ATE114714T1 - Verfahren zur kristallisation von subtilisin. - Google Patents

Verfahren zur kristallisation von subtilisin.

Info

Publication number
ATE114714T1
ATE114714T1 AT89903871T AT89903871T ATE114714T1 AT E114714 T1 ATE114714 T1 AT E114714T1 AT 89903871 T AT89903871 T AT 89903871T AT 89903871 T AT89903871 T AT 89903871T AT E114714 T1 ATE114714 T1 AT E114714T1
Authority
AT
Austria
Prior art keywords
subtilisin
crystallization
solution
concentrate
concentrated
Prior art date
Application number
AT89903871T
Other languages
English (en)
Inventor
Todd Becker
Bryan Lawlis Virgil Jr
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE114714T1 publication Critical patent/ATE114714T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AT89903871T 1988-03-18 1989-03-17 Verfahren zur kristallisation von subtilisin. ATE114714T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US16999088A 1988-03-18 1988-03-18

Publications (1)

Publication Number Publication Date
ATE114714T1 true ATE114714T1 (de) 1994-12-15

Family

ID=22618060

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89903871T ATE114714T1 (de) 1988-03-18 1989-03-17 Verfahren zur kristallisation von subtilisin.

Country Status (5)

Country Link
EP (1) EP0404817B1 (de)
JP (1) JP2886921B2 (de)
AT (1) ATE114714T1 (de)
DE (1) DE68919688T2 (de)
WO (1) WO1989008703A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE96167T1 (de) * 1989-12-21 1993-11-15 Novo Nordisk As Verfahren zur kristallisation von enzymen.
US5837513A (en) * 1989-12-21 1998-11-17 Nielsen; Niels-Viktor Method for crystallization of enzymes
WO1991009928A1 (en) * 1989-12-21 1991-07-11 Novo Nordisk A/S Method for production of a clear, liquid detergent
WO1991009941A1 (en) * 1989-12-21 1991-07-11 Novo Nordisk A/S Enzyme containing preparation and detergent containing such preparation
US5281526A (en) * 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
CA2213122A1 (en) * 1994-12-28 1996-07-04 Genencor International, Inc. Enzyme stabilization
US6207437B1 (en) 1996-03-11 2001-03-27 Genencor International, Inc. Crystalline protease and method for producing same
ATE325868T1 (de) * 1999-03-05 2006-06-15 Genencor Int Verfahren zur schnellen herstellung von crystallen mit wünschenswerter morphologie

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5739782A (en) * 1980-08-22 1982-03-05 Toyo Soda Mfg Co Ltd Preparation of saturated enzyme solution
JPH08239782A (ja) * 1995-02-28 1996-09-17 Hitachi Chem Co Ltd ウエット処理装置

Also Published As

Publication number Publication date
DE68919688D1 (de) 1995-01-12
WO1989008703A1 (en) 1989-09-21
EP0404817B1 (de) 1994-11-30
EP0404817A1 (de) 1991-01-02
JPH03504320A (ja) 1991-09-26
JP2886921B2 (ja) 1999-04-26
DE68919688T2 (de) 1995-04-13

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee