LT3963B - Enzymatic product containing mutant proteolytic enzyme, process for selecting proteolytic enzyme, mutant gene, expression vector, procariotic host-strain, process for preparing mutant proteolytic enzyme, detergent composition - Google Patents
Enzymatic product containing mutant proteolytic enzyme, process for selecting proteolytic enzyme, mutant gene, expression vector, procariotic host-strain, process for preparing mutant proteolytic enzyme, detergent composition Download PDFInfo
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- LT3963B LT3963B LTIP1650A LTIP1650A LT3963B LT 3963 B LT3963 B LT 3963B LT IP1650 A LTIP1650 A LT IP1650A LT IP1650 A LTIP1650 A LT IP1650A LT 3963 B LT3963 B LT 3963B
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- Prior art keywords
- protease
- amino acid
- mutant
- enzyme
- enzymatic product
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- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Detergent Compositions (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP88200255 | 1988-02-11 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LTIP1650A LTIP1650A (en) | 1995-07-25 |
| LT3963B true LT3963B (en) | 1996-05-27 |
Family
ID=8199752
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTIP1650A LT3963B (en) | 1988-02-11 | 1993-12-21 | Enzymatic product containing mutant proteolytic enzyme, process for selecting proteolytic enzyme, mutant gene, expression vector, procariotic host-strain, process for preparing mutant proteolytic enzyme, detergent composition |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US5336611A (es) |
| EP (3) | EP0328229B2 (es) |
| JP (1) | JP2849141B2 (es) |
| KR (1) | KR970005249B1 (es) |
| CN (1) | CN1056187C (es) |
| AT (1) | ATE250669T1 (es) |
| AU (1) | AU629814B2 (es) |
| BG (1) | BG60566B1 (es) |
| BR (1) | BR8905580A (es) |
| CA (1) | CA1340366C (es) |
| DE (2) | DE68929484T3 (es) |
| DK (1) | DK175813B1 (es) |
| ES (1) | ES2061929T5 (es) |
| FI (1) | FI105481B (es) |
| GR (1) | GR3021934T3 (es) |
| IE (2) | IE63248B1 (es) |
| LT (1) | LT3963B (es) |
| LV (1) | LV10652B (es) |
| PT (1) | PT89702B (es) |
| RU (1) | RU2069695C1 (es) |
| WO (1) | WO1989007642A1 (es) |
Families Citing this family (125)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US5185258A (en) † | 1984-05-29 | 1993-02-09 | Genencor International, Inc. | Subtilisin mutants |
| JP2731407B2 (ja) * | 1987-04-03 | 1998-03-25 | アムジエン・インコーポレーテツド | 新規なプロテアーゼ酵素 |
| US6287841B1 (en) | 1988-02-11 | 2001-09-11 | Genencor International, Inc. | High alkaline serine protease |
| JPH04500385A (ja) * | 1989-06-26 | 1992-01-23 | ユニリーバー・ナームローゼ・ベンノートシヤープ | 酵素洗剤組成物 |
| US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
| DK0414297T3 (da) * | 1989-08-11 | 1997-07-07 | Genencor Int | Effektiv fremstilling af mutantproteaser |
| DE4023458A1 (de) * | 1989-08-31 | 1991-03-07 | Kali Chemie Ag | Neue hochalkalische proteasen |
| US5352603A (en) * | 1989-08-31 | 1994-10-04 | Kali-Chemie Ag | Highly alkaline proteases |
| US6271012B1 (en) * | 1989-10-11 | 2001-08-07 | Genencor International, Inc. | Protease muteins and their use in detergents |
| DK97190D0 (da) * | 1990-04-19 | 1990-04-19 | Novo Nordisk As | Oxidationsstabile detergentenzymer |
| US5733473A (en) * | 1990-11-14 | 1998-03-31 | The Procter & Gamble Company | Liquid detergent composition containing lipase and protease |
| DK271490D0 (da) * | 1990-11-14 | 1990-11-14 | Novo Nordisk As | Detergentkomposition |
| DK0486073T3 (da) * | 1990-11-14 | 1996-12-09 | Procter & Gamble | Væskeformig detergentsammensætning, der indeholder lipase og protease |
| GB9027836D0 (en) * | 1990-12-21 | 1991-02-13 | Unilever Plc | Enzymes and enzymatic detergent compositions |
| US5482849A (en) * | 1990-12-21 | 1996-01-09 | Novo Nordisk A/S | Subtilisin mutants |
| DE4106525A1 (de) * | 1991-03-01 | 1992-09-10 | Solvay Enzymes Gmbh & Co Kg | Verfahren zur verbesserung der stabilitaet von hochalkalischen proteasen |
| US5863783A (en) * | 1991-03-27 | 1999-01-26 | Gist-Brocades, N.V. | Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin |
| DK58391D0 (da) * | 1991-04-03 | 1991-04-03 | Novo Nordisk As | Hidtil ukendte proteaser |
| EP0583339B1 (en) * | 1991-05-01 | 1998-07-08 | Novo Nordisk A/S | Stabilized enzymes and detergent compositions |
| US5340735A (en) * | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
| US5510048A (en) * | 1991-05-31 | 1996-04-23 | Colgate Palmolive Co. | Nonaqueous liquid, phosphate-free, improved autoamatic dishwashing composition containing enzymes |
| EP0525610A3 (en) * | 1991-07-27 | 1993-03-24 | Solvay Enzymes Gmbh & Co. Kg | Process for increasing the stability of enzymes and stabilized enzymes |
| US5792623A (en) * | 1992-04-06 | 1998-08-11 | Immuno Aktiengesellschaft | Method for producing activated blood factors with a protease and a detergent |
| AT397390B (de) * | 1992-04-06 | 1994-03-25 | Immuno Ag | Verfahren zur spaltung von proteinen |
| EP0571014B1 (en) * | 1992-05-18 | 2004-03-31 | Genencor International, Inc. | Bacteria producing alkaline proteases, and production of these alkaline proteases |
| ATE376061T1 (de) * | 1992-07-17 | 2007-11-15 | Genencor Int | Hochalkalische serinproteasen |
| DE69334295D1 (de) * | 1992-07-23 | 2009-11-12 | Novo Nordisk As | MUTIERTE -g(a)-AMYLASE, WASCHMITTEL UND GESCHIRRSPÜLMITTEL |
| US5989887A (en) * | 1992-11-25 | 1999-11-23 | Dsm N.V. | Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin |
| DE4304161A1 (de) * | 1993-02-12 | 1994-08-25 | Solvay Enzymes Gmbh & Co Kg | Verbesserte hochalkalische Proteasen |
| US6436690B1 (en) | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
| US6440717B1 (en) * | 1993-09-15 | 2002-08-27 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis |
| AU8079794A (en) * | 1993-10-14 | 1995-05-04 | Procter & Gamble Company, The | Protease-containing cleaning compositions |
| US5932532A (en) * | 1993-10-14 | 1999-08-03 | Procter & Gamble Company | Bleach compositions comprising protease enzyme |
| US5677272A (en) * | 1993-10-14 | 1997-10-14 | The Procter & Gamble Company | Bleaching compositions comprising protease enzymes |
| MA23346A1 (fr) | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
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