ATE80166T1 - Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form. - Google Patents

Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form.

Info

Publication number
ATE80166T1
ATE80166T1 AT86906674T AT86906674T ATE80166T1 AT E80166 T1 ATE80166 T1 AT E80166T1 AT 86906674 T AT86906674 T AT 86906674T AT 86906674 T AT86906674 T AT 86906674T AT E80166 T1 ATE80166 T1 AT E80166T1
Authority
AT
Austria
Prior art keywords
polypeptide
disulfide bonds
reducing
disulfide
isolation
Prior art date
Application number
AT86906674T
Other languages
English (en)
Inventor
Pradip Ghosh-Dastidar
Original Assignee
Int Genetic Eng
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Int Genetic Eng filed Critical Int Genetic Eng
Application granted granted Critical
Publication of ATE80166T1 publication Critical patent/ATE80166T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
AT86906674T 1985-11-13 1986-10-28 Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form. ATE80166T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US06/797,473 US4766205A (en) 1985-11-13 1985-11-13 Method for isolation of recombinant polypeptides in biologically active forms
EP86906674A EP0246279B1 (de) 1985-11-13 1986-10-28 Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form
PCT/US1986/002264 WO1987002985A1 (en) 1985-11-13 1986-10-28 Method for isolation of recombinant polypeptides in biologically active forms

Publications (1)

Publication Number Publication Date
ATE80166T1 true ATE80166T1 (de) 1992-09-15

Family

ID=25170929

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86906674T ATE80166T1 (de) 1985-11-13 1986-10-28 Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form.

Country Status (8)

Country Link
US (1) US4766205A (de)
EP (1) EP0246279B1 (de)
JP (1) JPS63501291A (de)
AT (1) ATE80166T1 (de)
AU (1) AU582927B2 (de)
CA (1) CA1265461A (de)
DE (1) DE3686654T2 (de)
WO (1) WO1987002985A1 (de)

Families Citing this family (36)

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Publication number Priority date Publication date Assignee Title
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4985544A (en) * 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
JPH01132598A (ja) * 1987-10-14 1989-05-25 Pitman Moore Inc 変性剤溶液中に含まれる組換え蛋白質における分子内ジスルフィド結合の生成を促進する方法
US5079230A (en) * 1988-09-12 1992-01-07 Pitman-Moore, Inc. Stable bioactive somatotropins
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
IL96682A0 (en) * 1989-12-15 1991-09-16 Amgen Inc Production of biologically active platelet-derived growth factor from high expression host cell systems
US5986070A (en) * 1990-04-06 1999-11-16 Amgen Inc. Production of biologically active NGF proteins
US5235043A (en) * 1990-04-06 1993-08-10 Synergen, Inc. Production of biologically active, recombinant members of the ngf/bdnf family of neurotrophic proteins
WO1992003477A1 (en) * 1990-08-20 1992-03-05 Novo Nordisk A/S Biologically active compound, a process for the preparation thereof and use of the same
AU664021B2 (en) * 1990-09-05 1995-11-02 Natinco Nv Solubilization of proteins in active forms
US5235041A (en) * 1990-12-28 1993-08-10 Protein Polymer Technologies, Inc. Purification of structurally ordered recombinant protein polymers
US5326580A (en) * 1993-02-16 1994-07-05 Wisconsin Alumni Research Foundation Brazzein sweetener
CN1105029A (zh) * 1993-05-24 1995-07-12 花王株式会社 制备溶解蛋白质的方法
AU2603995A (en) * 1994-05-25 1995-12-18 University Of Nebraska Board Of Regents Biologically active glycoprotein hormones produced in procaryotic cells
US5563057A (en) * 1994-10-31 1996-10-08 Wisconsin Alumni Research Foundation Method for refolding misfolded enzymes with detergent and cyclodextrin
US5789547A (en) * 1995-06-07 1998-08-04 Celtrix Pharmaceuticals, Inc. Method of producing insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) with correct folding and disulfide bonding
BR9709569B1 (pt) * 1996-06-11 2010-05-18 processo para ativação de proteìna desnaturada.
ZA9711733B (en) 1996-12-31 1998-07-01 Monsanto Co Method for solubilization and naturation of somatotropins
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
SK10472003A3 (sk) * 2001-02-23 2004-03-02 Immunex Corporation Zvýšený výťažok aktívnych proteínov
WO2003046195A1 (en) * 2001-11-30 2003-06-05 Novozymes A/S Method for generating a site-specific library of variants
WO2004072292A1 (fr) * 2003-02-11 2004-08-26 Centre National En Electrochimie Et En Technologies Environnementales Inc. Procede et dispositif pour la production de biomolecules en continu
JP5553963B2 (ja) * 2004-10-22 2014-07-23 アムジエン・インコーポレーテツド 組換え抗体をリフォールディングする方法
MX2010002683A (es) * 2007-09-14 2010-03-26 Amgen Inc Poblaciones de anticuerpos homogeneos.
RS61000B1 (sr) 2014-09-01 2020-11-30 Int Centre For Genetic Engineering And Biotechnology Vakcina
AR103172A1 (es) 2014-12-22 2017-04-19 Novartis Ag Reducción selectiva de residuos de cisteina en anticuerpos il-17

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2129810B (en) * 1982-06-07 1985-09-18 Celltech Ltd A process for the preparation of chymosin
AU538170B2 (en) * 1982-09-15 1984-08-02 Owens-Illinois Glass Container Inc. Modified protein to mould an enzyme type molecule
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
GR79124B (de) * 1982-12-22 1984-10-02 Genentech Inc
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
JPH06102034B2 (ja) * 1983-03-25 1994-12-14 セルテク リミテツド タンパク質の生産方法
JPS6079000A (ja) * 1983-10-04 1985-05-04 Takeda Chem Ind Ltd ヒトγ型インタ−フエロン単量体の製造法
EP0155832A3 (de) * 1984-03-16 1987-08-19 Genentech, Inc. Stabile Proteine, Verfahren zu deren Herstellung, dieselben kodierende Plasmide und andere DNS, diese Plasmide enthaltende Zellkulturen
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten

Also Published As

Publication number Publication date
DE3686654D1 (de) 1992-10-08
AU582927B2 (en) 1989-04-13
WO1987002985A1 (en) 1987-05-21
US4766205A (en) 1988-08-23
EP0246279A4 (de) 1989-05-11
JPS63501291A (ja) 1988-05-19
DE3686654T2 (de) 1993-02-18
CA1265461A (en) 1990-02-06
AU6591686A (en) 1987-06-02
EP0246279B1 (de) 1992-09-02
EP0246279A1 (de) 1987-11-25

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee