ES2064336T3 - Procedimiento para la preparacion de proteinas. - Google Patents

Procedimiento para la preparacion de proteinas.

Info

Publication number
ES2064336T3
ES2064336T3 ES88116739T ES88116739T ES2064336T3 ES 2064336 T3 ES2064336 T3 ES 2064336T3 ES 88116739 T ES88116739 T ES 88116739T ES 88116739 T ES88116739 T ES 88116739T ES 2064336 T3 ES2064336 T3 ES 2064336T3
Authority
ES
Spain
Prior art keywords
acid
protein
anhydromevalonolactone
hydroxy
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88116739T
Other languages
English (en)
Inventor
Andrea Dr Di Stecher-Schilling
Rolf-Gunter Dr Dipl-Mik Werner
William Dr Dipl-Biologe Werz
Hans Prof Dr Zahner
Axel Prof Dr Dipl-Chem Zeeck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH and Co KG
Original Assignee
Dr Karl Thomae GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19873734632 external-priority patent/DE3734632A1/de
Priority claimed from DE19873738649 external-priority patent/DE3738649A1/de
Priority claimed from DE19883801562 external-priority patent/DE3801562A1/de
Application filed by Dr Karl Thomae GmbH filed Critical Dr Karl Thomae GmbH
Application granted granted Critical
Publication of ES2064336T3 publication Critical patent/ES2064336T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

METODO PARA LA ELEVACION DE LA PRODUCCION DE PROTEINA, ESPECIALMENTE DE T-PA EN CULTIVOS DE CELULAS PRODUCTORAS DE ESTA PROTEINA, EN EL QUE SE AÑADE AL CULTIVO DE CALULAS UNA SUSTANCIA INDUCTORA DE LA PRODUCCION DE PROTEINAS, CARACTERIZADO POR EL USO COMO INDUCTOR DE PROTEINAS DE ACIDO DE TIOGLICOL, ACIDO DE TIODIGLICOL, L-CISTEINA, GLUTATION, BROMURO DE BUTIRILCOLINA, CLORURO DE BUTIRILCOLINA, ACIDO NONACTINICO, ACIDO FURANOGRASO, ACIDO ASCORBICO, AFIDICOLINA, 6-HIDROXI-4,6-DIMETIL-3-HEPTEN-2-ONA, ACIDO FUSARINICO, ACIDO MEVALONICO, ACIDO DE TRANS-ANHIDROMEVALONA, LACTONA DEL ACIDO ANHIDROMEVALONICO, LACTONA DEL ACIDO CISANHIDROMEVALONICO, ACIDO MONO- O DICARBOXILICO D-(ALFA)-HIDROXISUSTITUIDO (C3 O C4) ALIFATICO O SUS SALES.
ES88116739T 1987-10-13 1988-10-10 Procedimiento para la preparacion de proteinas. Expired - Lifetime ES2064336T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19873734632 DE3734632A1 (de) 1987-10-13 1987-10-13 Verfahren zur herstellung von t-pa
DE19873738649 DE3738649A1 (de) 1987-11-13 1987-11-13 Verfahren zur herstellung von proteinen
DE19883801562 DE3801562A1 (de) 1988-01-20 1988-01-20 Verfahren zur herstellung von proteinen

Publications (1)

Publication Number Publication Date
ES2064336T3 true ES2064336T3 (es) 1995-02-01

Family

ID=27196629

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88116739T Expired - Lifetime ES2064336T3 (es) 1987-10-13 1988-10-10 Procedimiento para la preparacion de proteinas.

Country Status (16)

Country Link
EP (1) EP0315782B1 (es)
JP (1) JP2688222B2 (es)
KR (1) KR890006671A (es)
AT (1) ATE112308T1 (es)
AU (1) AU614999B2 (es)
CA (1) CA1341400C (es)
DE (1) DE3851685D1 (es)
DK (1) DK175411B1 (es)
ES (1) ES2064336T3 (es)
FI (1) FI94963C (es)
HU (1) HU205381B (es)
IE (1) IE65986B1 (es)
IL (1) IL88001A (es)
NO (1) NO174778C (es)
NZ (1) NZ226521A (es)
PT (1) PT88751B (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2164050T3 (es) * 1991-04-18 2002-02-16 Toray Industries Preparacion de compuestos de interleuquina-6.
DE4113750A1 (de) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
CN1257549A (zh) * 1997-04-03 2000-06-21 吉富制药株式会社 异源蛋白的生产方法
EP1452597A4 (en) * 2001-12-04 2006-09-20 Mitsubishi Pharma Corp PROTEIN ACTIVATION METHOD
CA2511228A1 (en) * 2002-12-20 2004-07-08 Mitsubishi Pharma Corporation Method of protecting thiol group of protein
DK2154244T3 (en) * 2007-04-26 2017-06-12 Chugai Pharmaceutical Co Ltd CELL CULTIVATION PROCEDURE WHEN AN ACID-ENRICHED MEDIUM IS USED

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0163751B1 (en) * 1984-06-05 1989-09-20 Asahi Kasei Kogyo Kabushiki Kaisha Process for the preparation of a plasminogen activator
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein

Also Published As

Publication number Publication date
NO884549D0 (no) 1988-10-12
HU205381B (en) 1992-04-28
KR890006671A (ko) 1989-06-15
NZ226521A (en) 1991-04-26
HUT48306A (en) 1989-05-29
IL88001A (en) 1996-01-19
PT88751B (pt) 1995-03-01
PT88751A (pt) 1989-07-31
FI94963C (fi) 1995-11-27
JP2688222B2 (ja) 1997-12-08
NO174778C (no) 1994-07-06
ATE112308T1 (de) 1994-10-15
JPH01231887A (ja) 1989-09-18
NO884549L (no) 1989-04-14
DK567888A (da) 1989-04-14
IE883079L (en) 1989-04-13
DE3851685D1 (de) 1994-11-03
CA1341400C (en) 2002-11-19
FI884652A0 (fi) 1988-10-11
EP0315782B1 (de) 1994-09-28
AU2373388A (en) 1989-05-11
FI94963B (fi) 1995-08-15
IE65986B1 (en) 1995-11-29
AU614999B2 (en) 1991-09-19
NO174778B (no) 1994-03-28
FI884652A (fi) 1989-04-14
IL88001A0 (en) 1989-06-30
DK567888D0 (da) 1988-10-12
DK175411B1 (da) 2004-09-27
EP0315782A2 (de) 1989-05-17
EP0315782A3 (en) 1989-09-20

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