ES8800957A1 - Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina - Google Patents

Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina

Info

Publication number
ES8800957A1
ES8800957A1 ES552233A ES552233A ES8800957A1 ES 8800957 A1 ES8800957 A1 ES 8800957A1 ES 552233 A ES552233 A ES 552233A ES 552233 A ES552233 A ES 552233A ES 8800957 A1 ES8800957 A1 ES 8800957A1
Authority
ES
Spain
Prior art keywords
naturation
somatotropin
solution
protein
solubilization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES552233A
Other languages
English (en)
Other versions
ES552233A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27107301&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES8800957(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/704,677 external-priority patent/US4652630A/en
Application filed by Monsanto Co filed Critical Monsanto Co
Priority to US06/900,018 priority Critical patent/US4731440A/en
Publication of ES552233A0 publication Critical patent/ES552233A0/es
Publication of ES8800957A1 publication Critical patent/ES8800957A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormones [GH] (Somatotropin)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Cosmetics (AREA)
  • Dental Preparations (AREA)

Abstract

METODO PARA LA SOLUBILIZACION Y RENATURALIZACION DE PROTEINA SOMATOTROPINA DE CUERPOS REFRACTIVOS DE UNA CELULA HUESPED. COMPRENDE: A) PONER EN CONTACTO LOS CUERPOS CON UNA CANTIDAD Y CONCENTRACION EFECTIVAS DE UNA SOLUCION ACUOSA DE UREA O DIMETILSULFONA, A UN PH DE 9 A 12 Y A UNA TEMPERATURA ENTRE 25JC Y EL PUNTO DE CONGELACION DE LA SOLUCION, PARA LOGRAR LA SOLUBILIZACION DE LA CITADA PROTEIPNA; Y B) PONER EN CONTACTO LA SOLUCION CONTENIENDO LA PROTEINA CON UN AGENTE OXIDANTE SUAVE, DURANTE UN PERIODO DE TIEMPO SUFICIENTE PARA FORMAR ENLACES DISULFURO INTRAMOLECULARES ENTRE LOS RESIDUOS DE CISTEINA CONTENIDOS EN LA PROTEINA.
ES552233A 1985-02-22 1986-02-20 Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina Expired ES8800957A1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US06/900,018 US4731440A (en) 1985-02-22 1986-08-25 Method of somatotropin solubilization using dimethylsulfone and naturation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70434185A 1985-02-22 1985-02-22
US06/704,677 US4652630A (en) 1985-02-22 1985-02-22 Method of somatotropin naturation

Publications (2)

Publication Number Publication Date
ES552233A0 ES552233A0 (es) 1987-12-01
ES8800957A1 true ES8800957A1 (es) 1987-12-01

Family

ID=27107301

Family Applications (1)

Application Number Title Priority Date Filing Date
ES552233A Expired ES8800957A1 (es) 1985-02-22 1986-02-20 Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina

Country Status (14)

Country Link
EP (1) EP0192629B2 (es)
JP (1) JP2572963B2 (es)
KR (1) KR890003082B1 (es)
CN (1) CN1010097B (es)
AT (1) ATE66930T1 (es)
AU (3) AU587423B2 (es)
CA (1) CA1268300A (es)
DE (1) DE3681185D1 (es)
DK (1) DK172526B1 (es)
ES (1) ES8800957A1 (es)
IE (1) IE58871B1 (es)
IL (1) IL77951A (es)
NZ (1) NZ215259A (es)
SU (1) SU1542402A3 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU612133B2 (en) * 1987-02-20 1991-07-04 Natinco Nv Production of proteins in active forms
US4985544A (en) * 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
JP2669859B2 (ja) * 1987-08-04 1997-10-29 協和醗酵工業株式会社 タンパク質の再活性化法
JPH01132598A (ja) * 1987-10-14 1989-05-25 Pitman Moore Inc 変性剤溶液中に含まれる組換え蛋白質における分子内ジスルフィド結合の生成を促進する方法
JPH01257491A (ja) * 1988-04-08 1989-10-13 Tosoh Corp 不溶性融合異種蛋白質の処理方法
US5089473A (en) 1988-08-29 1992-02-18 Monsanto Company Somatotropin variants and their use
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5064943A (en) * 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
JP2517100B2 (ja) * 1989-03-08 1996-07-24 協和醗酵工業株式会社 ヒト顆粒球コロニ―刺激因子活性を有する蛋白質の精製法
US5182369A (en) * 1990-02-28 1993-01-26 Monsanto Company Method for purifying somatotropin monomers
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
AU2592395A (en) * 1994-05-19 1995-12-18 Monsanto Company Method for purifying somatotropin monomers
JPH11505507A (ja) * 1994-07-25 1999-05-21 ノバルティス アクチェンゲゼルシャフト 組換えヒルジンまたは表皮増殖因子のようなタンパク質の折りたたみ方法
ZA9711733B (en) 1996-12-31 1998-07-01 Monsanto Co Method for solubilization and naturation of somatotropins
EP0984789A2 (en) * 1997-06-27 2000-03-15 Monsanto Company Accelerating animal hoof growth with somatotropin
JP2009504571A (ja) * 2005-07-25 2009-02-05 トルービオン ファーマスーティカルズ インコーポレイテッド タンパク質解離のための組成物および方法
JP4766052B2 (ja) 2005-11-24 2011-09-07 株式会社村田製作所 電気音響変換器
US20080032343A1 (en) 2005-12-22 2008-02-07 Genentech, Inc. Recombinant Production of Heparin Binding Proteins
AU2007272412B2 (en) * 2006-07-14 2013-11-07 Genentech, Inc. Refolding of recombinant proteins
CN110249048A (zh) * 2016-09-15 2019-09-17 太阳基因组学公司 用于从样品中的一种或多种类型的微生物的多样性群体中提取核酸分子的通用方法
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE55163B1 (en) * 1982-06-07 1990-06-20 Celltech Ltd A process for the preparation of chymosin
GR79124B (es) * 1982-12-22 1984-10-02 Genentech Inc
GB2138004B (en) * 1983-03-25 1987-05-13 Celltech Ltd A process for the production of a protein

Also Published As

Publication number Publication date
AU587423B2 (en) 1989-08-17
IL77951A (en) 1990-11-05
SU1542402A3 (ru) 1990-02-07
AU3498089A (en) 1989-09-14
ATE66930T1 (de) 1991-09-15
ES552233A0 (es) 1987-12-01
AU5385386A (en) 1986-08-28
DK172526B1 (da) 1998-11-16
AU618386B2 (en) 1991-12-19
EP0192629B2 (en) 1997-03-05
CA1268300C (en) 1990-04-24
NZ215259A (en) 1989-11-28
AU621111B2 (en) 1992-03-05
AU3925889A (en) 1990-04-05
DK80986A (da) 1986-08-23
JPS61195698A (ja) 1986-08-29
KR860006541A (ko) 1986-09-13
EP0192629A2 (en) 1986-08-27
IE860466L (en) 1986-08-22
CN86100915A (zh) 1986-08-20
KR890003082B1 (ko) 1989-08-21
EP0192629B1 (en) 1991-09-04
CA1268300A (en) 1990-04-24
DE3681185D1 (de) 1991-10-10
IE58871B1 (en) 1993-11-17
CN1010097B (zh) 1990-10-24
EP0192629A3 (en) 1988-07-27
DK80986D0 (da) 1986-02-21
JP2572963B2 (ja) 1997-01-16

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