LV10652B - Novel proteolytic enzymes and their use in detergents - Google Patents

Novel proteolytic enzymes and their use in detergents Download PDF

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Publication number
LV10652B
LV10652B LVP-93-1384A LV931384A LV10652B LV 10652 B LV10652 B LV 10652B LV 931384 A LV931384 A LV 931384A LV 10652 B LV10652 B LV 10652B
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LV
Latvia
Prior art keywords
protease
amino acid
enzyme
mutant
product according
Prior art date
Application number
LVP-93-1384A
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English (en)
Latvian (lv)
Other versions
LV10652A (lv
Inventor
Eekelen Christiaan A G Van
Leonardus J S M Mulleners
Van Der Laan Johannes Cornelis
Misset Onno
Cuperus Roelck Anneke
Lensink Johan Herman Albert
Original Assignee
Gist Brocades Nv
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8199752&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=LV10652(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of LV10652A publication Critical patent/LV10652A/xx
Publication of LV10652B publication Critical patent/LV10652B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
LVP-93-1384A 1988-02-11 1993-12-28 Novel proteolytic enzymes and their use in detergents LV10652B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP88200255 1988-02-11
PCT/NL1989/000005 WO1989007642A1 (en) 1988-02-11 1989-02-13 Novel proteolytic enzymes and their use in detergents

Publications (2)

Publication Number Publication Date
LV10652A LV10652A (lv) 1995-04-20
LV10652B true LV10652B (en) 1995-08-20

Family

ID=8199752

Family Applications (1)

Application Number Title Priority Date Filing Date
LVP-93-1384A LV10652B (en) 1988-02-11 1993-12-28 Novel proteolytic enzymes and their use in detergents

Country Status (21)

Country Link
US (1) US5336611A (lt)
EP (3) EP0328229B2 (lt)
JP (1) JP2849141B2 (lt)
KR (1) KR970005249B1 (lt)
CN (1) CN1056187C (lt)
AT (1) ATE250669T1 (lt)
AU (1) AU629814B2 (lt)
BG (1) BG60566B1 (lt)
BR (1) BR8905580A (lt)
CA (1) CA1340366C (lt)
DE (2) DE68912359T3 (lt)
DK (1) DK175813B1 (lt)
ES (1) ES2061929T5 (lt)
FI (1) FI105481B (lt)
GR (1) GR3021934T3 (lt)
IE (2) IE940164L (lt)
LT (1) LT3963B (lt)
LV (1) LV10652B (lt)
PT (1) PT89702B (lt)
RU (1) RU2069695C1 (lt)
WO (1) WO1989007642A1 (lt)

Families Citing this family (127)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5185258A (en) † 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
ATE139567T1 (de) * 1987-04-03 1996-07-15 Amgen Inc Proteolytische enzyme
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
BR9006827A (pt) * 1989-06-26 1991-08-06 Unilever Nv Composicoes detergentes enzimaticas
ATE144283T1 (de) * 1989-08-11 1996-11-15 Gist Brocades Nv Ergiebige herstellung von protease-mutanten
US5352603A (en) * 1989-08-31 1994-10-04 Kali-Chemie Ag Highly alkaline proteases
DE4023458A1 (de) * 1989-08-31 1991-03-07 Kali Chemie Ag Neue hochalkalische proteasen
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
DK97190D0 (da) * 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
DK0486073T3 (da) * 1990-11-14 1996-12-09 Procter & Gamble Væskeformig detergentsammensætning, der indeholder lipase og protease
US5733473A (en) * 1990-11-14 1998-03-31 The Procter & Gamble Company Liquid detergent composition containing lipase and protease
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
GB9027836D0 (en) * 1990-12-21 1991-02-13 Unilever Plc Enzymes and enzymatic detergent compositions
DE4106525A1 (de) * 1991-03-01 1992-09-10 Solvay Enzymes Gmbh & Co Kg Verfahren zur verbesserung der stabilitaet von hochalkalischen proteasen
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
DE69226182T2 (de) * 1991-05-01 1999-01-21 Novo Nordisk A/S, Bagsvaerd Stabilisierte enzyme und waschmittelzusammensetzungen
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5510048A (en) * 1991-05-31 1996-04-23 Colgate Palmolive Co. Nonaqueous liquid, phosphate-free, improved autoamatic dishwashing composition containing enzymes
EP0995801A1 (de) * 1991-07-27 2000-04-26 Genencor International GmbH Verfahren zur Verbesserung des Stabilität von Enzymen und stabilisierte Enzyme
US5792623A (en) * 1992-04-06 1998-08-11 Immuno Aktiengesellschaft Method for producing activated blood factors with a protease and a detergent
AT397390B (de) * 1992-04-06 1994-03-25 Immuno Ag Verfahren zur spaltung von proteinen
EP0571014B1 (en) * 1992-05-18 2004-03-31 Genencor International, Inc. Bacteria producing alkaline proteases, and production of these alkaline proteases
JP4028592B2 (ja) * 1992-07-17 2007-12-26 ジェネンコー インターナショナル インコーポレイテッド 高アルカリ性セリンプロテアーゼ
WO1994002597A1 (en) * 1992-07-23 1994-02-03 Novo Nordisk A/S MUTANT α-AMYLASE, DETERGENT, DISH WASHING AGENT, AND LIQUEFACTION AGENT
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
DE4304161A1 (de) * 1993-02-12 1994-08-25 Solvay Enzymes Gmbh & Co Kg Verbesserte hochalkalische Proteasen
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
ES2287931T3 (es) * 1993-10-14 2007-12-16 THE PROCTER & GAMBLE COMPANY Composiciones limpiadoras que contienen proteasa.
MA23346A1 (fr) 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
US5932532A (en) * 1993-10-14 1999-08-03 Procter & Gamble Company Bleach compositions comprising protease enzyme
AU1253695A (en) * 1993-10-14 1995-05-04 Procter & Gamble Company, The Bleaching compositions comprising protease enzymes
ES2364776T3 (es) * 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
EP0766727B1 (en) * 1994-06-17 2002-08-14 Genencor International, Inc. Cleaning method based on compositions containing a hemicellulase plant cell wall degrading enzyme and the use thereof in cleaning methods
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US5714373A (en) * 1995-02-27 1998-02-03 Recombinant Biocatalysis, Inc. Thermococcus AV4 and enzymes produced by the same
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
CA2273079C (en) * 1996-11-26 2012-04-10 Genencor International, Inc. Chemically modified subtilisin mutants
AR015977A1 (es) * 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
RU2269572C2 (ru) * 1997-10-23 2006-02-10 Джененкор Интернэшнл, Инк. Варианты протеазы, замещенные в нескольких положениях
ES2368718T3 (es) 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
JP2002500874A (ja) 1998-01-23 2002-01-15 ジェネンコア インターナショナル インコーポレーテッド ペプチド合成のための修飾された酵素およびその使用方法
US6642011B2 (en) 1998-04-15 2003-11-04 Genencor International, Inc. Human protease and use of such protease for pharmaceutical applications and for reducing the allergenicity of non-human proteins
EP1093459B1 (en) 1998-07-02 2008-09-10 The Governing Council Of The University Of Toronto Chemically modified proteins with a carbohydrate moiety
US6831053B1 (en) 1998-10-23 2004-12-14 The Procter & Gamble Company Bleaching compositions comprising multiply-substituted protease variants
DE69940978D1 (de) 1998-11-10 2009-07-23 Univ Toronto Chemisch modifizierte mutante serinhydrolasen
EP1141335B1 (en) 1998-12-21 2009-08-05 Genencor International, Inc. Chemically modified enzymes with multiple charged variants
KR20000065867A (ko) * 1999-04-09 2000-11-15 손경식 세탁세제용 알칼리성 단백질 분해효소 브이에이피케이, 이를 코딩하는 유전자, 재조합 발현벡터 및 형질전환된 균주
JP5180417B2 (ja) 1999-04-28 2013-04-10 ジェネンコー・インターナショナル・インク 特異的に標的化された触媒性アンタゴニストおよびその使用
US6627744B2 (en) 1999-07-02 2003-09-30 Genencor International, Inc. Synthesis of glycodendrimer reagents
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6541234B1 (en) * 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
JP2005535284A (ja) 2001-12-31 2005-11-24 ジェネンコー・インターナショナル・インク 免疫反応の変化を生じるプロテアーゼ、ならびにその製造および利用方法
DK1523553T3 (da) * 2002-01-16 2010-04-19 Genencor Int Multi-substituerede proteasevarianter
AU2003210551A1 (en) * 2002-01-16 2003-09-02 Genencor International, Inc. Multiply-substituted protease variants
US8124399B2 (en) 2002-03-29 2012-02-28 Danisco Us Inc. Enhanced protein expression in Bacillus
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
DE10260903A1 (de) * 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
EP1735339A2 (en) * 2004-04-09 2006-12-27 Genencor International, Inc. Pcka modifications and enhanced protein expression in bacillus
CA2643265C (en) * 2006-03-02 2014-07-29 Genencor Division Danisco Us, Inc. Surface active bleach and dynamic ph
KR20150115023A (ko) * 2006-07-18 2015-10-13 다니스코 유에스 인크. 광범위한 온도에 걸쳐 활성인 프로테아제 변이형
US20080090745A1 (en) * 2006-10-13 2008-04-17 Fox Bryan P Expression of Streptomyces subtilism inhibitor (SSI) proteins in Bacillus and Streptomyces sp.
JP5867984B2 (ja) * 2007-03-12 2016-02-24 ダニスコ・ユーエス・インク 修飾プロテアーゼ
US8343735B2 (en) * 2007-05-10 2013-01-01 Danisco Us Inc. Modified secretion system to increase expression of polypeptides in bacteria
RU2520808C2 (ru) * 2007-06-06 2014-06-27 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН Способ идентификации улучшенных вариантов белка
BRPI0812446A2 (pt) 2007-06-06 2014-10-14 Danisco Us Inc Genecor Division Métodos para aperfeiçoamento de propriedades de proteína múltiplas
DE102007044415A1 (de) 2007-09-17 2009-03-19 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
JP4775975B2 (ja) * 2007-12-14 2011-09-21 キャニヨン株式会社 蓄圧式ディスペンサー
AU2008348270A1 (en) 2007-12-21 2009-07-30 Danisco Us Inc. Enhanced protein production in bacillus
US20090233830A1 (en) * 2008-03-14 2009-09-17 Penny Sue Dirr Automatic detergent dishwashing composition
EP2100947A1 (en) 2008-03-14 2009-09-16 The Procter and Gamble Company Automatic dishwashing detergent composition
WO2009120929A1 (en) * 2008-03-28 2009-10-01 Danisco Us Inc., Genencor Division Method for amplifying locus in bacterial cell
CA2743123A1 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising a subtilisin variant
BRPI0920925A2 (pt) 2008-11-11 2015-08-18 Danisco Us Inc Composições e métodos compreendendo uma variante de subtilisina
ES2461892T3 (es) * 2009-02-02 2014-05-21 The Procter & Gamble Company Composición detergente líquida para lavado de vajillas a mano
MX2011008159A (es) * 2009-02-02 2011-11-18 Procter & Gamble Composicion detergente liquida para el lavado manual de vajillas.
EP2216391A1 (en) * 2009-02-02 2010-08-11 The Procter & Gamble Company Liquid hand dishwashing detergent composition
EP2216392B1 (en) * 2009-02-02 2013-11-13 The Procter and Gamble Company Liquid hand dishwashing detergent composition
EP3023483A1 (en) * 2009-02-02 2016-05-25 The Procter and Gamble Company Liquid hand diswashing detergent composition
EP2216390B1 (en) * 2009-02-02 2013-11-27 The Procter and Gamble Company Hand dishwashing method
EP2216393B1 (en) * 2009-02-09 2024-04-24 The Procter & Gamble Company Detergent composition
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
GB0915572D0 (en) * 2009-09-07 2009-10-07 Reckitt Benckiser Nv Detergent composition
CN104946427A (zh) * 2009-09-25 2015-09-30 诺维信公司 蛋白酶变体的用途
BR112012028467B1 (pt) * 2010-05-06 2021-02-17 Danisco Us Inc. variantes de subtilisina, seu método de produção, ácido nucleico, e método de limpeza
US9434932B2 (en) 2011-06-30 2016-09-06 Novozymes A/S Alpha-amylase variants
CN102277344B (zh) * 2011-08-03 2012-12-12 天津科技大学 一种低温碱性蛋白酶及其制备方法
DE102013219467A1 (de) * 2013-09-26 2015-03-26 Henkel Ag & Co. Kgaa Stabilisierte Inhibitor-Proteasevarianten
MX2016008595A (es) 2013-12-31 2016-10-28 Danisco Us Inc Expresion mejorada de proteínas.
DE102014212639A1 (de) * 2014-06-30 2015-12-31 Henkel Ag & Co. Kgaa Reinigungsmittel enthaltend Proteasen
WO2016069563A1 (en) * 2014-10-27 2016-05-06 Danisco Us Inc. Serine proteases
WO2016099917A1 (en) 2014-12-16 2016-06-23 Danisco Us Inc Enhanced protein expression
US20170369537A1 (en) 2014-12-19 2017-12-28 Danisco Us Inc. Enhanced protein expression
AR104783A1 (es) 2015-05-08 2017-08-16 Novozymes As VARIANTES DE a-AMILASA Y POLINUCLEÓTIDOS QUE LAS CODIFICAN
WO2016180749A1 (en) 2015-05-08 2016-11-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
WO2017089366A1 (en) * 2015-11-24 2017-06-01 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CN109563450A (zh) * 2016-05-31 2019-04-02 诺维信公司 稳定的液体过氧化物组合物
DE102016210628A1 (de) * 2016-06-15 2017-12-21 Henkel Ag & Co. Kgaa Bacillus gibsonii Protease und Varianten davon
DK3585910T3 (da) 2017-02-24 2024-06-17 Danisco Us Inc Sammensætninger og fremgangsmåder til forøget proteinproduktion i bacillus licheniformis
WO2019040412A1 (en) 2017-08-23 2019-02-28 Danisco Us Inc METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS
WO2019055261A1 (en) 2017-09-13 2019-03-21 Danisco Us Inc MODIFIED 5 'NON-TRANSLATED REGION (UTR) SEQUENCES FOR INCREASED PROTEIN PRODUCTION IN BACILLUS
WO2019089898A1 (en) 2017-11-02 2019-05-09 Danisco Us Inc Freezing point depressed solid matrix compositions for melt granulation of enzymes
CN111670244B (zh) 2018-01-03 2023-08-01 丹尼斯科美国公司 突变的和遗传修饰的芽孢杆菌属细胞及其用于增加蛋白质产生的方法
US20200032178A1 (en) * 2018-07-27 2020-01-30 The Procter & Gamble Company Water-soluble unit dose articles comprising water-soluble fibrous structures and particles
WO2021146411A1 (en) 2020-01-15 2021-07-22 Danisco Us Inc Compositions and methods for enhanced protein production in bacillus licheniformis
WO2022043547A1 (en) * 2020-08-28 2022-03-03 Novozymes A/S Protease variants with improved solubility
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
EP4388077A1 (en) 2021-08-20 2024-06-26 Danisco US Inc. Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations
CN121574967A (zh) * 2022-10-27 2026-02-27 青岛蔚蓝生物集团有限公司 碱性蛋白酶突变体及其应用
CN120712348A (zh) * 2023-03-06 2025-09-26 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1240058A (en) * 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
GB1353317A (en) 1970-03-03 1974-05-15 Koninklijke Gist Spiritus Enzyme-polymer complexes
GB1519148A (en) * 1974-11-19 1978-07-26 Gist Brocades Nv Compositions of matter
US4318818A (en) 1979-11-09 1982-03-09 The Procter & Gamble Company Stabilized aqueous enzyme composition
JPS6055118B2 (ja) 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
GB8311314D0 (en) 1983-04-26 1983-06-02 Unilever Plc Aqueous enzyme-containing compositions
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
US4511490A (en) * 1983-06-27 1985-04-16 The Clorox Company Cooperative enzymes comprising alkaline or mixtures of alkaline and neutral proteases without stabilizers
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
WO1986002053A1 (fr) 1984-10-05 1986-04-10 Zahnradfabrik Friedrichshafen Ag Servosysteme de direction, surtout pour vehicules motorises
ATE77649T1 (de) 1985-04-15 1992-07-15 Procter & Gamble Fluessige reinigungsmittel mit einer oberflaechenaktiven verbindung, einem proteolytischen enzym und borsaeure.
NZ216792A (en) 1985-07-26 1989-04-26 Colgate Palmolive Co Stabilised,fabric-softening built detergent compositions containing enzymes and swelling bentonite clay
US4771003A (en) 1985-10-22 1988-09-13 Genex Corporation Heat stable alkaline proteases produced by a bacillus
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
JPS63502959A (ja) * 1986-02-12 1988-11-02 ジェネックス、コ−ポレ−ション 突然変異誘発及びスクリ−ニング方法並びに生成物
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
SG30639G (en) * 1986-04-30 1995-09-01 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
EP0283075B1 (en) * 1987-02-27 1999-05-12 Genencor International, Inc. Molecular cloning and expression of genes encoding proteolytic enzymes
EP0353250B1 (en) * 1987-04-06 1999-09-08 Novo Nordisk A/S The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer

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EP0571049B2 (en) 2008-09-03
KR970005249B1 (ko) 1997-04-14
KR900700597A (ko) 1990-08-16
EP0328229B1 (en) 1994-01-19
AU3050189A (en) 1989-09-06
EP0571049B1 (en) 2003-09-24
US5336611A (en) 1994-08-09
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IE63248B1 (en) 1995-04-05
DK175813B1 (da) 2005-03-07
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ES2061929T3 (es) 1994-12-16
WO1989007642A1 (en) 1989-08-24
DE68912359D1 (de) 1994-03-03
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