ES2060360T3 - Metodo para la cristalizacion de enzimas. - Google Patents

Metodo para la cristalizacion de enzimas.

Info

Publication number
ES2060360T3
ES2060360T3 ES91902671T ES91902671T ES2060360T3 ES 2060360 T3 ES2060360 T3 ES 2060360T3 ES 91902671 T ES91902671 T ES 91902671T ES 91902671 T ES91902671 T ES 91902671T ES 2060360 T3 ES2060360 T3 ES 2060360T3
Authority
ES
Spain
Prior art keywords
enzymes
crystallization
concentration
enzyme
starting material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91902671T
Other languages
English (en)
Inventor
Niels-Viktor Nielsen
Torben Kjaersgaard Nielsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK654089A external-priority patent/DK654089D0/da
Priority claimed from DK84890A external-priority patent/DK84890D0/da
Priority claimed from DK84790A external-priority patent/DK84790D0/da
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2060360T3 publication Critical patent/ES2060360T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/98—Preparation of granular or free-flowing enzyme compositions

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

METODO DE CRISTALIZACION DE ENZIMAS. EL METODO DE CRISTALIZACION DE ENZIMAS ESTA CARACTERIZADO POR EL HECHO DE QUE UNA ENZIMA ACUOSA QUE CONTIENE LIQUIDO CON UNA PUREZA ENZIMATICA RELATIVAMENTE ALTA Y CON UNA CONCENTRACION DE PROTEINA ENZIMATICA PURA DE AL MENOS 5 G/L DE LA ENZIMA QUE CONTIENE LIQUIDO SE UTILIZA COMO UNA MATERIA PRIMA, Y EN QUE UN AGENTE DE CRISTALIZACION, EL CUAL ES UNA SAL FACILMENTE SOLUBLE DE TIPO NO HALURO SE AÑADE A LA MATERIA PRIMA PARA UNA CONCENTRACION FINAL QUE ES CONSIDERABLEMENTE INFERIOR A LA CONCENTRACION NECESARIA PARA PRECIPITAR LAS ENZIMAS EN UNA FORMA AMORFA. EL METODO ES SENCILLO Y BARATO, Y ES COMPATIBLE CON NECESIDADES INDUSTRIALES.
ES91902671T 1989-12-21 1990-12-21 Metodo para la cristalizacion de enzimas. Expired - Lifetime ES2060360T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK654089A DK654089D0 (da) 1989-12-21 1989-12-21 Fremgangsmaade til krystallisation af enzymer og krystallinske enzymer
DK84890A DK84890D0 (da) 1990-04-05 1990-04-05 Fremgangsmaade til krystallisation af savinase
DK84790A DK84790D0 (da) 1990-04-05 1990-04-05 Fremgangsmaade til krystallisation af enzymer

Publications (1)

Publication Number Publication Date
ES2060360T3 true ES2060360T3 (es) 1994-11-16

Family

ID=27220958

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91902671T Expired - Lifetime ES2060360T3 (es) 1989-12-21 1990-12-21 Metodo para la cristalizacion de enzimas.

Country Status (7)

Country Link
EP (1) EP0506866B1 (es)
JP (1) JP2975109B2 (es)
AT (1) ATE96167T1 (es)
DE (1) DE69004101T2 (es)
DK (1) DK0506866T3 (es)
ES (1) ES2060360T3 (es)
WO (1) WO1991009943A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5256557A (en) * 1991-12-27 1993-10-26 Solvay Enzymes, Inc. Purified alkaline protease concentrate and method of preparation
EP0955365A3 (en) 1992-06-09 2000-12-20 Chiron Corporation Crystallization of M-CSF
JPH08500375A (ja) * 1992-08-14 1996-01-16 ゾルファイ エンツィーメス ゲゼルシャフト ミツト ベシユレンクテル ハフツング ウント コンパニー コマンディートゲゼルシャフト 新規の酵素粒状物
US5281526A (en) * 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
GB2287713A (en) * 1994-03-19 1995-09-27 Procter & Gamble Detergent composition containing pectic enzyme
US5789362A (en) * 1994-03-29 1998-08-04 The Procter & Gamble Co. Detergent composition comprising lipoxidase enzymes
PL319347A1 (en) * 1995-07-28 1997-08-04 Gist Brocades Bv Method of obtaining salt-stabilised enzymes
US6190898B1 (en) * 1995-10-23 2001-02-20 Genencor International, Inc. Crystalline cellulase and method for producing same
US6207437B1 (en) 1996-03-11 2001-03-27 Genencor International, Inc. Crystalline protease and method for producing same
TW409035B (en) 1997-06-04 2000-10-21 Gist Brocades Bv Starch-based enzyme granulates
ATE325868T1 (de) * 1999-03-05 2006-06-15 Genencor Int Verfahren zur schnellen herstellung von crystallen mit wünschenswerter morphologie
IL156618A0 (en) 2000-12-28 2004-01-04 Altus Biologics Inc Crystals of whole antibodies and fragments thereof, methods for the preparation thereof and diagnostic kits utilizing the same
WO2003000014A2 (en) 2001-06-21 2003-01-03 Altus Biologics, Inc. Spherical protein particles and methods of making and using them
ZA200700947B (en) * 2004-07-23 2008-06-25 Genentech Inc Crystallization of antibodies or fragments thereof
DK2125865T3 (da) 2007-03-15 2014-10-06 Novozymes As Opløsning af proteasekrystaller i fermenteringssuppe
EP3878957A1 (en) 2014-05-27 2021-09-15 Novozymes A/S Methods for producing lipases
AR100606A1 (es) 2014-05-27 2016-10-19 Novozymes As Variantes de lipasas y polinucleótidos que las codifican
EP4067485A3 (en) 2014-12-05 2023-01-04 Novozymes A/S Lipase variants and polynucleotides encoding same
CA3175255A1 (en) 2015-07-01 2017-01-05 Novozymes A/S Methods of reducing odor
EP3320089B1 (en) 2015-07-06 2021-06-16 Novozymes A/S Lipase variants and polynucleotides encoding same
CN108431217B (zh) 2015-12-01 2022-06-21 诺维信公司 用于产生脂肪酶的方法
US11326152B2 (en) 2016-07-18 2022-05-10 Novozymes A/S Lipase variants, polynucleotides encoding same and the use thereof
WO2018202846A1 (en) 2017-05-05 2018-11-08 Novozymes A/S Compositions comprising lipase and sulfite
WO2019002356A1 (en) 2017-06-30 2019-01-03 Novozymes A/S ENZYMATIC SUSPENSION COMPOSITION
WO2019110462A1 (en) 2017-12-04 2019-06-13 Novozymes A/S Lipase variants and polynucleotides encoding same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL33327A (en) * 1968-11-29 1972-12-29 Lilly Co Eli A crystalline combination of l-asparaginase and a metal or ammonium or hydrazinium ion and method for preparing the same
DK8502857A (es) * 1984-06-25 1985-12-26
ATE114714T1 (de) * 1988-03-18 1994-12-15 Genencor Int Verfahren zur kristallisation von subtilisin.

Also Published As

Publication number Publication date
EP0506866B1 (en) 1993-10-20
EP0506866A1 (en) 1992-10-07
DE69004101T2 (de) 1994-03-24
DE69004101D1 (de) 1993-11-25
WO1991009943A1 (en) 1991-07-11
DK0506866T3 (da) 1994-03-21
ATE96167T1 (de) 1993-11-15
JPH05502791A (ja) 1993-05-20
JP2975109B2 (ja) 1999-11-10

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