KR20140122729A - 태그로서 리소자임의 용도 - Google Patents

태그로서 리소자임의 용도 Download PDF

Info

Publication number
KR20140122729A
KR20140122729A KR1020147023426A KR20147023426A KR20140122729A KR 20140122729 A KR20140122729 A KR 20140122729A KR 1020147023426 A KR1020147023426 A KR 1020147023426A KR 20147023426 A KR20147023426 A KR 20147023426A KR 20140122729 A KR20140122729 A KR 20140122729A
Authority
KR
South Korea
Prior art keywords
protein
lysozyme
polypeptide
tag
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020147023426A
Other languages
English (en)
Korean (ko)
Inventor
스테판 하엘트레
세바스티안 자에거
다니엘라 다우베르트
Original Assignee
모르포시스 아게
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 모르포시스 아게 filed Critical 모르포시스 아게
Publication of KR20140122729A publication Critical patent/KR20140122729A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020147023426A 2012-01-23 2013-01-23 태그로서 리소자임의 용도 Ceased KR20140122729A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261589408P 2012-01-23 2012-01-23
US61/589,408 2012-01-23
EP12152095.1 2012-01-23
EP12152095 2012-01-23
PCT/EP2013/051181 WO2013110627A1 (en) 2012-01-23 2013-01-23 Use of lysozyme as a tag

Publications (1)

Publication Number Publication Date
KR20140122729A true KR20140122729A (ko) 2014-10-20

Family

ID=48872896

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020147023426A Ceased KR20140122729A (ko) 2012-01-23 2013-01-23 태그로서 리소자임의 용도

Country Status (10)

Country Link
US (1) US9689014B2 (https=)
EP (1) EP2807179B1 (https=)
JP (1) JP6215231B2 (https=)
KR (1) KR20140122729A (https=)
CN (1) CN104066745B (https=)
AU (1) AU2013211651C1 (https=)
CA (1) CA2860916A1 (https=)
ES (1) ES2582802T3 (https=)
IN (1) IN2014DN06660A (https=)
WO (1) WO2013110627A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105524942A (zh) * 2016-02-05 2016-04-27 广州赛莱拉干细胞科技股份有限公司 一种表达载体及胰岛素样生长因子结合蛋白3的表达方法
US10986850B2 (en) 2016-12-21 2021-04-27 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
WO2018113745A1 (en) * 2016-12-21 2018-06-28 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
CN107794254A (zh) * 2017-09-30 2018-03-13 昆明理工大学 重组高温裂解酶的快速纯化方法
WO2022133068A1 (en) * 2020-12-16 2022-06-23 Danisco Us Inc Enzymes and enzyme compositions for cleaning
CN118974227A (zh) * 2022-03-01 2024-11-15 丹尼斯科美国公司 用于清洁的酶和酶组合物
WO2025179145A1 (en) * 2024-02-23 2025-08-28 Dualitas Therapeutics, Inc. Bispecific screening compositions and methods

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2459701A (en) 1945-07-16 1949-01-18 Harper Esther Pardee Mechanical instrument holder
US4355022A (en) 1981-07-01 1982-10-19 Interon, Inc. Method of dental treatment
JP2761881B2 (ja) 1988-03-10 1998-06-04 チッソ株式会社 抗体を固定化したアフイニテイクロマトグラフイ用担体
DE69024323T2 (de) 1989-10-27 1996-10-17 Genencor Int Antimikrobielles Verfahren und Formulierung unter Verwendung von Endoglycosidase vom Typ II und antimikrobielles Mittel
EP1007842B1 (de) 1997-08-22 2004-12-15 Siemens Aktiengesellschaft Verfahren zur klopfregelung einer brennkraftmaschine
PL347250A1 (en) 1998-10-15 2002-03-25 Dsm Nv Antimicrobial enzymes in animal feed
EP1194570A1 (en) 1999-06-23 2002-04-10 PPL Therapeutics (Scotland) Limited Fusion proteins incorporating lysozyme
CA2472477C (en) 2002-01-07 2012-03-20 Lifesensors, Inc. Methods and compositions for protein expression and purification
DE10237317B4 (de) 2002-08-15 2010-04-08 3M Espe Ag Enzymhaltige Zusammensetzung, Verfahren zu deren Herstellung und deren Verwendung
CA2404356A1 (en) 2002-09-18 2004-03-18 Canadian Inovatech Inc. Gram-positive antibacterial composition and method for use
US7428273B2 (en) 2003-09-18 2008-09-23 Promptu Systems Corporation Method and apparatus for efficient preamble detection in digital data receivers
JP4891220B2 (ja) 2004-02-25 2012-03-07 ノボザイムス アクティーゼルスカブ 菌類細胞壁分解酵素
PL390235A1 (pl) 2007-04-10 2010-07-05 Saint Simeon Ldasaint Simeon Lda Nowe kompozycje zawierające lizozym i polisacharydy C-1/C-4 i ich zastosowanie do pielęgnacji jamy ustnej, w kosmetologii i dermatologii, antykoncepcji, urologii i ginekologii
AU2008311869B2 (en) 2007-10-17 2013-11-21 The Board Of Trustees Of The Leland Stanford Junior University Method and composition for crystallizing G protein-coupled receptors
CN101817883B (zh) * 2010-03-12 2012-06-13 广东药学院 一种家蝇天蚕素-人溶菌酶融合蛋白及其制备方法和应用

Also Published As

Publication number Publication date
JP2015504683A (ja) 2015-02-16
CN104066745A (zh) 2014-09-24
AU2013211651B2 (en) 2017-03-02
WO2013110627A1 (en) 2013-08-01
US9689014B2 (en) 2017-06-27
EP2807179A1 (en) 2014-12-03
AU2013211651C1 (en) 2017-10-12
EP2807179B1 (en) 2016-04-20
JP6215231B2 (ja) 2017-10-18
CN104066745B (zh) 2017-04-12
CA2860916A1 (en) 2013-08-01
US20140349343A1 (en) 2014-11-27
AU2013211651A1 (en) 2014-07-24
HK1202298A1 (en) 2015-09-25
ES2582802T3 (es) 2016-09-15
IN2014DN06660A (https=) 2015-05-22

Similar Documents

Publication Publication Date Title
AU2019283892B2 (en) Anti-human papillomavirus 16 E7 T cell receptors
KR20140122729A (ko) 태그로서 리소자임의 용도
KR102551733B1 (ko) B형 혈우병 유전자 요법을 위한 증가된 발현을 가지는 재조합 fix 변이체를 암호화하는 바이러스 벡터
KR101078202B1 (ko) 재조합 루브리신 분자 및 이의 용도
US20020102644A1 (en) Pharmaceutical compositions comprising herpes virus entry receptor protein
CN106544361B (zh) 哺乳动物细胞表达载体、表达系统、制备方法和应用
CN106591371A (zh) Cd16a/gpc3双抗慢病毒表达载体及其构建方法和应用
JP2001224388A (ja) ヒトプロカルシトニンならびにその製造および使用
CN108913718A (zh) 一种靶向EGFR vⅢ的CAR-T细胞的制备方法及应用
CN114540424A (zh) Igfbp7肌肉组织特异性敲除小鼠动物模型及其构建方法
CN112608940B (zh) 一种先天性白内障疾病动物模型构建方法及应用
CN113943737A (zh) 一种鸡ctgf基因在抑制鸡前脂肪细胞分化的应用
CN108103088B (zh) 重组GLP-1类似物Fc融合蛋白的优化基因及其应用
CN107474131A (zh) 一种重组牛血清蛋白成熟肽及其制备方法和应用
CN114150001A (zh) 一种用于弓形虫基因编辑的CRISPR/Cas9载体的构建方法
CN106978432B (zh) 敲除衣藻内源基因和表达外源基因的载体构建方法及应用
KR102009270B1 (ko) 구제역 O-Thi60 주의 방어 항원이 발현되는 재조합 바이러스
CN114395020B (zh) GmRALF1蛋白在促进植物对磷元素吸收中的应用
CN114134170A (zh) 一种ha标签融合表达载体的制备方法及其应用
KR101876488B1 (ko) Myh3 유전자를 포함하는 형질전환체 및 이의 이용
CN111100874B (zh) 打靶载体及整合外源基因至小鼠dc-sign外显子7位点构建bac克隆的方法和应用
KR102109820B1 (ko) 비스페놀 a와의 결합력이 향상된 돌연변이 에스트로겐 수용체 단백질 및 이를 이용한 박테리아 균주
CN108034678A (zh) 一种表达重组人神经生长因子的表达载体、体系及方法
CN111909957B (zh) 一种雨生红球藻的遗传转化方法
KR101246884B1 (ko) 지방산 생합성 경로의 과발현용 형질전환 슈도모나스 에어루지노사 및 그의 제조방법

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20140821

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20171114

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20181024

Patent event code: PE09021S01D

E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20190507

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20181024

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I