FI884652A - A method for enhancing the production of t-PA-like proteins - Google Patents

A method for enhancing the production of t-PA-like proteins Download PDF

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Publication number
FI884652A
FI884652A FI884652A FI884652A FI884652A FI 884652 A FI884652 A FI 884652A FI 884652 A FI884652 A FI 884652A FI 884652 A FI884652 A FI 884652A FI 884652 A FI884652 A FI 884652A
Authority
FI
Finland
Prior art keywords
acid
protein
production
anhydromevalonolactone
hydroxy
Prior art date
Application number
FI884652A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI94963C (en
FI884652A0 (en
FI94963B (en
Inventor
Andrea Stecher-Schilling
Rolf-Guenter Werner
William Werz
Hans Zaehner
Axel Zeeck
Original Assignee
Thomae Gmbh Dr K
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19873734632 external-priority patent/DE3734632A1/en
Priority claimed from DE19873738649 external-priority patent/DE3738649A1/en
Priority claimed from DE19883801562 external-priority patent/DE3801562A1/en
Application filed by Thomae Gmbh Dr K filed Critical Thomae Gmbh Dr K
Publication of FI884652A0 publication Critical patent/FI884652A0/en
Publication of FI884652A publication Critical patent/FI884652A/en
Application granted granted Critical
Publication of FI94963B publication Critical patent/FI94963B/en
Publication of FI94963C publication Critical patent/FI94963C/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A method for increasing the production of protein, especially t-PA, in cultures of cells producing this protein, where a substance which induces the production of the protein is added to the cell culture, is characterised in that thioglycolic acid, thiodiglycolic acid, L-cysteine, glutathione, butyrylcholine bromide, butyrylcholine chloride, nonactic acid, furan fatty acid, ascorbic acid, aphidicolin, 6-hydroxy-4,6-dimethyl-3-hepten-2-one, fusaric acid, mevalonic acid, trans-anhydromevalonic acid, anhydromevalonolactone, cis-anhydromevalonolactone, D- alpha -hydroxy-substituted (C3 or C4) aliphatic mono- or dicarboxylic acid or the salts thereof is used as protein-inducing substance.
FI884652A 1987-10-13 1988-10-11 Process for streamlining the production of t-PA-like proteins FI94963C (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE3734632 1987-10-13
DE19873734632 DE3734632A1 (en) 1987-10-13 1987-10-13 Process for the preparation of t-PA
DE3738649 1987-11-13
DE19873738649 DE3738649A1 (en) 1987-11-13 1987-11-13 Process for the preparation of proteins
DE19883801562 DE3801562A1 (en) 1988-01-20 1988-01-20 Process for the preparation of proteins
DE3801562 1988-01-20

Publications (4)

Publication Number Publication Date
FI884652A0 FI884652A0 (en) 1988-10-11
FI884652A true FI884652A (en) 1989-04-14
FI94963B FI94963B (en) 1995-08-15
FI94963C FI94963C (en) 1995-11-27

Family

ID=27196629

Family Applications (1)

Application Number Title Priority Date Filing Date
FI884652A FI94963C (en) 1987-10-13 1988-10-11 Process for streamlining the production of t-PA-like proteins

Country Status (16)

Country Link
EP (1) EP0315782B1 (en)
JP (1) JP2688222B2 (en)
KR (1) KR890006671A (en)
AT (1) ATE112308T1 (en)
AU (1) AU614999B2 (en)
CA (1) CA1341400C (en)
DE (1) DE3851685D1 (en)
DK (1) DK175411B1 (en)
ES (1) ES2064336T3 (en)
FI (1) FI94963C (en)
HU (1) HU205381B (en)
IE (1) IE65986B1 (en)
IL (1) IL88001A (en)
NO (1) NO174778C (en)
NZ (1) NZ226521A (en)
PT (1) PT88751B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2164050T3 (en) * 1991-04-18 2002-02-16 Toray Industries PREPARATION OF INTERLEUQUINE COMPOUNDS-6.
DE4113750A1 (en) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh IMPROVEMENT OF RENATURATION IN THE SECRETION OF DISULFID-BRIDGED PROTEINS
CN1257549A (en) * 1997-04-03 2000-06-21 吉富制药株式会社 Processf or production foreign proteins
EP1452597A4 (en) * 2001-12-04 2006-09-20 Mitsubishi Pharma Corp Method of activating protein
CA2511228A1 (en) * 2002-12-20 2004-07-08 Mitsubishi Pharma Corporation Method of protecting thiol group of protein
DK2154244T3 (en) * 2007-04-26 2017-06-12 Chugai Pharmaceutical Co Ltd CELL CULTIVATION PROCEDURE WHEN AN ACID-ENRICHED MEDIUM IS USED

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0163751B1 (en) * 1984-06-05 1989-09-20 Asahi Kasei Kogyo Kabushiki Kaisha Process for the preparation of a plasminogen activator
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein

Also Published As

Publication number Publication date
NO884549D0 (en) 1988-10-12
HU205381B (en) 1992-04-28
KR890006671A (en) 1989-06-15
NZ226521A (en) 1991-04-26
HUT48306A (en) 1989-05-29
IL88001A (en) 1996-01-19
PT88751B (en) 1995-03-01
PT88751A (en) 1989-07-31
FI94963C (en) 1995-11-27
JP2688222B2 (en) 1997-12-08
ES2064336T3 (en) 1995-02-01
NO174778C (en) 1994-07-06
ATE112308T1 (en) 1994-10-15
JPH01231887A (en) 1989-09-18
NO884549L (en) 1989-04-14
DK567888A (en) 1989-04-14
IE883079L (en) 1989-04-13
DE3851685D1 (en) 1994-11-03
CA1341400C (en) 2002-11-19
FI884652A0 (en) 1988-10-11
EP0315782B1 (en) 1994-09-28
AU2373388A (en) 1989-05-11
FI94963B (en) 1995-08-15
IE65986B1 (en) 1995-11-29
AU614999B2 (en) 1991-09-19
NO174778B (en) 1994-03-28
IL88001A0 (en) 1989-06-30
DK567888D0 (en) 1988-10-12
DK175411B1 (en) 2004-09-27
EP0315782A2 (en) 1989-05-17
EP0315782A3 (en) 1989-09-20

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BB Publication of examined application
FG Patent granted

Owner name: DR. KARL THOMAE GESELLSCHAFT MIT

MA Patent expired