851685D1 (en)
DK (1) DK175411B1 (en)
ES (1) ES2064336T3 (en)
FI (1) FI94963C (en)
HU (1) HU205381B (en)
IE (1) IE65986B1 (en)
IL (1) IL88001A (en)
NO (1) NO174778C (en)
NZ (1) NZ226521A (en)
PT (1) PT88751B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2164050T3 (en) * 1991-04-18 2002-02-16 Toray Industries PREPARATION OF INTERLEUQUINE COMPOUNDS-6.
DE4113750A1 (en) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh IMPROVEMENT OF RENATURATION IN THE SECRETION OF DISULFID-BRIDGED PROTEINS
CN1257549A (en) * 1997-04-03 2000-06-21 吉富制药株式会社 Processf or production foreign proteins
EP1452597A4 (en) * 2001-12-04 2006-09-20 Mitsubishi Pharma Corp Method of activating protein
CA2511228A1 (en) * 2002-12-20 2004-07-08 Mitsubishi Pharma Corporation Method of protecting thiol group of protein
DK2154244T3 (en) * 2007-04-26 2017-06-12 Chugai Pharmaceutical Co Ltd CELL CULTIVATION PROCEDURE WHEN AN ACID-ENRICHED MEDIUM IS USED

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0163751B1 (en) * 1984-06-05 1989-09-20 Asahi Kasei Kogyo Kabushiki Kaisha Process for the preparation of a plasminogen activator
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein

Also Published As

Publication number Publication date
NO884549D0 (en) 1988-10-12
HU205381B (en) 1992-04-28
KR890006671A (en) 1989-06-15
NZ226521A (en) 1991-04-26
HUT48306A (en) 1989-05-29
PT88751B (en) 1995-03-01
PT88751A (en) 1989-07-31
FI94963C (en) 1995-11-27
JP2688222B2 (en) 1997-12-08
ES2064336T3 (en) 1995-02-01
NO174778C (en) 1994-07-06
ATE112308T1 (en) 1994-10-15
JPH01231887A (en) 1989-09-18
NO884549L (en) 1989-04-14
DK567888A (en) 1989-04-14
IE883079L (en) 1989-04-13
DE3851685D1 (en) 1994-11-03
CA1341400C (en) 2002-11-19
FI884652A0 (en) 1988-10-11
EP0315782B1 (en) 1994-09-28
AU2373388A (en) 1989-05-11
FI94963B (en) 1995-08-15
IE65986B1 (en) 1995-11-29
AU614999B2 (en) 1991-09-19
NO174778B (en) 1994-03-28
FI884652A (en) 1989-04-14
IL88001A0 (en) 1989-06-30
DK567888D0 (en) 1988-10-12
DK175411B1 (en) 2004-09-27
EP0315782A2 (en) 1989-05-17
EP0315782A3 (en) 1989-09-20

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