KR890006671A - 단백질 제조방법 - Google Patents

단백질 제조방법 Download PDF

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KR890006671A
KR890006671A KR1019880013334A KR880013334A KR890006671A KR 890006671 A KR890006671 A KR 890006671A KR 1019880013334 A KR1019880013334 A KR 1019880013334A KR 880013334 A KR880013334 A KR 880013334A KR 890006671 A KR890006671 A KR 890006671A
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South Korea
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acid
protein
salt
culture
added
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KR1019880013334A
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English (en)
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스테헤르-쉴링 안드레아
베르너 롤프-귄터
베르쯔 윌리암
제너 한스
지이크 악셀
Original Assignee
디이터 라우디엔, 게르하르트 후버
닥터 칼 토매 지엠비에이치
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Priority claimed from DE19873734632 external-priority patent/DE3734632A1/de
Priority claimed from DE19873738649 external-priority patent/DE3738649A1/de
Priority claimed from DE19883801562 external-priority patent/DE3801562A1/de
Application filed by 디이터 라우디엔, 게르하르트 후버, 닥터 칼 토매 지엠비에이치 filed Critical 디이터 라우디엔, 게르하르트 후버
Publication of KR890006671A publication Critical patent/KR890006671A/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음

Description

단백질 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 7.5% FCS를 함유하는 배지중에서 배양된 CHO 세포의 세포생장을 나타낸다.

Claims (17)

  1. 세포 배양액에 단백질 유도물질로서 티오글리콜산, 티오디글리콜산, L-시스테인, 글루타티온, 부티릴콜린 브로마이드, 부티릴콜린 클로라이드, 노낙트산, 푸란 지방산, 아스코르브산, 아피디콜린, 6-하이드록시-4,6-디메틸-3-헵텐-2-온, 푸자르산, 메발론산, 프란스-무수 메발론산, 무수 메발론산 락톤, 시스-무수 메발론산 락톤, D-α-하이드록시-치환된(C3또는 C4) 지방족 모노-또는 디카복실산 또는 이의 염을 가함을 특징으로 하여, 단백질을 생하는 세포 배양시 단백질의 생산을 증가시키는 방법.
  2. C3-C4지방족 모노카복실산 또는 이의 염을 배양액에 가함을 특징으로 하여, 형질전환된 CHO세포의 배양액으로부터 단백질의 생산을 증가시키는 방법.
  3. 제2항에 있어서, 단백질이 t-PA 또는 이의 돌연변이체임을 특징으로 하는 방법.
  4. 제1항에 있어서, 형질전환된 CHO 세포를 배양함을 특징으로 하는 방법.
  5. 제4항에 있어서, 단백질이 t-PA 또는 이의 돌연변이체임을 특징으로 하는 방법.
  6. 제5항에 있어서, 티오글리콜산 또는 이의 염을 단백질-유도물질로서 사용함을 특징으로 하는 방법.
  7. 제5항에 있어서, 노낙트산 또는 푸란 지방산 또는 이의 염을 단백질-유도 물질로서 사용함을 특징으로 하는 방법.
  8. 제2항 또는 제3항에 있어서, 부티르산 또는 이의 염을 단백질-유도물질로서 사용함을 특징으로 하는 방법.
  9. 제1항 내지 제8항중 어느 한 항에 있어서, 혈청을 함유하지 않은 배지중에서 배양시킴을 특징으로 하는 방법.
  10. 제1항 내지 제8항중 어느 한 항에 있어서, 혈청을 함유하는 배지중에서 배양시킴을 특징으로 하는 방법.
  11. 제1항 내지 제10항중 어느 한 항에 있어서, 배양액중에 단백질 유도물질을 0.005mcM 내지 500mM의 농도로 가함을 특징으로 하는 방법.
  12. 제11항에 있어서, C3-4지방족 모노카복실산 또는 이의 염을 1mM 내지 10mM의 농도로 가함을 특징으로 하는 방법.
  13. 제11항에 있어서, 티오글리콜산, 티오디글리콜산, L-시스테인 또는 글루타티온 또는 이의 염을 1mM 내지 10mM 의 농도로 가함을 특징으로 하는 방법.
  14. 제1항 내지 제13항중 어느 한 항에 있어서, 세포를 상기 단백질 유도물질중 어느 하나의 존재하에 배양시키고, 세포를 배양액으로부터 분리하여 새로운 배양배지상에 넣은 다음 상기 단백질 유도물질중 어느 하나의 존재하에 배양시킴을 특징으로 하는 방법.
  15. 제14항에 있어서, 아피디콜린이 첫번째 언급된 단백질 유도물질이고 두번째 물질이 브티르산, 프로피온산, 부틸릴 콜린 클로라이드 또는 부티릴콜린 브롬 또는 이의 염임을 특징으로 하는 방법.
  16. 제1항 내지 제13항중 어느 한 항에 있어서, 두개 또는 그 이상의 물질을 동시에 사용함을 특징으로 하는 방법.
  17. 제16항에 있어서, 부티르산 및 티오글리콜산 또는 이의 염을 사용하며, 두번째 물질로서 부티르산을 세포배양액에 가함을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880013334A 1987-10-13 1988-10-13 단백질 제조방법 KR890006671A (ko)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE?P3734632.6? 1987-10-13
DE19873734632 DE3734632A1 (de) 1987-10-13 1987-10-13 Verfahren zur herstellung von t-pa
DE19873738649 DE3738649A1 (de) 1987-11-13 1987-11-13 Verfahren zur herstellung von proteinen
DE?P3738649.2? 1987-11-13
DE19883801562 DE3801562A1 (de) 1988-01-20 1988-01-20 Verfahren zur herstellung von proteinen
DE?P3801562.5? 1988-01-20

Publications (1)

Publication Number Publication Date
KR890006671A true KR890006671A (ko) 1989-06-15

Family

ID=27196629

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019880013334A KR890006671A (ko) 1987-10-13 1988-10-13 단백질 제조방법

Country Status (16)

Country Link
EP (1) EP0315782B1 (ko)
JP (1) JP2688222B2 (ko)
KR (1) KR890006671A (ko)
AT (1) ATE112308T1 (ko)
AU (1) AU614999B2 (ko)
CA (1) CA1341400C (ko)
DE (1) DE3851685D1 (ko)
DK (1) DK175411B1 (ko)
ES (1) ES2064336T3 (ko)
FI (1) FI94963C (ko)
HU (1) HU205381B (ko)
IE (1) IE65986B1 (ko)
IL (1) IL88001A (ko)
NO (1) NO174778C (ko)
NZ (1) NZ226521A (ko)
PT (1) PT88751B (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2164050T3 (es) * 1991-04-18 2002-02-16 Toray Industries Preparacion de compuestos de interleuquina-6.
DE4113750A1 (de) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
CN1257549A (zh) * 1997-04-03 2000-06-21 吉富制药株式会社 异源蛋白的生产方法
EP1452597A4 (en) * 2001-12-04 2006-09-20 Mitsubishi Pharma Corp PROTEIN ACTIVATION METHOD
CA2511228A1 (en) * 2002-12-20 2004-07-08 Mitsubishi Pharma Corporation Method of protecting thiol group of protein
DK2154244T3 (en) * 2007-04-26 2017-06-12 Chugai Pharmaceutical Co Ltd CELL CULTIVATION PROCEDURE WHEN AN ACID-ENRICHED MEDIUM IS USED

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0163751B1 (en) * 1984-06-05 1989-09-20 Asahi Kasei Kogyo Kabushiki Kaisha Process for the preparation of a plasminogen activator
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein

Also Published As

Publication number Publication date
NO884549D0 (no) 1988-10-12
HU205381B (en) 1992-04-28
NZ226521A (en) 1991-04-26
HUT48306A (en) 1989-05-29
IL88001A (en) 1996-01-19
PT88751B (pt) 1995-03-01
PT88751A (pt) 1989-07-31
FI94963C (fi) 1995-11-27
JP2688222B2 (ja) 1997-12-08
ES2064336T3 (es) 1995-02-01
NO174778C (no) 1994-07-06
ATE112308T1 (de) 1994-10-15
JPH01231887A (ja) 1989-09-18
NO884549L (no) 1989-04-14
DK567888A (da) 1989-04-14
IE883079L (en) 1989-04-13
DE3851685D1 (de) 1994-11-03
CA1341400C (en) 2002-11-19
FI884652A0 (fi) 1988-10-11
EP0315782B1 (de) 1994-09-28
AU2373388A (en) 1989-05-11
FI94963B (fi) 1995-08-15
IE65986B1 (en) 1995-11-29
AU614999B2 (en) 1991-09-19
NO174778B (no) 1994-03-28
FI884652A (fi) 1989-04-14
IL88001A0 (en) 1989-06-30
DK567888D0 (da) 1988-10-12
DK175411B1 (da) 2004-09-27
EP0315782A2 (de) 1989-05-17
EP0315782A3 (en) 1989-09-20

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