ATE96167T1 - Verfahren zur kristallisation von enzymen. - Google Patents

Verfahren zur kristallisation von enzymen.

Info

Publication number
ATE96167T1
ATE96167T1 AT91902671T AT91902671T ATE96167T1 AT E96167 T1 ATE96167 T1 AT E96167T1 AT 91902671 T AT91902671 T AT 91902671T AT 91902671 T AT91902671 T AT 91902671T AT E96167 T1 ATE96167 T1 AT E96167T1
Authority
AT
Austria
Prior art keywords
enzymes
crystallization
concentration
enzyme
starting material
Prior art date
Application number
AT91902671T
Other languages
English (en)
Inventor
Niels-Viktor Nielsen
Torben Nielsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK654089A external-priority patent/DK654089D0/da
Priority claimed from DK84890A external-priority patent/DK84890D0/da
Priority claimed from DK84790A external-priority patent/DK84790D0/da
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE96167T1 publication Critical patent/ATE96167T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/98—Preparation of granular or free-flowing enzyme compositions

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Saccharide Compounds (AREA)
AT91902671T 1989-12-21 1990-12-21 Verfahren zur kristallisation von enzymen. ATE96167T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK654089A DK654089D0 (da) 1989-12-21 1989-12-21 Fremgangsmaade til krystallisation af enzymer og krystallinske enzymer
DK84890A DK84890D0 (da) 1990-04-05 1990-04-05 Fremgangsmaade til krystallisation af savinase
DK84790A DK84790D0 (da) 1990-04-05 1990-04-05 Fremgangsmaade til krystallisation af enzymer
PCT/DK1990/000341 WO1991009943A1 (en) 1989-12-21 1990-12-21 Method for crystallization of enzymes

Publications (1)

Publication Number Publication Date
ATE96167T1 true ATE96167T1 (de) 1993-11-15

Family

ID=27220958

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91902671T ATE96167T1 (de) 1989-12-21 1990-12-21 Verfahren zur kristallisation von enzymen.

Country Status (7)

Country Link
EP (1) EP0506866B1 (de)
JP (1) JP2975109B2 (de)
AT (1) ATE96167T1 (de)
DE (1) DE69004101T2 (de)
DK (1) DK0506866T3 (de)
ES (1) ES2060360T3 (de)
WO (1) WO1991009943A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5256557A (en) * 1991-12-27 1993-10-26 Solvay Enzymes, Inc. Purified alkaline protease concentrate and method of preparation
ATE195553T1 (de) 1992-06-09 2000-09-15 Chiron Corp Kristallisierung von m-csf
WO1994004665A1 (de) * 1992-08-14 1994-03-03 Solvay Enzymes Gmbh & Co. Kg Neue enzymgranulate
US5281526A (en) * 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
GB2287713A (en) * 1994-03-19 1995-09-27 Procter & Gamble Detergent composition containing pectic enzyme
US5789362A (en) * 1994-03-29 1998-08-04 The Procter & Gamble Co. Detergent composition comprising lipoxidase enzymes
DE69632669T2 (de) * 1995-07-28 2004-10-07 Basf Ag Salz-stabilisierte Enzymzubereitung
US6190898B1 (en) * 1995-10-23 2001-02-20 Genencor International, Inc. Crystalline cellulase and method for producing same
US6207437B1 (en) 1996-03-11 2001-03-27 Genencor International, Inc. Crystalline protease and method for producing same
TW409035B (en) 1997-06-04 2000-10-21 Gist Brocades Bv Starch-based enzyme granulates
ATE325868T1 (de) * 1999-03-05 2006-06-15 Genencor Int Verfahren zur schnellen herstellung von crystallen mit wünschenswerter morphologie
WO2002072636A2 (en) 2000-12-28 2002-09-19 Altus Biologics Inc. Crystals of whole antibodies and fragments thereof and methods for making and using them
WO2003000014A2 (en) 2001-06-21 2003-01-03 Altus Biologics, Inc. Spherical protein particles and methods of making and using them
WO2006012500A2 (en) * 2004-07-23 2006-02-02 Genentech, Inc. Crystallization of antibodies or fragments thereof
DK2125865T3 (da) 2007-03-15 2014-10-06 Novozymes As Opløsning af proteasekrystaller i fermenteringssuppe
US10023852B2 (en) 2014-05-27 2018-07-17 Novozymes A/S Lipase variants and polynucleotides encoding same
EP3878957A1 (de) 2014-05-27 2021-09-15 Novozymes A/S Verfahren zur herstellung von lipasen
EP4067485A3 (de) 2014-12-05 2023-01-04 Novozymes A/S Lipasevarianten und polynukleotide zur codierung davon
MX394221B (es) 2015-07-01 2025-03-24 Novozymes As Metodos de reduccion de olor.
CN107969136B (zh) 2015-07-06 2021-12-21 诺维信公司 脂肪酶变体以及编码它们的多核苷酸
WO2017093318A1 (en) 2015-12-01 2017-06-08 Novozymes A/S Methods for producing lipases
CN109790525A (zh) 2016-07-18 2019-05-21 诺维信公司 脂肪酶变体、编码其的多核苷酸及其用途
US11078445B2 (en) 2017-05-05 2021-08-03 Novozymes A/S Compositions comprising lipase and sulfite
US20200181542A1 (en) * 2017-06-30 2020-06-11 Novozymes A/S Enzyme Slurry Composition
CN111670248A (zh) 2017-12-04 2020-09-15 诺维信公司 脂肪酶变体以及编码其的多核苷酸

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL33327A (en) * 1968-11-29 1972-12-29 Lilly Co Eli A crystalline combination of l-asparaginase and a metal or ammonium or hydrazinium ion and method for preparing the same
DK8502857A (de) * 1984-06-25 1985-12-26
JP2886921B2 (ja) * 1988-03-18 1999-04-26 ジェネンカー インコーポレイテッド スブチリシンの結晶化法

Also Published As

Publication number Publication date
JPH05502791A (ja) 1993-05-20
EP0506866A1 (de) 1992-10-07
EP0506866B1 (de) 1993-10-20
JP2975109B2 (ja) 1999-11-10
ES2060360T3 (es) 1994-11-16
DK0506866T3 (da) 1994-03-21
DE69004101D1 (de) 1993-11-25
WO1991009943A1 (en) 1991-07-11
DE69004101T2 (de) 1994-03-24

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Legal Events

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UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee