ATE96167T1 - Verfahren zur kristallisation von enzymen. - Google Patents

Verfahren zur kristallisation von enzymen.

Info

Publication number
ATE96167T1
ATE96167T1 AT91902671T AT91902671T ATE96167T1 AT E96167 T1 ATE96167 T1 AT E96167T1 AT 91902671 T AT91902671 T AT 91902671T AT 91902671 T AT91902671 T AT 91902671T AT E96167 T1 ATE96167 T1 AT E96167T1
Authority
AT
Austria
Prior art keywords
enzymes
crystallization
concentration
enzyme
starting material
Prior art date
Application number
AT91902671T
Other languages
English (en)
Inventor
Niels-Viktor Nielsen
Torben Nielsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK654089A external-priority patent/DK654089D0/da
Priority claimed from DK84790A external-priority patent/DK84790D0/da
Priority claimed from DK84890A external-priority patent/DK84890D0/da
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE96167T1 publication Critical patent/ATE96167T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/98—Preparation of granular or free-flowing enzyme compositions

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Saccharide Compounds (AREA)
AT91902671T 1989-12-21 1990-12-21 Verfahren zur kristallisation von enzymen. ATE96167T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK654089A DK654089D0 (da) 1989-12-21 1989-12-21 Fremgangsmaade til krystallisation af enzymer og krystallinske enzymer
DK84790A DK84790D0 (da) 1990-04-05 1990-04-05 Fremgangsmaade til krystallisation af enzymer
DK84890A DK84890D0 (da) 1990-04-05 1990-04-05 Fremgangsmaade til krystallisation af savinase
PCT/DK1990/000341 WO1991009943A1 (en) 1989-12-21 1990-12-21 Method for crystallization of enzymes

Publications (1)

Publication Number Publication Date
ATE96167T1 true ATE96167T1 (de) 1993-11-15

Family

ID=27220958

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91902671T ATE96167T1 (de) 1989-12-21 1990-12-21 Verfahren zur kristallisation von enzymen.

Country Status (7)

Country Link
EP (1) EP0506866B1 (de)
JP (1) JP2975109B2 (de)
AT (1) ATE96167T1 (de)
DE (1) DE69004101T2 (de)
DK (1) DK0506866T3 (de)
ES (1) ES2060360T3 (de)
WO (1) WO1991009943A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5256557A (en) * 1991-12-27 1993-10-26 Solvay Enzymes, Inc. Purified alkaline protease concentrate and method of preparation
DE69329247T2 (de) * 1992-06-09 2001-05-31 Chiron Corp. (N.D.Ges.D. Staates Delaware), Emeryville Kristallisierung von m-csf
CA2142443A1 (en) * 1992-08-14 1994-03-03 Torsten W. Kiesser Novel enzyme granulates
US5281526A (en) * 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
GB2287713A (en) * 1994-03-19 1995-09-27 Procter & Gamble Detergent composition containing pectic enzyme
US5789362A (en) * 1994-03-29 1998-08-04 The Procter & Gamble Co. Detergent composition comprising lipoxidase enzymes
DE69632669T2 (de) * 1995-07-28 2004-10-07 Basf Ag Salz-stabilisierte Enzymzubereitung
US6190898B1 (en) * 1995-10-23 2001-02-20 Genencor International, Inc. Crystalline cellulase and method for producing same
US6207437B1 (en) * 1996-03-11 2001-03-27 Genencor International, Inc. Crystalline protease and method for producing same
TW409035B (en) 1997-06-04 2000-10-21 Gist Brocades Bv Starch-based enzyme granulates
MXPA01008715A (es) * 1999-03-05 2002-07-30 Genencor Int Metodo para obtener rapidamente cristales con morfologias deseables.
KR100923514B1 (ko) 2000-12-28 2009-10-27 알투스 파마슈티컬스 인코포레이티드 전항체 및 이의 단편의 결정과 이의 제조 및 사용 방법
CA2451185A1 (en) 2001-06-21 2003-01-03 Altus Biologics, Inc. Spherical protein particles and methods of making and using them
KR101320707B1 (ko) 2004-07-23 2013-10-22 제넨테크, 인크. 항체 또는 그의 단편의 결정화 방법
JP5453116B2 (ja) 2007-03-15 2014-03-26 ノボザイムス アクティーゼルスカブ 発酵ブロス中のプロテアーゼ結晶の可溶化
CN106459939A (zh) 2014-05-27 2017-02-22 诺维信公司 脂肪酶变体以及编码它们的多核苷酸
AR100605A1 (es) 2014-05-27 2016-10-19 Novozymes As Métodos para la producción de lipasas
EP3227442B1 (de) 2014-12-05 2022-02-16 Novozymes A/S Lipasevarianten und polynukleotide zur codierung davon
CA3175255A1 (en) 2015-07-01 2017-01-05 Novozymes A/S Methods of reducing odor
CN114292829A (zh) 2015-07-06 2022-04-08 诺维信公司 脂肪酶变体以及编码它们的多核苷酸
WO2017093318A1 (en) 2015-12-01 2017-06-08 Novozymes A/S Methods for producing lipases
EP4357453A3 (de) 2016-07-18 2025-01-22 Novozymes A/S Lipasevarianten, polynukleotide zur codierung davon und verwendung davon
EP3619304A1 (de) 2017-05-05 2020-03-11 Novozymes A/S Lipase und sulfit enthaltende zusammensetzungen
CN111108183A (zh) 2017-06-30 2020-05-05 诺维信公司 酶浆液组合物
CN111670248A (zh) 2017-12-04 2020-09-15 诺维信公司 脂肪酶变体以及编码其的多核苷酸

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL33327A (en) * 1968-11-29 1972-12-29 Lilly Co Eli A crystalline combination of l-asparaginase and a metal or ammonium or hydrazinium ion and method for preparing the same
DK8502857A (de) * 1984-06-25 1985-12-26
ATE114714T1 (de) * 1988-03-18 1994-12-15 Genencor Int Verfahren zur kristallisation von subtilisin.

Also Published As

Publication number Publication date
WO1991009943A1 (en) 1991-07-11
ES2060360T3 (es) 1994-11-16
JP2975109B2 (ja) 1999-11-10
DK0506866T3 (da) 1994-03-21
EP0506866A1 (de) 1992-10-07
EP0506866B1 (de) 1993-10-20
JPH05502791A (ja) 1993-05-20
DE69004101D1 (de) 1993-11-25
DE69004101T2 (de) 1994-03-24

Similar Documents

Publication Publication Date Title
ATE96167T1 (de) Verfahren zur kristallisation von enzymen.
DE59008910D1 (de) Stabile injizierbare lösungen von faktor viii.
GB2138004B (en) A process for the production of a protein
KR890003799A (ko) 염기성 단백질이 함유된 혼합물로부터 염기성 단백질을 분리하는 방법
AU3369299A (en) Stable concentrated rare earth carboxylate liquids
DE58905821D1 (de) Verfahren zur Herstellung von Des-B30-Insulinen und Des-B30-Insulinderivaten.
MX172150B (es) Composicion de detergente liquida que contiene enzima y sistema de estabilizacion
ATE87664T1 (de) Verfahren zur herstellung von optisch aktiven 2- arylpropionsaeuren.
AU576204B2 (en) Aqueous, injectable solutions of cis-platimum
RU94030485A (ru) Способ использования, хранения и транспортировки фермента металлопротеазы в стабилизированной форме и водная композиция фермента металлопротеазы
DE69011880D1 (de) Verfahren zur Herstellung von L-Aminosäuren durch Fermentation.
EP1329461B8 (de) Verfahren zur verhinderung von polymeren des humanen serumalbumins
DE68916754D1 (de) Verfahren zur Herstellung von optisch aktiver 3-Hydroxybuttersäure.
DE3463509D1 (en) Process for the dissolution of peptides and proteins, solutions thereby obtained and their use
GB1472429A (en) Nitrohumic acid-based adsorbent
ES8306994A1 (es) Procedimiento para la obtencion de diamidas de acido hidroxi-maloico sustituidas.
NL7017514A (en) Purification of proteins
GB1475263A (en) Mucopolysaccharides and their production
ATE114714T1 (de) Verfahren zur kristallisation von subtilisin.
CA2074182A1 (en) Process for the reactivation of denatured protein
DE3851685D1 (de) Verfahren zur Herstellung von Proteinen.
DE10299053I1 (de) Verfahren zur Verhinderung der Zersetzung des Proteins C
Yokoyama et al. Stabilization of crystalline acid carboxypeptidase from Penicillium janthinellum by nonionic surfactants, and inhibition of enzyme activity by anionic compounds
Castañeda-Agulló et al. On Dielectric Constant and Enzymatic Kinetics: III. Interrelationships of dielectric constant and pH
KR900007865A (ko) 융합 단백질의 선택적 절단 방법

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee