KR930001117B1 - Dna 재조합 기술에 의한 폴리펩티드 제조방법 - Google Patents

Dna 재조합 기술에 의한 폴리펩티드 제조방법 Download PDF

Info

Publication number
KR930001117B1
KR930001117B1 KR1019850008020A KR850008020A KR930001117B1 KR 930001117 B1 KR930001117 B1 KR 930001117B1 KR 1019850008020 A KR1019850008020 A KR 1019850008020A KR 850008020 A KR850008020 A KR 850008020A KR 930001117 B1 KR930001117 B1 KR 930001117B1
Authority
KR
South Korea
Prior art keywords
dna
gene
polypeptide
yeast
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
KR1019850008020A
Other languages
English (en)
Korean (ko)
Other versions
KR870003197A (ko
Inventor
워어맥크 스트로우먼 데이빗드
프레드릭 브러스트 폴
브래들리 엘리스 스티븐
레이몬드 진저러스 토마스
밀러 하아폴 마이클
프리드리히 초프 유에르크
Original Assignee
휘립프스 피트로오리암 캄파니
제이 이이 휘립프스
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27099454&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR930001117(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/666,391 external-priority patent/US4808537A/en
Application filed by 휘립프스 피트로오리암 캄파니, 제이 이이 휘립프스 filed Critical 휘립프스 피트로오리암 캄파니
Publication of KR870003197A publication Critical patent/KR870003197A/ko
Application granted granted Critical
Publication of KR930001117B1 publication Critical patent/KR930001117B1/ko
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
KR1019850008020A 1984-10-30 1985-10-29 Dna 재조합 기술에 의한 폴리펩티드 제조방법 Expired - Lifetime KR930001117B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US666.391 1984-10-30
US06/666,391 US4808537A (en) 1984-10-30 1984-10-30 Methanol inducible genes obtained from pichia and methods of use
US780102 1985-09-25
US06/780,102 US4855231A (en) 1984-10-30 1985-09-25 Regulatory region for heterologous gene expression in yeast
US780.102 1985-09-25

Publications (2)

Publication Number Publication Date
KR870003197A KR870003197A (ko) 1987-04-15
KR930001117B1 true KR930001117B1 (ko) 1993-02-18

Family

ID=27099454

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019850008020A Expired - Lifetime KR930001117B1 (ko) 1984-10-30 1985-10-29 Dna 재조합 기술에 의한 폴리펩티드 제조방법

Country Status (23)

Country Link
US (1) US4855231A (https=)
EP (2) EP0483115A3 (https=)
JP (5) JP2502508B2 (https=)
KR (1) KR930001117B1 (https=)
CN (1) CN1011243B (https=)
AR (1) AR242988A1 (https=)
AT (1) ATE83263T1 (https=)
AU (1) AU572002B2 (https=)
CA (1) CA1340733C (https=)
DD (1) DD254211A5 (https=)
DE (1) DE3586889T2 (https=)
DK (1) DK496385A (https=)
ES (1) ES8609458A1 (https=)
FI (1) FI94427C (https=)
GR (1) GR852611B (https=)
HU (1) HU205627B (https=)
IE (1) IE930804L (https=)
IL (1) IL76765A (https=)
NO (1) NO178076C (https=)
NZ (1) NZ213842A (https=)
PL (1) PL158965B1 (https=)
PT (1) PT81399B (https=)
YU (1) YU46695B (https=)

Families Citing this family (117)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
IL81817A0 (en) * 1986-03-14 1987-10-20 Phillips Petroleum Co Methanol and glucose responsive yeast regulatory regions
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
DE3789866T2 (de) * 1987-07-17 1994-09-22 Rhein Biotech Gesellschaft Fuer Neue Biotechnologische Prozesse Und Produkte Mbh, 40595 Duesseldorf DNA-Moleküle, die für FMDH-Kontrollabschnitte und Strukturgene für ein Protein mit FMDH-Aktivität kodieren, sowie deren Anwendung.
ES2204884T3 (es) * 1988-01-05 2004-05-01 Roche Diagnostics Gmbh Procedimiento para la obtencion de proteinas o productos genicos que contienen proteinas.
SE8901264L (sv) * 1988-04-11 1989-10-12 Phillips Petroleum Co Uttryck av laxtillvaexthormon i metylotropisk jaest av slaektet pichia
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
IL89989A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human interleukin-2 in methylotrophic yeasts
NZ228774A (en) * 1988-04-25 1991-05-28 Phillips Petroleum Co Hbv particles containing pres 2 proteins and recombinant processes for their production in yeast cells
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
IL89992A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
ATE111524T1 (de) * 1988-12-20 1994-09-15 Rhein Biotech Ges Fuer Biotech Mutanter stamm von einem methylotrophen organismus und verfahren zur herstellung eines proteins in methylotrophen organismus.
AU5196290A (en) * 1989-02-13 1990-09-05 Salk Institute Biotechnology/Industrial Associates, Inc., The Production of superoxide dismutase in pichia pastoris yeast cells
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
US6440681B1 (en) 1990-04-03 2002-08-27 Merck & Co., Inc. Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors
US5369028A (en) * 1990-04-03 1994-11-29 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same
US5258302A (en) * 1990-07-03 1993-11-02 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA for expression of aprotinin in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US5834262A (en) * 1992-01-06 1998-11-10 E. I. Du Pont De Nemours And Company Oxidation of glycolic acid to glyoxylic acid using a microbial cell transformant as catalyst
JP3113435B2 (ja) * 1992-02-28 2000-11-27 サントリー株式会社 メタノールおよび／またはグリセロール添加により誘導可能なプロモーターを有する新規ベクター
US5708141A (en) * 1992-05-11 1998-01-13 Corvas International, Inc. Neutrophil inhibitors
HUT73214A (en) * 1993-03-03 1996-06-28 Du Pont Production of glycolate oxidase in methylotrophic yeast
DE69434067T2 (de) * 1993-03-08 2006-03-02 Merck & Co., Inc. Menschliche neuronale nikotin-acetylcholin-rezeptor-verbindungen und methoden ihres einsatzes
WO1994021802A1 (en) * 1993-03-25 1994-09-29 Biosource Genetics Corporation PICHIA PASTORIS ALCOHOL OXIDASE ZZA1 and $i(ZZA2)
EP0696320B1 (en) * 1993-04-20 2002-09-25 Merck & Co., Inc. Human n-methyl-d-aspartate receptor subunits, nucleic acids encoding same and uses therefor
US6001581A (en) 1993-06-04 1999-12-14 Sibia Neurosciences, Inc. Cation-based bioassay using human metabotropic glutamate receptors
US5521297A (en) 1993-06-04 1996-05-28 Salk Institute Biotechnology/Industrial Associates Nucleic acids encoding human metabotropic glutamate receptors
US5912122A (en) 1993-06-04 1999-06-15 Sibia Neurosciences, Inc. Nucleic acids encoding and method for detecting nucleic acid encoding human metabotropic glutamate receptor subtype mGluR6
US6495343B1 (en) 1993-06-18 2002-12-17 The Salk Institute For Biological Studies Cloning and recombinant production of CRF receptor(s)
US6638905B2 (en) * 1993-06-18 2003-10-28 The Salk Institute For Biological Studies Cloning and recombinant production of CFR receptor(s)
WO1995013299A1 (en) * 1993-11-08 1995-05-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
US5453161A (en) * 1993-11-12 1995-09-26 Enichem S.P.A. Polyamic acid to polyimide conversion by microwave heating
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
EP0788546B9 (en) * 1994-10-18 2007-06-13 Dendreon Corporation Nematode-extracted serine protease inhibitors and anticoagulant proteins
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6485967B1 (en) 1995-06-07 2002-11-26 Merck & Co., Inc. Human neuronal nicotinic acetylcholine receptor α6 and β3 nucleic acid
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US5965389A (en) * 1995-11-09 1999-10-12 Zymogenetics, Inc. Production of GAD65 in methylotrophic yeast
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
EP0911416B1 (en) 1997-10-01 2006-05-17 DSM IP Assets B.V. Protein production process
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
EP1025217B1 (en) * 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
JP4524816B2 (ja) * 1998-07-06 2010-08-18 東ソー株式会社 Ｉｌ−６レセプター・ｉｌ−６直結融合蛋白質
DE69941406D1 (de) * 1998-07-06 2009-10-22 Tosoh Corp IL-6 Rezeptor IL-6-gekoppeltes Fusionsprotein
AU5584999A (en) * 1998-08-28 2000-03-21 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6780615B1 (en) 1998-12-31 2004-08-24 Genway Biotech Inc. Production of recombinant monellin using methylotrophic yeast expression system
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
EP1173460B1 (en) * 1999-03-02 2009-09-16 Life Technologies Corporation Compositions and methods for use in recombinational cloning of nucleic acids
US7504253B2 (en) * 1999-06-11 2009-03-17 The Burnham Institute For Medical Research Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof
US6440414B1 (en) * 1999-10-01 2002-08-27 Amgen Inc. Pharmaceutical compositions of fibrinolytic agent
US6261820B1 (en) * 1999-10-01 2001-07-17 Amgen Inc. Fibronolytically active polypeptide
RU2002112751A (ru) 1999-11-12 2004-02-27 Файброджен, Инк. (Us) Рекомбинантные желатины
NZ539430A (en) 1999-12-10 2006-09-29 Invitrogen Corp Use of multiple recombination sites with unique specificity in recombinational cloning
US7033776B2 (en) * 1999-12-17 2006-04-25 Amgen Inc. Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
WO2001077351A1 (en) * 2000-04-07 2001-10-18 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vectors and methods for dual protein expression in pichia pastoris and escherichia coli
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
ES2252261T3 (es) * 2000-06-28 2006-05-16 Glycofi, Inc. Metodos para producir glicoproteinas modificadas.
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
EP1294910B1 (en) 2000-06-30 2008-11-19 VIB vzw Protein glycosylation modification in pichia pastoris
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1385866A4 (en) * 2001-04-05 2006-03-29 Univ Nebraska ALCOHOL OXIDASE 1 REGULATORY NUCLEOTIDE SEQUENCES FOR THE EXPRESSION OF HETEROLOGOUS GENES IN YEAST
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
WO2003089589A2 (en) * 2002-04-15 2003-10-30 Merck & Co., Inc. Matrix analysis of gene expression in cells (magec)
IL165717A0 (en) 2002-06-26 2006-01-15 Flanders Interuniversity Inst A strain of methylotrophic yeast for producing proteins
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
DE60335602D1 (de) * 2002-12-18 2011-02-17 Roche Diagnostics Gmbh Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
ATE387494T1 (de) * 2002-12-20 2008-03-15 Hoffmann La Roche Hitzelabile desoxyribonuklease i-varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
EP1673457B1 (en) * 2003-08-25 2011-05-18 Funzyme Biotechnologies SA Novel fungal proteins and nucleic acids encoding same
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
ES2337684T3 (es) * 2003-12-05 2010-04-28 F. Hoffmann-La Roche Ag Carboxipeptidasa b recombinante y su purificacion.
AU2006291780B2 (en) * 2005-09-14 2011-12-08 F. Hoffmann-La Roche Ag Cleavage of precursors of insulins by a variant of trypsin
AT504588B1 (de) * 2006-11-16 2008-08-15 Univ Graz Tech Nukleinsäure-promotor
BRPI0910286A2 (pt) 2008-03-03 2015-09-29 Abbott Lab métodos para transformação de levedura
KR20110101198A (ko) 2008-12-12 2011-09-15 이 아이 듀폰 디 네모아 앤드 캄파니 재생 가능 자원으로부터의 선형 다이카르복실산의 제조 방법
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
US20120276075A1 (en) 2009-12-21 2012-11-01 Centre Hospitalier Universitaire Vaudois (Chuv) Synergic action of a prolyl protease and tripeptidyl proteases
MX368546B (es) 2011-05-20 2019-10-07 Alderbio Holdings Llc Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris.
SG10201609752YA (en) 2011-08-19 2017-01-27 Alderbio Holdings Llc Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris
EP2764016B1 (en) 2011-10-07 2019-03-13 Lonza Ltd Regulatable promoter
CA2905180C (en) 2013-03-15 2023-03-07 Alder Biopharmaceuticals, Inc. Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
DK2970994T3 (da) 2013-03-15 2019-10-28 Lonza Ag Konstitutiv promotor
TWI670279B (zh) 2013-03-15 2019-09-01 Alder Biopharmaceuticals, Inc. 抗體純化及純度監測
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
WO2014146175A1 (en) 2013-03-21 2014-09-25 Commonwealth Scientific And Industrial Research Organisation Purification of triple helical proteins
EP2999712B1 (en) 2013-04-30 2018-11-21 Donald Danforth Plant Science Center Antifungal plant proteins, peptides, and methods of use
CN105764920B (zh) 2013-09-09 2020-07-24 联邦科学工业研究组织 修饰的细菌胶原蛋白样蛋白
WO2016120764A1 (en) 2015-01-26 2016-08-04 Centre Hospitalier Universitaire Vaudois (Chuv) Aspergillus oryzae prolyl endopeptidases and use thereof in degradation of polypeptides
WO2021126129A1 (en) * 2019-12-19 2021-06-24 Atatürk Üni̇versi̇tesi̇ Bi̇li̇msel Araştirma Projeleri̇ Bi̇ri̇mi̇ A method for recombinant protein production

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4615974A (en) * 1981-08-25 1986-10-07 Celltech Limited Yeast expression vectors
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
JPS59501572A (ja) * 1982-05-19 1984-09-06 ユニリーバー　ナームローゼ　ベンノートシヤープ クルイベロミセス属酵母およびその製造法
EP0118551A1 (en) * 1982-09-15 1984-09-19 Collaborative Research Inc. Production of interferon in yeast by use of invertase promoter
CA1341130C (en) * 1984-07-27 2000-10-31 Adrianus Marinus Ledeboer Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia

Also Published As

Publication number Publication date
IL76765A (en) 1991-06-30
PL158965B1 (pl) 1992-10-30
PL256012A1 (en) 1986-09-23
IE930804L (en) 1986-04-30
JPH08196275A (ja) 1996-08-06
PT81399B (pt) 1987-11-11
EP0183071A2 (en) 1986-06-04
JPH08173161A (ja) 1996-07-09
FI94427C (fi) 1995-09-11
EP0183071A3 (en) 1987-09-23
JP2502508B2 (ja) 1996-05-29
PT81399A (en) 1985-11-01
CN1011243B (zh) 1991-01-16
ATE83263T1 (de) 1992-12-15
AR242988A1 (es) 1993-06-30
CA1340733C (en) 1999-09-14
GR852611B (https=) 1986-03-04
JP2552808B2 (ja) 1996-11-13
HU205627B (en) 1992-05-28
FI854142L (fi) 1986-05-01
NO178076C (no) 1996-01-17
AU572002B2 (en) 1988-04-28
NO854312L (no) 1986-05-02
NO178076B (no) 1995-10-09
YU169585A (en) 1991-04-30
FI94427B (fi) 1995-05-31
JP2552807B2 (ja) 1996-11-13
US4855231A (en) 1989-08-08
JPH0698789A (ja) 1994-04-12
DE3586889D1 (de) 1993-01-21
ES548307A0 (es) 1986-09-01
EP0183071B1 (en) 1992-12-09
CN85109718A (zh) 1986-08-20
DK496385D0 (da) 1985-10-29
JPS61173781A (ja) 1986-08-05
DE3586889T2 (de) 1993-04-15
YU46695B (sh) 1994-04-05
FI854142A0 (fi) 1985-10-23
AU4875285A (en) 1986-06-12
ES8609458A1 (es) 1986-09-01
DK496385A (da) 1986-05-01
NZ213842A (en) 1991-11-26
JPH06233686A (ja) 1994-08-23
KR870003197A (ko) 1987-04-15
EP0483115A2 (en) 1992-04-29
DD254211A5 (de) 1988-02-17
HUT38678A (en) 1986-06-30
EP0483115A3 (en) 1992-05-06

Similar Documents

Publication Publication Date Title
KR930001117B1 (ko) Dna 재조합 기술에 의한 폴리펩티드 제조방법
US4808537A (en) Methanol inducible genes obtained from pichia and methods of use
CA1283373C (en) Use of the gal 1 yeast promoter
KR0181179B1 (ko) 피치아 파스토리스 산성 인산효소 유전자의 디앤에이 단편 및 이를 사용하는 방법
EP0263311A2 (en) Yeast production of human tumor necrosis factor
EP0020147B1 (en) A dna transfer vector for human pre-growth hormone, a microorganism transformed thereby, and a method of cloning therefor
US5795776A (en) Expression plasmids regulated by an OSMB promoter
GB2091271A (en) Recombinant dna
JPH0253489A (ja) メチロトローフ酵母のアルコールオキシダーゼ２調節領域をコードするｄｎａ配列
EP0143834A1 (en) Heat regulated production of selected and fused proteins in yeast
IE81158B1 (en) Expression of hepatitis b s and pres2 proteins in methylotrophic yeasts
WO1984001153A1 (en) Production of interferon in yeast by use of invertase promoter
US5310660A (en) Method and a hybrid promoter for controlling exogenous gene transcription
KR100393297B1 (ko) 돌연변이체aox2프로모터,이를보유한벡터,형질전환체및이종단백질의제조방법
EP0339567A1 (en) Expression of Hepatitis B PreS 2 Protein in Methylotrophic Yeasts
US5217880A (en) L-fucose dehydrogenase gene, microorganism having said gene and production of l-fucose dehydrogenase by the use of said microorganism
CA2012025C (en) Dna for expression and secretion
US5496711A (en) Processes for producing pre-prorennin, prorennin and rennin
CA1156166A (en) Recombinant bacterial plasmids containing the coding sequences of insulin genes
CA1273883A (en) Recombinant dna material comprising bovine growth hormone nucleotide sequence
JPH02501260A (ja) 合成遺伝子を使用した酵母細胞でのブタ成長ホルモンの生産方法

Legal Events

Date Code Title Description
PA0109 Patent application

Patent event code: PA01091R01D

Comment text: Patent Application

Patent event date: 19851029

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 19870929

Comment text: Request for Examination of Application

Patent event code: PA02011R01I

Patent event date: 19851029

Comment text: Patent Application

E601 Decision to refuse application
E902 Notification of reason for refusal
PE0601 Decision on rejection of patent

Patent event date: 19900331

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 19900331

Patent event code: PE09021S01D

J2X1 Appeal (before the patent court)

Free format text: APPEAL AGAINST DECISION TO DECLINE REFUSAL

PJ2001 Appeal

Appeal kind category: Appeal against decision to decline refusal

Decision date: 19921221

Appeal identifier: 1991201000722

Request date: 19910513

G160 Decision to publish patent application
PG1605 Publication of application before grant of patent

Comment text: Decision on Publication of Application

Patent event code: PG16051S01I

Patent event date: 19930120

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 19930430

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 19930623

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 19930623

End annual number: 3

Start annual number: 1

PR1001 Payment of annual fee

Payment date: 19960117

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 19961228

Start annual number: 5

End annual number: 5

PR1001 Payment of annual fee

Payment date: 19971230

Start annual number: 6

End annual number: 6

PR1001 Payment of annual fee

Payment date: 19990528

Start annual number: 7

End annual number: 7

PR1001 Payment of annual fee

Payment date: 20000108

Start annual number: 8

End annual number: 8

PR1001 Payment of annual fee

Payment date: 20010118

Start annual number: 9

End annual number: 9

PR1001 Payment of annual fee

Payment date: 20020123

Start annual number: 10

End annual number: 10

PR1001 Payment of annual fee

Payment date: 20030116

Start annual number: 11

End annual number: 11

PR1001 Payment of annual fee

Payment date: 20040112

Start annual number: 12

End annual number: 12

FPAY Annual fee payment

Payment date: 20050107

Year of fee payment: 13

PR1001 Payment of annual fee

Payment date: 20050107

Start annual number: 13

End annual number: 13

EXPY Expiration of term
PC1801 Expiration of term